LESSON 4: Measuring Watershed Health Part II – Data Collection FIELDTRIP PRACTICE EXERCISES NOTE: The exercise described here is a portion of what is covered in Lesson 4: Measuring Watershed Health Part II – Data Collection (included in CFWEP’s Base-level curriculum). It is a follow-up of what was covered during Lesson 3: Measuring Watershed Health Part I – Biological Indicators. In lesson 3, students were introduced to: 1) the structural layers of the riparian vegetation; 2) categories of plant types found within each structural layer; and 3) the datasheet used during the vegetation assessment. Practice Vegetation Station Objectives: In this current lesson, which is part of Lesson 4: Measuring Watershed Health Part II – Data Collection, students are given the opportunity to practice how to use the equipment and complete the datasheet used for the riparian vegetation assessment. Materials* (Figure 1): Assembled pictures of structural layers of riparian vegetation (see below for picture assembly instructions) Tent pole (should extend to about 5 m long) Masking tape Scissors 30-m transect tape Practice datasheet for vegetation CFWEP Plant Guide for Kids *For classes with more than 10 students, double all materials and set-up two (2) transects. Procedure: Pictures representing the structural layers of the riparian vegetation are hung on the tent pole and taped to a wall, serving as a mock transect (Figure 2). The transect consists of 5 sets of pictures placed about 1 meter apart on the tent pole (Figure 2). 1 LESSON 4: Measuring Watershed Health Part II – Data Collection FIELDTRIP PRACTICE EXERCISES A set is made up of 3 pictures: the top picture is a common view of overstory components; the middle picture shows understory components; and the lower picture (resting on the ground) shows ground cover components (Figure 3). Secure with masking tape, the 30-m tape measure onto the ground cover pictures (Figure 4 and 5). Be sure to place the “1 m” mark in the center of the first ground cover picture; the “2 m” mark on the center of the second ground cover picture; and so on. 2 LESSON 4: Measuring Watershed Health Part II – Data Collection FIELDTRIP PRACTICE EXERCISES Direct students to collect data based on what they observe in the pictures using the practice datasheet (a modified version of the vegetation datasheet; Figure 6). Be sure to emphasize the use of the codes to record data (codes were explained to students in Lesson 3). After completion of both the practice vegetation and water quality stations, discussions follow. Directions for Assembling Pictures Materials: Pictures of structural layers of riparian vegetation o 5 each of overstory, understory and ground cover for 15 total; see CFWEP web page for pictures: www.cfwep.org o Highly recommended that all pictures be laminated; good alternative is to use sheet protectors 10 metal key rings Roll of string Hole puncher Assembly Instructions: Punch holes in pictures (Figure 7): o 4 holes for overstory picture (2 at top and 2 at bottom) o 4 holes for understory picture (2 at top and 2 at bottom) o 2 holes for ground cover picture (2 at top) Cut 20 pieces of string approximately 50 cm long 3 LESSON 4: Measuring Watershed Health Part II – Data Collection FIELDTRIP PRACTICE EXERCISES Tie string to bottom of overstory pictures and top of understory pictures Tie string to bottom of understory pictures and top of ground cover pictures (Figure 8) Insert the key rings to the top of the overstory picture (Figure 9) For some background information on the structural layers and biodiversity involved with riparian vegetation assessment, please see Background Information following water quality instructions. 4 LESSON 4: Measuring Watershed Health Part II – Data Collection FIELDTRIP PRACTICE EXERCISES Practice Water Quality Station Objectives: In this current lesson, which is also part of Lesson 4: Measuring Watershed Health Part II – Data Collection, students are given the opportunity to practice how to use the equipment used for the water quality station. Materials: Practice datasheet for water quality (Figure 10) GLX multi-meter (2-3) (Figure 11) Sets of 6 labeled cups for water samples (including rinse water cup; 2-3 sets; Figure 12) Table salt and small caps or cups (for distributing salt) Mine tailings Berkeley Pit water (if available) Plastic spoons Paper or cloth towels Masking tape (for labeling cups and station) Permanent marker Scissors Healthy parameters sheet (laminated) Definitions handout Instructions for Assembling GLX Multimeter Cans of pop (preferably dark-colored pop) 5 LESSON 4: Measuring Watershed Health Part II – Data Collection FIELDTRIP PRACTICE EXERCISES Procedure: Before students arrive, set up 2 to 3 water quality stations around the classroom, but not too far from each other. Each station should have: a GLX multimeter; 5 labeled cups for water samples; 1 labeled cup for rinse water; a cup with salt (Figure 11); a spoon; a cloth towel; the Healthy Parameters sheet; the Definitions handout; and the Instructions for Assembling GLX Multimeter. 5 cups should be labeled with masking tape and marker as follows: 1) tap water; 2) tap water & salt; 3) soda (or pop); 4) tap water & tailings; and 5) Berkeley Pit water. One more cup should be labeled as rinse water. Fill 3 of the water samples cups and the rinse water cup with tap water; fill 1 other cup with soda (pop); leave the last cup labeled pit water empty until further instructions. Label the station as Station 1, Station 2, etc (Figure 12). Notify the students that they are going to practice using the GLX which they will use in the field to determine water quality. Also inform students that the GLX is a piece of equipment used by actual scientists to study water quality. Lastly, caution students to be very careful with the equipment. In addition, warn students about the dangers of tailings and pit water. These items can pose health hazards and great care should be taken not to spill water samples or spread tailings. Instruct the students to follow the directions on the Instructions for Assembling GLX Multimeter and assemble the GLX’s. Next, instruct the students to read through the practice datasheet and follow instructions as written. Remind students that, as written in the directions, they MUST rinse each probe between each water sample measure in the rinse water cup. The students should record their results on the practice datasheet (a less dense version of the field datasheet). At this point inform students that they will only measure water pH, conductivity and temperature for practice. In the field, several more measures will be conducted and recorded. For some background information on the water quality parameters involved with water quality assessment, please see Background Information below. After both stations are completed by all student groups, go over data and discuss results (see lesson plan for Lesson 4). 6 LESSON 4: Measuring Watershed Health Part II – Data Collection FIELDTRIP PRACTICE EXERCISES BACKGROUND INFORMATION Vegetation Background: The Overstory (canopy) Layer1 The overstory (also called the canopy) is the highest vegetative layer. In CFWEP’s study, the overstory is defined as any vegetation over 30 meters tall. The primary vegetation found in the overstory is mature trees. The overstory is filled by leaves of mature trees. During the growing season, overstory leaves intercept much of the sunlight available to the lower layers. Typically less than 50% of the total amount of sunlight can pass through the overstory to plants in the lower layers. In a deciduous forest, the overstory is typically the last layer to show green in the spring. Since the overstory trees receive sunlight throughout the growing season, they can wait longer to deploy their leaves. This reduces the risk of the young tender leaves being destroyed by a late freeze. The Understory Layer1 Just beneath the overstory is the understory. In CFWEP’s study, the understory is defined as any vegetation between 0.5 and 30 meters tall. It is common to find other studies that split the understory layer into, sometimes up to 3 additional categories. The primary vegetation found in the understory includes tree saplings, small shade-tolerant trees, shrubs/bushes, and tall herbaceous plants. The understory can be thought of as a tree sapling staging ground. In a mature forest, many saplings can claim enough nutrients and sunlight to reach the understory. However, further growth is typically impractical as the saplings cannot obtain enough additional nutrients from established overstory trees to grow any higher. Therefore, many saplings slow their growth and wait in the understory until a mature overstory tree dies. How well a sapling can grow in full shade and how long a sapling can survive in the understory are two principle measures of a tree's shade tolerance. When a mature tree dies and opens a gap in the overstory, all of the saplings waiting in the understory rush upward. The saplings quick growth is fueled by the sudden increase in sunlight and nutrients no longer claimed by the deceased tree. The race to reach the overstory is very much a race for survival. There is typically only room for one new tree in the overstory. The tree that reaches the overstory continues to grow and expand, gradually reducing the flow of sunlight and nutrients to the trees below. All saplings that committed to the growth race, but failed to reach the overstory, gradually weaken and eventually die. The understory typically provides an abundance of food for animals such as deer and bears. In fact, many plants of the understory depend on wildlife to distribute their seeds. The animals ingest the plants' fleshy fruits and distribute the seeds in their feces. 7 LESSON 4: Measuring Watershed Health Part II – Data Collection FIELDTRIP PRACTICE EXERCISES The Ground Cover Layer1 The ground cover layer is the layer closest to the ground. In CFWEP’s study, ground cover is defined as any vegetation from the ground up to 0.5 meters tall. In addition, for our studies, ground cover also includes bare ground (includes dead plant matter), rocks, and tailings. The primary plants of the ground cover include tree seedlings, herbaceous plants, and grasses. Plants of the ground cover are typically the first plants to turn green in the spring. These plants have to deploy their leaves early in the growing season to capture direct sunlight to kick-start their growth cycle. Once the understory and overstory plants have deployed their leaves, very little sunlight remains for plants in the ground cover layer. Many plants in the ground cover layer have short life cycles. Since CFWEP’s curriculum is geared towards comparing unimpacted, less impacted or restored field sites to impacted field sites, we have also included the recording of other plants of the ground cover layer that reveal information regarding the health of the riparian vegetation. Specifically, we ask students to identify pollution-tolerant plants (tufted hairgrass and saltgrass), moisture-loving plants (sedges and rushes) and bare ground with tailings. Information on plant layers was modified from descriptions obtained from the following web page: http://www.mightytrees.com/science/foreststrat.html. 1 Water Quality Background: What is water quality? The term water quality is generally used to describe the chemical, physical and biological characteristics of water with respect to its suitability for its many particular uses. For example, water quality as it relates to drinking water has different requirements than water suitable for fish and other aquatic life, not to say that water safe for drinking will not support other uses. The point here is that there is a lot to consider when determining water quality. Even when looking at the data from scientific field monitoring, it is not as simple as saying “this water is good” and “this water is bad.” The quality of water we drink, and the quality of water in our rivers, streams, and lakes is important to all life. Water pollution can sometimes be detected visually or through an unusual odor. Often, the contaminants or pollutants cannot be detected by sight or smell, but they are there none-the-less. Even in small quantities, some of these substances can cause harm, or even be toxic to the living organisms that rely on the water. The effects of mining on water can be tested in rivers and streams many years after mining operations have ceased. In addition to practicing how to use the GLX multimeter, the exercise associated with this lesson also provides the opportunity to explain to students what pH and conductivity mean in relation to water. In addition, this exercise can demonstrate how tailings change water quality and why tailings are harmful for fish and bugs. The use of the temperature probe is simply to allow students to practice attaching and using the probe. 8 LESSON 4: Measuring Watershed Health Part II – Data Collection FIELDTRIP PRACTICE EXERCISES What does conductivity measure? Conductivity (also called specific conductance) is a measure of a material's ability to conduct an electric current. Specifically, conductivity measures the dissolved load – materials that cannot be seen in solution. The conductivity of a solution of water is highly dependent on its concentration of dissolved salts and sometimes other chemicals which tend to ionize in the solution. Electrical conductivity of water samples is used as an indicator of how salt-free or impurity-free the sample is – the purer the water, the lower the conductivity. Conductivity is used as a water quality indicator. Conductivity is measured in units called microSiemans per centimeter (µS/cm). The typical conductivity of Montana streams and rivers is about 50-500 µS/cm. Polluted freshwater typically has a conductivity above this range. Conductivity values are neither good nor bad. In other words, high conductivity does not necessarily indicate pollution. For example, ocean conductivity is very high due to salts and other minerals, and yet ocean waters support a great diversity and abundance of life. What is important with respect to conductivity and water quality is the nature of the dissolved material. In our watershed, high conductivity of fresh stream or river water may be indicative of dissolved heavy metals such as copper, zinc, and iron. These heavy metals are can cause aquatic life great harm. Common factors that affect conductivity include low flow conditions (less dilution of high salt content input sources), temperature and pH, especially if there are significant amounts of metals in the water. This latter phenomenon is manifested best by looking at acid rock drainage (ARD), which produces sulfuric acid through the oxidation of iron pyrite. Most metals’ solubility increases as the acidity of solution increases (as the pH decreases). What does pH measure? Two charged particles, H+ and OH-, are what make up pure water. Remember that water in the environment is never pure; it is an aqueous solution containing many dissolved salts and even gases. The pH of a solution is a measure of the amount or concentration of the H+ ions in a solution, which tells us if our water solution is an acid or a base. pH is measured in standard units on a logarithmic scale ranging from 0 to 14 (see figure at right). Logarithmic means that measurements on the scale increase or decrease by a power of 10. A pH of 4 is 100 times more acidic than a pH of 6, and 1,000 times more acidic than a pH of 7, and so on. Absolutely pure water, or a solution that is neither acidic or basic, measures at a pH of 7 (right in the middle of the scale). We call solutions with a pH of 7 neutral. If there are more H+ ions in solution than OH-, the pH will be less than 7, or acidic. Common examples of acidic solutions include vinegar and sulfuric acid (used in car batteries). If there are more OH- ions than H+, the solution will be above 7 or basic. Examples of 9 LESSON 4: Measuring Watershed Health Part II – Data Collection FIELDTRIP PRACTICE EXERCISES simple bases are sodium hydroxide (baking soda) and ammonia. Bases can be thought of as the chemical opposite of acids. A reaction between an acid and base is called neutralization. The pH of healthy, natural streams and rivers in Montana fall anywhere between 6.5 and 8.5, meaning they can be slightly acidic or basic, or near neutral (as these pH values are also called). Any stream or water source with a measurement outside of this range (either more acidic or basic) indicates that some human impact (e.g., pollution) or natural process is changing the chemical state of the water. For example, runoff from abandoned mines or mining wastes high in iron pyrite can significantly lower the pH of a stream. This process, facilitated by the oxidation of the pyrite which releases sulfuric acid into the environment, is known as acid rock (or mine) drainage (ARD). Conversely, streams that are fed by groundwater in limestone geology tend to have a higher or basic pH. This phenomenon is caused by the geologic dissolving of CaCO3 into water which results in formation of bicarbonate, a basic or alkaline substance. Temperature is another physical parameter of water that refers to how cold or warm the stream is. In water quality monitoring and scientific field research, temperature is measured in degrees Celsius. Celsius temperature data can be converted to Fahrenheit by multiplying the Celsius reading by 9/5 and adding 32. The majority of trout streams in Montana require a temperature of 15-19 °C or 60-65 °F. Recording temperature of a site’s stream water is important because dissolved oxygen levels (source of oxygen used by aquatic organisms) decrease as water warms up. Also, the growth rates of aquatic plant and animal life increase with a rise in temperature, which can lead to excessive algae growth and increased turbidity (cloudiness) of water. Some common phenomena that can influence water temperature include groundwater inflow, streamside vegetation (shading, or a lack of it) and water depth. 10