The Biopolymer/Genomics Core Facility
University of Maryland School of Medicine
Phone 410-706-8553; Fax 410-706-0287; E-mail biopolymer@som.umaryland.edu
DNA SEQUENCING ORDER FORM
CONTACT INFORMATION: (incomplete or illegible information may result in reporting delays)
Principal investigator (print last name, first name): ________________________________________________________
Signature of P.I. or authorized person: __________________________________________ Date:_______________
 UMGCC Member
PCBU & Project ID #’s: ______________________
 GCRC Funded Research
Department and School: _________________________________________________________________________
Name of Requester: (if other than P.I.)_______________________________________ Phone:
-
E-mail Address:
Please read and follow the detailed instructions on the back of the page.
**Templates and Primers must be IN WATER**
SERVICE REQUESTED (Check ONLY ONE box):
[] Run with Specified Primers ONLY (no additional runs, raw data returned)
[] Complete Single Stranded (assembled sequence returned as text file, additional primers/runs may be necessary)
[] Complete Double Stranded (assembled sequence returned as text file, submit 2 primers min., additional primers/runs may be necessary)
[] Dye Already Attached (submit in 96-well plate, see instructions on back of page)
[] Return Unused Samples/Primers
UNUSED SAMPLES/PRIMERS Will be Discarded in 5 business days, unless the above box is checked
If Samples/Primers are not picked up within 10 business days, they will be discarded
TEMPLATE INFORMATION (list all that apply, indicate which samples & primers should be used together):
[] ds Plasmid
[] PCR Product
[] Single stranded
[] High G/C content [] Other________
Template name: ______________________________________________________________
Insert or fragment size (required for Complete Single/Double) :
___________________________________
Volume(l) ______ Conc.( ng/uL) _______ OR A260____
A280____
Dilution____
Please make sure that all tubes are clearly marked with sample or primer name, date, & your initials!
PRIMER INFORMATION (check all that apply):
M13-Forward
T7
M13-Reverse
T7 Terminator
BGH Reverse
TKpolyA
Other BPGC Primer:________________
pGex5’
EGFP-N
CMV-Forward
pGex3’
EGFP-C
SP6
T3
Custom Primer (provide at 1M):______________
CMV Promoter
To Be Completed By BIOPOLYMER STAFF ONLY:
____ Full reactions at $8/run (we attach the dye) ________
____ Full reactions, 96 well trays $600/tray
________
____ Full 96 well trays, ready to run at $75/tray
________
____ Single runs, ready to run at $3.00/sample
________
____ Primers at $8.00/primer (for primer walking) ________
_______________________________________________________________________________
TOTAL
________
Biopolymer-Lab Sequence No.:
Form: DNA SEQ ORDER
Modification Date:01/09/2014
Invoiced By and Date:
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The Biopolymer/Genomics Core Facility
University of Maryland School of Medicine
Phone 410-706-8553; Fax 410-706-0287; E-mail biopolymer@som.umaryland.edu
DNA Sequencing Ordering Instructions
1.
Please note that failure to fill out the order form completely may result in delay of your order. Failure to
submit samples and/or primers in adequate quantity may result in poor quality data.
2.
Samples should be delivered to room HH560A.
3.
A minimum of 300ng plasmid DNA or 50ng PCR product is required for each run. A “run” equals one
sample tested with one primer (forward or reverse, not forward and reverse).
4.
Samples with high G/C content maybe difficult to sequence. Please check the box “high in G/C content” if
this applies to samples you submitted.
5.
Custom primers should be submitted at 1 M. A minimum of 3.5 L per run at 1 M is required.
6.
When submitting multiple sample and/or primer, list each on the form and indicate which samples and
primers should be used together. You may write in the margin if necessary.
7.
When submitting dye attached samples in a 96-well tray (plate):
A. Ensure that the tray is not warped. A warped tray can cause serious damage to the detection
instrument. The tray should lie flat when placed on the counter. If it does not lie flat, please
transfer samples to a new tray. Warping can be avoided by not exposing the tray to excessive heat
when drying down samples.
B. Place 25 L of sterile water in all unused wells of the 96-well tray. Empty wells may cause poor
quality data and/or damage to the detection instrument.
8.
Sequencing results will be returned to the email address provided on the order form.
9.
If Return Samples/Primers was requested, residual samples and/or primers may be picked-up at room
HH560A.
Form: DNA SEQ ORDER
Modification Date:01/09/2014
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