Dr. rer. nat. Peter Fischer 1 ____________________________________________________________________________________________ CURRICULUM VITAE – Peter Fischer, Ph.D. DATE OFFICE ADDRESS: January 5, 2006 Washington University School of Medicine, Infectious Diseases Division, 660 S. Euclid, Campus Box 8051 St. Louis Mo 63110; Phone: +1-314-4547876, E-mail: Pufische@im.wustl.edu DEGREES: Habilitation and Venia Legendi (Parasitology, “Privatdozent”), 2004, research report: “Studies on the control of human pathogenic filarial infections”, Faculty of Medicine, University of Hamburg (Germany), Ph.D. (Zoology, “Dr. rer. nat.”), 1996, thesis: “Parasitological, molecular biological and epidemiological studies on the filarial parasite Onchocerca volvulus in Uganda”, Faculty of Biology, University of Hamburg (Germany), Master of Science (Biology, “Diplombiologe”), 1991, Major subjects: ecology, zoology, parasitology, thesis: “Reservoir competence of European vertebrates for the tick-borne agent of Lyme disease”, Faculty of Biology, Free University of Berlin (Germany). POSTS HELD: Since February 2005, Visiting Associate Professor, Infectious Diseases Division, Washington University School of Medicine, St. Louis, MO, USA 1998-2005, Research Scientist and laboratory group leader filariasis research, Bernhard Nocht Institute for Tropical Medicine (BNI), Hamburg, Germany 1997-1998, Postdoctoral fellow, Department of Biological Sciences, Smith College, Northampton, MA, USA 1996-1997, Postdoctoral fellow, Department of Helminthology, BNI 1993-1996, Research assistant and doctoral student, Department of Helminthology, BNI 1991-1992, Research assistant, Institute of Pathology, Free University of Berlin 1989-1991, Laboratory instructor, Institute of Zoology, Free University of Berlin GRANTS AND SCHOLARSHIPS: 2005-2007, Grant support evaluation and monitoring of Brugia timori control on Alor island, Indonesia (grant extension), GlaxoSmithKline 2002-2004, Grant support evaluation and monitoring of Brugia timori control on Alor island, Indonesia, GlaxoSmithKline 2002-2003, Habilitation scholarship, “Vereinigung der Freunde des Hamburger Tropeninstituts” 2001, Grant support filariasis diagnosis, UNDP/WorldBank/WHO-TDR 1998-2001, Group leader scholarship, Scholarship Program Infectiology (BMBF) 1997-1998, Research scholarship, German Academic Exchange, DAAD FIELD WORK: Three stays (4 months each) in Fort Portal, western Uganda (1993-1996); Short-term work (3-6 weeks): Alor island, Indonesia (four stays, 2001-2004), Dr. rer. nat. Peter Fischer 2 ____________________________________________________________________________________________ Department of Parasitology, University of Indonesia, Jakarta (1998) Research and Training Center for Vectors of Diseases, Ain Shams University, Cairo, Egypt (1998, 1999). MEMBERSHIP IN SCIENTIFIC ORGANIZATIONS: Deutsche Gesellschaft für Parasitologie (DGP), Deutsche Gesellschaft für Tropenmedizin und internationale Gesundheit (DTG), American Society of Tropical Medicine and Hygiene (ASTMH) PUBLICATIONS: (Original Papers in Peer Reviewed Journals) 53. Kraemling M, Bonow I, Schmetz C, Liebau E, Büttner DW, Fischer P (2006) Localization of an aspartate aminotransferase in the membranous organelles of spermatozoa from filarial parasites. Experimental Parasitology, submitted 52. Supali T, Ismid IS, Wibowo H, Djuardi Y, Majawati E, Ginanjar P, Fischer P (2006) Estimation of prevalence of lymphatic filariasis by a pool screen polymerase chain reaction assay using blood spots collected on filter paper. Transactions of the Royal Society of Tropical Medicine and Hygiene, in press 51. Jordanova R, Radoslavov G, Fischer P, Boteva R, Walter RD, Bankov I, Liebau E (2005) The highly abundant protein Ag-lbp55 from Ascaridia galli represents a novel type of lipid binding proteins. Journal of Biological Chemistry, 280: 41429-41438. 50. Oqueka T, Supali T, Ismid IS, Rückert P, Bradley M, Fischer P (2005) Impact of two rounds of mass drug administration using diethylcarbamazine combined with albendazole on the prevalence of Brugia timori and of intestinal helminths on Alor Island, Indonesia. Filaria Journal, 4:5. 49. Fischer P, Bonow I, Supali T, Rückert P, Rahmah N (2005) Detection of filaria-specific IgG4 antibodies and filarial DNA, for the screening of blood spots for Brugia timori. Annals of Tropical Medicine and Parasitology, 99:53-60. 48. Jordanova R, Radoslavov G, Fischer P, Liebau E, Walter RD, Bankov I, Boteva R (2005) Conformational and functional analysis of the lipid binding protein AgFABP from the parasitic nematode Ascaridia galli. The FEBS Journal, 272: 180-189. 47. Heukelbach J, Bonow I, Witt L, Feldmeier H, Fischer P (2004) High infection rate of Wolbachia endobacteria in the sand flea Tunga penetrans from Brazil. Acta Tropica, 92: 225-230. 46. Jolodar A, Fischer P, Büttner DW, Miller DJ, Schmetz C, Brattig NW (2004) Onchocerca volvulus: expression and immunolocalization of a nematode cathepsin Dlike lysosomal aspartic protease. Experimental Parasitology, 107: 145-156. 45. Supali T, Rahmah N, Djuardi Y, Sartono E, Rückert P, Fischer P (2004) Detection of filaria-specific IgG4 antibodies using the Brugia Rapid Test in individuals from an area highly endemic for Brugia timori. Acta Tropica, 90: 255-261. 44. Jolodar A, Fischer P, Büttner DW, Brattig NW (2004) The Wolbachia endosymbionts of Onchocerca volvulus express a putative secretory HtrA-type serine protease. Microbes and Infection, 6: 141-149. 43. Helmy H, Fischer P, Farid HA, Bradley MH, Ramzy RMR (2004) Test-strip detection of Wuchereria bancrofti amplified DNA in wild-caught Culex pipiens and estimation of infection rate by a poolscreen algorithm. Tropical Medicine and International Health, 9: 158-163. Dr. rer. nat. Peter Fischer 3 ____________________________________________________________________________________________ 42. Fischer P, Djuardi Y, Ismid IS, Rückert P, Bradley M, Supali T (2003) Long-lasting reduction of Brugia timori microfilariae following a single dose of diethylcarbamazine combined with albendazole. Transactions of the Royal Society of Tropical Medicine and Hygiene, 97: 446-448. 41. Rathaur S, Fischer P, Domagalski M, Walter RD, Liebau E (2003) Brugia malayi and Wuchereria bancrofti: gene comparison and recombinant expression of -class related glutathione S-transferases. Experimental Parasitology, 103: 177-181. 40. Jolodar A, Fischer P, Bergmann S, Büttner DW, Hammerschmidt S, Brattig NW (2003) Molecular cloning of an α-enolase from the human filarial parasite Onchocerca volvulus that binds human plasminogen. Biochimica et Biophysica Acta, 1627: 111-120. 39. Büttner DW, Wanji S, Bazzocchi C, Bain O, Fischer P (2003) Obligatory symbiotic Wolbachia endobacteria are absent from Loa loa. Filaria Journal, 2:10 38. Sommer A, Rickert R, Fischer P, Steinhart H, Walter RD, Liebau E (2003) A dominant role of the extracellular glutathione S-transferase from Onchocerca volvulus (Ov-GST1) is the production of prostaglandin D2. Infection and Immunity, 71: 3603-3606. 37. Fischer P, Djoha S, Büttner DW, Zipfel P (2003) Isolation and characterization of the regulatory subunit of cAMP-dependent protein kinase from the filarial parasite Onchocerca volvulus. Molecular and Biochemical Parasitology, 128: 33-42. 36. Fischer P, Bonow I, Büttner DW, Kamal IH, Liebau E (2003) An aspartate aminotransferase of Wolbachia endobacteria of Onchocerca volvulus is recognized by IgG1 antibodies of residents from endemic areas. Parasitology Research, 90: 38-47. 35. Fischer P, Schmetz C, Bandi C, Bonow I, Mand S, Fischer K, Büttner DW (2002) Tunga penetrans: molecular identification of Wolbachia endobacteria and their recognition by antibodies against proteins of endobacteria from filarial parasites. Experimental Parasitology, 102: 201-211. 34. Williams SA, Laney SJ, Bierwert LA, Saunders LJ, Boakye DA, Fischer P, Goodman D, Helmy H, Hoti SL, Lammie PJ, Plichart C, Ramzy RM, Ottesen EA (2002) The mosquito PCR diagnostic project: to develop a rapid assessment tool for the detection of Wuchereria bancrofti infected mosquitoes. Annals of Tropical Medicine and Parasitology, 96 (Suppl. 2): S41-46. 33. Fischer P, Wibowo H, Pischke S, Rückert P, Liebau E, Ismid IS, Supali T (2002) PCRbased detection and identification of the filarial parasite Brugia timori from Alor island, Indonesia. Annals of Tropical Medicine and Parasitology, 96: 809-821. 32. Supali T, Ismid IS, Rückert P, Fischer P (2002) Treatment of Brugia timori and Wuchereria bancrofti infections in Indonesia using DEC or a combination of DEC and abendazole: adverse reactions and short-term effects on microfilariae. Tropical Medicine and International Health, 7: 894-901. 31. Supali T, Wibowo H, Rückert P, Fischer K, Ismid IS, Purnomo, Djuardi Y, Fischer P (2002) High prevalence of Brugia timori infection in the highland of Alor island, Indonesia. American Journal of Tropical Medicine and Hygiene, 66: 560-565. 30. Pischke S, Büttner DW, Liebau E, Fischer P (2002) An internal control for the detection of Onchocerca volvulus DNA by PCR-ELISA and rapid detection of specific PCR products by DNA Detection Test Strips™. Tropical Medicine and International Health, 7: 526-531. 29. Kamal IH, Fischer P, Adly M, El Sayed AS, Morsy ZS, Ramzy RMR (2001) Evaluation of a PCR-ELISA to detect Wuchereria bancrofti in Culex pipiens from an Egyptian village with low prevalence of filariasis. Annals of Tropical Medicine and Parasitology, 95: 833-841. 28. Sommer A, Nimtz M, Conradt H, Brattig N, Boettcher K, Fischer P, Walter RD, Liebau E (2001) Structural analysis and antibody response to the extracellular glutathione Stransferases from Onchocerca volvulus. Infection and Immunity, 69: 7718-7728. Dr. rer. nat. Peter Fischer 4 ____________________________________________________________________________________________ 27. Krause S, Sommer A, Fischer P, Brophy PM, Walter RD, Liebau E (2001) Gene structure of the extracellular glutathione S-transferase from Onchocerca volvulus and its overexpression and promoter analysis in transgenic Caenorhabditis elegans. Molecular and Biochemical Parasitology, 117: 145-154. 26. Klüber S, Supali T, Williams SA, Liebau E, Fischer P (2001) Rapid PCR-based detection of Brugia malayi DNA from blood spots by DNA Detection Test Strips™. Transactions of the Royal Society of Tropical Medicine and Hygiene, 95: 169-170. 25. Sommer A, Fischer P, Krause K, Boettcher K, Brophy PM, Walter RD, Liebau E (2001) A stress responsive glyoxalase I from the parasitic nematode Onchocerca volvulus. Biochemical Journal, 353: 445-452. 24. Bockarie MJ, Fischer P, Williams SA, Zimmerman P, Griffin L, Alpers MP, Kazura JW (2000) Application of a polymerase chain reaction-ELISA to detect Wuchereria bancrofti in pools of wild-caught Anopheles punctulatus in a filariasis control area in Papua New Guinea. American Journal of Tropical Medicine and Hygiene, 62: 363-367. 23. Liebau E, Eschbach ML, Tawe W, Sommer A, Fischer P, Walter RD, Henkle-Dührsen K (2000) Identification of a stress-responsive theta-class Onchocerca volvulus glutathione S-transferase (Ov-GST-3) by RT-PCR differential display. Molecular and Biochemical Parasitology, 109: 101-110. 22. Fischer P, Supali T, Wibowo H, Bonow I, Williams SA (2000) Detection of DNA of nocturnally periodic Brugia malayi in night and day blood samples by a polymerase chain reaction-ELISA based method using an internal control DNA. American Journal of Tropical Medicine and Hygiene, 62: 291-296. 21. Fischer P, Tukesiga E, Büttner DW (1999) Long-term suppression of Mansonella streptocerca microfilariae following treatment with ivermectin. Journal of Infectious Diseases 180: 1403-1405. 20. Fischer P, Liu X, Lizotte-Waniewski M, Kamal IH, Ramzy RMR, Williams SA (1999) Development of a quantitative, competitive polymerase chainreaction-enzyme-linked immunosorbent assay for the detection of Wuchereria bancrofti DNA. Parasitology Research 85: 176-183. 19. Fischer P, Büttner DW, Bamuhiiga J, Williams SA (1998) Detection of the filarial parasite Mansonella streptocerca in skin biopsies by a nested polymerase chain reaction based assay. American Journal of Tropical Medicine and Hygiene 58: 816-820. 18. Fischer P, Yocha J, Rubaale T, Garms R (1997) PCR and DNA hybridization indicate the absence of animal filariae from vectors of Onchocerca volvulus in Uganda. Journal of Parasitology 83: 1030-1034. 17. Fischer P, Garms R, Büttner DW, Kipp W, Bamuhiiga J, Yocha J (1997) Reduced prevalence of onchocerciasis in Uganda following either deforestation or vector control with DTT. East African Medical Journal 74: 321-326. 16. Fischer P, Bamuhiiga J, Büttner DW (1997) Occurrence and diagnosis of Mansonella streptocerca in Uganda. Acta Tropica 63: 43-55. 15. Wildenburg G, Plenge-Bönig A, Renz A, Fischer P, Büttner DW (1997) Distribution of mast cells and their correlation to inflammatory cells around Onchocerca gutturosa, O. tarsicola, O. ochengi and O. flexuosa. Parasitology Research 83: 109-120. 14. Fischer P, Bamuhiiga J, Büttner DW (1997) Treatment of human Mansonella streptocerca infection with ivermectin. Tropical Medicine and International Health 2: 191-199. 13. Fischer P, Rubaale T, Meredith SEO, Büttner DW (1996) Sensitivity of a PCR-based assay to detect Onchocerca volvulus DNA in skin biopsies. Parasitology Research 82: 395-401. 12. Fischer P, Bamuhiiga J, Kilian ADH, Büttner DW (1996) Strain differentiation of Onchocerca volvulus from Uganda using DNA probes. Parasitology 112: 401-408. Dr. rer. nat. Peter Fischer 5 ____________________________________________________________________________________________ 11. Fischer P, Kilian ADH, Bamuhiiga J, Kipp W, Büttner DW (1996) Prevalence of Mansonella perstans in western Uganda and its detection using the QBC®-fluorescence method. Applied Parasitology 37: 32-37. 10. Kipp W, Kabwa P, Verbeck A, Fischer P, Eggert P, Büttner DW (1995) Prevalence and risk factors of HIV-1 infection in three parishes in western Uganda. Tropical Medicine and Parasitology 46: 141-146. 9. Fischer P, Kipp W, Kabwa P, Büttner DW (1995) Onchocerciasis and HIV in western Uganda: Prevalences and treatment with ivermectin. American Journal of Tropical Medicine and Hygiene 53: 171-178. 8. Fischer P, Kipp W, Bamuhiga J, Binta-Kahwa J, Kiefer A, Büttner DW (1993) Parasitological and clinical characterization of Simulium neavei-transmitted onchocerciasis in western Uganda. Tropical Medicine and Parasitology 44: 311-321. 7. Matuschka F-R, Heiler M, Eiffert H, Fischer P, Lotter H, Spielman A (1993) Diversionary role of hoofed game in the transmission of Lyme disease spirochetes. American Journal of Tropical Medicine and Hygiene 48: 693-699. 6. Matuschka F-R, Fischer P, Heiler M, Blümcke S, Spielman A (1992) Stage-associated risk of transmission of the Lyme disease spirochete by European Ixodes ticks. Parasitology Research 78: 695-698. 5. Matuschka F-R, Fischer P, Heiler M, Richter D, Spielman A (1992) Capacity of European animals as reservoir hosts for the Lyme disease spirochete. Journal of Infectious Diseases 165: 479-483. 4. Matuschka F-R, Fischer P, Musgrave K, Richter D, Spielman A (1991) Hosts on which nymphal Ixodes ricinus most abundantly feed. American Journal of Tropical Medicine and Hygiene 44: 100-107. 3. Matuschka F-R, Richter D, Fischer P, Spielman A (1990) Nocturnal detachment of the tick Ixodes hexagonus from nocturnally active hosts. Medical and Veterinary Entomology 4: 415-420. 2. Matuschka F-R, Richter D, Fischer P, Spielman A (1990) Time of repletion of subadult Ixodes ricinus ticks feeding on diverse hosts. Parasitology Research 76: 540-544. 1. Matuschka F-R, Lange R, Spielman A, Richter D, Fischer P (1990) Subadult Ixodes ricinus (Acari: Ixodidae) on rodents in Berlin, West Germany. Journal of Medical Entomology 27: 385-390. (Review articles) IV. Michael E, Malecela-Lazaro MN, Maegga BTA, Fischer P, Kazura JW (2006) Mathematical models and lymphatic filariasis control II. Monitoring and evaluation of interventions. Trends in Parasitology, submitted III. Fischer P, Supali T, Maizels RM (2004) Lymphatic filariasis and Brugia timori: prospects for elimination. Trends in Parasitology, 20: 351-355. II. Fischer P, Boakye D, Hamburger J (2003) Polymerase chain reaction-based detection of lymphatic filariasis. Medical Microbiology and Immunology, 192: 3-7. I. Fischer P, Büttner DW (2002) The epidemiology of onchocerciasis and the long term impact of existing control strategies on this infection. World Class Parasites Vol. 5: The Filaria, Klei T, Rajan TV ed., Kluwer Academic Publishers, Boston, Dordrecht, London, pp: 43-57. (Book review) Fischer P (2002) Lymphatic filariasis. Book review. Trends in Parasitology, 18: 48. Dr. rer. nat. Peter Fischer 6 ____________________________________________________________________________________________ (Abstracts of talks and posters since 2000) 40. Fischer P, Ingram J, Buttner DW, Schmetz C, Slatko B (2005) A Wolbachia endosymbiont DNA sequence is horizontally transferred to the nuclear genome of the filarial parasite Brugia malayi. 53nd Annual Meeting of the American Society of Tropical Medicine and Hygiene in Washington, American Journal of Tropical Medicine and Hygiene 73 (Suppl), 57 39. Rao R, Weil GJ, Atkinson LJ, Fischer K, Supali T, Fischer P (2005) A practical realtime PCR based assay for detecting parasite DNA in blood from Brugia infected individuals. 53nd Annual Meeting of the American Society of Tropical Medicine and Hygiene in Washington, American Journal of Tropical Medicine and Hygiene 73 (Suppl), 757 38. Borchert N, Wagner A, Fischer P, Brattig NW (2005) Tissue degrading enzymes of filariae. 8. Kongress für Infektionskrankheiten und Tropenmedizin. Hamburg. 37. Fischer P, Oqueka T, Rückert P, Ismid IS, Manoempil P, Supali T (2004) Characterization and control of lymphatic filariasis due to Brugia timori on Alor island, Indonesia. 1st Asean Congress of Parasitology and Tropical Medicine, Kuala Lumpur, Malaysia. 36. Supali T, Rahmah N, Djuardi Y, Wibowo H, Ismid IS, Sartono E, Rückert P, Fischer P (2004) Detection of anti-filarial IgG4 using the Brugia Rapid test in individuals from areas endemic for Brugia malayi and Brugia timori. 1st Asean Congress of Parasitology and Tropical Medicine, Kuala Lumpur, Malaysia. 35. Fischer P, Jolodar A, Bonow I, Bandi C, Brattig NW, Büttner DW (2003) Crossreactivity of antibodies to antigens of Wolbachia from filarial nematodes with filarial antigens or with Wolbachia-antigens from arthropod parasites. 52nd Annual Meeting of the American Society of Tropical Medicine and Hygiene in Philadelphia, Pennsylvania, American Journal of Tropical Medicine and Hygiene 69 (Suppl), 461. 34. Rückert P, Krentel A, Supali T, Fischer P, Manoempil P, Ismid I, Servais G, Setyawati I, Kielmann T (2003) Lymphatic filariasis control on Alor island, Indonesia: experiences leading to the change of the national policy. GTZ - Summer Seminar, Glashütten, Germany. 33. Fischer P, Supali T, Krentel A, Rückert P, Kielmann T, Bradley M, Manoempil P, Rahmah N, Ismid IS (2003) Control of filariasis on Alor Island, Indonesia: from basic research to the revision of the national control policy. Joint Meeting of the Deutsche Gesellschaft für Technische Zusammenarbeit (GTZ), Deutsche Gesellschaft für Tropenmedizin und Internationale Gesundheit, WHO-TDR, Berlin. 32. Fischer P, Schmetz C, Feldmeier H, Bandi C, Heukelbach J, Bonow I, Büttner DW (2003) Wolbachia endobacteria in the sand flea Tunga penetrans. 7. Kongress für Infektionskrankheiten und Tropenmedizin, Berlin. Infection 31 (Suppl.), 66. 31. Fischer P, Rückert P, Wibowo H, Rahmah N, Ismid IS, Supali T (2002) Brugia timori infection on Alor island, Indonesia: prevalence, diagnostics and control. 51st Annual Meeting of the American Society of Tropical Medicine and Hygiene in Denver, Colorado, American Journal of Tropical Medicine and Hygiene 67 (Suppl.), 143. 30. Pischke S, Klüber S, Supali T, Rückert P, Bonow I, Fischer P (2002) PCR-based detection of Brugia timori from Alor island, Indonesia. 20. Tagung der Deutschen Gesellschaft für Parasitologie, Lübeck-Travemünde, Germany. 29. Wagner A, Fischer P, Jolodar A, Haffner A, Brattig NW (2002) Characterization of tissue-degrading secretory metallo- and serine proteases of the filarial tissue parasite Onchocerca volvulus. 20. Tagung der Deutschen Gesellschaft für Parasitologie, Lübeck-Travemünde, Germany. Dr. rer. nat. Peter Fischer 7 ____________________________________________________________________________________________ 28. Fischer P, Bonow I, Büttner DW, Kamal IH, Liebau E (2001) IgG1 antibodies of residents from filariasis endemic areas recognize an aspartate aminotransferase of Wolbachia endobacteria. 50th Annual Meeting of The American Society of Tropical Medicine and Hygiene in Atlanta, American Journal of Tropical Medicine and Hygiene 65 (Suppl.), 1966. 27. Abbasi I, Koech DK, Supali T, Fischer P, Hamburger J (2001) Sputum-PCR for diagnosis of lymphatic filariasis: from genomics to field studies. Conference on filariasis, Hamburg, Germany. 26. Fischer P, Klüber S, Pischke S, Bonow I, Supali T (2001) PCR-based diagnosis of lymphatic filariasis and onchocerciasis. Conference on filariasis, Hamburg, Germany. 25. Supali T, Wibowo H, Rückert P, Fischer K, Ismid IS, Purnomo, Djuardi Y, Fischer P (2001) High prevalence of Brugia timori infection in the highland of Alor island, Indonesia. Conference on filariasis, Hamburg, Germany. 24. Klüber S, Supali T, Wibowo H, Bonow I, Williams SA, Fischer P (2000) Detection of Brugia malayi DNA in blood spot samples from Indonesia by PCR-ELISA. 49th Annual Meeting of The American Society of Tropical Medicine and Hygiene in Washington, American Journal of Tropical Medicine and Hygiene 63 (Suppl.), 589. 23. Fischer P, Klüber S, Supali T, Bockarie M, Williams SA (2000) DNA diagnostics of lymphatic filariasis. European filariasis conference, Hamburg, Germany. 22. Fischer P, Liebau E, Bonow I, Sommer A, Williams SA, Kamal IH (2000) Survey for expressed sequence tags in female Onchocerca volvulus following treatment with ivermectin. Meeting on protective immunity in onchocerciasis, Edna McConnell Clark Foundation, Woods Hole, USA. 21. Fischer P, Supali T, Wibowo H, Bonow I, Williams SA (2000) Detection of Brugia malayi DNA in day and night blood samples by a PCR-ELISA method using an internal control DNA. 19. Tagung der Deutschen Gesellschaft für Parasitologie, StuttgartHohenheim, Germany.