Chemical Analysis Group Lifecycle
User Information Plan
User Information Plan
Document Information:
Filename
Current Owner
Product Identifier
Project Identifier
USERINFO.DOC
Deborah Perkins
H4033A
ChemStation
Revision Log:
Date
March 24, 2006
April 22, 2007
Reason For Update
Initial Revision
B.03.01 Release
This product is a four day lecture and laboratory course requiring instruments
equipped with diode arrays or VWD’s. This course requires both a student lecture and
laboratory manual. You can use either 1100 or 1200 modules.
Notes to Instructor:
B.02/B.03 now use a sequence container. Please read technical note to understand
implications. Please familiarize yourself with the SL modules.
General Course Information
H4033A
Course Name
Agilent 1100/1200 Series HPLC 3(D) ChemStation Operation
Course Number
Price
Duration
H4033A
$2417.00
4 days
Description
This course is designed for those who want to enhance their skills in the techniques and software operation of
the Agilent 1100 or 1200 Series HPLC 3(D) ChemStation. The course utilizes a lecture format with instrument
laboratories. Topics include creating qualitative and quantitative methods, sequencing, diode array
optimization, and hardware maintenance.
Outline:
Introduction to the HPLC ChemStation and Windows
Laboratory
Creating and Running an Acquisition Method
Laboratory
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Diode Array Optimization
Qualitative Data Analysis
Laboratory
Library Searching
The Diagnosis View and Agilent Diagnotic Tool
Laboratory – Two labs available one for 1100’s and 1200’s one for SL models
Integration
Laboratory
Quantification
Basic Reporting
Laboratory – combined quant and reporting
Creating a Sequence
Laboratory
Sequence Review and Reprocessing
Laboratory
Advanced Reporting
Laboratory
Custom Reporting
Laboratory
OPTIONAL MATERIALS
Commands and Macros
Laboratory
Agilent 1100 Fluorescence Detector
Student Prerequisites
A basic liquid chromatography course such as H1186A, Techniques of HPLC, is required. One month
experience on the instrument is desirable. Profile: An analyst or chemist who has responsibility for operation
and maintenance of the Agilent Technologies 1100/1200 Series HPLC for routine and non-routine samples. A
fundamental understanding of high performance liquid chromatography is expected.
Course File Key
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H4033Ast.doc – student lecture manual
H4033Ast.pdf – student lecture manual for printing
H4033Ainst.doc – student laboratory manual
H4033Ainstlab.pdf – student laboratory manual for printing
H40333Ainst.pdf – instructor manual
H4033Alabinst.pdf – instructor laboratory manual
Datafiles.exe – data files for course – you must load data files into their proper directory.
*.ppt files – Lectures
*.doc – individual lecture modules
See course list next page.
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Course Files
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Course Preparations
Instrumentation Guidelines

2 students per instrument and ChemStation

Instruments can be 1100’s, 1200’s, or 1200 SL’s. Should be equipped with solvent
cabinet, vacuum degasser, pump (binary, binary SL or quaternary), autosampler or
high throughput autosampler, TCC, and DAD.

Appropriate Computer with CD drive and printer. Floppy Disk and drive if possible.

Chemstation loaded with B.03 G2170AA and G2180AA software
License #’s 2170 – TL04041172
2180 - TN040411B9

Provide students with administrator log in and password. Lab indicates password is:
Agilent.

Load course data files and methods into appropriate folders. Do not delete Demo
files.
Other Requirements - Lectures

High resolution LCD Projector capable of handling 1280 x 1024 resolution.

Printed student lecture and lab manual per student.

White board and markers for instructor.

Instructor requires laptop for lectures.
Other Requirements – Laboratories

The following Demo files are required, (no action required on set-up as they load
with software)
 Demo\005-0104.d
 Demo\Demodad.d
 Isocra.m and demo sequence
 Demo\005-0101.D
 Democal1.M
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 Batch.B and Batch.M

Extract Datafiles.exe and load into the following directories:
Chem32\1\Data

Integration.d

Load the folder 03-15-06 (contains a sequence container)

Load the folder 03-20-06 (contains a sequence container)

Lab4m.d
Chem32\1\Methods

Advrep.M
Note: Customers will build several methods and use acquired data files in this course. If
a customer has a problem in one lab and doesn’t successfully create the required .M or .D
file, get a copy from another student’s ChemStation.

Samples
Four vials are required to complete this course.
Use isocratic test mix part number 01080-68704.
For the high standard: dilute 1 mL of test mix to 10 mL
For medium standard: dilute 3 mL of high standard to 10 mL
For low standard: dilute 1 mL of high standard to 10 mL
For Sample: dilute 5 mL of high standard to 10 mL
Distribute the above into autosampler vials for each instrument. Label the vials:
High Standard
Medium Standard
Low Standard
Sample
Recommended Supplies:

Autosampler vials and caps

Pasteur Pipettes

Pipette bulb
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
Classroom Supplies, markers, flip chart, etc.

3 ½ inch floppy, one per ChemStation

HPLC grade water – channel A

HPLC grade acetonitrile – Channel B

HPLC grade IPA

Columns 4.6 X 100 mm C18

Column identification module, part # 7982618-564, one per instrument (optional)

1100 Maintenance and Repair CD-ROM, one/Agilent 1100/1200.

Safety supplies: disposable nitrile gloves, Safety Glasses, Tyvek lab coat, spill kit, eye
wash, universal absorbent wipes, waste labels and Nalgene waste bottles , hood facility
for pouring solvents

Waste Disposal System

Vial Crimper

10 mL graduated cylinder or volumetric flask (suitable for our dilution purposes)
Recommended Spare Parts
Recommended Spare Parts
Agilent 1200

Capillaries

Spare waste tubing

Pump Seals

Pistons

Frits

Solvent inlet filters

Rotors

Needles

Needle Seats

Lamps
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Tools Required















large column wrenches7/16 x ½ and ½ x 9/16
small column wrenches ¼ x 5/16
pozidrive #1
pozidrive #2
ZDV union
Capillary
Small screw driver
Flat wrenches 4mm and 14 mm
Allen wrench set 1.5 mm 2.5 mm 3 mm 4 mm 5 mm
Blanking nut
Small beaker
Safety glasses
Sand paper (small strip)
priming tool
Agilent 1100 Maintenance and Repair CD-ROM
Typographical error corrections, other corrections, and some slide substitutions were made
with the April 2007 revision – these were identified and/or suggested by Ginny Curtis
Additionally, the following edits were made to the course in April 2007:
01_overview:
Added a slide to explain the data organization concept
Added mention of additional paths to the “configure paths” screen
Updated screen capture for Preferences (slide 17). Added instructor notes regarding unique
folder creation on or off.
04_qualanal:
Generated new screen capture and notes for slide 3 – Preferences.
Added a 2nd slide on current vs. DA method
07_integration
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Added a slide to highlight which method is being saved
Updated instructor notes regarding manual integration and da.m
08_quant
Added a slide to highlight which method is being saved
09_report
Added “which method is being saved” verbage to slide 9
10_sequence
Updated screen capture on Preferences slide.
Added a slide on data organization – what it is and what goes into sequence container
Combined customize sequence table slide and sequence table explicit recalibration (replaced
the graphic with add’l columns with graphic of customizing the same list)
Combined Recalibration Settings and Recalibration Report Options slide into one.
10b_sequencereprocessing
Added verbage regarding unique folder creation to slide that outlines reprocessing files
generated < b.01.
Combined slides that point out navigation table and ChemStation Explorer interface
Added a slide covering “which method is loaded during data review”
Added a slide showing “view the acquisition method” in data analysis
Added a slide showing “partial reprocessing” in data analysis
Added a slide showing how to update master or sequence method from navigation table
10b_sequencereprocesslab
Added a warning that the sequence container names are similar (classsequence and
sequenceclass) and to be certain the correct file is being used.
Changed screen capture for Preferences
Template Revision T.04
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Added a few questions about method usage and changing “method to use” from the GUI.
Added instructor notes.
Suggested that G2170-90041 (ChemStation data organization guide) should be added to the
course as an appendix.
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