Molecular Biology Core Facility Pricing and Discounts* 3/2014 Integrated DNA Technologies (IDT) Oligonucleotides Scale Guaranteed Yield Length Restriction Price Per Base Purification (PAGE, HPLC) 25 nmole 2 ODs 15-60 bases $0.25 N/A 100 nmole 6 ODs 10-90 bases $0.45 $42 250 nmole 15 ODs 5-100 bases $0.80 $62 1 umole 40 ODs 5-100 bases $1.75 $103 ** 5 umole, 10 uM available PAGE ultramer $0.70/base, $99.75 PAGE 60-200 bases see IDT site for information on application When placing oligo orders to the MBCF, please specify the scale and any purification, if applicable Refer to www.idtdna.com for information on your specific needs No shipping or setup charges 5% off specialties, including dual labeled probes (Black Hole Quenchers for QPCR probes are available) Various 5’, internal and 3’ modifications available IDT Ready-Made Primers : IN STOCK The MBCF keeps oligo(dT), anchored oligo(dT), and random hexamers $10.00 Life Technologies No shipping or dry ice charges ; Discounts on many reagents NuPAGE Gels/Buffers: IN STOCK Catalog # Item Amount List price We charge NP0321BOX 4-12% BT gel, 1.0mm, 10 well 10 gels 142.00 140.56 NP0322BOX 4-12% BT gel, 1.0mm, 12 well 10 gels 142.00 139.16 NP0002 MES SDS running buffer, 20X 500 ml 71.50 71.50 NP0001 MOPS SDS running buffer, 20X 500 ml 71.50 71.50 NP0006-1 Transfer buffer, 20X 1 liter 156.00 156.00 *PRICES MAY CHANGE WITHOUT NOTICE Other Life Technolgies reagents: IN STOCK Catalog # Item Amount List price We charge 11730-025 Platinum qPCR supermix -UDG 500 rxns 1,016.00 934.72 18080-051 SuperScript III reverse transciptase 50 rxn 410.00 401.80 10977-015 Nuclease-free H2O 500 ml 29.00 29.00 -Molecular Probes and Applied Biosystems products can now be ordered through Life Technologies with no charge for shipping Bio-Rad All Bio-Rad products are discounted 5% and no shipping charges; equipment up to $2500.00. IN STOCK: Select Protein markers: Precision Plus Dual Color, Precision Plus All Blue and Kaleidoscope Refer to Ordering page of the MBCF web site for details QPCR: Optically clear tape ($1.36) and 96 well plates ($4.39) can be purchased individually through the MBCF for your QPCR runs. Promega Helix Freezer On-site stock of commonly used restriction enzymes, markers, reagents and kits Promega Freezer inventory and price lists are available on the Ordering page of the MBCF web site: www.etsu.edu/com/mbcf **Sign up to get your Promega Express Purchasing Pass Qiagen *Contact MBCF for current Agreement Code in order to get 5% discount on comsumables (some exceptions). Free shipping for orders over $500.00. **Place Qiagen orders through your department *-*-*-*-*-*-*-*-*-*-*-*-*-*-*mbcf@etsu.edu 423-439-8096