INSTITUTIONAL ANIMAL ETHICS COMMITTEE
SUMMARY SHEET
(TO BE FILLED BY INVESTIGATOR)
1.
Type of Animals used (Please Tick)
requested
Number of Animals
a. Mice
b. Rats
c. Rabbits
√
12
d. Hamster
e. Primates
f. Others
2.
Clearance nature recommended (Please Tick)
1. Routine
(Sacrificing for tissue and no extended treatmentinjections or surgical)
2. Other
√
a. Non invasive procedures
b. Surgical procedures
b. Treatment with agents
i)
Infectious
ii)
Non infectious
iii)
No treatment
√
3.
Whether Biosafety (BPL3) facilities/clearance needed: No
4.
Comments/Remarks: (To be filled by IAEC-member)
Investigator signature
IAEC-MEMBER Signature
BRIEF SUMMARY OF THE PROJECT
(ONE PARAGRAPH IN LAYMAN’S TERM)
One of the aims of the project is to study the molecular basis of insecticide
resistance in indian malaria vector Anopheles stephensi which involves
molecular characterization of knockdown resistance and major genes involved
in metabolization of insecticides. We are also trying to understand the
association of various novel knockdown resistance (kdr) mutations with
phenotypic resistance. To understand the possible molecular basis of insecticide
resistance we are selecting lines of An. stephensi with different insecticide
susceptibility in order to do transcriptome analysis. We are also maintaining
lines of An. stephensi with different genotypes responsible for insecticide
resistance.
Form B (per rule 8(a)
NIMR/IAEC2015
APPLICATION FOR PERMISSION FOR ANIMAL EXPERIMENTS
Application to be submitted to sent either to the CPCSEA (address in form A
above) or Institutional Animal Ethics Committee (IAEC)
Part A
1* Name and address of establishment
National Institute of Malaria Research (ICMR),
Sector-8, Dwarka, New Delhi-11077
2* Registration
number
33/ReBi/GO/99/CPCSEA
3
and
date
of
registration:
Name, address and registration number of breeder from which animals
acquired (or to be acquired) for experiments mentioned in parts B & C
Animal
Facility
NIMR,
33/ReBi/GO/99/CPCSEA
Sector-8,
Dwarka,
New
Delhi,
4
Place where the animals are presently kept (or proposed to be kept)
Animal Facility NIMR, Sector-8, Dwarka, New Delhi,
5
Place where the experiment is to be performed (Please provide CPCEA
Reg. Number) Insectary at NIMR
6
Date on which the experiment is to commence and duration of
experiment
To be an ongoing regular facility for blood feeding of mosquitoes
7
Type of research involved (Basic Research/Educational/Regulatory)
Basic Research
Signature
OP Singh
Name and designation of Investigator
Date: 25-08-2015
Place: Delhi
*Applicable only for application to be submitted to CPCSEA
PART B
Protocol form for research proposals to be submitted to the
committee/Institutional
Animal
Ethics
Committee,
for
new
experiments or extensions of ongoing experiments using animals other
than non-human primates.
1. Project / Dissertation / Thesis Title: Centre for the studies on complex
malaria
2. Principal Investigator / Research Scholar / Research Guide /Advisor :
a. Name: Dr. O.P. Singh
b. Designation: Scientist F
c. Dept / Div/ Lab: Molecular Biology Division
d. Telephone No.: 011-25307323
e. Experience: 29 years
3. List of names of all individuals authorized to conduct procedures under
this proposal
Sh. Bhopal Ram Arya- Field worker
Sh. Sadrrudin- TS
Sh. Ramesh- Technician A
Mrs. Taranjeet Kaur- SRF
4. Funding source: NIH, USA
5. Duration of the project: 7 years (2011-17)
a. Number of months: Regular ongoing activity
b. Date of initiation (Proposed): Aug 2012
c. Date of completion (Proposed): July 2017
6. Detailed study plan may be given (Not more than one page)
Study Plan
We have currently following strains of mosquotoes in my laboratory
1. An. stephensi—DDT resistant (selection process ongoing)
2. An. stephensi—deltamethrin resistant (selection process ongoing)
3. An. stephensi—strain with genotype L1014S (kdr)
4. An. stephensi—strain with genotype L1014 (wild)
5. An. stephensi—Alwar strain
6. An. stephensi—Jodhpur strain
Mosquito colony maintenance
Adult female mosquitoes will be generally be offered 10% glucose for survival
but for egg laying mosquitoes will be fed on rabbit host. Rabbit will be
placed in a small cage and which will be placed in mosquito cage containing
adult mosquito (50 nos.) for one hour duration. After one hour of offering
blood meal, rabbit will be freed and will be kept in animal house.
The mosquitoes are being maintained in NIMR insectary since more than
one year and are free from any infection.
7. Animals required
a. Species/Common name: Rabbits
b. Age / weight / size: Adult Rabbit (>2Kg)
c. Gender: Either Sex
d. Number to be used (Year-wise breakups and total figures needed
to be given): Year 2015-16: 12
Year 2016-17: 12
Total:
24 rabbits
e. Number of days each animal will be housed. Entire Life
f. Proposed source of animals: Animal facility, NIMR, Dwarka, New
Delhi
8. Rationale for animal usage
a. Why is animal usage necessary for these studies?
 For feeding experiment with the laboratory colonized An. stephensi
with different insecticide susceptibility mosquitoes as blood meal
from animal is necessary to complete the life cycle of mosquitoes.
b. Why are the particular species selected required?
 Blood meal from the rabbit is most suitable for regular colony
maintenance due to ease of handling and maintenance.
c. Why is the estimated number of animals essential?
 Nature of mosquito feeding behavior is complex and random.
Especially which is very fragile and its culture in insectory is
challenging. If lost it will be very difficult to revive selected strains
and thus special attention to meet and maintain regular blood
feeding demands uninterrupted for each cyclic colony. Therefore a
minimum number of animals are proposed to work without the
scarcity of samples needed for experiments.
d. Are similar experiments conducted in the past? If so, the number of
animals used and results obtained in brief.
 No
e. If yes, why new experiment is required?
 NA
f. Have similar experiments been made by any other organization
agency? If so, their results in your knowledge.
 Various mosquito species/strains are maintained worldwide by this
procedure.
9. Description the procedures to be used.

Animal Procurement: Required animal would be either procures
from NIMR, animal facility or from other registered organization.
The proposed study does not require any surgical manipulation of
the animal. The animals would be used only for the mosquito blood
feeding experiments. For proper animal maintenance, animal
facility would be utilized and veterinary service/care would be
provided to the animals as and when required. Carcass of dead
animal would be disposed of as per standard guidelines.
10. Please provide brief descriptions of similar studies from invitro/invivo
(from other animal models) on same/similar test component or line of
research. If, enough information is available, justify the proposed reasons.
NA
11. Does the protocol prohibit use of anesthetic or analgesic for the conduct
of painful procedures (any which cause more pain than that associated with
routine injection or blood withdrawal)? If Yes, explanation and justification
NO
12. Will survival surgery be done? NO
If Yes, the following to be described.
a. List and description of all such surgical procedures (including
methods of asepsis) NA
b. Names, qualifications and experience levels of operators NA
c. Description of post-operative care NA
d. Justification if major survival surgery is to be performed more than
once on a single individual animal. NA
13. Methods of disposal post-experimentation
a. Euthanasia (Specific method): NA
 We will not perform experiments on animals; naturally dead animal
will be stored at -200C till the time authorized biohazard waste
collection company person come to collect.
 Company Name: M/s SMS Water Grace BMW Pvt. Ltd. D.J.B,
S.T.P., NILOTHI, New Delhi-110041
b. Method of carcass disposal:
 Proper disposal of carcasses after natural death will be
undertaken by an assigned government approved agency (M/s
SMS Water Grace BMW Pvt. Ltd. D.J.B, S.T.P., NILOTHI, New
Delhi-110041)
c. Rehabilitation
: NA
14. Animal transportation methods if extra-institutional transport is
envisaged: NA
15. Use of hazardous agents (use of recombinant DNA-based agents or
potential human pathogens requires documented approval of the
Institutional Biosafety Committee (IBC). For each category, the agents and
the biosafety level required, appropriate therapeutic measures and the mode
of disposal of contaminated food, animal wastes and carcasses must be
identified) NA
(a) Radionuclides: NA
(b) Microorganisms / Biological infectious Agents: NA
(c) Hazardous chemicals or drugs: NA
(d) Recombinant DNA: NA
(e) Any other (give name): NA
If, your project involved use of any of the above, attach copy of the
minutes of IBC granting approval. : NA
Investigator's declaration
1. I certify that I have determined that the research proposal herein is
not unnecessarily duplicative of previously reported research.
2. I certify that, I am qualified and have experience in the
experimentation on animals.
3. For procedures listed under item 11, I certify that I have reviewed the
pertinent scientific literature and have found no valid alternative to
any procedure described herein which may cause less pain or distress.
4. I will obtain approval from the IAEC/CPCSEA before initiating any
significant changes in this study.
5. Certified that performance of experiment will be initiated only upon
review and approval of scientific intent by appropriate expert body
(Institutional Scientific Advisory Committee / funding agency / other
body (to be named).
6. Institutional Biosafety Committee's (IBC) certification of review and
concurrence will be taken (Required for studies utilizing DNA agents
of human pathogens).
7. I shall maintain all the records as per format (Form D)
8. I certify that, I will not initiate the study unless approval from CPCSEA
received in wiring. Further, I certify that I will follow the
recommendations of CPCSEA.
9. I certify that I will ensure the rehabilitation policies are adopted.
Signature
Date: 25-08-2015
O.P. Singh
Name of Investigator