PROTEIN PURIFICATION SYSTEM
MANUFACTURER: AMERSHAM BIOSCIENCES (GE)
MODEL: ÄKTA Purifier 10
APPLICATION: High resolution chromatography device for proteins, peptides and
nucleic acids purification. This device includes a series of prepacked columns for the
different types of chromatography.
INA ÄKTA Purifier 10 core system + Accessories.
ÄKTApurifier 10 core system.
TECHNICAL SPECIFICATIONS:
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Purification at µg-mg scale.
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10 ml Pump & Base provides a liquid flow of 0.001 to10 ml/min.
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Loop capacity: 10- 150 ml.
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Equipped with Monitor UV-900 which allows multiple UV-wavelength
monitoring of up to three wavelengths simultaneously.
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Monitor pH/C-900 expands ÄKTApurifier 10 and 100 systems to allow
measurement of conductivity, pH, and temperature. Both pH and
conductivity/temperature flow cells feature low cell volume and high linearity for
accurate measurements.
ÄKTA PURIFICATION SYSTEM CONFIGURATION
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BufferPrep Kit eliminates time-consuming manual buffer preparation and
titration usually needed for pH changes in chromatography. For any pH and salt
concentration entered, BufferPrep automatically calculates the composition of
the buffer online. BufferPrep enables automatic buffer titration and eliminates
errors occurring during manual preparation.
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ÄKTA design automation kits: ÄKTApurifier systems eliminate much of the
tedious and time-consuming manual steps involved in sample application by
bringing a greater degree of automation to the system’s setup.
Examples of additional functionality that can be applied to ÄKTApurifier core systems
using ÄKTAdesign automation kits.
Our device was updated with the following automation kits:
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Air sensor Kit, which is controlled by UNICORN software, detects air in
the system, and pauses the purification run. The kit therefore prevents
column damage caused by air and secures that the sample is
completely injected.
Buffer selection Kit manages buffer reagents effectively, allowing buffer
screening and minimizing the risk of air entering the system. The kit
enables automatic selection among up to eight different buffers to allow
optimized purification. Buffer selection Kit eliminates manual changing of
buffers and moving inlet tubing, ensuring that no air enters the system.
Column screening Kit provides the necessary motor valves to allow
automated method optimization, multistep purification, and easier
column selection. Up to eight columns can be connected to the system
in one go. Column performance can be easily compared while changing
conditions in a run. Preset protocols prevent system overpressurization
and prevent column breakage during use. Connecting two columns to
one of the motor valves included in the kit enables automated column
selection for optimizing both column and media performance, as well as
column switching for two-step purification protocols.
Reverse flow Kit contains motor valves for automated reverse flow
through a column. Strongly bound target proteins elute more effectively,
speeding up your purification. Resulting peaks are sharper and more
concentrated target protein is recovered. The kit increases the life
expectancy of your columns since reversing the flow efficiently rinses
impurity away from the top of the column.
Fractionation Kit enables collection of large volumes of purified sample.
Up to eight vessels of any size can be used to collect fractions.
Collection in large vessels rather than smaller vessels minimizes the risk
of losing valuable target protein, improving protein recovery and giving
you greater piece-of-mind.
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Fraction Collectors: Frac-920 allows collection of up to 175 fractions in 12mm diameter tubes, up to 95fractions in 10–18 mm tubes, as well as 40
fractions in 30-mm diameter tubes.
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Chromatography columns:
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Analytical:
 Mini Q 4.6/50 PE
0,8ml Minispheres IEX Strong anion
(NH4+).
 Mini S 4.6/50 PE 0,8ml Minispheres IEX Strong cation (methyl
sulphide).
 SOURCE 15RPC ST 4.6/100 (PE/divinilbenzene) 2ml Reverse
phase.
 RESOURCE RPC (polieteretercetona)
1ml Reverse phase.
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Preparative:
 Mono Q 10/100 GL 8ml sphereIEX Strong anion.
 HiLoad 16/10 Phenyl Sepharose HP 20ml HIC (high m).
 HiPrep 26/10 Desalting (Sephadex G-25)
53ml GF tampon
change (no salt containing).
 Superdex 200 10/300 GL 24ml GF proteins /DNA 104-5x10 D.
UNICORN control software permits methods edition, automatic control,
concentration and mg quantification, molecular mass determination and
recovery calculations. Designed for Windows 2000 and Windows XP,
UNICORN Control System software gives you real-time control of protein
purification systems.