Mutation
1. Assume the DNA strand below is the gene (complement strand) coding for peptide A.
AT GT GGC G C C AT G G G C TCT A A
*
DNA COMPLEMENT STRAND
A) Draw the complete the DNA molecule using the same short hand as used above.
B) Draw the mRNA that would be formed in transcription by the DNA template strand you drew.
C) Write the amino acid sequence of the peptide that would be formed in translation.
D) Assume the G above the * is mutated to a C. Write the amino acid sequence of the mutant
peptide.
E) What kind of a mutation is this?
F) Assume the G above the * is mutated to an A. Write the amino acid sequence of the mutant peptide.
G) What kind of a mutation is this?
H) Assume the G above the * is left out. Write the amino acid sequence of the mutant peptide.
I) What kind of a mutation is this?
2. The diagram below summarizes the beginning of glycolysis, showing the enzymes involved in each
step and the genes that code for them. Assume that the reaction will not proceed at all if the enzyme
is not functional.
Glucose
Gene #1
Gene #2
Gene #3
A
B
C
Glucose-6-P
Fructose-6-P
Fructose1,6-di-P
A) Of what type of organic molecule are genes #1, 2, and 3 made?
B) Of what type of organic molecules are A, B, and C made?
C) What is the function of A, B, and C?
D) If Gene #1 mutated so that A was not able to work at all how would the amounts of glucose,
glucose-6-P, Fructose-6-P, and Fructose 1,6-di-P change?
E) A certain cell is found to have very high levels of glucose and glucose-6-P, but almost no fructose6-P or fructose-1,6-di-P. Which gene is probably mutated in this cell? Explain.
F) A certain cell is found to have very high levels of glucose, glucose-6-P, and fructose-6-P but
almost no fructose-1,6-di-P. Which gene is probably mutated in this cell? Explain.