A National facility established in 1951
FORM FOR DNA SEQUENCING SAMPLE SUBMISSION
Please contact NCIM by email before sending the samples
Type of sample – Culture/clone library/metagenomic library/ PCR products/plasmid DNA
Type of microorganism- Bacteria/ Fungi/ Yeasts/ Algae/ any other-(Please fill, sign and print and submit hard copy/scanned hard copy)
I] User’s information
Name of Principal
Investigator PI/
Scientist/any other
Prof./Dr./Mr./Mrs.
Address for correspondence
Address for invoice/bill
(if different than above)
Contact information
(STD/ISD code)
Tel, Mobile
Fax
Email
Researcher’s information
(If different than PI)
Number of samples for Partial
sequence (~600 bp) using one
primer
Number of samples for
Complete sequence (more
than 1200 bp) using Forward
and reverse primers
Total Number of samples
(Samples x primer)
Total amount (INR)
Demand Draft details
(Check Fee structure)
Bank details-
DD NumberDateDD (plus Service charge 12.36%) should be in the name of ‘Director, National Chemical Laboratory’, Payable at Pune.
No cash please.
Note: Samples from CSIR institutes/labs will be exempted from service charge.
Samples posted on
dd/mm/yyyy
II] Sample information
(If only strains are provided then fill only template name information. Rest of columns should be blank)
PlasmidPurification method-
PCR product-
Others-
Pleas attach separate sheet for additional samples. Template name and plate name are the same.
Sr.
No.
Strain/Template
provided (e.g. A1)
Template conc.
(ng/ µl)
Only for PCR
product
Product Size (bp)
Only for PCR
product /clone
Sequencing Primer
Provide 10pmol/µl
(Optional)
Do you want to
deposit this strain
in NCIM? If yes
then read #
1
2
3
4
5
6
7
8
9
10
11
# Please attach soft copy of deposit form duly filled. NCIM will accept the strain of industrial and/or taxonomic
value.
Instructions for sample preparation and posting:
Provide template and/or primers in dissolved in PCR grade water.
Do not use EDTA based buffers; it may strongly inhibit sequencing reaction.
Provide samples in 0.2ml/ thin walled tubes.
Use parafilm to tighten the sample tubes or culture plates.
Contaminated culture plates/slants will not be accepted. They need to be pure and possibly well isolated.
III] Template Information: Choose from following.
Purified plasmid DNA (provide 10µl of sample at 75-100 ng/µl)
Purified PCR product (provide 10µl of sample at 30-40ng/µl)
ss -Single stranded M13/phagemid DNA/ (provide 10µl of purified sample at 30-50 ng/µl)
Purified genomic DNA- 200-300 ng/µl in 10 µl volume.
Please limit labels on tube to a max.5 characters.
Enclose a gel picture for quantification and product quality. (OPTIONAL)
Any other primers- please provide 10 µl primers (10 picomple/µl concentration)
Undertaking/Agreement
I/We understood the norms and hence undertake to abide by the sample preparation guidelines. I/We submit the
sample(s) in good faith and NCIM will not be held responsible for loss/damage due to reason(s) beyond its control.
I/We shall give due acknowledgement to the facility in the results so published in thejournals.
Signature of Researcher with date
Signature of Principal investigator
(Date and seal)
FOR NCIM OFFICE USE ONLY
Received by-
Received on-
Material intactness- Good / Bad
Processing person-
Started on-
Sequences ready on-
Results sent to end user on-
Ref/File information-
Note: If PCR product is provided, the failed samples would be re-run only one time free of cost.
Fresh submission of same PCR product will be considered new submission and hence will be charged.
Use chromas lite and similar softwares to open .seq files. Can be freely downloaded from internet. Kindly note
that results are usually provided to the user in minimum 3-4 working weeks or earlier depending upon number
of samples given to us. Users can contact us by email if they do not hear from us within this time.
Imp. Note-Cultures or sequencing samples should reach at NCIM-NCL by speed post or tracking based
overnight shipment; no acknowledgement will be given to end-user. For more information;visit NCIM webpage
or email ncim@ncl.res.inor call at 020-25902670/2454. Address: NCIM resource centre, CSIR-National
Chemical Laboratory, Dr. Homi Bhabha Road, Pune 411008, Maharashtra, India.
Copyright@NCIM-NCL
NC_SeqSub_6_2014