CURRICULUM VITAE – November 10, 2014
W. Matthew Michael
Contact Information
Molecular & Computational Biology
Department of Biological Sciences
University of Southern California
104B RRI
1050 Childs Way
Los Angeles, CA 90089
Telephone
Email
(213) 740-0553
mattm@usc.edu
Education
University of California, Berkeley
A.B. in Genetics, 1986
University of Pennsylvania
Ph.D. in Molecular Biology, 1996
Advisor: Professor Gideon Dreyfuss
Postdoctoral Training
1997 - 2000
University of California, San Diego
Advisor: Professor John Newport
Appointments
2010 Associate Professor
Department of Biological Sciences
College of Letters, Arts, and Sciences
University of Southern California
2004 - 2010
2000 - 2004
Associate Professor
Assistant Professor
Department of Molecular and Cellular Biology
Faculty of Arts and Sciences
Harvard University
Honors
1997 Damon Runyon-Walter Winchell Cancer Research Fund Postdoctoral Fellowship
2001 Searle Scholar Award
2002 National Science Foundation CAREER Award
2003 American Cancer Society Research Scholar Award
Matthew Michael
Publications
1. Drzislav Mismer, W. Matthew Michael, Todd R. Laverty and Gerald M. Rubin.
Analysis of the promoter of the Rh2 opsin gene in Drosphila melanogaster. Genetics
120: 173-180. 1988
2. W. Matthew Michael, David D. L. Bowtell, Todd R. Laverty and Gerald M. Rubin.
Comparison of the sevenless genes of Drosophila virilis and Drosophila melanogaster.
Proceedings of the National Academy of Sciences (USA) 87: 5351-5353. 1990
3. David D. L. Bowtell, Thomas Lila, W. Matthew Michael, David Hackett, and Gerald
M. Rubin. Analysis of the enhancer element that controls expression of sevenless in the
developing Drosophila eye. Proceedings of the National Academy of Sciences (USA)
88: 6853-6857. 1991
4. Michael J. Matunis, W. Matthew Michael and Gideon Dreyfuss. Characterization and
primary structure of the poly(C)-binding heterogeneous nuclear ribonucleoprotein
complex K protein. Molecular and Cellular Biology 12: 164-171. 1992
5. Andrea Ghetti, Serafín Piñol-Roma, W. Matthew Michael, Carlo Morandi and Gideon
Dreyfuss. hnRNP I, the polypyrimidine tract-binding protein: Distinct nuclear
Localization and association with hnRNAs. Nucleic Acids Research 20: 3671-3678.
1992
6. Haruhiko Siomi, Michael J. Matunis, W. Matthew Michael and Gideon Dreyfuss. The
pre-mRNA binding K protein contains a novel evolutionarily conserved motif. Nucleic
Acids Research 21: 1193-1198. 1993
7. Zhigang Weng, Sheila M. Thomas, Richard J. Rickles, Jennifer A. Taylor, Andrew W.
Brauer, Cynthia Seidel-Dugan, W. Matthew Michael, Gideon Dreyfuss, and Joan S.
Brugge. Identification of Src, Fyn, and Lyn SH3-binding proteins: Implications for a
function of SH3 domains. Molecular and Cellular Biology 14: 4509-4521. 1994
8. Xose R. Bustelo, K.L. Suen, W. Matthew Michael, Gideon Dreyfuss, and Mariano
Barbacid. Association of the Vav protooncogene with poly (rC)-specific RNA-binding
proteins. Molecular and Cellular Biology 15: 1324-1332. 1995
9. W. Matthew Michael, Mieyoung Choi, and Gideon Dreyfuss. A nuclear export signal
in hnRNP A1: A signal-mediated, temperature-dependent nuclear protein export
pathway. Cell 83: 415-422. 1995
10. W. Matthew Michael, Haruhiko Siomi, Mieyoung Choi, Serafín Piñol-Roma, Sara
Nakielny, Qing Liu, and Gideon Dreyfuss. Signal sequences which target nuclear import
and nuclear export of pre-mRNA binding proteins. Cold Spring Harbor Symposium of
Quantitative Biology 60: 663-668. 1995
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Matthew Michael
11. W. Matthew Michael and Gideon Dreyfuss. Distinct domains in ribosomal protein L5
mediate 5S rRNA binding and nucleolar localization. Journal of Biological Chemistry
271: 11571-11574. 1996
12. Utz Fischer, W. Matthew Michael, Reinhard Lührmann, and Gideon Dreyfuss.
Signal-mediated nuclear export pathways of proteins and RNAs. Trends In Cell Biology
6: 290-293. 1996
13. Victoria W. Pollard*, W. Matthew Michael*, Sara Nakielny, Mikiko C. Siomi, Fan
Wang, and Gideon Dreyfuss. A novel receptor-mediated nuclear protein import pathway.
Cell 86: 985-994. 1996 (* denotes equal contribution)
14. Sara Nakielny, Utz Fischer, W. Matthew Michael, and Gideon Dreyfuss. RNA
Transport. Annual Review of Neuroscience 20: 269-301. 1997
15. W. Matthew Michael, Paul S. Eder, and Gideon Dreyfuss. The K Nuclear Shuttling
Domain: Identification of a novel nuclear import and nuclear export signal in the hnRNP
K protein. EMBO Journal 16: 3587-3598. 1997
16. W. Matthew Michael and John Newport. Coupling of mitosis to the completion of S
phase through Cdc34-mediated degradation of Wee1. Science 282: 1886-1889. 1998
17. W. Matthew Michael. Two for the price of one: Nucleocytoplasmic shuttling signals.
Trends In Cell Biology 10: 46-50. 2000
18. W. Matthew Michael, Robert Ott, Ellen Fanning, and John Newport. Activation of
the DNA replication checkpoint through RNA synthesis by primase. Science 289: 21332137. 2000
19. W. Matthew Michael. Cell Cycle: Connecting DNA replication to sporulation in
Bacillus. Current Biology 11: R443-R445. 2001
20. Matthew P. Stokes, Ruth A. Van Hatten, Howard Lindsay, and W. Matthew Michael.
DNA replication is required for the checkpoint response to damaged DNA in Xenopus
egg extracts. The Journal of Cell Biology 158: 863-872. 2002
21. Ruth A. Van Hatten, Antonin Tutter, Antonia Holway, Alyssa Khederian, Johannes
Walter, and W. Matthew Michael. The Xenopus Mus101 protein is required for
recruitment of Cdc45 to origins of DNA replication. The Journal of Cell Biology 159:
541–547. 2002
22. Matthew P. Stokes and W. Matthew Michael. DNA damage-induced replication
arrest in Xenopus egg extracts. The Journal of Cell Biology 163: 245-255. 2003
23. Matthew P. Stokes and W. Matthew Michael. A novel replication arrest pathway in
response to DNA damage. Cell Cycle 3: 126-127. 2004
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Matthew Michael
24. Antonia H. Holway, Crystal Hung, and W. Matthew Michael. Systematic, RNAimediated identification of mus-101 modifier genes in C. elegans. Genetics 169: 14511460. 2005
25. Craig A. Leach and W. Matthew Michael. Ubiquitin/SUMO modification of PCNA
promotes replication fork progression in Xenopus laevis egg extracts. The Journal of Cell
Biology 171: 947-954. 2005
26. Antonia H. Holway, Seung-Hwan Kim, Adriana LaVolpe, and W. Matthew Michael.
Checkpoint silencing during the DNA damage response in C. elegans embryos. The
Journal of Cell Biology 172: 999-1008. 2006
27. Shan Yan, Howard D. Lindsay, and W. Matthew Michael. Direct requirement for
Xmus101 in ATR-mediated phosphorylation of Claspin-bound Chk1 during checkpoint
signaling. The Journal of Cell Biology 173: 181-193. 2006
28. Seung-Hwan Kim, Antonia H. Holway, Suzanne Wolff, Andrew Dillin, and W.
Matthew Michael. SMK-1/PPH-4.1 mediated silencing of the CHK-1 response to DNA
damage in early C. elegans embryos. The Journal of Cell Biology 179: 41-52. 2007
29. Seung-Hwan Kim and W. Matthew Michael. Regulated proteolysis of DNA
polymerase eta during the DNA damage response in C. elegans. Molecular Cell 32: 757766. 2008
30. Shan Yan and W. Matthew Michael. TopBP1 and DNA polymerase alpha directly
recruit the 9-1-1 complex to stalled replication forks. The Journal of Cell Biology 184:
793-804. 2009
31. Shan Yan and W. Matthew Michael. TopBP1 and DNA polymerase alpha-mediated
recruitment of the 9-1-1 complex to stalled replication forks: Implications for a
replication
restart-based mechanism for ATR checkpoint activation. Cell Cycle 8: 2877-2884. 2009
32. Ashley B. Williams and W. Matthew Michael. Eviction notice: new insights into
Rad51 removal from DNA during homologous recombination. Molecular Cell 37: 157158. 2010
33. Christopher Van, Shan Yan, W. Matthew Michael, Shou Waga, and Karlene A.
Cimprich. Continued primer synthesis at a stalled replication fork contributes to
checkpoint activation. The Journal of Cell Biology 189: 233-246. 2010
34. Christopher Murphy and W. Matthew Michael. Control of DNA replication by the
nucleus/cytoplasm ratio in Xenopus. Journal of Biological Chemistry 288: 29382-29393.
2013
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Matthew Michael
35. Liping Bai, W. Matthew Michael, and Shan Yan. Importin -dependent nuclear
import of TopBP1in ATR-Chk1 checkpoint in Xenopus egg extracts. Cellular Signaling
26: 857-867. 2014
36. Frances Tran and W. Matthew Michael. A cdc-25.1 allele that separates replication
checkpoint control from normal cell cycle timing in C. elegans. manuscript under review
(Genetics)
37. Melina Butuci, Ashley B. Williams, Matthew Wong, and W. Matthew Michael.
Zygotic genome activation triggers chromosome breaks in C. elegans primordial germ
cells. manuscript under review (Molecular Cell)
38. Julyana Acevedo, Shan Yan, and W. Matthew Michael. Biochemical mechanism for
TopBP1 recruitment to sites of DNA replication stress. manuscript in preparation
Grant Support
Current
National Institute of Health (NIGMS) #RO1 GM067735
2003 - 2015
Replication checkpoint activation and silencing
National Institute of Health (NIGMS) #RO1 GM099825
2011-2015
ATR-Chk1 signaling during embryonic and germ line development in C. elegans.
Completed
Searle Scholar Award #01-A-101
2001 - 2004
National Science Foundation #MCB 0133774 (CAREER Award)
2002 - 2007
American Cancer Society #RSG DDC-105870
2003 – 2008
National Institute of Health (NIGMS) #RO1 GM067735 – ARRA Administrative
Supplement 2009-2010
Invited Presentations
Meetings
American Association for Cancer Research Annual Meeting, New Orleans, March, 2001.
Eukaryotic DNA Replication, Cold Spring Harbor Laboratory, September, 2001.
The Cell Cycle, Cold Spring Harbor Laboratory, May, 2002.
DNA Repair and Mutagenesis, Bermuda, November, 2004.
DNA Replication and Genome Stability, Salk Institute, August, 2006.
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Matthew Michael
Eukaryotic DNA Replication, Cold Spring Harbor Laboratory, September, 2007.
The Cell Cycle, Cold Spring Harbor Laboratory, May, 2008.
American Society for Biochemistry and Molecular Biology, April, 2010.
Departmental Seminars
Department of Cancer Cell Biology, Harvard School of Public Health, April 1999.
Cancer Center, UC Davis School of Medicine, April 1999.
Department of Pharmacology, Columbia University, August 1999.
Tularik, Inc., September, 1999.
Department of Molecular Biology, UT Southwestern Medical Center, January 2000.
Department of Biological Sciences, Vanderbilt University, January 2000.
Department of Chemistry and Biochemistry, University of Colorado, Boulder, February,
2000.
Department of Molecular and Cellular Biology, Harvard University, February, 2000.
Department of Biomolecular Chemistry, University of Wisconsin, February, 2000.
Cancer Center, University of Michigan School of Medicine, February, 2000.
Department of Cell Biology, Yale University School of Medicine, March, 2000.
Abramson Cancer Research Institute, University of Pennsylvania, March, 2000.
Department of Pharmacology, University of Washington,, March, 2000.
Department of Physiology, UC San Francisco, April, 2000.
Cancer Center, Massachusetts General Hospital, October 2000.
Department of Biological Sciences, Vanderbilt University, November, 2002.
The Burnham Cancer Institute, La Jolla, January, 2003
Department of Molecular Oncology, Genentech, October 2003
Department of Biological Sciences, Vanderbilt University, January 2004
Department of Molecular Oncology, Genentech, June 2004
Huntsman Cancer Institute, University of Utah, October 2004
Department of Biology, Tufts University, October 2004
Department of Biochemistry and Molecular Biology, Johns Hopkins University, February
2006
Department of Cell Biology, Emory University Medical School, January 2007
Department of Pharmacology, Dartmouth Medical School, April 2007
Molecular Genetics Seminar Series, Brandeis University, October 2007
Department of Biochemistry, University of Wisconsin, Madison, December 2007
Department of Genetics and Genomics, Boston University, January 2008
Department of Molecular and Cellular Biology, Harvard University, November, 2008
Department of Microbiology and Molecular Genetics, University of Pittsburgh Medical
School, May, 2009
Department of Radiation Oncology, Dana-Farber Cancer Institute, July, 2009
Department of Cancer Biology, Scripps Research Institute (Florida), August, 2009
Institute of Cellular and Molecular Biology, University of Texas, Austin, September,
2009
Department of Biological Sciences, University of Southern California, October, 2009
Department of Biochemistry, Vanderbilt School of Medicine, November, 2009
Molecular Oncology Research Institute, Tufts Medical Center, December, 2009
Molecular Genetics Seminar Series, Brandeis University, April 2010
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Matthew Michael
Institute for Research in Immunology and Cancer, University of Montreal, December
2010
Department of Molecular and Cellular Biology, UC Davis, February, 2013
Training record
Student
Antonia Holway
Matthew P. Stokes
Craig A. Leach
Rajiv Gangurde
Laura L. Corey
Eric Perkins
Angela Holmes
Seung-Hwan Kim
Shan Yan
Ashley Williams
Christopher Murphy
Position
Graduate Student
Graduate Student
NRSA Postdoctoral Fellow
Postdoctoral Fellow
NRSA Postdoctoral Fellow
NRSA Postdoctoral Fellow
Postdoctoral Fellow
Postdoctoral Fellow
Postdoctoral Fellow
Postdoctoral Fellow
Graduate Student
Current students
Julyana Acevedo
Melina Butuci
Frances Tran
Matthew Wong
Hovik Gasparyan
Graduate Student
Graduate Student
Graduate Student
Graduate Student
Postdoctoral Fellow
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Current position
industry (Aushon)
industry (Cell Signaling Technology)
industry (Life Sensors)
industry (Blue Sky)
Assistant Professor, Illinois College
industry (Adgene)
industry (Adgene)
student, Divinity School
Assistant Professor, UNC-Charlotte
Postdoctoral Fellow, Cologne
Postdoctoral Fellow, UNC