Non-confidential. Please email a copy of your completed application to targets@tech.mrc.ac.uk
MRCT LIBRARY REQUEST FORM
Our aim at the Centre for Therapeutics Discovery:
To progress innovative therapeutic targets emerging from groundbreaking basic research through
identification of tool compounds to validate their involvement in disease processes.
Project Name:
Target Name:
Type of Target (e.g. kinase, protein:protein interaction etc):
Primary Indication:
Principle Investigator:
Organisation:
Address:
Postcode:
Telephone:
Fax:
Email:
If you are an MRC employee please tick
If you receive MRC grant funding please tick
To help CTD assess the most appropriate library for you to screen please complete the following
details. Consideration will be given to
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
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Target class
Screening capacity in your lab
Structural information/ known ligands
Library Request Form
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Version date 04Sep2012
Non-confidential. Please email a copy of your completed application to targets@tech.mrc.ac.uk
Target rationale and background information
Please give details of the molecule or mechanism being targeted and rationale for screening 300 words
What disease is this molecule/mechanism implicated in and what is the evidence for disease association? 200 words
What is the biological rationale that modulation of this target/ mechanism will achieve the predicted effects? 200
words
Library Request Form
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Version date 04Sep2012
Non-confidential. Please email a copy of your completed application to targets@tech.mrc.ac.uk
Screen Proposal
Target Type (please tick as appropriate)
KINASE
GPCR
ENZYME
RECEPTOR
ION CHANNEL
PROTEIN: PROTEIN INTERACTION
TRANSCRIPTION FACTOR
UNKNOWN/PHENOTYPIC
OTHER (please supply details)
Please give details of any known ligands or any structural information for the target. 100 words
Assay Principle: Please give details of assay design, any kits used.
100 words
Read Out (please tick as appropriate)
Fluorescence
Luminescence
Absorbance
Fluorescence Polarisation
Time resolved fluorescence
Imaging
Label Free (please supply details)
CTD can supply libraries in 96 and 384 formats. 20uL 1mM stock in 100% DMSO is provided for screening and
counter screen single point hit confirmation. It is important that a robust screening strategy is in place to
identify true hits.
Assay Stats: (If the assay has not yet been developed, these can be supplied later, prior to any library supply).
Average
Standard deviation
High Control
Background
Signal: background
Z’*
* For single point screening Z’>0.5 is expected. Zhang et al 1999 (J. Biomol screen. 4, 67-73)
Z’ calculated as (1-((3*SD-Hi CTRL+3*SD-LoCTRL)/(Ave-Hi CTRL– Ave-Lo CTRL))
Library Request Form
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Version date 04Sep2012
Non-confidential. Please email a copy of your completed application to targets@tech.mrc.ac.uk
What is the screening capacity of your laboratory? (include details of any screens which have been conducted against
this target already) 200 words
Plate format required: 96
384
Other
What Counter screens are planned for identifying false positives?
100 words
For our proprietary compound libraries a further 20ul 10mM stock of confirmed hits is available for
confirmation in dose response assay. Structures will be revealed after receipt of IC50s.
What secondary assays/ orthologous assays do you have planned to validate your hits.
300 words
Please email a copy of your completed application to targets@tech.mrc.ac.uk, fax to 020 7391 2800 or
alternatively, please post to: Phil Coxon, Target Submissions, MRC Technology, Lynton House, 7-12 Tavistock
Square, London WC1H 9LT.
If you have any queries please call: +44(0)20 7391 2826
Library Request Form
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Version date 04Sep2012