Supplementary Information for
The Protective Effects of Tryptophan on UV-induced DNA
Photodamage
Sulayman A. Oladepo and Glen R. Loppnow*
Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2 Canada
* Corresponding author: (voice): 780-492-9704; (fax): 780-492-8231; (e-mail):
glen.loppnow@ualberta.ca
ABSTRACT
This supplementary information contains supplementary data which are not included in the main
manuscript. The first is a Figure S1 which represents the heating and cooling curves for MBDNA hybrid in the presence and absence of Trp. Following, this are the individual plots of
normalized fluorescence vs. irradiation time for the different concentrations shown in Figure 4 of
the main manuscript. These are followed by the individual plots for the DNA-Trp sandwich
solutions irradiation experiments for DNA in the presence of outer layers of 0.1, 1 and 1.5 mM
Trp shown in Figure 5 of the main manuscript. The last figure represents DNA with 1.5 mM Trp
solution in which both species are present in the same medium.
S1
Figure S1: Heating (filled squares) and cooling (open squares) curves for 200 nM MB in the
presence of a 10-fold excess of target oligonucleotide and 5 M Trp (upper curves). Heating
(filled triangles) and cooling (open triangles) curves for 200 nM MB in the presence of a 10-fold
excess of target oligonucleotide (lower curves). Both pairs of curves were generated at a heating
rate of 1 oC/min, in 4 oC increments and 5 minutes holding time per increment. In both cases, the
melting temperature of the MB-DNA hybrid is essentially constant (melting temperature
difference is within experimental error) despite the presence of Trp. The fluorescence signal
levels are different because the fluorescence measurements were carried out in different
experiments, with different fluorescent lamp intensities.
Figure S2: Plot of normalized fluorescence intensity vs irradiation time for a solution of target
DNA containing no Trp. The plot is the same as that shown in Figure 4 of the main manuscript,
but is individually shown here for clarity. The fluorescence decay time constant, yo and A for this
DNA only sample are 1.2 ± 0.089 min, 0.1561 and 0.8431, respectively.
Figure S3: Same as Figure S2 but for DNA in the presence of 0.1 mM Trp. The fluorescence
decay time constant, yo and A for this sample are 2.3 ± 0.11 min, 0.1028 and 0.8883,
respectively.
Figure S4: Same as Figure S2 but for DNA in the presence of 1 mM Trp. The fluorescence
decay time constant, yo and A for this sample are 4.5 ± 0.42 min, 0.0677 and 0.9764,
respectively.
Figure S5: Same as Figure S2 but for DNA in the presence of 1.5 mM Trp. The fluorescence
decay constant, yo and A for this sample are 5.4 ± 0 min, 0.6536 and 0.2723, respectively. Error
bars representing the standard deviations are shown.
S2
Figure S6: Same as Figure S2 but for DNA in the presence of 5 mM Trp. The fluorescence
decay time constant, yo and A for this sample are 22 ± 3.7 min, 0.1093 and 0.8645, respectively.
Figure S7: Same as Figure S2 but for DNA in the presence of 10 mM Trp. The fluorescence
decay time constant, yo and A for this sample are 29 ± 2.8 min, 0.0960 and 0.8526, respectively.
Figure S8: Plot of normalized fluorescence intensity vs irradiation time for a solution of target
DNA solution immersed in a cuvette of 0.1 mM Trp. The fluorescence decay time constant, yo
and A for this DNA/Trp “sandwich” solutions are 2.3 ± 0 min, 0.4831 and 0.4621, respectively.
Error bars representing the standard deviations are shown.
Figure S9: Same as Figure S8 but for a solution of 1 mM Trp. The fluorescence decay time
constant, yo and A for this DNA/Trp “sandwich” solutions are 12 ± 0 min, 0.7943 and 0.2486,
respectively. Error bars representing the standard deviations are shown.
Figure S10: Same as Figure S8 but for a solution of 1.5 mM Trp. The fluorescence decay time
constant, yo and A for this DNA/Trp “sandwich” solutions are 29 ± 2.1 min, 0.5601 and 0.4676,
respectively. Error bars representing the standard deviations are shown.
S3
Fluorescence Intensity (cps)
1600000
1200000
800000
MB-DNA-Trp
400000
MB-DNA
0
20
30
40
50
o
Temp. ( C)
Fig. S1
S4
60
70
Normalized fluorescence
1.0
0.8
0.6
0.4
0.2
0.0
0
50
100
Time (min)
Fig. S2
S5
150
200
250
Normalized fluorescence
1.0
0.8
0.6
0.4
0.2
0.0
0
50
100
Time (min)
Fig. S3
S6
150
200
250
Normalized fluorescence
1.0
0.8
0.6
0.4
0.2
0.0
0
50
100
Time (min)
Fig. S4
S7
150
200
250
Normalized fluorescence
1.0
0.9
0.8
0.7
0.6
0
50
100
Time (min)
Fig. S5
S8
150
200
250
1.2
Normalized fluorescence
1.0
0.8
0.6
0.4
0.2
0.0
0
50
100
Time (min)
Fig. S6
S9
150
200
250
Normalized fluorescence
1.0
0.8
0.6
0.4
0.2
0.0
0
50
100
150
Time (min)
Fig. S7
S10
200
250
Normalized fluorescence
0.9
0.6
0.3
0
50
100
150
Time (min)
Fig. S8
S11
200
250
1.2
Normalized fluorescence
1.1
1.0
0.9
0.8
0.7
0
50
100
150
Time (min)
Fig. S9
S12
200
250
1.0
Normalized fluorescence
0.9
0.8
0.7
0.6
0.5
0.4
0
50
100
Time (min)
Fig. S10
S13
150
200
250