Resuspension of Plexor™ Primers Instructions
Dry oligos:
1.
2.
Primers containing iso-dC must be stored at a pH greater than 7.0. The Promega Plexor™ Systems
(www.promega.com/plexorresources) include MOPS/EDTA Buffer (1mM MOPS, 0.1mM EDTA, pH
7.4) for resuspension and dilution of primers. The MOPS/EDTA Buffer may also be purchased
separately from Promega Corporation as Cat.# Y5101).
Primers can be diluted to 25X primer stock solutions in MOPS/EDTA Buffer and stored at –20°C as
individual primers or as primer sets. For most Plexor™ assays, the 25X concentrations should be 5µM
(200nM at 1X).
Housekeeping Control Gene Primers:
1.
Primers containing iso-dC must be stored at a pH greater than 7.0. The Promega Plexor™ Systems
(www.promega.com/plexorresources) include MOPS/EDTA Buffer (1mM MOPS, 0.1mM EDTA, pH
7.4) for resuspension and dilution of primers. The MOPS/EDTA Buffer may also be purchased
separately from Promega Corporation as Cat.# Y5101).
2.
Primers can be diluted to 25X primer stock solutions in MOPS/EDTA Buffer and stored at –20°C as
individual primers or as primer sets. For most Plexor™ assays, the 25X concentrations of the
housekeeping primers should be 2.5µM (100nM at 1X).
Disclaimer:
Iso-base containing oligonucleotides are covered by US Patents 6,001,983, 6,037,120, and 6,140,496 and
their foreign counterparts, and licensed to Eurogentec S.A. by EraGen Biosciences, Inc.
EUROGENTEC S.A. • LIEGE Science Park • Rue Bois Saint-Jean, 5 • 4102 SERAING • BELGIUM
Tel.: +32 4 372 74 00 • Fax: +32 4 372 75 00 • E-mail: info@eurogentec.com