Curriculum Vitae
Vernon Dolinsky
Citizenship: Canadian
Department of Molecular and Integrative Physiology
7620 Medical Science II 1301 E. Catherine
Lab: (734)647-7721
University of Michigan
Fax: (734)936-8813
Ann Arbor, Michigan, U.S.A. 48109-0622
E-mail: dolinsky@umich.edu
EDUCATION
Post-doctoral fellow, Molecular and Integrative Physiology, since 2003
University of Michigan
Ann Arbor, Michigan, U.S.A.
Project: Role of Liver X Receptors in adipose tissue biology.
Supervisor: Dr. Ormond A. MacDougald, PhD.
PhD., Biochemistry
2003
University of Alberta
Edmonton, Alberta, Canada
Thesis: The Characterization, Regulation and Targeted Deletion of the Murine
Triacylglycerol Hydrolase Gene.
Supervisor: Dr. Dennis E. Vance, PhD.
M. Sc., Biochemistry
1998
University of Manitoba
Winnipeg, Manitoba, Canada
Thesis: Regulation of Phospholipid Remodeling by Thyroid Hormones in the Rat
Heart.
Supervisor: Dr. Grant M. Hatch, PhD.
B. Sc. (Honours), Biochemistry
1996
University of Manitoba
Winnipeg, Manitoba, Canada
PUBLICATIONS
1. DOLINSKY, V.W., Douglas, D.N., Lehner, R. and Vance, D.E. Regulation
of the enzymes of hepatic microsomal triacylglycerol lipolysis and reesterification by the glucocorticoid dexamethasone. Biochem J. 378: 967974 (2004).
2. DOLINSKY, V.W., Gilham, D., Alam, M., Vance, D.E. and Lehner, R.
Triacylglycerol hydrolase: role in intracellular lipid metabolism. Cell Mol.
Life Sci. 61: 1633-1651 (2004).
3. DOLINSKY, V.W., Gilham, D., Hatch, G.M., Agellon, L.B., Lehner, R. and
Vance, D.E. Regulation of triacylglycerol hydrolase expression by dietary
fatty acids and peroxisomal proliferators-activated receptors.
Biophys. Acta 1635: 20-28 (2003).
Biochim.
4. DOLINSKY, V.W., Sipione, S., Lehner, R. and Vance, D.E. The Cloning
and Expression of a Murine Triacylglycerol Hydrolase cDNA and the
Structure of its Corresponding Gene. Biochim. Biophys. Acta 1532: 162172 (2001).
5. Douglas, D.N., DOLINSKY, V.W., Lehner, R. and Vance, D.E. A role for
Sp1 in the transcriptional regulation of hepatic triacylglycerol hydrolase in
the mouse. J. Biol. Chem. 276: 25621-25630 (2001).
6. Taylor, W.A., Xu, F.Y., Ma, B.J., Mutter, T.C., DOLINSKY, V.W. and
Hatch G.M. Expression of monolysocardiolipin acyltransferase activity is
regulated in concert with the level of cardiolipin and cardiolipin
biosynthesis in the mammalian heart. BMC Biochem 2002 3: 9 (2002).
7. Mutter, T., DOLINSKY, V.W., Ma, B.J., Taylor, W.A. and Hatch, G.M.
Thyroxine regulation of monolysocardiolipin acyltransferase activity in rat
heart. Biochem J. 346: 403-6 (2000).
8. Ma, B.J., Taylor, W.A., DOLINSKY, V.W. and Hatch, G.M. Acylation of
Monolysocardiolipin in Rat Heart. J. Lipid Res. 40: 1837-45 (1999).
9. DOLINSKY, V.W. and Hatch, G.M. Thyroxine stimulates the acylation of
lysophosphatidylethanolamine in rat heart. Biochim. Biophys. Acta 1391:
241-246 (1998).
10. Hatch, G.M., DOLINSKY, V. and Ross, T.K. Polyglycerophospholipid
biosynthesis in the heart and H9c2 cardiac myoblast cells.
Research Developments in Lipid Research. 1: 97-106 (1997).
Recent
11. Cheng, P., DOLINSKY, V. and Hatch, G.M.
The acylation of
lysophosphatidylglycerol in rat heart: Evidence for both in vivo and in vitro
activity. Biochim. Biophys. Acta 1302: 61-68 (1996).
ABSTRACTS
1. DOLINSKY, V.W., Lehner, R. and Vance, D.E. The regulation of hepatic
microsomal triacylglycerol lipolysis and re-esterification. Presented at the
Canadian Lipoprotein Conference (2001).
2. DOLINSKY, V.W., Lehner, R. and Vance, D.E. Regulation of the mouse
triacylglycerol hydrolase gene by glucocorticoids. Presented at the
Atherosclerosis Gordon Research Conference (2001).
3. DOLINSKY, V.W., Lehner, R. and Vance, D.E. Characterization of the mouse
triacylglycerol hydrolase gene and the regulation of its expression by
glucocorticoids. Presented at the Canadian Lipoprotein Conference (2000).
4. DOLINSKY, V.W., Sipione, S., Lehner, R. and Vance, D.E. Cloning and
characterization of the mouse triacylglycerol hydrolase gene. Presented at the
American Society for Biochemistry and Molecular Biology Meeting (1999).
5. Ma, B.J., Mutter, T., DOLINSKY, V.W., Taylor, W.A. and Hatch, G.M.
Regulation of Cardiolipin Molecular Remodelling: The Role of Hormones.
Presented at the American Society for Biochemistry and Molecular Biology
Meeting (1999).
6. DOLINSKY, V.W., Sipione, S., Lehner, R. and Vance, D.E. The partial
characterization of the mouse triacyglycerol hydrolase gene. Presented at the
Canadian Federation of Biological Societies Meeting (1998). (P/N)
7. Taylor, W.A., DOLINSKY, V. and Hatch, G.M. Physiological Regulation of
Cardiolipin Remodeling in rat heart. Presented at the Canadian Federation of
Biological Societies Meeting (1998).
8. DOLINSKY, V. and Hatch, G.M. Thyroid hormones regulate the resynthesis
of phosphatidylethanolamine in rat heart. Presented at the Canadian Federation
of Biological Societies Meeting (1997).
9. DOLINSKY, V., Cao, S.-G., and Hatch, G.M. Thyroxine stimulates in vivo
phospholipid resynthesis in rat heart. Presented at the Canadian Federation of
Biological Societies Meeting (1996).
RESEARCH SKILLS
Post-doctoral Project

Cell culture (primary culture and cell lines), including 3T3-L1, ST2 and
C3H10T1/2 cells and the preparation and culture of mouse embryonic
fibroblasts and mouse bone marrow cells as well as the differentiation of
cells into adipocyte and osteoblast cell types.

Analysis of cellular lipid content and cellular mineral production by
colorimetric assays.

Mouse tissue analysis by complete dissection and DEXA scan.

Maintenance of a transgenic mouse breeding colony, involving genotyping and
other methods.

Analysis of gene expression by quantitative PCR.

Experience with various ELISA and LUMINEX assays for the analysis of
cytokines in mouse blood serum.
PhD. Project

Extensive experience in molecular cloning and southern blotting, involving
restriction mapping, cloning and creation of a targeting vector for the
murine triacylglycerol hydrolase gene

Experience in the creation of cDNA libraries and cloning of cDNAs

Analysis of expression through Northern and Western blotting as well as
real time PCR.

Analysis of transcriptional regulation, utilizing nuclear run-on, electromobility shift assays and the creation of promoter-luciferase reporter gene
constructs.

Experience with cell culture (primary cultures and cell lines), including
stable and transient transfections

Have worked with rats and mice, involving hormonal and dietary studies,
tissue isolation and preparation of primary hepatocytes
M. Sc. Project

Experience with basic enzymology, including developing enzyme assays

Lipid analysis, including gas chromatography and thin-layer
chromatography

Isolated and perfused rat hearts for in vivo analysis of lipid synthesis
HONOURS AND AWARDS
Alberta Heritage Medical Research Foundation Studentship 1997-2002
Medical Research Council of Canada Studentship
University of Alberta Walter Johns Tuition Scholarship
1998-2001
1997-2000
Novartis- Canadian Lipoprotein Conference Student Presentation Award
2000
Merck Frosst- Canadian Society of Biochemistry and Molecular
Biology Student Travel Award
1999
Manitoba Health Research Council Studentship
1997
Merck Frosst- Canadian Society of Biochemistry and Molecular
Biology Student Travel Award
University of Manitoba, Faculty of Science Dean’s Honour List
1996
1994-1995
ASSOCIATION MEMBERSHIPS
American Oil Chemists Society
1996- 2002
Canadian Society for Biochemistry, Molecular and Cellular Biology 1996- 2002
TEACHING
Laboratory Demonstrator, Advanced Biochemistry Laboratory
Department of Biochemistry, University of Alberta.
1998-1999