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Scientist
Member Number: 3971891
Albany, CA 94706
Portfolio: http://www.beyond.com/people/3971891
Professional Information
Job Function: Science & Biotech, Research
Education: Post Graduate Degree
Employment: Full-Time
Salary: $80-90k
Citizenship: U.S. Citizen
Resume
PERSONAL
Citizenship: US citizen
Languages: English, Russian
OBJECTIVE
A Mid to Senior level position in Research and Development.
SUMMARY
Ph.D. Biologist with significant academic and industry experience. Extensive theoretical and hands-on expertise in
Molecular and Cell Biology methods, Immunoassays and Biochemistry with emphasis on cloning and analysis of novel
genes, gene regulation and genetic/protein engineering. Experienced in the development of cell-based functional assays
and assays for selection of proteins from combinatorial libraries. Additional background in bioinformatics and
programming. Good analytical skills and management experience. Self starter. Able to work independently and in a teamoriented environment.
EDUCATION
***, Ph.D., Genetics. N. Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow.
Scientist
***, M.S., Physics. Moscow Engineering Physical Institute, Moscow.
RESEARCH EXPERIENCE
4/*** - present
Scientist II at Sangamo BioSciences, Inc., Richmond, CA. Genetic and protein engineering. Development of assays for
protein selections from combinatorial libraries in vivo (hybrid methods) and in vitro (displays). Design and functional
evaluation of engineered transcription factors, zinc finger proteins. Protein transduction studies. Computational analysis of
targeted genes. Regulation and analysis of gene expression in mammalian cells. Development of cell-based functional
assays.
4/*** - 3/***
Postdoctoral Research Associate with Dr. R. L. Juliano, Department of Pharmacology, University of North Carolina,
Chapel Hill, NC. Design of artificial, zinc finger based, transcription factors that selectively regulate the expression of
cancer related genes.
Development of yeast one-hybrid system for selection of DNA-binding zinc finger proteins by screening peptide
combinatorial libraries.
9/***
Postdoctoral Research Associate with Dr. H. B. Pakrasi, Department of Biology, Washington University, St. Louis, MO.
Biochemical analysis of photosystem II and photosystem I, membrane protein complexes; study of manganese transport
in cyanobacteria, structural and functional analysis of ABC transporter system for manganese. Cloning and molecular
analysis of novel genes related to photosynthesis.
***/***
Graduate student in the Laboratory of Genetics of Phototropic Microorganisms, Head - Dr. S. V. Shestakov, N. Vavilov
Institute of General Genetics, Moscow, Russia. Cloning and molecular analysis of cyanobacterial herbicide resistance
genes.
3/***
Research associate at the Department of Biophysics, Head - Dr. E. A. Krasavin, Joint Institute for Nuclear Research,
Dubna, Russia. Study of DNA-repair system induced by ionization radiation.
TECHNICAL SKILLS
Molecular Biology Methods:
- DNA, RNA isolation, purification, analysis;
- cloning, including cDNA cloninig;
- construction and screening of plasmid and retroviral libraries;
- sequencing;
- PCR, RT-PCR;
Scientist
- real-time quantitative PCR (TaqMan);
- hybridization (Southern, Northern), probe design;
- immunoblot (Western blot) analysis;
- immunoprecipitation;
- immunostaining;
- fluorescent microscopy;
- ELISA;
- site-directed mutagenesis;
- plasmid construction for expression in bacterial, yeast and mammalian cells;
- recombinant protein expression, purification, analysis;
- in vitro transcription/translation.
Assays for protein screening from combinatorial libraries:
- yeast one-hybrid assay;
- mammalian one-hybrid assay;
- bacterial two-hybrid assay;
- phage display;
- CIS display.
Cell Biology Methods:
- propagation of different mammalian cell types, including mouse embryonic stem cells;
- transient and stable transfections;
- retroviral vector delivery;
- protein transduction;
- reporter assays;
- flow cytometry (FACS).
Computer skills:
- proficient with standard protein and nucleotide sequence analysis tools and software (including BLAST, VectorNTI
Suite, GCG, Sequencher, Clone Manager);
- public database search;
- proficient with PC, MAC and UNIX environments;
- general software: Word, EXCEL, PowerPoint.
Programming Skills:
- Visual Basic;
- HTML.
LIST OF PUBLICATIONS
Scientist
Reik A., Zhou Y., Collingwood T.N., *** V.V., Kong Y., Henning K.A., Fallentine B.K., Zhang L., Zhong H., Jouvenot Y.,
Jamieson A.C., Rebar R.J., Case C.C., Korman A., Li X***Y., Black A., King D.J. and Gregory P.D. (***) Enhanced protein
production in mammalian cells using engineered zinc finger protein transcription factors. Nature Biotech., submitted.
Fields P.E., Lee G.R., Kim S.T., *** V.V., Flavell R.A. (***) Th***specific chromatin remodeling and enhancer activity in the
Th2 cytokine locus control region. Immunity, ***, ***
***, VV, Miller, J, Case, CC, Pabo, OC (***) Engineered zinc finger proteins for controlling stem cell fate. Stem Cells, ***,
***
*** V.V., Juliano R.L. (***) Regulation of the MDR1 gene by transcriptional repressors selected using peptide
combinatorial libraries. Mol. Pharmacol., ***, ***
Chandler L.E., *** V.V., Pakrasi H.B. (***) Regulation of manganese uptake in Synechocystis *** by RfrA, a member of a
novel family of proteins containing a repeated five-residues domain. Biochemistry, ***, ***
*** V.V., Pakrasi H.B. (***) Membrane topology of MntB, the transmembrane protein component of an ABC transporter
system for manganese in the cyanobacterium Synechocystis sp. Strain PCC *** J. Bacteriol., ***, ***
*** V.V., Pakrasi H.B. (***) Molecular identification of a novel protein that regulates biogenesis of Photosystem I, a
membrane protein complex. J. Biol. Chem., ***, ***
*** V.V., Pakrasi H.B. (***) Manganese transport in the cyanobacterium Synechocystis sp. PCC *** J. Biol. Chem., ***, ***
*** V.V., Pakrasi H.B. (***) Molecular identification of an ABC transporter complex for manganese: analysis of a
cyanobacterial mutant strain impaired in the photosynthetic oxygen evolution process. EMBO J., ***: ***
*** V. V., Shestakov S. V. (***) The dspA gene product of the cyanobacterium Synechocystis sp. strain PCC *** influences
sensitivity to chemically different growth inhibitors and has amino acid similarity to histidine protein kinases. Microbiology,
***, ***
Chesnavichene E. A., Elanskaya I. V., *** V. V., Shestakov S. V. (***) Molecular analysis of a new gene controlling
resistance of the cyanobacterium Synechocystis *** to metronidazole and nitrophenolic herbicides. Doklady Biological
Sciences, ***: ***
Shestakov S. V., *** V. V. (***) The molecular basis of resistance to the herbicide difunon in the cyanobacterium
Synechocystis sp. PCC *** Biologicheskie Nauki, 2 : *** (in Russian).
*** V. V., Amirtaev K. G., Krasavin E. A., Bonev M. N. (***) The modifying effect of glycerine and cysteamine on prophage
lambda induction in E. coli cells under gamma irradiation. Radiobiologiia, ***:*** (in Russian).
Bonev M. N., *** V. V. (***) Influence of the E. coli repair system on the spontaneous prophage lambda induction. JINR
Communication, E***, Dubna.
Scientist
ABSTRACTS
*** V.V., Reik A., Zhou Y., Kong M., Li X***Y., Warfe L., Zhang L., Case C.C., Collingwood T., Gregory P.D., Rebar E.J.
(***) Superactivation of the SRá and CMV Promoters Using Designed Zinc Finger Proteins. In: ASGT 8th annual Meeting.
St. Louis, USA. Abstract ***
*** V.V., Reik A., Zhou Y., Kong M., Li X***Y., Warfe L., Zhang L., Case C.C., Collingwood T., Gregory P.D., Rebar E.J.
(***) Superactivation of the SRÀ and CMV Promoters Using Designed Zinc Finger Proteins. In: ASGT 8th annual Meeting.
St. Louis, USA. Abstract ***
Urnov F., Holmes M., Gregory P., Jouvenot Y., *** V., Miller J. (***) Genetic and epigenetic manipulation of stem cell fate.
In: ISSCR 2nd Annual Meeting. Boston, MA, USA: ***
*** V.V., Miller J.C., Case C.C., Pabo C.O. (***) Controlling stem cell differentiation with designed zinc finger protein
transcription factors. In: ISSCR 2nd Annual Meeting. Boston, MA, USA: ***
Guschin D., Zhang S., Zhang L., Tan S., *** V., Case C., Rebar E., Gregory P. (***) Engineered Zinc Finger Transcription
Factors: A Platform for Therapeutic Gene Regulation with Exquisite Specificity. In: ASGT 7th annual Meeting.
Minneapolis, USA. Abstract ***
*** V.V., Miller J.C., Case C.C., Pabo C.O. (***) A Novel Approach to Stem Cell Gene Therapy: Controlling Stem Cell
Differentiation with Engineered Zinc-Finger Protein Transcription Factors. In: ASGT 7th annual Meeting. Minneapolis,
USA. Abstract ***
Xu D., *** V.V., Fisher M., Ye D., Juliano R.L. (***) Inducible expression of a selective repressor of the MDR1 multi-drug
resistance gene. In: Molecular Targets and Cancer Therapeutics: Discovery, Biology, and Clinical Applications. Miami
Beach, FL, USA: ***
Falke D., *** V., Fisher M., Juliano R.L. (***) Design of artificial transcription factors to selectively regulate the proapoptotic bax gene. In: Molecular Targets and Cancer Therapeutics: Discovery, Biology, and Clinical Applications. Miami
Beach, FL, USA: ***
*** V.V., Juliano R.L. (***) Genetically engineered repressors of the MDR1 gene promoter. In: Molecular Targets and
Cancer Therapeutics: Discovery, Development and Clinical Validation. Washington, DC, USA: ***
*** V.V., Labesse G., Mathews F.S., Pakrasi H.B. (***) Manganese transport in cyanobacteria. In: IX International
Symposium on Phototrophic Prokaryotes, Vienna, Austria: ***
Pakrasi H.B., *** V.V., Maitra R., Zak E. (***) Proteins that regulate biogenesis of pigment-protein complexes in thylakoid
membranes of cyanobacteria. In: IX International Symposium on Phototrophic Prokaryotes, Vienna, Austria: ***
Pakrasi H.B., Anbudurai P.R., *** V.V., Periyanan. S., Shukla V.K., Wu R. (***) Use of mutants to study the structure and
function of the photosystem II reaction center complex in Synechocystis *** In: VIII International Symposium on
Phototrophic Prokaryotes, Urbino, Italy: ***
Scientist
Shestakov S.V., *** V.V., Elanskaya I., Chesnavichene E., Sidoruk K. (***) Cloning and molecular analysis of genes
involved in the resistance to some herbicides in the cyanobacterium Synechocystis sp. PCC *** In: VIII International
Symposium on Phototrophic Prokaryotes, Urbino, Italy: ***
Shestakov S. V., Chesnavichene E. A., *** V. V., Elanskaya I. V. (***) Cloning and molecular analysis of genes controlling
resistance to herbicides in the cyanobacterium Synechocystis *** In: Workshop on Plant Mol. Biol. and Biotechnology,
Moscow, Russia: ***
REFERENCES
Under request