DAR Request to Inject Cell Lines into Animals Form*
*This form must be approved by the GSU Attending Veterinarian prior to the use of said cell lines in
animals.
Please submit completed forms to the Associate to the Director) (mdavis1978@gsu.edu)
Submission Date: _____________
PI Name
Lab Contact
PI E-mail
Lab Contact E-mail
PI Phone Number
Lab Contact Phone Number
Speedtype to be Charged (if testing is determined to be necessary)
The injection of transplantable tumors, hybridomas, cultured cell lines, or other
biological materials into rodents can pose a health risk to animals and personnel.
These biological materials have been a source of mouse hepatitis virus, mousepox, and
other significant disease agents at research facilities. Moreover, rodent pathogens can
be carried and propagated by non-rodent (e.g. human) cell lines when these cell lines
have been propagated in rodents or rodent biological materials.
Consistent with GSU IACUC Policy, rodent cell lines (as well as non-rodent cell lines
propagated in rodents or using rodent biological materials) must be evaluated for rodent
pathogenic microorganisms by polymerase chain reaction (PCR) prior to their being
injected into an animal. Approval for the use of such cell lines in animals housed at GSU
will only be given after the Attending Veterinarian has assessed the test results to
determine their adequacy. Some vendors of cell lines will already have screened the
cells in which case additional testing may not be necessary.
Should testing be necessary, the PCR assay requires 2 vials of each sample with a
minimum of 1 x 107 cells/vial and a turnaround time of 7-10 days. Please fill out the
table below for each cell line you plan to use in animals. The cost of this testing will be
paid by the Principal Investigator.
Cell Line Name
Vendor
Cell Line # (if such
designation exists)
Do you have record of this cell
line having been previously
tested for rodent pathogens? (Y
or N) If Yes, please provide test
results.
Please note that individual cell lines must be approved for in vivo use by specific IACUC-approved
protocols.
_______________________________ _________________
University Veterinarian Signature
Date