2015 Experimental Molecular Biophysics 實驗分子生物物理學 Place: Rm102 of the Institute of Biological Chemistry, AS Time: 2:00 pm ~ 5:00 pm on Tuesdays TA: Hsin-Hung Lin 林新閎 Credit: 3 credits Perspective: The purpose of this class is to introduce different biophysical techniques and their possible applications. We will address how to combine different techniques in tackling specific biological issues. Grading scheme: Assignment/quiz: 70% Final presentation on the selected paper: 30% *Topics and the order of presentation will be decided by drawing lots on 3/24. Attendance: Unexplained absence: -3 of final grade (did not send notice to the TA or the programme secretary before class) Late: -2 of final grade Absence without supporting document:-1 of final grade (if a student did not inform the course TA or the programme secretary, Ms Elve Lin (elvelin@gate.sinica.edu.tw) to excuse him/herself from the class before lecture starts, he/she has to provide a proof to Ms Lin to explain his/her absence to the class.) Date Topic Instructor 02/24 ---Introduction--- Dr. Joseph Huang NMR ( assignment or quiz ) 03/03 03/10 03/17 03/24 Application of NMR spectroscopy in structural biology a. history and overview b. structural determination using solution NMR c. case studies Application of NMR spectroscopy in membrane protein research a. solution NMR approach b. solid-state NMR approach c. case studies Application of NMR spectroscopy in drug screening a. conventional methods b. case studies To decide presentation topics and orders Dr. Tsyr-Yan (Dharma) Yu Dr. Tsyr-Yan (Dharma) Yu Dr. Tsyr-Yan (Dharma) Yu Imaging (assignment or quiz) 03/31 Light Microscopy Dr. Keng-Hui Lin 04/07 Single Molecule Detection Dr. Keng-Hui Lin 04/14 Single Molecule Manipulation Dr. Keng-Hui Lin X-ray (assignment or quiz) 04/21 Protein Crystallization and Crystal Symmetry Dr. Hanna S. Yuan 04/28 X-ray Diffraction Basics Dr. Hanna S. Yuan 05/05 Structure Determination and Refinement Dr. Hanna S. Yuan Computational biophysics (assignment or quiz) 05/12 QM/MM simulations Dr. Carmay Lim 05/19 Docking and structure-based drug design Dr. Carmay Lim Cryo-EM (assignment or quiz) 05/26 Application of electron microscopy in structural biology Dr. Chi-Yu Fu 06/02 Basics of single particle EM and image processing Dr. Chi-Yu Fu 06/09 Application of single particle EM and image processing Dr. Chi-Yu Fu 06/16 -----Final Exam Week (oral presentation )---- 06/23 -----Final Exam Week (oral presentation)---