WUHAN HUAMEI BIOTECH Co., Ltd.
Address: Incubator Building 4th floor, Wuhan University Science
Park, No.te-1, Daxueyuan Road ,Donghu Hi-Tech Development
Area, Wuhan, Hubei Province 430223, P.R.China
Website:http://www.cusabio.com, http://www.cusabio.cn
Tel 86-27-87582341
Fax: 86-27-87196150
Email: cusabio@cusabio.com
Preparation of prokaryotic protein service contract
（This order is valid from
to
）
Order number ：
Order Date：
I． Customer Information
Name：
Company：
Telephone：
Fax：
Phone：
E-mail：
Address：
Postal Code：
II． Service information
Gene and protein information：
Gene name
GeneBank
accession number
Species
Length of coding
(
)p
sequences
Gene sequence of
Please detail in the electronic version
the target protein
Customer to provide cDNA (with sequence and vector information)
Gene sources
The company to synthesis the wanted gene
Other Manners (
Expression vector
Customer to provide the empty expression vector (with
).
sequence and
vector information)
We construct the wanted expression vector
The recombinant
Customer to provide (Please provide the target gene sequence and
expression vector
If the customers to
build a good
expression vector
vector information)
We construct the recombinant expression vector according to the
customers with the empty vector sequence and map.
Host strain name:
Containing plasmid:
Culture conditions:
Storage temperature:
purification method
Ni column affinity purification.
GST column affinity purification.
MBP column affinity purification.
Purity testing
methods
SDS-PAGE
Others (
Others (
)
Western Blot
).
Other explanatory
For what experiments (We will recommend suitable protein purification according to the purpose of
your experiment):
Please fill in :
The requirements of the ultimate goal of protein
The target protein without purification tag
Whether to fused
with tag
(Corresponding increase in costs and cycle).
The target protein can be fused with the following purification tag
His Tag
inclusion bodies,
refold or not
Protein demand
Delivery
requirements
Yes
(
GST
Other (
No
) mg purfied target protein
20mmol/LTris-HCl buffer (pH7.4)
Other buffer（
Other special
requirements, please
specify
MBP
PBS buffer
）
Lyophilized
)
III. Services Selection
Item Number
CSBYF0701
CSBYF0702
CSBYF0303
Payment
Item Description
Price
customers provide the
gene template
Working days
required
4-6 weeks
Synthesis gene
Protein required
removal of the tag or
protein refolding
5-7 weeks
Additional 1
weeks
Customer needs to pay 33% of total amount,
USD,as
prepayment on signing the contract and the 67% balance payment
USD ,when the project is completed.
V．Service Commitments
1-2 months
servic
e time Note:Cusabio will complete the related service in the above time limit since we receive the
prepayment of customer. We will provide with detailed report if we can’t complete
limit
in the due time. And decide a solution with the negotiation between both sides
servic
e
produ Provide customers with the required protein, if necessary, to
cts
provide the results of intermediate steps.
offere
d
other
servic
e
（1）startup the project once we receive the prepayment from customer；
comm （2）customer has the right to know the proiect status any time
ittme
nts
IV．Experiment Program and Data Provide
experimental procedure
Delivery results
Remarks
Gene to obtain: Using expression of PCR results
the gene, cell or tissue extract
mRNA, and reverse transcription
PCR, and to design appropriate
primers for amplification of the
target fragment, to obtain the target
gene. Or direct synthesis of the
target gene.
Cloning: the cleavage of the target
Target gene
gene fragment, connected to the
sequencing report
same restriction enzyme to deal
with the vector, and transformed
into E. coli DH5, positive clones
were screened.
Gene expression: extraction of
positive cloned plasmid was
transformed into expression host,
pick a single colony, and a small
amount of induced expression,
SDS-PAGE analysis of the target
gene expression.
Protein Purification: a large number
of inducible expression, the
expression host strain collection
medium lysis, affinity
chromatography purified protein.
The tag removal: the removal of
fusion protein tags.
If the customer provides the gene,
RT-PCR and gene synthesis step is
omitted, only from customer-supplied
template were subcloned.
SDS-PAGE
analysis results
SDS-PAGE
analysis results
SDS-PAGE
analysis results
Based on customer needs to select the
experiment
VI．The confidentiality agreement
CUSABIO must keep confidential on the custom services in contract. Without customer’s
permission, CUSABIO can’t leak any information about the service to the third party.
Note: This contract is in duplicate. Each party shall hold one copy. The fax copy is also legally
binding. If the contract is not completed as planned, both two parties should search for negotiated
settlement.
VII．Customer guidelines
·In the case does not affect the confidentiality of customer trials, as far as possible to provide more
detailed information of the protein.
·Please customize this service to provide samples to facilitate the accurate quotation for you.
·Due to experimental problems caused by customers to provide samples or reagents, the
responsibility of the customer.
The contract is in two copies, both parties hold one copy, which have the equal legal effect
To Customer:
To Company: WUHAN HUAMEI BIOTECH
Co., Ltd
Seal：：
Seal：
Signature：
Signature：
Date:
Date: