HORIZONTAL Factsheet
GERMINATING WEEDS
version: 2/12/2016
SUBJECT
Germinating weeds
HORIZONTAL number
8-2
Matrices
Various substrates
Existing standards used as basis
ISO/CD 17126 (draft), ÖNORM S 2021
Standardisation Committees
CEN TC 223, ISO TC 190
Work package
4
Project
HORIZONTAL
Expert
Andreas Baumgarten
Organisation
Agency for Health and Food Safety, Vienna
Country
Austria
Email
Status
andreas.baumgarten@ages.at
Desk study First
Comments Phase I
Final version
Phase II Ruggedness
Biological parameters
July 2003
December 2003
Ongoing work
www.ecn.nl/horizontal
EIONET
Completion due by 30-10-05
Second consultation
Completion due by 31-01-06
Validation
Completion due by 31-08-06
Final consultation
Completion due by 31-10-06
Transfer to BT/TF151
Completion due by 30-11-06
Summary of GERMINATING WEEDS procedure
Exposition of substrate under analysis to standardized environmental conditions, monitoring
of the germinating weeds
HORIZONTAL Factsheet
GERMINATING WEEDS
version: 2/12/2016
Placement of GERMINATING WEEDS method in overall structure HORIZONTAL
SLUDGE, SOIL, TREATED BIOWASTE AND RELATED
WASTES
SAMPLING
IN-SITU ,TRANSPORT, STORAGE
INORGANIC
CONSTITUENTS
PHYSICAL
PROPERTIES
SAMPLE PRETREATMENT
- HYG
SAMPLE PRETREATMENT - ORG & BIOL
SAMPLE PRETREATMENT - INORG
LEACHING
ORGANIC
PARAMETERS
HYGIENIC
PARAMETERS
BIOLOGICAL
PARAMETERS
PAH
pH
Characterisation
leaching tests
Bulk density
Impurities (e.g
glass, plastic)
E. Coli
Stability (bio)
Salmonella
Germinating
weed seeds
Clostridium
Phytotoxicity
Enterococci
AOX
Electrical
conductivity
Compliance
leaching tests
Dry matter
PCB
Organic matter*
Solidity
LAS, NP,NPE
Nutrients N, P,
K*
Thixotropic
behaviour
DEPH, DBP
Trace elements
solid *
Trace elements
determination*
Piling
behaviour
Helminth ova
BFR
Rapid methods
Flowability
PCCD/PCDF
Soluble P
Virusses and
bacteriophages
Pharmaceuticals
Plant
Pathogens
Position of GERMINATING WEEDS method in the sequence of steps from sampling to
reporting
Sampling
plan
Taking of
sample
Transport &
storage
Preparation of Extraction/
test portion
Leaching
Pre-treatment
Detection &
Summary
Quantification, reports
Enumeration
x
x
x
x
1)Sample conservation, storage and transport are issues that generally are covered in as the final step in sampling standards
2) Preparation of the test portion from a laboratory sample is sometimes part of the next step (digestion or analysis preparation) or can
be covered in a separate standard
3) Summary of test results is a concise summary of the relevant data for the end-user, which can be merged in an overall test report
covering all aspects relevant for the given situation.
4) After defining sampling strategy based on the question to be answered either one of the sampling standards will generally apply. All
the following steps will be the same for either route.
5) It is at present not clear where extraction of organic parameters begins and pre-treatment ends. It seems that pre-treatment now includes
the extraction step.
Major issues of discussion
In the course of the ruggedness test following points will be clarified:
dilution material, EC threshold, pH, nutrient status, Additional treatments, moisture, Thickness
of the layer, Size of the tray, duration
Impurities
Stability
weed
Bulk
Germinating
Piling
Solidity
Thixotropic
behaviour
Flowability
leaching
Compliance
Characterisation
glass,
Organic
pH
Dry
PAH
AOX
PCB
conductivity
Electrical
DEPH,
BFR
Nutrients
PCCD/PCDF
LAS,
K*
PE
Trace
determination*
solid
E.
Salmonella
Clostridium
Coli
matter
density
NP,N
*seeds
elements
plastic)
DBP
(bio)
tests
N,
(e.g
P,
SAMPLE
SITU
,TRANSPORT,
PHYSICAL
PROPERTIES
LEACHING
INORGANIC
CONSTITUENTS
ORGANIC
HYGIENIC
BIOLOGICAL
PARAMETERS
SAMPLING
-IN
ORG
&matter*
BIOL
SLUDGE,
SOIL,
WASTES
PRETREATMENT
STORAGE
TREATED
BIOWASTE AND
RELATED
HORIZONTAL Factsheet
GERMINATING WEEDS
version: 2/12/2016