1
CHE 552
BIOLOGICAL CHEMISTRY II (Spring 2004)
11:00am - 12:15pm TR (CP-222)
Instructor:
Dr. Stephen M. Testa
Office Hours: Tuesday 12:20-1:50 pm,
Thursday 12:20-1:50 pm
(or by appointment)
Office:
Phone:
E-mail:
CP-315
257-7076
testa@uky.edu
Textbook and Attendance: The textbook “Fundamentals of Biochemistry, Upgrade Edition”
by Voet, Voet, and Pratt (published by John Wiley & Sons, Inc) is required. Any other
sources that might be used will be detailed in the class notes. Since not all the material
covered can be found in a textbook, I highly recommend you attend class.
Grading Policy: Three exams will count toward your grade, each worth 25 points. Taking
the final exam is optional and can be used to replace your previous lowest test grade. The
remaining 25 points will come from a written report, which cannot be replaced.
The final grade will be determined as follows: The scores of the top 10% will be
averaged, anyone getting within 90% of this score will receive an A grade; within 80% a B
grade; within 70% a C grade; within 60% a D grade, less than 60% an F grade.
Differentiation of undergraduate and graduate students: Graduate and undergraduate
students, as required by the university, are held up to different standards in all 400G and 500
level courses. In this course, this rule manifests itself in the requirements for the written
report. Graduate student reports must be more in depth, longer, and contain more references.
Therefore, for undergraduates the report must consist of 1300 words and have at least 6
primary references. For graduate students the report must consist of 2000 words and have at
least 12 primary references.
Tentative Course Content:
Section #1
1/15
1/20
1/22
1/27
1/29
2/3
2/5
2/10
2/12
2/17
(Day 1):
(Day 2): (Day 3):
(Day 4): (Day 5):
(Day 6): (Day 7):
(Day 8): (Day 9):
Introduction to this course
Intro to nucleic acids / electrophoresis
DNA replication / restriction mapping
Transcription / reverse transcription
Translation / sequencing
Translation #2 / subcloning
Regulation / PCR
Discussion of reports, assign topics, help session for test
Test I
No Class
2
Section #2
2/19
2/24
2/26
3/2
3/4
3/9
3/11
(Day 10):
(Day 11): (Day 12):
(Day 13): (Day 14):
(Day 15): (Day 16):
Synthesis of nucleic acids / mutagenesis
Nucleic acid structure and stability / spectroscopy
Nucleic acid structure prediction
Nucleic acid structure-function relationships / hybridization
Paper #1 (Class Discussion) – Quiz #1 (applied to test grade, 10%)
help session for test
Test II
Section #3
3/23
3/25
3/30
4/1
4/6
4/8
4/13
4/15
4/20
4/22
(Day 17): (Day 18):
(Day 19): (Day 20):
(Day 21): (Day 22):
(Day 23): (Day 24):
(Day 25): (Day 26):
Chemical mapping / evolution
Viruses
Protein-nucleic acid interactions / SELEX / enzymes
Biotechnology (Class Discussion)
Paper #2 (Class Discussion) – Quiz #2 (applied to test grade, 10%)
Hand in reports, Paper #3 (Class Discussion) – Quiz #3 (10% of test)
Class discussion of reports
help session for test
Test III
TBA
Final Exam
5/4
(Day 27):
Final Exam (10:30 am)
Additional sources for course content and figures:
Biochemistry (4th ed): Stryer
Molecular Biology of the Cell (2nd ed): Alberts, Bray, Lewis, Raff, Roberts, and Watson
Biophysical Chemistry (Parts 1-3): Canter and Schimmel
The RNA World: Edited by Gesteland and Atkins
The RNA World (2nd ed): Edited by Gesteland, Cech, and Atkins
Nucleic Acids in Chemistry and Biology: Blackburn and Gait
Organic Chemistry: McMurry
Bioorganic Chemistry - Nucleic Acids: Hecht
Biochemistry and Molecular Biology: Elliot and Elliot
Genes V: Lewin
Nucleic Acids in Molecular Biology Series (Catalytic RNA): Edited by Eckstein and Lilley