UC Irvine Environmental Health & Safety: Example
Standard Operating Procedures for Hazardous Chemicals and Particularly Hazardous Substances
Hazardous Chemicals
#1 Process
Handling ENU in animals. Please ensure employees read the Material Safety Data Sheet at:
#2 Hazardous Chemicals/Class of Hazardous Chemicals
N- Nitroso –N ethylurea (synonym ENU). Staff must review the Material Safety Data Sheet prior to working with
any hazardous material. Protocol designed for liquid stock of ENU, not powder form.
#3 PPE - Personal protective Equipment
Use disposable nitrile gloves and a lab coat at all times when working with ENU.
#4 Engineering/Ventilation Controls
All procedures involving ENU must be handled inside the fumehood. Verify date of inspection of the fumehood on
the sticker. Fumehood must have been inspected within the lst year.
#5 Special Handling Procedures and Storage Requirements
1. In a chemical hood, measure the appropriate volume of ENU into a syringe. No handling of dry powders.
2.
Keep mice inside fume hood for at least 24 hours after exposure to ENU. When removing mice from fume
hood, transfer into clean cage and discard bedding as hazardous (solid) waste. The PI’s staff will change
contaminated bedding and place dirty bedding into a doubled plastic bag containing paper saturated with
inactivating solution. (see solution below), then sealed and placed in a rigid leak proof container labeled as
hazardous waste. Procedure will be conducted inside a fume hood.
3. Subsequent bedding changes may be disposed of as non-hazardous bedding waste.
#6 Spill and Accident Procedures
1. Clean all spills and soak all equipment, syringes and gloves coming into contact with ENU with the
inactivating solution and discard in a rigid leak proof container labeled as hazardous waste.
2.
Inactivate any remaining ENU on surfaces with 20-30 mls of inactivating solution. Let stand in the chemical
hood exposed to light overnight and discard in a rigid leak proof container labeled as hazardous waste.
3.
If nitrile gloves should become contaminated while working with ENU, apply inactivating solution on glove
and remove immediately and wash hands with soap and water.
4. Inactivating Solution: 20% w/v Na2S2O3 in 0.1N NaOH.
Waste Disposal
#7
Dispose of any liquid thiosulfate in a rigid leak proof container labeled as hazardous waste. Contact EH&S
for disposal as hazardous waste. (containers or pails may be obtained from physical sciences stores or a lab
safety supply vendor).
#8 Approval Required:
Approval by PI or Lab Supervisor required to perform protocol.
Reviewed by EH&S: S. Weekly, L. Cambria Campus Veterinarian G. Mulder
#9 Decontamination Procedures
Apply solution to all contaminated surfaces. See Item 6.
Formula for Deactivating solution:
50 gm Na2S203 (Sodium Thiosulfate)
1 gm NaOH
up to 250 mls with dH20
#10 Designated Area
Vivarium No. ________
For more information relevant to completing this form, review thoroughly Chemical Hygiene Plan: Section 5.
Training Acknowledgement: I have read, asked questions, and understand the hazards of and safe working
procedures for the activity/materials described herein.
NAME
DATE
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UC Irvine Environmental Health & Safety: Example
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