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List of Accepted Abstracts
The First Part
Traditional
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19.
Chinese Medicine
Overview of Traditional Chinese Medicine-----------------------------------Dong Jingcheng （1）
The Basic Structure of Traditional Chinese Medicine-----------------------Dong Jingcheng （4）
The Future of Traditional Chinese Medicine---------------------------------- Dong Jingcheng（5）
The Literature Research of Ming Dynasty Physicians Treating Chest Pain
Syndrome------------------------------------------------------------- Li Fangshuai, An Dongqing（6）
The Preliminary Studies on Herbal Medicine Resources of Aconitum from Yili and Easten
Region in Xinjiang------------------------------------------------------Liu ShuMin, Nie Jihong （7）
Zhisousan
treating
infections
cough
randomized
clinical
trials---systematic
reviews----------------------------------------------Li Fengsen , Jing Jing, Li ChunYan, et al （8）
120 Cases of clinical study on Side-lying position bowed their heads and shaking method in
treating Cervical Spondylotic Radiculopathy----------------------------------------Liu JunChang,
Muhetaer Ayoufu, Wang Xinjun, et al （8）
Body Constitution Classification (BCC) of Coronary Heart Disease (CHD) in the East, South,
North of Xinjiang-------------------------------------------------------------Li Hui, An Dongqing（8）
Case-control Study of The Association between Northwest Dryness Syndrome and Chronic
Gastritis-------------------------------------------------------------Zhang Huitian, Zhou Mingxin（9）
Effect of dipeptidyl peptidase IV in 3 extracts from plants of Xinjiang----------------------Yang
ZhenZhen, Wang XueHua, Wang Ye, et al（10）
Effects of TianXiangDan on blood lipid and vascular endothelial growth factor in the
arteriosclerosis in ApoE (-/-) gene knockout mice----------------- Jiao Yujie, An Dongqing（11）
Primary study on chemical composition and biological activity of Arctium tomentosum
Mill----------------------------------------------------------------------Zhang Haoke, Tian Shuge（11）
Quantitate Chemical Composition and Chemical Pattern Recognition in Coriopsis Ticntoria
from Different Areas----------------------------- Sun Zizeng, Zhang YanLi, Aytulun Simayi（12）
Regulation Effect of OboBiyanChongji on Expression of IL-2, IFN-γ, IL-13 and Nasal
Mucosa in the Experimental Allergic Rhinitis in Rats-------------Jiang Liming, An Yang （12）
Research on Changes of Different Contents in Fried Aconitum pendulum Busch---------------Wu Lin, Zhang Fan（13）
Research on Xinjiang Region Physical Type of Patients with Hyperuricemia on Behalf of Tea
and Chinese Medicine Intervention--------------------------------------- Li Li, An Dongqing（13）
Safety Evaluation of Awei Capsule: Assessment of Acute and Subchronic Toxicity in
rodent-------------------------------------------------Han Rong, Xue Jie, Nurmuhammat Amat（14）
Sophora alopecuroides extract on human coloncancer cell line HCT116 strains of chick
embryo chorioallantoic membrane experimental studyon tumor angiogenesis--------Li Jingjie,
Lv Shuqin（15）
Study on the difference between Uighur ethnic group and Han people who suffered from mild
cognitive impairment (MCI) with electroacupuncture therapy-------Guo Hui, Liu Zhiyan（16）
1
20. Study on the Pharmacognosy, Chemistry and Acute Toxicity of Aconitum soongaricum
Stapf---------------------------------------------------------------------Cai DongMei, Zhang Fan（16）
21. Study The Best Extraction With AngeLica From Knee and ArthraLgia HerbaL Paste
--------------------------------------------------------------------------S.Yun, G.Liang, W.XinJun（17）
22. TCM hung drag line drainage is the treatment of high anal fistula-----------Chang Zhongsheng,
Yu Dandan （17）
23. The Effect of Sophora Alopecuroides Extract to the Receptor Tyrosine Kinases in Colorectal
Cancer Cell Lines-----------------------------------------------------------Jiang Qin，Lv Shuqin（18）
24. The Clinical Observation of Tianxiangdan Capsule in Treating Angina Pectoris in Coronary
Heart Disease (Syndrome of Qi Deficiency and Blood Stasis)-------------Song Xiaopeng，An
Dongqing（18）
25. The experience of using dihuang pills to treat the chronic cough and asthma treatment of
lung-------------------------------------------------------------------------------------Shi Suofang（19）
26. The influence of p38 MAPK pathway to asthmatic guinea pigs which Qufeng Xuanbi
made-------------------------------------------------------------------------------------Shi Suofang（19）
27. The Studies of Regulating Blood Lipid and Antioxidant of Awei Capsules on Hyperlipemia
Rats-----------------------------------------------------------------------------Cui Liang, Xue Jie （20）
28. The study of quality standards and determination of validity period of Awei
capsules----------------------------------------------------------------------Hou MingQi, Xue Jie (21)
29. The Study on Medicinal Value and Reduce the Toxicity with Processed of Aconitum
soongaricum Stapf in Xinjiang--------------------------------------------- Wu Chao, Jiang Lin（21）
30. The study on sustained-release technology of Tianxiangdan capsules----Wang Jia, Jiang Lin（22）
31. Theory of Acupuncture "reinforcing" and "reducing"----------------Cai Hua, Yin GaiZhen（23）
32. Assessment of using the Tianxiangdan Jiaonang Helpling Rehabilitation Therapy in Coronary
Artery Bypass Grafting---------------------------------------------Li Jingru, Wang Xiaofeng （24）
33. Clinical study of Differential Treatment of Traditional Chinese Medicine in Treatment of 210
Cases of Sub-health State in Urumqi city-------------------------------Liu Tao, Hu Xiaoling（24）
34. Coronary Heart Disease merge 2-hour postprandial glucose patient of Traditional Syndrome
Differentiation and ZeXieDanMingYin intervention research---------------Zeng Lin, An
Dongqing, Li Kaili, et al（25）
35. Experimental Study in Effects of Xu-zao-geng-ping Powder on the Histomorphology Change
of Arteries and MMP-9, TIMP-1 in Climacteric Hypertension Rats---Li Hongxia, Lin Xue（26）
36. Experimental study on inflammatory factors in serum of rats with Rheumatoid Arthritis
treated by PengCao Removing Bi-syndrome Decoction------- Tian Xinwei, Liu Chunjing（27）
37. Study on Influence of Xu Zao Geng Ping granule on Blood Pressure Variability of the Rats
with Menopause Hypertension-------------------------------------------Gan Qinglei, Lin Xue（27）
38. The characteristic of the syndrome TCM distribution of femoral head necrosis in Urumqi
region-----------------------------------------------------------------Jin Wuyong, Meng Qingcai（28）
39. The Correlation of Uighur and Han Nationality of TCM Syndrome Type of Premature
Coronary Heart Disease with Blood Lipids and Inflammatory Factor----------------Zhai
Xueqin, Wang Xiaofeng（29）
40. The Correlation of Uighur and Han Nationality of TCM Syndrome Type of Premature
Coronary Heart Disease with Molecular Markers of Prethrombotic State----------------Liu
Hao, Wang Xiaofeng（30）
2
41. The experimental study of Qihong Decoction on the effects in rat model with isoproterenol
induced chronic heart failure-------------------------------------Wang Min, Wang Xiaofeng （31）
42. The Influence of Oubo Rhinitis Granule to Allergic Rhinitis Rats Th2 Cells and Its Factors、
IgE and Mast Cells---------------------------------------------------- Zhang Xingri, Li Yanhua（31）
43. The pathological effect of PengCao Removing Bi-syndrome decoction on synovial
inflamation and bone erosion of rats with Collagen-Induced Arthritis------------Chen Chang,
Liu Chunjing（32）
44. Treatment of QHD Tongluo Experimental Study of hyperuricemia-------Wang Danhui,
Yushanjiang Aikemu（33）
45. The Influence of Xihong Kang Fang Using to the diabetes nephrosis patient phase III Hcy
CysC and UAER----------------------------------- Feng Chengcheng, Yushangjiang Aikemu（33）
46. Clinical Analysis of 200 Cases of Curative Effect Factors on Treating Knee Osteoarthritis
with Small Needle Knife-------------------------------------------------------Ma Lei, Meng
QingCai（34）
47. The Study ofClinical Curative Effect Research of Jing Yi An Shen Powder on 104 Cases of
Insomnia Treatment by Acupoint Application-------------------------------------Wei Qing, Hu
Xiaoling(35)
48. Clinical Curative Effects of Repeated Shallow Puncture with Filiform-fire Needle
on Herpes-zoster-----------------------------------------------AyijiamaliTuohuti, Liu Hongxia（35）
49. Clinical Effect of DLK-A for Treatment and Prevention of Metabolic Syndrome(MS), on
Blood Sugar, Blood Pressure, Blood Lipids and Weight------------------Palidan Wushuer,
Nurmuhammat Amat, Duolikun, et al(36)
50. Clinical observations on chronic prostatitis of dampenss-heat down-flowing stasis with
qianlieshuang decoction--------------------------------------------------------Sha Mo, Ma Chao(37)
51. Clinical Study on Traditional Chinese Medicine combined with Acupuncture of Promoting
the Recovery of Gastrointestinal Function in the Treatment of Postoperative Patients
with Damp-heat Type ---------------------------------------------------Guli Shaera, Liu Zhiyan(38)
52. Decoction of Bushentongluo and Decoction of its Decomposed in Repair of Articular
Cartilage Defects in Rabbits-------------------------------------------------Li Leijiang, Lu
Yong(38)
53. Effect of Jangzhuoshengqingtang on Pathology organization morphological、plaques area
and Inflammatory Reaction in Atherosclerotic in Aorta of Apo-E-gene knockout
Mice---------------------------------------------------------------------Wang Jipeng, Xin Xiaoyi(39)
54. Experimental Study on Effect of Aplastic Anemia Model Rat’s Regulation Factors IL–2、
TNF–a Treated with Tonifying Kidney and Replenishing Essence Method---------------Xie
Rong, Shan Lijuan (40)
55. Literature Research on Prescription Rule of Oral Drugs for Osteoarthritis-----------------Chu
LiTao, Lu Yong(41)
56. Primary Immune Thrombocytopenia Different Syndrome Types and Platelet Parameters of
Characteristics Research--------------------------------------------Wang Yahong, Bai Yusheng(42)
57. Qufeng antispasmodic to asthma scattered (wind phlegm wheezing) patients with pulmonary
function, FeNO influence----------------------------------------------Bai WenMei, Li Fengsen(52)
58. Research on Catharsis and Moisturizes Theoretical Literature of Chinese Medicine
Skin---------------------------------------------------------------------------Chen Lu, Zhou Guang(43)
3
59. Spleen detoxification soup murine spleen deficiency type psoriasis ET-1, E-selection and of
PCNA influence-----------------------------------------------------Guo Shanshan, Liu Hongxia(44)
60. The Comparison on the Location of Insomnia According to Different Classification
Traditional Chinese Medicine of Five-god Theory and Four Examinations---------Fan
Xiufang, Zhang Xingping(45)
61. The comparison research of acupuncture and acupoint application in patients with AECOPD
and their influence of lung function and quality of life -----------Shao Meimei, Liu Zhiyan(45)
62. The effect of jianpijiedu decoction on the vascular active factor of psoriasis model
mice------------------------------------------------------------------------ Gao Yuan, Liu Hongxia(46)
63. The Experiment of Jian Pi jiedu decoction on Guinea Pig Model of Psoriasis with Spleen
Deficiency Syndrome-----------------------------------------------------Guo Fei, Liu Hongxia(47)
64. The Experimental Study of Huangqi Guizhi Wuwu DecoctionT herapy on Failed Back
Surgery Syndrome-----------------------------------------------------Liu Kaiying, Huang Yifei(48)
65. The Impact of Yangyin Huowei Heji on Chronic Atrophic Gastritis Patients' Gastroscope
Histopathology and Hp-------------------------------------------Ding GuoNing, Zeng Binfang(48)
66. The Research of the Inhibition of Invasion and Metastasis of Colon Cancer Cells HCT116
after Administrate YiQiFuShengFang by ERK/MAPK Signaling Pathway-------------Han
Jing, Lv ShuQin(49)
67. The Study on Inflammatory Factors Therapeutic Effect of DangGuiNianTongTang Pasty with
MTX on Adjuvant Arthritis in Rats-----------------------------------------Yuan Li, An Liping(50)
68. The Clinical Research of Acupuncture Combined with Auricular Acupuncture for Patients
with Acute Exacerbation of COPD to Improve their Quality of Life and Pulmonary
Function------------------------------------------------------------------------Cao Lin, Liu Zhiyan(50)
69. Traditional Chinese Medicine theory and gynecological dietotherapy put medicine law
research-----------------------------------------------------------------------Tan Rong, Liu Ailing(51)
70. Distribution characteristics of TCM syndrome and treatment on 2026 Cases of Sub-health
Population in Urumqi City-----------------------------------------------Du Shuai, Hu Xiaoling(53)
71. Yang-yin-huo-wei Formula Treatment of Chronic Atrophic Gastritis Patients Report
Outcomes and Quality of Life in Research -------------------------------------------- Dai Ming,
Zeng Binfang(53)
72. Anti-hypertensive effects of the aqueous extracts of Chinese yam (Dioscorea opposita Thunb.
） in Two-Kidney, One-Clip Renal Hypertension Rat model-------------------------Nurmuhammat Amat, Palidan Wushuer, Sajida Abdureyim, et al(54)
73. Effect of TongFeiJingTanFang on Immunologic Function of Phlegm Heart Syndrom in
Chronic Obstructive Pulmonary Disease-------------------------Bai Zhengping, Tan Guangbo,
Hu Xuejun(55)
74. Effects of “ Yang-Warming and Kidney Essence-Replenishing Herbal Paste ” on
Cold-related Asthma Exacerbation——A Report of 74 Cases-------- Tang Binqing, Shi
Kehua, Li Xiaoqi, et al(56)
75. Huang Di Nei Jing health theory and the prevention and treatment for chronic obstructive
pulmonary disease-----Xu YanLing56()
76. Influence
of
Compound
Panax
notoginsenosideGranules
(CPNG)
on
bleomycin-inducedpulmonary fibrosis in rats-------------------Ling Chunhua, Wu Juanjuan(57)
77. Qufeng Xuanfei side clinical study of treatment of allergic cough ----------------------Xu
4
78.
79.
80.
81.
82.
83.
84.
85.
86.
87.
88.
89.
90.
Lihua, Li Lei, Huang Ye, et al(57)
Randomized Control Trial of JinhuaQinggan Granule in Treating Influenza with Wind-Heat
Affecting Lung Symptom-Complex------------------------Li Guoqin, Liu Zhiguo, Liu
Qingquan(58)
Scorpion - centipede on the rat model of asthma airway inflammation and airway remodeling
in experimental research ------------------------Li Haiyan, Yang Peilan, Huang HaiYin, et al(58)
The application of Lung and Large intestine Interacts relations in COPD------------Geng
Limei(59)
The effect of Er Chen Jie Han Particle for the treatment of OSAHS patients with Phlegm
Damp Syndrome-----------------------------------------------------Wang Zhen, Yang Junchao,
Shen Yingying, et al(59)
The search of basic law of the ancient physicians using the prescription medication to treat
"Pulmonary heart disease in the remission stage "----------------Hu Xuejun, Bai Zhengping,
Tan Guangbo et al(60)
Rharmacodynamic Research on Liver Protecting, Choleretic and Immune Regulating
Function of Deduhonghua-7 Powder------------------BA Tu-de-li-gen，WU En-bao-yin，NA
Ri-su, et al(61)
The Clinical Study Between Syndrome Type in TCM and the Serum Levels of IL-25 and
Total IgE in Patients with Bronchial Asthma--------------------------------Liu ZongWei, Zhou
XianMei(61)
Clinical Observation on Chronic Obstructive Pulmonary Disease of Acute Aggravating Stage
Treated with Compound Fuercao mixture-------------------Shi KeHua, Zhe Zhe, Lv MiMi, et
al(62)
Effective cases analysis of Professor Wu Yin-gen in differentiating and treating interstitial
lung disease-----------------------------------------------------------------Shi KeHua, Xiong Bidan,
Yu Xiao, et al(63)
Experimental Study of Type 2 Diabetes Animal Models through Decoction of
Baihuginseng-chickpea Mixture------------------------------------------Ji Yan, Wang Cunfen(63)
Preliminary study on Preparation Technology、Quality Standard and Pharmacodynamic for
Nigella Glandulifera Freyn Particle------------------------Xiao Qiong, Jiang Lin, Li Xiaojin(64)
Preliminary Study on the Chemical Composition of Nigella Glandulifera Preyn-------- Xu
Jingjing, Xue Jie, Jia Xiaoguang, et al(65)
Kidney’s Concept of TCM and Mechanism, Method, Prescription, Drug & Application of
Kidney-invigorating------------------------Wang GenFa, Dong JingCheng, Duan XiaoHong(66)
The Second Part
Uygur Medicine
91. Is “abnormal black bile” merely imaginary philosophical concept that has no medical
meaning?-------------------------------------Halmurat Upur, HR Tang, Batur Mamtimin, et al(66)
92. Bioactive fraction of Abnormal Savda Munziq and its antiproliferative activity on HL-60
cells----------------------------------------- Murat Kizaibek, M.Baranov, Halmurat Upur, et al(68)
93. Effect of Xiatara on ultrastructure, protein and ergosterol biosynthesis in
5
dermatophytes----------------------------------------------------Deng ShuWen, Halmurat Upur(69)
94. Establish the Alzheimer's disease carrying abnormal savda syndrome rat model and observe
the effects of formulae on it-----------------------Palidan Wushuer, Nurmuhammat Amat,
M.Baranov, et al(71)
95. Establishment of hepatocarcinoma carrying abnormal savda animal model and it`s
morphological----------------------------------------------------Yusup Tuerxun, Halmurat Upur(72)
96. Experimental Study on Caries Prevention by Aleppo Gall----------Z.Jin, Halmurat Upur,
Baranov M, et al(73)
97. General toxicology studies of Xi pa yi Kui Jie’an enema------------------------ Kasimjan
Aximjan, Halmurat Upur(73)
98. Abnormal Savda Munziq and Munshil’s effect on the gene regulation, oxidative stress
induced cell damage and apoptosis-------------------- L.Lin, L.Gogol, Halmurat Upur, et al(74)
99. Mechanism research of Gujingmaisiha treatment for premature ejaculation (PE)----Song
GuoHong, S.Nelski, Halmurat Upur(75)
100. Metabonomic studies of abnormal Savda syndrome complexity diseases in Traditional
Uighur Medicine------------------------------------------- Xu WeiFang, N.Tatiana, T.Aji, et al(76)
101. Studies on platelet parameters and hepatic function changes of COPD patients with syndrome
of kidney deficiency and stagnation of phlegm---------------------- You Na, Halmurat Upu(77)r
102. Studies on the Anticancer and Reversing Multidrug resistance effects of Abnormal Savda
Munziq Total Flavonoids--------------------------- Maynur Eli, M.Baranov, Halmurat Upur(78)
103. Study on anti-tumor mechanism of Abnormal Savda Munziq in vivo based on transplanted
S-180 and EAC tumor model----------------------------------Anwar Aikemu, Halmurat Upur(79)
104. Study on the Possible Mechanisms of Xinjiang Red Raspberry Fruit Extract in Reducing
Blood Pressure and Alleviating Oxidative Stress in SHR Rats--------------------------- H.Jia(83)
105. The Abnormal Savda Syndrome CD41, CD62p and fibrinogen clinical analysis of
change---------------------------------------------------------------Cao XiaoBin, Halmurat Upur(85)
106. The clinical report of Awrixim syrup to treat the Vasculitis patients, one case-----G.Abduriyim, P.Ablimit, R.Jappar, et al(85)
107. The Study of Basic Exploration and Application of Uighur Medicine Xipayi Mouth Rinse in
Treatment of Gingivitis --------------------------------Habiba, S.Nelski, Halmurat Upur et al(86)
108. Study on the anti-inflammatory and anti-asthmatic mechanism of Hyssopus officinalis.L
active fractions---------------------------------------------- A.Nurahmat, N.Rozi, A.Rozi, et al(87)
109. A study of the effects of the leaf extract of Cydonia Oblonga Miller on blood hemostasis,
coagulation and fibrinolysis in mice.------------------------Anwar Umar, Zhou WenTing, Ibadet
Tohti, et al(88)
110. About the exploration of Bloodletting therapy of Uighur Medicine------------Amier Alye,
Tuersunjian Tohsun(89)
111. Analysis of intestinal bacterial communities in four group abnormal hilit hyperpietics and
normal hilit of Uighur nationality---------------------------Jiang Wei, Halmurat Upur, Delixiati
Yimiti, et al(89)
112. Ancient Uighur drug treatment of coronary heart disease medication compatibility
law----------------------------------- Mamatyili Rumat, Aniwar Talip, Abduwar Abulak, et al(90)
113. Anti-carcinogenic effects of the polyphenol of Abnormal Savda Munziq in association with
its cytotoxicity, apoptosis-inducing properties and telomerase activity in human cervical
6
cancer cells (SiHa)------------------------------- G.Abliz, Ahmatjan.Mamat, W.XinLing, et al(90)
114. Cognitive enhancement and neuroprotective effects of Abnormal Savda Munziq, a
formulation of Traditional Uighur Medicine, in a rat model of Alzheimer ’ s disease
-----------------------------------------Palidan Wushuer, Nurmuhammat Amat, Halmurat Upur(91)
115. Composition of the essential oil and antioxidant activity of several extracts of Moluccella
laevis L. (Lamiaceae) --------------------------------Güvenç, A, Özek, G, Hürkul, M.M, et al(92)
116. Composition of the essential oil and antioxidant activity of several extracts of Moluccella
spinosa L. (Lamiaceae) -------------------------------Güvenç, A, Özek, G, Hürkul, M.M, et al(92)
117. Determination peptides compositions of Chickpea Sprouts by high performances capillary
electrophoresis------------------------------------------A.·Yili , Q. L. Ma ， Zhang Junping, et
al(93)
118. Effect of Cydonia Oblonga on experimental Thrombosis in rats------------Zhou WenTing,
Anwar Umar, Wuliya Yimin, et al(94)
119. Effects of Ocimum Basilicum L. extracts on Lipid Metabolism and Oxidant Status in
Hyperlipidemic rats---------------------------------------------Anwar Umar, Zhou WenTing,
Wuliya Yimin, et al(94)
120. Effects of soybean peptide on neuroendocrine immune network, memory, hematology and
plasma biochemistry in young healthy volunteers----------------Nurmuhammat Amat, Palidan
Wushuer, Guzalnur Imam, et al(95)
121. Experimental study on Vasodilation of Traditional Uighur Herb for Vitro Vascular--------Lan
Wei, Jing YuXia, Halmurat Upur(96)
122. Retrospective analysis of hypertension and hypertensive intracerebral hemorrhage in hospital
-ized patients of Uighur and Han nationnality in Hami district of Xinjiang----------------Aniwar,
Abulymit, Wang Kun, et al(96)
123. Hepatoprotective effects of Cichorium Glandulosum leaf extract against
d-galactosamine-induced Liver injury in mice----------------Imam G, Yusupjan, Aynur, et a(97)l
124. Influence of the Uighur Medicine sand therapy on hemorheology of the rabbit experimental
knee osteoarthritis and FEM analysis---------Mahemuti D, Halmurat Upur, Kong CY, et al(97)
125. Inhibitory effect of Pomegranate Blossom Flower (PGf) extract on Influenza virus
neuraminidase activity and induced Cytotoxicity--------------------------------------Lan Wei，
Lan WenJun，Halmurat Upur(98)
126. Preparation and in vitro anti-cancer activities of flavonoids from Xinjiang Glycyrrhiza Inflata
------------------------------------------------ Mourboul Ablise, Sheng Lei, Rena Kasim, et al(98)
127. Report of the eels and other drug treatment of facial paralysis embolism---------------- Mamat
Hujy, Gao Jianping(99)
128. Screening of quantitative diagnosis indices of Typing Abnormal Hilit syndrome in Uighur
Medicine using delphi method to survey: 180 cases of malignant tumor------------Ayinuer
Mamatisidike, Halmurat Upur，Erxiding, et al(100)
129. The curative effects of 51 cases with Uighur Medicine therapy on Urticaria------- Mahmutjian
Ahmat, Mamatjian Zayit(100)
130. The systematic reviews of the Uighur Medical abnormal humoral typing to the treating knee
osteoarthritis--------------------------------------------- J.XiaoYue, M.kelimu, G.ShiMin, et al(101)
131. Treatment of 225 cases of hair loss according to Uighur Medical temperament theory analysis
alopocia-----------------------------------------------------------Sidik Ramat, Nuermanguli Aly(101)
7
132. Tradition, current situation and perspective of traditional Uighur Medicine in
China---------------------------------Nurmuhammat Amat, Palidan Wushuer, Aziz R A, et al(102)
133. Treating report about 190 patients with kidney stone patients treated by Uighur
Medicine---------------------------------------------------------------------Abduwayit Nurahmet(102)
134. A clinical report of 31 cases using Uighur Medical treatment of herpes zoster ------------Nuermangul Alya , Sidik Ramat, Tajigul Mama(103)t
135. Uighur Medicine fusion of East-West cultural factors-------------------------Wang XingYi(103)
136. The effect of Abnormal Savda Munziq on Hippocampus BDNF, 5-HT1A mRNA expression
in
depression
animal
models
and
Abnormal
Savda
Syndrome
animal
models-------------------Upur Hanzohra, Abduwayit Nurahmet, T.Kamil, et al(103)
137. The construction of the Uighur pharmaceutical scientific research information support
platform system based on Cloud Technology------------------- ZhouYi, Dilixiati Yimiti, Luo
XueQiong, et al(104)
138. Chemical Constituents in Essential Oil from Hyssopus officinalis.L by Steam Distillation and
SFE-CO2 by GC-MS------------------------Mahsum Ekim，Nurjan, Abdiryim Yusup, et al(104)
139. Preliminary study on the anti-inflammatory effect of the extracts of Hyssopus
officinalis.L----------------------------------Mahsum Ekim，Nurjan, Abdiryim Yusup, et al(105)
140. Abnormal Savda Munziq Improves the Biological Syndromes of CUMs induced Depression
Animal Models------------------------Abdiryim Yusup, Nurahmat A, Guzalnur Imam, et al(105)
141. Abnormal Savda Munziq, an Herbal Preparation of Traditional Uighur Medicine, Regulates
the
Neuroendocrine
Function
of
CUMs
induced
Depression
animal
models------------Abdiryim Yusup, Hanzohra Upur, Guzalnur Imam, et al(106)
142. Clinical Research on Treatment of Knee Osteoarthritis by the instrument of Heat Conduction
Sand instrument in Uighur Medicine----------------------------He ChunHui, Halmurat Upur(106)
143. Effect of pomegranate ethyl acetate extract on the function of rat islet cells --------- Yao Hai,
Yang WeiXing, Xu YongHua, et al(107)
144. Establishment of hepatocarcinoma carrying abnormal savda animal model and it`s
morphological study-------------------------------------------Yusup Tuerxun, Halmurat Upur(107)
145. Metabonomic Studies on Abnormal Savda Patients with Type 2 Diabetes Mellitus Using
NMR Spectroscopy------------------------------- Mao XinMin, Lang Jun, Ma XiaoLi, et al(108)
146. Studies of chemical constituents and antioxidant activity of Jujubae Fructus in Xinjiang
---------------------------------------------------------------------Wang DongDong, Tian Shuge(109)
147. Studies on Chemical Constituents and anti-tumor of essential oil from the seeds of Ferula
sinkiangensis -----------------------------------------------Wang Yue1, Jiang Lin1, Li Xiaojin(109)
148. Study on Quality Standard and Fingerprint of Ziziphora clinopodioides Lam-------Yu Qian,
Tian Shuge(110)
149. Study on the Uighur Medicine Compound of the Anticancer Activity Parts of Abnormal
Black Munziq—Based on the “TCM Components” Mode------------Yang Juan, Zhang
Fan, Halmurat Upur(111)
150. The impact of Hypoglycemic Ziyabiti Tablet (HZT) extracts on type 2 diabetes mellitus rats
and its activity in cultured liver cells---------------------- Li Guirong, Fan Yong, Wang
XiaoFeng, et al(112)
151. The study on the development of the Xipayisolid-gingival buccal tablets---------Ma Pingping,
Nie JiHong(113)
8
152. Toxicological evaluation aqueous extract of the galles of Quercus infectoria olivin
rodent-----------------------------------------Mubarak, Nurmuhammat Amat, Kasimjan, et al(113)
153. Dialectical Analysis of exercise-induced fatigue patients according the Uighur Medicine
humoral quality ----------------------------Ailyjan Asla, Meng Qingcai, Liu Zhenfeng, et al(114)
154. Comparative study Uighur Medicine sand therapy with other regional sand therapy， sand
characteristics----------------------Abudusalam Saidula, Osman Yasin, Satar Kurban, et al(115)
155. The Relationship between toxic electron microscopy examination and alkaloid amounts in
Datura stramonium L seeds of Xinjiang------------ R.slam, Y.Mamat, A.Abdugheni, et al(115)
156. Effect of psychological factors on vitiligo patients’hypothalamus-pituitary-adrenal axis and
intervention study----------------------------------------------- Aikebaier Anzhaer 1, Silafu Aibai
1*, Tuerxun Wufuer, et al(115)
157. Arthroscopic Debridement Combined with Uighur Maizhuni Treatment of Knee
Osteoarthritis andomized Controlled Trials--------------------------Yuan Guoxiang, Lu
Yong(116)
158. Association analysis Uighur Medicine's combined modality therapy with ordinary psoriasis
patien's cytokine and immunologic mechanism-------------- Riziwan Wumaier 1, Aikebaier
Anzaer 1, Silafu Aibai, et al(117)
159. Study On Xanthine Oxidase Inhibitory Actvity In Capparis Spinosa.L By
Spectrophotometry---------------------------------- A.Rahman,A.Tax, Nurmuhammat Amat(117)
160. Clinical effecacy observation Uighur Medicine Preparetion Denar on the patients with viral
hepatitis with Cholestatic syndrome------------------------------------Dmytriy Sebrebryakov(118)
161. Clinical Observation of Wujing Ointment in Reducing Adverse Reactions attached to
Asthenia of Spleen and Pathogenic damp of FOLFOX4 in Treating Cancer of Colorectum
------------------------------------------------------------------Xiao ChunXia,Zhang HongLiang(119)
162. Clinical research of auxiliary therapy effect of Turnips on tuberculosis patients------ A.Pattar,
Nurmuhammat Amat，Halmurat Upur , et al(119)
163. Clinical Therapeutic Efficacy and Security Assessment of Uighur Medical treatment for
Vitiligo with Uighur Medicine-------------------------------------- Tursun Wuphour, Yüsup
Metenur, Silafu Aibai, et al(120)
164. Correlation Analysis of Syndrom Differentiation of Vitiligo and Its Munzich and Mushil
Therapy Based on Traditional Uighur Medicine Theory------- Silafu Aibai, Tuerxun Wufuer,
Aizezi Yasen, et al(121)
165. Detection of neuroendocrine and immune factors before and after traditional Uighur
Medicine treatment of vitiligo Uighur and it’s significance---------- Aikebaier Anzhaer,
Silafu Aibai, Tuerxun Wufuer, et al(122)
166. Discussion on the relationship between vitiligo and peripheral blood in traditional Uighur
Medicine------------------------------------------------------- R.Umar, J.Abduwayet, T.Tursun(122)
167. Effect of Euphorbia humifusa active component on the cell membrane biosynthesis and gene
expression of metalloprotease(MEP), subtilisin(SUB) of Trichophyton rubrum------ Li
ZhiJian, Zhao MingYue, An HuiXia, et a(123)
168. Effect of Uighur Medicine Maizhuni on the Expression of MMP-1, MMP-3, MMP-13,
TIMP-1, IL-1β, and Aggrecan in Rat Osteoarthritis Models-------------Zhang Yaowu, Meng
QingCai(123)
169. Research of Quality Control about Abnormal Savda Munziq Granular andα-Glucosidase
9
Inhibitors--------------------------------------- Xin LuDe, Tian ShuGe, Halmurat Upur, et al(124)
170. Retrospective Survey and Analysis on Ulcerative colitis Treated with Uighur
Medicine-------------------------------------------- Turgun Amar, Jurat Tohti, Sirap Abai, et al(125)
171. Retrospective Survey, Research and Analysis on Osteoarthritis of the Knee treated with
Uighur Mdicine--------------------------------------------------------- Jurat Tohti, Turgun Amar,
Sirap Abai, et al(125)
172. Studies on Anti-Aphthous Ulcer and Mechanisms of Galla Turcica Extract treating Recurrent
Aphthous Ulcer in experimental animals------------------ Fan XiaoHong, Li ZhiJian, Silafu
Aibai, et al(126)
173. Study of in vivo - in vitro Anti- Influenza virus and Anti-inflammation and immune function
of Artemisia rupestris extract-------------------------------- Meng FanLong, Li ZhiJian 1,2, Silafu
Aibai, et al(127)
174. The Nymphaea alba with oxidative damage protection to human umbilical vein endothelial
cells---------------------------------------------------------------------Lan Wei, Halmurat Upur(128)
175. Uighur Medicine Capparis spinosa treatment of 67 Cases of chronic arthritis ------------Obul
Hupurjan(128)
176. Uighur Medicine Karapxa decoction as a hypouricemic agent: reduces serum uric acid level,
inhibits liver xanthine oxidase in hyperuricemic mice model and, scavenge free radicals in
vitro------------------------Nurmuhammat Amat, Palidan Wushuer, Halmurat Upur, et al(129)
177. Kali Zira （Vernonia anthelmintica Willd ）injection induced allergic reaction in 6 cases---R.Umar, J.Abduwayet, Tursun Wuphour, et al(129)
178. Uighur Medicine Xipayi Kui Jie’an effective Gene Expression Profiles on Model of
ulcerative colitis rat colon organization------------Yunusi Ki，Z.Jingping，Y.Mijit，et al(130)
179. Uighur Medicine Xipayi Kui Jie’effect appreciation and apoptosis on HT-29 human colon
cancer cells--------------------------------------------- K.Yunusi，Y.Mijit，Z.JingPing，et al(130)
180. Concept, Classification and Analysis of Uighur Medical toxic drugs in Traditional Uighur
Medicine Hospital of Xinjiang Uighur Autonomous Region -------------------------A.Turdi(131)
181. Clinical effect observation on treatment Adenomyosis with Traditional Uighur
Medicine------------------------------------------------------------------------------------ M.Abliz(132)
The Third Part
Mongolian
Medicine
182. Clinical report of the Mongolian psychosomatic medicine interaction therapy for the
treatment of complex disease --------------Na Gong Bilige，A Runa，Chang Ming，et
al(133)
183. On the Mongolian understanding and dialectical treatment of pigmented purpuric
dermatoses--------------------------------------------------------------------------Chen ShaNa(133)
184. Persistance in the Combination of Scientific Culture and Humanities Culture in the
Modernization of Mongolian Medicine--------------------------------Te Buqin, Bai Changxi(134)
185. The antibacterial and diuresis action of Mongolian medicine “ yunga-4 ”
---------------------------------------------------------------------------------------------------Guqid(134)
186. The sand treatment, Mongolian medicated bath, Mongolian medicine for oral administration:
10
three combined treatments of rheumatoid arthritis-------------------------A la Teng Chu Lu(134)
187. The experience of cervical cancer treatment combined with Mongolian medicine European
medicine-------------------------------------------------------------------------YH Bai, CM Wang(134)
The fourth Part
Integrative
Medicine
188. A Clinical Study on Changes of TCM Syndrome and Relevant Factors in Patients with
Esophageal Carcinoma before and after Radiotherapy------------------Wu Langjie, Du
YeQin(136)
189. Analysis of Bacteria Flora in Nasal Cavity on 102 Cases Acute Asthama-----------Ma Huan,
Li FengSen(136)
190. BiPAP combined with Acupuncture in the treatment of Chronic Obstructive Pulmonary
Disease complicated with Pulmonary Encephalopathy research---------- Wei Chenggong, Fu
Ziyi, Liu Xiaohong, et al(137)
191. Clinical Evaluation and Lung Function of Plastering Therapy of TCM for Chronic
Obstructive Pulmonary Disease in Stable Phase in China: A Systematic Review of
RCTs------------------------------------------------------Li FengSen, Gao Zhen, Jing Jing, et al(138)
192. Comparison between the Mongolian and Western Medical Understandings of the Regularity
of Infectious Disease Course Development ------------------Tu Men Wu Li Ji, Li Guo Hua(138)
193. Correlation between prothrombotic state markers and TCM syndrome type in Uighur and
Han patients with premature coronary heart disease ---------------- L.Hao, Wang XiaoFeng,
Zhai Xueqin, et al(139)
194. Different Syndromes in Patients with Asthma-related Changes in Immune Indexes--------Su
Jun, Li FengSen, Ma Huan, et al(139)
195. Explore the scientific meaning of “Lung Governing Skin and Hair” from SIgA in
secretions of asthma, urticaria patients-----------------------------Zhang YanLi, Li FengSen(140)
196. Intestinal Microflora structure and function analysis in Traditional Uighur Medicine
syndrome of asthma------------------------------------------------Li ChunYan, Halmurat Upur(141)
197. Study on Metabonomics Common Characteristics of COPD about Traditional Chinese
Medicine Kidney Depletion Phlegm and Blood Stasis Syndrome and Uighur Medicine
Abnormal Savda Syndrome-----------------------Xu WeiFang, Halmurat Upur, Li FengSen(141)
198. Observing the clinical efficacy of combined treatment of Traditional Chinese Medicine and
Western medicine to ventilator-associated pneumonia---------Zhang YanPeng, Lv ShuQin(143)
199. Plasma metabonomics study on the asthma diagnosed as Shenxu-tanyu syndrome in
Traditional Chinese Medicine with NMR---------------------Yan XingHai, Halmurat Upur(144)
200. Serum Metabonomics Research on Shenxu-tanyu Syndrome Type 2 Diabetes Mellitus Based
on NMR--------------------------------------------------------------------You Li, Halmurat Upur(145)
201. Studies on Traditional Chinese Medicines----------------------------Brantner Adelheid,
Lower-Nedza Agnieszka, Shi YuJing(145)
202. Study of acupoint application therapy on rat prothrombotic state and establishment of the
corresponding animal model ----------------------------------Zhang Wenhui, Song XiaoPing(146)
203. Study on Cytokine Regulation Mechanism of Downregulating Eosinophils By Tonifying
11
Shen in Traditional Chinese Medicine to Prevent Asthma Relapse-----------------Zhang
WenBin, Mao Bing(147)
204. Study on Esophageal Carcinoma Traditional Uighur Medicine Syndromes and the Change of
T-lymphocyte Subsets and NK cells of Patients Before and After Radiotherapy----------Zhou
Jie, Du YeQin(148)
205. Study on immune and endocrine disturbance of bronchial asthma by Traditional Chinese
Medicine, Uighur Medicine and Western Medicine------------Li FengSen, Halmurat Upur(149)
206. Study on Neuro-Endocrine-Immune disturbance of Type-2 diabetes Millitus by Traditional
Uighur Medicine and Western Medicine--------------Mutallip Obulkasim, Halmurat Upur(151)
207. Study on the Phlegm-Stasis Syndrome in Complex diseases and the Prethrombotic
State------------------------------------------------------------------ S.Abdurhim,Halmurat Upur(153)
208. Study on relationship between TCM syndrome differentiation and NeuroendocrineImmune-Netwok in Type-2 diabetes----------------------Palidan Wushuer, Halmurat Upur(154)
209. Study on the relationship between Qi asthenia phlegm-stasis syndrome of the malignant
tumor and Immunity Cell and Hormone secretion ---------------------------------Wang Qiaolin,
Du Yeqin, Halmurat Upur, et al(154)
210. The clinical observation of the fixation in the treatment of acute ankle sprain in Traditional
Uighur Medicine--------------------------------------------------------------Jin Hao, Lv FaMing(155)
211. The Clinical Research of Acupuncture Combined with Auricular Acupuncture for Patients
with Acute Exacerbation of COPD to Improve their Quality of Life and Pulmonary
Function-----------------------------------------------------------------------Cao Lin, Liu Zhiyan(156)
212. The correlation of TCM syndrome with the BODE index and quality of life for patients with
COPD at stable phase-------------------------------------------------------Zhou Wei, Mao Bing(156)
213. The effect of Tanreqing injection on lipopolysaccharide-induced airway inflammation, mucus
hypersecretion in rats and their own dose-response relationship--------------Zhou YunQing,
Mao Bing(158)
214. The Modern and Traditional Uighur Medicine research of neuro-endocrine-immune in
malignancies------------------------------------------------------------Zhang Li, Halmurat Upur(159)
215. The Observation of Curative on Extracorporeal High-Frequency Hyperthermia Combined
with Yangzhengxiaoji Decoction ’ s Treatment of Advanced Non-small-cell Lung
Cancer----------------------------------------------------------Wu ShanShan, Zhang HongLiang(161)
216. The relationship between Bronchial asthma TCM syndrome differentiation and their NE、
DA、5-HT changes--------------------------------------------------You LiNa, Halmurat Upur(161)
217. The relationship between tumor patients’ plasma monoamine neurotransmitter changes and
their Traditional Uighur Medicine syndromes------------------------------------Chen Qingying,
Du Yeqin, Halmurat Upur, et al(162)
218. The study between IFN-γ methylation and Chinese medicine syndrome type in subsets of
allergic rhinitis patients between Uighur and Han in Ili of Xinjiang-----------------------Zhang
QingQuan, Gao Li(163)
219. The study of the improvement of clinical effect about “Reinforcing Qi and Activating
Blood” in Stable Chronic Obstructive Pulmonary Disease (Qi Deficiency and Blood Stasis
Syndrome)-----------------------------------------------------------------------Min Jie, Mao Bing(164)
220. The Study on Levels of Immunoglobulin and Complement in Henoch-schonlein Purpura
Patient with Different Syndrome----------------------------------Zhang Cheng, Bai Yusheng(165)
12
221. The study on the imbalance of Regulatory T cells/Th17 cells in the different Traditional
Chinese Medicine syndromes of asthma-------------------------------Yan Lu, Zhou Xianmei(166)
222. To study allergic rhinitis of TCM and IL-4 gene methylation between Uighur nationality and
Han people comparative in Yili of Xinjiang---------------------------------Liu FuTai, Gao Li(167)
223. To study the relative of Kidney asthenia blood stasis type deafness and mtDNA 12s rRNA
gene mutation in deafness of Uighur ethnic in Xinjiang region of China------------------------Su
Xuefen, Li Yanhua(167)
224. Oncothermia
combination
with
Traditional
Chinese
Medicine:
network
approach------------------------------------Hegyi Szasz A, G. Andocs, et al(168)
225. Airway inflammation and hypothalamic-pituitary-adrenal axis function in kidney-yang
deficiency type of Chinese medicine in asthma and chronic obstructive pulmonary
disease--------------------------------------------------------- Du Yijie, Li Bei, Cao Yuxue, et al(169)
226. Baicalin restores corticosteroid sensitivity in cigarette smoke induced inflammation by
modulating HDAC2 in vivo and in vitro----------- Li Lulu, Wu JinFeng, Liu BaoJun, et al(170)
227. Establishment and comparison of combining disease and syndrome model of asthma with
‘kidney Yang deficiency’ or ‘abnormal Savda’---------------- Li Bei, Luo QingLi, Mammat
Nurahmat, et al(170)
228. Icariin attenuates LPS-induced acute inflammatory responses: Involvement of PI3K/Akt and
NF-κB signaling pathway------------------- Xu Changqing, Liu BaoJun, Wu JinFeng, et al(173)
229. Icariin inhibits corticosterone-induced apoptosis in hypothalamic neurons via the PI3-K/Akt
signaling pathway--------------------------Zhang Hongying, Liu BaoJun, Wu JinFeng, et al(173)
230. Pharmacokinetics of forsythoside after intravenous administration in beagle dogs--------Shi
Rong, Xuan ZhenYu, Ma YueMing, et al(174)
231. Research on the correlation between expression of foxp3 protein and airway inflammation
and the effects of Icariin in a mouse model of asthma----------------- Jin Hualiang, Li Bei, Luo
QingLi(174)
232. Some Chinese Medicine Components Attenuating Oxidative Stress and Airway Remodeling
in COPD Rat via Keap1-Nrf2-ARE Signaling Pathway-------------------- Lv Yubao, Li Lulu,
Sun Jing, et al(175)
233. The Effects of Icariin, Baicalin on Keap1-Nrf2-ARE Signaling Pathway and Beas-2B Cells
Induced by Cigarette Smoke Extract (CSE)------------- Lv Yubao, Li Lulu, Sun Jing, et al(176)
234. The Effects of some Chinese Medicine Components on Oxidative Stress and Airway
Remodeling of COPD Rats-------------------------------- Lv Yubao, Li Lulu, Sun Jing, et al(177)
235. The research on the relationship between compatibility of Buzhongyiqi Decoction and
immune regulation------------------------------Luo Qingli, Dong JingCheng, Guo Xin, et al(180)
The Fifth Part
Modern
Medicine
236. A comparison of the serum and sputum inflammatory mediator profiles in asthma and COPD
-------------------------------------------------Cao Yuxue, Gong Weiyi, Zhang Hongying, et al(181)
237. A new type health food supplement with analgesic and Anti-inflammatory Activities,
ard-p401, but without adverse reactions ------------Michio Katagi，Akio Yasukawa，Chiaki
13
Kudoh，et al(181)
238. A study of 406 cases with fever in the year between March 2009 and March 2010-----Li
Zheng, Zhang Yanxin, Li FengSen(182)
239. Anti-inflammatory and endocrine-regulated effects of icariin on cigarette smoking induced
chronic obstructive pulmonary disease in rats --------------Zeng LiXuan, Gong Weiyi, Wu
JinFeng, et al(183)
240. Anti-inflammatory effects of icariin on cigarette smoke induced inflammatory responses in
mice-----------------------------------------------------------Li Lulu, Wu JinFeng, Liu BaoJun, (183)
241. Application of the Delphi method of development of chronic obstructive pulmonary disease
"fast"、"slow" criterion--------------------------------Li FengSen, Gao Zhen, Jing Jing, et al(184)
242. Baicalin is anti-inflammatory in cigarette smoke-induced inflammatory models in vivo and in
vitro: A possible role for HDAC2 activity----------Li Lulu, Wu JinFeng, Liu BaoJun et al(184)
243. Calcineurin/NFAT signaling pathway mediates ET-1-induced proliferation by regulating
phosphodiesterase-5 (PDE5) in pulmonary artery smooth muscle cells------------Lu Jiamei,
Xie Xinming, Han Dong, et al(185)
244. Catgut implantation at acupoint on the effects of infertility of PCOS rats
-------------------------------------------------------------------------------Tian Yali, Yin GaiZhen(185)
245. Clinical value of serum procalcitonin and C-reactive protein in diagnosis of bacterial
infections ----------------------------------------------------------Wei Yanrong, Yang Huiqing(186)
246. Comparison of standard sample preparation methods with real time fluorescence quantitative
PCR of Bacteroides thetaiotaomicron in intestinal from db / db type 2 diabetic mellitus
mice-------------------------------------------------------Yi JinYang, Wang Ye, Zhu Ming, et al(187)
247. COPD xide measurement and influencing factors------------------- Wang Nana, Li FengSen,
Bai WenMei, et al(187)
248. Correlation analysis on acute exacerbating COPD patients FENO and pulmonary
function-----------------------------------------------Zhai Yujiang, Li FengSen, Xu Qian, et al(188)
249. CPPE (Compound of Pomegranate Peel Extraction) Reduce Adipogenesis Through Inhibit
Mitotic Clonal Expansion-----------------Wang LiFeng, Tao YiCun, Zhang MinFang, et al(188)
250. Different Type Certificate in Patients with Asthma who Body Fluids, and Peripheral Blood of
the Change of the Relevant Immune Index--------------------------------Su Jun, Li FengSen(189)
251. Discrimination of Type 2 diabetic patients from healthy controls by GC metabonomics
method based on their serum fatty acid profiles-------Ma XiaoLi, Tian Lan, Li linlin, et al(190)
252. Distribution and Prevalence of Human Papillomavirus (HPV) in Uighur Women in China: A
Population-Based Genotype Study-----------------------------------------------L.Hua, G.Abdixkur,
Nurmuhammat Amat, et al(190)
253. Effects of baicalin on airway remodeling in asthmatic mice----------------Sun Jing, Li Lulu,
Wu JinFeng, et al(191)
254. Expression of aquaporin 1 increases proliferation and metastasis potential of lung
cancer----------------------------------------------------------Wei Xiaobai, Dong JingCheng(191)
255. Icariin attenuates airway inflammation and eosinophil infiltration by suppressing eotaxin and
CCR3+ production in acute and chronic mouse models of allergic asthma --------------- Cai
Cui, Du WenJing, Li Lulu, et el(192)
256. Icariin attenuates glucocorticoid insensitivity mediated by repeated psychosocial stress on an
ovalbumin-induced murine model of asthma ---------------------------------------------Li Bei,
14
Duan Xiaohong, Wu JinFeng, et al(193)
257. Icariside II Induces Apoptosis of Melanoma Cells in vivo through the Downregulation of
Survival Pathways--------------------------Wu JinFeng, Xu JinHua, Erika A. Eksioglu, et al(193)
258. Idiopathic acute eosinophilic pneumonia, one case of clinical features of analysis------Zhou
LinFu, Zong XiaoFu (194)
259. Impact of psychosocial stress on airway inflammation and its mechanism in a murine model
of allergic asthma-----------------------------------Li Bei, Duan Xiaohong, Wu Jinfeng, et al(194)
260. Intestinal bacteria support HIV infection？------------------------Shu ZhanJun, Li FengSen,
D.Tuerhong, et al(195)
261. Metabonomic classification and detection of small molecule biomarkers of malignant pleural
effusions----------------------------------------------Zhou XianMei, He CuiCui, YuMei L et al(196)
262. Mitochondrial gene mutations in Uighur patients with type 2 diabetes mellitus in
Xinjiang-------------------------- S.Abuduryim, Nurmuhammat Amat, Halmurat Upur, et al(196)
263. More attention should be paid to mast cells/basophils than to IgE in allergy: proposed
procedure for diagnosing allergic disorders---------------------------------------He Shaoheng(197)
264. Moving and Breathing training on short-term effect of the stable COPD patients-------------Li
FengSen, Li ChunYan, Du Lijuan, et al(198)
265. Neuroprotective effects of icariin on corticosterone-induced apoptosis in primary cultured rat
hippocampal neurons-------------------Liu BaoJun, Zhang Hongying, Xu Changqing, et al(198)
266. Pathogenesis of idiopathic pulmonary fibrosis:from initial apoptosis of epithelial cells to lung
remodeling?------------------------------------------------------Jin Hualiang, Dong JingCheng(199)
267. Preliminary Analysis of the miRNA Expression between Fresh and Old Peripheral
Anticoagulant Blood-------------------------------------- Zhu ManLi, Jiao Yi, Wang Ye, et al(199)
268. .Preliminary Analysis of Transcription Level of FOXOs Genes in Patients’ Feet and Ankles
Who Suffer Xinjiang Uighur Congenital Clubfoot----------------Dong HaoJie, Lv FaMing(199)
269. Prethrombotic State is a common manifestation of chronic disease: A clinical study
------------------------------------------- T.Aji，S.Abudoureyimu, Nurmuhammat Amat, et al(200)
270. Puerarin effect and mechanism of inhibition of airway inflammation and airway remodeling
in chronic asthma model-----------------------Zhou LinFu, Wang Wenlu, Li Hongyan, et al(201)
271. Regulation of STAT signaling in mouse bone marrow derived dendritic cells by respiratory
syncytial virus-------------------------------Jie Zhijun, Darrell Dinwiddie, Albert Senft, et al(202)
272. Research on expression regulation of relevant cytokines of Th17 in COPD Mouse lung
tissue--------------------------------------------------------Wang Jing， Li FengSen，B.Aishan(202)
273. Seeking best menu of walking exercise for a healthy volunteers through the regulation of
peripheral white blood cells in number----------------Nobuo Yamaguchi, Yimit D,
Nurmuhammat Amat, et al(203)
274. Significance And Enlightment Of The Discovery On The Blood, Circulation Driven By
respiration And Of The Study On The Mechanism Of The Day-Sweat And The
Night-Sweat-----------------------------------------------------------------------------Li Zhigang(203)
275. The apigenin raised CD4 + CD25 + Treg cells and inhibit the study of the role of airway
inflammation in acute asthma model------------------------Zhou LinFu, Li Hongyan, Wang
Wenlu, et al(204)
276. The influence of different electroacupuncture strength between inflammatory cells factor and
cerebral edema in cerebral ischemia reperfusion rats----------------Duan Lijun, Li HePing(204)
15
277. The Research of Asthma patients with exhaled nitric oxide and blood eosinophil
levels----------------------------------------------------Yang Weijiang, Wang Nana, Li Fengsen(205)
278. The Study of IL-4 、 IFN- γ Gene Methylation of Allergic Rhinitis in Uygur, Han
people-------------------------------------------------------------------Lou ZhongZhang, Gao Li(206)
279. The Study on the relationship between Cruor-fibrinolysis system and the HuiZhuoTanZu
Syndrome of Coronary Heart Disease------------------------------Xie Xiaoliu, An Dongqing(206)
280. Unique performance like pleural effusion fatal huge mediastinal liposarcoma a
case----------------------------------------------Zhou LinFu, Li Meimei, Zhang Shijiang, et al(207)
281. Winter Disease consumer Clinical evaluation of asthma paste the treatment of chronic
obstructive pulmonary disease---------------Wu Ruohan, Bian YongJun, Li GuangXi, et al(208)
282. Xinjiang Uighur congenital clubfoot genepolymorphism Analysis---------Dong HaoJie, Lv
FaMing(208)
The Sixth Part
Other Medicine
283. Original article Accuracy of 18F-FDG PET/CT for lymph node staging in non-small-cell lung
cancers----------------------------------- Liu BaoJun, Dong JingCheng, Xu Changqing, et al(209)
284. A new trial for relief-oriented design of molecule from natural ginseng other than hot water
extraction--------------------------------------------- Nobuo YAMAGUCHI, Tsugia MURAYAMA,
Masafumi NAKATO, et al(210)
285. Antibacterial Activities of Extracts and Constituents in the Rhizomes of Diploid and
Tetraploid Gingers (Zingiber officinale Roscoe)--------------------------------Patar A, Tanaka N,
Maeda H, et al(210)
286. Biodiversity: A link between traditional medicine and modern medicine-----------------Anake
KIJJOA(211)
287. Characteristics of traditional acupuncture therapies in Japan, Korea and
China---------------------------------------Kenji Kawakita, Yong-Suk Kim, XiaoPin L, et al(211)
288. Current Trends in Medical Education--------------------------------------Muhammad Aslam(212)
289. Education Management in Faculty of Medicine in Kabul Medical University, Kabul,
Afghanistan----------------------------------------------------------------------Samad
Omar(212)
290. Effectiveness of Integrated Medical Curriculum in Pakistan: A Public Medical College
Experience-------Muhammad Saeed, Muhammad Iqbal Khan, Zeiyad Afzal Keyani, et al(213)
291. Folk medicine in Turkey---------------------------K. Hüsnü Can BASER, Ekrem SEZIK(214)
292. Influence of motor activity on health condition of students in Avicenna Tajik State Medical
University----------------------------------------------------------------------------Sagdieva Sh. (214)
293. Innovative Strategies in Medical Education and Challenges faced in Establishing Gulf
Medical University in the Middle East- A Case Study------------------------Vinod Abraham(215)
294. Knowledge, Attitude and practice of medical ethics of faculty of a medical university, in
Karachi, Pakistan----------------Zulfiqar Ali Shaikh, Masood Hameed Khan, Dur Muhammad
Shaikh, et al(215)
295. QOL assessment and proposed scale for evaluation of traditional medicines------------Nobuo
Yamaguchi , Nurmuhammat Amat, Yimid Delitiat, et al(216)
16
296. Search for bioactive compounds to inhibit plant pathogenic organisms--------------Narong
Singbuaraudom(216)
297. Secondary metabolites from a Türkish marine sponges------------------------------Konuklugil,B,
Proksch,P, Gözcelioğlu,B, et al(217)
298. Study of Prevalence of Asthma in male workers of Leather Tanneries in Lahore, Pakistan
----------------------------------------------------------------------------Ali, Sajid, Shehzad, et al(217)
299. Some questions of the development of Uzbek public medicine and practical aspects of it on
the public health of the population-----------------------------------------------------Khamraev(218)
300. YNSA as a new acupuncture treatment for pain killing---------------------Hegyi Gabriella(218)
301. Prevalence of gout in Pakistan-------------------------------------------------------------- Sajid(219)
302. Traditional Uighur Medicine theory of "KuWat" (energy) classification and its function
---------------------------------------------------------------------------------Tursun T，Kawuz R(219)
303. Experience of «RAWAN» application at sick children with bronchial asthma in
Turkmenista--------------------------------------------------------------- Dmytriy Sebrebryakov(220)
304. Clinical effecacy observation Uighur Medicine Preparetion Denar on the patients with viral
hepatitis with Cholestatic syndrome----------------------------------- Dmytriy Sebrebryakov(221)
305. Epidemiological analysis of vitiligo according to traditional Uyghur medical theory and
western medicine----------------------------------------------------------Tuohuti K, Shawuer R(221)
17
A-001
Overview of Traditional Chinese Medicine
Dong Jingcheng 1*
1Huashan
Hospital, Fudan University, Institute of Traditional Chinese and modern medicine, 200040, Shanghai, China;
*Correspondence should be addressed to E-mail: jcdong2004@126.com
1. Traditional Medicine: Traditional Medicine refers to a wide range of medical systems developed
independently before modern medicine. It is a part different from the mainstream of modern medicine:
confronted medicine. The definition issued by World Health Organization is: medicine that includes
treatment, diagnosis and prevention of diseases or maintenance of body healthy, making use of drugs
based on plants, animals and minerals and one or a variety of methods including psychotherapy, body
therapies and other means developed in practice. Many ancient civilizations and peoples in the world
have their own traditional medicines. 2. Traditional Chinese Medicine: Traditional Chinese medicine
(TCM) is a medical system with unique theories that accumulated, summarized and gradually formed in
the long-term medical care and life practice in China. Traditional Chinese medicine collectively refers to
medicines of all nationalities in China, including national medicine such as Han medicine, Tibetan
medicine, Mongolian medicine, Uygur medicine. In all traditional Chinese medicines, due to Han’s largest
population, earliest text history, longer cultural record, abundant clinical practice and perfect traditional
medical theory system, the influence of the Han medicine is most significant in China as well as in the
world. After the 19th century, Western medicine was introduced to China and got popularity. Han
medicine was called ‘Chinese medicine’, different from the ‘Western’ modern medicine. 3. General
situation of various traditional medicines in China: 1) Traditional Chinese medicine collectively refers to as
the traditional medicine of all nationalities in China, including the medicine of the Han medicine, Uygur
medicine, Tibetan medicine, Mongolian medicine, Dai medicine, Hui medicine, Miao medicine, etc. They
are often self-contained, characteristic and profound. Han Chinese medicine (TCM) is found on
theoretical basis such as yin and yang theory and five elements theory and physiological and pathological
basis such as organs and meridians, focusing on the overall concept and syndrome differentiation,
diagnosing based on the combination of observation, listening, interrogation, and pulse-taking. TCM is
also created eight methods of treatments, namely, sweating, vomiting, purgation, harmonizing, warming,
clearing heat, dispersing masses, medicating according to prescription principles involving monarch,
minister, assistant and guide. The theoretical basis of Uighur medicine is regarded as temperament of
different body fluids. Uighur medicine has a deep understanding of human anatomy. In addition to the
great attention of four diagnostic methods, the urine also set diagnostic methods for urine diagnosis,
observation of stool and sputum diagnosis except for inspection. Tibetan medicine also considers yin and
yang theory and five Elements theory as a theoretical basis. In addition to the four diagnostic methods, it
focuses on urine diagnosis and is characteristic of syndrome differentiation. Tibetan medicine composes
prescriptions according to character, taste and function of drugs and character and taste after digestion.
Mongolian medicine is based on yin and yang, five elements and five yuan theories, and has a more
profound understanding of human anatomy. In addition to commonly used observation, listening,
interrogation, and pulse-taking, Mongolian medicine also focuses on other diagnostic methods such as
press and olfaction as well as urine diagnosis and differentiation of cold and heat. Dai medicine has a
thorough understanding of body structure. It pays attention to the differentiation of four elements and five
skandhas and the differentiation of three pan with delicate principals and fruitful methods. Dai medicine
1
contains simple, small and generous prescriptions. Hui medicine is dominated by harmony between
human being and nature and the idea of organic whole, and based on primordial qi, yin and yang and
seven elements doctrines. It is predominant of differentiation of physiques, combined with differentiation
of syndrome, disease and meridian. Hui medicine pays attention to syndrome differentiation, and
therapeutic methods are flexible and abundant. Miao medicine classifies all diseases as two categories:
cold and fever. Thermal treatment is used in cold disease and cooling treatment is used in fever disease.
The methods of treatment are very rich. The characteristics of prescription are single distribution and
trinity. Zhuang Medicine is oriented by yin and yang theory and regards three air synchronization view as
the theoretical basis. It emphasizes on eye diagnosis, focusing on a combination of differentiation of
disease and syndrome, attaching great importance to the use of animal drugs. The major traditional
medicines are compared with respect to conception, formation time, philosophical foundation,
representative celebrities, representative works, anatomy, physiology, disease name, etiology,
pathogenesis, diagnosis, treatment principle, treatment method, prescription, medication characteristics,
and it is easy to find the great similarity among them. 2) Traditional Chinese medicine is composed of the
three parts: Chinese medicine, ethnic medicine and folk herbs. Before Opium War, Chinese medicine has
always been the mainstream medicine in Chinese society. Both commonalities and differences exist in
Chinese medicine and other national medicine. Ethnic medicines are independently creative and
self-contained in their own culture, and they form their system respectively with characteristics. China has
55 minorities. From the ancient to the modern society, they have numerous creations in medication and
only parts of them have been spread till now. Due to the uneven development of various ethnic medicines,
there is significant difference in the ability of inheritance and propagation among them. Therefore, to the
present, only Uighur, Tibetan, Mongolian, Dai, Miao medicine have established their teaching and
research system. Understanding and application of traditional Chinese medicine is based on Chinese
medicine theory, and has a unique theoretical system. The core of Chinese medicine theory is the nature
of herbs, including four natures, five favors, channel tropism, ascending and descending, toxicity, etc.
Similar to traditional Chinese medicine, Uighur, Tibetan, Mongolian, Dai, Miao and other ethnic medicines
also have their own medicinal theory system. Uighur Medicine has ‘Alcorn doctrine’, ‘Mi Zha Ji Doctrine’,
including their drug doctrines of herbs, animal drugs and mineral drugs. Character and taste of these
herbs are divided into dry, hot, wet, cold and hot and dry, hot and humid, wet and cold, dry and cold, and
1, 2, 3, 4 classes. According to their nature, Tibetan medicine is divided into ‘hot’ and ‘cold’ categories,
with six flavors, eight natures and seventeen kinds of effects. Tibetan medicine is able to treat 20 kinds of
diseases. The basic nature and flavor of Mongolian medicine is sweet, sour, salty, bitter, acrid and
astringent. According to ascending and descending, and formed herbal theory, eight effects of Mongolian
medicine are summarized, including heavy, greasy, cold, blunt, light, blunt, hot and sharp, known as
flavors of eight effects. Dai medicine contains doctrine of four elements and five skandhas. Internal
treatment of Miao medicine emphasizes cold medicine for fever disease, hot medicine for cold disease,
and reinforcing methods for deficiency syndrome. Flavors of fragrant, spicy, sweet, tingling are attributed
to thermal drugs, and tastes of acid, pungent and astringent are ascription to cold medicine. Prescription
is based on clinical experience. In different ethnic medicinal systems, there are similarities and
differences for the same herb. In addition, long-term used medicinal plants in some minorities are named
by their local names. 3) In summary, we find that: traditional medicines of all nationalities in China are full
of intensive theory, flexibly diagnostic and therapeutic techniques, elegant prescription and medication.
They have their own characteristics and systems, and make a great contribution for keeping Chinese
prosperity. But we should also recognize that course of development of Chinese traditional medicines is
2
quite different from modern medicine, it is always around the center of diagnosis, treatment and solution
to practical problems and collects all the wisdom. By means of ancient plain materialist philosophy,
operational and practical medical system are established. The core of the system is ‘organs theory’, ‘body
fluid theory’, ‘temperament theory’, ‘material theory’ and etc. This system is full of technical science and
empirical science, representing the highest establishment of experimental medicine in the world, and it is
the result of the collective efforts made by Chinese ancestors in the field of traditional medicine. In this
process, it is difficult to find out the hint of theoretical science, basic medical research and their
contribution. A variety of traditional medicine build theory centered with viscera and body fluids, which
reflected that people's understanding to human life are still at an early stage. In the view of theoretical
value, there is little gap between viscera theory and body fluids theory. Both belong to the simple
phenomenological theory, and have simple whole view, balanced view, etiological view, pathological view
and preventive view. However, viscera theory is more specific and abundant than humoral theory. Just
because of this, viscera theory is more practical than humoral theory. Viscera theory correlates the
function of viscera and human external variables through intermediate variables such as gas, blood,
sperm, body fluid, and ultimately reached by the state that inferring external manifestation by means of
changing viscera function or identifying viscera function via external manifestation. Not only that, the
determination of herbal efficacy are also included into unified system centered with viscera theory, making
reason, method, compound and drug integrated. Since Chinese traditional medicine represented by
Chinese medicine formed, experienced medicine in clinic was developed instead of experimental
medicine and basic medicine; however, it has reached the highest realm of human experienced medicine.
Some of these valuable experiences have been proved consistent with the knowledge of modern
medicine; and some experiences are likely to contain certain future direction of the development of
modern medicine; it is without doubt that valuable experiences born thousands of years ago have their
temporal and cultural limitations. In this medical system, in addition to valuable experiences, there are a
lot of understanding with temporal and cultural imprint. For example, in 1870, before human knew the
relationship between microbes and diseases, all infection and diapyesis phenomenon will be generally
described as ‘toxins’, ‘miasma’, ‘hostility’ and etc.; human’s corresponding disease resistance mechanism
such as immune mechanism is generally referred to ‘righteousness’. Western historians LH·Banar said
the following statement for the medical advances before 1878: ‘The medical science had not yet been
prepared at that time. A large part of medical practice was medical technology and treatment of the
disease was to reduce the symptoms rather than correct the pathological alternation. Laxative,
bloodletting, extract, tincture and multi-drug mixture, the compositions in many parts appears to be only a
little or no pharmacological effect from the current point of view, but they were general treatments at that
time. For general health measures of disease prevention, they were just beginning to be noticed.’
3
A-002
The Basic Structure of Traditional Chinese Medicine
Dong Jingcheng 1*
1Huashan
Hospital, Fudan University, Institute of Traditional Chinese and modern medicine, 200040, Shanghai, China;
*Correspondence should be addressed to E-mail: jcdong2004@126.com
1. Introduction: As mentioned earlier, the essence of the traditional Chinese medicine has always been
most advanced experienced medicine in the world. Its vast system is full of practical medical technology
and experiences that gradually recognized by modern medicine; it contains the germ that indicates the
direction of future development of human medicine; still, it is widespread to find the simple understanding
of temporal and special cultural imprint. Therefore, generally speaking, the basic structure of traditional
Chinese medicine may be mainly composed of the following three parts: the part that is consistent with
modern medicine, the part that is beyond modern medicine unconsciously and the part that needs to be
re-recognized or abandoned. 2. The part that is consistent with modern medicine: In the 16th century, the
so-called ‘western medicine’ was introduced to China, but had little effect. After the Opium War, ‘western
medicine’ began to develop in China, and therefore in the history of Chinese civilization before the Opium
War, traditional Chinese medicine had always been the leading force of medical care in the Chinese
nation. The key of successfully medical are lies in its efficacy. ‘Excellence is from experience’. A lot of
practice and experiences construct a solid foundation for its theory. After the foundation of new China, the
development of ‘western medicine’ in China has entered into a new stage. With the increasingly
deepened impact of modern medicine, especially the development of ‘integrative medicine’ from the end
of 1950's, some theories, therapeutic principle, technology, prescriptions of modern life science were
elaborated and the basic structure of traditional Chinese medicine also became clearly. One of the most
important parts is the part that has reached a consensus with modern medicine, and that correlated with
many aspects of basis and clinic in traditional Chinese medicine. 3. The part ahead of modern medicine
unconsciously: Chinese traditional medicine is a practice medicine built on the basis of experiences. The
theoretical system is built by means of ancient plain materialist philosophy, mostly by the method of
‘syndrome differenciation’, use of natural means to treat illness rather than emphasize on consistency
with contemporary science and technology. Unlike modern medicine, the view that ‘Nature that cannot be
certified cannot be conquered’, keeping abreast of requirements of contemporary science and
technology, makes traditional Chinese medicine unconsciously have the super-awareness of the era in
addition to the overall features of the old and simple, that is, the so-called "integrative research is able to
continue to reveal the essence. 4. Some parts which need to be newly recognized or abandoned: In the
system of human science, including medical science, the conception becomes from new to old while its
correction becomes from absolute to relative, and the application extension becomes from large to small.
It is the inevitable regulation of development. All the concepts and rules indicate the stable aspect of
object. However, this kind of stableness is conditional, partial and relative while unstableness is absolute
and unconditional. Any scientific systems should confess their demerits and rectify them so as to make
new concepts, methods and theory. Meanwhile, modern medicine and Chinese Traditional Medicine were
founded in some simple recognition and culture and specific era. So TCM theory should be divided into
two parts, one of which need to be promoted, the other of which need to be abandoned. Integrative
medicine is an important way to achieve it.
4
A-003
The Future of Traditional Chinese Medicine
Dong Jingcheng 1*
1Huashan
Hospital, Fudan University, Institute of Traditional Chinese and modern medicine, 200040, Shanghai, China;
*Correspondence should be addressed to E-mail: jcdong2004@126.com
We should scientificly and rationally recognize TCM, including its theory, experience, technology, method
and prescription, define its merits and demerits by using modern technology and method like epidemic
method and medical certificate method so as to further confirm scientificalness and efficacy. We should
define the mechanism and material basis of modern science and develop the comparison of similar
theory, technology, method and prescription, valuing survival of the fittest to make TCM pith outstanding.
In addition, when study traditional Chinese medicine, especially drugs, we should pay attention to its basic
ideas and be guided by these ideas, which will help the modernized development of traditional Chinese
medicine. A large number of clinical evidence suggests that traditional Chinese medicine is of lower
efficacy and potency than modern chemical drugs. One of the reasons for this phenomenon may be a
departure from basic theories of traditional medicine in researching drugs, such as the whole idea;
currently researches on traditional medicines are mostly carried out under the guidance of the reductionist
thinking used in modern medicine. Take anti-arrhythmic drugs as an example, researches based on
reductionist thinking indicate that Chinese medicine monomers such as berberine are of lower efficacy
than modern drugs such as amiodarone in the treatment of arrhythmia. But under the guidance of the
whole idea, the above-mentioned anti-arrhythmic medicine monomers are not only the best target of the
disease, but have multi-target effects and intervene at least two ion channels. Clinically, if multiple
monomers intervene with reasonably compatibility, satisfactory effects will be gotten and with no obvious
side effects. However, treatment of synthetic drug with single component has an average effect but
obvious side effects. For example, quinidine is a kind of arrhythmia drugs in modern medicine, but
quinidine may induce Q-T interval syndrome and arrhythmia because it inhibits a certain target strongly.
Thus, in order to develop safe and efficient antiarrhythmic drugs, we should study compounds, effective,
distinguished and experienced prescriptions, analyze the effective components, and find out the best
compatibility acted on multiple targets. In this way, we are able to develop new drugs at the component
level. Essence speaking, the characteristic of traditional medicine is wholism which means the synthetic
action of multi-elements, multi-targets, multi-angle and multi-layers to adjust the treatment of diseases.
The basic medicine mechanism should be the basic point of synthetic action. When each herbal medicine
in the prescription is clear, the effective combination to human should be studied so that the syndrome in
TCM could be made clear. In other words, medicine tests syndrome. Some famous medicines of modern
medicine come from ancient natural plants, whose developing experience should be a good way of many
effective traditional medicines. The true value of integrative medicine and modernized traditional
medicine is not the foundation of new theories, but the mechanisms of those effective traditional
medicines, which enriches the culture of modern medicine. And it perhaps the historic mission of TCM
and integrative medicine.
‘……There is a incline that we can forget the close combination of whole science and human being, forget
those advanced and profound scientific findings. Leaving them alone is just meaningless.’
——Oven﹒
Schrodinger
5
A-004
The Literature Research of Ming Dynasty Physicians on Treating the Syndrome of Chest
Pain
Li Fangshuai1, An Dongqing1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should
be addressed to andongqing3@gmail.com
Objective: Collecting and collating the main medical writings about chest pain’s etiology，pathogenesis
and prescriptions in Ming Dynasty, discussing the understanding of the etiology and pathogenesis when
Ming Dynasty physicians treating chest pain, summarizing the characteristics and laws of using medicine
of this period in order to better serve and clinical work. Methods: 1.The method of traditional philology,
mathematical statistics and using the prescription to prove syndrome, to discuss the law of prescription for
treating chest pain from relevant books written by 22 doctors in Ming Dynasty. And select the etiology and
pathogenesis of Wang Kentang, Liu Chun, Qin Jingming works for chest pain, prescription summary, the
induction of a common illness machines and the law. Results: The traditional Chinese medicine
treatment of chest pain from Ming Dynasty physician: use 142 kinds of medicine totally, cumulative 954
times less frequent dosing. Most of the herbs are for tonifying deficiency, moving blood, warming organs
and smoothing qi, the cumulative frequency is 64.89%; the frequency of using it is more than 1%, the 24
taste of commonly used herb is licorice, orange peel, ginseng, cinnamon, Wulingzhi etc, the cumulative
frequency is 55.45%; Prescription herbs of Siqi is mainly 68% warm and calm; bitter, acrid, sweet of
Wuwei mainly accounted for 95.8%; Spleen, heart and liver meridian is mainly 61%; Frequency greater
than 1% of the 24 herbal medicine in the cluster analysis, automatic formation of the seven cluster
combinations. 2.Three on behalf of the physicians (Wang ken tang, Liu Chun, Qin jing ming) write on
Chest Pain etiology of pathogenic cold invasion and occupation (32.31%), physical weakness (20%), due
to pathogenic heat (18.46%), emotional chastity and eating disorders (10.77%). Medication usage: the
prescription of tonifying deficiency, moving blood, warming organs and smoothing qi, the most commonly
used cumulative frequency is 55.15%. There is no significant difference in Wuwei and meridian belongs of
herb using from three doctors prescription, there are some differences in the use of four character herbs.
Conclusion: (1) The prescription law of Ming Dynasty physicians is: ①
Commonly used herbs is for
tonifying deficiency, moving blood, warming organs and smoothing qi, ②
Besides commonly used
supplementary herbs such as licorice and ginger, Wulingzhi and ginseng as main and effective herb on
behalf of the physicians in the Ming Dynasty to treat chest pain, ③
Siqi is mainly warm and calm, bitter,
acrid, sweet of Wuwei accounting for the main, the main meridian are spleen, heart and liver meridian; (2)
The method of using the prescription to prove syndrome is mainly qi and yin deficiency, blood stagnation
syndrome, qi stagnation and cardiothoracic, cold stagnation in systolic, which is commonly seen. (3) the
physician of the Ming Dynasty believe that the chest pain is related to cold evil invasion, physical
weakness, heat evil, emotional chastity, eating disorders, exerting internal injuries and other factors, the
main pathogenesis is systolic Blockage.
6
A-005
The Preliminary Studies on Herbal Medicine Resources of Aconitum from Ghulja and
Easten Region in Xinjiang
Liu Shumin1, Nie Jihong1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to xjnjh411@163.com
Objective: Initially to investigate the species distribution, habitat and reserve situation of Xinjiang
Aconitum plant. To detect the determination of Xinjiang Aconitum Plant Alkaloids and diester alkaloid
content, to certify the resources of the chemical composition. Adopting TLC fingerprinting method,
preliminaryly to carry out the similarity comparison, cluster analysis and principal component analysis of
Aconitum plants in Xinjiang. Initially to select the species of potential medicinal value and similarity with
the existing national legal herbs standard Aconitum carmichaelii Debx. And Aconitum kusnezoffii Reichb,
which is widely distributed and abundantly reserved form Xinjiang Aconitum species. To provide scientific
basis for rational using and development of Aconitum species. Methods: Reviewed literature and
specimens, surveyed species distribution, habitat and status of reserves of Xinjiang Aconitum plant.
Taking Aconitine as reference, determined the total alkaloid content in the Xinjiang Aconitum plants by UV
spectropHotometers with acid dyes. Determined aconitine hypaconitine Aconitine in the Xinjiang
Aconitum plants by HPLC. Using high performance thin layer silica gel G plate, taking n-hexane - ethyl
acetate-methanol(6.4:3.6:1) for dilute potassium iodide, show color with thin bismuth iodide solution of
potassium, got thin layer of fingerprint with color, to generate common pattern via the chemical fingerprint
analysis system software, and carryed out similarity analysis, cluster analysis and principal component
analysis. Results: Initially found five kinds of Xinjiang Aconitum medicinal plants, of which Aconitum
leucostomum Worosch. and Aconitum soongaricum Stapf. in Ghulja were rich, and worthy of further
development and utilization. UV spectropHotometric determined the aconitine between the concentration
range of 0.01176~0.04704 mg/mL had a good linear relationship, the regression equation A=16.484
C+0.0592，
r =0.9992. Average recovery was 100.19%, RSD=1.33%. Sample assay results showed that
total alkaloid content of the Xinjiang Aconitum species is similar with the existing legal herbs standard
Aconitum carmichaelii Debx. and Aconitum kusnezoffii Reichb., it was worthy of further studying. By
HPLC mesaconitine had a good linear relationship (r =0.9999) between 0.0022~0.0864 mg/mL, the
average recovery was 99.69%, RSD=1.04%; aconitine had a good linear relationship (r=0.9999) between
0.0201~2.0120 mg/mL, average recovery was 100.06%, RSD=0.60%; hypaconitine had a good linear
relationship (r=0.9999) between 0.0021~0.0840 mg/mL, the average recovery was 99.68%, RSD=1.08%.
Sample assay results showed that Xinjiang Aconitum plants contained diester alkaloids, but the content
and ingredients were different between different kinds. Screened and optimized the conditions for thin
layer chromatograpHy, spots were clear and separation was good; similarity analysis showed that
Xinjiang Aconitum had some differences in chemical composition between different plant species, which
can be broadly divided into three categories. Among which aconitum leucostomum Worosch. and
Aconitum apetalum(Huth) B. Fedtsch. Had high similarity with Aconitum carmichaelii Debx. And Aconitum
kusnezoffii Reichb. Conclusion: Xinjiang Aconitum species had high medicinal value, and deserved for
further studied. UV spectropHotometry was simple, accurate, and can be used for the determination of
total alkaloids from Xinjiang Aconitum plants. HPLC method was simple, accurate, high sensitivity, can be
used in determination of the diester alkaloids from Xinjiang Aconitum plants. Established thin-layer
chromatograpHic fingerprints laid the methodological foundation for the study of the fingerprint of Xinjiang
Aconitum plants. Initially selected the species of Aconitum leucostomum Worosch. and Aconitum
soongaricum Stapf., which were widely distributed and abundantly reserved form Xinjiang Aconitum
species, and had potential medicinal value and similar with the existed national legal herbs standard,
were worthy of further being studied.
7
A-006
A Systematic Review on Zhisousan Treatment of Infections
Li Fengsen 1*, Jing Jing1, Li Chunyan1, Gao Zhen 1,2
1
Affiliated Traditional Chinese Medicine Hospital，Xinjiang Medical University& National Clinical Research Base of Traditional
Chinese Medicine, 830000, Urumqi, Xinjiang, China; 2Xinjiang medical university, Urumqi, 830011, Xinjiang, China;
*Correspondence should be addressed to Fengsen602@163.com
Objective: A systematic review on Zhisousan treatment of infections. Methods: Meta-analysis of
randomized controlled trial (RCT) cough Decoction combined with Western medicine to treat the infection,
to adopt RevMan5.1 software for statistical analysis. Results: A total of 24 published RCT (n = 2 274), but
the prevalence of lack of methodological; Cough simply scattered its addition and subtraction with
western medicine is efficient compared to fixed effects models show that combined OR = 7.90, 95% CI
[5.67,11.01], P <0.00001; Zhisousan and Decoction combined with Western medicine. western medicine
is efficient compared to fixed effects models show that the merger, OR = 3.62,95% CI [2.22,5.92], P
<0.00001. Conclusion: Zhisousan Treating infections cough may be effective, but because research and
test methodology flawed, and there is publication bias, the findings still can not fully sure, we still need
high-quality research trials further confirmed.
A-007
The clinical study on Side-lying position and shaking method in treating Cervical
Spondylotic Radiculopathy of 120 Cases
Liu Junchang1, Muhetaer Ayoufu1, Wang Xinjun1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to wxj6283@sina.com
Objective: To compare the curative effects of cervical spondylosis after treatment by “Side-lying position
bowed their heads and shaking method”. Methods: 120 subjects were randomized into two groups:
treatment group in which 60 cases were treated by Side-lying position bowed their heads and shaking
method and control group in which 60 cases were treated by Seat inclined pull method, once every other
day were treated 10 times. The clinical symptoms and their scores were observed. Results: After
treatment, the clinical symptoms were all improving in both groups(P＜
0.01),with better effects in treatment
group than in control group in improving neck and shoulder pain and cervical movement（
P＜
0.05）
.
Conclusion: The “Side-lying position bowed their heads and shaking method” is quite effetive for cervical
Spondylotic radiculopathy, and is worth promoting.
A-008
Body Constitution Classification (BCC) of Coronary Heart Disease (CHD) in some parts of
Xinjiang
Li Hui1, An Dongqing1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should
be addressed to andongqing3@gmail.com
8
Objective: to explore the east, south, north of body constitution features of TCM patients with coronary
artery disease, to get coronary artery disease and providing the theoretic basis. Methods: From October
2009 to October 2010, using cross-sectional survey method, in the east, south, north the selected five
municipal districts and 10 hospitals with coronary heart disease was the first collected by western
medicine diagnosis and diagnosis of coronary heart disease and TCM diagnosis for chest discomfort in
660 examples, using standardized 9 kinds of TCM physique scale in patients with coronary artery
disease, TCM identification from the physical implementation of gender, age, regional, national, body
mass index, syndrome type, coronary lesions count explore characteristics. Results: (1) the east, south,
north of TCM type of constitution in patients with coronary artery disease and clip physique, false or true
inclusion type of constitution is given priority to, Xinjiang patients with coronary artery disease common
TCM type of constitution for qualitative, phlegmy wet qi difficiency and blood stasis qualitative;
qualitative；
(2) different sexes, different age, body mass index, national physique different
characteristics；
(3) shouts turbidity phlegm resistance syndrome patients with and clip biased physique,
false or true constitution is given priority to, with the inclusion uncleanness turbidity phlegm resistance
syndrome patients constitution comparison was statistically significant (P<0.05)；
(4) this survey altogether
collected 660 of coronary heart disease patients, choose a case of coronary angiography results of total
249, each type of constitution in coronary lesions narrow count comparison, was not statistically
significant (P>0.05), shouts turbidity phlegm resistance syndrome group with the uncleanness turbidity
phlegm resistance syndrome group coronary lesions count comparison, multivessel disease comparison
was statistically significant (P<0.05).Conclusion: this study found that patients with coronary artery
disease TCM type of body constitution in xinjiang with qualitative, phlegmy wet qi difficiency and blood
stasis qualitative qualitative, in each type of constitution proportion in patients with coronary artery
disease is higher, it is main type of constitution, and concurrently clip physique, false or true mingled with
physical phenomenon exists generally, Xinjiang patients with coronary artery disease syndrome
differentiations with shouts turbidity phlegm resistance syndrome is given priority to, deficiency of
qualitative, phlegmy wet qualitative, blood stasis quality physique patients are predisposed to coronary
heart disease and easier to develop into shouts turbidity phlegm resistance syndrome, Different sexes,
ages, regional, national, weight, syndrome type, degree of coronary lesions TCM body characteristics
different, and the results indicated that the above three physical may in coronary heart disease
occurrence, development exists some internal relevance.
A-009
Case-control Study of the Association between Northwest Dryness Syndrome and
Chronic gastritis
Zhang Huitian1, Zhou Mingxin1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to zmx9998@ vip.sina. com
Objective: To investigate the Northwest Dryness Syndrome (NDS) and chronic gastritis, starting from the
NDS to provide prevention and treatment of chronic gastritis according to the Xinjiang region. Methods:
Epidemiological case-control study by modern methods, Questionnaires as the main form，
according to
the theory of traditional Chinese medicine syndromes of disease and its comprehensive analysis of the
9
performance. Results:(1)Compared with other parts of the country, Xinjiang chronic gastritis symptoms
and syndrome type has its own characteristics;(2) Case group NDS attack rate was 37.86%, in the control
group NDS attack rate was14.29%, the difference was statistically significant (P <0.01);(3) Case group
and control group compare points of NDS, Lung , skin and opening of the body syndrome , deficiencies of
essence and blood of the liver and t he kidney syndrome ,yin deficiencies of the heart and kidney
syndrome, yin deficiencies of the lien and stomach syndrome, and endogenous dampness syndrome, the
differences were statistically significant (P <0.05); (4) Dryness in the case group and non-NDS group set
TCM syndromes compared, Fan Wei liver, spleen Deficiency, damp heat resistance, yin deficiency of
stomach, diet Shang Wei and cold Fan Wei card, the difference was statistically significant (P <0.05);(5)
The total deficiency on the incidence, the dry card cases and cases of non-dry group, dryness control
group, non-dry the control group, statistically significant differences (P <0.05); Comparison of the total
deficiency points, Dryness cases and cases of non-dry group, NDS of case group and control group, NDS
case group and control group of non-dry, non-dry case group and control group of non-dry, dryness
control group and the control group of non-dry, the difference was statistically significant (P <0.05).
Conclusion: The impact of the NDS, the Xinjiang region is different from other regions of chronic gastritis,
has its own characteristics; Patients with chronic gastritis and the prevalence NDS card points are higher
than normal intelligence; Patients with chronic gastritis were diagnosed NDS risk of illness than those who
have none. To the total deficiency, the chronic gastritis combined deficiency in patients with NDS and gas,
blood, yin, yang permit incidence dryness were higher than those who suffer and have more disease who
suffer from more non-NDS Serious.
A-010
The Effect of dipeptidyl peptidase IV in 3 extracts of plants from Xinjiang
Yang Zhenzhen1, Wang Xuehua2, Wang Ye1, Mao Xinmin3, Han Xue1, Yi Jinyang1, Wang Lifeng4, Ma
Xiaoli5, Li Linlin1*
1Department
of Pharmacology, Xinjiang Medical University, Urumqi 830011, China; Traditional Chinese Medicine Institute,
Urumqi 830011, China;
4
Department of Physiology, Xinjiang Medical University, Urumqi 830011, China;
Analysis and Testing Center Xinjiang Medical University, Urumqi 830011, China;
2Xinyuan
5
Department of
People's Hospital of Xinjing Yili
835800, China; * Correspondence should be addressed to yz-lll@vip.sina.com
Objective: To study the effect of dipeptidyl peptidase IV in extracts from Trigonella foenum-graecum L.
Cicer arietinum L. Punica Granatum L. Methods: The inhibitory effects of these 3 extracts to dipeptidyl
peptidase IV activity were investigated by in vitro enzymatic method and IIe-Pro-IIe was used as positive
reference substance. Comparison of 3 kinds of water extracts of IC50. Results: IIe-pro-IIe IC50 is 0.01
mg/mL. Trigonella foenum-graecum L. Water extraction IC50 is 0.03mg/mL, Punica Granatum L. water
extraction IC50 is 0.19 mg/mL, Cicer arietinum L. water extraction IC50 is 0.09 mg/mL.Conclusion: The 3
extracts of Xin Jiang antidiabetic plant showed obviously inhibitory action against dipeptidyl peptidase IV
activity. Trigonella、
Pomegranate husk、
Cicer arinum L. water extraction to dipeptidyl peptidase IV have
different degrees of inhibition.
10
A-011
The Effects of TianXiangDan on Blood Lipid and Vascular Endothelial Growth Factor in
the Arteriosclerosis in ApoE (-/-) gene Knockout Mice
Jiao Yujie1, An Dongqing1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to andongqing3@gmail.com
Objective: To observe the TianXiangDan on apolipoprotein E gene knockout (ApoE (-/-)) mouse in
atherosclerosis (Arteriosclerosis AS) of vascular endothelial growth factor (vascular endothelial growth
factor ,VEGF)and blood lipid effects. Method：
Sixty Eight-week old ApoE(-/-)gene knockout mice were
randomly devided into 6 groups : control group(fed with 0.9%NACL0.2ml·d-1·10g-1，
n=10),model
group(fed with0.9%NACL 0.2ml·d-1·10g-1，
n=10),TianXiangDan (TXD) high dose group(fed with
TianXiangDan 0.36g/mL,n=10), TianXiangDan (TXD) medium dose group(fed with TianXiangDan
0.18g/mL,n=10), low dose group(fed with TianXiangDan 0.09g/mL,n=10), and Atorvastatin Calcium
Tablets (ATV) group (fed with ATV 0.2mg/mL,n=10). The mice were sacrificed after 12 weeks．
The lesion
of atherosclerosis was observed by microscope and pathological staining，
respectively. The Plasma level
of blood lipid was measured by test kit, the level of VEGF was measured by ELISA kit.
Results：
microscope and hematoxylinenosin staining showed that the area of atherosclerotic lesion was
significantly less in TXD-treated groups than in model group(P<0.05),especially TXD high dose group
was the lest.The result of aortic arch stained showed lipid deposition was less in TXD-treated groups mice
than in the model group(P<0.05), also especially TXD high dose group was the lest. Moreover，
the level
of VEGF was lower in TXD-treated groups mice than in the model group(P<0.05)．
also especially TXD
high dose group was lowest. Conclusion：
TianXiangDan could reduce the levels of serum total
cholesterol (Serum total cholesterol TC), triglyceride(Triglyceride TG) and low-density lipoprotein
cholesterol(Low density lipoprotein LDL-C) on apolipoprotein E-knockout mice . TianXiangDan could
reduce plaque of peripheral atherosclerosis in VEGF expression and the area of AS plaque effects.
A-012
Primary Study on Chemical Composition and Biological Activity of Arctium tomentosum
Mill
Zhang Haoke1, Tian Shuge1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to tianshuge@hotmail.com
Objective: By studying on chemical composition and its antioxidant, antibacterial effect, to provide
research data pharmacological effect further study and reasonable exploitation and utilization of Arctium
tomentosum Mill. Methods: (1) Study the extraction process of arctiin in Arctium tomentosum Mill.
through extraction method, extraction solvent and its consumption separately, then choose the best
extraction process. (2) Determine arctiin and arctigenin in different extraction parts by HPLC to define
enrichment parts of arctiin. (3) Separate and purify the ethanol extract to get arctiin monomer. (4) Apply
two antioxidant models (remove DPPH free radicals, the vitality effect superoxide anion) to select
antioxidant activity from different polar extraction parts of ethanol extractions. (5) Use 96-well plates to
11
select antibacterial activity from different polar extraction parts of ethanol extractions. Results: (1) The
best extraction process of arctiin Arctium tomentosum Mill. using 70% ethanol reflux extraction for 1h, 8
times solvent and extract 3 times. (2) The root of Arctium tomentosum Mill. contain a little arctiin and no
arctigenin; compared with other parts dry and mature seeds contain more arctiin and arctigenin; there are
little arctigenin but no arctiin in leaf and flower; the stem and petiole contain almost no arctiin and
arctigenin. (3) Study the chemical composition of Arctium tomentosum Mill. by
spectroscopy(1H-NMR、
13C-NMR、
IR、
UV) and define it is arctiin. (4) Antioxidant activity experiment
proved that ethyl acetate extraction part has good antioxidant activity. (5) Butanol extraction part has good
activity of antibacterial and inhibit the growth of bacterial biofilm. Conclusions: (1) The ethanol extraction
parts of Arctium tomentosum Mill mainly contains lignans and high content of arctiin. (2) Established the
methods of extraction, separation, purification for Arctium tomentosum Mill. which are simple and stable.
(3) Ethyl acetate extraction part has good antioxidant activity. (4) Butanol extraction part has good
antibacterial activity.
A-013
The Study on the Quantities Chemical Composition and Chemical Pattern Recognition in
Coriopsis Ticntoria from Different Areas
Sun Zizeng1, Zhang Yanli1, Aytulun Simayil1*
1Pharmacuetical
Institute, Xinjiang Medical University, Urumqi 830011, China; *Correspondence should be addressed to Simayil
A
Objective: To study the character of the contents and distribution of chemical composition of Uygur
Medicine Coreopsis ticntoria flowers (CTF), and to provide valuable experimental evidence for the quality
evaluation and control of CTF. Method: Ten batches of samples，
CTF．
coming from different producing
areas of Xinjiang were carried out to quantitate by UV and ICP．
Principal component analysis and
Hierarchical clustering analysis were applied to the study on the chemical components which were
extracted from the original data. The principal component composition and chemical pattern
recognition．
Result: Three principal analysis results showed that Cu, Zn，
Fe，
Mn，
Ni，
Cr and Al may be the
characteristic elements. The ten samples could be clustered reasonably into three groups, and the
contents of chemical composition of CTF were related to the ecological environment, such as temperature
and location．
Conclusion: This paper, by measuring the number of content indicators, using principal
component analysis and cluster analysis clearly reveals the regularity of the different origins and
differences in CTF．
A-014
Regulation Effect of OboBiyanChongji on Expression of IL-2, IFN-γ, IL-13 and Nasal
Mucosa in the Experimental Allergic Rhinitis in Rats
Jiang Liming1, An Yang1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to anyang13899989606@yahoo.com.cn
Objective: To investigate the effect of OboBiyanChongji for allergic rhinitis (AR) rats on IL-2, IFN-γ, IL-13,
12
IgE levels and nasal mucosa, and compare OboBiyanChongji groups with XinQin particles groups to
indentify the mechanism of pharmacodynamic action and theropy best dose. Method: Egg albumin
sensitz- ation made rats animal model of allergic rhinitis. The model successfully rats were treated with
different doses of OboBiyanChongji, saline, Xin Qin particles, which are compared with each other. The
method of ELISA determine in serum the total IL-2, IFN–γ, IL-13 and IgE level. Using Giemsa staining for
detection of nasal mucosal eosinophilia. Calculate the nasal mucosa and submucosal eosinophilic
number by using microscope. Results: The results of serum IL-2, IFN-γ of high dose group are
significantly different with the model group, low dose group , middle dose group, nasal eosinophilic
expression (P<0.05). The levels of IL-13, IgE and nasal mucosal eosinophilic expression decreased
(P<0.05). The high dose group and blank group are relative to plasma in IFN-γ, IL-2, IL-13, IgE and
eosinophilic expression differ significantly (P<0.05). But they has no statistical significance with medium
dose group (P>0.05). Conclusion: High dose of OboBiyanChongji can effectively improve the allergic
rhinitis in rat plasma IL-2, IFN-γ levels, and decrease IL-13, IgE and nasal mucosal eosinophil levels. The
high dose group of OboBiyanChongji is more effective than low doses, middle group and XinQin particle
group.
A-015
Research on the Changes of Different Contentsin Fried Aconitum pendulum Busch
Wu Lin1, Zhang Fan1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to zhangfan596@163.com
Objectives: To analyze the influences on contents of toxic and active compounds in Aconitum pendulum
Busch caused by different fried methods which were used by different national medicines, and to provide
scientific basis for searching out the frying mechanism and enhance the quality of medicines. Methods:
Several Aconitum pendulum Busch were fried by different special methods used in Tibetan medicine,
Qiang medicine and Hui medicine. The total alkaloids in all fried products were isolated by ultrasonic wave
method and HPLC method and ester alkaloid in all fried sample. ICP-AES, AAS and AFS were the first
methods to be used to scan and analyze macro elements, trace elements and heavy metals in Aconitum
pendulum Busch different fried samples, which were correlated with people’s health and lives. Results:
All the contents of total alkaloid and ester alkaloid in Aconitum pendulum Busch samples decreased after
fried. The method of frying with water could drop the toxicity largely, and the method of frying with Qingke
wine may retain the activity of the medicine. All the heating methods were the deeply influencing to
aconitum alkaloid. Also, it was found to be the content of Hg rose greatly in the experiment. Conclusion:
Different methods of frying would influence the contents in Aconitum pendulum Busch.
A-016
Research on Xinjiang Region Physical Types of Patients with Hyperuricemia on behalf of
Teaand Chinese Medicine Intervention
Li Li1, An Dongqing1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to andongqing3@gmail.com
13
Objective: The incidence of hyperuricemia in recent years was significantly elevated, and concomitant
cardiovascular disease are obviously increased, the onset of many influence factors of hyperuricemia and
cardiovascular diseases, the relationship and related mechanism is complicated, many academic point of
view remains controversial, this research through Xinjiang patients with traditional Chinese medicine
constitution identification, discuss its physical type distribution clinical observation of traditional Chinese
medicine, and through the generation of tea on patients with hyperuricemia uric acid, lipid metabolism
index, renal excretory function and tissue plasminogen inhibitor effect of Chinese medicine on behalf of
the tea, mechanism of action, for the early treatment of Chinese herbal medicine of hyperuricemia and
concurrent disease and provide a theoretical basis. Methods: using the standard 9 kinds of TCM
constitution classification in scale to 1400 patients with hyperuricemia patients constitution identification,
create database. And choose from 70 patients with blood stasis syndrome type of phlegm dampness
constitution hyperuricemia patients, randomized control principle is divided into treatment group and
control group, two groups were given diet and exercise intervention, treatment of traditional Chinese
medicine intervention group to make tea, observation curative effects after the treatment of integral, and
blood uric acid (SUA) , lipid metabolism indices, renal excretory function and tissue plasminogen inhibitor
(PAI-1) was analyzed by comparative analysis. Results: (1)Patients with hyperuricemia biased
constitution mainly by the phlegm wet, damp heat and blood stasis quality 3 kinds of physical endowment.
In the improvement of traditional Chinese medicine, traditional Chinese medicine on behalf of tea group
superior efficacy to exercise control group (P<0.05) ; (2) two groups of intervention can significantly
decrease the SUA (P<0.01); (3) improvement in TC,TG, traditional Chinese medicine on behalf of tea
group was significantly better than the exercise control group (P<0. 01) ; (4) Two groups of PAI-1 more
before treatment significantly (P < 0.01). Conclusion: Xinjiang region high uric acid of hematic disease
patient biased constitution mainly phlegmy wet quality, hot and humid quality and blood stasis quality
mainly three kinds of type of constitution, at the same time, the mountain tea can greatly improve phlegmy
wet clip stasis syndrome in patients with high uric acid constitution disease symptoms of traditional
Chinese medicine, can promote uric acid eduction, lipid metabolism disorders of has a therapeutic effect,
after treatment, the organization type fibrinolytic enzyme inhibitors of the original (PAI-1)have declined
obviously, and show that the tea mountain of high uric acid and related disease concurrent disease have
certain preventive therapy effect.
A-017
Safety Evaluation of Awei Capsule: Research of Acute and Subchronic Toxicity in rodent
Han Rong1, Xue Jie2*, Nurmuhammat Amat3*
1Second
2Research Department, Xinjiang Medical
Affiliation Hospital of Xinjiang Medical University, Urumqi , 830011, PRC；
3Traditional Uighur Medicine Institute, Xinjiang Medical University, 393 Medical University
University, Urumqi, 830011, PRC；
Road, 830011, Urumqi , Xinjiang Uighur Autonomous Region, China；
*Correspondence should be addressed to
kycxuejie@163.com(Xuejie), nur818@hotmail.com.( Nurmuhammat.Amat)
Objective：
Awei Capsule (AC) which is a medicinal herb formula has been used in traditional Chinese
medicine in the treratment of diseases including and diabetes mellitus. However, there is very little
information regarding the toxicity of CA to give an assurance of safety for clinical treatment. To provide
safety information for CA, we evaluated the acute and chronic toxicity of the AC in rodent. Methods:For
14
the acute study, ICR mice was administered in single doses of 56.9g/kg of AC given by gavage. Also
determined the maximum tolerated dose (MTD) was the AC which was administered to ICR mice at doses
of 56.9g /kg twice a day. General behavior adverse effects and mortality were determined for up to 14
days. In the sub-chronic dose study, the AC was administered rectally at doses of 2.3, 15.76 and 25.6g/kg
daily for 180 consecutive days and the toxic effects were assessed using biochemical and haematological
data and histology of vital organs. Results: We found no mortality and no abnormalities in clinical signs,
body weights, and necropsy findings for any of the animals in the acute and subchronic studies. AC may
not have any single-dose toxicity;the MTD of CA was over 9.888g/kg, and it is safe for mice.
Conclusion:The results indicate that AC do not posses any significant acute toxicity in mice and chronic
toxicity in rats.
A-018
Sophora alopecuroides extract on human coloncancer cell line HCT116 strains of chick
embryo chorioallantoic membrane experimental study on tumor angiogenesis
Li JingJie1, Lv Shuqin 1, 2*
1
Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University；2Traditional Chinese Medicine Institute, Xinjiang
Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should be addressed to lvshuqin@126.com
Objective: Through the establishment of the people HCT116 colon cancer cell lines chicken embryos
allantoic membrane transplantation tumor model, to observe Xinjiang bitter beans extract huai set after
transplantation of alkali role of anti-angiogenic phenomenon, and observe xinjiang bitter beans thick total
alkaloid in the role of colon cancer cells HCT116 people antiproliferative and induce the apoptosis of
function, this paper discusses the xinjiang bitter beans antitumor, and the mechanism of action of
angiogenesis. Method: By MTT method, flow cytometry detection of sophora alopecuroides Xinjiang
was rough total alkaloid against human colon cancer cell proliferation HCT116 and induce the apoptosis
role, and observe chicken embryos fluffy allantoic membrane transplantation tumor angiogenesis
changes, with the positive buy fluorescent microscope the ratio of blood vessels; Immunohistochemical
method to determine the chicken embryos fluffy allantoic membrane transplantation tumors VEGF dyeing
strength and the change of MVD. Result: Xinjiang bitter beans thick total alkaloid colon cancer cells to
HCT116 role 24, 48 h IC50 alue concentration were 24 mg/ml, 6.82 mg/ml. Xinjiang bitter beans thick total
alkaloid can reduce people HCT116 colon cancer cell cycle G1 phase percentage, heighten S phase and
can induce people HCT116 colon cancer cell apoptosis. Bitter beans extract set in the role of CAM tree
alkali medicine group than blood vessels after area (VA/CAM) were less than model control group (P <
0.05), and at the same time, reduce tree set of the tumor, reduce the expression of VEGF
microessel-density (P < 0.05). Conclusion: Xinjiang bitter beans thick total alkaloid can restrain people
HCT116 colon cancer cell proliferation, and induce the apoptosis, interfere with the cell cycle G1 and S
phase, which block the tumor cell mitosis. Bitter beans extract set in the role of alkali tree chicken embryos
fluffy allantoic membrane transplantation tumor has obvious antiangiogenic effect, its mechanism may
reduce the expression with tumors VEGF and reduce microessel-density relevant.
15
A-019
Study on the difference between Uygur ethnic group and Han people who suffered from
mild cognitive impairment (MCI) with electroacupuncture therapy
Guo Hui1, Liu Zhiyan1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to xjliuzhiyan@yahoo.com.cn
With the help of 1H-MRS to observe the clinical effect of electroacupuncture on mild cognitive impairment
of the Uygur ethnic group and Han people, we researched into the diversity of nation differences between
Uygur ethnic group and Han people of MCI, meanwhile, find the effective way to treat MCI by traditional
Uygur Medicine. Divided 22 patients into Han people group (13 cases) and Uygur ethnic group (9 cases)
according to the nation, based on the principle“tonifying kidney、
replenishing essence and benefiting
marrow, strengthing brain and benefiting mind , Take Bai hui、
Feng chi、
Xuan zhong、
Fu liu、
San yin jiao、
Tai
xi as the main acupoints, all of them received electroacupuncture, observe the change of MMSE、
MOCA
assess chart and two groups’ NAA/Cr、
Cho/Cr before and after the treatment by 1H-MRS. There is an
obvious difference on left side hippocampus (NAA/Cr) between Uygur ethnic group and Han people group
compared with the treatment before. And the MMSE and MOCA changed obviously after three courses
treatment compared with the treatment before (P<0.01)；
NAA/Cr of Uygur ethnic group and Han people
group’s left side hippocampus increased compared with the treatment before (P<0.01), Cho/Cr of Han
people group’s left side hippocampus decreased compared with the treatment before (P<0.01), there is
no obvious change on Uygur ethnic group ’s left side hippocampus (P>0.05). Electroacupuncture can
improve the general cognitive function of mild cognitive impairment (MCI) patients; there may be nation
diversity of Uygur ethnic group and Han people group mild cognitive impairment patients in the partial
brain metabolite. The two groups patient’s NAA/Cr increased after treatment, Electroacupuncture could
improve cognitive function and defer senility through repair nerve cell. The Han groups’ Cho/Cr decreased
after treatment, Electroacupuncture could improve cognitive function and defer senility through restrain
the form of neurospongium.
A-020
Study on the Pharmacognosy, Chemistry and Acute Toxicity of Aconitum Soongaricum
Stapf
Cai Dongmei1, Zhang Fan1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to zhangfan596@163.com
Objective: To study the pharmacognosy of Aconitum soongaricum Stapf systematically; to confirm the
components and to study the acute toxicity of Aconitum soongaricum Stapf on mice, while investigating
the acute toxicity, toxic signs, organ coefficient, median lethal dose (LD50) and 95% confidence interval
on mice of A. soongaricum Stapf. Methods: Macroscopic and microscopic identifications were carried out
to observe the crude drug, fabric and powder, and high performance liquid chromatography (HPLC) and
thin layer chromatograph (TLC) were used to analyze the alkaloids in A. soongaricum Stapf, and
corresponding methods were set up to determine the contents of the alkaloids. Different concentrations of
16
A. soongaricum Stapf original medicinal powder suspension are given to mice, for observing and
recording the activity and toxicity in mice, and at the same time, for computing the value of LD50. Results:
The characteristics of A. soongaricum Stapf were spindle root, 2-4 roots, black brown skin and bluish
violet sepals. The components of A. soongaricum Stapf were 12-epi-napelline, songorine, neoline,
songoramine and napelline, and also, songoramine and napelline were found in it for the first time. The
mice showed reduced activity, sweating, tremor and mouth breathing toxic symptoms after medicine
administration about thirty min, and the peak mortality rate was two hours. The median lethal dose was
155 mg/kg, and the 95% confidence interval was 137-175 mg/kg. Conclusion: The results can be taken
as the reference for identifying the species, analyzing the quality and laying down quality standard of the
crude drug.
A-021
Study on The Best Extraction With AngeLica From Knee and ArthraLgia HerbaL Paste
S.Yun 1, G.Liang 1, W. Xinjun 1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to wxj6283@sina.com
Objective: Study on the best extraction with angeLica from Knee and arthralgia herbaL paste. Methods:
The extraction technique was designed by orthogonal design, the content of Ferules acid as indicators,
exploring the influence of factors such as ethanol concentration, ethanol volume, extraction time and so
on. Results: Extraction of ferules acid in Angelica the optimum condition was: 50% to 7 times the
amount of ethanol refluxing extract three times, 2.5h each time. Conclusion:The repeated experimental
results are stable, and the method is feasible.
A-022
TCM Hung Drag line Drainage is the Treatment of High Anal Fistula
Chang Zhongsheng 1, Yu Dandan1
1
Affiliated Traditional Chinese Medicine Hospital of Xinjiang Medical University First, 830054, Urumqi, China
Objective: Explore Chinese medicine dragging line method for high anal fistula. In order to explore a
more ideal operation method, we use Chinese medicine dragging line method about the treatment of 40
patients with high complexity of anal. Effectively improved the curative rate, Reduce the recurrence,
panful, and shorten the course of treatment. Compared for the traditional hang line method, Chinese
medicine dragging line method treatments of 40 patients with high complexity of anal were all cured. They
anal physiological function, Position are all normal, no significantly damaged, no mucous leakage, no
anal incontinence and form anal fistula ect. Conclusion: Chinese medicine dragging line method is an
effective method for high anal fistula, it’s easy to operate, more security, and effective in preventing the
occurrence of anal.
17
A-023
The Effect of Sophora Alopecuroides Extract to the Receptor Tyrosine Kinases in
Colorectal Cancer Cell Lines
Jiang Qin 1, lv ShuQin 1,2*
1
Affilated Traditional Chinese Medicine Institute, Xinjiang Medical University；2Traditional Chinese Medicine Institute, Xinjiang
Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should be addressed to lvshuqin@126.com
Objective: To investigate the effects of the extract from Sophora on anti-proliferative of human colon
cancer cell line HCT116, to further improve the treatment of colorectal cancer in TCM provide
experimental evidence for the efficacy. Methods: Using tetrazolium blue (MTT) method, add in the final
concentration of 0.6 mg/mL, 0.9 mg/mL, 1.2 mg/mL, 1.5 mg/mL, 1.8 mg/mL, 2.1mg/mL the extract from
Sophora and blank medium, Calculate the cell growth inhibition rate and IC50, Through Flow cytometry
Sophora extract on human colon cancer HCT116 cell growth inhibition and anti-proliferative effect.
Western Blot to find EphA1 protein expression detection. The treatment group were treated with the
Concentration of IC50, cells were collected at 48 and 72h,respectively.After antibody incubated in the
imaging system to detect protein bands,Gray-scale analysis of protein bands using the Quantity one
software,And statistical analysis. Results: Experiments shows, after treatment with different
concentrations, sophora extract reversed its effect on HCT116 significantly (P<0.05), the IC50 is
1.2mg/ml and have a good dose-effect relationship, FCM detection showed sophora extract could induce
apoptosis of HCT116 cell. The apoptosis rate of HCT116 cell was significantly augmented while the
concentration of sophora extract increased. Protein first increased with the prolongation of the extract
from Sophora,then the trend of the expression reduced. There was a significant difference between them.
Conclusion: Chinese medicine Sophora extract can efficiently inhibit the proliferation and induce
apoptosis of HCT116 cell and have acertain extent to EphA1 protein expression.
A-024
The Clinical Observation of Tianxiangdan Capsule in Treating Angina Pectoris in
Coronary Heart Disease (Syndrome of Qi Deficiency and Blood Stasis)
Song Xiaopeng 1, An Dongqing 1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to andongqing3@gmail.com
Objective: To observe the efficacy of Tianxiangdan Capsule in treating coronary heart disease (CHD)
angina and on serum lipids. Method: One hundred and six patients with stable angina pectoris type in
CHD with the syndrome of Qi deficiency and blood stasis were selected and divided randomly into three
groups: Treatment group (n=37) treated with Tianxiangdan Capsule;Control group I (n=36) treated with
Tianxiangdan Particles;Control group II (n=33) treated with Tongxinluo Capsule;Four weeks as one
course. The clinical efficacy of the three groups were observed and their effects on blood lipids. Results:
(1) The significant efficiency about the improvement of angina: in treatment group was 83.79%,Control
group I was 86.11%, Control group II was81.82 %. The efficacy of the three groups were no significant
difference (P>0.05).(2) The significant efficiency about the improvement of Traditional Chinese Medicine
Syndrome: in Treatment group was 86. 48%, Control group I was 88.89%, Control group II was84.85%.
18
The three group efficacy had no significant difference (P>0.05).(3) The efficient about the improvement of
ECG: in Treatment group was 70.27%, Control group I was 72.22%, Control group II was69. 70%. The
three group efficacy had no significant difference (P> 0.05) . (4) Stopping or reducing rate of the
Nitroglycerin tablets: in Treatment group was 78. 37%, Control group I was 75. 00%, Control group II
was75. 76%.The difference was not statistically significant (P>0.05).(5) Lipids before and after treatment
compared to the situation: Three lipid check, Before and after treatment, there was statistically significant
difference (P<0.05 or P<0.01) . Treatment group of the TG improvement was better than the Control
group II (p<0.05). (6) Coagulation before and after treatment compared: Three coagulation check, Before
and after treatment, there was statistically significant difference (P<0.05 or P<0.01). After treatment
between the three groups, The difference was not statistically significant (P>0.05). (7) Security analysis:
The three groups of patients during treatment were not found adverse effects, safety index examination
also found no obvious abnormalities. Conclusion: Treatment of angina pectoris with syndrome of Qi
deficiency and blood stasis by Tianxiangdan capsule was safe and effective.
A-025
The Experience of Using Dihuang Pills to Treat the Chronic Cough and Asthma Treatment
of Lung
Shi Suofang1
1First Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu, 210029, China
Pulmonary chronic cough with dyspnea , often touching refractory, has a final effect on kidny；
kidney
deficiency can always occur ultimately, thus has more opportunities to apply modified rehmanniae
decocction. Based on clinical practice，
the author elaborated the application about the syndromes of
pulmonary chronic cough with dyspnea with the use of modified rehmanniae decoction, and introduced
modified application ways of using it from following descriptions：
Qiwei Douqi Wan，
which can reinforce
kidney to accept Qi and astringe the lung to relieve asthma；
modified Liuwei Dihuang Wan and Baihe Gujin
Tang，
which can replenish the kidney to nourish yin and moisten lung for arresting cough；
modified Liuwei
Dihuang Wan and Jinshui Liujun Jian，
which can tonify kidney to relieve phlegm and relieve cough with
dyspnea；
modified Zhibo Dihuang Wan and Guizhi Jia Longgu Muli Tang，
which can nourish kidney to fall
fire and regulate Yin and Yang；
modified Jinkui Shenqi Wan and Zhenwu Tang，
which can warm Yang to
transform Qi and reinforce kidney to promote diuresis。
It is thought modified rehmanniae decoction can
treat the pulmonary chronic intricate and refractory diseases, having higher value of enlightenment in
clinic.
A-026
The influence of p38 MAPK pathway to asthmatic guinea pigs which made by Qu
Fengxuanbi
Shi Suofang 1
1First
Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu, 210029, China
Objective: To discuss the functional mechanism of Qu Fengxuanbi (remove wind and apoplexy) Formula
(Pingxiao Heji) on bronchia asthma. Method: Make bronchia asthma animal model with guinea pig
19
sensitized by egg albumen, treat it with Qufeng Xuanbi Formula, detect the phosphorylated p38 protein
expressionin in guinea pig lung tissue homogenates using immunohistochemical and Western-blot
methods；
Results: the two methods showed p38 MAPK phosphorylation of protein levels in the model
group had significantly increased compared with the levels in dexamethasone group, Chinese medicine in
high-dose and low-dose group，
and protein levels in high -dose group below the low-dose group (P<0.05);
Conclusion: Qufeng Xuanbi Formula is effective in the treatment of asthma，
which is closely related with
the inhibition of p38 MAPK pathway expression , and its inhibitory effect is dose dependent.
A-027
The Studies of Regulating Blood Lipid and Antioxidant of Awei Capsules on Hyperlipemia
Rats
Cui Liang 1, Xue Jie 1,2*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, 2Research Department ，Xinjiang Medical University，
Urumqi 830011, Xinjiang, China;* Correspondence should be addressed to kjkxuejie@163.com
Objective: To observe the effect of Awei capsules on regulating the blood lipid, prevention of fatty liver
and improving the function of liver, improving the antioxidant capacity on experimental hyperlipidemia
rats. Methods: Thirty SD rats were randomly divided into five groups: control group, model group and
Awei capsules high-, medium- and low-dose group. Except the control group, other groups were fed with
fat-rich forage. Hgih-, medium- and Low- dose groups were given aqueous solution of the sodium
carboxymethyl cellulose of Awei capsules contents. It were 1.28, 0.64, 0.32g/kg respectively. The rats of
control group and model group were taken aqueous solution of the sodium carboxymethyl cellulose. It
were seven weeks continuously, six days a week. Record the water intake, food intake of rats daily, and
weigh weight of rats weekly. Rats were not given any food, but water for 24 hours before taken the blood.
Rats were injected 10% chloral hydrate to anesthesia. Taken blood from the abdominal aorta of rats till to
euthanasia. Separate serum. Taken the part of liver tissue into 10% neutral Formalin solution for
microscopic detection, and took part of liver saved into the liquid nitrogen for completing the study of
HMG-CoA RNA expression levels by electrophoresis and fluorescence quantitative PCR. The indicators
of detecting: water intake, food intake, rats weight, liver weight, brain weight, heart weight, organ
coefficient; liver appearance and character of the pathologic morphology of liver; TC, TG, HDL-C, LDL-C,
AST, ALT, GLU, NO, MDA, activity of SOD in the serum; and the expression level of HMG-CoA in rat’s
liver. Results: Compared with the blank group, the model group of male and female rats liver color was
yellower; it could be observed lipid droplets and inflammatory cell infiltration phenomenon on liver cells by
optical microscope detection; the difference of liver coefficient, TC, LDL-C, ALT, MDA, NO, SOD activity
and AST female TG was statistically significant(P<0.05,P<0.01). Liver coefficient,TC, TG, LDL-C, AST,
ALT, MDA and NO of model group were higher, SOD activity was lower. Compared with the model group,
the intracellular lipid droplets and inflammatory cell infiltration phenomenon of liver were lower in Awei
capsules three dose groups by the optical microscopy; liver coefficient of three dose groups on female
rats, TC of female rats in three dose groups, TG of male and female rats in high dose group, LDL-C of
male and female rats in three dose groups, AST of female rats in three dose groups, ALT of female rats in
high dose group, ALT of male rats in high dose group, MDA in three dose groups, NO in high dose group,
SOD in high- and middle- dose groups, the differences of them had significant difference ( P < 0.05, P <
0.01). The differences of other index, had no statistical significance. The other indexs of Awei capsules
20
groups have the trend to chosing to the control group. Conclusion: Blood lipids and liver morphology,
microscopic structure, function in hyperlipemia rats were abnormal in model group, so the hyperlipidemia
model of rats was successful. Awei capsules can regulate blood lipid, increase the antioxidant capacity,
prevent fatty liver and improve liver function. But in this study, it was not showed that the intensity of effect
had correlation trend with the dose of Awei capsules.
A-028
The study of quality standards and determination of validity period of Awei capsules
Hou Mingqi 1, Xue Jie 1, 2*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, 2Research Department ，Xinjiang Medical University，
Urumqi 830011, Xinjiang, China;* Correspondence should be addressed to kjkxuejie@163.com
Objective: In order to use the drug safely and effectively, we establish the quality standards and predict
the validity period of A Wei Capsules. Methods:①
Rhizoma Bistortae、
Cortex Magnoliae
Officinalis、
Rhizoma Imperatae were identified by Thin Layer Chromatograph；
②
The contents of Gallic Acid
、
Magnolol and Honokiol were determined by High Performance Liquid Chromatograph. ③
Put the sample in
different humidity、
examine the level of absorbing water and get critical relative humidity. ④
Classical
constant temperature test: put capsules at different temperature of 80℃
、
90℃
、
100℃,examine sample at
different time, the change of different indexes content at different time was determined to estimate the
validity period of the capsules. ⑤
According the items of China Pharmacopoeia ,we determine the content
of water、
loaded quantity differences 、
crumbling time limit and microbial limits. Results:①
Rhizoma
Bistortae、
Cortex Magnoliae Officinalis、
Rhizoma Imperatae have same colour spots on the same location
according the reference substance and the negative samples have no interference. ②
Determined by High
Performance Liquid Chromatograph,the content of GallicAcid was 1.081±0.064mg/g, The contents of
Magnolol and Honokiol were respectively 14.56±0.058mg/g、
6.15±0.036mg/g. ③
The critical relative
humidity was 72%,It presented that we should control surroundings humidity under72% when producing
and storaging drug . ④
The activate energies of different indexes was calculated based on Arrhinius
formula and the validity period was predicted 2.75 years for Magnolol, 1.77 years for Honokiol. ⑤
We
should control the content of water was under 7%, the methods of crumbling time limit were adding 700ml
water at 37℃
,loaded quantity differences and microbial limits were done by the methods of China
Pharmacopoeia. Conclusion: Through multicomponent andmulti-index, we establish the standard of A
Wei Capsules. We can predict the validity period of capsules with the index of contents. All of these can
provide the experiment evidence of evaluating drug quality and storage times.
A-029
The Study on Medicinal Value and Reduce the Toxicity with Processed of Aconitum
soongaricum Stapf in Xinjiang
Wu Chao1, Jiang Lin1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to jianglinjj@126.com
Objective: To research the period of time processed in Aconitum soongaricum Stapf in Xinjiang alkaloid
21
content and the change of the toxicity and pharmacological, determine the Aconitum soongaricum Stapf
in Xinjiang processed in method. Methods: 1.Used by reveed-phase HPLC before and after processed in
Aconitum soongaricum Stapf in Xinjiang alkaloid content changes. 2. By using mice following
intraperitoneal injection method for determination LD50 of different processed products of Aconitum
soongaricum Stapf. 3. Used radiant heat stimulation and acetic acid induced writhing test and
investigated analgesic function of different processed products of Aconitum soongaricum Stapf. 4.
Observed the different processed products of Aconitum soongaricum Stapf to AA anti-inflammatory effect
of rats. 5. To observe the different processed products of Aconitum soongaricum Stapf to CIA
anti-inflammatory effect of rats. Results: 1. Aconitum soongaricum Stapf in Xinjiang was the aconitine
content was higher , the content of Mesaconitine and Hypaconitine was low, processed products of
double ester type of alkaloid content was reduced, single ester type alkaloid content increased. 2. The
toxicity order of different processed products of Aconitum soongaricum Stapf: Product > Literature
method > Pharmacopoeia method (2010) >Kazakh method; The result showed that the LD50 were 3.079
2 g/kg, 26.219 1 g/kg, 10.587 3 g/kg and 39.870 2 g/kg respectively. 3. (1) Compared with contrast group,
The administration groups are able to significantly extend mice from radiation caused by thermal
stimulation of mouse tail-flick latency except the low dose from Pharmacopoeia method (2010) and
Kazakh method; (2) Compared with contrast group, The administration groups could reduced rat torsion
times (P<0.01or P<0.05), which had analgesic effects except the low dose from Kazakh method. 4. (1)
The swelling degree and arthritis index of Aconitum soongaricum Stapf and all of its processed products
groups were lower (P<0.01or P<0.05). (2)The content of Cort and IL -1β in serum of positive
group，
Aconitum soongaricum Stapf and all of its processed products groups were lower (P<0.01or
P<0.05). 5. (1) The swelling degree of Aconitum soongaricum and all of its processed products groups
were reduced. (2)By light microscopy observation, the treatment group synovial histopathological form
had improved significantly. (3)Compared with model group, give medicine 6 weeks, the content of SA,IL-2
and TNF-α with The administration groups had significant differences(P<0.01or P<0.05) except its Kazak
method processed product group. Conclusion: (1)Aconitum soongaricum Stapf processing synergia
from action mechanism and the diester-type alkaloids content reduced , single ester type alkaloid content
increased related.(2) Acute toxicity and pharmacodynamics experiment shows that literature method
processed products and pharmacopoeia method (2010) processed products of analgesia and
anti-inflammatory effects are much better than Kazakh method processed products, But pharmacopoeia
method(2010) processed products of toxic smaller than literature processed products.(3)Aconitum
soongaricum Stapf after processing can reduce the toxicity and increase effect, has some of the medical
values, The pharmacopoeia method(2010) processed products of acute toxicity, pharmacodynamics are
better than the other two kinds of processing methods, So optimization pharmacopoeia method(2010) as
Aconitum soongaricum Stapf reduce the toxicity and increase effect of the processing technology.
A-030
The study on sustained-release technology of Tianxiangdan capsules
Wang Jia1, Jiang Lin1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to jianglinjj@126.com
Objective: To study the preparation technique of Tianxiangdan sustanined-release capsules, and
22
evaluate its release characteristics in vivo and vitro. Methods: 1) On the foundation of extraction
technology in Tianxiangdan capsules, instant-release granules were prepared by wet granulation. First,
using disintegration time as a evaluation parameter, we inspected single accessory of the capsule, then
through the method of orthogonal test design get the best mixed proportion of excipients. 2) Selected
inclusion method of volatile oil to define the best inclusion method, the ratio and yield of the oil in inclusion
complex was used to evaluate the technology based on the orthogonal design, in which the proportion
between β-cyclodextrin and volatile oil, the proportion between β-cyclodextrin and water and rubbing time
were considered to be 3 main factors. 3) The sustained release granules were prepared by solid
dispersion technique, to optimized select the best mixed proportion of sustained release excipients by
uniform design experiment. Investigated every released conditions and established standard of release
degree in vitro, selected the best mixed proportion of instant-release granules and sustained release
granules. 4) The cumulative release percentage in vitro of sustanined-release capsules was determined
by rotating basket method, and discussed released mechanism of the drug. 5) According to determination
of hemodynamic parameters in anesthetized rats and the experiment that is affected of rat models on
myocardial ischemia by ISO, preliminary explored the pharmacokinetics of sustained-release preparation
in body. Results: 1) The best mixed proportion of instant-release excipients that MCC : CMS-Na : L-HPC
is 1:2:3. 2) The optimal inclusion procedure is as follows：
the proportion of volatile oil and β-cyclodextrin
was 1:6 , the proportion of water and β-cyclodextrin is 5:1 and rubbing for 10 min. 3) The best mixed
proportion is EC: HPMC: PVP = 17:2:1 in sustained release granules and finally making 60%
instant-release granules and 40% sustained release granules are mixed in capsules, the drug release
pattern is conformed to the first order kinetics[r1=0.9927、
r2=0.9511]. 4) Tianxiangdan sustained-release
preparation group compared with the ordinary preparation group that LVSP and + dp / dt had statistical
differences (△P <0.05), it is show that the improvement effect of hemodynamics parameters in the
sustained-release preparation group is better than the ordinary ,which are myocardial ischemiahe rats.
Conclusions: 1) The preparation method for Tianxiangdan sustanined-release capsules reported in this
paper is reasonable and feasible. 2) The prepared sustained-release capsules had a better
sustained-release effect,the drug was released stability, and achieved the goal of sustained-release in the
drugs.
A-031
Theory of Acupuncture "reinforcing" and "reducing"
Cai Hua1, Yin Gaizhen2*
1Lanzhou
Military Region, Urumqi General Hospital Department of Rehabilitation Medicine, Urumqi, 830000, China; 2Xinjiang
Medical University School of Medicine Department of Acupuncture basis, 830054, Urumqi, China;* Correspondence should be
addressed to 78701921@qq.com
Acupuncture reinforcing-reducing means that acupuncture treatment can directly play an important role
in the traditional acupuncture attention. Acupuncture reinforcing-reducing can directly original from the
theory of 《
Huangdi Neijing》
, long-term guiding clinically therapeutic and stimulate quantity. For the later a
variety of physical stimulation therapy to lay the foundation. This article from the theory of acupuncture
reinforcing-reducing can direct the origin of the concept, this paper discusses the basis of reinforcing and
reducing. 1, syndrome differentiation and accurate. 2, to grasp the meridian line followed Qi and blood
circulation. 3, relate about “bring about the desired sensation”. 4, illustrate relying on acupuncture point
23
and so on. At the same time, given an example as acupuncture needle, moxibustion, cupping, scraping,
etc in clinical application can direct the research.
A-032
Assessment of using the Tianxiangdan Capsule to Help Rehabilitation Therapy in
Coronary Artery Bypass Grafting
Li Jingru1, Wang Xiaofeng1*
1 Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should
be addressed to wxf87112008@163.com
Objective: To evaluate the clinical efficiency of using the Tian Xiang Dan Capsule to help rehabilitation
therapy in Coronary Artery Bypass Grafting (CABG). Method: 77 Patients of CABG randomly were
divided into treatment group (n=37) and control group (n=40). Control group were given regular treatment,
while treatment group were added the Tian Xiang Dan Jiao Nang with regular treatment, one month was a
period of treatment and three periods of treatments were observed the effect. Results: After a 3-month
treatment. Total effective rate in the treatment group was 78.38%.In the control group was 55.00% (P
<0.05); evidently effective rate in the control group was 2.5%, in the treatment group were 10.81%, two
groups were different significantly (P <0.05). Scoring of symptoms was decreased. ( P<0.05), The scores
in terms of physical functioning、
role-physical、
bodily pain、
general health，
vitality、
social functioning、
reported
health transition of patients in the experimental group according to SF-36 scale were significantly higher
than those in the control group (P<0.05 ). The scores in terms of physical limitation、
anginal
stability、
anginal frequency、
treatment satisfaction and Disease perception of patients in the experimental
group according to SAQ scale were significantly higher than those in the control group (P<0.05 ). In terms
of 6 min walking test was evidently effective rate in the treatment group than that in the control group
(P<0.05). The function of heart LVEF before and after the test, two groups were effective (P<0.05), but
there were not statistical different between two groups (P>0.05). Conclusions: (1) the Tian Xiang Dan
Jiao Nang can improve the relation symptom. (2) Tian Xiang Dan Jiao Nang can improve function of
LEVF、
the quality of life and the actual living ability of CABG patients.
A-033
Clinical study on Differential Treatment of Traditional Chinese Medicine in the treatment
of 210 Cases of Sub-health State in Urumqi
Liu Tao1, Hu Xiaoling1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should
be addressed to xjhuxl@yahoo.com.cn
Objective: To observe the Chinese medical type of syndrome characteristic and the clinical efficacy of
differential treatment of TCM in treatment of 210 cases of sub-health state, we will get some references
from the study. Methods: Using Task Force developed the questionnaire to screen the sub-health state
out of people, according to "Clinical Guidelines of Chinese Medicine on sub-health" dialectic, and to
identify syndromes, the study of evidence-based rules. Using different treatment of traditional Chinese
Medicine, observe the people for intervention treatment. The observation and assessment of its clinical
24
efficacy were observed by Symptom Rating Scale. Results: (1)210 Cases of Sub-health State with
syndrome of stagnation of liver qi the distribution of the majority (38.57%), followed by syndrome of qi
deficiency (31.43%), syndrome of yin deficiency of liver and kidney (20.48%), syndrome of phlegm-heat
attacking internally(9.52%); the card-type distribution in terms of gender difference was statistically
significant (P<0.05); various syndromes in all age groups the difference was statistically
significant(P<0.05).(2) there were obvious statistical differences between the collected scores of the19
main body symptom evaluated before the treatment and that after treatment(P<0.05). Conclusion:
(1)210 cases of sub-health state in Urumqi city, syndrome of stagnation of liver qi in order to permit the
most prevalent, many syndrome of stagnation of liver qi 、
syndrome of qi deficiency and syndrome of yin
deficiency of liver and kidney people are female, many syndrome of phlegm-heat attacking internally
people are male; Many syndrome of stagnation of liver qi people in 18～
39 age, syndrome of qi deficiency
people are older than 40 age. (2)In treatment of sub-health state differential treatment of traditional
Chinese Medicine effectively improves clinical syndrome.
A-034
Coronary Heart Disease merge 2-hour postprandial glucose patient of Traditional
Syndrome Differentiation and ZeXieDanMingYin intervention research
Zeng Lin1, An Dongqing1*, Li Kaili1
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should
be addressed to andongqing3@gmail.com
Objective: Explore cardiovascular hospitalized patients with abnormal condition of sugar metabolism,
Coronary Heart Disease merger 2-hour postprandial glucose patient of Traditional Syndrome rule,
observe CEC in Coronary Heart Diseas with diabetes mellitus and coronary merger 2-hour postprandial
glucose abnormalities in patients with level changes and discrepancy and oral ZeXieDanMingYin and
rational lifestyle intervention six weeks after observe its blood glucose, lipid profile and circulatory
endothelial cell parameters such changes.Methods:Select January 2010 to September 2010 period , in
Xinjiang Medical University affiliated hospital of traditional hospitalized patients diagnosed Coronary
Heart Disease、
Coronary Heart Disease with diabetes and Coronary Heart Disease merge 2-hour
postprandial glucose patients, observe CEC quantity change, Coronary Heart Disease merge 2-hour
postprandial glucose patients, according to Traditional Syndrome 6 dialectical divided into
XunXueYuZu, QiZhiXinXiong, TanZhuoBiZu, QiYinLiangXu, XinShenYinXu, XinShenYangXu, analyzing
its characteristics. The control group were randomly divided into two groups, with western medicine
foundation treatment, including regular enlarge crowns, lipid, step-down treatment such as, but not taking
oral, The treatment group based in western medicine treatment combined with alisma orientalis Dan Ming
drinks and rational lifestyle intervention six weeks observation blood sugar, blood fat and circulatory
endothelial cell parameters such changes. Results：
1、
2-hour postprandial glucose rate in Coronary
inpatients 62% 2、
Traditional syndrome differentiations with TanZhuoBiZu type at most, followed by most
XinXueYuZu type, 3、
When 2-hour postprandial glucose the circulatory endothelial cell significantly higher
than CHD group.4、
Add ZeXieDanMingYin and rational lifestyle intervention six weeks after can
significantly reduce TC 、
TG、
LDL – C、
PBG (P < 0.05)，
HDL-C、
LDL-C without opparent change (P > 0.05),
a clinical medicine effect is better (P < 0.05).Conclusion: China coronary inpatients in the vast majority of
merger sugar metabolic abnormalities, and need through OGTT experiments accurately find these
25
merger with abnormal glucose metabolism, always detection FPG diagnosis most sugar metabolic
abnormalities. Through the Traditional Syndrome differentiation coronary heart disease merger 2-hour
postprandial glucose in the process, we found that the most common TanZhuoBiZu, so we think in clinical
medicine treatment on the basis of add with HuaTanTongLuo drug of traditional Chinese medicine to
prevent coronary heart disease merger 2-hour postprandial glucose, reduce the morbidity and mortality in
patients with, raise its quality of life to have the extremely vital significance.
A-035
Experimental Study in Effects of Xu-zao-geng-ping Powder on the Histomorphology
Change of Arteries and MMP-9, TIMP-1 in Climacteric Hypertension Rats
Li Hongxia1, Lin Xue1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should
be addressed to linxueys@163.com
Objective: Through study of the effects of Xu-zao-geng-ping Powder in climacteric hypertension rats on
histomorphology change of vascular and MMP-9，
TIMP-1 research to explore reverses vascular
remodeling and possible mechanism in Climacteric hypertension rats. Methods: Surgical remove ovaries
of SHR rats to establish climacteric hypertension model and evaluate it. SHR rats were randomly divided
into climacteric hypertension traditional Chinese medicine, climacteric hypertension western medicine,
climacteric hypertension, fake operation and SHR groups. Set Wistar group as a normal control group.
Drench Xu-zao-geng-ping powder to climacteric hypertension traditional Chinese medicine group and
Valsartan capsule to climacteric hypertension western medicine. The other groups were drenched saline
solution. Observation of morphological changes in vascular tissues of rats in each groups, measuring MT,
LD, MT/LD, monitoring changes of blood pressure in groups of rats and comparing MMP-9, TIMP-1 level
in serum of different groups. Results: 1. Vaginal smear of model rats showed no renew of estrous cycle
change. Rat serum E2, T, P levels were significantly reduced(P<0.05); FSH and LH level were
significantly increased (P<0.05). 2. Compared with the climacteric hypertension group, fake operation
group and SHR group, blood pressure of climacteric hypertension traditional Chinese medicine and
climacteric hypertension western medicine group in rats dropped significantly before and after drenching
drug. 3. Morphological changes was significant in vascular tissues of climacteric hypertension rats. Their
LD reduce than other groups, MT, MT/LD significantly increased (P<0.05). LD of fake operation, SHR
group decreased than climacteric hypertension traditional Chinese medicine, climacteric hypertension
western medicine and Wistar group, but MT, MT/LD of fake operation increased (P<0.05). LD of
climacteric hypertension traditional Chinese medicine and climacteric hypertension western medicine
groups decreased than Wistar group, but their MT, MT/LD increased (P<0.05). 4. The serum levels of
MMP-9, TIMP-1 of climacteric hypertension group significantly increased than the other groups (P<0.05).
Compared with the climacteric hypertension, fake operation and SHR groups, MMP-9 levels in serum of
climacteric hypertension traditional Chinese medicine and climacteric hypertension western medicine
groups decreased, but TIMP-1 levels increased (P<0.05). Conclusion: 1. Established climacteric
hypertension rat model by removal of ovaries in SHR rats. 2. Vascular tissue pathological changes can be
increased in climacteric hypertension rats. 3. MMP-9 levels of serum increased to stimulate TIMP-1 level
of serum compensatory increased. Disorder of MMP-9 and TIMP-1 broke the dynamic balance result the
vascular remodeling. 4. Findings of this study indicate that the Xu-zao-geng-ping Powder can reduce
26
blood pressure in climacteric hypertension rats significantly and reduce vascular tissue damage caused
by reducing MMP-9 and increasing TIMP-1 in serum to restore ECM control balance. It probably is the
effective mechanism for preventing and reversing vascular remodeling in climacteric hypertension rats.
A-036
Experimental study on Inflammatory factors in serum of rats with Rheumatoid Arthritis
treated by PengCao Removing Bi-syndrome Decoction
Tian Xinwei1, Liu Chunjing1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China
Objective: To observe the mechanism of PengCao Removing Bi-syndrome Decoction(PCRBD) on
Rheumatoid Arthritis(RA) and the level of Inflammatory factors IL-1β、
TNF-α and PGE2 in serum of rats
with Rheumatoid Arthritis treated by the decoction. Methods: Randomed out 10 rats as blank group，
the
remaining 50 rats for replication collagen inductive arthritis (CIA) model，
divided them into Chinese
high，
medium，
low group，
tripterygium glycosides(TG) group and model group，
keeped the TG group as the
positive control group, observed 6 weeks，
evaluated the inhibition of PengCao Removing Bi-syndrome
Decoction on the joint swelling of the double back foot ，
the joint pain index of the double back foot，
the
weight gaining and the IL-1β、
TNF-α and PGE2 in serum. Result: Compared with model group，
the joint
swelling of the double back foot(P<0.01，
P<0.05)，
and the joint pain index of the double back foot
(P<0.01)of the rats in the treatment groups are significantly restrained；
and the rate of the weight gaining
improved significantly treated by the Chinese medium and high dose group (P<0.01，
P<0.05)；
the level of
IL-1β、
TNF-α and PGE2 in serum of rats are much better than the model group(P<0.01)；
the inhibitory
effect of Chinese medium dose group on the level of IL-1β、
TNF-α is better than the TG
group(P<0.05),the high dose group reduced the level of PGE2 better than the others. Conclusion: The
usage of PCRBD could restrain the joint swelling of the right back foot and the joint pain index of the
double back foot of the rats significantly. The mechanism of relieving the symptoms of rats with RA maybe
related to reducing the level of proinflammatory cytokines IL-1β，TNF-α and PGE2 in serum，
and the
decoction is a pure Chinese herbal compound worthy of further research and development.
A-037
A Study on Influence of Xu Zao Geng Ping granule on Blood Pressure Variability of the
Rats with Menopause Hypertension
Gan Qinglei1, Lin Xue1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should
be addressed to linxueys@163.com
Objective: Through study of the effects of Xu-zao-geng-ping-powder in menopause hypertension rats on
histomorphology change of BP, BPV and CA, NPY, NT, E2, FSH, LH research to explore reverses reduce
the BP and BPV possible mechanism in menopause hypertension rats. Methods: Established
menopause hypertension rats model by operating, model evaluate by the vaginal smear and serum sex
hormone levels in rats and randomly divided them into menopause hypertension TCM medicines,
menopause hypertension western medicine, menopause hypertension control group, fake surgery group,
27
SHR group and Wistaris a normal control group. Respectively to intragastric administration
xu-zao-geng-ping-granule to menopause hypertension TCM medicines group, valsartan capsule to
menopause hypertension western medicine group and saline solution to the others groups. Instrument for
non-invasive measurement of blood pressure(BP) in animals, at weekly measurements twice all of the
arterial blood pressure of rats to compare different groups in arterial BP for observing the changes in BP
of rats. Comparative observation changes of BP, BPV and CA, NPY, NT, E2, FSH, LH in different groups.
Results: 1.By results of the vaginal smear and serum sex hormone levels in menopause hypertension
model for evaluating success: compared with the fake surgery group and SHR group, model rats after
operation, menopause hypertension rats serum E2, T, P levels significantly reduced(P<0.05); menopause
hypertension rats serum FSH, LH levels significantly increased(P<0.05). 2.BP: TCM medicines group and
western medicine group’s BP is significantly declined(P<0.05). 3.BPV: compared with the menopause
hypertension control group, TCM medicines group and western medicine group’s BPV is significantly
declined(P<0.05). 4. Neurotransmitter indices: compared with the menopause hypertension control
group, TCM medicines group and western medicine group’s BPV is significantly increased(P<0.05). 5.
Endocrine indices: compared with the menopause hypertension control group, TCM medicines group’s
E2 is declined(P<0.05); TCM medicines group’s FSH, LH is increased(P<0.05). Conclusion: 1. Through
the removal of essential hypertension in rat ovaries, menopause hypertension rats model can be
successfully established. 2. The xu-zao-geng-ping-granule can reduce the climacteric hypertension rats’
BP and BPV. 3. The xu-zao-geng-ping-granule can regulate the neurotransmitter and endocrine indices
of the climacteric hypertension rats which improve the menopause hypertension rats’ internal
environment.
A-038
The characteristics of the syndrome TCM distribution of femoral head necrosis in Urumqi
region
Jin Wuyong1, Meng Qingcai1*
1Affilated Traditional
Chinese Medicine Hospital, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; *Correspondence
should be addressed to mengqincai@medmail.com
Objective: To explore the characteristics of the syndrome TCM distribution of femoral head necrosis in
Urumqi region and offer a reliable basis on therapy for femoral head necrosis in principles, methods,
prescriptions, drugs. Method: We collected 358 patients out of and in hospitalization who meet the
diagnosis standard of femoral head necrosis from our co-operation hospitals in Urumqi city randomly and
collected their information using the survey questionnaire of femoral head necrosis. The data were
2
analyzed by univariate frequency distribution, x test. Results: (1) blood stasis type, liver and kidney
deficiency type frequency in 28% to 64%, and Blockage of the meridians type and blood stasis blocking
collaterals type and frequency of each of 4%, blood stasis and kidney deficiency Between the imaginary
frequency of symptoms very significant difference (p<0.05); (2)Blockage of the meridians type and
frequency of phlegm and blood stasis blocking collaterals did not differ between; different age and gender
was significant difference between (p<0.05); (3) Different TCM syndrome distribution and gender, age
comparison, there was a significant difference was significant (p<0.05); (4) Comparison of different
syndromes, in addition to ethnic difference was not statistically significant, the remaining duration of
28
disease, incentive distribution, occupation, respectively, the difference was significant (p<0.05).
Conclusion: The TCM syndrome distribution of femoral head necrosis in Urumqi region is ：
The general
syndrome is qizhixueyu syndrome，
the next is ganshenkuixu syndrome，
jingluobizu and tanshizuluo
syndrome are rare.
A-039
The Correlation of Uygur and Han Nationality of TCM Syndrome Type of Premature
Coronary Heart Disease with Blood Lipids and Inflammatory Factor
Zhai Xueqin1, Wang Xiaofeng1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should
be addressed to wxf87112008@163.com
Objective: To investigate the correlation of Uygur and Han nationality of TCM syndrome type of
premature coronary heart disease with blood lipids and inflam-matory factor. Methods: Recruited were
152 Uygur and 156 han patients with PC-HD first confirmed by coronary angiography at the Heart Center
of First Affiliate-d Hospital of Xinjiang Medical University and Traditional Chinese Medicine of Xinjiang
Medical University. Another 160 volunteers were recruited internal medicine. T-CM syndromes were
typed physicians or those having higher professional titles at the cadiac internal medicine. The contents of
triglyceride (TG) and total cholester-ol (TC)were detected using oxidase method, the high-density
lipoprotein cholesterol(HDL-C) level was detected using chemical modification encymic method, the low
density lipoprotein cholesterol(LDL-C)level was detected using selective dissolution. The fibrinogen (FiB)
level was detected using Clauss congeal principle. The high-sensititive reactive protein (hs-CRP) level
was detected using Scattering turbidity method. The interleukin-6 level was detected by ELISA. Results:
(1) The distribution of TCM syndrome of Uighur PCHD as follows: Huizhuotanzu type(32.24%)> Qixuxueyu type (31.58%) >hanningxinmai type (18.42%) >qiyinliangxu type (10.53%)>yan-gqixushuai type
(7.24%); The han distribution as follows:Qixuexueyu type(35.90%)>Huizhuotanzu type(25.00%)>Hanningxinmai type(22.44%)>Qiyinliangxu type(12.18%)>yangqixushuai type (4.49%). (2)two nationals of
syndrome types exist fat metaboli-sm disorder obviously with PCHD patients, showing a significantly
higher level of TG, HDL-C level of reduced significantly, and LDL-C level rises, the statistics w-as
significantly difference (P<0.01, P<0.05); And han nationality with card model more, Huizhuotanzu type of
TG was higher significantly, HDL-C was fall, the stati-stical was difference significantly than han (P<0.01,
P<0.05), Hanningxinmai type of TG was higher significantly too, Qixuxueyu and Qiyinliangxu was higher
of T-G、
LDL-C significantly (P<0.01, P<0.05). (3) Two nationals of syndrome types exi-st inflammation
obviously with PCHD patients, the content of Fib、
hs-CRP and IL-6 were rise. The content of FiB、
hs-CRP
and IL-6 were increased in Uighur of P-CHD patients (P<0.01), Hanningxinmai of IL-6 and hs-CRP were
rising (P<0.01), Qixuxueyu of IL-6 was rising too; The content of hs-CRP、
FiB、
and IL-6 were increased in
Han of PCHD patients of Huizhuotanzu and Hanningxinmai(P<0.01), Qi-xuxueyu of IL-6 and FiB was
rising too(P<0.01). Two national of Hanningxinmai and Qixuxueyu were difference of FiB (P<0.05). (4)To
analysis the correlation of TG、
TC、
HDL-C、
LDL-C、
FIB、
hs-CRP and IL-6 of TCM syndrome type by
spe-arman rank. Uygur of PCHD patients of Huizhuotanzu of TG、
FiB、
IL-6 are rela-ted positively，
related
with HDL-C negatively, relevant coefficients: r=0.469, r=0.464, r=0.999，
r=-0.382. Qixuxueyu was related
positively with FiB, correlation coefficient：
r = 0.720, the differences are statistically significant (P<0.01,
P<0.05). Ha-n of PCHD patients of Huizhuotanzu of TG、
FiB、
IL-6 are related positively two，
related with
29
HDL-C negatively，
relevant coefficients: r=0.445, r=0.372, r=0.999，
r=-0.370. Qixuexuyu was related with
TG and FiB positively，
relevant coefficients we-re r=0.471, r=0.459, the differences are statistically
significant (P<0.01, P<0.05). Conclusion: The correlation of Uighur and Han nationality of TCM
syndrome type of premature coronary heart disease with blood lipids and inflammatory factor. Uygur,
which can provide the basis and the guide for the research for PCHD TCM syndrome type.
A-040
The Correlation of Uygur and Han Nationality of TCM Syndrome Type of Premature
Coronary Heart Disease with Molecular Markers of Prethrombotic State
Liu Hao1, Wang Xiaofeng1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should
be addressed to wxf87112008@163.com
Objective: To investigate the correlation of Uygur and Han nationality of TCM syndrome type of
premature coronary heart disease with the markers of prothrombotic state. Method: 626 eases
underwent CAG in Xinjiang Medical University, and were divided into PCAD 340 patients(154 cases Han
Uygur,186 cases of late onset CHD 286 patients(Uygur)in 106 cases,180 cases of Han)in two groups,
patients after hospital admission records for patients with traditional risk factors, by hospital heart internal
medicine attending physicians and above the syndrome differentiation of Traditional Chinese Medicine
typing, all the patients in the second days after admission to the morning fasting venous blood 2ml mining,
separation of serum. Fibrinogen(F g/FIB) using automated latex enhanced immunoturbidimetric method,
tissue plasminogen activator(T-PA), plasminogen activator inhibitor(PAI-1),using ELISA method for the
determination of-two; D dimer(DD), P-selectin(P-Sel)using the ELISA double antibody sandwich assay
test check. Results: (1)The Uygur PCAD patients of age, positive family history and Han Nationality early
onset group with a significant difference, age, smoking, drinking,overweight and Uighur late-onset group
CHD had statistical significance(P<0.01), Han PCAD patients in age, smoking, drinking, dyslipidemia,
hypertension, positive family history and Han late-onset group CHD had statistical significance(P< 0.05,P
<0.01).(2)the Uygur PCAD Qi Deficiency and blood stasis, turbid sputum an-d other syndromes and Han
PCAD were statistically significant(P<0.01),PCAD abusive cloud Uygur phlegm and other syndromes and
Uygur late onset group with ca-rd type comparison there is statistically significant(P<0.01).(3)To
compared Uygur And Han Nationality Of PCAD patients of PAI-1 Fg and DD with the same Nationality
groups are statistically significant，
the PAI-1 of turbid sputum syndrome, P-Sel, Fg, DD of Qi yin phlegm
blocking with the same Nationality syndrome groups are statistically significant; Uygur patients with
PCAD turbid sputum syndrome and P-Sel, T-PA,PAI-1,Fg and positively related, and DD is negative
correlation, turbid sputum syndrome and P-Sel, PAI-1, was positively related to Fg, T-PA is negative
correlation,Qi deficiency and blood stasis was positively correlated with DD. Conclusion: smoking,
drinking, overweight, dyslipidemia, hypertension, diabetes, in the Xinjiang region of the CHD patients are
accounted for a considerable proportion of the Uighur patients with PCAD, younger age, positive family
history of Han nationality patients with PCAD significantly increased(44.1%); turbid sputum, Qi deficiency
and blood stasis is the four group is the main type of Chinese medicine, and Uygur PCAD dirty chaotic
phlegm was significantly higher than that of Han nationality early-onset group; Uygur and Han nationality
patients with PCAD syndrome type of Traditional Chinese Medicine and the markers of Prethrombotic
state there is correlation, can serve as a guide for PCAD syndrome type of traditional Chinese Medicine to
30
provide basis for the study of the micro substance.
A-041
The Experimental Study of Qihong Decoction on the effects in rat model with
isoproterenol induced chronic heart failure
Wang Min1, Wang Xiaofeng1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should
be addressed to wxf87112008@163.com
Objective: To study the treatment of Qihong Decoction in rat model with isoproterenol induced chronic
heart failure and to observe the effects on brain natriuretic peptide (BNP) and matrix metalloproteinase
-9 (MMP-9) and to explore the mechanism of its prevention and treatment of heart failure. Methods: The
method of subcutaneous injection of isoproterenol copy cardiac failure model, determination of cardiac
function after 6 weeks that model successful, will become the model rats were randomly divided into
model group, low dose of Qihong decoction, Qi Hong granule middle dose group high dose of Qihong
decoction and digoxin group, were administered again after 4 weeks, cardiac function measured using
the enzyme immunoassay of serum BNP, MMP-9 levels, and take heart tissue that re-recording,
calculating the heart weight index of treatment, morphological features of cardiac pathology. Results:
①
compared with blank control group, the model group declined in cardiac function ,LVEDD、
LVESD
increased and EF、
FS decreased . Every treatment groups significantly improved cardiac function after 4
weeks, LVEDD、LVESD decreased and FS、EF increased.②
compared with blank control group，
heart
weight index was ignificantly increased among model group, Qi Hong low dose group and digoxin group,
while Qi Hong granule medium- dose and high- dose were no significant difference (P> 0.05); Compared
with model group, the treatment groups of heart weight and heart weight index decreased, there was
significant statistical difference (P <0.01). ③
Compared with blank control group, the content of BNP,
MMP-9 increased significantly in model group (P <0.01); compared with model group, the content of BNP,
MMP-9 decreased in every treatment group (P <0.01), Qi Hong granule which high, medium dose group
were obviously better than that of digoxin group (P <0.01), Qi Hong granule low-dose compared with
digoxin group no significant difference (P> 0.05).Conclusion: Qihong decoction can significantly alleviate
symptoms, improve heart function, regulating cardiac endocrine function in the heart and reverse
ventricular remodeling.
A-042
The Influence of Oubo Rhinitis Granule to Allergic Rhinitis Rats Th2 Cells and Its
Factors、
IgE and Mast Cells
Zhang Xinri1, Li Yanhua1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should
be addressed to liyanhua1953@126.com
Objective: The main objective of this essay is to study the impact of oubo rhinitis electuary for the Th2
serum cells of the anaphylactic rhinitis experimental rats and its other factors cells (IL-4, IL-5); to observe
the change of the nasal mucosa mast cells of the anaphylactic rhinitis experimental rats; to discuss the
31
foundation and therapeutic effect of the model of the anaphylactic rhinitis experimental rats. Methods:
There are 60 healthy adult wistar rats, which can be divided into same numbers of males and females.
These rats will be made for rat models with egg albumin sensitization. Then, the successfully models will
be going to random allocation. Next, after feeding them medicine for 28 days, it can draw blood from
carotid artery and separate their serum. Furthermore, the ELISA methods should be used to detect the
amount of total IgE, IL-4 and IL-5. Also the flow cytometry (FCM) should be used to inspect Th2 cells
contents of peripheral blood cells. Lastly, with 1% armor aniline blue staining watches the changes of the
experiment rats’ nasal mucosa mast cells. Results: 1) Compared with the normal groups, the serum level
of the model rats striking rise about IL-4, IL-5 and IgE (P<0.01), but the serum level of the therapeutic rats
bring down about IL-4, IL-5 and IgE (P<0.05). Also, the difference is remarkable between therapeutic rats
and the masculine rats (P<0.05). 2) Compared with the normal group, the Th2 cells of the model rats clear
grow (P<0.05); compared with the model group, the Th2 cells of the oubo rhinitis electuary small dose
group have no obvious difference (P>0.05); but the Th2 cells of the oubo rhinitis electuary medium and
high dose group evident decrease (P<0.05); at the same time, the Th2 cells of XinQin group sharp down
(P<0.05), the Th2 cells of the oubo rhinitis electuary medium and high dose group have no distinct change
when we compared with the XinQin group(P>0.05). 3) The Pathological pictures show the Mast cell in
nasal mucosa of model rats are increase, each give medicine rats are reduce, the average number of high
dose group’s Mast cell in nasal mucosa is minimum. Conclusion: In order to explore the antiallergic
effect and to alleviate the allergic rhinitis symptoms, the oubo rhinitis electuary may reduce the serum of
the experimental rats of the amount of IL-4, IL-5, total IgE and Th2 content and the nasal mucous
membrane of the number of mast cells.
A-43
The pathological effect of PengCao Removing Bi-syndrome decoction on synovial
inflamation and bone erosion of rats with Collagen-Induced Arthritis
Chen Chang1, Liu Chunjing1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;
To study the mechanism of PengCao Removing Bi-syndrome Decoction on Rheumatoid Arthritis(RA)
from the pathological and immunohistochemical perspective by observing Histopathology affect on
synovial tissue and the regulation mechanism of bone erosion of rats with collagen-induced arthritis(CIA)
treated by the decoction. Clean level Wistar rats，
60，
male and female evenly, weigtht (110±20)g，
Random
out 10 rats as blank group，
the remaining 50 rats for replication collagen inductive arthritis (CIA) model, 7
days after causing inflammation we began to observe and record systemic articular damage degree,
every 7 days record 1 times，
the rats with arthritis index (AI) > 5 points are made mould success. Then we
divided them into Chinese high，
medium，
low group，
tripterygium glycosides (TG) group and model group，
observe 6 weeks，evaluate the arthritis index(AI) , the pathological changes of synovial tissue and the
Osteoprotegerin (OPG)、
Receptor Activator of NF-κB Ligand (RANKL) expressions in cartilage of rats.
Compared with model group，the AI of Chinese group and Tripterygium group was significantly
lower（
P<0.05，
P<0.01）
,pathological changes in synovial tissue scores were significantly
different,（
P<0.05，
P<0.01）
,through pathological morphology observation， PengCao Removing
Bi-syndrome Decoction of high，
medium and dose group and TG group，
synovial cell hyperplasia degree
is lower than model group significantly，
inflammatory cells infiltration and synovial vasculitis performance
32
were significantly reduced. PengCao Removing Bi-syndrome Decoction could reduce the AI of rats，
and
inhibit synovial cell proliferation ，
fibrous tissue hyperplasia，
inflammatory cell infiltration and macrophage
infiltration，
which is a pure compound preparations worth of a further development and research. The RA
regulatory mechanisms of Articular bone erosion may be related to inhibition of RANKL expression in
articular cartilage of rat with CIA and of no obvious effect on the expression of OPG.
A-044
Treatment of QHD Tongluo Experimental Study of hyperuricemia
Wang Danhui1, Yushanjiang Aikemu1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China
Objective: The observation dispels wet removing extravasted blood to pass winds Fang Fills the stomach
to the big mouse blood uric acid, the kidney function, the empty stomach blood sugar, the empty stomach
insulin influence, discusses dispels wet removing extravasted blood to pass winds the side to treat the
hyperuricaemia the action mechanism. Method: Establishment hyperuricaemia big mouse model: The
SD big mouse take the adenine, the ethylamine butylacohol as makes the mold medicinal preparation,
establishes the hyperuricaemia animal model. Sets up the normal group，
the model group，
the western
medicine group，
the Chinese native medicine low monitoring team，
the high monitoring team. Feeds 3
weeks to observe separately tests 7 day, tests 21 day, determines the big mouse's blood uric acid，
the
urea nitrogen, the myo- bitter wine，
the empty stomach blood sugar，
the empty stomach insulin; Takes the
kidney left side in 21st days, observes the kidney pathology change. Results: Dispels wet removing
extravasted blood to pass winds the side to have the function which the suppression model big mouse
blood uric acid, the myo-bitter wine, the urea nitrogen, the empty stomach blood sugar, the empty
stomach insulin advance. Conclusion: Dispels wet removing extravasted blood to pass winds the side to
have reduces the hyperuricaemia model big mouse blood uric acid the function.
A-045
The Influence of Xihong Kang Fang Using to the diabetes nephrosis patient phase III Hcy
CysC and UAER
Feng Chengcheng1, Yushangjiang Aikemu1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China
Objective: To observe the clinical effect of “xihong kang prescription” used to diabetic nephropathy
(phase III) patients with homocysteine (Hcy), Cystatin C (CysC), urinary albumin excretion rate (UAER).
And to probe the clinical effect of xihong kang prescription clinical symptoms of the diabetic nephropathy
(phase III) patients. Methods: 62 subjects with diabetic nephropathy phase III patients were randomly
divided into the treatment group (32 cases) and the control group (30 cases). Patients of the treatment
group took xihong kang prescription with modern medicine conventional therapy. Patients of the control
group only took the modem medicine only. Two months after the treatment, compare the total effective
rate of homocysteine(Hcy), CystatinC(CysC), urinary albumin excretion rate(UAER), fasting
blood-glucose (FBG), Two hours after a meal blood sugar(2hPG), Glycated hemoglobin(HbA1C), urea
nitrogen(BUN), Creatinine(Scr). As indexes are compared, observe the TCM syndrome change of early
33
diabetic nephropathy before and after the treatment. Results: The total efficiency of xihong kang
prescription in treatment of diabetic nephropathy(III)in the treatment group is 87.5%. After the treatment of
TCM syndromes in total, there were significant differences (P<0.05) in two groups. Compare Hcy, CysC,
UAER, FPG, 2hPG, HbA1c, statistically, dates of the treatment group is superior to that in the control
group (P<0.05 or P<0.01). After the treatment, compare BUN, Scr, statistically,there was no significant
difference in two groups (P>0.05). Conclusion: (1) Through this research we found that the xihong kang
prescription is an effective prescription in the treatment of diabetic nephropathy (phase III). The effect of
traditional Chinese medicine with western medicine therapy is better than pure western medicine
treatmentand the clinical symptoms obviousiy improved. (2) By probing Hcy CysC UAER we found that
Hcy CysC UAER is superior to Cr in evaluating the index of damaged kidney function and it can be used to
evaluate early renal damage of the convenience of the diabetic nephropathy accurate and reliable index.
(3) Through the observation of the treatment group and control group, compare Hcy CysC UAER before
and after treatment, we found that combined Traditional Chinese and Western Medicine treatment in early
diabetic nephropathy has more advantages than the pure western medicine therapy.
A-046
Clinical Analysis of 200 Cases of Curative Effect Factors on Treating Knee Osteoarthritis
with Small Needle Knife
Ma Lei1, Meng Qingcai1
1Affilated
Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，
Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China; *Correspondence should be addressed to mengqincai@medmail.com
Objective: Validation small needle knife's effect on knee osteoarthritis, for the more, to discuss the
different factors that is closely related with the result. Method: Choose 200 cases of knee osteoarthritis
patients， according to the standard who are only treated with small needle knife. After treatment, the
result would be observed four and eight weeks later. The factors influenced the effect of further line
Logistic regression analysis. Results: The material treated with small needle knife after four and eight
weeks, the total effective rate was 95.55% and 94.0% respectively, compared with before treatment, the
difference was statistically significant (P<0.05); The Logistic regression analysis: there were four factors
including disease course, weight, room number and deformed joint degree, prognosis for independent
risk factors, and it is the heavier weight, the longer duration, the more serious deformed joint degree, and
the more number of room, the worse effect; there were two factors X-ray classification and disease point
number for prognosis of the independent protection factor: the result in the short term is when the X-ray's
change more serious，treatment of patients with more disease points, the curative effect will be better.
Conclusion: Needle knife is a kind of safe and effective treatment. According to each patient's different
situation, make personalized treatment case, short-term curative effect is better, but the long-term
efficacy needs further clinical validation and observation.
A-047
The study of Clinical Curative Effect on Jing Yi An Shen Powder on 104 Cases of
Insomnia Treatment by Acupoint Application
34
Wei Qing1, Hu Xiaoling1
1
Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，
Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China; *Correspondence should be addressed to xjhuxl@yahoo.com.cn
Objective: To observe the clinic curative effect of Jing Yi An Shen Powder by acupoint application on
insomnia. Methods: In clinic study, 104 cases of insomnia were divided into three groups at random,
experimental group (n=38), diazepam group (n=34) and bran control group (n=32). The experimental
group and bran control group were treated with acupoint application method, while the diazepam group
using drugs.7 days for a course, two weeks later, we observed the effect on patients. Results: 1. Jing Yi
An Shen Powder improve the total effective rate of insomnia is 81.6%, while the diazepam group is 82.4%
and the bran control group is 34.4%. In the statistical analysis, the experimental group and diazepam
group compared with the bran control group (P< 0.01), difference was statistically significant compared;
the experimental group compared with diazepam group (P<0.01). there is not statistically significant.2.
After the treatment，
the experimental group、
diazepam group and the bran control group in Epworth
sleepiness total amount control comparing have statistical significance (P<0.01). 3. Jing Yi An Shen
Powder acupoint sticking apply to improve sleep quality rate of 68.4%, diazepam group to improve sleep
quality rate of 67.6%, bran control group to improve sleep quality rate of 27.3%.After treatment, the
comparison between groups, the experimental group、
diazepam group and the bran control group in Sleep
efficiency, sleep disorders, daytime dysfunction and total comparing have statistical significance
(P<0.05). 4. Jing Yi An Shen Powder acupoint sticking improve TCM syndrome effective rate is 86.8%,
while the diazepam group is 52.9%, and the bran control group is 37.5%. In the statistical analysis, the
experimental group compared to diazepam group and bran control group have statistical significance
(P<0.05). After treatment, the comparison between groups, the experimental group compared to
diazepam group and the bran control group in morning sleepy, palpitations yi jing, the mouth of the bitter
dry throat, TCM symptoms integral falls down have statistical significance (P<0.05). the experimental
group、
diazepam group compared to the bran control group in limbs were heavy, forgetfulness, sweat out
and total credit of TCM symptoms integral falls down have statistical significance (P<0.05).The
experimental group compare to the bran control in Improve inattention aspects TCM symptoms integral
falls down have statistical significance (P<0.05). Conclusion: Jing Yi An Shen Powder can effectively
extend the sleep time, improve sleep quality, and improving associated symptoms, thus improve the
clinical efficacy.
A-048
Clinical Curative Effects on Repeated Shallow Puncture with Filiform-fire Needle
on Herpes-zoster
AyijiamaliTuohuti1, Liu Hongxia1*
1Affilated
Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，
Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China; *Correspondence should be addressed to hongxialiu04@yahoo.com.cn
Objective: To observe the clinical curative effect of repeated shallow puncture with Filiform-fire needle on
Herpes zoster, evaluation repeated shallow puncture with Filiform-fire needle is better than ordinary
acupuncture praise treatments for the superiority of herpes zoster. Methods: 70 cases of herpes zoster
35
were selected by the patient in hospital order Numbers, by means of random Numbers table into two
groups. The treatment group for ordinary acupuncture, Repeated shallow puncture with Filiform-fire
needle joint foundation treatment method; The control group for the common foundation with acupuncture
therapy, and basic therapy for: Chinese medicine internal and (a tonic liver tonga diarrhea reduced),
Chinese medicine collapse stains therapy Cooperate with cutem therapy. Ordinary acupuncture choose
acupuncture point: local wai stab, ridge with side clip acupoint and Distal match point
(TaiChong,YangLingQuan, LiangQiu,HeGu, ZuSanLi, SanYinJiao), Repeated shallow puncture with
Filiform-fire needle is the third day of treatment, her lesions local use 0.25 x 0.25 mm of HaoZhen fast
evenly spread thorn, straight into the straight out, slightly into the skin namely stop, then use the proper
size cupping adsorption on treat place, leave cans of 5-10 minutes, the tank with disinfection after cotton
wipe local, according to the need to get 2-3 days and then a treatment. Treatment in observing herpes
number, erosion, erythema, edema and change; And in the third days before treatment, treatment and
after treatment for pain score, record the time, began to blister scabby time, all the scab off time, half the
time required for reducing pain, pain duration. Results: Finish the 70 patients with clinical curative effect
and the control group had no significant differences (P > 0.05), two groups before and after treatment
symptoms and signs giving a mark was not statistically significant (P > 0.05); The third day after treatment
and therapy, the treatment group was better than control pain relief degree; 2 the blister, start time of time,
all the scab off scabby time, reducing pain half the time required for constant pain, all have time
significance, and the treatment group than in control group. Conclusion: cooperate with Repeated
shallow puncture with Filiform-fire needle for Herpes zoster has good curative effect, relative to the
control group can quick pain relief, accelerate the scabby herpes, shorten the time constant pain,
enhance the curative effect, short treatment course, prevent the sequela of neuralgia, reduce cost, is safe
and effective.
A-049
Clinical Effect on DLK- A for Treatment and Prevention of Metabolic Syndrome (MS), on
Blood Sugar, Blood Pressure, Blood Lipids and Weight
Palidan Wushuer1, Nurmuhammat Amat2*, Duo Likun3
1 Neurology Department, Affiliated Traditional Chinese Medicine Hospital
Uighur Autonomous Region, PR China;
2Traditional
Xinjiang Uygur Autonomous Region, PR China
;3
，Xinjiang Medical University, Urumqi-830000, Xinjiang
Uighur Medicine Institutet, Xinjiang Medical University, Urumqi-830011,
Salamat Biotechnology Co., Ltd. Xinjiang 830011, China；*Correspondence
should be addressed to nur818@hotmail.com
Objective: By using medical laboratory technique, to evaluates the effectiveness of DLK in treating
metabolic syndrome. Figure out and verify the effectiveness of DLK's on metabolic syndrome. Methods:
Using double-blind contrast clinical test, Taking weigh index greater than 25 as a standard (BMI> 25), the
volunteers were divided into experimental group and control groups applying DLK and placebo
randomized, The main observation indexes were weight, FBG, fasting insulin level, blood pressure, blood
lipids, HBALC and FMN. Various indicators were measured before and 30 days after the experiment.
Compare the improvement of DLK and placebo on MS, by using medical laboratory technique to test the
weight, FBG, blood pressure, blood lipids before and after the treatment. Results: According to statistical
results, experimental group had significantly lower indicators than control groups in weight, FBG, blood
pressure, CHOL, triglyceride, LDL and fasting insulin level(P <0.05). HINS is a manifestation of insulin
36
resistance and insulin resistance is the main cause for MS. According to statistical results, and
experimental group had significantly lower indicator than control groups in fasting insulin level, indicating
DLK significantly improving insulin resistance consequently showing potential value of DLK in prevention
and treatment of MS. Conclusion: DLK had decreased blood sugar, and blood pressure, blood lipids
(including triglycerides and CHOL), especially weight. DLK, not only as a natural substance, is eligible to
be developed into a functional food or nutritional supplement. But also have high safety and effectiveness.
A-050
Clinical observations on chronic prostatitis of dampenss-heat down-flowing stasis with
qianlieshuang decoction
Sha Mo1, Ma Chao1
1Affilated
Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China
Objective: This thesis mainly discussed the research of Chronic Prostatitis caused by the inflammatory of
dampness-heat down-flowing through the treatment of Qianlieshuang Decoction by the way of observing
the curative effect and the influence of the observation index and meanwhile made a contrast treatment
with Qianlietongyu Capsule. Also with the purpose of exploring the therapeutic action of Qianlieshuang
Decoction at the treatment of Chronic Prostatitis, and providing clinical evide-nce for the Chinese Medical
Treatment. Method: With the randomized, controlled method, 80 Chronic Prostatitis patients that caused
by the inflammatory of dampness-heat down-flowing were selected, 40 in the treatment group
(Qianlieshuang Decoction) while 40 in the control group (Qianlietongyu Capsule).Observed the symptom
score of treatment of Chinese Medicine, NIH-CPSI score, prostate fluid routine examination of changes in
indicators before and after the treatment .Do the statistical analysis and draw the conclusion. Result:
Treatment group: clinical cure: 11 cases, significantly effective: 14 cases, effective:10 cases. the clinical
cure rate, the significantly effective rate, the effective rate and the total effective rates were
27.5%、
35.0%、
25.0%、
87.5%.Control group: clinical cure:5 cases, significantly effective:11
cases,effective:8 cases. the clinical cure rate, the significantly effective rate, the effective rate and the total
effective rates were12.5%、
27.5%、
20.0%、
60.0%. The treatment group is significantly better than the
control group at comparison of the symptom score of treatment of Chinese Medicine, NIH-CPSI score,
prostate fluid routine examination with the result of P<0.05. Conclusion: Qianlieshuang Decoction has
significant function on the treatment of Chronic Prostatitis caused by the inflammatory of dampness-heat
down-flowing, concentrated expressed in the improvement of symptoms and Laboratory tests index, and
has not found side effects yet. This kind of treatment Is a relatively safe, economical and effective
treatment methods.
A-051
37
Clinical Study on combination of Traditional Chinese Medicine and Acupuncture of
Promoting the Recovery of Gastrointestinal Function in the Treatment of Postoperative
Patients with damp-heat type
Guli Shaera1, Liu Zhiyan1
1
Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，
Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China
Objective: To observe the clinical efficacy of acupuncture combined with drug on recovery of
gastrointestinal function of damp-heat in the abdomen after surgery, and to explore effective methods of
acupuncture combined with drug to treat gastrointestinal disorders after damp-heat pattern abdominal
surgery. Methods: 65 patients of damp-heat pattern after abdominal surgery in accordance with the
principle of randomized controlled were divided into 34 cases of control group and 31 cases of treatment
group. The control group was given oral formula, twice a day, for 7 days totally; treatment group on this
basis, receive acupuncture treatment in the morning of the second day after surgery, once a day, for 7
days totally also, evaluate the efficacy of the two groups at 7 days after surgery. Compare the two groups
of patients with recovery of gastrointestinal function of time after surgery, the common clinical symptom
score changes, the efficacy results, the VAS pain score scale (VAS) and peripheral white blood cells
before and after treatment. Result: (1) treatment group, the first bowel sound and the first exhaust
defecation time than the control group was significantly shorter, the difference between the groups was
statistically significant (P<0.05). (2) The analgesic effect of the treatment group was better than the control
group, significant differences between the groups (P<0.05). (3) treatment group in improving anorexia,
abdominal distention, abdominal pain, defecation difficulties in clinical symptoms was better than the
control group, the difference between the groups was statistically significant (P <0.05). (4) Control group,
the total effective rate was 66.7% total effective rate of 80% was statistically significant (P <0.05). (5)
Treatment group after 5 days in peripheral blood neutrophils compared with control group decreased, the
treatment group after 5 days in peripheral blood lymphocytes than in the control group increased, the
difference between the groups was statistically significant (P≤0.05). Conclusion: Acupuncture
treatment can shorten the patients after discharge, bowel movement time; the oral combination of
traditional Chinese medicine also has analgesic and improves clinical symptoms and other effects, and
postoperative peripheral white blood cell changes in bidirectional positive regulatory role.
A-052
Decoction of Bushentongluo and its Decomposed in Repair of Articular Cartilage Defects
in Rabbits
Li Leijiang1, Lu Yong1
1
Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，
Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China; *Correspondence should be addressed to xjluyong@163.com
Objective: The treatment of the role of research Bushentongluo decoction and its decomposed decoction
on rabbit articular cartilage defects repair，
Clear the "decoction of Bushen", "decoction of Tongluo" and
"decoction of Bushentongluo” whichever is more effective in repairing articular cartilage defects
treatment. Methods: Take the drilling of 60 New Zealand white rabbits with left knee medial femoral
condyle center, diameter 3.5mm, depth of 3mm, up to the subchondral bone, resulting in full-thickness
38
articular cartilage injury. After surgery will be randomly divided into five groups (n=12). Group A:
Decoction of Bushentongluo to cooperate with the hyaluronic acid; group B: Decoction of Bushen to
cooperate with the hyaluronic acid; group C: Decoction of Tongluo to cooperate with the hyaluronic acid;
Group D: hyaluronic acid group; group E: model control group. After the modeling, the A, B, and C were
given Chinese medicine decoction 3mL / kg orally; A, B, C, D, four groups a week after hyaluronic acid gel
0.5ml line puncture of the left knee joint cavity, once a week for five weeks in a row; special treatment of
the E group did not. 8 and 12 weeks after surgery, respectively in each group were sacrificed 4, cut a left
knee medial femoral condyle observed defect in cartilage repair, parallel HE staining, Safranin O staining
and immunohistochemical staining, light microscopy the shape of the cartilage defect repair, the level of
expression of Collagen II histomorphometry score. Take 12 weeks after cartilage specimens by
transmission electron microscopy observation. Results: After four weeks in each group the cartilage
defect repair tissue is not obvious morphological different. 8 weeks after surgery in each group the
cartilage defect repair tissue morphology. After 12 weeks in group A cartilage defect repair tissue surface
is smooth, hyaline cartilage cells, immunohistochemical staining strongly positive. Group B and Group C
defects chondrocytes increase in the number of close to normal mature cells; the overall situation
compared with group A poor. Group D defect repair tissue is mostly fibrous tissue, a small amount of
cartilage cells. Rough group E defect repair tissue, and more fibrous tissue, A combination of two scoring
after 12 weeks A, B, C, three sets of scores were significant difference with Group D score, P<0.05; B;C,
the score of the two groups with Group A score significant difference, P<0.05; group B and group C scores
were the number was no significant difference, P> 0.05.Conclusion: The decoction of Bushentongluo,
the decoction of Bushen and the decoction of Tongluo have a significant therapeutic effect to cartilage
defects, the efficacy of Bushentongluo is slightly better than the decoction of Bushen and the decoction of
Tongluo, decoction of Bushen and decoction of Tongluo had no significant effective differences.
A-053
Effect of Jangzhuoshengqingtang on Pathology organization morphological、
plaques
area and Inflammatory Reaction on Atherosclerotic in Aorta of Apo-E-gene knockout
Mice
Wang Jipeng1, Xin Xiaoyi1
1
The First Affilated Hospital, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China
Objective: To use Apo-E gene knock out mice atherosclerosis modeling jiang zhuo sheng qing
tang of turbidity atherosclerosis inflammatory reaction mechanism, effect intensity evaluation.
Research jiang zhuo sheng qing tang of turbidity As the main function of the inflammatory
response targets, and regulation of the protection mechanism of the link. Methods: Thirty-five
Apo-E knockout mice were fed a high-fatdiet for 6 weeks. Five mice were chosen and stained
with HE until the atherosclerotic plaques formed. they were randomly divided into three groups:
model group(jiang zhuo sheng qing tang)，Chinese medical control group(bie jia ruan gan
pian)，medical control group(withsimvastain). Three groups were threaded with respective drug
in clinical recommended for another 6 weeks accompanied by feeding common diet. The
changes of IL-6、TNF-a in serum and plaques area in Atherosclerotic were detected. Results:
A11 the groups could decrease the level of serum IL-6、TNF-a，slow the atherosclerosis
development degree and reduced the plaques area，and the effects in jiang zhuo sheng qing
39
tang group and with simvastain group were better than that in bie jia ruan gan pian group；And
they could reduce the lipid as well as the lipid to collagen content in plaque. Conclusion: Jiang
zhuo shengqing tang has the turbidity atherosclerosis function, its mechanism may cut IL-6,
TNF-a expression of the relevant.
A-054
Experimental Study of Effect on Aplastic Anemia Model Rat’s Regulation Factors
IL–2、
TNF–a Treated with Tonifying Kidney and Replenishing Essence Method
Xie Rong1, Shan Lijuan1
1
Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，
Huanghe Road, Urumqi, Xinjiang Uighur
Autonomous Region, China
Objective: To observe the effect of Aplastic Anemia the model rat’s Regulation Factors’ level treated with
Tonifying Kidney and Replenishing Essence Method, investigate the mechanism of action. Methods:
Clean-class Wistar rats were divided into 4 groups，
namely blank group, model group, treatment group
(Tonifying Kidney and Replenishing Essence basic prescription, TKRE) and control group(Aplastie
Anemia Blood-engendring tablet) respectively. Rats were given the 5-FU plus Myleran to induce AA
model of rats. The blank group rats were fed normal foods, after judge AA model succeed, the treatment
group and control group rats were fed with TKRE prescription and Aplastie Anemia Blood-engendring
tablet for 30 days, After the experiment ended, to observe each group of rat’s general condition and
symptoms, test each rat’s circumference blood cytometry, the marrow pathology and the level of IL–2 and
TNF–a. Results: (1)Rat’s general condition and symptoms: Compared with model group, the treatment
group and control group rat’s spirit diet and motion were improved, quality of life raised; (2)Circumference
blood cytometry: Compared with model group, the numbers of blood cells in treatment group were
increased in varying degrees, the difference were statistically significant (P<0.05).Compared with control
group, the treatment group has significantly rise in white blood cell, the blood platelet and the net weaves
the red blood cell number, the difference were statistically significant(P<0.05). (3)Marrow pathology:
Compared with the model group, the treatment groups' marrow proliferation restored, the three System
cells proliferate, un-hematopoietic tissue and adipose Tissue reduce relatively, the hematopoietic
structure restored, the treatment group bring about a striking effect than control group. (4)Cell Factors IL-2
and TNF-α: IL-2 and TNF-α levels were significantly elevated in model group and treatment groups than
the blank group. Compared with model group,IL-2 and TNF-α levels were declined in treatment group, the
difference was statistically significant(P<0.05).Treatment group compared with control group, the IL-2 has
difference in the two groups (P<0.05), the TNF-α has no difference in the two groups (P>0.05).
Conclusions: According to the experiment show that the tonifying kidney and replenishing essence
method could significantly improve rat’s general condition and symptoms, promote rat’s circumference
blood cells, improve bone marrow hematopoietic function of aplastic anemia model of rat, Take into
account that the tonifying kidney and replenishing essence method can effectively improve abnormal
secreting of regulation factors, encouraged the bone marrow hematopoietic function’s recover, through
achieving the therapeutic purposes.
A-055
40
Literature Research on Prescription Rule of Oral Drugs for Osteoarthritis
Chu Litao 1, Lu Yong1*
1Affilated
Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi, Xinjiang Uighur
Autonomous Region, China; *Correspondence should be addressed to xjluyong@163.com
Objective: To explore the application range and prescription rule of the oral drugs of GuBi in Ancient and
modern literature in the different periods, And further analyze its pathogenesis and its therapeutic
treatment. For future clinical and scientific research, to provide document foundation and more explicit
medication guide. Methods: Investigate mainly the oral drugs of osteoarthritis literature material of
Traditional Chinese Medicine from the Qin and Han Dynasty to the modern times, and identify, reorganize
the original documents. There are 44 1topical prescription in this study, Each other medicine information
applications frequency analysis, variance analysis, cluster analysis, mathematical statistics method and
combining analysis induction, formula metrology research methods, party card corresponding methods
GuBi for use in formula of law of research and analysis . Results: Cumulative frequency of Chinese herb
reaches93. 69%, which are for restoratives Chinese Medicinal Herbs, Chinese Medicinal Herbs for
invigorating the blood and removing blood stasis, Antirheumatic Chinese Medicinal Herbs, diaphoretics or
exterior syndrome relieving Chinese Medicinal Herbs, warming the internal, Chinese Medicinal Herbs for
promoting diuresis and resolving dampness, heat clearing Chinese Medicinal Herbs, Chinese Medicinal
Herbs for calming the liver to stop endogenous wind, qi regulating drugs and antitussive and antiasthmatic
Chinese Medicinal Herbs are the main treatment drugs for osteoarthritis. They are the main drug
categories warm, neutral and cold and the most that accounted for 88. 77% in four nature. Sweet pungent
and bitter are the most that accounted for 93. 40% in the five flavors. Cumulative frequency of liver, heart,
kidney, spleen and lung is 91.14%, Through the system clustering formed 10 clustering prescriptions .
There are different in efficacy, four climates, the five kind flavors and pertaining meridian . Conclusion: 1.
The prescription rule of the oral drugs of GuBi are as follows: (1) Used in conjunction treatment of Xinkai
Kujiang and Ganwen Bingshi; (2) Adjusting viscera placidly to get rid of pathogens; (3) Fill in the diarrhea,
diarrhea in complement, (4) insect drugs significant effect. (5) The vines of the genus, pass through into
the network. 2. General use in gansu and Michael essien, taste bitter primarily, medicinal properties to
warm, neutral, cold is given priority to, be in by the liver, kidney, the heart sutra by the lung, spleen, by
primarily, focuses on the liver and kidney spleen. Drug class to make up for restoratives Chinese
Medicinal Herbs, Chinese Medicinal Herbs for invigorating the blood and removing blood stasis,
Antirheumatic Chinese Medicinal Herbs primarily. 3. Through the prescription that the osteoarthritis of
basic prescription type has five: Respectively for FengHanshiBi type, FengShiReBi type, TanShiNeiZu
type, GanShenKuiXu type. 4. Virtual, blood stasis, phlegm, Pathogenic is the main factor of the disease,
Total treatment on having to WenTong therapeutic mainly, and concurrently to remove stasis expectorant,
dispelling rheumatic and tong collaterals. 5. Through the analysisi of the 10 cluster prescriptions, special
drug on the following categories: Rectifying the drug Chenpi Banxia, Qianghuo Duhuo, Duhuo Xixin,
Rectifying and Centralizer drugs:Gouji Gusuibu, Chishao Baishao, Shengdi Mudanpi, Fuzi Ganjiang.
Centralizer drugs:Huangqi Baizhu, Tusizi Gouqizi, Fuzi Rougui, Danggui Shudi, Danggui Chuanxiong.
A-056
41
The Research on the Primary Immune Thrombocytopenia of Different Syndrome Types
and Characteristics of Platelet Parameters
Wang Yahong1, Bai Yusheng1
1
Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，
Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China
Objective: To discuss the relation of different Syndrome type and platelet parameters in Primary
immunity thrombocytopenia (ITP) and seek the reference for objective target of TCM (traditional Chinese
Medicine) syndrome type and curative effect. Methods: To make better observation table of clinical
cases, 126 patients with ITP were in accord with standard and the basic information, laboratory
examination indexes, clinical symptoms, tongues, and pulses in these patients were collected before
treatment. The tester judged syndrome type in ITP patients by unified standard，
collected platelets
parameters and analysed the relationship between TCM syndrome type and platelet parameters. Result:
The ages in Patients among the types in blood heat, yin deficient, and blood stasis impedance type
showed statistically significant differences (P<0.05); In the distribution of TCM syndrome types, the
largest number of types was failure of qi to check blood and yin deficiency, but the blood heat and
blood-stasis obstruction accounted for relatively small percentage of all types. The platelet count and
thrombocytocrit in different types among blood-stasis obstruction, blood heat, yin deficiency and failure of
qi to check blood were statistically significant differences (P<0.05);The mean platelet volumes in different
types among blood-stasis obstruction, blood heat, yin deficiency and failure of qi to check blood were
statistically significant differences (P<0.05);The platelet distribution widths in different types among
blood-stasis obstruction, blood heat, yin deficiency and failure of qi to check blood were statistically
significant differences (P<0.05);Conclusions: The syndrome type of TCM of ITP and platelet parameters
have significant difference and platelet parameters provide objective reference for TCM syndromes of
ITP; the correlation between TCM syndrome and platelet parameters predict different syndrome types of
TCM have a certain reference value towards the prognosis of efficacy
A-057
The research on Qufeng antispasmodic to asthma scattered (wind phlegm wheezing)
patients with pulmonary function, FeNO influence
Bai Wenmei1, Li Fengsen1*
1
Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，
Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China；
*Correspondence should be addressed to fengsen602@163.com
Objective: To observe the antispasmodic for chronic scattered bronchial asthma duration wind phlegm
wheezing patients with pulmonary function, breathe the influence of nitric oxide. Methods: 60 patients
with chronic duration with bronchial asthma wind phlegm wheezing patients were randomly divided into
the treatment group and control group of 30 cases of 30 cases, both groups to hormone inhaled
treatment, the treatment group and give oral qufeng antispasmodic scattered. 8 weeks of treatment.
Observe both groups of patients before and after treatment symptoms, signs, pulmonary function, breath
nitric oxide changes. Results: treatment group patients before and after treatment mean the FEV1 %
were 77.13%, 93.34%, and had more significant statistical difference (P<0.01), and the control group of
42
patients before and after treatment FEV1 % were 84.56%, 73.87%, had significant both statistical
differences (P < 0.05); The treatment group of patients before and after treatment mean FeNO were 63.04
ppb, 32.17 ppb, had more significant statistical difference (P<0.01), and the control group of patients
before and after treatment mean FeNO were 59.04 ppb, 55.02 ppb, had no significant statistical difference
(P > 0.05). Conclusion: qufeng antispasmodic scattered chronic duration can improve the bronchus
asthma wind phlegm wheezing of patients with lung function, can reduce the duration asthma patients
airway inflammation, bronchial hyperresponsiveness and breathe nitric oxide level.
A-058
Research on Catharsis and Moisturizes Theoretical Literature of Traditional Chinese
Medicine
Chen Lu1, Zhou Guang1
1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China
Objective: This issue through the collation of ancient and modern skin-related literature of Traditional
Chinese Medicine. Combined with the statistical analysis of the common skin disease related syndromes,
pathogenesis, legislation and prescription drug laws in the medical records of dynasties masters, To
comb and clarify the skin Catharsis and Moisturizes physiological basis ,functional performance and the
relationship among Ying -Wei ,zang fu and choroid; and its dysfunction pathogenesis characteristics,
Summed up the legislation and prescription medication law based on the theory of Catharsis and
Moisturizes, Provide the basis for the establishment of Chinese medicine dermatology disciplines
Catharsis and Moisturizes theoretical system. Method: Use of systematic literature research methods, to
collect and record theoretical basis of skin-related information from the Qin and Han Dynasty to the
modern times, and identify, reorganize the original documents. To establish the theoretical basis of
Chinese medicine skin database; cluster, stratified sampling to select the various periods representative
of common skin disease masters medical records,with prescription metrology and frequency analysis,
analysis of variance for statistical analysis of the literature information. Results: A total collection of skin
theoretical basis of literature information 1996,363cases of common skin disease medical records
information.The organizational structure of the Chinese medicine skin including striae, hairs, XuanFu, fur,
claws, coetaneous region, FuLuo, etc; the physiological basis and functional performance of the Chinese
medicine skin including excretion of sweat, the catharsis conditioning internal and external qi, to clear the
blood and body fluids, evacuation exogenous pathogens, adjust the cold and heat, stirs the body fluid to
moisten the skin; in these cases, skin catharsis and moisturizes dysfunction in syndromes and
pathogenesis of skin disease have universal and characteristic; Famous Medical Records legislation and
prescription drug based on the catharsis and moisturizes there was a marked convergence; pruritus,
desquamation, flush, swelling, ache, rough, hypertrophy, exudation, drying is the most common symptom
of skin disease, pathogenic factor lag skin, skin dry and heating is the most important partial
pathogenesis of skin disease; catharsis, heat clearing and pathogenic expelling; moisten is the common
rule for treat skin disease; clearing away heat and damp, clearing camp and cooling blood, dispelling wind
and heat is the main treatment methods of skin disease .Heat clearing Chinese Medicinal Herbs,
restoratives, diaphoretics or exterior syndrome relieving Chinese Medicinal Herbs, Chinese Medicinal
Herbs for promoting dieresis and resolving dampness，
Chinese Medicinal Herbs for invigorating the blood
and removing blood stasis, Chinese Medicinal Herbs for calming the liver to stop endogenous wind, qi
43
regulating drugs and Anti-rheumatic Chinese Medicinal Herbs are play a major role in the clinical
treatment. Cold､warm and neutral are the most in four nature; Bitter, sweet and pungent are the most in
the five flavors; meridian mainly in the liver、
heart、
lung、
spleen and stomach. Prescriptions are different in
the five kind flavors and pertaining meridian by drug administration in the ancient、
modern and periodicals.
Conclusion: Catharsis including skin permeate and opening and closing, and the physiological status
and functional performance of Moisturizes including skin moist and body fluid, blood compresses.
Catharsis and Moisturizes is specifically reflected in the form of the wei external function of skin, is also
the functional performance on the skin effect of Ying -Wei ,zang fu and choroid. Its dysfunction is not only
a skin disease main pathogenesis, but also the theoretical basis of the legislation and prescription
medication.
A-059
The influence of Spleen detoxification soup for murine spleen deficiency type psoriasis
ET-1, E-selectin and of PCNA
Guo Shanshan1, Liu Hongxia1*
1Affilated
Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，
Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China; *Correspondence should be addressed to hongxialiu04@yahoo.com.cn
Objective: Observation of the spleen jiedu decoction of pixu psoriasis sample mouse models of vascular
active factor and vaginal epithelial PCNA expression, this essay discusses the spleen decoction in the
treatment of detoxification of pixu wet type filled with psoriasis vulgaris mechanism. Methods: The
subject of three experiments, each experiment was randomly divided into control group, saline group，
A
capsules, spleen detoxification soup, low-dose group. Modelling for dual model, and 1-4 days for the
model of mouse spleen deficiency, 5-7 days while modelling in Spleen psoriasis-made mold, 8-13 days
for the drug or placebo intervention stage, 14 days sampling. To verify the Spleen model with psoriasis
model is successful, required from the blank group and the saline group in each experiment, randomly
selected 12 mice, the serum melatonin (MT) determination and vaginal epithelial cells cell mitosis
observed. After successful modelling on Spleen psoriasis mouse model of plasma endothelin-1, soluble
E-selectin were detected elements and vaginal epithelial PCNA. Result: 1、
A saline serum melatonin (MT)
level lower than the blank group, the two groups P<0.05，
from the laboratory point of view prompted
spleen deficiency model was successful. 2、
vaginal epithelial cells of normal saline group of mice the
number of mitotic higher than the blank group, suggesting that psoriasis successful modeling.3、
Spleen
detoxification soup to reduce the deficiency of the spleen psoriasis-like mouse model of plasma
endothelin-1，
soluble E-selectin in the role. Blank group and the saline group P <0.05；
A capsules and
spleen detoxification soup, low-dose group compared with the saline group P <0.05，
A capsules and
spleen detoxification soup high in the low dose group P> 0.05. Spleen detoxification soup, low-dose
group P> 0.05 .4、
spleen detoxification soup reduce the role of spleen deficiency type psoriasis mouse
model of vaginal epithelial PCNA expression. Between the groups overall P <0.01，
X 2= 43.815 by
pairwise comparisons between the sample Wilcoxon rank sum test (Nemenyi), a blank group and the
saline group P <0.01 with acitretin capsule group, the spleen detoxification soup in the high dose group
compared with P> 0.05, saline group with acitretin capsule group, spleen detoxification soup low in the
high dose group pairwise comparisons P <0.01，
A capsules, and spleen detoxification soup is low, middle
and high dose groups pairwise comparisons P> 0.05. Conclusion: The spleen jiedu decoction on plasma
44
endothelin 1 and soluble E and choose PCNA expression with benign adjust action, by lowering
endothelial cell activation degree, restrain psoriasis lesions formed in capillaries, reduce the cutin form
abnormal cellular proliferation and differentiation and so on various reach the purpose of psoriasis
treatment.
A-060
The Comparison on the Location of Insomnia According to Different Classification of
Traditional Chinese Medicine of Five-god Theory and Four Examinations
Fan Xiufang1, Zhang Xingping1
1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur
Autonomous Region, China
Objective: Study Chinese medicine insomnia five-God type and combination of all the typing consistency
in the positioning of the five internal organs, and provide clinical evidence of genotyping diagnostic
method of Traditional Chinese Medicine Insomnia five God. Methods: Subsidiary of the Chinese
Medicine Hospital sleep specialist expert outpatient collection of Xinjiang Medical University of insomnia
in patients with 204 cases, standardized collection of clinical information collection form, were the five
gods of the traditional Chinese medicine insomnia type and combination of all type, insomnia disease
established by genotyping the main orientation of the five internal organs, the establishment of
information database, the use of Kappa test methods to compare the type of Chinese medicine insomnia
five God with four diagnostic combination of all type consistency in the positioning of the five internal
organs. Results: the consistency of the five gods of the traditional Chinese medicine insomnia type and
combination of all typing on the five internal organs, Kappa, conformance test, kappa = 0.837 (P <0.001).
Conclusion: The type of Chinese medicine insomnia five God with four diagnostic parameter sub-type
high consistency in the positioning of the five internal organs.
A-061
The comparison research of acupuncture and acupoint application to patients with
AECOPD as well as the influence of lung function and life quality
Shao Meimei1, Liu Zhiyan1
1
Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China
Objective: To observe influence of acupuncture and acupoint application therapy in lung function of
patients with acute exacerbation of chronic obstructive pulmonary disease compared to the improvement
of clinical symptoms, quality of life and difficulty in breathing. Methods: 60 patients who in accordance
with the inclusion criteria were divided into four groups, including the control group (non-acupoint
acupuncture and simulated acupoint application)、
acupoint application group (plus non-acupoint
acupuncture)，
acupuncture group (plus simulated acupoint application), acupuncture plus acupoint
application group，
each group has 15 cases. The four groups were treated with conventional Western
medicine treatment, once a day, and 15 days as a course of treatment. Observed and record before and
after treatment respectively, which are lung function index (FEV1/FVC, FEV1%)、
TCM symptom
score、
quality of life score (CAT scale)、
difficulty in breathing of grading (MRC scale). Results: the group of
45
acupoint application in improving cough, expectoration, wheezing was statistically significant (P <0.05);
acupuncture group in improving cough, expectoration, wheezing, shortness of breath aspects are
statistically significant (P<0.05); and two associated with a significant effect (P <0.01);In aspect of
improving lung function in AECOPD patients, the quality of life scores, difficulty in breathing scores:
acupuncture group and acupoint application group after treatment, both improved (P <0.05), however, the
effect of acupuncture combined with acupoint application group is the best (P<0.01), and better than the
other three groups. Conclusion: The results confirmed the effectiveness of acupuncture and acupoint
application in the treatment of AECOPD patients, showed that both improve pulmonary function in
patients, to improve the acute symptoms、
breathing difficulties and improve the quality of life is meaningful,
and combination of two is more effective than simple acupuncture and acupoint application.
Take
Western medicine treatment of chronic obstructive pulmonary disease as a base, on the use of
acupuncture, acupoint application therapy can not only improve the clinical efficacy but also convenient
and safe.
A-062
The effect of jianpijiedu decoction on the vascular active factor of psoriasis model mice
Gao Yuan1, Liu Hongxia1*
1Affilated
Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China; *Correspondence should be addressed to hongxialiu04@yahoo.com.cn
Objective: To observe the plasma endothelin-1 and serum soluble E - select element level after giving
jianpijiedu decoction to psoriasis model mice and to discuss the effect and mechanism of jianpijiedu
decoction for curing psoriasis so as to provide experiment basis for clinical applications. Method: In
experiment 1, using the radiation immune method for determining the content of plasma endothelin-1, the
psoriasis model mice were divided into blank group/ model group /Chinese medicine group and control
group, in addition to blank group, all the groups are injected Diethylstilbestrol Diphosphate Inj three
consecutive days, then giving the Sodium Chloride /jianpijiedu decoction and Acitretin capsules to blank
group, Chinese medicine group and control group respectively for 6 days , to use pick eyes method to
take about 1 ML blood for determination before lethal injection of colchicin. In experiment 2, using the
ELISA method for determining the content of Serum soluble E - select element, the model mice were
divide into blank group/ model group /Chinese medicine group and positive control group, in addition to
blank group, all the groups are injected Diethylstilbestrlo Diphosphate Inj three consecutive days, then
giving the Sodium Chloride /jianpijiedu decoction and Acitretin capsules to blank group,/Chinese medicine
group and positive control group respectively for 6 days , to use pick eyes method to take about 1 ML
blood for determination before lethal injection of colchicin. Result: In two experiments, both jianpijiedu
decoction and Acitretin capsules can reduce plasma endothelin-1and soluble E - select element level
,Chinese medicine group was better than model group(P<0.05), positive control group was better than
model group(P<0.05), model group was better than blank group(P<0.05), There was no significant
differences between the Chinese medicine group and Acitretin capsules group in reducing plasma
endothelin 1 level.（
P>0.05）
F:\Dict\resultui\queryresult.htmlConclusion: The effect of jianpijiedu
decoction in treating psoriasis might by means of blocking multiple Onset link, so the content variation of
Plasma endothelin-1 and serum soluble E - select element may serve as Pharmacodynamic index to
46
provide experiment basis for clinical applications.
A-063
The Experiment of Jian Pi jiedu decoction on Guinea Pig Model of Psoriasis with Spleen
Deficiency Syndrome
Guo Fei1, Liu Hongxia1*
1Affilated
Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China; *Correspondence should be addressed to hongxialiu04@yahoo.com.cn
Objective: To establish the Guinea Pig Model of Psoriasis with Spleen Deficiency Syndrome and study
the function as well as dose-effect relationship of jianpijiedu decoction. Methods: Guinea Pig were
divided into 8 groups by randomization, blank 1group and blank 2group,experimental 1group and
experimental 2group with 10 Guinea Pig in each group; Chinese herbs high dose ,the medium-dosage
group and low dose groups, control group, with 12 Guinea Pigin in each group. Except blank 1 group and
blank 2group, the experimental 1group established the psoriasis model with 5% propranolol emulsion,
other groups after successfully establishing the psoriasis model with 5% propranolol emulsion, choose to
give gavage of bitter and cold purgative rhubarb twice a day for a continuous duration of 7d to injure the
spleen and stomach in order to duplicate a clinical like spleen deficiency model, then with ELISA test kit
for the guinea pig serum TNF alpha IL-20 content changes, conduct visual inspection and pathological
section observation on ear skin lesions among guinea pigs of all groups. Result: (1) 5% propranolol
emulsion brought out thepsoriasis-like lesion ,after established the model, experimental 1group and
blank1group has significant difference on baker grade (p<0.01);after give gavage of rhubarb,
experimental1 group and blank 1group has significant difference on spleen deficiency syndrome grade
and weight（
p<0.01）
;(2)jianpijiedu decoction has effect on guinea pig serum TNF alpha and IL-20 content,
after give gavage of jianpijiedu decoction and Acitretin capsule respectively, the serum TNF alpha and
IL-20 content of the high dose and the medium-dosage Chinese herbs groups became lower compared
with experimental 2 group（
p<0.01）
,the varying concentrations of the herbal medicine showed the obvious
dose-effect relations.(3)jianpijiedu decoction has effect on psoriasis-like histopathological and ,after give
gavage of jianpijiedu decoction and Acitretin capsule respectively, the lesion histopathological were
improved to some extent of the three Chinese herbs and control group and there has significant difference
compared with experimental2 group （
p<0.01）
,the result showed the higer of the dose and the better of the
effect, and the Chinese herbs high dose group better than control group（
p<0.05）
(4) jianpijiedu decoction
has improvement on Spleen Deficiency Syndrome, control group and experiment 2 group has no
significant difference on weight（
P>0.05）
,three Chinese herbs has significant difference(p<0.05 ） .
Conclusion: (1) The psoriasis spleen deficiency syndrome group preliminarily has the pathological
features of psoriasis, which are similar to TCM spleen deficiency syndrome symptoms.(2) jianpijiedu
decoction has effect on psoriasis may related control its inflammatory reaction and immune
adjustment;(3)jianpijiedu decoction not only improve the psoriasis-like lesion but also improve the spleen
deficiency syndrome.
A-064
47
The Experimental Study of Huangqi Guizhi Wuwu Decoction therapy on Failed Back
Surgery Syndrome
Liu Kaiying1, Huang Yifei1
1Affilated
Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China
Objective: To study the effectiveness and mechanisms of Huangqi Guizhi Wuwu Decoction on
preventing post laminectomy fibrosis and adhesion and to prevent failed back surgery syndrome effect.
Methods: 95 adult rats were divided randomly into five group, each 19. A group: Nacl group, B group:
Huangqi Guizhi Wuwu Decoction group, C group: Huangqi Guizhi Wuwu Decoction Plus invigorating the
blood and removing blood stasis Chinese Medicinal Herbs group, D group: Huangqi Guizhi Wuwu
Decoction group Plus resolving dampness Chinese medicine Herbs group, E group: Huangqi Guizhi
Wuwu Decoction Plus resolving dampness and activating blood circulation Chinese medicine Herbs. All
the rats were established as the experimental models undergoing L1 laminectomy with 0.3cm×0.8cm
dura mater exposed. All laminectomy levels were treated with relative medicine for four weeks: In the
treatment of at least 4, 8, 12 weeks, each group were sacrificed 7. To take paragraph spine with Lawson
standards to evaluate adhesion degree and detect the content of hydroxy proline of the animal epidural in
the scar tissue, and the groups were compared. Results: Compared with A, content of general
observation in group E group A degree of adhesion significantly increased in 4, 8, 12 weeks, a significant
difference(P<0.05). Compared with A, content of hydroxy proline of the epidural in the scar tissue of B, C,
D, E group were decreased within 8、
12 weeks were significantly different(P<0.05); At the 12th week，
E
group of hydroxy proline content not only less than A group(P>0.05) and B,C,D groups also significant
differences(P>0.05).Compared with the 4th week, the content of hydroxy proline of the epidural in the scar
tissue within 8, 12 weeks were significantly different (P<0.05). Conclusion: The drugs of Huangqi Guizhi
Wuwu Decoction could reduce scar formation and fibroblasts, lessen fibro scar and inhibit the formation of
adhesion in rats.
A-065
The Impact of Yangyin Huowei Heji on Chronic Atrophic Gastritis Patients' Gastro scope
Histopathology
Ding Guoning1, Zeng Binfang1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region
China; *Correspondence should be addressed to xjzbfang@yahoo.com
Objective: By using scaling biopsy technique, this thesis evaluates the effectiveness of Yangyin Huowei
Heji on chronic atrophic gastritis objectively. Figure out Yangyin Huowei Heji's effection on chronic
atrophic gastritis treatment, and verify the effectiveness of Xinjiang traditional Chinese medicine on
chronic atrophic gastritis. Methods: Using a randomized controlled trial, in the group of patients with
chronic atrophic gastritis, divided them into treatment group and control group. The treatment group was
given oral self Yangyin Huowei Heji, the control group was given an oral Weifuchun pills. Comparing the
improvement of Yangyin Huowei Heji and Weifuchun on chronic atrophic gastritis by using endoscopy,
histopathology and Hp before and after the treatment. Results:1.In gastroscopic observation, the two
48
groups in gastroscopy stomach mucosa degree has statistics difference after treatment (P<0.05); but the
treatment group and the control group comparison showed no significant difference (P >0.05);2.In
histopathology, two groups of patients with gastric mucosal degree, the extent of intestinal metaplasia and
dysplasia degree showed statistical difference after treatment (P<0.05), the two group in improving
glandular atrophy is also had statistical difference (P<0.05); 3. The Hp of the treatment group and the
control group has significant difference after treatment (P<0.05); but comparison between the two groups
shows no statistical difference (P>0.05). 4.In the comprehensive curative effect, total effective rate of the
treatment group is 79%, the control group is 73%, comparison between the two groups shows statistical
difference (P<0.05);5.Symptoms and signs of treatment group prove efficiency with no statistical
difference (P>0.05). Symptoms and signs of control group prove efficiency, there was statistically
significant difference (P<0.05). Conclusion: Yangyin Huowei Heji can not only effectively treat chronic
atrophic gastritis and be resistant to Hp infection, but also can repair of gastric mucosa.To a certain
extent, it also can reverse glandular atrophy, intestinal metaplasia and dysplasia, which can also prevent
chronic atrophic gastritis from developing to cancer. In different syndrome types, Yangyin Huowei Heji
has a satisfactory therapeutic effect. The control group in the stomach yin is deficiency, and in blood
stasis of stomach meridian treatment effect is poorer.
A-066
The Research on the Inhibition of Invasion and Metastasis of Colon Cancer Cells
HCT116 after the Administration of YiQiFuShengFang by ERK/MAPK Signaling Pathway
Han Jing1, Lv Shuqin1
1
Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China
Objective: To research the interaction mechanisms of anti-invasion and metastasis of tumor after using
YiQiFuShengFang on HCT116 though Mitogen acttiveated protein kinase /Extracellular signal-regulated
kinase signaling pathway, providing some basis of scientific experiments of anti-invasion and metastasis
of tumor in using Traditional Chinese Medical prescription. Methods: The proliferation of colon cancer
cells HCT116 was observed by methylthiazolyl tetrazoliym (MTT) assay. Select the appropriate drug
density to carry on the next step of the experiment. The effect of the migration of HCT116 cells that
treated by YiQiFuShengFang was examined by Scratch test. ELISA method was adopted to test the
express of vascular endothelial growth factor (VEGF) on HCT116 cells after using drugs. ELISA method
was adopted to test the express of Matrix metalloproteinase-2(MMP-2) on HCT116 cells after using
drugs. The cell cycle and apoptosis of HCT116 cells that conducted by YiQiFuShengFang were
examined by Flow Cytometry(FCM). P-ERK1/2 expression was determined by Western-blot, in that was
the activation of ERK1/2 which was measured by the extent of ERK phosphorylation. Results: MTT
assay showed the proliferation of colon cancer cells HCT116 could be inhibited
by YiQiFuShengFang. YiQiFuShengFang inhibited the migration of HCT116 cells. YiQiFuShengFang
reduced the expressing of VEGF, meanwhile,it cut down the expressing of MMP-2. Through the analysis
of flow cytometric, the apoptosis of tumor cells were significantly higher and cell cycle was changed.
Western-blot assay showed that ERK1/2 phosphorylation was suppressed by YiQiFuShengFang, that is
ERK1/2 could be inactivated by the down regulation of P-ERK1/2. Conclusion: YiQiFuShengFang
inhibits the proliferation of colon cancer cells hct116，
down-regulates the expression of VEGF and
49
MMP-2，
and induces the apoptosis of cancer cells which may be mediated by MAPK/ERK signal pathway.
A-067
The Study on Therapeutic Effect of Inflammatory Factors on DangGuiNianTongTang
Pasty with MTX on Adjuvant Arthritis in Rats
Yuan Li1, An Liping1
1
The First Affilated Hospital, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region , China
Objective: Observing Danggui Niantong Tang pasty’s affection by the adjuvant arthritis (AA), the
apoptosis of synoviocytes, serum cytokines and other indicators. Which explore the treatment of
rheumatoid arthritis in rats about DangGuiNianTongTang Pasty, with the oral administration of
methotrexate(MTX), and compared with MTX +Diclofenac Diethyl amine Emulgel, to explore the early
control of disease progression in topical ointments, and a better understanding of DangGuiNianTongTang
Pasty and Diclofenac Diethylamine Emulgel’s difference on relevant indicators and symptoms of
inflammation improve. Methods: (1) Selected wistar rat 48, according to the random number table they
were divided into blank group, model group, treatment group (MTX + Danggui Niantong Decotion of
application), and the control group(MTX+ Diclofenac Diethylamine Emulgel), n=12, and number, in
addition to the blank group, each rat’s right toe skin injected Freund’s complete adjuvant 0.1ml
proinflammatory. (2)Two weeks after the modeling, all rats by intragastric administration of MTX and
gauze wrapped drugs and topical administration , administration of a total of 18 days, blank group not be
deal with, the model group were given topical poured into distilled water and a simple gauze comfort
therapy. (3)Observe the general situation of the rats before and after the modeling, measured by ELISA
in IL-17, TNF-ɑ, and take the right side of the knee joint cavity within the synovival tissue, and processed
into single cell suspension, add the appropriate reagents, and flow cytometry immediately. Results:
(1)The treatment group’s TNF-ɑ, IL-17 levels lower than the model group (P<0.05), the control group’s
TNF-ɑ, IL-17 levels lower than the model group (P<0.05), the treatment group’s TNF-ɑ, IL-17 level slightly
higher than the control group (P<0.05). (2)The treatment group’s synovial cell apoptosis rate was higher
than the model group (P<0.05), apoptosis of synovial cells in the control group higher than the model
group(P<0.05), synovial cell apoptosis rate of the treatment group slightly higher than the control
group(P<0.05). Conclusions: (1)The DangGuiNian TongTang Pasty with MTX had good local
antinflammatory and analgesic effect, inhibit autoimmune inflammation,restore the abnormal immune
regulatory function. (2)The DangGuiNianTongTang Pasty with MTX had a significant effect in improving
the rate of apotosis of synovial cells.
A-068
Research on the combination of Acupuncture and Auricular Acupuncture treatment for
patients with Acute Exacerbation of COPD
Cao Lin1, Liu Zhiyan1
1Affilated
Traditional Chinese Medicine Hospital, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous
Region, China
Objective: Observe the influence of life quality and the improvement of pulmonary function with
50
acupuncture combined with auricular acupuncture therapy on patients with acute exacerbation of COPD.
Methods: 60 patients with acute exacerbation of COPD were randomly divided into four groups:
acupuncture combined with auricular acupuncture group, acupuncture group (acupuncture combined with
simulate auricular acupuncture), auricular acupuncture group (simulate acupuncture combined with
auricular acupuncture), control group (simulate acupuncture combined simulate auricular
acupuncture).Four groups received conventional treatment as basis combined with acupuncture,
auricular acupuncture, simulated acupuncture, simulated auricular acupuncture. After two courses (20
days) of treatment, observe FEV1, FEV1/FVC ratio of pulmonary function and CAT, MRC, as well as
traditional Chinese medicine symptom scale quality of life scores of the four groups of patients before and
after treatment. Results: The lung function: There was statistically significant (P<0.05) of patients with
pulmonary function in four groups before and after treatment. acupuncture combined with auricular
acupuncture group’s FEV1、
FEV1/FVC ratio improved compared with before treatment, the improvement
of pulmonary function was better than the other three groups; CAT scales reflect the quality of life score
decreased significantly compared with before treatment, acupuncture combined with auricular
acupuncture group was better than the acupuncture group and auricular acupuncture group, and was
statistically significant compared with control group (P<0.05). Conclusion: Acupuncture combined with
auricular acupuncture for treating acute exacerbation of COPD patients with the pattern of phlegm
obstructed in lung, which is effective in the clinical symptoms, especially the improvement of airflow
limitation as well as difficulty in breathing. Four groups compared with each other, suggesting that
acupuncture, auricular acupuncture can treat COPD, the combination of their two is better, and patients’
quality of life also improved.
A-069
Research on Traditional Chinese Medicine theory and gynaecological diet therapy
Tan Rong1, Liu Ailing1
1
Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China
Objective: This subject is relevant to the diet of traditional Chinese medicine or card literature information
collection and filing. TCM literature information gynecological dietotherapy comb, and discusses
gynecological famous dietotherapy formulae rule, screening by common gynecologic with births suitability
dietotherapy method and formulas, for Chinese medicine clinical application and gynecological
dietotherapy formulas provide literature development foundation. Methods: the method of literature
research system, the han dynasty to Ming and qing dynasties access each time the diet of traditional
Chinese medicine (TCM) or certificate of literature material, original literature information identification,
collection and management and layout, this study prescriptions altogether collected 761 songs,
postpartum disease prescriptions dietotherapy 280 songs, accounting for 36.79% of the total number of
prescriptions collection, pregnancy illness prescriptions dietotherapy 276 songs, accounting for 36.27% of
the total number of prescriptions collection, menstrual disease diet formula 117 first, accounting for
15.37% of the total number of prescriptions collection, taken down to disease first 45, accounting for
5.91% of the total number of prescriptions collection, miscellaneous diseases and first, accounting for
6.04% of the total number of prescriptions collection. Among them the first 216 han the sui and tang
dynasties, accounting for 28.38% of the total number of prescriptions collection; 227 the first jing. Collect
51
accounts for 29.83% of the total number of prescriptions, the Ming and qing dynasties 318 first,
accounting for 41.79% of the total number of collection; Postpartum disease of diet in the first 280
prescriptions, han the sui and tang dynasties first 92, accounting for 32.86% of the total number of
prescriptions postpartum, 86 the first song, accounting for 30.71% of the total number of prescriptions
postpartum disease, the Ming and qing dynasties 102 first, accounting for 36.43% of the total number of
prescriptions postpartum disease; The first pregnancy illness 276 prescriptions diet, han the sui and tang
dynasties first 87, accounting for 31.52% of the total number of prescriptions pregnancy illness, the song
first 83, accounting for 30.07% of the total number of prescriptions pregnancy illness, the Ming and qing
dynasties 106 first, accounting for 38.41% of the total number of prescriptions pregnancy illness.
Application of frequency analysis, variance analysis methods, combined with traditional Chinese
medicine logic reasoning and analogy method, formula metrology research methods, traditional Chinese
medicine is the biggest of the limits using drug that diet card, etiology comb and statistical analysis.
Results: the gynecology in having to prescriptions diet drug, remove blood stasis drugs, XieBiao
medicine, heat the water permeability of drug, wet medicine, hemostatic medicine, the temperature of
drug, astringent medicine, medicine this ten kind of drug regulating the accumulative total of frequency up
to 92.34%, is the main medicine of traditional Chinese medicine of gynecological diet. Among them,
having medicine, remove blood stasis drugs, XieBiao medicine, heat the water permeability of drug, wet
medicine, hemostatic medicine, temperature are the most commonly used in medicine, seven of the
cumulative frequency of 86.99%, in clinical therapy, plays a main role. Compensation in medicine to fill
gas, blood, filling Yang drug is given priority to, accounting for 87.28% of the benefit drug use frequency,
four gas temperature, and flat, cold three kinds of the cumulative frequency drugs reached 90.02%,
traditional Chinese medicine is a gynecological formula of four gas main diet drugs. Among them the use
frequency of chiral drugs temperature highest 43.13%, five in gansu and Michael essien, bitter three kinds
of the cumulative frequency drugs reached 89.54%, traditional Chinese medicine is a gynecological
formula of five main diet drugs. Among them with our drug use, the highest frequency, 49.97%; Be in the
spleen, heart, lungs, liver, kidney, stomach six drugs cumulative frequency to 94.89%, traditional Chinese
medicine is a gynecological diet formula of the main to drugs. Among them with the drug use to spleen
highest frequency, 21.13%. In all of poisonous drug prescription diet is occupied 9.67%, the drug toxicity
is 90.63%. Gynecology in common use in the form of prescription diet is: one, and the wine agent,
powder, pills, food, beverage, porridge agent. Respectively 39.68%, 14.85%, 14.06%, 8.94%, 6.18%,
5.91%, 4.47%. Conclusion: the gynecology diet drug prescriptions range, effect of drug, having to
remove blood stasis drugs, XieBiao medicine, heat the water permeability of drug, wet medicine,
hemostatic medicine, temperature are the most commonly used in medicine to give priority to. Commonly
used drugs have wine, angelica, ginger, born tuberous root, liquorice, root of herbaceous peony,
cinnamon, ginseng, astragalus membranaceus, poria cocos, ligustici chuanxiong rhizoma, white
donkey-hide gelatine, lamb, honey, largehead atractylodes rhizome, worship singing, big jujube,
mugwort, dwarf lilyturf, commonly. General use in gansu and Michael essien, taste bitter primarily,
medicinal properties to temperature, flat, cold Lord; Return to the spleen, heart, lungs, liver, kidney,
stomach primarily. In one form, and the wine agent, powder, pills, food, beverage, porridge agent mainly.
A-070
Distribution characteristics of TCM syndrome and treatment on 2026 Cases of Sub-health
52
Population of Urumqi City
Du Shuai1, Hu Xiaoling1*
1Affilated
Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China, *Correspondence should be addressed to xjhuxl @yahoo.com.cn
Objective: through the investigation of Urumqi city part of sub health and sub-health population
distribution characteristics of TCM syndrome of TCM diagnosis and treatment, clinical program laid the
foundation. Methods: by means of questionnaire survey Urumqi city on 2026 cases of sub-health
population distribution and the characteristics of TCM syndrome analysis. Results: (1) with sub health
diagnosis criteria of 2026 people, including 1052 males,51.92% females,974 people, accounting for
48.08%;1725 Han Chinese people, accounting for 85.14%,301 minority people, accounting for 14.86%;
married1533, accounting for 75.66%,371 unmarried people, accounting for 18.31%,
divorced/(widowed)122 people, accounting for 6.03%; occupation, teachers, administrative staff,
technology and engineering researchers, medical personnel, administrative personnel accounted for
78.61%, workers and business service personnel as well as other accounted for 21.39%; cultural degree
with bachelor degree or above more accounted for 57.74%, college education accounted for 42.26% of
the following. (2) the distribution of TCM Syndromes of sub health state with Yin deficiency of liver and
kidney syndrome of phlegm heat disturbance to maximum, minimum,8card form than the sequence is:
liver-kidney Yin deficiency, spleen deficiency syndrome (21.84%) two (16.97%) > liver fire syndrome of
Liver-qi Depression Syndrome (14.21%) > (13.72%) > liver stagnation and spleen deficiency card >
spleen deficiency and dampness stagnation syndrome (11.50%) > lung spleen qi deficiency syndrome
(5.62%) > phlegm heat disturbance syndrome (4.44%). Women with Yin deficiency of liver and kidney
syndrome and syndrome of stagnation of Liver-qi see more, men with Yin deficiency of liver and kidney
syndrome, heart and spleen deficiency syndrome in two, Han Yin deficiency of liver and kidney, heart
lienal two empty; minority with Yin deficiency of liver and kidney, spleen deficiency and dampness,
phlegm heat disturbance mainly. Yin deficiency of liver and kidney syndrome is more concentrated in
the40~49 years old. Various types of age, gender, ethnic aspects of the sidents in sub-health state has its
own characteristics, sex, age, nationality on differences were statistically significant (P<0.05).
Conclusion: Urumqi city re the sub health are affected, the future of sub health status of Chinese
medicine intervention to lay the foundation.
A-071
The research on life quality of Yang-yin-huo-wei Formula Treatment of Chronic Atrophic
Gastritis Patients
Dai Ming1, Zeng Binfang1*
1
Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region ,
China; *Correspondence should be addressed to xjzbfang@yahoo.com
Objective: To observe the Yang-yin-huo-wei formula treatment of chronic atrophic gastritis on patients
report outcomes and quality of life effects. Methods: Select the Xinjiang Uygur Autonomous Region
Chinese Medicine Hospital Gastroenterology Clinic in August 2010 to January 2012 207 Patients who
was up to diagnostic criteria were randomly divided into treatment group and control group. Were 104
53
cases of the treatment group and control group (n=103), the treatment group was treated with
Yang-yin-huo-wei Formula (one dosage daily, 2 times, morning and evening after meal), while the control
group was treated with Weifuchun (3 times daily, 4pills each time). Quantitative score and statistical
analysis to compare clinical outcomes and quality of life reported by patients before and after treatment,
respectively. Result: (1) 5 overall dimensions of the PRO scale comprehensive comparison of the
treatment group points lower than the control group (F=12.824,P=0.000<0.05); Single factor analysis of
variance for each dimension, including partial-stomach feeling, the overall body condition, and
gastrointestinal symptoms after the three dimensions of the treatment group scores lower than the control
group (P<0.05), Integral in two aspects of the psychological state and social health was no significant
difference (P>0.05); Scale evaluation of 30 patients reported clinical outcomes after treatment of the
state, the treatment group, epigastric pain, epigastric noisy, pantothenic acid, heartburn, dry mouth, 9
entries after treatment, scores were lower than control group (P<0.05); Difference in the scale of 30
patients report clinical outcomes before and after treatment, higher than in the nine entries in the
treatment group difference of pantothenic acid, heartburn, dry mouth, epigastric fullness, epigastric pain,
epigastric Work for noisy, the control group (P<0.05). (2) Treatment group and control group after
treatment, quality of life scores were higher than before treatment (P<0.05), to improve the quality of life in
patients. Two groups of patients to treatment group in addition to treatment in this dimension of the
emotional functions (RE), higher than that of the control group (t=-2.33, P=0.021); in the other seven
dimensions with the control group no significant difference (P>0.05). The difference between the two
groups of quality of life in addition to the dimension of the mental health (MH) difference was statistically
significant (t= 2.45,P= 0.015 <0.05), and the treatment group was higher, while in the other
seven-dimensional degree has had no statistically significant (P<0.05). Conclusion: (1)Yang-yin-huo-wei
formula a good therapeutic effect of atrophic gastritis, improve the clinical outcomes of patients reported
overall better than Weifuchun, mainly reflected in the local epigastric feeling, body and the overall state of
gastrointestinal symptoms in three aspects. Among them, the nine entries of epigastric pain, epigastric
noisy, pantothenic acid, heartburn, dry mouth, such as therapeutic effect obvious while in epigastric
fullness, epigastric pain, epigastric noisy, pantothenic acid, stomach burning sensation, dry mouth, and
other nine entries in the degree of improvement is obvious. (2) Yang-yin-huo-wei formula and Weifuchun
can improve the quality of life of patients, while no significant difference between in the two (P>0.05).
A-072
Anti-hypertensive effects of the aqueous extracts of Chinese yam (Dioscorea opposita
Thunb.）
in Two-Kidney, One-Clip Renal Hypertension Rat model
Nurmuhammat Amat1, Palidan Wushuer2, Sajida Abdureyim3, Dang Ming4, Dolkun Mamut 5*, Anake
Kijjoa 6
1Traditional
Uighur Medicine Institute, Xinjiang Medical University, 830011, Urumqi, Xinjiang, PR China;
2
Psychosomatic
Department of Traditional Chinese Medicine Hospital, Xinjiang Medical University, 830011，
Urumqi, Xinjiang, PR China; 3First
Affiliated Hospital, Xinjiang Medical University, 830011，
Urumqi, Xinjiang, PR China; 4Postgraduate College, Xinjiang Medical
University, 830011，
Urumqi, Xinjiang, PR China; 5Salamat Biotechnology Co., Ltd. Xinjiang, China; 6ICBAS - Instituto de Ciências
Biomédicas de Abel Salazar and CIMAR, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto,
Portugal.*Correspondent to dolkunmamut@hotmail.com or Dr Nurmuhammat Amat, nur818@hotmail.com.
Objective: Chinese yam (Dioscorea opposita Thunb.) is a medicinal herb used in traditional Chinese
54
medicine to treat different system diseases including hypertension. The objective of the study was to
investigate the possible antihypertensive effects of Chinese yam (CY) extract in renovascular
hypertensive rats as well as the mechanism in reducing blood pressure. Methods: The two-kidney
one-clip (2K1C) Goldblatt model of renovascular hypertension was used in Wistar rats. Rats were
randomized into sham, untreated 2K1C, captopril-(30mgkg−1 per day orally) and CY-(210, 420mgkg−1
per day orally)，
medium (M)-CY, high (H)-CY treated 2K1C groups (n=10-12 per group), followed up for 6
weeks. The Blood pressure, cardiac mass index (heart weight/body weight), plasma level of angiotensin-II
(Ang-Ⅱ
), endothelin-1(ET-1), superoxide dismutase (SOD) and malondialchehyche (MDA) were
evaluated. Results: CY significantly reduced mean systolic and diastolic blood pressure after treatment
(P<0.05). CY also significantly increased plasma SOD activity, decreased plasma MD concentration.
Renal function was improved with captopril and CY. Ang-Ⅱ
was reduced by CY to same extent with
captopril. ET was reduced to lower concentrations (74.79 ± 17.88, 90.75 ± 39.31pgml−1 for L-, H-CY,
respectively) than in sham (116.12 ± 39.34pgml−1), untreated 2K1C (170.19 ± 39.34pgml−1) or captopril
(68.22 ± 22.10pgml−1) groups. They also significantly reduced the left ventricular hypertrophy and
cardiac mass index. These findings are in agreement with the traditional use of CY in the treatment of
arterial hypertension. Conclusion: Our results also suggest that CY may have an antihypertensive
effects on hypertension by inhibit ET-converting enzyme and antioxidant activity, and warrant further
exploration.
A-073
Effect of TongFeiJingTanFang on Immunologic Function of Phlegm Heart Syndrom in
Chronic Obstructive Pulmonary Disease
Bai Zhengping1, Tan Guangbo1, Hu Xuejun1
1Affiliated
Hospital Combining Traditional Chinese & Western Medicine of Hunan University of TCM, Changsha, China
Objective: To observe the clinical effects and immunologic function of TongFeiJingTanFang in treating
phlegnr heart syndrome of chronic obstructive pulmonary disease (COPD). Methods: 114 subjects were
randomized into two groups：
58 cases were treated by not only conventional therapy but also
TongFeiJingTanFang (experimental group), and the remaining 56 cases were subjected to conventional
therapy (control group). The changes of the respiratory symptome , the scores of patients’life quality
including body mass index, St . George respiratory questionnaire, pulmonary function test, and 6-minute
walking distance, and levels of plasma immunologic function were compared between two groups. The
course was four weeks. Results: Total effective rate in the experimental group was better than that in the
control group (P<0.05). There was significant statistical differences between the two groups, which shows
that the experimental group was superior to the control group in amelioration of syndrome including cough
and sputum ,and the integration of SGRQ, increasing levels of CD4+、
CD4+/CD8+、
IL-2,decreasing
CD8+、
IL-8. Conclusion: TongFeiJingTanFang can allevate respiratory symptom, improve quality of life
and regulate immunologic function in patients who are phlegm heart syndrome of COPD.
A-074
Effects of “Yang-Warming and Kidney Essence-Replenishing Herbal Paste” on
55
Cold-related Asthma Exacerbation——A Report of 74 Cases
Tang Binqing1*, Shi Kehua1, Li Xiaoqi1, Wang Hongchang1, Fang Hong1, Xiong Bidan1, Wu Yingen1
1Shanghai
University of Traditional Chinese Medicine , Longhua Hospital, No. 725 Wan Ping South Road , Shanghai, 200032,
China; *Correspondence should be addressed to tangbinqing1979@163.com
Objective: To observe the effects of “Yang-Warming and Kidney Essence-Replenishing” herbal paste on
cold-related asthma exacerbation. Methods: A total of 151 cases of moderate to severe persistent
asthma (3 cases didn’t finish the expected treatment and 5 cases lost during follow-up) were randomly
divided into a herbal paste treatment group (74 cases) and a control group (69 cases). Both groups were
given basic treatment according to Global Initiative for Asthma (GINA) scheme and the treatment group
was also treated with herbal paste in winter. The onset frequency of asthma exacerbation,cold and
cold-related asthma exacerbation, ACT score and kidney-deficiency syndrome score within one year in
each group were observed. Results: Compared with control group, the onset frequency of asthma
exacerbation, cold and cold-related asthma exacerbation, and kidney-deficiency syndrome score were
significantly reduced（
P<0.01）
. There was no significant difference in ACT score between the two groups.
Conclusions: Receiving treatment of “Yang-Warming and Kidney Essence-Replenishing” herbal paste in
winter can reduce cold-related exacerbation of asthma.
A-075
Huang Di Nei Jing health theory and the prevention and treatment for chronic obstructive
pulmonary disease
Xu Yanling1
1Department
of Respirology, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Beiling Dajie No. 33,
Huanggu District, Shenyang 110032, Liaoning Province, China
Huang Di Nei Jing is one of the earliest books in the existing medical literature in China. It gives a
comprehensive overview of the system architecture of the theoretical system of Traditional Chinese
medicine (TCM), reflects the theoretical principles and academic thought of TCM, and it also laid the
foundation for the development of TCM. Huang Di Nei Jing is not only a great medical masterpiece, but
also a great book for health science and rehabilitation science. The “Preventive Treatment of
Diseases”idea it advocated and its wealth of health theory and experience are still of great significance for
the guidance of disease prevention and cure. In this study, we investigate the role of Huang Di Nei Jing
health theory in guiding the prevention and treatment of chronic obstructive pulmonary disease (COPD)
from harmony with nature, living a regular life with certain rules, regulating emotion, keeping a clear mind;
eating and drinking abstemiously; drugs and food simultaneously; sex abstemiously, keeping yin fluid,
focusing on prevention, preventive treatment of diseases. We emphasis that the daily lives of COPD
patients should follow the nature of yin and yang fluctuation law, should work and rest appropriately that
can not only reduce the number of acute attack, and also can alleviate the acute exacerbation. We attach
importance to the human spirit emotions and activities, balancing diet and sex abstemiously in the
prevention and treatment of COPD. Also, we advocate early prevention, early diagnosis and early
treatment of COPD. Finally, we concluded that using the Huang Di Nei Jing health theory to guide clinical
practice will bring to the overall improvement of the prevention and treatment of COPD and other chronic
56
diseases.
A-076
Influence of Compound Panax notoginsenosideGranules (CPNG) on bleomycininducedpulmonary fibrosis in rats
Ling Chunhua1, Wu Juanjuan2
1First
Affiliated Hospital of Suzhou University, 215006, Jiangsu Suzhou, China; 2Suzhou Chinese Medicine Hospital, 215006,
Jiangsu Suzhou, China
Objective: To explore the effects of Compound Panax notoginsenoside Granules (CPNG) on
bleomycin-induced pulmonary fibrosis in rats and its mechanisms. Methods: Sixty male Sprague-Dawley
rats were randomly divided into 6 groups, including control normal group，
model group, prednisolone
group(3.33mg·kg-1·d-1) and CPNG high-dose, moderate-dose and low-dose group
(100、
50、
25mg·kg-1·d-1), 10 Sprague-Dawley rats in each group. Except the control normal group,
pulmonary fibrosis models were reproduced by intratracheal injection of bleomycin(5mg/kg). On the
second day of injection of bleomycin, the rats in each treatment group were intragastric administrated
prednisolone and corresponding dose of CPNG daily; control normal group and model group were
intragastric administrated the equal volume distilled water daily. After 28 days, all rats were executed to
be observed the pulmonary index. Hydroxyproline (HYP) in lung tissue was assessed. Fixed parts of the
lung tissue was obtained for HE and Masson stain for histopathological examination. Malondialdehyde
(MDA), glutathion (GSH), superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) in blood
and lung tissue were respectively assessed by means of Thibabituric Acid(TBA), chemical colorimetry,
xanthine oxidase and chemical colorimetry. The expressions of transforming growth factor β1 (TGF-β1),
α-smooth muscle actin (α-SMA), Smad2, Smad3 and collagen I (Col-I) protein were analyzed by
immunohistochemistry. The expressions of TGF-β1, Smad2, Smad7 protein were analyzed by
western-blotting. Results: Compared with model group, the pulmonary index and lung tissue HYP were
decreased (P<0.05, P<0.01), MDA level in blood and lung tissue was also decreased(P<0.001), but GSH,
SOD, T-AOC level in blood and lung tissue were significantly increased (P<0.05, P<0.01, P<0.001) in all
of CPNG groups . Histological evaluation of lung specimens demonstrated that CPNG dramatically
decreased the lung inflammation and lung fibrosis in rats(P<0.01，
P<0.01). Munohistochemistry and
western-blotting demonstrated that CPNG could inhibite the expressions of TGF-β1, α-SMA, Smad2,
Smad3, Col-I protein(P<0.05, P<0.01, P<0.01), and increase the expression of Smad7 protein in lung
tissue of rats(P<0.01). Conclusion: CPNG has a therapeutic effect on bleomycin-induced pulmonary
fibrosis in rats, which maybe relate to antioxidative damage, intervention TGF-β1/Smads signaling
pathway, and restraining the formation and deposition of collagen.
A-077
Qufeng Xuanfei side clinical study of treatment of allergic cough
Xu Lihua1, Li Lei1, Huang Ye1
1Jiangsu
Provincial Chinese Medicine Hospital, 21002, Nanjing, China; 2Liyang City Hospital of Chinese Medicine Hospital,
213300, Liyang, China
57
Objective: To observe the clinical curative effect of the traditional Chinese medicine on atopic cough.
Methods: 60 cases with atopic cough patients were randomly divided into two groups. 30cases of the
treatment group, treated by the Chinese traditional curative methed. (medicine:
sangye、
qianhu、
baibu、
chanyi、
zhijiangcan、
pingdimu、
zhimahuan、
pipaye、
gaicao. Increase or deduce the
quantity of different medcine according to different causes of desease, eg: Wind and cold attacking the
lung syndrome, add fangfeng、
suye、
guizhi、
jiegeng; Wind and dryness attacking the lung syndrome add
maidong、
yuzhu、
nanshasen、
zhimu; Wind and heat attacking the lung syndrome add
yinhua、
sangbaipi、
lugen、
huangqin、
xingren；
a decoction per day).30 cases of the treatment group with the
treatment of loratadine, we need the observe the syndrome score and main symptom index of the
patients. Results: The total effective rate of treatment group is 86.67%, control group in the total
efficiency is 76.67% . The difference was statistically significant. The symptom scores were improved in
both groups, while the result of the treatment group is more obvious than that control group, with statistical
significance. Conclusion: Atopic cough syndrome belongs to Chinese traditional medicine, " feng Ke "
type, self-made “Qufeng Zhike” treatment can significantly improve symptoms of those who have atopic
cough and reduce the allergic level of the patients. Chinese traditional treatment has certain advantages
and is worthy of further study and researed.
A-078
Randomized Control Trial of JinhuaQinggan Granule in Treating Influenza with Wind-Heat
Affecting Lung Symptom-Complex
Li Guoqin1, Liu Zhiguo1, Liu Qingquan1
1Guang’anmen
Hospital affiliated to China Academy of Chinese Medical Sciences, 100053, Beijing, China
Objective: To observe the effect and safety of JinhuaQinggan Granule (JHG) in treating influenza with
wind-heat affecting lung symptom-complex (WHALS), besides study the optimal dosage of it. Methods:
Influenza patients with WHALS were enrolled from 6 clinical center of the study. They were randomized
divided into 3 group, high dosage group, low dosage group and the placebo group, then they receive 5
days medication before valuation. Results: 136 cases confirmed with influenza were enrolled from 992
patients, statistical analysis shows low dosage group of JHG significant reduced the estimated median
time to fever resolution compared with the placebo group, synthesize Chinese medical curative effect in
low dosage group is better that of placebo group at the 3th and 5th day from treatment. High dosage
group has a similar effect with low dosage group except inferior to partial criteria. There is no significative
difference among these three groups. Conclusion: JHG is effective and safety in treating influenza with
WHALS, Low dosage is the optimal dosage of JHG.
A-079
Scorpion - centipede on the rat model of asthma airway inflammation and airway
remodeling in experimental research
Li Haiyan, Yang Peilan, Huang Haiyin, Cui Jingang, Tang Jie, Wu Yingen
Objective: Observe the impact of “scorpion-centipede” on airway inflammation and airway remodeling of
asthmatic rats, to explore the mechanism of action of “scorpion-centipede” for asthma. Methods: Thirty
58
SD rats were randomly divided into three groups:normal control group,model control group, Chinese
medicine treatment group,10 in each group.The asthmatic rat airway remodeling model was established
by ovalbumin sensitized method, “scorpion-centipede” was applied to intervene. Preparation of
bronchoalveolar lavage fluid(BALF) and lung tissue biopsy harvested after three weeks of treatment. The
HE staining of the airway structure changes, and Masson staining of smooth muscle, collagen fibres, and
determination of the extent of the airway remodeling in medical image analysis software. Results:
Compared with normal group, number of total leukocytes and the percentage of eosinophil, neutrophil,
lymphocyte in BALF were significantly higher in the model group(P<0.01),the thicker of Bronchial wall,
smooth muscle layer and collagen fibres was significantly increased in the model group(P<0.01);
Compared with model group, number of total leukocytes and the percentage of eosinophil, neutrophil,
lymphocyte in BALF were significantly lower in the Chinese medicine treatment group (P<0.01),the thicker
of Bronchial wall, smooth muscle layer and collagen fibres was significantly decreased in the Chinese
medicine treatment group (P<0.01). Conclusions: Scorpion-centipede reduce airway inflammation of
asthmatic rats, plays an inhibitory role in airway remodeling by reduce the bronchial wall and smooth
muscle thickness, reduce the proliferation of collagen fibres.
A-080
The application of Lung and Large intestine Interacts relations in COPD
Geng Limei1
1 Hebei
Province Chinese Medicine Hospital, 050011, Shijiazhuang, China
Objective: To investigate the application and mechanism of Lung and Large intestine Interacts relations
in COPD. Methods: Objective 32 AECOPD patients with retention of phlegm heat syndrome in the lung.
The control group was treated with routine method. In the treatment group, we added Qingre Xiefu
Chinese traditional medicine. Result: Total effective rate of the Control group was 93.75 % and the
treatment group was 87.50%. There were obvious differences between the control group and the
treatment group (P<0.05). Conclusion: Qingre Xiefu Chinese traditional medicine was helpful to the
AECOPD patients with retention of phlegm heat syndrome in the lung. Lung and Large Intestine theory of
clinical value in COPD was worthy of promotion.
A-081
The effect of Er Chen Jie Han Particle for the treatment of OSAHS patients with Phlegm
Damp Syndrome
Wang Zhen1, Yang Junchao1, Shen Yingying1, Xu Tingzhen1
Objective: To appraise the effect of Er Chen Jie Han Particle for treatment of OSAHS patients with
phlegm damp syndrome. Methods: 80 patients of the outpatient and ward，
in the form of envelopes，
were
randomly divided into two groups．
Control group，
40 cases，
were treated with a healthy lifestyle for 3
months．
Treatment group，
40 cases，
were treated with Er Chen Jie Han Particle on the basis of a healthy
lifestyle for 3 months．
Before and after the end of the treatment，
patients were observed blood lipids、
body
mass index（
BMI）
、
blood viscosity、
polysomnography detection（
PSG）
、
Epworth sleepiness scale、
Quality of
life. Results: 1.Compared with before treatment，
BMI of control group had obvious improvement (P<0.05),
59
TG、
CHOL and BMI of TCM group had obvious improvement (P<0.05)；
2.Compared with before
treatment，
low shear of whole blood viscosity of control group had obvious improvement (P<0.01), both
low and middle shear of whole blood viscosity of TCM group had obvious improvement (P<0.01). After
treatment, compared with the control group，
low shear of whole blood viscosity of TCM group showed
significant difference (P<0.01)；
3.Compared with before treatment，
both the lowest oxygen saturation and
Quality of life of control group had obvious improvement (P<0.01 or P<0.05)，
AHI、
the lowest oxygen
saturation、
Epworth sleepiness scale and Quality of life of TCM group had obvious improvement(P<0.01 or
P<0.05. After treatment, compared with the control group，
the lowest oxygen saturation、
Epworth
sleepiness scale and Quality of life of TCM groups showed significant difference (P<0.01). Conclusion:
On the basis of healthy living，
Er Chen Jie Han Particle can lower BMI、
blood lipids and blood
viscosity，
significantly improve low ventilation and hypoxia，
reduce daytime sleepiness symptoms，
improve
quality of life of OSAHS patients with phlegm damp syndrome．
A-082
The search of basic law of the ancient physicians using the prescription medication to
treat "Pulmonary heart disease in the remission stage"
Hu Xuejun1, Bai Zhengping1*, Tan Guangbo1, Gan Rong 1, Liu Min 1
1Affiliated
Hospital of Hunan Province Institute of Traditional Chinese Medicine, Changsha 410006, China; *Corresponding
authors: Affiliated Hospital of Hunan Province Institute of Traditional Chinese Medicine, Hunan Yuelu District, Foothills No. 58,
tel+86-13873100306，
tel +86-0731-88904052, 410006, China
Objective: To understand the basic law of the ancient physicians using the prescription of medication to
treat “Pulmonary heart disease in the remission stage ”. Methods: Representative from the 30 remnants
of Chinese ancient books, to collect the prescriptions, Chinese material medica, the dosage and the
amount of content that the ancient physicians using to treat “Pulmonary heart disease in the remission
stage”. And the content were classificated, statisticed, analyzed and summarized. Results: Sorting out
the ancient prescriptions 110, involving more than 10 types of medicines, the main treatment method of
14 kinds. Tonifying drugs accounted for 38.2% totally. Drugs of reducing phlegm, relieving cough
and asthma accounted for 13.3% totally, the second is the diaphoretic, the heat-clearing drugs,
damp-clearing drugs,qi-regulating drugs,astringent drugs, interior-warming drugs, blood-activating drugs,
aromatic damp-resolving drugs, etc. Conclusion: when the ancients cure this disease, they often
tonificate and purgate in combination, taking tonification as the main, tune up the lung, spleen and kidney
together, to tonify the qi is the first, and pay more attention to reduce phlegm, to relieve cough and
asthma.they took the ways of clearing the surface, clearing the heat, regulating qi, invigorating the blood,
inducing astringency, warming the inner body, clearing the water and clearing dampness accordingly. At
the same time, the antients took the drug compatibility , two-ways adjustment etc seriously.
A-083
Rharmacodynamic Research on Liver Protecting, Choleretic and Immune Regulating
Function of Deduhonghua-7 Powder
60
BaTudeligen1，
Wu Enbaoyin1 ，
Na Risu1，
Han Zhiqiang1
1Institute
of Clinical Pharmacology of Traditional Mongolian Medicine, Affiliated Hospital of Inner Mongolia University for the
Nationalities, Tongliao, 028007, China
According animal experiment, observe the protecting function, choleretic and immune regulating function
of Deduhonghua-7 powder on D-GaLN acute liver injury.Method: The Mongolian Medicine
Deduhonghua-7 powder is experimented drug of this research, set high dose group (0.830.83 g•kg-1)and
low dose group(0.5 g•kg-1). All experimented groups were given drug continues by intragastric
administration for one week. Made mice acute liver cell necrosis model by intraperitoneal injection of
D-GaLN to observe morphology changes of liver, to evaluate the chemical protecting function of
Deduhonghua-7 powder objectively; observe the mouse peritoneal macrophages phagocytosis of
chicken erythrocytes, evaluate the influences of Deduhonghua-7 powder at phagocytosis function of
morphology; use the gallbladder removing and weighting method to observe the influence of
Deduhonghua-7 powder at secretion of bile; use MTT method to observe the influences of
Deduhonghua-7 powder at mouse spleen, lymph cell proliferation induced by concanavalin A.Result: At
acute liver injury experiment, comparing with normal group, the acute liver injury group’s ALT, AST of
mice serum were increased(P<0.05), comparing with modeling group, all Deduhonghua-7 powder group
and Bifendate group were decreased (P<0.05), comparing with modeling group, Deduhonghua-7 powder
group and Bifendate group’s tissue pathological changes obviously decreased. At macrophage
phagocytosis experiment, compared with control group, all treating groups’ phagocytosis rate and
phagocytosis indexes had significant differences(P<0.05). At choleretic experiment, comparing with
control group, the all Dedhuhonghua-7 powder group’s secreting of bile were increased, had significant
differences (P<0.05). At mice spleen, lymph cell proliferation experiment, comparing with control group,
the OD value of Deduhonghua-7 powder group were increased, had significant differences (P<0.05).
Deduhonghua-7 powder has hepatoprotective and enzyme decreasing function at mice acute liver injury
induced by D-GaLN; Can increase mice bile secretion significantly; Can significantly increase the
abdominal macrophage phagocytosis function; Can improve the proliferation of lymph cell induced by
ConA.
A-084
The Clinical Study Between Syndrome Type in TCM and the Serum Levels of IL-25 and
Total IgE in Patients with Bronchial Asthma
Liu Zongwei1, Zhou Xianmei 2
1Department
of Pulmonary Medicine, Lianyungang City Hospital of Traditional Chinese Medicine, Changshu 222004, Jiangsu,
China；2Department of Pulmonary Medicine, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing 210029,
Jiangsu, China
Bronchial asthma is one of the common and frequently occurring diseases in respiratory system. In
traditional Chinese medicine (TCM), the types of bronchial asthma are diagnosed via four examination
techniques including inspection, auscultation-olfaction, inquiry, and palpation. Therefore, the aim of the
current research is to study the correlation between serum interleukin 25 (IL-25), total immunoglobulin E
(IgE) and common clinical bronchial asthma with cold syndrome, bronchial asthma with heat syndrome,
61
and deficiency syndrome of both lung and kidney, explore the material basis of TCM diagnosis of the
syndromes, and provide scientific evidences for the diagnosis of the syndromes of TCM. 60 patients
with bronchial asthma and 20 healthy volunteers were involved in the current study. The patients were
devided into 3 groups according TCM theory, cold syndrome, heat syndrome, and deficiency of both
lung and kidney groups, each group consisits of 20 subjects. Blood was collected from each subject and
serum (2 ml) was separated and stored at -80 oC until IL-25 and total IgE were measured. The IL-25
level was detected by enzyme-linked immunosorbent assay (ELISA) method and the total IgE was
determined by Chemiluminescence Method. The level of serum IL-25 of bronchial asthmatic groups
were significantly higher than that of healthy control group (32.54 ± 9.12, P<0.01). Although the level of
serum IL-25 in bronchial asthma with cold syndrome type was not statistically significant compared with
the heat syndrome type (P>0.05), the IL-25 level in bronchial asthma with cold syndrome type (59.16 ±
12.94) and heat syndrome type (63.74 ± 13.74) were significantly higher than those in the deficiency
syndrome type of both lung and kidney (44.09 ± 8.67, P<0.01). The level of serum total IgE of bronchial
asthmatic groups were significantly higher than that of healthy control group（38.31 ± 26.71, P<0.01).
The level of serum total IgE in bronchial asthma with heat syndrome type（439.12 ± 279.44）was
significantly higher than that in the cold syndrome type (256.34 ± 100.20, P<0.01). The total IgE level in
cold syndrome type was markedly higher than that in the deficiency syndrome type (122.53 ± 44.83,
P<0.01). The level of serum total IgE in bronchial asthma with heat syndrome type was significantly
higher than that in the deficiency syndrome type of both lung and kidney (P<0.01). 3. In asthmatic
groups, serum level of total IgE was positively correlated with that of IL-25（r=0.354，P<0.006）. Some
correlation was observed between serum IL-25 and total IgE levels and bronchial asthma with TCM
diagnosis of the syndromes. Serum IL-25, total IgE levels could be used as a reference for the
diagnosis of TCM types of the asthma.
A-085
Clinical Observation on Chronic Obstructive Pulmonary Disease of Acute Aggravating
Stage Treated with Compound Fuercao mixture
Shi Kehua1, Zhe Zhe1, Lv Mimi1, Xiong Bidan1, Zhao Zhi xiang1
1City
affiliated hospital of Shang Hai Traditional Chinese Medicine University, Shanghai 200071
To investigate the clinical efficacy of Compound fuercao mixture on acute exacerbation of Chronic
obstructive pulmonary disease and the impacts on Serum inflammation factors. 60 acute exacerbation
of COPD patients were chosen and divided into 2 groups randomly, 29 cases in treatment group and 31
patients in control group. All patients accepted Conventional western medicine therapy; besides, the
treatment group patients took Compound fuercao mixture the same time. The treatment course for both
of the treatment group and the control group were 14 days. The total effectiveness of the
Compound fuercao mixture group is 92.59%, while the comparison group is 78.57%, there is a statistics
difference (P < 0.05)between the two group on the total effectiveness; And the treatment group ,the
levels of serum IL-8 decreased obviously. Compared with the control group,also have a statistics
difference (P < 0.05);and the treatment group, IL-2 levels were increased after the treatment, there is a
statistics difference(P<0.05)compared with the control group; There is no differences on the decrease of
the levels of serum IFN-γ (P>0.05). Exacerbation of COPD patients, using Compound fuercao mixture
and cooperating with Conventional western medicine therapy acute , can better improve patients with
62
cough, cough up phlegm, breathing symptoms, improve the clinical effect. Its mechanism may be
related to reduce the levels of IL- 8, increase the content of IL-2, and may play the role by reducing the
inflammatory reaction of the airway. It may also take effect through the adjustment of immune function.
A-086
Effective cases analysis of Professor Wu Yin-gen in differentiating and treating
interstitial lung disease
Shi Kehua, Xiong Bidan, Yu Xiao, Wu Yingen2
1Shanghai
Municipal Hospital of Traditional Chinese Medicine Affiliated to Shanghai University of Traditional Chinese Medicine,
200071, Shanghai, China; 2Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, 200032,
Shanghai, China
This article introduced the clinical experience of Professor Wu Yin-gen who differentiated and treated
interstitial lung disease by analyzing effective cases. The important point of syndrome differentiation and
treatment is that lung collaterals is the position of the disease, obstructing of lung collaterals and
intermingled phlegm and blood stasis are the basis pathogenesis of interstitial lung disease. Deficiency,
phlegm and blood stasis are the main pathological features. And tonifying the lung collaterals is the
method of treatment. Professor Wu thinks that collaterals deficiency must be tonified, and the disease can
be treated by using therapeutic principles and methods like reducing phlegm and vein relaxing, promoting
blood circulation for removing blood stasis, warming yang and benefiting qi, replenishing vital essence to
tonify the kidney,and so on. Professor Wu makes full use of drastic,toxic and insects medicine of TCM.
A-087
Experimental Studying of Type 2 Diabetes Animal Models through Decoction of
baihuginseng-chickpea mixture
Ji Yan1, Wang Cunfen1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to 854744149@qq.com
Objective: By the statistical analysis through experimental observation and laboratory testing index to
evaluate the hypoglycemic effect of the baihuginseng-chickpea mixture in type 2 diabetes animal models.
To indicate the hypoglycemic effect by the chickpea original potions decoction. It is used to provide
theoretical basis for reasonable development. Confirming the clinical guidance function of heat treatment
of Zhang zhongjing nourish "Yin" XiaoKeBing theory. Methods: Selected 90 healthy SD male rats, which
were randomly divided into blank control group, model group, baihuginseng group, chickpea decoction
group and baihuginseng-chickpea mixture group, using a high-fat diet breeding joint injection STZ
(45mg/kg) to establish type 2 diabetes animal models. Then with baihuginseng soup, chickpea decoction
and baihuginseng-chickpea mixture to intervene in three weeks to observe the changes of rats blood
glucose and the pathological changes of rat pancreas and liver. Results: (1) one week after treatment,
the increase of glucose level belonging to the three treatment groups was inferior to model group, but this
was not statistically significant. (2) two weeks after treatment, the increase of blood glucose of
baihuginseng group, chickpea decoction group was inferior to the model group, but this was not
63
statistically significant. Compared with the model group, the blood glucose of mixture group had the value
and statistical differences (P<0.05). (3) three weeks after treatment, compared with model group, the
increase of the blood glucose of the medicine-consumption groups were differences (P<0.05), the blood
glucose of baihuginsen group and the chickpea decoction group declined lower larger; the mixture group
compared to the remaining two treatment groups declined obviously (P<0.05), but compared with normal
control group there still had partial gap (P<0.05). Conclusion: (1)Baihuginseng-chickpea mixture has the
effect that reduce blood glucose.(2) The chickpea with Baihuginseng soup in reducing blood glucose has
synergistic effect.(3) The chickpea original potions decoction has the effect to reduce blood glucose .
A-088
Preliminary study on Preparation Technology, Quality Standard and Pharmacodynamic
for Nigella glandulifera Freyn Particle
Xiao Qiong1, Jiang Lin1, Li Xiaojin1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to xjlxj@126.com
Objective: (1) To establish the preparation procedure of Nigella glandulifera Freyn Particle; (2) To
establish the quality standard of Nigella glandulifera Freyn Particle. (3) Preliminary study on
Pharmacodynamic for Nigella glandulifera Freyn. Methods: (1) Extraction procedure: Use routine and
ethanol extraction with the synthetic score as index to select the optimum ethanol extraction process by
single factor design and orthogonaldesign method. (2) Formation procedure: The significance of multiple
index analysis in the formation process. (3) Quality standard: the quality standard of particle was identified
by TLC and HPLC. (4) Pharmacodynamics: Use the animal model of Kidney YangDeficiency, An
elementary research for it. Use the maximum tolerated dose shaw it can be used safely. Result:
preparation procedure: add 16 times of 60% ethanol, reflux-extracting for 2 times 1 hours each time, get
the solid extract by drying; adding 10% LHPC, the pellet were made by 93% alcohol，
adding 0.5% of stearil
acid-Mg to press particle. Angelica in particle made qualitative differentiation with TCL, the content of rutin
was understanded with HPLC. Pharmacodynamic: It was assayed that growth of sex organs,
weight-bearing swimming time of the experiment. the effects of Nigella glandulifera Freyn on mice with
kidney-yang deficiency. Acute toxicity test in mice Nigella glandulifera Freyn are not observed in animals
poisoned or mortality. Conclusions: During all the digester modeling: technique and nitial stability
study，
acute toxicity test is reliable，
the Process is controled for quality and noxious Property that will do
well data for new drug to report to a higher authority. Complex prescription decoction it can invigorate the
kidney. Acute toxicity test can be judged by its toxicity and has little or no toxicity for the further
development and application of a theoretical basis.
A-089
Preliminary Study on the Chemical Composition of Nigella glandulifera Preyn
Xu Jingjing1, Xue Jie1, Jia Xiaoguang1
64
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to Xgjia@vip.sina.com.cn
Objective: Study the chemical composition of Nigella glandulifera Preyn,establish the certain scientific
basis for future study. Methods: 1) This article study the N. glandulifera Preyn, extracted and separated
it, Determining Structures with chemical and spectral methods. 2) Determination of rutin in Nigella
glandulifera Freyn by HPLC. 3) extrated total alkaloid of this plant,Using Box-Behnke central composite
design and Minitab analyse the results. Results:1) the materia medica was extracted with chloroform, It
has
separated
11
componds
from
extraction
and
determined
5
compound.(Ⅰ
),3-O-[α-L-rhamnopyranosyl-(1-2)-α-L-arabinpyanosyl]-hederagenin(Ⅱ
),3-O-[β-D-xylopyrano
syl-(1-3)-α-L-rhamnopyranosyl-(1-2)-α-L-arabinpyanosyl]-hederagenin(Ⅲ
),3-O-[α-L-rhamnopyranosyl-(1
→2)-α-L-arabinpyranosyl-28-O-[α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranosyl-(1→6)-β-D-glucopyr
anosyl]hederagenin(Ⅳ
)and3-O-[β-D-glucopyranosyl-(1→6)-α-L-rhamnopyranosyl-(1→2)-α-L-arabinpyra
nosyl
]-28-O-[α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl]hederagenin(Ⅴ
). 2)
rutin was linear over the range of 0.02μg -0.1μg (r=0.9997).The average recovery was 95.3％
,with an RSD
of 1.4％
. 3) the optimun conditions were that EtOH density of 69%,the ratio of bittermelon to solvent of 10
mL·g-1,extracting temperature of 83℃and extracting time of 160min. Under such these conditions the
extraction rate of total alkaloid could be up to 3.89 mg·g-1 at one-time extraction. Conclusion: extraction
solvent and methods is appropriate of N. glandulifera Preyn studying，
it has identified 5 compounds by
silica gel, glucan methods; The method of determinating rutin by HPLC and extracting total alkaloid from
this plant is convenient, accurate and reproducible.
A-090
Kidney’s Concept of TCM and Mechanism, Method, Prescription, Drug & Application of
Kidney-invigorating
Wang Genfa1,2, Dong Jingcheng1*, Duan Xiaohong1,2
1Department of Integrated Traditional Chinese and Western Medicine of Huashan Hospital Affiliated Fudan University,
ShangHai,
China; 2Institute of Integrated Traditional Chinese and Western Medicine of Fudan University, Shanghai, China; *Corresponding
author: Tel.: +86 21 52888301, Fax: +86 21 52888265, E-mail address：
jcdong2004@hotmail.com
Kidney of TCM is very rich in connotation, which is thought as congenital nature and closely linked with
human birth, aging, disease, death and reproduction. Kidney plays a key role throughout life activities.
This paper described concept of kidney in TCM and mechanism, method, prescription, drug & application
of invigorating kidney, to improve our understanding for the kidney & the thought of treating different
diseases with the same method by invigorating kidney, and improve the research of kidney essence on a
new level in future.
A-091
Is “abnormal black bile” merely imaginary philosophical concept that has no medical
meaning?
65
Halmurat Upur1, HR Tang2, Batur Mamtimin 1, Peierdun Mijiti 1*, Aynur Matsidik 1, Nurmuhammat Amat 1,
Wang Duolao 3, Moore Nicholas4, Begaud Bernard4,Igor Gogol 5, Denis Dubrovin5
1
Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi, 830011, China; 2State Key Laboratory of Magnetic
Resonance and Molecular and Atomic Physics of Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences,
Wu Han, 430071, China; 3Department of Medical Statistics, London School of Hygiene and Tropical Medicine, London
WC1E 7HT, UK; 4Department of Pharmacology, University Victor -Segalen Bordeaux 2, 33076 Bordeaux, France. 5Department of
Hospital Therapy, Pavlov's Medical University, Saint-Petersburg, 197000, Russia; *Correspondence to: Peierdun Mijiti
(perdonjan@126.com)
The humoral theory, as a fundamental theory of Hippocratic medicine, has explained disease, psychology,
habit and personality for more than 1500 years. Essentially, this theory holds that the human body was
filled with four basic substances called humors-blood, phlegm, yellow bile and black bile, and that a
natural body function depends on normal coexistence of four humors and an excess or deficit of one of
these four humors would cause disease and comprise what is termed “abnormal humors” (1). From
Hippocrates onward, the humoral theory was adopted by Greek, Roman and Islamic physicians, and
became the most commonly held view of the human body among European physicians until the advent of
modern medical research in the nineteenth century. From humorlism’s points of view, Cancers was
described by two successors of Hippocratic medicine- Galen and Avicenna as a disease caused by an
excess of “black bile” (literally “savda” in Arabic) in body which would lead to “abnormal black bile ” (2,3).
Avicenna did not only provide diagnostic criteria for “abnormal black bile” but also gave tips on treatment
of cancers which were described in detail in his book-Cannon of Medicine (3). Although four humors
theory has been discarded since 19th century, it is still practiced in some parts of the world such as
Xinjiang Uygur region of China, India, and Iran etc., and some researches even indicate patients with
“abnormal black bile” were significantly different from those with “non-abnormal black bile” (abnormal
blood, yellow bile and phlegm) in terms of gene polymorphism, pre-thrombotic state and oxidative stress
etc. (4-7). However, is “abnormal black bile” an existing phenomenon with distinct biological basis or
merely imaginary philosophical concept that has no medical meaning and hindered the development of
medical medicine for more than 1500 years? We undertook this investigation to answer this question.
In humoral theory, health depends on balance of four humors (blood, phlegm, yellow bile and black bile) in
body and an excess or deficit of any one of these humors would cause disease and comprise what is
termed “abnormal humors”. Cancer was thought to be caused by an excess of “black bile” which lead to
“abnormal black bile”. However, is “abnormal black bile” an existing phenomenon with distinct biological
basis or merely imaginary philosophical concept that has no medical meaning? After 1H nuclear magnetic
resonance spectroscopy analysis of plasma samples from cancer patients with “abnormal black bile”,
non-abnormal black bile and healthy controls, here we showed cancer patients with “abnormal black bile”
had similar metabolic changes and characteristics which were different from the other two groups. Thus
we assumed “abnormal black bile” may have some biological basis and cancers may have some other
classification and treatment methods. We selected 240 patients who had a pathologically confirmed
diagnosis of 20 different cancers and staging according to AJCC system and classified them into
“abnormal black bile” group and “non-abnormal black bile” group according to humor classification criteria
described in Cannon of Medicine (3), while 50 healthy volunteers were used as controls. 1H nuclear
magnetic resonance based metabolomics approach was used to obtain the plasma metabolic profiles of
their blood samples. Fourier-transformed 1H NMR spectra of all samples were analyzed by employing
66
partial least squares discriminant analysis (PLS-DA) by SIMCA-P 11.0 software (Umetrics, Sweden) The
scores plot for healthy controls and cancer groups are completely distinct and the spatial distribution of
“abnormal black bile” syndrome group differs from “non-abnormal black bile” syndrome group significantly
as well. Results showed, Compared with healthy controls, “abnormal black bile” group had higher
concentration levels of formic acid, unsaturated lipid, Very Low Density lipoprotein (VLDL), Low Density
lipoprotein (LDL), acetone, acetate, acetoacetate, pyruvate, ß-hydrocxybutyrate, carnitine and malonic
acid (P<0.001), and lower concentration levels of leucine, alanine, ß-glucose, α-glucose, inositol, creatine
and methionine (P<0.05). Compared with “non-abnormal black bile” group, “abnormal black bile group”
had higher concentration levels of inositol, lactic acid, choline, methionine, acetone, acetoacetate, and
ß-hydrocxybutyrate (P<0.05), but lower concentration levels of alanine and α-glucose (P<0.05). These
results may suggest cancer patients with abnormal black bile and with non-abnormal black bile have
similar metabolic changes, but the extent of these changes is different. Concentration of acid metabolites
and levels of fat mobilization and glycolysis are higher and the damage to the immune function is greater
in “abnormal black bile” group, followed by “non-abnormal black bile” group and healthy controls.
Therefore, based on results above, it may be fair to assume “abnormal black bile” may have some
biological basis. Furthermore, we assumed, beside disease classification method used in modern
medicine, common complex diseases such as cancers may have some other classification methods and
typing possibility, and based on this classification method, there might be other treatment methods and
therapeutic drugs, which need to be confirmed in further research.
A-092
Bioactive fraction of Abnormal Savda Munziq and its antiproliferative activity on HL-60
cells
Murat Kizaibek1, M.Baranov 2, Halmurat Upur1*
1
Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China;
2Department
of Hospital Therapy, Pavlov's Medical University, Saint-Petersburg, 197000, Russia ；Correspondence should be
addressed to Halmurat@263.net
Objective: 1) Study the chemical composition of ASMq and quantify its main components; 2) Find the
in-vitro anticancer active components of ASMq; 3) Find preparation methods which suitable for
manufacturing of different extracts from ASMq; 4) Establish a HPLC method for analyzing ASMq and its
enriched active components; 5) Evaluate the in-vitro anticancer activity of single herbs of ASMq.
Methods: 1) The content of total flavonoids, expressed as rutin equivalents, was measured by using the
NaNO2-Al(NO3)3-NaOH colorimetric assay; The content of total phenolics, expressed as gallic acid
equivalents, was measured by using the Folin-Ciocalteu assay; The content of total saccharides,
expressed as glucose equivalents, was measured by the phenol-sulfuric acid method; The content of total
saponins, expressed as ammonium glycyrrhizinate equivalents, was measured by the vanillin–perchloric
acid method; The content of total alkaloids was measured by gravimetry. 2) Four extracts, namely Extr-1,
Extr-2, Extr-3 and Extr-4, were prepared from ASMq. Among these extracts, Extr-1､Extr-2 and Extr-3 were
obtained by macroporous resin chromatography as well as Extr-4 by polyamide chromatography;
Thin-layer chromatography (TLC) analysis and preliminary chemical tests were performed on each
extract of ASMq and the content of main components of each extract was determined; the in-vitro
antiproliferative activity of these extracts on HL-60 cells were compared; the IC50 value was determined
for Extr-4; Thin-layer chromatography (TLC) analyses of ASMq extracts were performed by using fast
blue salt B reagent (FBS) and anisaldehyde/ sulphuric acid reagent (AS) as spray reagents, respectively.
67
Silicagel 60 F254 plates were used as stationary phase. 3) Fractionation of Extr-4 was performed using
solid phase extraction (SPE). The cell viability was determined by trypan blue exclusion method after 24h,
48h and 72h of incubation (37°C, 5% CO2). Assay was repeated in two succesive trials, each of which
was performed in triplicate. 4) Accelarated solvent extraction (ASE) was applied to the extraction of single
herbs in the formula of ASMq. Evaporated at 40℃under reduced pressure and dried under a stream of air
to obtain CH2Cl2 and MeOH extract of each herb. HL-60 cells were collected during the logarithmic
growth phase, plated in wells at a density of 1.0×106 cells per ml and and allowed to attach for 24 hours.
The wells were divided into 19 groups. Cells in the first group were treated with blank solvent, while cells
in the other 18 groups were treated with CH2Cl2 extract and MeOH extract of each herb, respectively.
The final concentration of an extract of each herb in HL-60 cell medium was 100 µg/ml. The cell viability
was determined by trypan blue exclusion method after 24h, 48h and 72h of incubation (37°C, 5% CO2).
Assay was performed in triplicate. TLC analyses of herbs and ASMq extracts were performed by using
fast blue salt B reagent (FBS) and natural products/ polyethylene glycol reagent (NP/PEG) as spray
reagents, respectively. Silicagel 60 F254 plates were used as stationary phase. 5) The phenolic
compounds exist in ASMq were analyzed by means of HPLC-DAD. A Hypersil BDS-C18 was used
throughout this study. The guard column was 100 RP-18. Detection was performed at 254 nm and the
absorption spectra of compounds were recorded between 190 and 450 nm. The elution solvents used
were A (H2O-HAc, pH 2.8) and B (MeCN with an aliquot of HAc). Identification of compounds was
achieved by comparing retention time values and UV spectra as well as spiking samples with reference
compounds.Results: 1) The phytochemical screening of ASMq gave positive tests for saccharides,
saponins, phenolics, flavonoids, alkaloids, coumarins and/or lactones, organic acids, amin- oacids and
negative tests for tannins, anthocyanins, anthraquinones, cardiac glycosides. The content of total
flavonoids, total phenolics, total saccharides and total alkaloids were measured. As a result, a good
linearity between concentrations and absorbances of rutin was found in the range of 0.011～
0.130 mg/ml,
LOD and LOQ were estimated as 0.06 and 0.18 μg/ml respectively, recovery ranged from 95.1% to
97.5%, and precision was evaluated as 1.34%, the content of total flavonoids in the water extract and
ethanol extract of ASMq were quantified as 16.2mg/g and 19.6mg/g, respectively; A good linearity
between concentrations and absorbances of gallic acid was found in the range of 2.2～
7.7μg /ml, LOD and
LOQ were estimated as 0.01 and 0.03 μg/ml respectively, recovery was ranged from 99.5% to 104.8%,
and precision was evaluated as 3.39%. The content of total phenolics in the water extract and ethanol
extract of ASMq were quantified as 38.28mg/g and 21.54mg/g respectively; A good linearity between
concentrations and absorbances of glucose was found in the range of 0.0023-0.0146 mg/ml, the recovery
was determined to be 97.5% (n=9), and precision was evaluated as 1.34% (n=5), and the content of total
saccharides in Abnormal Savda Munziq were quantified as 57.99±1.36% (n=3) ; A good linearity between
concentrations and absorbances of ammonium glycyrrhizinate was found in the range of
0.0115～
0.0577mg/ml, the recovery was 90.4% (n=5), and the precision was evaluated as 1.14% (n=5).
The content of total saponins in Abnormal Savda Munziq were determined to be 4.69±0.08% (n=3) ; The
content of total alkaloids in ASMq should be 0.44% (0.21%+0.23%) at the most. 2) The result of TLC
analysis and preliminary chemical assay on ASMq extracts have indicated that Extr-1, Extr-3 and Extr-4
contain saccharides, saponins and phenolics, respectively. Extr-2 was also proved to contain phenolics
and saponins, but their respective spots are not so distinct, comparing to those observed in Extr-3 and
Extr-4. The quantification tests have shown that the content of flavonoids and phenolics in Extr-4 were
33.3%±0.7% and 13.9%±0.7%, respectively, whereas the content of saccharides in Extr-1 and the
content of saponins in Extr-3 were 51.4%±0.9% and 60.7%±0.1%, respectively. The total amount of main
components of Extr-2 has remained undetermined due to their uncertainty. Extr-4 reduced the cell viability
of HL-60 cells remarkably compared with other extracts. Its IC50 value after 24 h, 48 h and 72 h cell
exposure were determined to be 105.7 μg/ml, 95.1 μg/ml and 72.3 μg/ml, respectively. 3) The result of
in-vitro anticancer test has revealed that all SPE fractions showed various degrees of antiproliferative
activity on HL-60 cells. Among them, SPE-40 was found to exhibit the strongest inhibitory effect on the
68
proliferation of HL-60 cells by inducing a time-dependent decline in cell viability. After spraying the TLC
plates with NP/PEG reagent, all SPE fractions except SPE-80 showed yellow spots. More yellow spots
were detected in SPE-40 than in other fractions. On the TLC plate of Extr-4, SPE-40 and rutin, a common
spot with same color and same Rf value was detected. 4) The extraction of single herbs of ASMq was
performed using ASE. Data generated from cell test clearly showed that CH2Cl2 extracts of all nine herbs
and MeOH extracts of five herbs, namely Glycyrrhiza uralensis, Anchusa italica, Adiantum
capillus-veneris, Euphorbia humifusa and Lavandula angustifolia significantly reduced the viability of
HL-60 cells with a time-dependent response relationship, respectively. The antiprolifera- tive activity of
CH2Cl2 extract of each herb was shown to be stronger than that of its MeOH extract. Spots belong to
phenolics were detected in Anchusa italica, Adiantum capillus- veneris, Euphorbia humifusa and
Glycyrrhiza uralensis. CH2Cl2 extract of Glycyrrhiza uralensis showed stronger spots compared with
other extracts. Besides, Extr-4 and Glycyrrhiza uralensis have shown similar phenolic spots with same
color at same position. In addition, SPE-40 and five herbs, namely Euphorbia humifusa, Adiantum
capillus-veneris, Anchusa italica, Foeniculum vulgare, Melissa officinalis, have same flavonoid spots with
same color (yellow) at same position. 5) Using HPLC-DAD, ①
caffeic acid②rutin ③
isoquercitrin
④
isorhamnetin 3-O-rutinoside ⑤
apigenin 7-O-glucoside ⑥
rosmarinic acid⑦
luteolin and ⑧
formononetin were
found in Extr-4. Among these phenolic compounds, 3 and 6 were identified in SPE-p; 1, 3, 5 and 6 were
present both in SPE-0 and SPE-20, 2 - 6, were identified in SPE-40, whereas 4, 7 and 8, were identified
only in SPE-60. Conclusion: 1) The main components of ASMq and their contents were systematically
analyzed; methods of determining total phenolics, total flavonoids, total sugars and total saccharides in
ASMq were established to evaluate its main composition. The result will provide a scientific basis for the
fractionation and quality control of ASMq. As a result, ASMq was found to contain mainly sugars,
saponins, phenolics (including flavonoids) and alkaloids. In the order of decreasing content, they can be
arranged as sugars, saponins, phenolics and alkaloids. The sum of whole amounts of total saccharides,
total saponins and total phenolics account for two thirds of whole weight of ASMq. 2) Four extracts,
namely Extr-1, Extr-2, Extr-3 and Extr-4, were prepared from ASMq. The qualification and quantification
assays have revealed that the main components of Extr-1, Extr-3 and Extr-4 were polysaccharides,
saponins and phenolics, respectively. The preparation method of each extract was found to be simple and
suitable for industrialization. In-vitro anticancer test and TLC analysis have implied a possible correlation
between phenolic compounds and their antiproliferative activity on HL-60 cells. Among the four extracts,
Extr-4 was shown to possess the strongest antiprolifertive activity on HL-60 cells. 3) Extr-4 was further
fractionated into six subfractions by using solid phase extraction. Among these fractions, SPE-40 has
shown the strongest inhibitory effect on HL-60 cells. The result of TLC analysis revealed the presence of
more flavonoids in this fraction than in any other fractions, indicating that flavonoid could be an active
component or one of the active components in ASMq. 4) HPLC-DAD analysis has revealed the presence
of caffeic acid, rutin, isoquercitrin, isorhamnetin 3-O-rutinoside, apigenin 7-O-glucoside, rosmarinic acid,
luteolin and formononetin in Extr-4. Among these phenolic compounds, rutin, isoquercitrin, isorhamnetin
3-O-rutinoside, apigenin 7-O-glucoside and rosmarinic acid were also exist in SPE-40 (the most active
SPE fraction). In conclusion, the bioassay guided fractionation of ASMq led to the isolation of the most
active fraction rich in phenolics.
A-093
Effect of Xiatara on ultrastructure, protein and ergosterol biosynthesis in dermatophytes
Deng Shuwen1, Halmurat Upur2*
1Second
Army University; 2Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;
*Correspondence should be addressed to Halmurat@263.net
69
Objective: 1) To evaluate antifungal susceptibility of Xiatare（
XTR）
against dermatophytes, we
determined antifungal susceptibility of Xiatare against dermatophytes with M38-P and yeasts with M27-P.
We also first established NCCLs M27-P and M38-P method in Xinjiang Medical University. 2) To
investigate the effect of Xiatare on ultrastructure of dermatophytes This paper observed ultrastructure of
T.r and T.m treated by Xiatare and compare to control group. The object is to find the antifungal target of
Xiatare. 3) To investigate the effect of XTR on fungal protein. To find target of Xiatare to fungus with
SDS-PAGE. 4) To investigate effect of Xiatare, a new antifungal agent on ergosterol biosynthesis in fungi.
We know from SEM, TEM and SDS-PAGE that Xiatare had the effect on fungal cell membrane and wall
and protein. We just want to study how Xiatare inhibit ergosterol biosynthesis in fungi by the method of
GC/MS. Method: 1) Fungus Strains all came from center of fungus and mycoses research in Xinjiang
Medical University (XYZ). Xiatare was given by haboqikang Pharmacy Co. as testing drug, tebinafine was
given by Novatis Pharmacy Co. as positive drug. We examinated antifungal susceptibility of Xiatare
against dermatophytes with M38-P and to yeasts with M27-P. 2) T.r 400017 and T.m 60002 are observed,
two strains come from XYZ, testing drμg is Xiatare, given by Qikang Pharmacy Co.. Positive drug is
terbinafine given by Novitis Pharmacy Co.. Fungus treated according NCCLs M38-P. There are three
groups. One is control(just fungus, no drug treated ), one group is fungus treated by XTR(concentration:
256 μg/ml、
128 μg/ml、
64 μg/ml、
32 μg/ml) and one group is fungus treated by TBF(concentration: 0.01
μg/ml、
0.005 μg/ml、
0.0025 μg/ml、
0.00125 μg/ml). 3) T.r 400017 are observed, strain come from XYZ,
testing drug is Xiatare given by Qikang Pharmacy Co. There are two groups of which one is fungus with
no treatment, and the other is fungus treated by Xiatare. dermatophytes strains were grown at 30°C in
RPI1640 medium and grown for approximately 17-14days. Protein extracted in fungi is prepared
according general method. SDS-PAGE is routine method that have been previously described. 4) T.r
400017are observed, strain come from XYZ, testing drug is Xiatare given by Qikang Phar.Saponification
and extraction of the nonsaponifiable (NSLs) of fungi were carried out after 24h of incubation with
256μg/ml concentrations of Xiatare .Erogesterol in NSLs were separated and their concentration were
determined by the method of GC/MS .Sterol extraction and analyses according to the method of Bligh and
Dyer. Nonsaponifiable sterol analyses using GC-MS. Result: 1)①
Antifungal susceptibility of Xiatare
against 171 strains dermatophytes had been showed. The MIC90 of Xiatare to T.r is 1024μg/ml, Mean
MIC is 907μg/ml; The MIC90 of Xiatare against T.m is 1024μg/ml, Mean MIC is 1000μg/ml; The MIC90 of
Xiatare against T.vio is 1024μg/ml, Mean MIC is 1047μg/ml; The MIC90 of Xiatare against T.ton is
1024μg/ml, Mean MIC is 1023μg/ml; The MIC90 of Xiatare against M.g is 2048μg/ml, Mean MIC is
1862μg/ml; The MIC90 of Xiatare against M.canis is 2048μg/ml, Mean MIC is 2048μg/ml; The MIC90 of
Xiatare against E.f is 1024μg/ml, Mean MIC is 1023μg/ml. ②
Antifungal susceptibility of Terbinafine against
171 strains dermato- phytes had been showed. The MIC90 of Terbinafine against T.r is 0.01μg/ml, Mean
MIC is 0.0093μg/ml; the MIC90 of Terbinafine against T.m is 0.01μg/ml, Mean MIC is 0.01μg/ml; the
MIC90 of Terbinafine against T.vio is 0.01μg/ml, Mean MIC is 0.01μg/ml; the MIC90 of Terbinafine against
T.ton is 0.01μg/ml, Mean MIC is 0.01μg/ml; the MIC90 of Terbinafine against M.g is 0.08μg/ml, Mean MIC
is 0.08μg/ml; the MIC90 of Terbinafine against M.canis is 0.005μg/ml, Mean MIC is 0.005μg/ml; the
MIC90 of Terbinafine against E.f is 0.04μg/ml, Mean MIC is 0.0398μg/ml. ③
Antifungal susceptibility of
Xiatare against 69 strains cadidas had been showed in which the MIC90 of Xiatare against C.a is
16384μg/ml, Mean MIC is 14588μg/ml; the MIC90 of Xiatare against C.tro is 4096μg/ml, Mean MIC is
3614μg/ml; the MIC90 of Xiatare against C.gla is 16384μg/ml, Mean MIC is 15135μg/ml; the MIC90 of
Xiatare against C.kr is 16384μg/ml, Mean MIC is 13489μg/ml; the MIC90 of Xiatare against C.par is
16384μg/ml, Mean MIC is 16384μg/ml. Antifungal susceptibility of Terbinafine against 69 strains candidas
70
had been showed. The MIC90 of terbinafine against all candidas is 0.5μg/ml, Mean MIC is 0.5μg/ml.
④
Minimum fungicidal concentration (MFC)of Xiatare against 7 strains dermatophytes had been showed. 7
strains dermatophytes all growed. Minimum fungicidal concentration (MFC)of Terbinafine against 7
strains dermatophytes had been showed. 7 strains dermatophytes all didn’t grow. 2) ①
EM result of Xiatare
against T.r. Control group, SEM: hyphae and spore grow very nice. TEM: cross section of hyphae is
whole, we can see cell core in cell. Treated by Xiatare, SEM: as concentration increasing, hyphae swell,
rupture. TEM: as concentration increasing, cell membrane crimple, some cell wall was damaged,
substance in cell go out of cell. Treated by Terbinafine, SEM: as concentration increasing, hyphae swell,
dry. TEM: as concentration increasing, cell membrane crimple, shriveled. substance in cell go out of cell.
②
EM result of Xiatare to T.m. Control, SEM: hyphae and spore grow very nice. TEM: cross section of
hyphae is whole, we can see cell core in cell. Treated by Xiatare, SEM: as concentration increasing,
hyphae swell,rupture. TEM: as concentration increasing, cell membrane crimple, some cell wall was
damaged, substance in cell go out of cell. Treated by Terbinafine, SEM: as concentration increasing,
hyphae swell,dry. TEM: as concentration increasing, cell membrane crimple, shriveled. substance in cell
go out of cell. 3) From result of SDS-PAGE, to compare to control, protein strip is weaken in 45 kDa, 35
kDa, 25 kDa, t with a dose –dependent manner in fungi. 4) ①
Qualitative analysis of erogsterol. There is
peak in 40.94min in ion chart, the ion intension is 4.73e4 and also we search it in Wiey and Nist bank to
find it is the ion intension. There is peak in 40.50min in ion chart, the ion intension is 1.82e3 and also we
search it in Wiey and Nist bank to find it is erogsterol. ②
Quantitative analysis of erogsterol. Quantity of
Erogsterol of dermatophytes treated by XTR less 35.11% than control one. Conclusion: 1) XTR have
strong antifungal susceptibility to dermatophytes, MIC90 is 1024μg/ml. Antifungal table is T.rubrum
T.mentagrophytys. T.tonsurance. E.floccosum、
T.violaceum，
M.gypseum、
M.canis in turn. 1024～
2048μg/ml
is sensitive range. XTR is a drug inhibited fungus. 2) We can know from SEM and TEM that XTR can
affect on fungal cell wall and cell mambrance. 3) In the base of SDS-PAGE, XTR can inhibite protein
biosynthesis of Dermatophytes, and it is more than one protein affected by XTR. 4) We can know from
GC/MS results that after treated by XTR, Quantity of Erogsterol of dermatophytes treated by XTR less
35.11% than control one.
A-094
Establish the Alzheimer's disease carrying abnormal savda syndrome rat model and
observe the effects of formulae on it
Palidan Wushuer1, Nurmuhammat Amat2*, M.Baranov, Halmurat Upur3*
1Affiliated
China;
Traditional Medicine Hospital, Xinjiang Medical University, 830000, Urumqi, Xinjiang Uighur Autonomous Region
2Department
of Hospital Therapy, Pavlov's Medical University, Saint-Petersburg, 197000, Russia 3Traditional Uighur
Medicine institute, Xinjiang Medical University, Urumqi, Xinjiang Uighur Autonomous Region，
China; *Correspondence to:
Halmurat@263.net or nur818@hotmail.com
Objective: To Establish the Alzheimer's disease carrying abnormal savda syndrome rat model and
observe the effects of abnormal savda munziq on it. Methods: Establish abnormal savda syndrome rat
model using classical method, then injected β-Amyloid 1-40 on hippocampus to establish the Alzheimer's
disease carrying abnormal savda syndrome rat model, and the model group was given abnormal savda
munziq, after observing the biological characterization and testing the behavioral changes with Morris
water maze test and Step down test, compared with the abilities of learning and memorizing of the
71
different model groups. Result: Compared with the normal group, the daily food and water intake were
increased in abnormal savda syndrome group and the Alzheimer's disease carrying abnormal savda
syndrome group(P<0.01), the body weigh was decreased in abnormal savda syndrome group and the
Alzheimer's disease carrying abnormal savda syndrome group(P<0.01); the abilities of learning and
memorizing were weaken in the AD group、
abnormal savda syndrome group and the Alzheimer's disease
carrying abnormal savda syndrome group(P<0.05); the biological characterization, the abilities of learning
and memorizing were improved in the Alzheimer's disease carrying abnormal savda syndrome group +
abnormal savda munziq group (P<0.05). Conclusion: In this research the Alzheimer's disease carrying
abnormal savda syndrome rat model was established, abnormal savda munziq could improve biological
characterization、
the abilities of learning and memorizing of the Alzheimer's disease carrying abnormal
savda syndrome rats.
A-095
Establishment of hepatocarcinoma carrying abnormal savda animal model and it`s
morphological
Yusup Tuerxun1, Halmurat Upur1*
1Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous
Region，China;
*Correspondence should be addressed to Halmurat@263.net
Objective: To establish combining hepatocarcinoma disease and abnormal savda syndromes rats
model, and investigate the effect of abnormal savda on inducing and developing process of
hepatocirrhosis phase in this model. Methods: Under the direction of Traditional Uygur Medicine, multi
complcated induction methods were used to establish abnormal savda syndromes rats model for three
weeks, using DEN further to establish combining hepatocarcinoma and abnormal savda disease rats
model till hepatocirrhosis occurs, observe dynamically the surface of the liver and it’s pathological and
ultrastructural changes in 5th, 7th, 9th, 11th weeks respectively. Results: Both in the model control group
and combining disease and abnormal savda syndromes model group there are the pathological changing
process of hepatoxic lesion, hepatic proliferation, hepatocirrhosis, but the pathological changes of the
liver surface in the combining disease and abnormal savda syndromes model group is faster than model
control group. At the same time point, the dropsical level of liver cell, typeless hyperplasia, fake foliole
level in liver, the ultrastructural changes of decreasing hepatin and chondriosome in liver cells in
combining disease and abnormal savda syndromes model group are heavier than those in the model
control group. Conclusion: These findings indicate that in the process in which establishing abnormal
savda disease rat model serving as a base, using DEN further to establish combining hepatocarcinoma
disease and abnormal savda syndromes rats model, the abnormal savda hilit accelerates the inducing
and developing process of hepatocyte to hepatic Cellular proliferation to hepatocellular carcinoma and
this process is similar to the process of humans hepatocarcinoma. It is also stable and reliable.
A-096
Experimental Study on Caries Prevention by Aleppo Gall
72
Z.Jin 1, Halmurat Upur 2* , M.Baranov 3
1First
Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China; 2Traditional Uighur Medicine Institute, Xinjiang
Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China; 3Department of Hospital Therapy, Pavlov's
Medical University, Saint-Petersburg, 197000, Russia; *Correspondence should be addressed to Halmurat@263.net
Objective: The aim of this study is to use latest method and technique of Traditional Chinese Medicine to
study preventions of cariogenic bacteria and oral bacterial biofilm formation by using Aleppo Gall and
active compound, at the same time, the aim of this study was to evaluate the anticaries efficacy of Aleppo
Gall and active compound on dental caries formation and plaque microbiology in rats. Methods: 1) A
series of in vitro anticarious experiments, inhibition of growth, acid production, glucan generation, have
been carried out. 2) The visible light semiquantitative method has been used to measure biomass
(OD570) values so that the effect of medicine on biomass of oral bacteria biofilm has been discovered.
Idio-fluorochrome labeled mortuary bacteria and aliving bacteria; fluorescence microscope has been
used to observe morphosis and activity of treated-oral bacterial biofilm and untreated-oral bacterial
biofilm. 3) The caries rats models were established, so that to evaluate the anticaries efficacy of medicine
on dental caries formation and plaque microbiology in rats. Results: 1) Anticarious experiments,
including the inhibition effect of growth, acid production, glucan generation and initial adherence are
effective. 2) It was discovered that Aleppo gall and active components of Aleppo gall were able to inhibit
bacteria of dental biofilm, compared with negative control, it had significant (P<0.05). The effect of Aleppo
gall and active components of Aleppo gall on oral bacterial biofilm not only inhibits bacteria biofilm growth,
but also adjusts biofilm structure. 3) Streptococcus mutans level: Compared with the distilled water, and
the rest of the group to different extents in both strains decreased, only NaF and CHX group compared
with the distilled water there is significant difference (P <0.05); caries Keyes points: the experimental
group of drugs on the formation of dental caries in rats and the severity of the suppression in varying
degrees, of whichTurkish gall show on sulcal-surface and smooth-surface caries has a good effect (P
<0.05), but were lower than the NaF group; Turkish Gall + NaF group had no obvious role in the
synergistic inhibition of caries. Conclusions: The inhibition effect of growth, acid production, glucan
generation and initial adherence are effective; The effect of Aleppo gall and active components of Aleppo
gall on oral bacterial biofilm not only inhibits bacteria biofilm growth, but also adjusts biofilm structure; The
natural medicine inhibits rat oral Streptococcus mutans growth and metabolism, effectively prevent the
occurrence of dental caries.
A-097
General toxicology studies of Xi pa yi Kui Jie’an enema
Kasimjan Aximjan 1, Halmurat Upur1*
1Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur
Autonomous Region，China;
*Correspondence should be addressed to Halmurat@263.net
Objective：
Kui Jie’an enema (KJA), Uighur medicine has been used in the treatment of ulcerative colitis
(UC). However, there is very little information regarding the toxicity of KJA to give an assurance of safety
for clinical treatment. To provide safety information for KJA, we evaluated its acute toxicity in mice,
sub-chronic toxicity in rats and rectal irritation in rabbits. Methods: The single-dose toxicity of KJA was
73
examined using ICR mice. Mice were treated with KJA extract rectally at 0 and 10 g/kg body weight. After
a single administration, clinical signs, body weights, food and water consumption were observed every
day for two weeks. In the sub-chronic rectal toxicity study, KJA was administered to rats at 0, 2, 0.8, or 0.2
g/kg/day for 3 and 6 months after discontinuation lasted for a period of 4 weeks convalescence time.
Mortalities, clinical signs, body weight changes, food and water consumption, ophthalmologic findings,
urinalysis, hematological and biochemical parameters, gross findings, organ weights, and histological
examination were monitored during the study period. In the rectal irritation test, KJA was administered to
rabbits at 0.38、
0.04g/kg/day for 14 days. Mortalities, body weight changes, food and water consumption,
excretion were monitored during the study period. Results: We found no mortality and no abnormalities in
clinical signs, body weights, and necropsy findings for any of the animals in the acute studies following
rectal administration in the rat at up to 10 g/kg/day KJA. In the sub-chronic rectal toxicity study shows
that, no significant changes in general behavior and hematological and biochemical parameters, except
for Cl、
MCV、
MCHC、
TG、
CK、
GLB、
HGB、
T-BI、
TP、
ALT and AST parameters. No significant changes were
observed in organ weights, and histopathological results showed normal profile suggesting no
morphological alterations. In the Rectum mucosal stimulating degree in rabbits belong to without excitant
range, and not found any morphological alterations in colon and rectum. Conclusion: The present
findings indicated that KJA do not pose any significant acute toxicity in mice and chronic toxicity in rats
and may not rectal irritation.
A-098
Abnormal Savda Munziq and Munshil’s effect on the gene regulation, oxidative stress
induced cell damage and apoptosis
Li Lin 1, Lgor Gogol 2, Halmurat Upur 3*
1Biochemistryt
2Department
Department, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region ， China;
of Hospital Therapy, Pavlov's Medical University, Saint-Petersburg, 197000, Russia 3Traditional Uighur Medicine
Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China, *Correspondence should be
addressed to Halmurat@263.net
Objective: 1) To investigate the effects of Abnormal Savda Munziq and Munshil on the H2O2-induced
DNA damage and p53 protein expression in lymphocyte. 2) In order to investigate the influence of
Abnormal Savda Munziq and Munshil on H2O2–induced lymphocyte apoptosis and bcl-2, NF kappa B
protein expression. 3) To investigate the influence of Abnormal Savda Munziq and Munshil on the
transcription of p53, bcl-2 and Fas of Hela cells. Methods: 1) In this study, single cell gel electrophoresis
(SCGE) technique and MTT assay were used to measure the DNA damage and cytotoxicity of
lymphocyte induced by hydro-peroxide. 2) The cultivated lymphocytes were incubated with different
concentrations of H2O2, the apoptosis index (AI) was detected by Hoechest 33258 fluorescence dying
method and the electron microscopy to determine the influence of different does of Abnormal Savda
Munziq and Munshil on oxidative stress induced apoptosis. In the same experiment, Western blot was
employed to determine the expression of bcl-2 and NF-kappa B p65 proteins in different treatment
lymphocyte groups. 3) The mRNA of p53, bcl-2 and Fas of the Hela cells treated with Abnormal Savda
Munziq and Munshil were detected using the RT-PCR method. Results: 1) The results of SCGE and MTT
assay showed that the mark of DNA damage and cytotoxicity increase in a concentration dependent
manner as the H2O2 concentration elevated in the different groups. The Munziq of Abnormal Savda
display significant protective effect against DNA damage and inhibition of cytotoxicity induced by
74
800μmol/L H2O2 the value of DNA damage mark decrease in does dependent manner as does of
Abnormal Savda Munziq elevate. 2) The results show that lymphocyte group treated with 100μmol/L
H2O2 for 6hr result in the highest apoptosis index in this study. Both of Abnormal Savda Munziq and
Munshil can decrease apoptosis index induced by hydro –peroxide at high does, especially Munziq at
1000μg/ml can greatly decrease it. Abnormal Savda Munziq can inhibit. H2O2-induced lymphocyte
apoptosis while high does Munshil also. Western blot the result show, H2O2 treatment (1000mol/L,6h)
result in a high expression of NF-kappa B p65 protein, but this can be inhibited by both of Abnormal Savda
Munziq and Munshil at dose of 1000μg/ml. 3) The RT-PCR method result revealed that there were no
bcl-2 and Fas transcriptants apperence in the Munziq and Munshil treated Hela cell groups, just same as
that of control group. But p53 transcriptant is significantly increased in Munziq and Munshil treated Hela
cell groups comnpared with that of cortrol group, which appears no p53 transcriptant. Conclusion: 1) The
low does of Abnormal Savda Munshil show weak protective effect against hydro-peroxide induced DNA
damage while high does makes the damage even worse. 800μmol/L H2O2 treatment of lymphocyte
makes the p53 protein greatly elevated and this effect can be slightly inhibited by the pre-incubation with
both Abnormal Savda Munziq and Munshil. 2) The Abnormal Savda Munziq itself also show a obvious
up-regulation upon lymphocyte bcl-2 protein expression. So it indicate the Abnormal Savda Munziq can
up-regulate the lymphocyte bcl-2 protein expression. Contrast to Abnormal Savda Munziq, the Abnormal
Savda Munshil shows the opposite effect that can inhibit the lymphocyte bcl-2 protein expression. It
suggest that the Abnormal Savda Munziq’s up-regulation of bcl-2 protein may be associated with it’s
inhibition effect of lymphocyte apoptosis induced by hydro-peroxide. 3) It indiciated that this may be
related to the Munziq and Munshil’s capability to induce apoptosis of cancer cell.
A-099
Mechanisms research of Gujingmaisiha treatment for premature ejaculation (PE)
Song Guohong1, S.Nelski 2, Halmurat Upur3*
1Fifth
Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,China;
2Heidelberger
Traditional Chinese medicine Physiccal therapy Institute, Zwingenberg,Germany;3Traditional Uighur Medicine
Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China; *Correspondence should be
addressed to Halmurat@263.net
Objective: Observeing the function of the Xinjiang Uighur Medicine Gu-jing-mai-si-ha to the premature
ejaculation after treating medically, studying the parts of function mechanism of the Xinjiang Uygur
medicine gu-jing-mai-si-ha treatment premature ejaculation. Method: Pass the random method
observation Gu-jing-mai-si-ha treatment in front and back premature ejaculation sufferer the premature
ejaculation related questionnaire and intravaginal ejaculation latency time (IELT) and observe the lever of
nitric oxide and the prostaglandin F2a in blood plasma, and then compare with the controls. Result: Cure
15 diva the level of nitric oxide, prostaglandin F2a in blood plasma were significantly higher in treatment
group than that of the control group, P＜
0.001; the intravaginal ejaculation latency time (IELT), Chinese
index of sexual function for ejaculation (CIPE) the questionnaire grade point after treatment also
increases than before P＜
0.001. Before treatment the level of nitric oxide, prostaglandin F2a in blood
plasma, intravaginal ejaculation latency time (IELT), Chinese index of sexual function for ejaculation
(CIPE), have no significantly change between the two group, P＞
0.05. Conclusion: The mechanism of
Gu-jing-mai-si-ha curing premature ejaculation is possibly to strengthen the interaction of the relative
75
smooth muscle homeostasis in the urethra prostate gland department by increasing the expression of the
nitric oxide, prostaglandin F2a level in blood plasma, to improve a sufferer to be subjected to the prostate
gland urethra pressure chamber effect, to postpone the ejaculation arrival of urgent feeling.
A-100
Metabonomic studies of abnormal Savda syndrome complexity diseases in traditional
Uygur Medicine
Xu Wei fang1, N.Tatiana 2, T.Aji 3,Upur Halmurat4*
1
Affiliated Traditional Chinese Medicine Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous
Region, China; 2Department of Hospital Therapy, Pavlov's Medical University, Saint-Petersburg, 197000, Russia; 3Cardiovacular
Diseases Department,Bortala People Hospital ；4Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi
830011, Xinjiang Uighur Autonomous Region , China; *Correspondence should be addressed to Halmurat@263.net
Objective: In this study, we investigated plasma of complexity diseases (neoplasm, diabetes and
bronchial asthma) patients with abnormal Savda syndrome using nuclear magnetic resonance
spectroscopy (NMR), and described the metabolic variety and characteristics of complexity diseases with
abnormal Savda syndrome. Methods: 1) 50 healthy volunteers and 223 cases of abnormal Savda
syndrome neoplasm patients which are confirmed clinically were enrolled in this study. 2) 50 healthy
volunteers and 130 cases of Type 2 diabetes enrolled in this study. 3) 40 healthy volunteers and 39 cases
of abnormal Savda syndrome patients with asthma enrolled in this study. Blood collection from each
individual was carried out in the morning before breakfast. 1H NMR spectra from each samples were
recorded on nuclear magnetic resonance spectroscopy. Fourier transformed 1H NMR spectra were
corrected for phase and baseline distortions. The integral results of 1H NMR spectra were performed with
orthogonal projection to latent structure with discriminant analysis (OPLS-DA). Results: 1) Neoplasm: In
plasma of abnormal Savda syndrome neoplasm patients a wide range of amino acids including Leucine,
Alanin, Citruline, Tyrosine, Histidine, Glycine, Methionine, Isoleucine, Valine, Acetylcysteine decreased
significantly (p<0.05)and Glycoprotein, Glutamine, Myo-inositol, Lactic acid, Choline Creatine also
decreased (p<0.05). Formic acid, Acetone, Acetic acid, Acetoacetate, Pyruvate, β-Hydrocxy butyrate,
Carnitine, Malonic acid increased compared with healthy plasma metabolites(p<0.05). 2) Diabetes: The
patients of Type 2 diabetes with abnormal Savda syndrome had high concentration of glucose, very low
density lipoprotein, unsaturated lipid and acetone in blood compared with healthy controls (p<0.05), but
low concentration of isoleucine, leucine, valine, alanine, glutamate, histidine, 1-methyl histidine, tyrosine,
phenylalanine, glycine, citric acid, lactate, scyllo-inositol, creatine, creatinine and glycoprotein (p<0.05).
3) Asthma: In plasma of abnormal Savda syndrome patients with asthma the expression of isoleucine ,
leucine , valine , lactate , alanine , glycoprotein , citrate , creatine , creatinine , 1-methy histidine ,
scyllo-inositol see a significant decrease comparing with healthy group (p<0.05) , whereas , the
expression of acetone , acetoacetate , carnitine significant elevated (p<0.05). Conclusion: The patients
who had Abnormal Savda syndrome complexity diseases by the theory of Traditional Uygur Medicine like
neoplasm, diabetes and bronchial asthma had same metabolic change as they are “different disease
same syndrome” . In this study, abnormal Savda patients had similar metabolic change compared to
healthy controls. Abnormal Savda syndrome patients with lipid metabolism-related metabolites increased
as well as abnormal amino acid metabolism, cytoplasm membrane defected, oxidative damage will
occurred by abnormal antioxidation ability. These findings indicated that when the body form the
76
abnormal Savda syndrome, it is possible to accrued the energy metabolism abnormality, low immune
function, which led to various diseases such as neoplasm, diabetes and bronchial asthma. These findings
would facilitate further understanding of abnormal savda syndrome in Traditional Uygur Medicine is a
special state of complexity diseases.
A-101
Studies on platelet parameters and hepatic function changes of COPD patients with
syndrome of kidney deficiency and stagnation of phlegm
You Na 1, Halmurat Upur2*
1Xi’an
An Traditional Chinese Medicine Hospital, 2Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi
830011, Xinjiang Uighur Autonomous Region , China; *Correspondence should be addressed to Halmurat@263.net
Objective: To investigate the distribution of COPD with syndrome of kidney deficiency and stagnation of
phlegm (KDSP) and the clinical significance of platelet parameters and hepatic function changes.
Methods: 103 patients of AECOPD belonging to Severity Ⅲ
～
Ⅳ
were divided into two groups, KDSP group
and non- KDSP group, according to the differentiation of symptoms and signs for classification of
syndrome. Five platelet parameters of COPD patients, including PLT, PDW, P-LCR, PCT and MPV were
detected by automatic cytoanalyze; six serum biochemical indicators of COPD patients, including TBIL,
GPT, ALP, TP, ALB and GLB were detected by automatic biochemistry analyzer. Results: 1. Among 103
AECOPD patients observed, there were 70 cases in KDSP group, taking a percentage of 68%; and 33
cases in non-KSPD group (including 8 cases with obstruction of the lung by phlegm, 10 cases with
pulmonary retention of phlegmopyrexia, 3 cases with mental confusion caused by phlegm, 7 cases with
syndrome of water over-flowing due to YANG-insufficiency, 5 cases with insufficiency of QI of the lung
and kidney), taking 32%. There was no statistical significance between KDSP group and non-KDSP
group about gender (P>0.05); and compared with non-KDSP group, the patients in KDSP group were
elder, the difference of which had statistical significance (P<0.05). 2. Compared with the control group, the
PLT levels of KDSP group and non-KDSP group both significantly decreased (P<0.01, P<0.05), and the
decreased level of KDSP group was more significant. The PDW levels of KDSP group and non-KDSP
group both significantly increased (P<0.01, P<0.01), and the increased level of KDSP group was more
significant. The PCT levels of KDSP group and non-KDSP group both significantly decreased (P<0.01,
P<0.05). The P-LCR level of KDSP group significantly increased (P<0.05), while the P-LCR level of
non-KDSP group also increased, but had no statistical significance (P>0.05). There was no significant
difference among the groups, the control group, KDSP group and non-KDSP group about the levels of
MPV (P>0.05). 3. Compared with the control group, the TBIL level of KDSP group significantly increased
(P<0.01), while the TBIL level of non-KDSP group also increased, but had no statistical significance
(P>0.05); the ALT levels of KDSP group and non-KDSP group both significantly increased (P<0.01,
P<0.05), while the ALT level of KDSP group increased more significantly; the ALB levels of KDSP group
and non-KDSP group both significantly decreased (P<0.01, P<0.05), while the ALB level of KDSP group
decreased more significantly. There was no statistical significance among the groups, the control group,
KDSP group and non-KDSP group about the levels of ALP, TP and GLB. Conclusion: 1. KDSP is
possibly one of the main patterns of syndrome of AECOPD belonging to Severity Ⅲ
～
Ⅳ
in Xinjiang, and the
elder people are susceptible to COPD with syndrome of KDSP.2. AECOPD patients belonging to Severity
Ⅲ
～
Ⅳ
appear platelet parameters abnormality, and AECOPD patients with syndrome of KDSP appear more
77
significantly. Thus, AECOPD patients belonging to Severity Ⅲ
～
Ⅳ
, especially AECOPD patients with
syndrome of KDSP are probably susceptible to appearing platelet aggregation; and they probably have
the tendency of supervening thromboembolic disease. 3. Dynamic observation of platelet parameters
changes of COPD patients possibly conduces to early diagnosis of COPD with thromboembolic disease.
Early intervention prevents the aggravation of COPD. 4. AECOPD patients belonging to Severity Ⅲ
～
Ⅳ
present hepatic function abnormality, and AECOPD patients with syndrome of KDSP appear more
obviously. It is suggested that active symptomatic treatment and close attention to hepatic function
changes of AECOPD patients belonging to Severity Ⅲ
～
Ⅳ
, especially AECOPD patients with syndrome of
KDSP should be emphasized, in order to guiding the clinical rational administration and treatment to
AECOPD. More researches are approaching.
A-102
Studies on the Anticancer and Reversing Multidrug resistance effects of Abnormal Savda
Munziq Total Flavonoids
Maynur Eli 1, M.Baranov 2, Halmurat Upur3*
1Tumor
Center of the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous
Region,China; 2Department of Hospital Therapy, Pavlov's Medical University, Saint-Petersburg, 197000, Russia; 3Traditional
Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,China,
*Correspondence should be addressed to Halmurat@263.net
Objective: 1) To investigate the effect of Abnormal Savda Munziq Total Flavonoids on the proliferation,
apop-tosis in Caco-2. 2) In order to investigate the effects of Abnormal Savda Munziq Total Flavonoids on
DMH induced colon carcinogenesis. 3) To investigate the effect of Reversal mechanism of resistance to
5-Fu in human hepatocar-cinoma cell line BEL/Fu by abnormal Savda Munziq Total Flavonoids.
Methods: 1) The effect of ASMq-TF, on the proliferation of Caco-2 cells after exposure to different
concentration and different time was evaluated by MTT. Apoptosis rate was determined by using flow
cytometry, DNA fragmentation assay. 2) Obserb number of aberrant crypt foci (ACF) and aberrant crypt
(AC) and crypt multiplicity. 3) Sentivity to 5-Fu of BEL-/Fu cells was assessed by MTT assay. The reversal
action of ASMq-TF, IC50 value and synergic action of vincristine and ASMq-TF were determined by MTT
assay. The reversal action of ASMq-TF was also observed by clone forming text. MDR1 expression of
Pgp was examined by immunochemical assay and determination of gene expression of MDR1, MRP,
glutathione S-transferase(GSTpi) and topoisomerase Ⅱα of Bel-7402 cell line were investigated using
reverse transcription polymerase chain reaction (RT-PCR). Expression of bcl-2 protein and percentage of
cell apoptosis was detected by flow cytometry. MDR1 expression of Pgp was examined by
immunochemical assay. Results: 1) The results of MTT assay demonstrated that water soluble of
ASMq-TF ethanol extract showed different effects on Caco-2 cell grows at 48h and72h incubation. The
IC50, they were IC50 5.99mg/ml(48h)and 3.02mg/ml(72h). DNA fragmentation observed by gel
electrophoresis showed that there were no significant DNA ladders in Caco-2 after treatment with different
water soluble of ASMq-TF 0.5~5.0mg/ml , and seems to have no detectable difference between control
cultures. 2) The results showed that all doses (400, 800 and 1600 mg/kg b. w.) of ASMq-TF ethanol
extract in the pre-treatment group as well as in the treatment group significantly reduced the number of
aberrant crypt foci (ACF), aberrant crypt (AC) and crypt multiplicity (P<0.05), the organ relative weights in
pre-treatment and treatment groups were regained by ASMq-TF ethanol extract administration when
78
compared to negative control group (P<0.05). 3) The reversal effect of ASMq-TF was evaluated using
Bel/Fu cell line with innate resistance in this study. The IC50 value of ASMq-TF crude extract was
2000.68μg/ml. ASMq-TF at 390μg/ml concentration was no toxic but had a toxic effect on Bel/Fucell line
with 5-FU. The cytotoxicity of 390μg/ml ASMq-TF and 5-FU to Bel/Fu cellline was more remarkable than
only with 5-FU (P<0.01). The fold reversal of ASMq-TF combining with 5-FU was 7.8.While ASMq-TF
concentrations lower than 390μg/ml had less toxic, it had no synergetic effect with 5-FU. ASMq-TF
concentrations higher than 400μg/ml were not suitable due to cytotoxicitiy to tumor cell. Conclusion: 1)
To study the acting mechanism of the fractions of ASMq-TF ethanol extract, flow cytometry was used to
detect the cell cycle distribution of Caco-2 cells after 48h treatment with each fraction, respectively. The
percentage of HepG2 cells in phase sub-G1 was most high concentration of 7.5mg/ml. 2) These findings
suggested that ASMq-TF ethanol extract had a chemoprotective effects on DMH-induced colon
carcinogenesis, by suppressing the development of preneoplastic lesions, and probably exert protection
against the initiation and promotion steps of colon carcinogenesis. 3) We concluded that the anticancer
properties of ASMq-TF was possibly mediated through multiple pathways, suggesting multiple
ingredients, rather than single component The acting ingredients in ASMq-TF that exerted the anticancer
effect may include total flavonoids which are abundant in ASMq-TF.
A-103
Study on anti-tumor mechanism of Abnormal Savda Munziq in vivo based on
transplanted S-180 and EAC tumor model
Anwar Aikemu1, Halmurat Upur2*
1Pharmaceutical
Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,China;2Traditional
Uighur Medicine Department, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,China;
*Correspondence should be addressed to Halmurat@263.net
Objective: This study aims to explore the inhibitive effect of abnormal savda munziq on two kinds of
transplanted tumor, protective effect of abnormal savda munziq on chemotherapy by reducing toxicity,
synergistic attenuation of abnormal savda munziq on fluorouracil and further identify anticancer effect, the
anti-fatigue and hypoxia resistance effect of abnormal savda munziq in vivo by hypoxia and pole climbing
experiment. Methods: 1) Establishment of transplanted S-180 tumor model：
180 mice were randomized
to 6 groups: normal control group, tumor control group, high-dose group of ASMq, middle dose group of
ASMq, low dose group of ASMq, cyclophosphamide (CY) control group. Indexes are measured in batch.
Mouse tumor inhibition rate, spleen index, thymus index and the index of liver are measured. Variation of
mouse serum TNF-α, IL-1β, IL-2, IFN-γ, NK, LPO are observed. Pathological conditions of Tumor in mice
spleen and other organs are examined by HE staining. By the MTT method, the proliferation of spleen
lymphocytes in mice and variation of serum lymphocyte subsets such as CD3+, CD4+, CD8+,
CD4+/CD8+ are identified. 2) Establishment of transplanted S-180 tumor model: 60 mice were randomly
divided into 6 groups: normal control group, tumor control group, 5-fluorouracil (5-FU) group, high dose of
5-FU ASMq group, middle dose of 5-FU ASMq group, and low dose of 5-FU ASMq group. Variations of
mouse tumor inhibition rate, indexes of thymus, spleen and liver, mouse serum SOD, MDA, GSH-PX are
observed. 3) Establishment of transplanted EAC tumor model: 180 mice were randomly divided into six
groups, normal control group, tumor control group, high-dose group of ASMq, middle dose group of
ASMq, low dose group of ASMq , cyclophosphamide (CY) control group. Observation the mouse tumor
inhibition rate, spleen index thymus index, and the index of liver, mouse TNF-α, IL-1β, IL-2 , IFN-γ, NK,
LPO content in serum and HE staining examination of tumor in mice spleen and other organs pathological
79
conditions. Determination of the proliferation of murine spleen lymphocytes by MTT method and mouse
serum lymphocyte subsets in CD3+, CD4+, CD8+, CD4+/CD8+. 4) Establishment of the transplanted
EAC tumor model: 60 mice were randomly divided into 6 groups: normal control group, tumor control
group, 5-fluorouracil (5-FU) group, high dose of 5-FU ASMq group, middle dose of 5-FU ASMq group, and
low dose of 5-FU ASMq group. Variations of mouse tumor inhibition rate, index of spleen, thymus, and
liver, mouse serum SOD, MDA, GSH-PX are observed. 5) Establishment of chemotherapy drug toxicity
model: 50 mice were randomly divided into 5 groups: normal control group, tumor control
group，
AF（
amycin and 5-fluorouracil (5-Fu) group, AF+ASMq high dose group, AF+ASMq middle dose
group, and AF+ASMq low dose group. Spleen, liver and thymus index，
mouse serum ALT, AST, TP
content, SOD in mouse heart, MDA, GSH-PX content are observed and pathologic conditions of mouse
heart, liver and other viscera are examined by HE staining methods. 6) Establishment of transplanted
S-180 tumor model and EAC tumor model respectively: ASMq effect on mice serum SOD, MDA, GSH-PX
indexes, as well as on mouse tumor inhibition rate, spleen index, thymus index and liver index are
evaluated; ASMq effect on mice under normal pressure hypoxia tolerance test and polele test are
evaluated. Results: 1) Comparison between each dose group of ASMq and cyclophosphamide: The
spleen/weight ratio increased significantly in each dose group of ASMq (P＜
0.05). The inhibition rate of
each dose group of ASMq and cyclophosphamide were 49.5%、
64.26%、
43.43% and 50.88% respectively,
in which inhibitory effect was observed. But there were no significant differences between the
cyclophosphamide and ASMq high does group. Compared with tumor model group, serum IL-1β level
varied in ASMq every dose group significantly (P＜
0.05), the content of IL-1β was the highest in ASMq
middle dose group. Compared to the group of tumor model and positive control, Serum IL-2 content
increased in every dose group of ASMq significantly (P＜
0.05), and ASMq middle dose group was the
highest. The serum TNF-α content was higher in every dose group of ASMq than in the tumor model
group and in cyclophosphamide, there was significant difference (P＜
0.05), which was the highest in ASMq
middle dose group. Compared with tumor model group, serum IFN-γ content varied in every dose group
of ASMq significantly (P＜
0.05), the content of IL-1β was the highest in ASMq middle dose group.
Compared to tumor model group and cyclophosphamide, Serum IFN-γ content increases in every dose
group of ASMq, there was significant difference (P＜
0.05), Serum IFN-γ was the highesin in ASMq middle
dose group. The serum LPO content in every dose group of ASMq were all lower than tumor model group
and cyclophosphamide, there was significant difference (P＜
0.05), and serum LPO was the lowest in ASMq
middle dose group. Compared with tumor model group, the serum NK content varied significantly in every
dose group of ASMq, there was significant difference (P＜
0.05), the content of NK was the highest in ASMq
middle dose group. In ASMq high dose group, CD3+, CD8+ and CD4+ was reduced apparently, the
CD4+/CD8+ratio increased and The CD4+/CD8+ ratio was close to the normal value. In ASMq middle
dose group, CD8+ decreased, CD3+, CD4+ CD8+ and CD4+ was reduced apparently, the
CD4+/CD8+ratio increased. The CD4+/CD8+ ratio was close to the normal value. There was no
significant improvement in the ASMq low dose group. Comparison between normal control and tumor
model group, the SOD，
GSH-PX, MDA content in the liver of both ASMq and tumor groups were
significantly different. The difference was significant (P＜
0.05). The SOD，
GSH-PX content in the liver of
ASMq every dose group increased, there was significant difference (P＜
0.05), ASMq middle dose group
was the highest. The MDA content in mouse liver, of every dose group of ASMq were all lower than tumor
model group and cyclophosphamide group, there was significant difference (P＜
0.05). ASMq middle dose
group was the lowest. After examinations of mouse liver, spleen, thymus and other organs by HE staining
methods, Pathological conditions are all shown normal, necrosis and liquefaction of transplanted S-180
tumor mouse tumor was observed. 2) The inhibition rates of 5-Fu-ASMq high dose group, 5-Fu-ASMq
middle dose group, low dose group of 5-Fu +ASMq And 5-fluorouracil group were 52.26%, 66.41%,
44.87% and 48.02%. There was significant difference between normal control group and 5-Fu group in
SOD, GSH-PX content in serum(P＜
0.05), SOD, GSH-PX content decreased significantly in every dose
group of ASMq, In ASMq middle dose group, the highest content of SOD GSH-PX was close to the normal
80
value. The MDA value of which was lowest there was significantly reduced comparing with the 5-Fu
control group, the difference was significant (P＜
0.05). Compared to tumor model group with, serum SOD,
GSH-PX content in the model group, both ASMq high and low dose groups were significantly lower. The
difference was significant (P＜
0.05), in which the ASMq middle dose group maximum has been close to or
exceed the normal values. 3) Compared with the spleen/weight ratio each group of ASMq and
cyclophosphamide, ASMq every dose group significantly increased, there was significant difference
(P＜
0.05), in which ASMq middle dose group. The inhibition rate of each dose ASMq and
cyclophosphamide were 49.5%、
64.26%、
43.43% and 50.88% respectively, which inhibitory effect was
observed, but there has no difference between the groups of cyclophosphamide and ASMq. Compared
with the serum IL-1βcontent, ASMq every dose group significantly changed, there was significant
difference (P＜
0.05), the content of IL-1βwas the highest in which ASMq middle dose group Compared with
the serum IL-1βcontent between the group of tumor model and positive control, ASMq every dose group
significantly increased, there was significant difference (P＜
0.05 ), in which ASMq middle dose group was
the highest, The serum TNF-αcontent in ASMq every dose group were all higher than tumor model group
and cyclophosphamide, there was significant difference (P＜
0.05),. in which ASMq middle dose group was
the highest, Compared with the serum IFN-γ content, ASMq every dose group significantly changed,
there was significant difference (P＜
0.05), the content of IL-1βwas the highest in which ASMq middle dose
group. Compared with the serum IFN-γ content in tumor model group and cyclophosphamide, ASMq
every dose group of relative to rise, there was significant difference (P＜
0.05), in which ASMq middle dose
group was the highest, The serum LPO content in ASMq every dose group were all lower than tumor
model group and cyclophosphamide, there was significant difference (P＜
0.05), in which ASMq middle
dose group was the lowest, Compared with the serum NK content, ASMq every dose group significantly
changed, there was significant difference (P＜
0.05), the content of NK was the highest in which ASMq
middle dose group ASMq high dose group, CD3+、
CD8+ and CD4+ was reduced apparently, the
CD4+/CD8+ratio increased, and The CD4+/CD8+ ratio was close to the normal value. ASMq middle dose
group, CD8+ decreased, CD3+、
CD4+ CD8+ and CD4+ was reduced apparently, the CD4+/CD8+ratio
increased, elevated CD4+/CD8+ ratio increased, and this ratio was close to the normal value. There was
no significant improvement with the ASMq low dose group. Compared with, the SOD, GSH-PX, MDA
content in the liver of both ASMq and tumor groups were significantly difference. The difference was
significant (P＜
0.05 ).Compared with the SOD，
GSH-PX content in the liver of tumor model group, ASMq
every dose group of relative to rise, there was significant difference (P＜
0.05), in which ASMq middle dose
group was the highest, Compared with the MDA content in mouse liver, ASMq every dose group were all
lower than tumor model group and cyclophosphamide, there was significant difference (P＜
0.05), in which
ASMq middle dose group was the lowest, HE staining methods of mouse liver, spleen, thymus and other
organs Pathological conditions are all normally, transplanted S-180 tumor mouse tumor necrosis and
liquefaction. 4) In the 5-FU high-dose ASMq group, 5-FU medium-dose ASMq group, 5-FU low-dose
ASMq group and 5-FU group, the values of tumor inhibition rate were 42.41%、
58.09%, 41.58% and
50.83%, respectively, showing that tumor function had been weakened. Compared with the model group,
the thymus/body weight ratio was clearly reduced in the 5-FU medium-dose ASMq group and5-FU
low-dose ASMq group. Compared with the 5-FU group, the spleen/body weight ratio was clearly
increased in the 5-FU high-dose ASMq group, 5-FU medium-dose ASMq group, and 5-FU low-dose
ASMq group, and the difference was significant (P＜
0.05). The 5-FU ASMq dose groups had reduced
levels of SOD and GSH-PX compared with the 5-FU control group, and the difference was significant
(P＜
0.05). In the 5-FU medium-dose ASMq group, SOD and GSH-PX levels were high. With respect to
MDA values, the 5-FU medium-dose ASMq group had the lowest values and in comparison with the
model control group, the difference was significant (P＜
0.05). With respect to SOD and GSH-PX levels,
compared with the model group, the 5-FU high dose ASMq group had lower levels, and the difference was
significant (P＜
0.05). 5) Compared with the AF model group ,serum ALT, AST content, in every dose group
of ASMq significantly decreased, there was significant difference (P＜
0.05), which was the lowest in ASMq
81
middle dose group, ALT, AST content were close to the normal value. The TP value of ASMq middle dose
group is the highest, compared to the AF model control group, which had significantly increased, the
difference was significant (P＜
0.05). Compared with the normal control group, serum ALT, AST content in
abnormal savda munziq every dose group significantly decreased, there was significant difference
(P＜
0.05), TP value of which in the ASMq middle dose group was the highest. Compared with the AF model
group, SOD, GSH-PX content in the heart, significantly increased in ASMq every dose group. The
difference was significant (P＜
0.05), in which ASMq middle dose group had the highest content of SOD,
GSH-PX and which were close to the normal values. Compared with the AF model group, MDA value of
ASMq with middle doses was significantly lower; the difference was significant (P＜
0.05). Compared with
the normal control group SOD, GSH-PX content in the heart, ASMq high and low dose groups were
significantly lower. The difference was significant (P＜
0.05), in which the ASMq middle dose group
maximum has been close to or exceed the normal values. ASMq cannot only improve the spleen index,
but also reduce the serum levels of ALT, AST content and improve the mouse heart SOD, GSH-PX
content, and protect the heart and liver in preventing necrosis. 6) Experiments of transplanted tumor
S-180 mice model for climbing, hypoxia tolerance: Compared with cyclophosphamide group, ASMq high
dose group, medium dose group, low dose group showed significantly higher spleen/body weight ratio;
the differences were significant (P＜
0.05). Compared with tumor model group, the abnormal savda munziq
middle dose group and low dose group have obvious liver/body weight ratio changes, the difference was
significant (P＜
0.05). Compared with cyclophos- phamide group, in terms of the content of SOD, GSH-PX
in serum, every dose group of abnormal Savda Munziq is increased significantly, and is close to the
normal level; the difference was significant (P＜
0.05). The dose of MDA in abnormal savda munziq is the
lowest, compared with tumor model group; the difference was significant (P＜
0.05). Compared tumor
model group with normal controls, the time of climbing was decreased significantly (P＜
0.05). Compared
with cyclophosphamide, every dose group of abnormal savda munziq can improve the time of mice
climbing, particularly in abnormal savda munziq dose for which the effect is significant (P＜
0.05).
Compared with tumor model group, every dose group of abnormal savda munziq can enhance the time of
mice hypoxia tolerance, and there is significant difference (P＜
0.05). Compared with the normal control
group, every dose group of abnormal savda munziq can enhance the time of hypoxia tolerance; especially
for the dose of abnormal savda munziq the effect is most remarkable. 7) Experiments of transplanted
EAC tumor mice model for climbing, hypoxia tolerance test: Compared with cyclophosphamide group, the
abnormal savda munziq high dose group, middle dose group, and low dose group have increased
spleen/body weight ratio, the difference was significant (P＜
0.05). Compared with tumor model group, the
abnormal savda munziq middle dose group and the low dose group have obvious liver/body weight ratio
changes; the difference was significant (P＜
0.05). Compared the model group tumor with normal control
group, the climbing time was decreased significantly (P＜
0.05). Compared with cyclophosphamide group,
every dose group of abnormal savda munziq can improve the mice climbing time, particularly for the dose
of abnormal savda munziq the effect is significant (P＜
0.05). Compared the content of SOD, GSH-PX in
serum of cyclophosphamide group, every dose group of abnormal savda munziq was increased, in which
the content of SOD, GSH-PX of middle dose group is close to the normal value, and cyclophosphamide
group was increased significantly, the difference was significant (P＜
0.05). The MDA value of abnormal
savda munziq is lower than tumor model group. Compared with the normal control group, every dose of
abnormal savda munziq group can enhance hypoxia tolerance in mice; especially the middle dose
abnormal savda munziq is the best. Compared with tumor model, every dose group of abnormal savda
munziq was increased; the difference was significant (P＜
0.05). Compared with verapamil, the abnormal
savda munziq high and low dose groups were decreased, and the difference was significant (P＜
0.05).
Conclusion: 1) This study found that ASMq can improve immune system function, enhance antioxidant
enzyme activity in immune organs, and regulate the lymphocyte subsets levels and cytokines to reach the
purpose of tumor suppression. Performance of experiments in transplanted S-180 and EAC tumor models
in mice showed decreased mass volume, decreased weight, and necrosis and liquefaction in the tumor,
82
by thus the anti-tumor rate increased. 2) The ASMq has a good antagonism on the toxicity side effects of
the chemotherapy drug doxorubicin and 5-fluorouracil (AF), it can protect the liver and heart and reduce
the damage of chemotherapeutic drugs on the liver and heart. It can reduce serumin ALT, AST content
and improve the level of TP. It increases the content of the mouse model of heart tissue SOD and
GSH-PX, and reduces the level of MDA. Abnormal black Munziq can improve the content of serum SOD
and GSH-PX in 5-fluorouracil Reducing Toxicity Model and reduce the level of MDA. 3) The ASMq has a
function of anti-oxidation, hypoxia and anti-fatigue effect in vivo. Compared with the other control group,
ASMq can significantly improve the general living conditions of the tumor model mice, active state and
mouse fur luster, while weight is not significantly reduced. 4) It was found that compared to ASMq high
dose group and low dose group, in terms of suppressing the transplanted S-180 and EAC tumor, the
protective effect of toxic side effects induced by the chemotherapy drug doxorubicin and 5-fluorouracil
(AF), 5-fluorouracil efficiency attenuation, anti-oxidation, hypoxia and anti-fatigue effect in climbing the
pole and hypoxia experiment, ASMq middle dose group shows better effect.
A-104
Study on the Possible Mechanisms of Xinjiang Red Raspberry Fruit Extract in Reducing
Blood Pressure and Alleviating Oxidative Stress in SHR Rats
H.Jia 1, Upur Halmurat2*
1Public
Health Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China;2Traditional
Uighur Medicine Department, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China;
*Correspondence should be addressed to Halmurat@263.net
Objectives: To evaluate antihypertension effect of Xinjiang red raspberry fruit extract on spontaneously
hypertensive rats (SHR) and to study the possible mechanism from antioxidant perspective. Furthermore,
to provide a scientific basis for cardiovascular protective effect of red raspberry. Methods: 1) Red
raspberry fruit was extracted with 95% ethanol, defatted with petroleum ether, and partitioned with ethyl
acetate and n-butanol successively. Then the ethyl acetate extracts of raspberry (EER), n-butanol
extracts of raspberry (BER) and water section of raspberry (WSR): ①
using Folin-Ciocalteu method to
determination of total phenolic content and calculated as gallic acid equivalents (GAE); ②
using DPPH and
hydroxyl radical scavenging experiments to determination of antioxidant activity of different parts; ③
analysing the relationship between total phenols centent and antioxidant activity. 2) Thirty two
16-week-old male SHR rats were randomly divided into control group (SHR group), EERL group (EER
100 mg/kg·d), EERH group (EER 200 mg/kg·d) and captopril group (captopril 12.5 mg/kg·d). Another 8
age-matched male Wistar-Kyoto (WKY) rats were used as normotensive group. Systolic blood pressure
was measured by non-envasive tail-cuff method. After 5 weeks of treatment, blood and tissue samples
were collected for determination of concentrations of serum nitricoxide (NO), superoxidedismutase
(SOD), total anti-oxidation capability (T-AOC), malondialchehyche (MDA) and plasma endothelin (ET).
Using light microscopy and scanning electron microscopy to observe morphological changes of tissues.
The expression of eNOS mRNA and p22phox mRNA in aortas was detected by real time PCR (RT-PCR)
methods; aortic eNOS, p22phox and ET-1 protein expression was detected by immunohistochemistry.
Pearson analysis and multiple regression analysis were applied to analysis the relationship of indicators.
Results: 1) In different extracts, EER had the highest total phenols content (96.76 mgGAE/g) and WSR
had the lowest (10.56 mgGAE/g). In antioxidant activity experiment, different extracts of raspberry
showed good antioxidant activity, among them EER exhibited the highest activity, especially in
OH·scavenging and better than VC and gallic acid. There is a significant association between the
83
antioxidant potency and total phenolic content. 2) Antihypertension study showed that: ①
compared with
SHR group, EER group and captopril group were significantly lowered SHR rats blood pressure, declined
more evident in EERH group; ②
intervention groups also can increase SHR rat’s serum SOD, T-AOC and
NO levels, lower MDA and ET levels, compared with SHR group, SOD levels increased more obvious in
EERL and EERH group (P＜
0.01); T-AOC levels increase more obvious in EERH group (P＜
0.01); NO level
increased more obvious in EERL (P＜
0.01); MDA level decreased more obvious in captopril group and
EERH group (P＜
0.05), while the level of plasma ET decreased more obvious in EERH group (P＜
0.05); ③
EER also can affect serum lipids of SHR in a different degrees, compared with SHR group, TC level in
EERLgroup (P＜
0.05) and LDL-C level in EERH group (P＜
0.05) were decreased more obvious, while
HDL-C level increased more obvious in EERL group (P＜
0.05) and EERH group (P＜
0.01); ④
pathomorphology results showed that compared with WKY, under light microscope, SHR rat aortic
endothelial cells and smooth muscle cells arranged less regular, endometrial thickness increased
(P＜
0.01); under scanning electron microscope, SHR rats aortic endothelial cells lose their normal state,
arranged loose; after intervention, aortic intima surface showed more smoother than SHR group, most of
the endothelial cells arranged regular and tidy and artery intima-media thickness slightly reduced, more
better in EERL group (P＜
0.05); SHR rats left ventricular mass index increased as compared with WKY
(P＜
0.05), after intervention, left ventricular mass index in EERH group and captopril group decreased
more obvious (P＜
0.05); ⑤
pearson analysis showed that SHR rat blood pressure negatively correlated with
the serum NO, SOD and T-AOC, positively correlated with plasma ET and serum MDA level. 4) In
experiments aimed at describing molecular mechanisms of this effect, the expression levels of aortic
eNOS mRNA and protein were lower in SHR rats than that in WKY (P＜
0.05), and both mRNA and protein
expression of p22phox were higher than those in WKY (P＜
0.01), but ET-1 protein expression lower in SHR
rats than that in WKY (P＜
0.01); compared with SHR group, EER was able to significantly increase both
mRNA and protein expression of the SHR rat aort (P＜
0.01), lowered both mRNA and protein expression of
p22phox (P＜
0.01) and ET-1 protein expression decreased more in EERL group (P＜
0.05); pearson analysis
showed that SHR rat blood pressure negatively correlated with the eNOS mRNA and protein expression
and positively correlated with the aortic p22phox mRNA and protein expression and ET protein
expression; p22phox mRNA also negatively correlated with serum SOD activity and T-AOC; Multiple
regression analysis showed that p22phox mRNA, eNOS mRNA, serum SOD activity and ET were the
main factors that affect blood pressure. Conclusions: 1) The extracts of raspberry shows high
scavenging effects on the DPPH and hydroxyl radical, among them EER exhibits the highest activity and
shows significant correlation between the total phenolic contents and the antioxidant activity; 2) EER has
the function of reducing SHR rats blood pressure, serum lipids and improve the morphology of aortic and
cardiac anomalies; 3) The effect of red raspberry extract in lowering blood pressure of SHR rats may be
related to its antioxidant effect through increasing serum NO, SOD activity and T-AOC levels, and
decreasing plasma ET and MDA levels. This effect may be related to the activation of NO-NOS system by
scavenging free radicals, so as to improve vascular endothelial dysfunction and help to lower blood
pressure. The molecular mechanisms of this effect may be related the down regulating expression of
p22phox and ET-1 and increasing SOD activity and T-AOC, and up-regulating eNOS expression, which in
turn increases NO generation and improves endothelial dysfunction. The specific cell signaling
mechanisms of this effect are still unknown, and further studies are required to address these. 4) The
results of this study confirmed that polyphenols rich raspberry have potential cardiovascular protective
effects and further provide more clues to the development and utilization of Xinjiang raspberry resources
and research and development antihypertensive natural health products and medicines.
84
A-105
The Abnormal Savda Syndrome CD41, CD62p and fibrinogen clinical analysis of change
Cao Xiaobin1, Halmurat Upur2*
1
Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uighur Autonomous Region, China;2Traditional
Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China;
*Correspondence should be addressed to Halmurat@263.net
Objective：
Assessment and analysis of different age exception abnormal savda syndrome to patients with
Platelet Activation molecular α-particles membrane protein (CD62p), Integringene familyⅡb/Ⅲa Integrin
(CD41), Fibrinogen relationship. Methods：
Use of monoclonal antibodies and flow cytometry technology,
research and analysis of different age groups (elderly aged ≥60 year-old, middle age groups age 45-59
year-old age group, youth <45year-old) with the exception of abnormal savda syndrome of platelet
activating molecules CD41, CD62p and fib expression and correlation. Results：
Abnormal savda
syndrome of elderly group patients with CD62p, CD41 and fibrinogen were significantly higher than other
abnormal savda syndrome age patient groups (P<0.01); Abnormal savda syndrome in different age
groups of patients CD41, CD62p and fibrinogen were higher than other age groups of abnormal savda
syndrome CD41, CD62p and fibrinogen (P<0.05).The increase level of growth and age signifcantly
correlated (P<0.01). Conclusion：
①
The old age patients with abnormal savda syndrome was in the
majority. ②
Abnormal savda syndrome in older age-group patients with blood CD41, CD62p and Fibrinogen
more noticeable changes. ③For the elderly age-group patients of abnormal savda syndrome，
more
attention should be concerned about a variety of platelet activating factor stimulation in vivo platelet
activation, but also to early warning, prediction, prevention of abnormal savda syndrome to elderly
patients and the incidence of thrombosis. With age in patients with Abnormal Savda Syndrome in platelet
activating CD62p, CD41 and fibrinogen significantly increased platelet adhesion protein CD41, CD62p
expression and release, is active. The increase in the degree of age and abnormal growth of the gall
bladder syndrome were positively correlated. Abnormal savda mechanism and prognosis has an
important role in clinical applications and research.
A-106
The clinical report of Awrixim syrup to treat the Vasculitis patients,one case
G.Abduriyim 1, P.Ablimit 1, R.Jappar 1
1The
Uighur Medical Hospital of Turpan ,838000，
Turpan, Xinjiang Uighur Autonomous Region ，China
The Traditional Uygur Medicine herbal formula, Awrixim syrup is composed of seven herbal ingredients,.
It has long been used as a decoction in Traditional Uygur Medicines for cardiovascular disease and neuro
sysytem diseases. According to the Traditional Uygur Medicines theory Awrixim syrup was described to
treat the diseases through eliminating the Abnormal Savda Hilit ( dry and cold)，
improve blood circulation,
benefit to brain, heart and liver. Awrixim syrup is recorded in State Pharmacopoeia of People’s Republic
of China in the Uygur Medicine volume. We used the Awrixim syrup to treat vasculitis patients，
each 30ml,
three times a day for a total of three months. Awrixim syrup siginificantly improved the symptom of
patients.
85
A-107
The Study of Basic Exploration and Application of Uighur Medicine Xipayi Mouth Rinse in
Treatment of Gingivitis
Habiba1, S.Nelski 2, Upur Halmurat3*
1
First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China; 2Heidelberger Traditional Chinese medicine
Physiccal therapy Institute, Zwingenberg,Germany;3Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi
830011, Xinjiang Uighur Autonomous Region, China; *Correspondence should be addressed to Halmurat@263.net
Objective: ①
Studying the role of NF-κBp65 and COX-2 in the pathogenesis of gingivitis and the effect of
Xipayi mouth rinse on gingivitis at the mRNA level. ②
Observing the effect of Xipayi mouth rinse on different
concentrations on the activities of interleukin-6 (IL-6) from human gingival fibroblast (HGF) induced by
lipopolysaccharide (LPS) and the effect of Xipayi mouth rinse on the DNA synthesis and the changes of
cell cycle of human gingival fibroblast (HGF) inhibited by lipopolysaccharide (LPS). ③
Investigating the
effects of Xipayi mouth rinse on the control of dental plaque in cases with gingivitis. Methods: ①
Wistar rats
were randomly divided into 3 groups: normal group, gingivitis model group and Xipayi mouth rinse group,
and experimental gingivitis model was established in the last 2 groups by means of suturing the neck of
right upper second molar of rats, high –carbohydrate diet and P.gingivalis inoculation, Wistar rats in Xipayi
mouth rinse group treated with Xipayi mouth rinse while gingivitis model group treated with 0.9% sodium
chloride, the expression of NF-κBp65 and COX-2 mRNA in gingival tissue of 3 groups were examined by
RT-PCR. ②
HGF were stimulated with LPS at the concentration of 25ug/ml, radioimmunoassay (RIA) was
used to examine the effect of Xipayi mouth rinse at the concentrations of 12.5-200 ug/ml on the secretion
of IL-6 in the supernatant of the cell culture; Flow cytometry was used to examine the effect of Xipayi
mouth rinse at the concentration of 50μg/mL on the DNA synthesis and changes of HGF cell cycle.
③
Patients with gingivitis were randomly divided into therapy group and control group. The Xipayi mouth
rinse in therapy group and 0.9% sodium chloride in control group were used by patients for 7days and 5
times each day, plaque index(PLI), gingival index(GI), sulcus bleeding index(SBI) and other clinical
indexes were measured before and after treatment. Subgingival plaque sample were collected and
stained by congo red solution. A total of 200 bacteria were counted including spirochaeta, coccus,
bacillus, fusiformis and campylobacter. Compare the clinical and microorganism indexes between two
groups. Results: ①
Gingivitis model established successfully in gingivitis model group, compared with
the normal group, the mRNA expression of NF-κBp65 and COX-2 increased (P<0.05) in gingivitis model
group, while the expression of NF-κBp65 and COX-2 decreased (P<0.01) in Xipayi mouth rinse group.
The expression of NF-κBp65 had a relation to COX-2 in gingivitis model group (P<0.05). ②
IL-6 secreted by
HGF was significantly inhibited by Xipayi mouth rinse in a dose-dependent manner, the inhibitory effect of
Xipayi mouth rinse at the least concentration is approximately equal to the effect of dexamethasone; the
percentage of HGF in G1 phase during exponential growth was increased by LPS, while the percentage
of HGF in S phase was decreased as well as proliferation index (P<0.01). Xipayi mouth rinse ameliorated
this phenomenon. ③
PLI, SBI , GI and other clinical indexes in therapy group were improved significantly
(P<0.01)after seven- day washing, as well as percentage of spirochaeta, bacillus in dental plaque were
decreased and of coccus was increased (P<0.01, P<0.05, P<0.01). In addition, the effectiveness of
treatment in experimental group was significantly better than that in control group (P<0.01). Conclusion:
①
The expression of NF-κBp65 related to COX-2 may take part in the pathogenesis of gingivitis; Xipayi
86
mouth rinse can inhibit the secretion of pro-inflammatory cell factor IL-6 from HGF induced by LPS. Xipayi
mouth rinse may take role in the treatment of gingivitis in this way. ②
Xipayi mouth rinse can significantly
ameliorate the inhibitory effect of LPS on the prolifiration of HGF, thus suggesting the gingival tissue
regeneration effect of Xipayi mouth rinse in treatment and prevention of gingivitis. ③
Xipayi mouth rinse can
control dental plaque and relieve gingivitis effectively. ④
This study give us its scientific bases to be used in
clinic widely.
A-108
Study on the anti-inflammatory and anti-asthmatic mechanism of Hyssopus officinalis.L
active fractions
A.Nurahmat 1, N.Rozi 1, A.Rozi 1, A.Yusup 1
1Traditional
Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China;
*Correspondence should be addressed to abdiryim@yahoo.com
Objective: To determine the extraction process of the active site of Uighur Medicine Hyssopus
officinalis.L. To observe its anti-inflammatory and anti-asthmatic effect and its impact on asthma
inflammatory cells, factor in rats, and investigate the mechanism of anti-asthma and its material basis for
its clinical applications scientific basis. Methods: （
1）
Through mouse anti-inflammatory，
cough，
expectorant
experiment to observe the anti-inflammatory, cough expectorant effects of Hyssopus officinalis.L.
water，
ethanol extract and the ethyl acetate extract parts.（
2）
The asthmatic rats model was preparated by
OVA-sensitized and repeatedly spray；
the lung tissue pathological changes were observed by HE
staining；
Count of each group in BALF in leukocyte classification and EOS number；
the rats serum and
BALF ET-1 levels were detected by enzyme-linked immunosorbent assay（
ELISA）
；
the rat serum
ET-1，
IL-2，
IL-6，
TNF-ɑ levels were determined by radioimmunoassay.（
3）
Using the Rutin standards
establish Hyssopus officinalis.L total flavonoids determination method；
The Hyssopus officinalis.L
optimum extraction was selected with the L9（
34）
orthogonal design；
the AB-8 resin optimum purification
process for the total flavonoids of Hyssopus officinalis.L was selected with the L9（
34）
orthogonal
design；
through mouse anti-inflammatory，
cough，
expectorant experiment to observe the Hyssopus
officinalis.L total flavonoids anti-inflammatory，
cough expectorant effect；
through the rat on the basis of the
asthma model，
the lung tissue pathological changes were observed by HE staining；
count leukocyte
classification and EOS number of rats in each group in the BALF；
the TGF- β1 levels of rat lung tissue and
BALF were detected by using ELISA.（
4）
Respectively，
using steam distillation and SFE-CO2 extraction
method to extract Hyssopus officinalis.L essential oil and its GC-MS analysis；
the Hyssopus officinalis.L
essential oil supercritical optimum extraction process was determined with the L9（
34）
orthogonal
design；
through mouse anti-inflammatory，
cough，
expectorant experiment to observe the
anti-inflammatory，
cough expectorant effects of Hyssopus officinalis.L. essential oil；
the lung tissue
pathological changes were observed by HE staining；
count leukocyte classification and EOS number of
rats in each group in the BALF；
the TGF- β1 levels of rat lung tissue and BALF were detected by Using
ELISA；
Results:（
1）
Compared with model group，
Hyssopus officinalis.L. water，
ethanol extract and the ethyl
acetate extract had anti-inflammatory，
cough，
expectorant effect（
P <0.05）
and the role of the ethyl acetate
extract more significant.（
2）
Compared with model group，
Hyssopus officinalis.L.ethyl acetate dose group
rats BALFLy、
Eos、
Neu number and serum ET-1，
IL-2，
IL-6，
TNF-ɑlevels were significantly decreased，
the
difference was statistically significant（
P <0.05）
. By comparison between each dose group，
the above
indicators in BALF of serum differences were statistically significant（
P <0.05）
，
and it had dose dependent
trend. High and middle dose groups to improve lung tissue inflammation.（
3）
The maximum absorption
wavelength was 507nm，
it was using the colorimetric method. The standard curve equation for Y= 12.06x
- 0.003，
R² = 0.9999，
had good linear relationship；
The determination method stability（
RSD = 0.71%）
，
a high
accuracy（
RSD = 1.63%）
；
in accordance with the determination of methods for the determination of Hyssop
The medicinal content of total flavonoid of Hyssopus officinalis.L. was 2.68%，
The content of total
flavonoid of ethyl acetate was 46.5%；
The optimum extraction conditions were the eight volume times
ethanol（
70%）
，
extract three times per hour；
The AB-8 resin optimum separation and purification conditions
were sample concentration 15mg/mL，
70% ethanol，
flow rate 10mL/min，
and the content total flavonoid was
87
66.7%；
Compared with model group，
the total flavonoids of Hyssopus officinalis.L had
anti-inflammatory，
cough，
expectorant effect（
P <0.05）
；
Compared with model group，
Hyssopus officinalis.L.
total flavonoids dose group rats BALF Ly, Eos, Neu number and BALF, lung tissue TGF- β1 levels were
significantly decreased，
the difference was statistically significant（
P <0.05）and it had dose dependent
trend. Each dose groups were to improve lung tissue inflammation；
（
4）
The Hyssopus officinalis.L essential
oil was extracted by steam distillation and its content was 0.68%. There were detected 65 compounds by
GC-MS，
in which higher levels of linoleic acid（
13.83%）
，
palmitic acid（
13.80%）
（
+）
- pulegone（
8.31%）
，
（
+）
the
Spartak alcohol（
6.27%）
，
20 alkyl（
5.34%）
，
lemongrass acid（
4.07%）
；
The SFE optimum conditions were 20Pa
pressure，
35℃
，
flow 2L/min，
extract 1.5h，
75%extractioncarry（
ethanol: medicinal ratio = 75ml: 100g）
，
and its
content was 5.5%. There were detected 34 compounds by GC-MS in which higher levels of the γ-Valley
sterol（
31.882%）
，
21 alkyl（
19.953%）
，
linolenic acid（
16.279%）
，
hexadecane（
15.939%）
，
palmitic acid（
13.80
%）
，
higher content of linolenic acid ethyl ester（
8.766%）
；
Compared with model group，
the essential oil of
Hyssopus officinalis.L had anti-inflammatory，
cough，
expectorant effect（
P <0.05）
；
Compared with model
group，
Hyssopus officinalis.L. essential oil dose group BALF Ly、
Eos、
Neu number and BALF、
lung tissue
TGF- β1 levels were significantly decreased，
the difference was statistically significant（
P <0.05）
. and it had
dose dependent trend. Each dose groups were to improve lung tissue inflammation. Conclusion: The
Hyssopus officinalis.L water，
alcohol，
ethyl acetate extract，
total flavonoids，
essential oil all had significant
effect of anti-inflammatory，
cough，
phlegm，
and asthma. The role of Hyssopus officinalis.L ethyl acetate
extract was in relation with inhibition the level of Eos，
ET-1，
IL-2，
IL-6，
TNF-ɑ.The role of Hyssopus
officinalis.L total flavonoids and essential oil was in relation with inhibition the level of Eos，
TFG- β1.
Speculated that the total flavonoids and essential oil of Hyssopus officinalis.L were the material basis of
its anti-inflammatory and anti-asthmatic effect.
A-109
A study of the effects of the leaf extract of Cydonia Oblonga Miller on blood hemostasis,
coagulation and fibrinolysis in mice
Anwar Umar1,2, Zhou Wenting1, Ibadet Tohti1, A.Abliz 1, Bernard Bégaud 2, Nicholas Moore 1,2*
1Department
of pharmacology , Medicine, Xinjiang Medical University, Xinjiang 830011, China; 2Department of Pharmacology,
Universit´e de Bordeaux, 33076 Bordeaux, France; Correspondence should be addressed to Nicholas Moore,
nicholas.moore@pharmaco.u-bordeaux2.fr
Objective: To study the anti-thrombotic effect of Cydonia Oblonga Miller (COM) leaf extracts, which are
used for cardiovascular prevention in Traditional Uighur Medicine. Methods: The effects of three doses of
COM (20, 40, 80 mg/kg/day), aspirin (5 mg/kg/day) and gingko biloba extracts (20 mg/kg/day) in mice
were compared to untreated controls. Products were given orally for 14 days before the experiments.
Bleeding time and clotting time were measured in mice using the tail cutting method and glass slide
method, respectively. The death rates in pulmonary thrombosis induced by collagen-epinephrine,
thrombolysis in vitro and euglobulin lysis time (ELT) were also measured. Results: Low, medium and
high doses of COM leaf extracts dose-dependently prolonged the bleeding time (by 2.17, 2.78 and 3.63
times, respectively, vs. 2.58 for aspirin) and the clotting time (by 1.44, 2.47 and 2.48 times compared to
controls, vs. aspirin 1.91 times), and reduced the mortality rates in the pulmonary embolus model (by 27,
40 and 53%, respectively, compared to 47% for aspirin). All three doses significantly and
dose-dependently increased spontaneous thrombolysis (by 45, 55 and 63%, respectively, compared to
56% for aspirin) and shortened ELT to 71, 61 and 43% of controls (p<0.01), vs. 43% for aspirin.
Conclusion: the effects of COM on hemostasis, coagulation and fibrinolysis warrant further exploration.
A-110
About the exploration of Bloodletting therapy of Uighur Medicine
88
Amier Alye1, Tuersunjian Tohsun1
1Aksu
Prefecture Uighur Medicine Hospital, 843000, Xinjiang Uighur Autonomous Region, China
Bloodletting (or blood-letting) is the withdrawal of often little quantities of blood from a patient to cure or
prevent illness and disease. Bloodletting was based on an ancient system of medicine in which blood and
other bodily fluid were considered to be "humors" the proper balance of which maintained health. It was
the most common medical practice performed by Uighur Medicine doctors from antiquity up to the late
19th century, a time span of almost 2,000 years. The practice has now been abandoned for all except a
few specific conditions. It is conceivable that historically, in the absence of other treatments
for hypertension, bloodletting could sometimes have had a beneficial effect in temporarily reducing blood
pressure by a reduction in blood volume. However, since hypertension is very often asymptomatic and
thus undiagnosable without modern methods, this effect was unintentional. In the overwhelming majority
of cases, the historical use of bloodletting was harmful to patients. This article describes the history of
bloodletting therapy, the presently application situation, clinical significance, the operation method and
the matters needing attention, and shows the characteristics of the traditional treatment to improve the
therapeutic significance.
A-111
Analysis of intestinal bacterial communities in four group abnormal hilit hyperpietics and
normal hilit of Uighur nationality
Jiang Wei1, Halmurat Upur2, Dilxati Yimit1*, Ma Xiumin 1
1Department
of Pathogenic Biology, Xinjiang Medical University, Urumqi 830011, China; 2 Traditional Uighur Medicine Institute,
Xinjiang Medical University, Urumqi 830011, China; *Correspondence should be addressed to Yimit2316@163.com
Objective: The microbial diversity and differences of four abnormal hilit hyperpietics and normal hilit
group was investigated using nested PCR–denaturing gradient gel electrophoresis. Methods: The total
DNA in the fecal speciments of abnormal Sawda hilit and normal Sawda hilit healthy people, abnormal
Khan hilit and normal Khan healthy people (each of these hilit includes 20 samples), abnormal Sapra hilit
and normal Sapra hilit healthy people, abnormal Balgham hilit and normal Balgham hilit healthy people
(each of these hilit includes 10 samples), total 120 subjects aged 35 to 45 years old Uighur nationality
were used as templates. After the 16SrDNA genes (V6-V8 region) were amplified by using the universal
primers and analyzed by DGGE. Several bands in DGGE profile specifically existed in two group lanes
were excised, cloned, sequenced and searched in BLAST to find the closest relatives. Each band of
DGGE and the similarity of UPGMA cluster were analyzed by Quantity One4.4.0, and the diversity,
richness and evenness of intestinal microflora of bacterial diversity were analyzed by Microsoft Excel and
SPSS 17.0. Construct phylogenetic trees of intestinal flora by MEGA 5.05 software. Results: (1) The
results from UPGAMA cluster showed that the similarity of total intestinal tract bacteria. Abnormal Sawda
hilit hyperpietics(45%-87%)＞
abnormal Khan hilit hyperpietics (50-85%)＞
abnormal Balgham hilit
hyperpietics(42%-86%)＞
abnormal Sapra hilit hyperpietics (54-82%), normal Sawda hilit
(40%-80%)＞
normal Khan hilit (49%-79%)＞
normal Balgham hilit (43%-72%)＞
normal Sapra hilit (52%-75%).
(2) By analyzing the diversity, richness and evenness of four groups of total intestinal bacteria, abnormal
Khan hilit hyperpietics and normal Khan hilit, abnormal Balgham hilit hyperpietics and normal Balgham
hilit was statistically significant (P＜
0.05). Abnormal Sawda hilit hyperpietics respectively compared with
89
abnormal Khan hilit hyperpietics, abnormal Balgham hilit hyperpietics was statistically significant (P＜
0.05).
Compared with abnormal Sapra hilit, the richness and diversity of total intestinal bacteria was statistically
significant (P＜
0.05), but the evenness was not significant (P＞
0.05). (3) The sequences that frequency can
represent each sample were compared with sequences in the Genebank nucleotide sequence database
by using Blast software and phylogenetic trees were constructed. Conclusions:(1)There were some
differences of similarity, species and distribution diversity in intestinal microbial communities of four
abnormal hilit hyperpietics and normal hilit group.(2)The phylogenetic trees of intestinal flora diversity of
four abnormal hilit hyperpietics and normal hilit group in Uighur nationality and the phylogenetic trees
show that the existing of Firmicutes, Bacteroidetes, Verrucomicrobia, Butyrate-producing bacteriumand
and Uncultured bacterium were some factors that can be determinant for the form and development of
abnormal hilit hypertension.
A-112
Ancient Uighur drug treatment of coronary heart disease medication compatibility law
Mamatyili Rumat 1, Aniwar Talip 1, Abduwar Abulak 1, Axim Turgun 1
1Xinjiang
Uighur Autonomous Region Uighur Medicine Hospital, 830049, Xinjiang, China
Objective: Analysis of the traditional medicine laws concerning the coronary heart disease to find the
characteristics of its mechanism and treatment. Methods: Collecting, systemizing and summarizing the
effective prescriptions which is used to treat the symptoms of palpitation, cardiodynia, chest pain and
choking sensation in chest. And then we use the Microsoft Excel to build material data tables, We
classified the medicine by the data of screening function and analyse our frequency. Results: According
to the using frequency arrangement, the cardiotonic drug rowed in the former, next is abnormal body
fluids mature medicine, the cerebral tonic pill and the tonifying kidney and fighting the bowel medicine.
The medicines including bugloss, dracocephalum moldavica, sandalwood, silkworm cocoon and roseleaf
have a higher frequency. Conclusion: It has Initially formed a basic law for coronary heart disease
compatibility of formula which has given priority to cardiotonic drug and wrestled with the abnormal body
fluids mature medicine, the cerebral tonic pill, the tonifying kidney pill, the fighting bowel medicine and the
enhancing natural force medicine.
A-113
Anti-carcinogenic effects of the polyphenol of Abnormal Savda Munziq in association
with its cytotoxicity, apoptosis-inducing properties and telomerase activity in human
cervical cancer cells (SiHa)
G.Abliz 1, Ahmatjan.Mamat 2, W.Xinling 1, L.Hua 1, G.Dongmei 1, N.Amat 3*, Halmurat Upur3*
1Fifth
Department of Gynecology , Affiliated Tumor Hospital , Xinjiang Medical University, 830011, Urumqi , Xinjiang Uighur
Autonomous Region, China; 2Second Department of Gynecology , Affiliated Tumor Hospital , Xinjiang Medical University,
830011, Urumqi , Xinjiang Uighur Autonomous Region, China; 3Traditional Uighur Medicine Department, Xinjiang Medical
University, 393 Medical University Road, 830011, Urumqi , Xinjiang Uighur Autonomous Region, China; *Correspondence
should be addressed to Halmurat@263.net or nur818@hotmai.com
Objective: Abnormal Savda Munziq (ASMq) is a herbal preparation used in Traditional Uighur Medicine
90
for the treatment and prevention of diabetes, cardiovascular diseases, chronic asthma and cancer. The
polyphenol is main compounds contains in ASMq preparation responsible for the anticancer effect of
ASMq. Methods: In this study method, including Real-time quantitative Polymerase Chain Reaction
(RT-PCR) assay, MTT assay and flow cytometry were used to investigate the effect of polyphenol of
ASMqp on cell viability and the potential of the polyphenol of ASMq to induce apoptosis in human cervical
cancer cells SiHa and its effects on telomerase activity were investigated. Cellular morphological change
was observed by phase contrast microscopy. Results: The MTT cell viability data revealed that treatment
with polyphenol at 75~175μg/ml significantly inhibited the viability and proliferation of cells, and these
effects occurred in a concentration-dependent manner and time dependent manner (P<0.01). The
polyphenol can induce apoptosis of the SiHa cells, can increase the apoptosis rate in a
concentration-dependent manner and time dependent manner (P<0.01). The growth inhibition and
apoptosis induction by polyphenol treatment in SiHa cells was associated with the down-regulation of
anti-apoptotic Bcl-2 expression and telomerase (P<0.05) and Survivin expression. In addition, polyphenol
exerted a dose-dependent induction of FHIT expression. Conclusion: Together, these results suggest
that polyphenol may have anti-tumor effects in human cervical cancer through cytotoxicity,
apoptosis-inducing properties and telomerase activity.
A-114
Cognitive enhancement and neuroprotective effects of Abnormal Savda Munziq, a
formulation of Traditional Uygur Medicine, in a rat model of Alzheimer’s disease
Palidan Wushuer1, Nurmuhammat Amat2*, Halmurat Upur2*
1 Neurology
Department of Xinjiang Medical University affiliation Traditional Chinese Medicine Hospital, 116 Huanghe Road,
830000, PR China;2Traditional Uighur Medicine Institute of Xinjiang Medical University, 393 Medical University Road,
830011,PR China;* Correspondence should be addressed to
Halmurat.Upur, Nurmuhammat.Amat nur818@hotmail.com or
halmurat@263.net
Objective: Abnormal Savda Munziq（ASMq），a herbal preparation in Traditional Uygur Medicine as a
treatment for age relating diseases including Alzheimer’s disease. Therefore, the potential of this
preparation to protect against Alzheimer’s disease has been raised but less supported document is
available. Methods: To determine the effect of ASMq on cognitive function in animal model of
Alzheimer’s disease induced by Aβ1-42 in rat hippocampus. Wistar rats were orally given the ASMq at
doses of 2, 4 and 8g/kg BW via feeding needle for a period of 2 weeks after 1 week after the Aβ1-42
injected to rat hippocampus. Rats were tested for spatial memory using Morris water maze test and the
neurons was determined using histological techniques. Results: ASMq improved the latency time
(p<0.01), pass the number of effective area (p<0.01) in Morris water maze test. Moreover, the reduction
of neurons densities were also mitigated. Conclusion: These findings suggest that ASMq is a potential
cognitive enhancer and neuroprotectant against Alzheimer’s disease.
A-115
Composition of the essential oil and antioxidant activity of several extracts of Moluccella
laevis L. (Lamiaceae)
Güvenç, A1., Özek, G2., Hürkul, M.M1., Özek, T2., Başer, K.H.C3.
91
1Ankara
University, Faculty of Pharmacy, Department of Pharmaceutical Botany, 06100, Tandoğan, Ankara,
Turkey;2Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Eskişehir, 26470, Turkey; 3 Department of
Botany and Microbiology, King Saud University, College of Science, 11451 Riyadh, Saudi Arabia
Objective: Moluccella laevis is distributed in South Anatolia and it is İr.-Tur. element (Mill, 1982). The
present study was designed to define the chemical composition of the essential oil and antioxidant
activity of the several polarity extracts of M. spinosa. Methods: The essential oil obtained from
hydrodistilation of M. laevis was analyzed by gas chromatography and gas chromatography/mass
spectremetry (GC/MS), simultaneously. Results: Compounds representing 90.1% of this oil were
identified. The main componenet of the essential oil was found as cis-chrysanthenyl acetate (6.6 %),
hexahydrofarnesyl acetone (2 %) and fatty acids as a tetradecanoic acid (4.3 %), hexadecanoic acid
(45.2 %). TBA (thiobarbituric acid) test, qualitative and quantitative DPPH (2,2-diphenyl-1 picrylhydrazyl)
tests were used to evaluate the antioxidant activity of the extracts and essential oil. Total phenolic
contents of the BuOH extracts were found to 150 ± 4,83 mg/g in dry weight expressed as gallic acid
equivalents (GAE). All extracts showed a slightly antioxidant activity with the DPPH• test. The highest
antioxidant activity was observed with the water extract in the TBA test of this plant. Conclusion: This
investigation is the first report on the M. laevis.
A-116
Composition of the essential oil and antioxidant activity of several extracts of Moluccella
spinosa L. (Lamiaceae)
Güvenç, A1., Özek, G2., Hürkul, M.M1., Özek, T2., Başer, K.H.C3.
1Ankara
University, Faculty of Pharmacy, 06100, Tandoğan, Ankara, Turkey; 2 Department of Pharmacognosy, Faculty of
Pharmacy, Anadolu University, Eskişehir, 26470, Turkey;
3
Department of Botany and Microbiology, King Saud University,
College of Science, 11451 Riyadh, Saudi Arabia
Moluccella L. (Lamiaceae) of annual plants. Leaves are palmately crenate or incised. Calyx is large,
with obliquely campanulate tube, membranous or rigid; corolla is bilabiate, upper lip hooded, hairy,
lower 3-lobed. Genus grows mainly in the Mediterranean region and two species are native to Turkey.
These species M. laevis L. and M. spinosa L. Moluccella spinosa is distributed in South Anatolia and it
is rare (Mill, 1982). The present study was designed to define the chemical composition of the essential
oil and antioxidant activity of the several polarity extracts of M. spinosa. The essential oil obtained from
hydrodistilation of M. spinosa was analyzed by gas chromatography and gas chromatography/mass
spectremetry (GC/MS), simultaneously. Compounds representing 94.7% of this oil were identified.
The main componenet of the essential oil was found as hexahydrofarnesyl acetone (4%) and fatty acids
as a tetradecanoic acid (8%), hexadecanoic acid (41.3%). The phenolic contents of the samples were
determined using Folin-Ciocalteu's phenol reagent. TBA (thiobarbituric acid) test, qualitative and
quantitative DPPH (2,2-diphenyl-1 picrylhydrazyl) tests were used to evaluate the antioxidant activity of
the extracts and essential oil. All extracts showed a slightly antioxidant activity with the DPPH• test. The
antioxidant activity was not observed all extract and essential oil of plant. This investigation is the first
report on the M. spinosa.
A-117
Determination peptides compositions of Chickpea Sprouts by high performances
92
capillary electrophoresis
A.·Yili 1,2 , Ma.Q. L. 1,2，
Zhang JunPing 1,2, Gao.Y. H1, Zhao.B 1,2, D. Jun 1, H. A.·Aisa 1,2,*
1
Xinjiang Technical Institute of Physics and Chemistry, Academy of Sciences of the People's Republic of China, Urumqi,
Xinjiang, PRC, 830011, Beijing South Road 40-2, fax (+86-991) 3835679,China;
Key Laboratory of Xinjiang Indigenous
2
Medicinal Plants Resource Utilization,China
High Performances Capillary Electrophoresis (CE) is known as an efficient and rapid separation
technique with high resolution, which is frequently employed in the separation of biological macro
molecular such as proteins [1], peptides [2] and nucleic acids [3].In our continuation of research on
peptides from chickpea sprout [4], herein we report preparation of basic peptides fraction of chickpea
sprouts[5] as well as quantify the amount of peptides and determine the purity of a peptides extracts by
High Performances Capillary Electrophoresis and evaluation antioxidant activity in vitro.
CE Chromatography for Standard proteins and sample was as follow:
PDA - 220nm
Name
A
0.00
-0.01
-0.01
0
c
AU
0.01
50kD
10kD
0.00
20kD
0.01
0.02
(225kD)
150kD
0.02
AU
100kD
0.03
35kD
0.03
5
10
15
20
25
30
Minutes
0. 05
0. 05
PD A - 220n m
yz d b
Qual i ty
0. 04
B
0. 03
AU
0. 02
9803.8260
10525.0440 9960.2810
11117.2990
11707.8740
12676.6040
0. 02
0. 01
18302.7980
0. 01
31806.1250
AU
8020.9690
8330.4730
8673.6060
9372.0610
0. 03
0. 04
0. 00
0. 00
0
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
Minut es
Fig.1. CE Chromatogram was developed by PA 800 Enhanced Systems and the PA 800plus
Pharmaceutical Analysis Systems. CE Chromatogram A, Protein Size Standard, Peak identities: 10, 20,
35, 50, 100, 150, and 225 kDa proteins. CE Chromatogram B. Basic peptides fraction of Chickpea
Sprouts; Our results indicated that basic total peptides fraction of chickpea sprout was composed by at
least 7 peptides which molecular weights range from 7KDa to 12.8KDa, among them two peptides that
appeared at 9.7min and 10.25 min representative to major peptides compositions of this fraction;
Although we have isolated two individual bioactive peptides from this fraction and determined it part of the
primary structure in previous works, this result indicated that but there are others peptides still to be need
isolated for full identification peptides material basis of traditional medicine of Chickpea.
Capillary electrophoresis (CE) has become an effective replacement for manual slab gel electrophoresis
processes due to its automation, quantization, fast speed, and high efficiency. Based on this, we
developed a method for the analysis of the peptides compositions of plants extracts by High
Performances Capillary Electrophoresis. This method has the advantage of speed, sensitivity, and
reproducibility and could be applied for quality control of the medicinal plants on the basis of their peptides
93
compositions.
A-118
Effect of Cydonia Oblonga on experimental Thrombosis in rats
Zhou Wenting1, Anwar Umar1,2*, Wuliya Yimin1, A.Abliz 1, I.Tohti 1, Bégaud Bernard2, Moore Nicholas1,2*
1Department
de
of Parmacology, Xinjiang Medical University, Xinjiang 830011, China; 2Department of Pharmacology, Universit´e
Bordeaux,
33076
Bordeaux,
France;
*Correspondence
should
be
addressed
to
Nicholas
Moore,
nicholas.moore@pharmaco.u-bordeaux2.fr
Objective: Cydonia Oblonga Miller. (COM) leaf is used in traditional Uyghur medicine for prevention of
cardiovascular disease. This study is to investigate the effects of COM as an antithrombotic agent.
Methods: Two models of experimental thrombosis: common carotid artery FeCl3-induced thrombus
formation and inferior vena cava thrombosis occlusion time were used to evaluate the effects of COM on
arterial and venous thrombosis in rats. Low 20mg/kg, medium 40mg/kg, high 80mg/kg doses of COM
aqueous extracts were compared with the model group, aspirin 5mg/kg and Ginkgo 20 mg/kg. The
plasma concentrations of thromboxane B2 (TXB2) and 6-keto-prostaglandine F1 alpha (6-keto-PGF1)
were measured. Results: The venous occlusion time (OT) was prolonged and arterial and venous
thrombus weights were dose-dependently reduced in COM-treated groups compared with the model
group (p<0.01). Aspirin and gingko had effects similar to the effects of the medium dose of COM.. TXB2
concentrations were dose-dependently decreased and the concentrations of 6-keto-PGF 1 increased in
all COM-treated groups and aspirin-treated group compared with the model group (p<0.05). Conclusion:
There was a clear association between 6-keto-PGF 1/TXB2 concentration ratios and arterial or venous
thrombus weight for all products, and for occlusion time with COM but not aspirin or gingko. COM
confirmed in vivo antithrombotic effects that may be related to its traditional use in Uygur Medicine to
prevent cardiovascular disease.
A-119
Effects of Ocimum Basilicum L. extracts on Lipid Metabolism and Oxidant Status in
Hyperlipidemic rats
Anwar Umar1,2, Zhou Wenting1, Wuliya Yimin1, Guzalnur Imam 1, A.Hasan 1, I.Tohti1*, B.Berké2, Bernard
Bégaud 2, Nicholas Moore 1,2*
1Department
de
of Pharmacology，Xinjiang Medical University, Xinjiang 830011, China; 2Department of Pharmacology, Universit´e
Bordeaux,
33076
Bordeaux,
France;
Correspondence
should
be
addressed
to
Nicholas
Moore,
nicholas.moore@pharmaco.u-bordeaux2.fr
Objective: Ocimum Basilicum L (OBL) has effets on blood pressure and platelet function. To test a
possible effect on lipid metabolism, we investigated the effects of OBL in rats fed with a high-fat emulsion.
Methods: Male Wistar rats fed high-fat emulsion by gavage were treated with aqueous (A-) and ethanol
(E-) OBL extracts 100, 200 or 400 mg/kg or lovastatin 2 mg/kg, for 28 days. The animals were then tested
for serum and hepatic total cholesterol (TC), triglycerides (TG), high density lipoprotein cholesterol (HDL),
low density lipoprotein cholesterol (LDL), liver malondialdehyde (MDA), superoxide dismutase (SOD),
glutathione peroxidase (GSH-Px) and hepatic index (liver weight/body weight), and whole blood viscosity
94
and hematocrit. Results: Compared to controls, A-OBL (400mg/kg) decreased TC and LDL and
increased HDL (p<0.01); E-OBL (200 and 400mg/kg) lowered TC, TG and LDL levels and increased HDL
(p<0.01); A-OBL (200 and 400mg/kg) decreased liver TC and TG concentrations (p <0.05). All three
doses of E-OBL decreased liver TC and TG (p <0.05). The hepatic index was decreased by A-OBL
200mg/kg and by E-OBL 100mg/kg. High-fat emulsion diet increased plasma and liver MDA and
decreased SOD and GSH-PX. OBL extracts, especially E-OBL, decreased MDA and increased SOD and
GSH-Px. We also observed the improvement of morphology indexes of OBL-treated rats. Conclusion:
The aqueous and ethanol extracts of OBL may improve lipid profile and antioxidant status of rats fed with
high-fat emulsion. In addition to its other properties, OBL might be useful in the prevention and treatment
of atherosclerosis.
A-120
Effects of soybean peptide on neuroendocrine immune network, memory, hematology
and plasma biochemistry in young healthy volunteers
Nurmuhammat Amat1*, Palidan Wushuer2, Guzalnur Imam1, Dilxat Yimit 1, Nobuo Yamaguqi 3
1Traditional
Uighur Medicine Department of Xinjiang Medical University, 393 Medical University Road, 830011, PR China;
2Neurology
Department of Xinjiang Medical University affiliation Traditional Chinese Medicine Hospital, 116 Huanghe Road,
830000, PR China; 3Ishikawa Natural Medicinal Products Research Center, Fukubatake, Kanazawa, Ishikawa, Japan,
*Correspondence should be addressed to Nurmuhamamt Amat, nur818@hotmail.com
Objective: Soybean is an excellent source of dietary peptides with beneficial effects on health. We have
investigated the effect of the soybean peptides on neuroendocrine-immune network function, memory,
hematology and plasma biochemistry in young healthy volunteers. Methods: This was a 6-weeks pilot
study. A total of 18 healthy volunteers (9 male, 9 for female) years range (20-25 y) were recruited and
received soybean peptides 8 g per day for 4 weeks and neuro-endocrine immune network function,
memory , hematology, plasma biochemistry parameter were measured and analyzed at baseline(first
week and second week) and 6th week. Results: When compared with the baseline, soybean peptides
significantly increased the WBC counts and platelet counts. They were found to increase plasma IgA, IgG,
IgM concentrations but decrease IgE and TG serum concentrations as well as the ACTH, β-EP, A, NA,
DA, DOPAC level in serum. However, they increased the level 5-HA, and improved the short memory of
young healthy volunteers. Conclusion: These results suggest that treatment with soybean peptides may
have a positive effect on neuro-endocrine immune network function, memory, hematology, plasma
biochemistry. Moreover, they do not appear to have significant toxicity.
A-121
Experimental study on Vasodilation of Traditional Uygur Herb for Vitro Vascular
Lan Wei1, Jing Yuxia1, Halmurat Upur2*
1 Xinjiang
Key Laboratory of Famous Prescription and Science of Formulas, 830011, China; 2Department of Traditional Uighur
Medicine, Xinjiang Medical University, Xinjiang Uighur Autonomous Region，China; *Correspondence should be addressed to
Halmurat@263.net
95
Objective: To study on Vasodilation of Traditional Uygur Herb for Vitro vascular. Methods: Potassium
chloride (KCL) with 60mmol L-1 induce vascular to shrink, and evaluation of different varieties Uygur
Medicine (25μg•mL-1) to blood vessels of diastolic function. Results: The ethanol extraction from
Dracocephalum moldavica, Cymbopogon nardus and Cymbopogon distans (Nees) Wats have the
vasodilator effect on the constriction of Vascular induced by the high concentration KCl, and these three
extractives relaxing rate are 52.63%, 112.66% and 57.76%, then the IC50 value are 11.79μg•mL-1,
8.23μg•mL-1, 12.35μg•mL-1. The ethanol extraction from the nymphaea alba have the vasodilator rate
is 71.56%, IC50 value concentration in 16.09μg•mL-1 that have effect on the constriction of vascular
induced by the NE. Conclusion: The experimental results can offer the theory basis for development
and application of the Traditional Uygur Herb.
A-122
Retrospective analysis of hypertension and hypertensive intracerebral hemorrhage
in hospitalized patients of Uighur and Han nationnality in Hami district of Xinjiang
Aniwar 1, Abulymit 1, Wang Kun 2, Tayer 1, He Ming F2
1Hami,
Xinjiang Uighur medicine hospitals, 839000, Xinjiang Uighur Autonomous Region， China; 2First People's Hospital of
Xinxiang City, Henan Province, 453000, China
Objective: Using hospitalization samples to analyze the prevalence rates of hypertension and
hypertensive intracerebral hemorrhage between the Uighur and Han nationality hospitalized patients in
Hami of Xinjiang province. Methods: To analyze the hypertension and hypertensive intracerebral
hemorrhage we use retrospective analysis method to collect the first hospitalization clinical data of the
Uighur and Han nationality in Xinjiang Hami Center Hospital and the Region Uighur Hospital from January
1, 2005 to December 31, 2011. Results: Comparatively the prevalence rate of hypertension in the
Uighur (4.27%)and Han (2.78%)nationality showed significant difference(P=0.00，
α＜
0.05); Compared with
the prevalence rate of hypertensive intracerebral hemorrhage in the Uighur(0.99%)and
Han(0.61%)nationality, they showed significant difference(P=0.00，
α＜
0.05); the hypertensive intracerebral
hemorrhage of the Uighur and Han patients account for 23.10 and 22.04 percent of all hypertension
patients, there is no significant difference between them(P=0.50，
α＜
0.05). Conclusion: We conclude that
in Xinjiang Hami area the prevalence rates of Uighur hypertension and hypertensive intracerebral
hemorrhage is higher than that of the Han nationality. Reinforcing the prevention to Uighur patients with
cardiovascular and cerebrovascular diseases, especially at higher risk has an important clinical
significance to reduce the prevalence rates of hypertension and hypertensive intracerebral hemorrhage in
this area.
A-123
Hepatoprotective effects of Cichorium
d-galactosamine-induced Liver injury in mice
Glandulosum
leaf
extract
against
Guzalnur Imam 1, Yusupjan1, Aynur1, Nurmuhammat Amat1*
1Traditional
Uighur Medicine Institute, Xinjiang Medical University, Urumqi, Xinjiang Uighur Autonomous Region, PR China;
96
*Correspondence should be addressed to Nurmuhamamt Amat, nr818@hotmail.com
Objective: Cichorium glandulosum,a Traditional Uighur herbal medicine, has been widely used for the
treatment of chronic hepatopathy of various etiologies. Methods: The hepatoprotective activity of
Cichorium glandulosum leaf extrat was evaluated againstd-galactosamine (d-GalN)-inducedhepatic injury
inmice. GLP was administered via gavage daily for 2 weeks at doses of 100, 200 and 400mg/kg,
respectively. Control groups were given the Silymarin (25 mg/kg) synchronously. Then the mice from
d-GalN control and GLP-treated groups were treated with d-GalN (750 mg/kg) suspended in normal
saline by intraperitoneal injection. Results: d-GalN-induced hepatic damagewas manifested by a
significant increase in the activities of marker enzymes (AST, ALT) in serum and MDA level in liver (P <
0.01), and by a significant decrease in activity of SOD and GSH level in liver (P < 0.01). Pretreatment of
mice with GLP reversed these altered parameters to normal values. The biochemical results were
supplemented by histopathological examination of liver sections. The best hepatoprotective effects of
GLP were observed after treatment with the dose of 180 mg/kg as it was evidenced from biochemical
parameters and liver histopathological characters which were similar to those of normal control group.
Conclusion: Results of this study revealed that GLP could afford a significant protection in the alleviation
of d-GalN-induced hepatocellular injury.
A-124
Influence of the Uighur Medicine sand therapy in on hemorheology of the rabbit
experimental knee osteoarthritis and FEM analysis
Mahemuti D1, Halmurat Upur 2*, Kong CY1，Huang W1, Liu L1, Wei R 2
Department of Mechanical Engineering, Xin Jiang University, Urumqi,830008; 2 Traditional Uighur Medicine Department,
Xinjiang Medical University, 393 Medical University Road, Urumqi 830011, Xinjiang, PR China; * Correspondence should be
addressed to halmurat@263.net
1
Objective: The experimental and FEM analysis were used in order to test the blood flow of
osteoarthritis(OA) animal mould changed by using sand therapy. Methods: 20 New Zealand rabbit were
used to build OA animal mould, then changed them into two groups randomly, group A—the control
group, did not give any treatment, group B—sand therapy group, gave sand therapy for 20 days, tested
the parameter of the blood flow, and built the FEM analysis mould to do the transient analysis. Results:
the PS and ED P<0.01) of the blood flow increased, and RI(P<0.05) of the blood flow decreased after
sand therapy; to compare group A with group B, the PS, ED(P<0.01) of the right limbs increased.
The blood velocity increased when the inlet velocity increased and the shape of the blood vessel was
like an egg, and at the small radius of curvature the velocity of the blood was faster than the big radius
of the curvature, meanwhile, the shape of the section have different angle, it may be the reason that the
blood forward spirally. Conclusion: This kind of movement contributes to flushing the vascular wall and
reduces the formation of atherosclerosis.
A-125
Inhibitory effect of Pomegranate Blossom Flower (PGf) extract on Influenza virus
neuraminidase activity and induced Cytotoxicity
Lan Wei1，
Lan Wenjun2，
Halmurat Upur1,3*
1Xinjiang
Key Laboratory of Famous Prescription and Science of Formulas, Urumqi 830011,China; 2Institute of Biomedical
97
Engineering, Shandong Provincial Key Laboratory of Microbial Engineering, Shandong Institute of Light Industry, Jinan
250353,China; 3 Traditional Uighur Medicine Department, Xinjiang Medical University, 393 Medical University Road, Urumqi
830011, Xinjiang, PR China；Correspondence should be addressed to Halmurat@263.net
Objective: The objective of this study was to address the inhibitory effects of PGf water and ethanol
extracts on influenza A virus neuraminidase activity and induced cytotoxicity. Methods: The inhibitory
effect of PGf extract on influenza A virus (H3N2) neuraminidase activity was analyzed by an
enzyme-promoting in vitro model. The suppressing effect of PGf extract on influenza A virus-induced
MDCK cytotoxicity was assessed by crystal violet uptake method. And MNCC (maximal non-cytotoxic
concentration) of the extract was assayed by MTT method. Results: Stimulation with PGf water extract
can significantly inhibit the neuraminidase activity in a dose-dependent manner, at IC50=28.33±2.54
μg/mL. The cytotoxic response of MDCK cells to virus was observed to be concentration-dependently
suppressed by PGf water (IC50=63.04±5.26μg/mL) and ethanol extract (IC50=67.36±6.18μg/mL).
Incubated with the water extract of up to 1000 μg/mL or the ethanol extract of up to 300μg/mL, MDCK cells
survival appeared to be at least 95% of the control. Conclusions: This study suggests that PGf water
extract can inhibit influenza A virus induced cytotocity by antagonizing neuraminidase activity, and the
mechanism of PGf ethanol extract suppressing influenza A virus needs further research.
A-126
Preparation and in vitro anti-cancer activities of flavonoids from Xinjiang Glycyrrhiza
Inflata
Mourboul Ablise1, Sheng Lei2, Rena Kasim1*, C.Zhidong 3, G.Xia 2, G.Miaomiao 1, X.Fangye 1, A.Abulizi 2
1Department
of Medicinal Chemistry, College of Pharmacy; 2Xinjiang Key Laboratory of Molecular Biology and Endemic
Diseases, Xinjiang Medical University; Urumqi 830011, PR of China; 3 ITODYS-CNRS UMR 7086, University Paris 7, 75205
Paris, France; Correspondence should be addressed to renakasim@vip.sina.com
The root of Glycyrrhiza inflate Bat is an important medical herb and a main constituent of Abnormal Savda
Munziq (ASMq), a unique preparation of Traditional Uygur Medicine for commonly used in the treatment
of various cancer patients. However, the specific contribution of Glycyrrhiza inflata on anticancer activity
in ASMq prescription has not been well investigated. In this study, we investigated the in vitro anti-cancer
activity of general flavonoids (GFs) and main active constituent licochalcone A (LicoA) from Glycyrrhiza
inflata in human cervical carcinoma cells. GFs was prepared from Xinjiang Glycyrrhiza inflata and its
finger print map specter was characterized by electro-spray ionization mass spectrometry (ESI-MS);
LicoA was isolated and purified from GFs; human SiHa cervical carcinoma cells were treated with GFs
and LicoA respectively; the cell viability and cellular apoptosis were determined by MTT method and flow
cytometry. GCs and LicoA inhibited the growth and viability of cancer cells effectively with an upper limit of
87% for GFs and 97% for LicoA within the dose range of 25-100μg /ml detected by MTT. The cancel cell
apoptotic effect was 93% for GFs and 97.4% for LicoA detected by flow cytometry. The present study
demonstrated that GFs and LicoA had relatively high anti-cancer activities on human cervical carcinoma
cell line. This work may provide important evidence for further screening and developing new natural
anti-cervical cancer agents from the licorice species Glycyrrhiza inflate Bat; and this may provide also
substantial basis for explaining anticancer effect of ASMq traditional preparation.
98
(Chemical structure of licochalcone A)
References:
1. Upur, H. et al. (2011). Evidence-Based Complementary and Alternative Medicine eCAM 10,1093, 1–9.
2. Yusup, A. et al. (2007). Zhongguo Zhong Yao Za Zhi 32, 1068–1071.
3. Xie, J. et al. (2010). Chemistry of Natural Compounds 46, 148-151.
4. Yi, T.Y. et al. (2009). Journal of Agricultural Food Chemistry 57, 8266–8273.
5. Ma, S.Y. et al. (2008). Northwest Pharmaceutical Journal 23, 276–278.
6. Li, H.Z. et al. (2010). Natural Product Research and Development 22, 112–114.
7. Guo, X. et al. (2011). Natural Product Research and Development 23, 49–53.
8. Sabzevari, O. et al. (2004). Chemical-Biological Interactions 148, 57–67.
9. Kolbe, L. et al. (2006). Archives for dermatological research 298, 23.
A-127
Report of the eels and other drug treatment of facial paralysis embolism
Mamat Hujy1, Gao Jianping1
1Hami
region Uygur hospital, Hami City, Zhongshan South Road, No. 171, 839000, China
We have been using the rice field eel to treat the facial palsy, body paralysis, limb paralysis and other
diseases in our hospital from 1996. These patients daub: use the rice field eel 's blood, and at the same
time, use the soup by its meat to patients to heal for facial nerve paralysis. With this treatment to improve
the recovery efficiency. Next we will introduce this method and therapeutic effect. Introduction: there are
four normal hilit in the Uygur Medicine, Sapra hilit(SAPRA), blood hilit (KAN), phlegm hilit (BALGAM)
and Sawda hilit (SAWDA). The four hilit are distributed, and constant drain in the body, and then
continuously produce again, they keep a certain proportion and play an important role for healthy. Once
the balance were disordered, people will get sick. So in Uygur Medicine treatment, adjust the
temperament and hilit as the main treatment method.
A-128
Screening of quantitative diagnosis indices of Typing Abnormal Hilit syndrome in Uygur
Medicine using delphi method to survey: 180 cases of malignant tumor
Ayinuer Mamatisidike1, Halmurat Upur1*，Erxiding2, N.Aji 3, R.Abuwayiti 4, M.Ali 5, W.Abudulaihaiti 1, You
99
Lina 1, Y.Na 1
1 Traditional
PR China;
Uighur Medicine Department, Xinjiang Medical University, 393 Medical University Road, Urumqi 830011, Xinjiang,
2First
Affiliated Hospital of Xinjiang Medical University Oncology department Urumqi 830011, China; 3Hospital of
traditional Uighur medicine, Xinjiang Yarkan 844700; 4Hospital of traditional Uighur medicine, Xinjiang Karkax 848000; 5School of
public health, Xinjiang Medical University, Xinjiang Hutan 848000, China; Correspondence should be addressed to
Halmurat@263.net
To set up abnormal Hilit typing quantization diagnostic criteria of Uygur Medicine to the malignant tumor
patients, accordance with free disease clinical diagnostic information, 180 malignant tumor patients were
selected to the study of quantifying diagnosis specialist counseling. The selected factors were analyzed
by modified multi-categorization logistic regression analysis determined symptom-complex factors
dedication degree to the symptom-complex factors main and subsequent symptoms of abnormal Hilit of
malignant tumor were discern by OR value. As a result, the meaningful symptoms were found in all the
abnormal Hilit syndromes which is done according to the OR value. For abnormal savda type Malignant
Tumor: Chief symptoms are less eyes expression, dim lips, cyanotic tongue quality, dark and gloomy fur,
greenish urine. Subsequent symptoms are not peaceful in mind, cooler skin, thin tongue, dry stool,
sweating at night, dreaminess, nightmare. For abnormal kan type Malignant Tumor: Chief symptoms are
reddish complexion, lack of eyes expression, dark red tongue quality, hotter skin, moist and soft skin.
Subsequent symptoms are thick､strong pulse, sleep less. For abnormal balgham type Malignant Tumor:
Chief symptoms are low spirit and unpleasant, pale complexion, sticky mouth, cooler and moist skin
quality, thick and low colored urine in large amounts, much need for sleep. Subsequent symptoms are
pale and tasteless mouth, frequent and more urine .For abnormal sapra type Malignant Tumor: Chief
symptoms are easy to anger, yellowish conjunctiva, pale lips, dry mouth, dry and hotter skin quality,
thready short fast pulse. Subsequent symptoms are bittersweet taste, thin tongue, dry and yellowish
stool. As a result the feature of excess syndromes of the four abnormal Hilit are consistent with clinical
practice and the symptom quantitative diagnosis of the abnormal Hilit excess syndromes provides the
objective evidence for clinical practice.
A-129
The curative effects of 51 cases with Uighur Medicine therapy on Urticaria
Mahmutjian Ahmat1, Mamatjian Zayit1
1Aksu
Prefecture Uighur Medicine Hospital, 843000, Xinjiang China
Urticaria commonly referred to as hives, which is a kind of skin rash notable for pale red, raised, itchy
bumps. Hives are frequently caused by allergic reactions; however, there are many non-allergic causes.
Most cases of hives lasting less than six weeks (acute urticaria) are the result of an allergic trigger.
Chronic urticaria (hives lasting longer than six weeks) is rarely due to an allergy. The majority of patients
with chronic hives have an unknown (idiopathic) cause. Perhaps as many as 30–40% of patients with
chronic idiopathic urticaria will, in fact, have an autoimmune cause. Acute viral infection is an another
common cause of acute urticaria (viral exanthem).We observed the effect of Uighur medical bathe on the
urinary treatment. According to the cause, 51 cases of patients were treat with mature urticaria agent,
clearing agent after the treatment with major drug merger besmear medicine, Uighur medical bathe
treatment are effective to urticaria treatment, with the proportion reached 90%.
100
A-130
The systematic reviews of the Uighur Medical abnormal humoral typing to the treating
knee osteoarthritis
J.Xiaoyue 1, M.kelimu 1, G.Shimin 2, W.Ying 3
1The
Sixth Affiliated Hospital of Xinjiang Medical University 830002,China; 2Yunnan Institute of Traditional Chinese Medicine
650223,China; 3College of Pharmacy in Xinjiang Medical University 830019, China
Objective: To evaluate systematically the efficacy and safety of treatment osteoarthritis knee based on
abnormal humoral typing of Uighur Medicine. Methods: We searched the Chinese Biomedical Database
(CBM)、
Database(VIP)、
Chinese HowNet database(CNKI) and Wanfang Database (WanfangData) to build
a database in December 2011, which were included in the published literature on abnormal humoral
theory of Uighur Medicine clinical treatment of knee osteoarthritis. Literature screening and data
extraction were performed by two researchers independently, and then cross-checking. If there were
differences on reaching agreement the third-party’s opinions were recommended for discussion or
reference. By qualitative research methods the information and results were summarized. Results: 688
records were retrieved. The analysis results show that the Uighur medical abnormal humoral typing
theory to treat knee osteoarthritis in Xinjiang has been used for a long time and the effective rate is 84%.
Conclusion: The clinical treatment of Uighur medical abnormal humoral theory to knee osteoarthritis has
been carried out by some researches, but its quality is not very high. The demonstrated efficiency will be
improved.
A-131
Treatment of 225 cases of hair loss accordong to Uighur Medical temperament theory
analysis alopocia
Sidik Ramat, Nuermanguli Aly
1Yecheng
Uighur Medicine Hospital, 844900, Kashgar, Xinjiang, China
Objective: Observe a clinical observation to treat hair loss (alopecia areata) disease by using the Uighur
Medical temperament analysis theory. Methods: Use the Uighur Medicine which has the function of
increasing the hair internally and externally. Results: Among 255 cases , 136 cases were recovery(53.34%),
and 80 cases were markedly effective (31.37%), 29 cases were effective (11.37%), 10 cases were
invalid(3.92%), the total effective rate were 96.08%. Conclusion: To treat hair loss (alopecia areata) disease,
combined analysis of the patient's abnormal temperament, innate qualities, gender, age, history and other
factors is needed. Combined with adjustment of the mediation abnormal temperament, the internal and
external medicine therapy may enhance the curative effect.
A-132
Tradition, current situation and perspective of Traditional Uighur Medicine in China
Nurmuhammat Amat1, Palidan Wushuer 2, Aziz R A1, Halmurat Upur1*
1Traditional
Uighur Medicine Institutet of Xinjiang Medical University, 393 Medical University Road, 830011, PR China;2
101
Neurology Department of Xinjiang Medical University affiliation Traditional Chinese Medicine Hospital, 116 Huanghe Road,
830000, PR China; Correspondence should be addressed to Halmurat.Upur@263.net
Complementary and alternative medicine (CAM) is becoming more and more important in improving
human health. CAM which includes Traditional Uighur Medicine (TUM) is very popular among the
population in China and the Central Asian countries. With its history of thousands of years, TUM has
contributed to the health of the people in the West of china and Central Asia. Historical and current
surveys indicate that Xinjiang Uighur Autonomous Region of China has been distinguished throughout
the generations with a rich inventory of natural medicinal herbs. TUM, including historical background,
Current Situation and Perspective of Traditional Uighur Medicine in China, covers the medical
innovations introduced by Uighur physicians in the field of theory, herbal medicine therapies and
development of Traditional Uighur Herbal Medicine in Xinjiang Uighur Autonomous Region.
References:
1. Upur H, Yusup A (2003) Theory of Hilit and Mizaj on Uighur Modern Research on it. Science and Technology Press. Urumqi,
China.
2. Upur H, Denise D, Nurmuhammat A (2010) Uighur Medicine. ITA Rhythm.St. Petersburg, Russia.
A-133
Treating report about 190 patients with kidney stone patients treated by Uighur Medicine
Abduwayit Nurahmet1
1
Kashgar Prefecture Yecheng Uighur Medicine Hospital, The Yecheng South Jiefang Road NO.16, 844 900, china
Objective: To observe the effect of Uighur Medicine named Supopi Mupetti Hasat at treating patients
with kidney stones. Methods: From January 2005 to August 2010 we have treated 190 patients with
kidney stones, during which we identified and analyzed the relationship between the cause of kidney
stones, ages, gender and temperament of patients. And then treated them by Uighur medicine named
Supopi Mupetti Hasat and observed the effect of removing kidney stones. Results: After using the Uighur
Medicine named Supopi Mupetti Hasat on 190 patients with kidney stone we found that 117 of them
(61.6%) cured, 64 of them (33.7%) improved and 9 of them had (4.7%) no effects. It means that the
treating effect of the medicine reached 95.3%. Conclusion: Kidney stones are clinical common diseases,
and this abnormal renal function which has close relation to obstacles of Material exchange caused by
disordered Fluid balance. Kidney stone has no selection object, young and old, rich and poor, all of them
are equal to get this disease. If not treated in time, it will cause obstruction of Urinary track and infection
and it will lead to no urine, uremia, nephritis, hydronephrosis. At last it will get worse and worse and
causes Renal exhaust. Modern medicine use surgical treatment as the main treatment. Our Uighur
Medicine named Supopi Mupetti Hasat made by pure natural medicinal herbs has better effect of breaking
kidney stone and remove it, besides diuresis, analgesia, diminish inflammation, regulate humor. The
effect of the Uighur Medicine is better and faster than similar medicines, and has less adverse reactions.
A-134
A clinical report of 31 cases using Uighur Medical treatment of herpes zoster
102
Nuermangul Alya1, Sidik Ramat1, Tajigul Mamat1
1Kashgar
Yecheng Uighur Medicine Hospital Dermatology, South Jiefang Road NO.16 Yecheng , 844900,China
Objective: To observe the Uighur Medical treatment for herpes zoster clinical effect. Methods: with
anti-inflammatory, analgesic, promote skin healing inside and outside in Uighur Medicine. Results: Of the
31cases, 9 cases were cured (29%),15 cases were valid (48.4%), 6 cases were effective (19.4%)，
and
invalid in 1 case (3.2%). Conclusion: the therapy of herpes zoster disease, comprehensive analysis in
patients with disorders of fluid balance disorders, temperament, gender, age, medical history and other
factors, adjust the body fluid balance disorders and temperament disorders, with the combination of
internal and external medicine therapy, may improve the curative effect.
A-135
Uighur Medicine fusion of East-West cultural factors
Wang Xingyi1
1Shanghai
University of Traditional Chinese Medicine Institute of Chinese literature, Cai Lun Lu 1200, Pudong New Area,
Shanghai, 201203, China
Through analyzing the unearthed medical literatures from Sinkiang, catalogs of historical chronicles,
codex handed for generations and medical preservations from contemporary Uighur Medicine, which
were discovered to contain the Oriental cultures such as cultures from Central China, India, Persia, Arab
and Occidental cultures such as Hellenism, the article proved that the Traditional Uighur Medicine
integrates the cultural factors of Orient and Occident.
A-136
The effect of Abnormal Savda Munziq on Hippocampus BDNF, 5-HT1A mRNA expression
in depression animal models and Abnormal Savda Syndrome animal models
Hanzohra Upur1, Nurmuhammat Amat 1, T.Kamil 1, A.Rozi 1, Abdiryim Yusup 1*, Halmurat Upur 1*
1Traditional
Uighur Medicine Department, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;*Corresponding authors:
Abduryim Yusup，
Halmurat Upur, Traditional Uighur Medicine Department, Xinjiang Medical University, 830011 Urumqi, E-mail:
halmurat@263.net，
abdiryim@hotmai.com
This paper studies the effect of Abnormal Savda Munziq on mRNA expression of Hippocampus BDNF,
5-HT1A in depression animal models and Abnormal Savda Syndrome animal models. The mRNA
expression of Hippocampus BDNF was detected with Real-time quantitative Polymerase Chain Reaction
and mRNA expression of Hippocampus 5-HT1A was detected with RT-PCR. Compared to the control
group, mRNA expression of Hippocampus BDNF, 5-HT1A was down-regulated in depression animal
models and Abnormal Savda Syndrome animal models. The mRNA expression of Hippocampus BDNF,
5-HT1A was up-regulated in the Abnormal Savda Munziq medium-dose, high-dose groups of depression
animal models and Abnormal Savda Syndrome animal models. There was a significant difference on the
mRNA expression of Hippocampus BDNF in depression animal models and Abnormal Savda Syndrome
animal models (P<0.01). There is not a significant difference on the mRNA expression of Hippocampus
5-HT1A in depression animal models and Abnormal Savda Syndrome animal models (P>0.05). The
103
Abnormal Savda Munziq is probably related to the up-regulation of mRNA expression of Hippocampus
BDNF, 5-HT1A in depression animal models and Abnormal Savda Syndrome animal models. It was
revealed that the intrinsic link of Abnormal Savda Syndrome and depression was at the molecular level.
A-137
The construction of the Uighur pharmaceutical scientific research information support
platform system based on Cloud Technology
Zhou Yi1,2, Dilxat Yimit1, Luo Xueqiong2, Chen Guozhong 1, Xue Jie1, Du Shouhong 1, Sen Gan 1,
Nurmuhammat Amat2，Abdiryim Yusup2, Halmurat Upur 2*
1
Xinjiang Medical University; Urumqi 830011, China; 2 Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou
510080, China;
2 Traditional
Uighur Medicine Department, Xinjiang Medical University, 393 Medical University Road, Urumqi
830011, Xinjiang Uighur Autonomous Region，PR ChinaCorrespondence should be addressed to halmurat@263.net
In order to promote the inheritance and research of traditional medicine in Xinjiang fastly, this project will
construct the Uighur pharmaceutical scientific research information support platform system based on
cloud technology for Uighur pharmaceutical scientific in Xinjiang. The system's information source is
making up with the private cloud which made up with our school/our school's secondary college/hospital
site and the public cloud which made up with Domestic/foreign public website/medical professional
website. So it can collect, analysis, integration and storage the latest medical information automatically.
According to the needing of the Uighur Medicine disciplines scientific research, the cloud platform
provides the researchers with the latest information which they concerns, such as Basic Medicine,
Complexity of disease pathogenesis, Standardization of prevention in Traditional Chinese Medicine/
Uighur Medicine/Western Medicine, New medical technology, Epidemiological studies, Medical
Conference, Medical hotspots, Announcements of Project Application and so on. So that the Uighur
Medicine disciplines scientific researchers can master the latest medical research dynamic and track the
progress in their research work rapidly, comprehensively, and accurately. At the same time, the
construction of the virtual community and exchange platform can further promote the advancement of the
researchers' work and express the modern information technology to provide comprehensive, efficient
and accurate information support role in standardization of prevention of Traditional Chinese Medicine,
Uighur Medicine and Western Medicine.
A-138
Chemical Constituents in Essential Oil from Hyssopus officinalis.L by Steam Distillation
and SFE-CO2 by GC-MS
Mahsum Ekim1，
Nurjan1, Abdiryim Yusup*
1Traditional
Uighur Medicine Department, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China; *Correspondence should be addressed to abdiryim@yahoo.com
To compare chemical constituents of the essential oil of Hyssopus officinalis.L to be extracted by steam
distillation (SD) and supercritical-CO2 fluid extraction (SFE-CO2). The chemical components of the
essential oil were separated and identified by GC-MS, Their relative contents were determined by
normalization of peak area. In essential oil extracted by SD 65 compounds were detected, with the higher
levels of linoleic acid(13.83%)，
palmitic acid(13.80%),(+)- pulegone(8.31%)，
(+)the Spartak
104
alcohol(6.27%)，
20 alkyl(5.34%)，
lemongrass acid(4.07%). In essential oil extracted by SFE-CO2 34
compounds were detected, with the higher levels of the γ-Valley sterol(31.882%)，
21
alkyl(19.953%)，
linolenic acid(16.279%),hexadecane(15.939%)，
palmitic acid(13.80 %)，
linolenic acid ethyl
ester(8.766%). The constituents of essential oils extracted by SFE- CO2 were different from that of
extraction by SD. The result provided evidence for understanding the constituents of essential oils and
explitation of Hyssopus officinalis.L .
A-139
Preliminary study on the anti-inflammatory effect of the extracts of Hyssopus officinalis.L
Mahsum Ekim1，
Nurjan1, Abdiryim Yusup1*
1Traditional
Uighur Medicine Department, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China; *Correspondence should be addressed to abdiryim@yahoo.com
Objective: To study the anti-inflammatory effect of extracts from Hyssopus officinalis.L to screen the
fraction anti-inflammatory activity. Methods: Through mouse anti-inflammatory, cough, expectorant
experiment to observe the anti-inflammatory, cough, expectorant effects of Hyssopus officinalis.L. water,
ethanol extract and the ethyl acetate extract parts. Results: Compared with model group, Hyssopus
officinalis.L. water, ethanol extract and the ethyl acetate extract had anti-inflammatory, cough,
expectorant effect (P <0.05) and the role of the ethyl acetate extract was more significant. Conclusion:
The ethyl acetate extract from Hyssopus officinalis.L have favourable anti-inflammatory effect.
A-140
Abnormal Savda Munziq Improves the Biological Syndromes of CUMs induced
Depression Animal Models
Abdiryim Yusup1, Abduwayit Nurahmet1, Guzalnur Imam1, Halmurat Upur1*
1Collage
of Traditional Uighur Medicine, Xinjiang Medical University, 830011, Urumqi, Xinjiang Uighur Autonomous Region，
China. Correspondence should be addressed to halmurat@263.net
Objective: To observe the effect of Abnormal Savda Munziq (ASMq) on biological syndromes of chronic
unpredictable mild stress (CUMs) induced depression animal models. Methods: The 84 SD rats were
randomly divided into 6 groups with 14 in each group: normal control group, depression model group,
groups of Abnormal Savda Munziq granular for high, medium and low dosages (6, 3 and 1.5 g/kg.BW,
respectively) and the positive control group (Fluoxetine, 3.5mg.kg-1). Except for the normal control
group, the rats in all other groups were given chronic unpredictable mild stress (CUMs) to build the
depression animal models. The positive control group and the Abnormal Savda Munziq groups were
orally administrated for 24 days, once a day. To observe the biological syndromes such as general
states, and to measure the body weight, food intake and water intake of animals in each group. Results:
Compared with the control group, the model group animals appeared the biological syndromes such as
loss of body weight, hair disorder and dull, cooler skin, purple tongue constipated, slow weight gaining,
reduction of food intake and water intake. The biological syndromes such as slow weight gaining,
reduction of food intake and water intake of the rats in Abnormal Savda Munziq high dosage group and
positive control group were improved than that of model group. Conclusion: The biological syndromes
105
of depression animal models were improved effectively by the administration of Abnormal Savda Munziq
granular.
A-141
Abnormal Savda Munziq, an Herbal Preparation of Traditional Uighur Medicine,
Regulates the Neuroendocrine Function of CUMs induced Depression animal models
Abdiryim Yusup1, Hanzohra Upur1, Guzalnur Imam1, Halmurat Upur1*
1Collage
of Traditional Uighur Medicine, Xinjiang Medical University, 830011, Urumqi, Xinjiang Uighur Autonomous Region
China .Correspondence should be addressed to halmurat@263.net
Objective: To observe the effect of Abnormal Savda Munziq (ASMq) on neuroendocrine function of
chronic unpredictable mild stress (CUMs) induced depression animal models. Methods: The 84 SD rats
were randomly divided into 6 groups: Group 1 served as normal control, administered with normal saline.
Group 2 served as negative control, administered with normal saline. Group 3 served as positive control,
administered with Fluoxetine at 3.5mg/kg.BW. Groups 4, 5 and 6 served as ASMq high-dose, ASMq
medium-dose and ASMq low-dose group, administered with ASMq at 6, 3 and 1.5 g/kg.BW, respectively.
All the groups were orally administered for 24 days, once a day. Except for the normal control group, the
rats in all other groups were given CUMs to build the depression animal models. Rats were sacrificed at
24st day and the neuroendocrine function was analyzed for serum levels of CORT, ACTH, AD, ß-EP, NE,
BDNF, DA and 5-HT. Results: Results showed that the serum level of AD, ß-EP, NE, BDNF, DA and
5-HT in negative control group were significantly decreased (P<0.05), CORT and ACTH were
significantly increased (P<0.05). The serum level of AD, ß-EP, NE, BDNF, DA and 5-HT were increased,
and the serum level of CORT and ACTH were decreased in ASMq high-dose group (P<0.05,
respectively). Conclusion: It suggested that ASMq had antidepressant effect on CUMs induced
depression animal model by regulating neuroendocrine function of depression animal.
A-142
Clinical Research on Treatment of Knee Osteoarthritis by the instrument of Heat
Conduction Sand instrument in Uighur Medicine
He Chunhui1, Halmurat Upur1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China;* Correspondence should be addressed to halmurat@263.net
Objective: To observe the curative effect and Knee function improved integral evaluation and the
influence of the hemorheology by the instrument of heat conduction sand treatment in Uighur Medicine on
knee osteoarthritis and reducing the dosage of using western medicine, for the sake of exploring its
mechanism. Methods: The 120 cases of KOA patients were randomly divided into treatment group and
control group, 60 cases in each group: the sand treatment group: patients were treated for 30 minutes
once a day. The period of treatment was 10 days. The control group: patients received TDP radiation
therapy, its treatment method and time was the same as treatment group. Both groups took oral celebrex
capsule 200 mg once a day. Observe function evaluation scores, clinical efficacy and the influence of the
hemorheology of two groups before and after treatment. Results: compared the total curative effect of
106
two groups, the total effectiveness rate of treatment group is 91.9%; and the control group was 83.3%, the
difference was statistically significant through two groups’ comparison (P< 0.05), which indicates that both
groups can improve the knee function, but the treatment group was better than control group; Compared
the improvement of two groups’ learn index after treatment, the difference was statistically significant (P <
0.05); which explained the treatment group in improving hemorrheology index situation was better than
control group. Conclusion: 1. the instrument of heat conduction sand treatment in Uighur Medicine is
better in stopping pain, fluctuation building ability, swelling and buckling Angle and stiffness in a degree of
contracture of knee osteoarthritis treatment, its curative effect is better than the TDP radiation therapy. 2.
KOA patients often associated with abnormal blood rheology, blood is at high condensation state, Uighur
conduction heat effect sand therapy apparatus can improve the whole blood viscosity index of patients
with osteoarthritis of the knee joint, through the sand cure heating and Traditional Uighur drug absorbed
through skin, can improve local blood circulation, acetanilide detumescence.
A-143
Effect of pomegranate ethyl acetate extract on the function of rat islet cells
Yao Hai1, Yang Weixing3, Xu Yonghua4, Wang Ye1, Ren Jun5, Mao Xinmin1, Yi Jingyang1, Han Xue1, Ma
Xiaoli1, Wang Lifeng 1, Wang Changhui2, Li Linlin1*
1Xinjiang
Medical University, Shihezi Renmin Hospital, Xinjiang Uighur Autonomous Region, China; 2First Affiliated Hospital of
Xinjiang Medical University, 393 Xinyi Road, Urumqi, Xinjiang Uighur Autonomous Region, China; 3Changji Health School in
Xinjiang; 4Urumqi Military Hospital of Lanzhou District, 359 Youhao North Road, Urumqi, Xinjiang Uighur Autonomous Region,
China; 5Wyoming University, USA;* Correspondence should be addressed to yz-lll@vip.sina.com
Objective: The present study investigated the effects of pomegranate ethyl acetate extract (PEE) on the
function and cell viability of primary rat islet cells. Methods: PEE was extracted using 95% ethanol and a
series of organic solvents. Primary isolated rat islet cells were cultured in high glucose or high fat
conditions with or without PEE. Cell viability was determined by MTT assay. Insulin secretion was
assessed using radioimmunoassay. Results: Insulin levels were significantly higher in cells treated with
high glucose + PEE, palmitic acid + PEE, and olein acid + PEE compared to the cells treated with high
glucose, palmitic acid, or oleic acid alone, in which groups the insulin levels were markedly less than that
of the normal control group (P < 0.01). Conclusions: PEE promotes islet cell growth and insulin
secretion. These results suggest that PEE can protect islet cell function in both high glucose and high fatty
acid environments.
A-144
Establishment of hepatocarcinoma carrying abnormal savda animal model and it`s
morphological study
Yusup Tuerxun1, Halmurat Upur1*
1Traditional
Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China;* Correspondence should be addressed to halmurat@263.net
Objective: To establish combining hepatocarcinoma disease and abnormal savda syndromes rats
model, and investigate the effect of abnormal savda on inducing and developing process of
107
hepatocirrhosis phase in this model. Methods: Under the direction of Traditional Uygur Medicine, multi
complcated induction methods were used to establish abnormal savda syndromes rats model for three
weeks, using DEN further to establish combining hepatocarcinoma and abnormal savda disease rats
model till hepatocirrhosis occurs, the animals were anesthetized at 5, 7, 9, 11 weeks under the eyes of
venous blood, the appearance of rat liver In HE staining under light microscope and transmission electron
microscopy, liver morphology and cell ultrastructure were observed dynamically. Results: Both in the
model control group and combining disease and abnormal savda syndromes model group there are the
pathological changing process of hepatoxic lesion､hepatic proliferation､hepatocirrhosis, but the
pathological changes of the liver surface in the combining disease and abnormal savda syndromes model
group is faster than model control group. At the same time, the dropsical level of liver cell, typeless
hyperplasia, fake foliole level in liver, the ultrastructural changes of decreasing hepatin and chondriosome
in liver cells in combining disease and abnormal savda syndromes model group are than those in the
model control group. Conclusion: These findings indicate that in the process in which establishing
abnormal savda disease rat model serving as a base, using DEN furthur to establish combining
hepatocarcinoma disease and abnormal savda syndromes rats model, the abnormal savda hilit
accelerates the inducing and developing process of hepatocyte to hepatic Cellular proliferation to
hepatocellular carcinoma and this process is similar to the process of humans hepatocarcinoma. It is also
stable and reliable.
A-145
Metabonomic Studies on Abnormal Savda Patients with Type 2 Diabetes Mellitus Using
NMR Spectroscopy
Mao Xinmin1, Lang Jun2, Ma Xiaoli3, Li Linlin2, Han Xue2, Halmurat Upur1*, Eli Kurban4, Tursun Turgun4,
Mamtimin Batur3,Yunuxi Kuresi2, Wang Lifeng2, Jiao Yi2, Wang Ye2, Yi Jinyang2
1 College of Chinese Traditional Medicine, Xinjiang Medical University, Urumqi ，Xinjiang
China;
2Basic
Uighur Autonomous Region， 830011,
Medical College, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous RegionChina;
3
College of Pharmacy, Xinjiang Medical University, Urumqi 830011，Xinjiang Uighur Autonomous Region, China; 4College of
Uighur Medicine, Xinjiang Medical University, Urumqi 830011, China; *Correspondence should be addressed to
Halmurat@263.net
To investigate the metabolites changes in urine of patients carrying type 2 diabetes mellitus with abnormal
savda based on the theory of Traditional Uighur Medicine. 1H Nuclear Magnetic Resonance (NMR) based
on metabonomic analysis was performed on urine samples obtained from the abnormal savda patients
with type 2 Diabetes Mellitus and savda volunteers with normal plasma glucose. The Orthogonal Partial
Least-Squares discriminant analysis (OPLS-DA) was fulfilled to distinguish the metabolic phenotypes.
The results show that the metabolites in urine of abnormal savda patients with type 2 diabetes mellitus are
significantly changed. The concentrations of glucose, taurine, betaine are increased (P<0.05);
Meanwhile, the concentrations of some amino acids, creatinine, hippurate, citric acid and dim ethylamine
are decreased (P<0.05) as compared with savda volunteers with normal plasma glucose. The findings
indicate that there are great differences in the urine metabolites between the abnormal savda patients
type 2 diabetes mellitus and savda volunteers with normal plasma glucose by using NMR; and reveal that
endogenous compounds may be the ingredients of abnormal savda patients with type 2 diabetes mellit
108
A-146
Studies of chemical constituents and antioxidant activity of Jujubae Fructus in Xinjiang
Wang Dongdong1, Tian Shuge1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China;* Correspondence should be addressed to tianshuge@hotmail.com
Objective: To study chemical composition and antioxidant activity of six cultivars of jujubae fructus from
different areas in Xinjiang Uighur Autonomous Region, provide a more stable and reliable method for
quality control, and provide a reference for clinical medicine and food processing. Methods: Total
flavonoids and Polyphenolic contents were measured using spectrophotometry; rutin, oleanolic acid,
ursolic acid contents were measured using high performance liquid chromatography (HPLC method),
cyclic adenosine monophosphate (cAMP) contents were measured using Rapid Resolution Liquid
Chromatography (RRLC); to determine metal elements by Microwave Digestion-Flame Atomic
Absorption Spectrometry(FAAS); to optimize the extract technology of total flavonoids from jujubae
fructus; to study Separation and purification process of total flavonoids from jujubae fructus using
macroporous adsorption resin; to utilise three types of antioxidant activity models (clear DPPH free
radical, hydroxide radical and SOD active effect) to do antioxidant activity screening to water extracts of
jujubae fructus. Results: There were different levels of total flavonoids and polyphenolic contents in six
cultivars of jujubae fructus; the contents of rutin, oleanolic acid, ursolic acid and cyclic adenosine
monophosphate in six cultivars of jujubae fructus in Xinjiang existed obvious difference; the metal
element contents in six cultivars of jujubae fructus were determined; through 2 times of refluxing with 70%
alcohol, of which the amount was 12 times of sample, and 1h for each refluxing , the best extraction
efficiency was obtained. The experimental results of the static and dynamic adsorption-desorption
experiments show that D101 resin possesses higher absorption and desorption capacity. The results of
the present work indicate that the water extracts from six cultivars of jujubae fructus had different levels of
antioxidant activity. Conclusion: The study determined contents of total polyphenolic, total flavonoids,
rutin, oleanolic acid, ursolic acid, cyclic adenosine monophosphate and metal elements in six cultivars of
jujubae fructus, optimized the extract technology of total flavonoids and studied separation and
purification process of total flavonoids from jujubae fructus using macroporous adsorption resin, studied
antioxidative actions of water extracts from six cultivars of jujubae fructus in vitro, and offered theory
evidences for quality control and further study of jujubae fructus.
A-147
Studies on Chemical Constituents and anti-tumor of essential oil from the seeds of Ferula
sinkiangensis
Wang Yue1, Jiang Lin1, Li Xiaojin1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to xjlxj@126.com
Objective: For the further study of the seeds of F.sinkiangensis pharmacological effect and to lay the
foundation for its exploitation, the chemical composition and naphtha antitumor activity of xinjiang ferulic
seeds were researched in this paper. Methods:1)The extracted mixture of the seeds of F.sinkiangensis
109
from purified water,95％
ethanol and petroleum ether were studied by test tube and filter paper method. The
chemical constituents of the seeds of F.sinkiangensisK is primarily demonstrated by precipitation reaction
or color reaction with various indicator.2) The constituents were isolated and purified by column
chromatographies and their structures were identified by physicochemical properties as well as spectral
data.3); The essential oils were extracted by the method of hydrodistillation and analyzed by gas
chromatography-mass spectrumetry (GC-MS). 4) The conventional MTT method was used to analyse
essential oils inhibitory action face CACO2 proliferation in vitro and count IC50. Results: 1) The
preliminary test indicates that the seeds of F.sinkiangensisK may contain chemical constituents such as
volatile oil, triterpenes, sterides, coumarin, terpene lactone, flavonoids, anthraquinones, phenols, amino
acids,sugar etc. 2) Twelve compounds were isolated from the seeds of F. sinkiangensis and their
structures were elucidated as stigmasterol(Ⅰ
), siknianone(Ⅱ
), stigmasterol-3-O-glucoside(Ⅲ
), colladonin(Ⅳ
),
ferulic acid(Ⅴ
),7-hydroxy oumarin(Ⅵ
). 3) 26 compounds were identified in F.sinkiangensis, comprised
99.001% of total essential oil; 21 compounds were identified in F.fukangensis, comprised 100% of total
essential oil; 25 compounds were identified in Ferula ovina, comprised 99.459% of total essential oil.
n-propyl sec-butyl disulfide is the main component in three cultivars seeds of Resina Ferulae,accounting
for 55.875%、
49.797%、
53.781%, respectively. 4) the essential oil from the seed of three Xinjiang Ferula
asafetida to the CACO2 have a dose dependent effect in vitro, the essential oil IC50 of F.
snkiangensis、
F. fukangensis、
F. ovina were16.82±1.17 ug/ml、
12.28±1.41μg/ml、
10.75±1.02μg/ml,
respectively. Conclusion: 1); This experiment provide foundation for further study of its bioactivities to
preliminarily determine the chemical components of the seeds of F. sinkiangensis. 2) Compounds
Ⅰ
,Ⅲ
,Ⅳ
,Ⅴ
,Ⅵ
were isolated from the seeds of F. sinkiangensis for the first time. 3) Compared the essential oil
chemical constituents with the seed Three Xinjiang Ferula asafoetida, we can see the bases of three
asafetida are the same , with little diversity among three cultivars seeds of Resina Ferulae from Xinjiang.
4) The essential oil of this three kinds of Xinjiang F. asafetida seeds have strong anti-tumor activity in vitro,
for the size of the tumor suppressor role in turn F. ovina> F. fukangensisK> F. snkiangensis K.
A-148
Study on Quality Standard and Fingerprint of Ziziphora clinopodioides Lam
Yu Qian1, Tian Shuge1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to tianshuge@hotmail.com
Objective: This study was designed to examine the chemical composition and antioxidant activity of
Uighur Medicine Ziziphora clinopodioides Lam., to further expand the pharmacology effect of Ziziphora
clinopodioides Lam. and to establish firm fundament of reasonable developing and utilizing to Ziziphora
clinopodioides Lam. in Xingjiang. Methods: According to the 2010 version of Pharmacopoeia, there are
identifications of the original plant of ziziphora clinopodioides lam and descriptions of their properties; It's
stems, leaves and powder for microscopic identification, it's water extract and alcohol extract of the
physical and chemical identification and in contrast to linarin, caffeic acid and rosmarinic acid on TLC. The
7 batches of ziziphora clinopodioides lam of moisture and ash were determined. GC / MS techniques are
used to analyze four different origins Ziziphora clinopodioides Lam essential oil chemical composition,
and the method of liquid chromatogram to determine contents of pulegone, caffeic acid, rosmarinic acid,
diosmin, Linarin in Ziziphora clinopodioides Lam. from ten different origins of production have been
110
applied in Xinjiang to study on fingerprint of Ziziphora clinopodioides Lam. Results: Check in formulating
moisture was less than 9%. Total ashes were less than 12%. Acid-insoluble ashes were less than 4%.
9-20 compounds identified from the essential oil, the contents of pulegone, caffeic acid, rosmarinic acid,
diosmin, Linarin in Ziziphora clinopodioides Lam. from ten different origins of production in Xinjiang are
obviously different. The determination of the acquisition of ten different origins of Ziziphora clinopodioides
Lam, calibration of the 11 common peaks, and the establishment of the Ziziphora clinopodioides Lam
fingerprint chromatogram were made. Conclusion: This project conducted a preliminary study for
Xinjiang origin Ziziphora clinopodioides Lam quality standards. The study determined contents of
pulegone, caffeic acid, rosmarinic acid, diosmin, Linarin in Ziziphora clinopodioides Lam from ten different
places of production in Xinjiang, and laid the foundation for the lip herbs to establish the standards of
scientific quality. Meanwhile, the fingerprinting studies were firstly conducted to Ziziphora clinopodioides
Lam. It offered scientific evidences for establishing scientific quality standard of Ziziphora clinopodioides
Lam.
A-149
Study on the Uighur Medicine Compound of the Anticancer Activity Parts of Abnormal
Black Munziq—Based on the “TCM Components” Mode
Yang Juan1, Zhang Fan1, Halmurat Upur1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China;* Correspondence should be addressed to halmurat@263.net
Objective: The purpose of this paper is to make the preparation of the anticancer activity parts of
Abnormal Black Munziq study on its chemical components and determine the main components’ content
to evaluate its material composition, prepare its standard components and proceed chemical
characterization, analyse the quantitative and qualitative, and create Information database. Method: 1.
Gradient elution on Abnormal Black Munziq to obtain its anticancer active parts using polyamide column
chromatography and solid phase extraction technology. We used polyamide column chromatography to
prepare the extract of Abnormal Black Munziq, used solid phase extraction, and separated the extract to
prepare the anticancer activity parts. 2. We prepared the standard components of anticancer activity parts
by using semi preparative high performance liquid chromatography. 3. We proceed chemical
characterization with standard components and used UV and HPLC. 4. We analyzed the qualitative of
anticancer activity parts of Abnormal Black Munziq and used TLC. 5. We analyzed the quantitative of the
anticancer activity parts and used HPLC. 6. We drew up Prescription database software, input all kinds of
information of Abnormal Black Munziq and established Data-base using C# database programming
language. Results: 1. the anticancer activity parts of Abnormal Black Munziq were acquired. 2. 18
standard components of the anticancer activity parts of Abnormal Black Munziq were prepared. 3. The
standard components to proceed chemical characterization of UV and HPLC were directed. 4. The
qualitative analysis result of TLC indicated that the anticancer activity parts of Abnormal Black Munziq
contained gallic acid、
caffeic acid、
rutin、
licorice glycoside、
quercetin- 3- O- glucoside、
isoferulic
acid、
quercitrin、
hesperidin、
rosmarinic acid、
glycyrrhizic acid. 5. The result of quantitative analysis indicated
that every gram of the Anticancer activity parts of Abnormal Black Munziq contained gallic acid
4.85mg、
caffeic acid 6.99mg、
rutin 27.23mg、
licorice glycoside 20.24mg、
quercetin- 3- O- glucoside
12.25mg、
isoferulic acid 16.08mg、
quercitrin 7.75mg、
hesperidin 58.16mg、
rosmarinic acid
57.96mg、
glycyrrhizic acid 19.29mg. 6. The information database of Abnormal Black Munziq was
established. Conclusion: 1. Abnormal Black Munziq with gradient elution using polyamide column
chromatography and SPE is a simple and effective way to acquire its anticancer activity parts. 2. To
111
complex things, the most simple and direct way to understand them is decomposition and simplification.
Semi preparative high performance liquid chromatography, which has the feature of high-efficiency,
speediness and automatic operation has an important position in the area of separation and purification.
3. Systematic chemical characterization of Traditional Chinese Medicine composition is a way of using
the modern separation technology and method to proceed scientific and effective characterization
research. UV and HPLC, which play an important role in the area of complex samples analysis are also
the most powerful tools to solve the problem of the area. 4. The anticancer activity parts have high content
of flavonoids substances. Qualitative analysis by TLC have advantages of shortcut, trace, high sensitivity
and good separation efficiency and so on. 5. On the anticancer activity parts of Abnormal Black Munziq
we undertook quantitative analysis of gallic acid、
caffeic acid、
rutin、
licorice glycoside、
quercetin- 3- Oglucoside、
isoferulic acid、
quercitrin、
hesperidin、
rosmarinic acid、
glycyrrhizic acid and used HPLC. The
methods are accurate, precise and are all consistent with the requirement of content determination, it is a
special and practical quantitative analysis method. 6. By using the information database to analysis and
characterize the Chinese herbal medicine and compounds of traditional Chinese Medicine, we supplied
evidences for quality control of medicine and standard components, provided information supporting
platform for the high-efficiency and in-depth research of traditional Chinese Medicine’s pharmacodynamic
material basis and the development of components of traditional Chinese Medicine.
A-150
The impact of Hypoglycemic Ziyabiti Tablet (HZT) extracts on type 2 diabetes mellitus
rats and its activity in cultured liver cells
Li Guirong2, Fan Yong2, Wang Xiaofeng3, Mao Xinmin1, Yi Jingyang1, Han Xue1, Ma Xiaoli1, Wang
Lifeng1, Ren Jun1, Wang Ye1*, Li Linlin1*
1Department
of Pharmacology, Xinjiang Medical University, Urumuqi, 830011Xinjiang Uighur Autonomous Region China; 2First
affiliated hospital of Xinjiang Medical University, Urumuqi 830011 ， Xinjiang Uighur Autonomous Region, China; 3China
Pharmaceutical University, Nanjing, China;* Correspondence should be addressed to wy2965@.sina.com (Ye Wang);
yz-lll@.vip.sina.com (Linlin Li)
Objective: The present study aimed to investigate the impact of Hypoglycemic Ziyabiti Tablet (HZT)
extracts on type 2 diabetic rats as well as its activity in cultured liver cells. Methods: Wistar rats were
given a high fat emulsion for 10 days, and their insulin resistance was assessed using a blood capillary
method. Alloxan (ALX) was intraperitoneally injected for two subsequent days to induce the type 2
diabetes mellitus (T2DM) in rats. The T2DM rats were divided into 5 groups: saline (20ml·kg-1·d-1),
positive control (Xiaoke Pills 420mg·kg-1·d-1) and groups treated with different dosages of HZT (220,440,
and 880 mg·kg-1·d-1). Fasting blood glucose (FBG), total cholesterol (TC), total triglycerides (TG),
high-density lipoprotein (HDL-C), fasting insulin in serum (FINS), insulin secretion (IS) and insulin
sensitivity index (ISI) were measured. Results: HZT at 220 and 440 mg·kg-1·d-1 decreased the FBG and
TC levels (P﹤
0.05); HTZ at 440 mg·kg-1·d-1 and XKP (Xiaoke pills) at 420 mg·kg-1·d-1 increased the
FINS level (P﹤
0.01), and HZT at 220 and 440 mg·kg-1·d-1 and XKP increased IS level (P﹤
0.05). The vitro
studies showed that HZT at 0.1, 0.2, and 0.4mg/ml decreased the LDH activity and promoted the glucose
consumption in a L-02 Par model. Conclusion: The hypoglycemic mechanism involving HZT is possibly
contributed to the promotion of insulin secretion in the pancreas and increased utilization of glucose in the
liver.
A-151
112
The study on the development of the Xipayisolid-gingival buccal tablets
Ma Pingping1, Nie Jihong1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China;* Correspondence should be addressed to xjnjh411@163.com
Objective: To definite the technology of preparation, establish the quality standards and proceed the
preliminary pharmacodynamics studies of xipayi solid- gingival buccal tablets. Methods: 1) To determine
the Gallic extraction process parameters based on the composite index tannin, gallic acid and extraction
yield ; 2) To determine the Gallic purification process parameters based on the composite index tannin,
gallic acid and extraction yield ;3) To research the moulde technology of xipayi solid- gingival buccal
tablets;4) Employ thin layer chromatography to qualitation discrimination, using the method of HPLC to
determine the contents and institute the quality criteria to the produce obtained from the followed
preparation technology; 5) To evaluate its effectiveness according to the preliminary pharmacodynamics
studies. Result: 1) Optimizing extraction conditions by adding 8 times the amount of water ,extracting
three times, each 2.5 hours; 2) Optimizing refining process by concentrating the extraction to medicine:
liquid (1:5), placed in low temperature (4 ℃
) frozen 24 hours, using the clear supernatant as sample; 3) The
moulde technology of xipayi solid- gingival buccal tablets was used 7 times Sugar power,10%
microcrystalline cellulose, 3‰ beetsugar as supplementary material and 93% ethanol as bond, adding
6% menthol of ethanol liquid and 1% magnesium stearate to press tablets; 4) Establishing the preparation
of gallic by TLC method and quality standards by HPLC, the method of gallic acid with HPLC: mobile
phase: methanol - tetrahydrofuran - acetic acid - water (0.08:0.64:2:97.28) in mobile phase and the
detection wavelength of 271nm, column temperature:35 ℃
, the flow rate:1ml/min. ; 5) Preliminary
pharmacodynamic showed that, significant inhibition roles in xipayi solid- gingival buccal tablet induced
ear swelling, pain caused by heat stimulation in mice, rats phenol oral ulcers. Conclusion: The
preparation technology of xipayi solid- gingival buccal tablets is stable, reasonable and feasible process.
The quality standard is convenient, accurate, and can be used to quality control. Preliminary
pharmacodynamic results showed better roles in anti-inflammatory agents, analgesic and promoting
healing of mouth ulcers.
A-152
Toxicological evaluation aqueous extract of the galles of Quercus infectoria olivin rodent
Mubarak1, Nurmuhammat Amat2*, Kasimjan, Halmurat Upur2*
1
Pharmaceutical Department, Xinjiang Medical University, 830011-Urumqi, Xinjiang Uighur Autonomous Region, People’s
Republic of China; 2Traditional Uighur Medicine Department, Xinjiang Medical University, 830011-Urumqi, Xinjiang Uighur
Autonomous
Region,
People’s
Republic
of
China ； *Correspondence
should
be
addressed
to
Nurmuhammat.Amat；
E-mail：
halmurat@263.net，
nur818@hotmail.com
Objective:Galls of Quercus infectoria Olivier (Fagaceae) is one of the most popular medicinal plants in
Uighur Medicine. The aqueous extract preparation of Q. infectoria galls is widely used traditionally in
Uighur Medicine for treatment ulcerative colitis and hemorrhoid etc but to date there is no documented
evidence corroborating its safety. The present investigation was carried out to evaluate the safety of an
aqueous extract of the Q. infectoria galls in rodent by determining its potential toxicity after acute and
113
chronic administration in rodents. Methods: For the acute study, an aqueous extract of the Q. infectoria
galls was administered to ICR mice in doses (5-10g/kg body weight) given by enema. General behavior
adverse effects and mortality were determined for up to 14 days. In the sub-chronic dose study, the
aqueous extract preparation of Q. infectoria galls was administered rectally at doses of (5-10g/kg body
weight) daily for 180 days to Wistar rats. Otherwise it has one month convalescent period after the
discontinuation. Selected biochemical、
hematological and histopathological parameters were determined
after180 days. Results: In the acute study in mice, acute intake of extract up to 10 mg/kg did not cause
any mice was death so disable to calculate LD50, also did not produce mortality or significant changes in
general behaviour. Rats given oral doses of Q. infectoria galls-extract (10、
7.5 、
5.0g/kg) survived the 6
months of dosing (i.e. LD50 much higher than 1 g/kg), experienced no significant changes in general
behaviour and hematological and biochemical parameters, except for transient decrease in AST activity.
No significant changes were observed in organ weights, and histopathological results showed normal
profile suggesting no morphological alterations. Because of the relatively high NOAEL values in the acute
study in mice, and lack of mortality or clinically significant changes in the biological and hematological
parameters in rats after 180 days of daily dosing, it may be concluded that the aqueous extract
preparation of Q. infectoria galls does not appear to have significant toxicity. Conclusion:In view of the
doses consumed empirically in traditional medicine in Morocco, there is a wide margin of safety for the
therapeutic use of Q. infectoria galls.
A-153
Dialectical Analysis of exercise-induced fatigue patients according the Uighur Medicine
humoral quality
Ailyjan Asla, Meng Qingcai1, Liu Zhenfeng1, Fang Rui1*
1
Affilated Tradional Chinese Medicine Hospital ,Xinjiang Medical University, Xinjiang Uighur Autonomous Region, 83000,
Urumqi, China; *Corresponding to xjfr@163.com
The exercise-induced fatigue is a phenomenon caused by the body movement itself, "the body's
physiological processes can not be continued to hold its function in a particular level and / or unable to
maintain a predetermined exercise intensity" of the body decreased exercise capacity. Uighur Medical
body fluids qualitative theory that the body fluids qualitative abnormalities is the main reason to cause
changes in human physiology, psychology, pathology, function. That exercise-induced fatigue Uighur
Medical abnormal humoral quality areas. This article focuses on exercise-induced fatigue and Uighur
Medicine humoral quality links discussed from the perspective of the Uighur Medical fluid transfer theory,
and exercise-induced fatigue body fluid quality type for Analysis.
A-154
Comparative study Uighur Medicine sand therapy with other regional sand therapy，
sand
characteristics
Abudusalam Saidula1, Osman Yasin1, Satar Kurban1, D.Mahemuti 2
114
1Uighur
Medicine Hospital of the Turpan area, Turpan, 838000, Xinjiang Uighur Autonomous RegionChina; 2Department of
Mechanical Engineering, Xinjiang University, Urumqi , Xinjiang Uighur Autonomous Region，830049, China
The mechanism of the Uighur medical sand therapy and sand characteristics are inherently tied. We have
done the comparative study of the characteristics of Uighur medical sand therapy with other regional sand
therapy sand in this paper. The results show that the magnetic mineral content of the sand of Turpan has
more than another area sand, also the specific weight is larger, the particles of sand is relatively uniform,
Magnetic ore particles is larger ,and with strong magnetic, showing the gray and black. The results of this
study provide a scientific basis to further explore the mechanism of the Uighur medical sand therapy.
A-155
The Relationship between toxic electron microscopy examination and alkaloid amounts
in Datura stramonium L seeds of Xinjiang
R.Islam 1, Y.Mamat 1, A.Abdugheni 1,Yanming1, S.Abay 1
1Inistitute of Xinjiang Traditional Uighur Medicine, 776, YaAn Road, Urumqi, 830049, China; 2Laboratory of
Traditional Uighur Medicine Prescription of Xinjiang, 776 ,Yan An Road , Urumqi 830049
Objective: To investigate the relationship between toxic Electron microscopy examination and alkaloid
Amounts(atropine,scopolamine) in Datura stramonium L seeds(crude and extraction drug ) of Xinjiang.
Methods: SD rats were randomly divided into solvent control group, extraction of Datura stramonium L
seeds group (1.55,3.10,6.20g crude drug·kg-1),Datura stramonium L seeds crude drug group(3.10 g
crude drug·kg-1) ,each group orally was given 1ml·100g-1 for 90continuous days. then to observed
Electron microscopy examination of liver ,kidney ,brain and also the alkaloid amounts were
tested.Results: Results showed that Datura stramonium L seeds(crude drug) all animals and extraction
of Datura stramonium L seeds(6.20g crude drug·kg-1) half animal can cause drug-induced liver injury,
and the alkaloid amounts in Datura stramonium L seeds(crude and extraction drug) were 0.2859
mg/g,0.8136mg/g;2.1178 mg/g,4.1700 mg/g. Conclusion: Datura stramonium L seeds(crude and
extraction drug) can cause liver damage, but the damage degree does not direct ratio with the alkaloid
amounts.
A-156
Effect of psychological factors on vitiligo patients’ hypothalamus-pituitary-adrenal axis
and intervention study
Aikebaier Anzhaer 1, Silafu Aibai 1*, Tuerxun Wufuer 1, Ayinuer Aimaiti 1, Aizezi Yasen 1, Guzainuer
Kuerban 2, Julaiti Abuduwayiti 2, Awaguli Haibibula 3
1 Uighur Medicine Hospital of Xinjiang Uighur Autonomous Region, 830049,
2Uighur
Urumqi, Xinjiang Uighur Autonomous Region，China;
medicine skin disease key subjects of State Administration of Traditional Chinese Medicine, 830049, Urumqi, Xinjiang
Uighur Autonomous Region，China; 3Uighur medicine vitiligo diagnosis and treatment key unit of State Administration of
Traditional Chinese Medicine, 830049, Urumqi, Xinjiang Uighur Autonomous Region，China; *Corresponding author: Sirap.Abai,
Email:aibai@263.net
Objective: Associate and analyize anxiety and depression psychological factors involved in the
115
occurrence and development of vitiligo, and to explore its affect on patients’
hypothalamus-pituitary-adrenal axis (HPAA), and the role of psychological interventions to reduce
symptoms and improve efficacy. Methods: Different stages of vitiligo patients using the Hamilton Anxiety
Scale (HAMA) and Depression Scale (HRDS) score, and using radioimmunoassay for the determination
of serum CRH, ACTH, Cortisol level were used to compare with the normal control group and contrast
patients who had psychological intervention to those who did not had. Results: (1) Anxiety and
depression of vitiligo patients who are in advanceing and stable phase are significantly severe than
normal contrast, stable phase are less severe than advanceing phase, but have no significance. (2) In the
assay for the HPA axis hormone levels, stable phase patients’ cortisol levels are significantly lower than
advancing phase patients’ and normal person’s, the differences are statistically significant. (3) After
psychological intervention, the intervention group patients’ efficacy and HAMA, HRSD score was
significantly lower than non-intervention group. Conclusion: Incidence of vitiligo is closely related to
changes in patients’ Neuroendocrine-Immune network, HPA axis changes in hormone levels play an
important role on the pathogenesis, including stress factors like anxiety and depression which make a
variety of hormones body changes in the body, The mechanism may be as follows: Cortisol levels of
vitiligo patients with high expression can induce the activity of liver tryptophan pyrrole enzymes, the latter
degradation of tryptophan in the blood, resulting in the lack of central tryptophan and 5-HT synthesis
decreased. Trigger depression, obsession, anxiety and other symptoms in turn to affect the tyrosine
metabolism, cause or aggravate vitiligo. But that is before vitiligo or follow-up reaction of vitiligo, which
remains to be studied further. Meanwhile, patients who had psychological intervention on the basis of
Traditional Uygur Medicine comprehensive treatment reduced the pressure on the patients, so that the
patient's quality of life improved significantly and also improved efficacy of Uygur Medicine’s
comprehensive treatment.
A-157
Arthroscopic Debridement Combined with Uygur Maizhuni Treatment of Knee
Osteoarthritis andomized Controlled Trials
Yuan Guoxiang1, Lu Yong1*
1
Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region，
China; *Correspondence should be addressed to xjluyong@163.com
Objective: To observe the clinical efficacy of Uygur maizhuni combined with arthroscopic debridement
treatment as early as the mid knee osteoarthritis and surgery before and after the medication in patients
with synovial fluid and serum expression of MMP-1, 3,13 and TIMP-1 and IL-1the level of determination of
Uygur maizhuni treatment as early as in advanced knee osteoarthritis may be the mechanism.
Methods: 90 cases of patients with knee osteoarthritis were randomly divided into experimental group
and control group, 45 cases in each group .The experimental group in arthroscopic knee clean up with 3
days after Uygur maizhuni treatment control group to be after three days of placebo, 4, 8 and 12 weeks
after surgery, symptoms, signs integral, the VAS, as the value of law and security indicators to assess and
detected before surgery and 12 weeks after surgery in patients with synovial fluid and serum of MMP-1,
3,13 and TIMP-1 and IL-1 level. Results: Postoperative efficacy of 12 weeks and found that the
experimental group total effective rate was 97.8%, the total effective rate was 80.0%, 93.3% of the total
effective rate of the control group, the total effective rate was 57.8%, statistically significant differences
116
between the two groups(P <0.05) after the end of the treatment, the serum MMP-3, TIMP-1 in serum and
synovial fluid with the control group after the treatment group compared to the difference was statistically
significant (P <0.05).Conclusion: Compared with simple arthroscopic debridement, the arthroscope with
the dimension drugs buy Uygur maizhuni treatment of early osteoarthritis of the knee of the medium-term
efficacy is more significant, especially for patients with swollen joints, pain, joint activity, playing soft legs
and other symptoms and signs improved significantly, but its mechanism of action and long-term efficacy
remains to be further experimental studies and clinical observations to verify and prove.
A-158
Association analysis Uygur Medicine's combined modality therapy with ordinary
psoriasis patien's cytokine and immunologic mechanism
Riziwan Wumaier 1, Aikebaier Anzaer 1, Silafu Aibai 1*, Tuerxun Wufuer 1, Ainuer.Aimaiti 2, Aizezi Yasen 2
Uygur Medicine hostipal of Xinjiang Uygur Autonomous Region, 830049, Urumqi ，Xinjiang Uighur Autonomous Region，China;
The Key Subject of Uighur medicine’s Dermatology of State Administration of Traditional Chinese Medicine, 830049, Urumqi
Xinjiang Uighur Autonomous Region，China; *Corresponding author: Sirap.Abai, Email:aibai@263.net
Objective: On the basis of previous study, unite Uygur-medicine's theory with latest disease pathologic
mechanism study，
observe representative cytokine of Th1 and Th2 cell in ordinary psoriasis patient’s
serum and possible mechanism. Methods: Five cytokine including IFN-γ、
IL-2、
TNF-α及
IL-10、
IL-4 of 80
ordinary psoriasis patient’s(before and after combined modality therapy of Uygur-medicine ) and 60
healthy volunteer’s peripheral blood serum are assayed by enzyme linked immunosorbent assay(ELISA)
for Association analysis. Results: (1) before treatment, contrasted to the healthy volunteer’s, the IFN-γ、
IL-2、and TNF-α concentration levels of ordinary psoriasis patient are higher, in which, IFN-γand IL-2
have extreme significance(P<0.001). TNF-α、
IL-8、
IL-2、
IL-6 levels are lower, which have extreme
significance（
P IFN-γ=0.043，
PIL-2=0.008）
. IL-10、
IL-4 significantly lower than the healthy contrast's;
(2)after a course of combined modality therapy,IFN-γ、
IL-2、
TNF-α and IL-10、
IL-4 level of ordinary
psoriasis patient have no significance contrast to the healthy volunteer’s, but IL-2 level still has.
Conclusion: Vitiligo patients have Th1 type cytokines advantaged, CD4+T cell
subgrup(Th1/Th2)unbalanced differentiation and their secreted cytokine are correlation factor of ordinary
psoriasis's initiation and development, Uygur-medicine's combined modality therapy can significantly
improve the unbalance status mentioned above via multiple targets.
A-159
Study On Xanthine Oxidase Inhibitory Actvity In Capparis Spinosa.L By
Spectrophotometry
A.Rahman 1, A.Tax 2, N.Amat 1*
1Traditional
Uighur Medicine Department, Xinjiang Medical University, 830011-Urumqi, Xinjiang Uighur Autonomous Region，
People’s Republic of China；*Correspondence should be addressed to Nurmuhammat.Amat，nur818@hotmail.com
Objective：Xanthine oxidase (XOD) inhibitor, is an important class of anti-gout drug, seek strong XOD
inhibitory activity of natural drugs and their active ingredients have great significance. In this study, a
comparative study of Capparis spinosa .Ldifferent parts of different solvent extracts in vitro xanthine
oxidase inhibitory activity of the active site was done to look for Capparis spinosa. Lrole of inhibition of
xanthine oxidase (XOD) and to provide the further development dimension of the treatment of gout drug
117
new drugs basis. Methods: UV spectrophotometric determination of Capparis spinosa .L different parts
of different solvent extracts (cyclohexane extract, dichloromethane extract, methanol extract) inhibition
of xanthine oxidase activity. Results: The Capparis spinosa L different solvent extracts of fruit ,leave
and flower extracts displayed xanthine oxidase inhibitory activity. In 100mg/mL concentration, the
medicine in three parts (leaves, fruits, flowers) in three different solvent extracts (cyclohexane extract,
dichloromethane extract, methanol extract) to varying degrees, for XOD inhibition effect, and leaves
cyclohexane extract, fruit cyclohexane extract, leaf dichloromethane extract, fruit methanol extract,
flower methanol extract inhibits the rate of more than 50%; concentration of 50 mg/mL, six different
solvent extracts showed inhibition and which three extracts exhibited higher than 50% inhibition rate;
while three extracts presented activity at 25mg/mL,with none having greater than 50%inhibition. The
most two active extracts were the fruit cyclohexane extract ,leaves cyclohexane extract,with IC50
value of122.50mg/mL,127.66 mg/mL. Conclusion: The fruits of cyclohexane extract, leaves
cyclohexane extract is activity part of Capparis spinosa L medicinal plants inhibition of xanthine oxidase,
can be further purified the active compound.
A-160
Clinical effecacy observation Uighur Medicine Preparetion Denar on the patients with
viral hepatitis with Cholestatic syndrome
Dmytriy Sebrebryakov
Turkministan State Medical University, Ashkabad, Turkministan
The treatment of cholestatic forms of viral hepatitis is the one of the complicated question in hepatology, the
purpose of this reasearch is application of the Uighur Medicine Preparetion Denar decoction treatment the
hepatitis with Cholestatic syndrome, Denar decoction can reliefe the fever of the liver, remove toxin, remove
sputum, functionging as a choleretic , laxative and uriginative agent. There was 39 patients with chronic viral
hapatitis with cholestatic sysndrome was under observation. The 15 of them were with chronic viral hapatitis
HBV+HDV, 18 with the chronic viral hapatitis HBV+HCV, and 6 with the chronic viral hapatitis HBV.The
diagnosis was verified by determination of infection marker of viral hepatitis.Along with the basis treatment
the “Denar ” was prescribed three times a day by 3-5g deluated with warm water.The course of therapy
consisted of 21 days.The control group conisited of 20 patients with the cholestatic form of chronic viral
hepatitis during the basic therapy.The effeciency of therary was evaluated by laboratory data ,there was
noted amelioration during the usage of “Denar” .Many of the patients faster then in control. Lowering of the
skin itch intensity, normalize sleeping. During the treatment , Ictertitiousness of the coverlet sypntom of 30 of
patients had decreased or then disappeared ,and fast reverse dynamics of biochemical tests, for the 28
patients the biochemical markers was improved ,also correlated with immune parameter was normalized. 9
patients at the end of treatment had the marker of the viral hepatitis decreased or show negative (superficial
antigin of viral hepaptitis HBV, antibodies IgM to the heart shaped antigen of viral hepaptitis HBV in the
immune-enzyme analysis) and there was no datected nucleic acids of viruses in polymerase chain reaction.
We also found that can not find any side effection and allergic affection. Thus, the inclusion to the complex
therapy preparation “Denar” allowed to get convincing clinical effect. The obtained results in application of
this medicine in treatment of viral hepaptitis with cholestatic syndrome syndrome present itself very
prespective.
118
A-161
Clinical Observation of Wujing Ointment in Reducing Adverse Reactions attached to
Asthenia of Spleen and Pathogenic damp of FOLFOX4 in Treating Cancer of Colorectum
Xiao Chunxia 1, Zhang Hongliang 1,2*
1Affilated
Traditional Chinese Medicine Hospital,2Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi
830011, Xinjiang Uighur Autonomous Region，China; *Correspondence should be addressed to zhanghongliang@csco.org.cn
Objective：
To observe the clinical effects of Wujing Ointment in educing adverse reactions and initially
explore its mechanism of action by treating the patients of Cancer of Colorectum attached to Asthenia of
Spleen and Pathogenic damp with FOLFOX4. Methods：
76 cases who meet the diagnosis standard of
Cancer of Colorectum from our co-operation hospitals were domized into two groups (38 cases
respectively)：
the control group which FOLFOX4 only was given and the treatment group used Wujing
Ointment at the same time.，
60g/d，
three times a day. After one cycle, we surveyed the quantity and the
descenting rate of WBC、
the quantity and the descenting rate of Hg、
incidence rate of nausea and
vomit、
the changes of syndromes of TCM、
Karnofsky scores、
immunity and completed rate of
chemotherapy.Results：
the WBC quantity of two group decreased after chemotherapy ，
the descenting
rate of WBC of the treatment group is lower than the control group，
and the difference was significant
statistically（
P＜
0.05）
；
The descenting rate of Hg of two groups and the degree has no statistical difference
(P＞
0.05). The syndromes of TCM of two groups all increased after chemotherapy (P＜
0.05).and there was
different statistically (P＜
0.05)；
The Karnofsky scores of two groups all increased after chemotherapy
(P＜
0.05).and there was a statistical difference (P＜
0.05)；
The immunity function after chemotherapy were
higher than the before,and the differences were significant statistically (P<0.05). The completed rate of
chemotherapy
of
two
groups
was
higher
than
85%，
and
there
was
no
different(P＞
0.05).Conclusion：
1.Wujing Ointment can boost patients′ immunity after FOLFOX4 and can
elevate CD4、
CD4/CD8、
NK，
and reduce CD8；
Wujing Ointment can lower the descenting rate of
WBC；
Wujing Ointment can improve the patients′ KPS.2. Wujing Ointment can improve the TCM clinical
symptoms of asthenia of spleen and pathogenic damp due to chemotherapy and improve the patienrts′
quality of life.
A-162
Clinical research of auxiliary therapy effect of Turnips on tuberculosis patients
A.Pattar 1, Nurmuhammat Amat1，
Halmurat Upur1*
1Public
Health Institute, Nutrition Department, Xinjiang Medical University, Urumqi, 830011，Xinjiang Uighur Autonomous
Region; 2Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China; *Correspondence should be addressed to Halmurat@263.net
Objective: Brassica campestris L. is cruciferous brassica roots of plants. One of traditional Uighur
Medicine for lung disease drug used along with one of the plant resources of food, widely planted in the
northwest of the Tarim Basin, Xinjiang, The Qmagur (Referred to as QMG) is Uighur Medicine name of
Brassica campestris L. The QMG both into the dishes, they can do the diet, people in the past two
thousand years accumulated a wealth of treatment, health care knowledge and methods. A Tuberculosis
(TB) is caused by Mycobacterium tuberculosis and malnutrition-related infectious diseases, tuberculosis,
119
once widespread in the world, especially the economically backward, living in poor areas. Among TB
patients and 88.6% with malnutrition, malnutrition is the lack of calories and protein caused by a nutritional
deficiency. The number of TB patients in China ranks second in the world, which is one of the world's 22
TB high-burden countries. Smear-positive prevalence and mortality in patients with active pulmonary
tuberculosis morbidity in Xinjiang are 1.3 times the national average, 1.5 times and 3.9 times, especially
the southern border region is more prominent. In short, the TB epidemic in Xinjiang is still very severe, an
increasingly large number of patients, the huge cost of treatment and prevalence of low quality of life has
become a social burden, therefore tuberculosis has become the most important public health problem in
Xinjiang. In order to clarify the QMG on the role of adjuvant therapy for patients with pulmonary
tuberculosis, we screened for tuberculosis patients for nutritional intervention studies. Methods:
Pulmonary TB nutrition intervention: nutrition intervention study screening patients 137 patients were
divided into control group and intervention group. The intervention group was given the QMG honey
cream 3, observed changes in the indicators between the two groups before and after the intervention.
QMG honey paste on immune function: CP induced immunocompromised animal models of nutritional
intervention. Results: The results showed that pulmonary TB patients after the intervention of weight and
BMI were significantly higher (P<0.01), cough, sputum, fever, night sweats, loss of appetite and weight
loss and other symptoms improve (P<0.01 and P <0.05), X-line and sputum smears, etc. have
disappeared. Blood biochemical indices, HGB, of ESR, ALB, GLO, ALT, and AST indicators into a normal
number of significant increase (P<0.01 and P<0.05). Results showed that QMG can significantly improve
the CP-induced mouse thymic and splenic atrophy, plus ConA stimulated mouse spleen lymphocyte
proliferation rate increased significantly (P<0.01); increase in thymus, spleen suspension and serum
white interleukin 2 - (of IL-2), of γ-interferon (of IFN-of γ) the secretion (P<0.01); can improve of CD4 +, of
CD8 + cells of the The number and CD4/CD8 ratio (P<0.01 and P<0.05), is capable of regulating the
dynamic equilibrium of the T lymphocyte subsets. Conclusion: QMG honey cream rich in protein,
vitamins and other nutrients, to meet patients' physical needs and disease consumption, to improve the
nutritional status of pulmonary TB patients, reducing the side effects of chemotherapy drugs, improve
immunity and protect the liver and kidney function, so improve the efficacy of adjuvant therapy. The rapid
development of great significance and broad application prospects for the future development of other
natural resources, nutrition and treatment, non-toxic side effects of special nutritional food, to carry out
effective prevention and control of tuberculosis and to speed up the economy of the west.
A-163
Clinical Therapeutic Efficacy and Security Assessment of Uighur medical treatment for
Vitiligo with Uighur Medicine
Tursun Wuphour *1, Yüsup Metenur 1, Silafu Aibai 1*, Ëziz Yasen 1, Shewket abdula 2, Roshen gül
Shawuer 3, Aixirejiang Sidike 3, Erkin Turdi3 ,Qurban Eli 4, Yitaipaer mulate 1, Mubareck Paizula 1,
Huzanur Qurban 1, Yilikaer Baiketiya 1, Julaiti Abduwayiti 1, Zaitungül Wuphour 1, Ekber Anzhaer 1,
Refuhati Saimaiti 1
1 Xinjiang Uighur Autonomous Region Hospital of
Uighur Medicine; 2Kashi Hospital of Uighur Medicine; 3Hotan Hospital of Uighur
Medicine; 4Korla Hospital of Uighur Medicine, China; *Corresponding author: Silafu · AIbai Email: aibai@263.net ;
Tursun·Wuphour Email: tursun122@yahoo.com.cn
Objective: Evaluate the clinical therapeutic efficacy and safety of treating vitiligo with Uighur Medicine.
120
Method: treat leukoderma patients with the Uighur medical treatment, as well as abnormal mucilaginous
maturing agent and scavenger, Zibifu Sugar Liquor, Vitiligo Ointment, Kali Injection, Compound Kali
Tablet, Yi Ti Re Fei Li Ai Ke Mu Ai Li, Vitiligo Liniment, insolation and ultraviolet therapy, and conduct
therapeutic efficacy and safety evaluation before, after and during the treatment. Result: No significant
difference (before the treatment) of white spot total area to the one during the treatment: Z=0.42, P=0.68;
significant difference (before the treatment) of white spot total area to the one after the treatment:
Z=3.91**, P<0.01; significant difference (before the treatment) of pigment regeneration total area to the
one during the treatment: Z=10.2**1, P<0.01; significant difference (before the treatment) of pigment
regeneration total area to the one after the treatment: Z=17.63**, P<0.01. The patients suffer from no
severe safety problem or adverse reaction. Conclusion: Uighur medical treatment with Uighur Medicine
has actual therapeutic efficacy and perfect safety.
A-164
Correlation Analysis of Syndrom Differentiation of Vitiligo and Its Munzich and Mushil
Therapy Based on Traditional Uighur Medicine Theory
Silafu Aibai 1, Tuerxun Wufuer 1*, Aizezi Yasen 1, Abudoujili Abudouaini 1, Zaituguli Wufuer 1, Guzainuer
Kuerban 1, Julaiti Abuduwayiti 1, Aikebaier Anzaer 1, Refuhati Saimaiti 1
1Xinjiang
Uygur Traditional Medicine Hospital, 830049, Urumqi China; The Key Disciplines of Uygur Medical Dermatology of
State Administration of Traditional Chinese Medicine, 830049, Urumqi, China,* Corresponding author: Tuerxun Wufuer
Objective: In this study, 341 cases of vitiligo patients in line with the inclusion creteria were conducted
syndrome differentiation typing analysis on admission. Risk factors, the original Mizaj (temperament) and
abnormal Hilit distribution, factors affecting the efficacy, the top ten symptoms that most intensively
associated with the syndrom differentiation type were analysed. On this basis, quantitative assessment
were conducted on white spots and pigment regeneration area and the symptoms before and after the
use of Munzich and Mushil. And with multivariate statistical analysis, were analysed the factors of
morbidity and induced vitiligo and affecting the efficacy in order to lay the foundation for the
standardization of disease diagnosis of abnormal Hilit while correlation analysis were conducted between
correlation of Munzich and Mushil therapy to restore the vitiligo systemic pathological changes. Results:
Patients with Hol Sohuq Mizaj (wet cold temperament) accounted for 32.8%, with Kuruk Issik Mizaj (dry
and hot temperament) accounted for 29.9% of the, with Hol Issik Mizaj (hot and wet temperament)
accounted for 7.3%, with Kuruk Sohuq Mizaj (dry and cold temperament) accounted for 29.9%. Eating
habits in the risk factors accounted for 95.9%, followed by mental factors as 9.4%, and Moza Temlik
Bilghem(astringency phlegmatic) accounted for 34.6% in healing Hilits. These are consistent with the
premise research project Retrospective Analysis of Uighur Medical Diagnosis of Vitiligo by this research
team. Compared with the time of admission, the white spots mean total area of patients has significantly
narrowed (P <0.01) after the use Munzich and Mushil, leukoplakia district obvious pigment regeneration
(P <0.01), symptoms improved significantly ((P <0.01). Conclusion: Due to the incentives such as eating
habits and mental factors,patients with Kuruk Sohuq Mizaj (dry and cold temperament) and Hol Sohuq
Mizaj (wet cold temperament) phlegm in the body gradually increases and goes beyond the body's
self-regulating capacity led to vitiligo. The Moza Temlik Bilghem (astringency phlegmatic) vitiligo is
relatively common according to the original temperament reasons.This matches one of the typical Uygur
Medicine classic "Mehzanul Edwiye" records: Moza Temlik Bilghem (the astringency phlegm) at the same
time has the characteristics of excessive humidity and excessive cold, one of typical abnormal Hilit, so
that vitiligo is most common.Munzich and Mushil Therapy mainly through mature and remove abnormal
Belghem Hilit (phlegm) and regulate the body environment, strongthen natural forces to control the
disease progresses. Avicenna, a famous ancient doctor in the 11th century, in his book Medical Code,
and Muhammad Akbarali in 1750 AD in his book Tibbi Akbar recorded: Vitiligo is caused by strong
121
concentrated phlegm,by which the transformation force and the exclusion force of the body weakened,so
that the metabolic productions of the body cannot be excreted properly, and cause the vitiligo lesion to
occur. Munzich and Mushil therapy is to correct abnormal temperament, to promote the generation of
normal Hilits（
body fluids）
and restore the body's natural forces to control the proliferation of leukoplakia.
The result of this research is consistant with the records. The results of this research proved the above
points of ancient scholars from the point of view of modern medicine combined with Traditional Uighur
Medicine theory.
A-165
Detection of neuroendocrine and immune factors before and after traditional Uighur
medicine treatment of vitiligo and it’s significance
Aikebaier Anzhaer 1, Silafu Aibai 1*, Tuerxun Wufuer 1, A.Aimaiti 1, Aizezi Yasen 1, G.Kuerban 2,
J.Abuduwayiti 2, A.Haibibula 2
1
Uyghur Medicine Hospital of Xinjiang Uighur Autonomous Region , 830049, Urumqi, China; 2Uighur medicine skin disease key
subjects of State Administration of Traditional Chinese Medicine, 8300493, Urumqi, China; Uighur medicine vitiligo diagnosis
and treatment key unit of State Administration of Traditional Chinese Medicine, 830049, Urumqi , China, *Corresponding author:
Sirap.Abai, Email:aibai@263.net
Objective: Explore the relationship between vitiligo and the development of neuroendocrine-immune
network‘s environmental change of patients, combined with the Uighur medical theory, explore its
relationship with vitiligo. Methods: Before and after receiving Uighur medicine’s treatment, 200 Vitiligo
patient’s blood serum are assayed for IFN-γ，sICAM-1，5-HT，IgG、Cortisol， NE，ACTH，IL-10，
MT，EP-β，CRH and E level by enzyme linked immunosorbent assay (ELISA). Results:（1）Following
index :IFN-γ、NE、ACTH、IgG、5-HT、IL-10、sICAM-1、EP-β、E, have significant difference compared
with those before treatment. IFN-γ、NE、ACTH、5-HT、IL-10、EP-β、E are tends to normal control
level. Conclusion: Those results indicates that the invasion of Vitiligo is a comprehensive course of
neuroendocrine-immune network, which corresponds with the Uighur medicine’s theory that imbalanced
“kuwiti tabiiy” [“Nature power”(of human body)] induces Vitiligo. Traditional Uighur medical treatments
can improve the environment within the neuroendocrine-immune network of patients with vitiligo.
A-166
Discussion on the relationship between vitiligo and peripheral blood in traditional Uighur
Medicine
R.Umar 1, J.Abduwayet 2, T.Tursun 3
1Xinjiang
Uighur autonum region Uighur medicine hospital, 830049 ,Urumqi, Xinjiang Uighur Autonomous Region，China;
2Xinjiang
Uighur autonum region Uighur medicine hospital, 830049 ,Urumqi, Xinjiang Uighur Autonomous Region，China;
3Xinjiang
medical university traditional Uighur medicine institute, 830054, Xinjiang Uighur Autonomous Region，Urumqi, China
Objective: Discussion on the relationship between vitiligo and peripheral blood. Methods: 100 cases of
skin clinic, hospital vitiligo patients and In 100 patients the hospital health care branch to attend the
examination of normal people were peripheral blood detected (Red blood cells and its classification、
white
blood cell, and its classification). Result: And compared with the normal group and patients group with
vitiligo RBC、
HGB、
HCT、
MCV、
MCH、
MCHC reduce (P<0.05), RDW-SD rise (P<0.05). Conclusion:
Compared with normal subjects, patients with vitiligo have differences in peripheral blood, the red cells
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and its classification is obviously different.
A-167
Effect of Euphorbia humifusa active component on the cell membrane biosynthesis and
gene expression of metalloprotease(MEP), subtilisin(SUB) of Trichophyton rubrum
Li Zhijian 1,2, Zhao Ming yue 1, An Huixia 1, Gulnar Dawuti 3, Silafu Aibai 1,2,3*
1Xinjiang Institute of Traditional
2Key Laboratory
Uighur Medicine, Urumqi 830049, Xinjiang Uighur Autonomous Region，China；
3Uighur Medicine
of Traditional Uighur Medicine Prescription, Urumqi 830049, Xinjiang Uighur Autonomous Region，China；
Hospital of Xinjiang Autonomous Region, Urumqi 830049, China, *Corresponding author: Silafu Aibai, ,Professor, Doctoral
supervisor, Email:aibai@263.net. Author:Lizhijian, Research Assistant, Email:lizhijian0220@sina.com.
Objective: To study the effect of Euphorbia humifusa active component(EHAC) on CYP51,lanosterol
biosynthesis, ergosterol biosynthesis and the expression of gene metalloproteinases(MEP) and
subtilisins(SUB) in Trichophyton rubrum, and explore the potential mechanism of their antifungal activity.
Methods: Treated T.rubrum with EHAC, the effects of EHAC on CYP51 studied by ELISA test, the
content of lanosterol and ergosterol in mycelium of Trichophyton rubrum detected by reversed phase high
performance liquid chromatography, and total RNA of Trichophyton rubrum was extracted for
semi-quantitative RT-PCR to detect the expression of MEP and SUB．
Results: EHAC with 32 μg/ml could
inhibit CYP51 activity and the expression of gene MEP and SUB in Trichophyton rubrum (p<0.01),and
accumulate lanosterol biosynthesis and decrease the content of ergosterol (p<0.01) with a
dose-dependent manner during 32～
128 μg/ml in dermatophyte. Conclusion: EHAC can inhibit CYP51
enzyme activity in Trichophyton rubrum, leading to accumulation of lanosterol and inhibit on ergosterol
biosynthesis, and inhibit dermatophyte cell membrane biosynthesis thereby. Also, EHAC can inhibit the
expression of MEP and SUB in dermatophyte, influent getting nutrition during infection process and
narrowing invasion.
A-168
Effect of Uighur Medicine Maizhuni on the Expression of MMP-1, MMP-3, MMP-13, TIMP-1,
IL-1β, and Aggrecan in Rat Osteoarthritis Models
Zhang Yaowu1, Meng Qingcai1,2*
1Affilated
Traditional Chinese Medicine Hospital, 2Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi
830011, Xinjiang Uighur Autonomous Region，China; *Correspondence should be addressed to mengqincai@medmail.com
Objective: To establishe the OA model, To investigate the expression of matrix metalloproteinase1, 3,
13, Interleukin-1βand tissue inhibitor of matrix metalloproteinase 1 in articular cartilage and the
intervention effects of Uighur medicine Maizhuni. Methods: A total of 40 healthy male SD rats were
randomly divided into Maizhuni group and model group，
shamed operation group and normal control
group. Rats were treated with Uighur medicine Maizhuni or saline by gavage in the 2nd week after model
construction for 4 weeks. Results: The model of OA animal model was successfully established.
Compared with model group, the degree of rat knee joint cartilage degeneration in Maizhuni group was
alleviated, The cartilage general score and Mankin score in Maizhuni group was lower than that in the
model group (P < 0.05), The score of Maizhuni group was higher than in the shamed operation group
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and normal control group(P < 0.05)，
there are no differece beteeen normal control group and shamed
operation group(P>0.05) ,The expression of matrix metalloproteinase1,3,13 in knee joint chondrocytes of
Maizhuni group decreased than that of the control group(P < 0.05)；
The expression of interleukin1βin knee
joint chondrocytes of Maizhuni group also decreased than that of the control group(P < 0.05);The
expression of Aggrecan in knee joint chondrocytes of Maizhuni group increased than that of the control
group;The expression of tissue inhibitor of matrix metalloproteinase 1 in Maizhuni group increased than
that in the model group (P < 0.05). Conclusion: These findings indicate that Uighur medicine Maizhuni
may protect articular cartilage by down-regulating the expression of matrix metalloProteinase 1,
up-regulating the expression of tissue inhibitor of matrix metalloproteinase 1, reduce the expression of
interleukin-1β.
A-169
Research of Quality Control about Abnormal Savda Munziq Granular andα-Glucosidase
Inhibitors
Xin Lude 1, Tian Shuge1, Halmurat Upur2*
1
Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region，,
China; 2Traditional Uygur Medicine Institute, Xinjiang Medical University, 393 Medical University Road, 830011, Urumqi , Xinjiang
Uighur Autonomous Region，China；* Correspondence should be addressed to Halmurat@263.net
Objective: The qualitative and quantitative study of main active ingredient in Abnormal Savda Munziq
Granular and the research of α-glucosidase inhibition, It Provides a stable and reliable method for the
quality control of Abnormal Savda Munziq Granular, providing corresponding research data for Abnormal
Savda Munziq Granular’s clinical application and development. Method: Using thin-layer
chromatography (TLC) screening and qualitativethe main active ingredient of the Abnormal Savda
Munziq Granular; Using the high performance thin layer chromatography (HPTLC) method for the
determination of the content of rutin, rosemary acid, glycyrrhizic acid, Liquiritin; Using high performance
liquid chromatography method (HPLC)for the determination of rutin, using High Resolution Rapid
Resolution Liquid chromatography method RRLC determines the content of rosmarinic acid, glycyrrhizic
acid, liquiritin; using the part of the extract of the four Abnormal Savda Munziq Granular, which is
Petroleum ether, ethyl acetate, ethanol, water has a test on α-glucosidase inhibitory activity and
examined by Bio-luminescence photometer. Results: Using ultrasonic extraction, determines Rosmarinic
acid and rutin in Abnormal Savda Munziq Granular qualitatively and quantitatively with ethyl acetate formic acid - acetic acid-water (15:1:1:1.5) as developing solvent; ethyl acetateacetate - formic acid-acetic
acid-water(15:1:1:1.7) as the developing solvent determines glycyrrhizic acid and liquiritin in
Abnormal Savda Munziq Granular qualitatively and quantitatively , The test sample with the standard, or
control medicine’s chromatography spots have the same color and Rf values, Negative control has no
significant interference,there are no large differences about rosmarinic acid, rutin, licorice glycoside,
glycyrrhizic acid’squantitative results with Liquid chromatography; water part of the extract of Abnormal
black Munziq has a good α-glucosidase inhibitory activity, Petroleum ether extract part has no inhibitory
activity. Conclusion: The topic using TLC, HPTLC, HPLC, RRLC determines Rosmarinic acid, rutin,
licorice glycoside, glycyrrhizic acid in Abnormal black Munziq qualitatively and quantitatively, using
bio-luminescence photometer determines four extraction sites of Abnormal black Munziq ‘s inhibition for
Α-glucosidase enzyme activity, The method specificity, simple, accurate, can be used for the Abnormal
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black Munziq’s qualitative and quantitative identification , also provides a reliable method for quality
control of the abnormal black Munziq.
A-170
Retrospective Survey and Analysis on Ulcerative colitis Treated with Uighur Medicine
Turgun Amar 1, Jurat Tohti 2, Sirap Abai 3*, Ahmatjan Mamat 4, Yusup Matnur 5, Ruxangul Sawur 6, Xawkat
Abdulla 7, Ubul Asan 8, Kurban Ali 9
1,2,8Xinjiang
Uighur medical school and it’s affiliated hospital, 848000, Hetian, Xinjiang Uighur Autonomous Region，China; 3,4,5
Uighur medical hospital of Xinjiang Autonomous region, 830049, Urumqi, Xinjiang Uighur Autonomous Region，China; 6Hotan
Uighur medical hospital , 848000, Hetian, Xinjiang Uighur Autonomous Region， China ;7Kashghar Uighur medical hospital,
844000, Kashi, Xinjiang Uighur Autonomous Region，China,9Kurle Uighur medical hospital, 841000, Kuerle, Xinjiang Uighur
Autonomous Region，China, *Corresponding author: Sirap.Abai, Email:aibai@263.net
Objective：Review the 10 years of Ulcerative colitis clinical materials of five Uighur medical hospitals in
Xinjiang, summarized and analyzed it’s distribution in curative methods and principles, drug type, drug
use principles, non-drug treatment, Efficacy judgment and nursing measures etc. Methods：Retrieve
the 180 Ulcerative colitis cases which hospitalized by the Uighur medical hospital of Xinjiang
Autonomous region, a total of 5 Uighur Medicine Hospital since 1998 to 2008, conduct a retrospective
study and analysis on the therapeutic, nursing materials and conclude the clinical data of these cases .
Results：Among all, 180 cases reach into the standard, so conduct a classified statistics on their
treatment process. Among all, 40 cases recovered (22.22%), 38 cases effectual(21.11%),67 cases
vali(37.22%), 29 cases invalid (16.11%), 6 cases in stable condition (3.34%). Conclusion：Simply occur
in patients with ulcerative colitis, as have different curing, nursing measures and food taboos，which
expresses traditional factors of individualized therapies. But the hospital examination conditions,
treatment of blind and without Uighur medicine for the treatment of serious phenomenon. According to
patients with a history of length, they appear different clinical curative effect.
A-171
Retrospective Survey, Research and Analysis on Osteoarthritis of the Knee treated with
Uighur medicine
Jurat Tohti 1, Turgun Amar 2, Sirap Abai 3*, Ahmatjan Mamat 4, Yusup Matnur 5, Ruxangul Sawur 6,
Xawkat Abdulla 7, Ubul Asan 8, Kurban Ali 9
1,2,8Xinjiang
Uighur medical school and it’s affiliated hospital, 848000, Hetian, Xinjiang Uighur Autonomous Region，China; 3,4,5
Uighur medical hospital of Xinjiang Autonomous region, 830049, Urumqi, Xinjiang Uighur Autonomous Region，China; 6Hotan
Uighur medical hospital , 848000, Hetian, Xinjiang Uighur Autonomous Region，China ;7Kashghar Uighur medical hospital,
844000, Kashi, Xinjiang Uighur Autonomous Region，China,9Kurle Uighur medical hospital, 841000, Kuerle, Xinjiang Uighur
Autonomous Region，China, *Corresponding author: Sirap.Abai, Email:aibai@263.net
Objective: Review the 10 years of Osteoarthritis of the knee diagnosed materials of five Uighur medical
hospitals in Xinjiang, summarize and analyze the treatment methods and principles of Osteoarthritis of the
Knee, and the methods and principles of Traditional Uighur Medicine. Methods: Retrieve the 500
125
Osteoarthritis of the Knee cases which diagnosed by the Uighur medical hospital of Xinjiang Autonomous
Region, the affiliated hospital of Xinjiang Uighur Medical School, the Uighur medical hospitals in Hotan,
Kashghar and kurle provinces since 1998 to 2007, conduct a retrospective study and analysis of the 500
cases on the therapeutic, nursing and treatment evaluating materials and conclude the clinical data of
these cases. Results: Among all 500 cases 410 cases reached into the standard upon conducting a
classified statistics on their diagnosed process with Traditional Uighur Medicine, nursing and treatment
evaluating methods. By screening the 410 standard cases, 269 cases (65.61%) have discharge
diagnosis, and there is no discharge diagnosis in the medical records of the other 141 cases (34.39%).
Among those 269 cases with therapeutic evaluation 4 cases (0.98%) were cured, 136 cases (33.17%),
were significantly effective, 121 cases (29.51%) were effective, 6 cases (1.46%) were not effective, 2
cases (0.49%) were in stable condition. Conclusion: As the causes of Osteoarthritis of the Knee the Hilit
Ghayri Tabiyi(Abnormal body fluids) and the mizaj(temperament) of the patients can differ. Thus different
curing, nursing measures and diet prohibits measures are required, which expresses traditional factors of
individualized therapies. The effectiveness of the treatment differs according to the length of the disease,
the differences of the Hilit type as causing factor, and differences in principles and methods while treating.
The proportion of significantly effective and the effects are higher in this research.
A-172
Studies on Anti-Aphthous Ulcer and Mechanisms of Galla Turcica Extract treating
Recurrent Aphthous Ulcer in experimental animals
Fan XiaoHong 1, Li Zhijian 1,2, Silafu Aibai 1,2,3*
1Xinjiang Institute of Traditional Uighur Medicine, Urumqi 830049,
2Key Laboratory
Xinjiang Uighur Autonomous Region， China；
3Uighur Medicine
of Traditional Uighur Medicine Prescription, Urumqi 830049, Xinjiang Uighur Autonomous Region，China；
Hospital of Xinjiang Autonomous Region, Urumqi 830049, Xinjiang Uighur Autonomous Region，China; *Corresponding author:
Silafu Aibai, Professor, Email:aibai@263.net.
Objective: Observe the effect of Galla Turcica extract(GTE) on oral ulcer and initially investigate its
possible mechanism. Methods: Models were duplicated through phenol cauterization in oral cavity. Oral
mucosa protein and other matter extracted from experimental rabbits were applied to immue rats as
antigen，
Antigen above added adjuvant was injected to intraderm adjacent the rats spine once per two
weeks to observe the occurrence and cicatrization of oral ulcer in the experimental rats, Anti oral mucosa
antibody in rats bled serum was detected, and histopathology examination was performed with oral ulcer
mucosa. To study the anti-inflammatory mechanism of GTE, ulcer area, congestion and recovering time,
Activity SOD、
MDA in rats bled serum was detected. The effect of the concentration of GTE on cell viability
was evaluated using MTT and Trypan blue. Investigated on macrophage cells (RAW264.7) were
determined by using neutral red assay. The supernatant was transferred into 96-well ELISA plate and
then TNF-α concentrations were determined using commercial ELISA kits according to the
manufacturer’s instruction. RT-PCR analysis for COX-2、
TNF-α and iNOS mRNA expression. Results:
GTE relieved mice body writhing response induce by ethanoicacid, Compared with the normal control
group (P<0.01). GTE decreased ulcer area(p<0.05), relieved congestion (p<0.05)and in rat model
induced with phenol.All of the experimental rats developed oral ulcer with oral mucosa protein as antigen,
which were anti oral mucosa antibody positive in rats blood serums. There was the significantly difference
between oral mucosa protein group and deposit group (p<0.01),it also decreased recovering time
126
(p<0.05). GTE could inhibit the mouse ear edema induced by xylene, inhibit permeability of capillaries in
abdomen of mouse due to acetic acid, inhibit the rat swelling induced by carrageenin, inhibit cotton ball
granuloma of rat. GTE markedly inhibited TNF-α release. It also suppressed mRNA expression of
iNOS、
COX-2 and TNF-α in dose-dependent manners. Conclusion: GTE can promote the healing of oral
ulcers in rats, reduce the incidence of oral ulcers immunity, reduce the local inflammatory response,
enhance immune function and play a role of analgesia in mouth. So we speculate that the GTE may treat
the oral ulcers by the combined effects of all of the above channels. Besides, the GTE can inhibit LPS
stimulating the expression of the RAW264.7 cells’ pro-inflammatory cytokines, which may be one of
anti-inflammatory mechanisms of action of GTE.
A-173
Study of in vivo - in vitro Anti- Influenza virus and Anti-inflammation and immune function
of Artemisia rupestris extract
Meng Fanlong 1, Li Zhijian 1,2, Silafu Aibai 1,2,3*
1Xinjiang
Institute of Traditional Uighur Medicine, Urumqi 830049, China; 2Key Laboratory of Traditional Uighur Medicine
Prescription, Urumqi 830049, China; 3Uighur Medicine Hospital of Xinjiang Autonomous Region, Urumqi 830049, China;
* Corresponding author: Silafu Aibai, Uighur, Professor, Doctoral supervisor, Email:aibai@263.net.
Objective: Study of in vivo - in vitro Anti- Influenza virus and Anti-inflammation and immune function of
Artemisia rupestris extract(AR). Methods: The experiment use a method that AR at the different dose add
into the cells be infected by influenza virus, to observe and note the cytopathic effect (CPE) by inverted
microscope and calculate the EC50,to evaluate the effect of AR against kinds of virus. We used the model
that mouse be infected by influenza virus type A(FM1 strain) to observe anti-influenza virus effect of AR in
vivo, used inflammatory models with xylene-induced mouse ear swelling test, increased of permeability of
capillaries in abdomen of mouse due to acetic acid, injected carrageenin into the toe of rats, cotton ball
granuloma of rats to study the effect of AR on aute and chronic inflammation, and used immune models
with delayed hypersensitivity test, phagocyte swallow neutral red test, T,B lymphocytes increasing test,
the production of serum hemolysin of mouse to study the effect of AR on immune reaction. Results: The
EC50 of AR on H3N2, H1N1, BV39 were 143.14 μg/mL,333.33 μg/mL,111.11 μg/mL. AR under the 300
mg﹒
kg-1,the lung index and lung index inhibition ratio of AR on FM1 strain infect mouse respectively are
1.20±0.35、
48.72%,compared with model control group,AR have the significant difference. AR could
inhibit the mouse ear edema induced by xylene, inhibit permeability of capillaries in abdomen of mouse
due to acetic acid, inhibit the rat swelling induced by carrageenin, inhibit cotton ball granuloma of
rats,decrease inflammatory exudate PGE2. AR could not inhibit the delayed hypersensitivity induced by
DNCB,but could increase the index of thymus and spleen.AR could increase the number of T,B
lymphopiesis,increase the production of serum hemolysin of mouse,activate macrophages’phagocytosis.
Conclusions: AR have obvious effects on influenza virus in vitro and in vivo. It could increase the number
of T,B lymphocyte,increase the production of serum hemolysin of mouse, activate phagocytosis of
macrophage, inhibit acute inflammation, subacute inflammation,chronic inflammation.
A-174
The Nymphaea alba with oxidative damage protection to human umbilical vein
endothelial cells
Lan Wei1, Halmurat Upur1*
127
1Xinjiang
Key Laboratory of Famous Prescription and Science of Formulas, Urumqi, 830011, China;1*Corresponding authors:
Halmurat Upur, Traditional Uighur Medicine Department, Xinjiang Medical University, 830054 Urumqi, Xinjiang Uighur
Autonomous Region，China, halmurat@263.net; tel +86-991-4362206 Fax: +86-991-4361554
Objective: Screening Nymphaea alba with oxidative damage protection to human umbilical vein
endothelial cells. Methods: With the concentration of 50 μg • mL-1 sample for 4 h to cells, then using
H2O2 treated it for 20 h to cause the cells to oxidative damage model, using MTT method to determine
the activity of the mitochondria vascular endothelial cells in order to observe samples of H2O2 endothelial
cells of oxidative damage caused by the protection. Results: The Nymphaea alba extractings protection
rate is 58.58%, and the EC50 is 19.22μg • mL-1, indicating that the Nymphaea alba extractings have
protection to human umbilical vein endothelial cells oxidative damage. Conclusion: This study show the
Nymphaea alba can be used for future cardiovascular new drug development to provide the evidence.
A-175
Uighur Medicine Capparis spinosa treatment of 67 Cases of chronic arthritis
Hupurjan Obul1
1The
second poor hospital of Xinjiang Uighur Autonomous Region, 830011,Urumqi，Xinjiang, China
Objective: To observe the efficacy of the treatment of chronic arthritis Uighur Medicine Capparis spinosa.
Methods: 92 cases of chronic arthritis patients were divided into two groups, namely, treatment and
control groups. For the treatment group, there were 67 cases of chronic arthritis patients, according to the
cause of abnormal Hilit Munziq agents, using painkillers, anti-inflammatory drugs, swelling of the drugs
and drugs to improve joint function, and using the Capparis spinosa medicinal bath therapy to achieve
better effect. For the control group, there were 25 cases of chronic arthritis patients, according to the
cause of abnormal Hilit, using Munziq agents, mature agent, analgesics, anti-inflammatory drugs,
swelling of the drugs and drugs to improve joint function. Results: 67 cases of patients with chronic
arthritis patients cured, 34 cases, accounting for 51%, a significant effect of 22 cases, accounting for 33%,
effective 8 cases, accounting for 12%, 3 cases (4%), the efficacy of 96%. For the control group, 25 cases
of chronic arthritis patients, 10 cases recovered, accounting for 40%, a significant effect of 7 cases
accounted for 28%, effective four cases, accounting for 16%, 4 cases, accounting for 16%, 84% efficacy.
The difference between the two groups was statistically significant (p <0.01).Conclusion: The treatment
of chronic arthritis, according to the cause of the abnormal Hilit Munziq agent and use of painkillers,
anti-inflammatory drugs, swelling of the drugs and drugs to improve joint function, after using the Capparis
spinosa medicinal bath therapy has better effect than in the control treatment group, and the treatment
time is shorter with lower costs.
A-176
Uighur Medicine Karapxa decoction as a hypouricemic agent: reduces serum uric acid
level, inhibits liver xanthine oxidase in hyperuricemic mice model and, scavenge free
radicals in vitro
Nurmuhammat Amat1, Palidan Wushuer2, Halmurat Upur1*
128
1Traditional
Uighur Medicine Department, Xinjiang Medical University, Urumqi-830011, Xinjiang Uighur Autonomous Region，,
PR China ;2Neurology Department, Xinjiang Medical University Affiliated Traditional Chinese Medicine Hospital,, Urumqi-830000,
Xinjiang Uighur Autonomous Region, PR China; *Corresponding author: Traditional Uighur Medicine Department, Xinjiang
Medical University, 393 Medical University Road, Urumqi, 830011 , Xinjiang Uighur Autonomous Region，PR China; Tel.: +86
991 4362206 fax: +86 991 436 ，
E-mail: halmurat@263.net
Karapxa decoction, is a traditional Uighur medicines formula for hepatitis, cholecystitis，
gastralgia, gout,
and arthralgia, anti-edema prescription in Traditional Uighur Medical literatures and State Uighur
Medicine Pharmacopoeia of People’s Republic of China. Karapxa decoction is one of the most frequently
prescriptions in Traditional Chinese Medicine to treat gout and hyperuricemia. In the present study, we
investigated the water extracts of Karapxa decoction (KD) at 200，
400 and 800mg/kg for 14 days were
demonstrated to possess in vivo potent hypouricemic effects in both hyperuricemic mice induced by yeast
extract paste and potassium oxonate respectively, and standard drug allopurinol (10mg/kg) was given as
a positive control. Scavenging activity of KD on 1, 1-diphenyl-2-picrylhydrazyl radicals (DPPH), nitric
oxide (NO), superoxide (O2.), efficiency against lipid peroxidation, and xanthine oxidase (XO) inhibitory
effects were determined in vitro. Serum levels of uric acid, mouse liver XO activities were measured in
hyperuricemic mice treated with KD and allopurinol. The results demonstrated potent hypouricemic
effects both in hyperuricemic mice, KD can reduce UA and inhibit the liver XO activity. In addition, The KD
extract showed noticeable antioxidant activity, through the ability to scavenge free radicals (DPPH, NO
and O2.) and efficiency against lipid peroxidation, as well as has inhibitory effects on XO activities in vitro.
This function could make Karapxa decoction a potentially valuable drug for the treatment of hyperuricemia
and gout, and effects may be achieved by other mechanisms in part because of XO inhibition and
antioxidant function.
A-177
Kali Zira （
Vernonia anthelmintica Willd ）
injection induced allergic reaction in 6 cases
R.Umar 1, J.Abduwayet 2, Tursun Wuphour 3
1
Uighur Medicine Hospital of Xinjiang Uighur Autonomous Region, 830049, Urumqi, China;
Xinjiang Uighur Autonomous Region, 830049 ,Urumqi, China;
3
2
Uighur Medicine Hospital of
Traditional Uighur Medicine Institute of Xinjiang Medical
University, 830054, Urumqi, China
The Traditional Uighur Medicine injection Kali Zira injection is composed of Vernonia anthelmintica Willd
extraction. It has long been used in Traditional Uighur Medicine for treat vitiligo. According to the
traditional Uighur Medicine theory KD was described to treat the diseases through balancing the Mizaj in
the liver, eliminating the Abnormal Balgham Hilit (damp and cold)，
excreting dampness. Kali Zira injection
is recorded in State Pharmacopoeia of People’s Republic of China in the Uighur Medicine volume. Kali
Zira injection injection for the treatment of vitiligo with classic prescription of Uighur Medicine. It is capable
of regulating abnormal balgham, promoting blood circulation and coloring, increasing melanin cell
function. This paper were reviewed the drug adverse reactions in recent years. We found 6 patients has
side effect or allergic reaction.
A-178
Uighur Medicine Xipayi Kui Jie’an effects Gene Expression Profiles on Model of
129
ulcerative colitis rat colon organization
Yunusi Ki1，
Z.Jingping 1，
Y.Mijit 1，
M.Hongchao 1，
Y.Mei 1，
Xu Chenbo 1, Halmurat Upur 2*
1Basic
Medical Institute of Xinjiang Medical University, Xinyi Road 393, Xinjiang Uighur Autonomous Region， 830011,PR
China;2Traditional
Uighur Medicine Institute of Xinjiang Medical University, Xinyi Road 393, 830011, Xinjiang Uighur
Autonomous Region，PR China;*Corresponding author. Corresponding author. Halmurat.Upur email:halmurat@263.net
Objective: Apply the of transcriptomics technologies for the detection of ulcerative colitis ( UC ) in the
rat colon tissue of the level of structure gene expression, screening of differentially expressed genes,
and their correlation analysis, to explore the pathogenesis of UC and Uighur Medicine Xipayi kuijiean
(KJA) mechanisms of action.Methods: Wistar rats were randomly divided into normal group, model
group, using 2,4-dinitrochlorobenzene( DNCB ) and acetic acid compound induced rat model of UC. UC
rats were randomly divided into model group, normal saline (NS) treated group as negative control, KJA
interfered group (large-dosage group, middle-dosage group and small-dosage group), and drug
intervention for 20 days. Application of chip27K Rat GenomeArray chip detection of the level of rats
colonic tissue structure gene expression, screening of differential expression gene and bioinformatics
analysis. Using real-time quantitative fluorescence PCR technique on the part of differentially expressed
genes were further verified. Results: The rats in the model group of symptoms, signs, colonic tissue
specimens, pathological findings were consistent with UC model standard; compared with the normal
group, model group have 1054 differentially expressed genes, in which the expression of 667 genes
were up-regulated, 387 genes were down-regulated. Compared with normal saline negative control
group, different doses of the drug intervention group in the middle of 667 up-regulated genes have 414
gene expression were down-regulated, in the expression of 387 genes down-regulated have 168
up-regulation of gene expression. Differential gene expression related with intercellular signaling,
metabolism, immunity, inflammation and other life activities, including TLR2, IL-1 β, NF-κB and TGF-β1
genes using real-time quantitative fluorescence PCR technique of mRNA level of verification, the results
show real time fluorescent quantitative PCR findings with microarray results have a good consistency.
Conclusion: We concluded that the pathogenesis and KJA therapeutic effect of UC rats were
associated with changes of gene expression profile. On differential gene expression analysis will
contribute to further elucidate the mechanism of UC and KJA mechanism of action, and provide novel
therapeutic targets of UC.
A-179
Uighur Medicine Xipayi Kui Jie’an effects appreciation and apoptosis on HT-29 human
colon cancer cells
K.Yunusi 1，
Y.Mijit 1，
Z.JingPing 1，
L.JingJing 1, Halmurat Upur 2*
1 Basic
Medical Institute of Xinjiang Medical University, Xinyi Road 393, 830011, Xinjiang Uighur Autonomous Region，PR
China;2Traditional
Uighur Medicine Department of Xinjiang Medical University, Xinyi Road 393, 830011,PR Xinjiang Uighur
Autonomous Region，China;*Corresponding author. Corresponding author. Halmurat.Upur email:halmurat@263.net
Objective: To investigate the Uighur Medicine Xipayi kuijiean (KJA) effects appreciation and apoptosis
on HT-29 human colon cancer cells, in order to prevent and cure ulcerative colitis tissue cell
carcinogenesis to provide experimental basis. Methods:Using four methyl thiazolyl tetrazolium (MTT)
130
method to determine the proliferation inhibition rate of various concentrations KJA in human colon
cancer HT-29 cell, flow cytometry technique was used for cell apoptosis. The application of different
concentrations of KJA intervention in human colon cancer HT-29 cells at the time of
3h,6h,12h,24h,48h,72h, observed influence of the cell proliferation and apoptosis that KJA on HT-29
human colon cancer cell. Results: KJA intervention in human colon cancer HT-29 cells, the decrease in
the number of live cells, cell proliferation was inhibited. Along with the extension of time, the same
concentration of KJA in human colon carcinoma HT-29 cell inhibition rate increased, the difference was
statistically significant (P<0.05); the same time, with the increase of concentration, KJA raise the cell
proliferation inhibition function, the difference was statistically significant (P<0.05), different
concentrations of group differences were statistically significant ( P< 0.05). KJA induced apoptosis in
human colon cancer HT-29 cells effect was concentration, time dependent. Conclusion: KJA in human
colon carcinoma HT-29 cell has certain inhibition of proliferation, apoptosis effect, so the KJA may be
used as candidates drug to prevent ulcerative colitis tissue cancerous.
A-180
Concept, classification and Analysis of Uighur medical toxic drugs in Traditional Uighur
Medicine Hospital of Xinjiang Uighur Autonomous Region
A.Turdi1*
1Traditional
Uighur Medicine Hospital of Xinjiang Uighur Autonomous Region, Urumqi, Xinjiang 830001; *Corresponding
authors: Alip Turdi, E-mail: Alip Turdit@263.net
Uighur Medicine is scientific summary and wisdom of hardworking Uighur people’s activity about fighting
against with diseases over the long-term production practice. It has 2,500 years of history, a complete
theoretical system, abundant practical experience and unique methods of diagnosis and treatment. So,
it is a gem in the treasure-house of Chinese traditional medicine. It is mainly based on Uighur Medicine
theory system to study the basic theory of Uighur Medicine, and is a series of relevant knowledge about
practical experience, drug potency, Drug properties, function, main treatment, usage, dosage, note,
Corrective medicine, Alternative medicine and origin, harvest, as well as the processing. It takes the
most significant part of the Uighur Medicine. Xinjiang Uighur Autonomous Region covers about onesixth of China's total territory, it is our largest and resource-rich province. Resources of Uighur Medicine
are also extremely rich. Since the founding of the People's Republic of China, there have been not only
more systematic understanding about the types of resources of Uighur Medicine, distribution,
conservation, and ecological environment , but there have Also been discovered a new drug source by
making more and more investigation and study on Uighur Medicine resources. According to preliminary
statistics on Varieties of Uighur Medicine, now there is 1100 kinds’ variety: More than 1000 kinds of
Botanicals, 80 kinds of Mineral drugs and 50kinds of Animal Drugs. They are mainly distributed in the
north and south of the Tianshan: Oasis, grasslands, deserts, rivers and lakes of the Tarim and Junggar
Basins. Tianshan Mountains and the hills and forest of the Pamir Also are all the resources of Uighur
Medicine distribution. The source of Uighur Medicine was divided into three categories: Plants, animals
and minerals. Botanicals can be divided into flowers, leaves, bark, roots, juice, fruit, oil, etc. Animal
Drugs can be divided into hair, horn, milk, oil, dung, hoof, blood, eggs, skin, bone marrow, etc. Mineral
drugs can be divided into salts, stone, metals, precious stones, oil, soil, etc. With the development of
medicine, the way for classifying Uighur Medicine is improving continuously. Initially, medicine was
131
classified to with poison and no poison; there was also a way that classifies medicine according to
treatment. Deferent way of classification has its different characteristics. The concept of toxic medicine
in Uighur Medicine is the medicine that has direct harm to Kuwat (three power :spirit, vitality, Natural
force) or can damage as the starting point for three power of Control organ’s: (brain, heart, liver)
function, and can directly bring great threat to vitality and can make body sick. The medicine which has
beyond level three or four of Medicinal properties can be regarded as toxic drugs.
A-181
Clinical effect observation on treatment Adenomyosis with Traditional Uighur Medicine
M.Abliz 1
1Traditional
Uighur Medicine Hospital of Xinjiang Uighur Autonomous Region, Urumqi, Xinjiang 830049, China
Objective: To explore the effect of Traditional Uighur Medicine for treatment adenomyosis.
Methods: 48 cases of adenomyosis were treated with Traditional Uighur Medicine for three
months, the symptom and signs, the follow-up, B-Ultra sonic were reexamined. Results: After
for three month treatment total effective of 40 cases was 87.5%, cure rate was 29.2%,
significant efficiency 62.5%, After the treatment, the symptoms improved obviously and that of
the treatment group was far more obvious, significantly relief, dysmenorrhea significantly
decrease menstrual quantity, Endometrial thickness obvious become thin, in the cured patients
uterine, volume and placed former comparison, significant changes. Conclusion: Traditional
Uighur Medicine therapy for treatment adenomyos is can control condition development
improve clinical symptom.
A-182
Clinical report of the Mongolian psychosomatic medicine interaction therapy for the
treatment of complex disease
Na Gong Bilige1，A Runa1，Chang Ming1，Bao Yin De Li Ge Er1，Chen Fu Yu1, Cao Yao Le Tu1
1Inner
Mongolia International Mongolian hospital, Hohhot University of East Road No. 83, 010016, Hohhot, China
132
Mongolian medicine thinks that man is composed of body and spirit, and the life activities of an whole
organic system runs both at its independent and unified mechanisms, in which the body is the material
basis of mental activities, and the spirit is the life activities power of the body. The body is composed of
three element energies and seven substances, in which the seven substances are the basic material of
human body and the three element energies are the body's life-power. If the body is healthy, the three
element energies maintain a balance, and imbalance will cause diseases.
A-183
On the Mongolian understanding and dialectical treatment of pigmented purpuric
dermatoses
Chen Shana1
1Inner
Mongolia International Mongolian Hospital Hematology, the Hohhot Saihan University of East Road No. 83, 010065,
Hohhot, China
Pigmented purpuric dermatoses is a common group disease clinically. The main characteristic of it is
capillary inflammation which is caused by red cells that are exudates from leather to the outer of blood
vessels and appear purpura and significant hemosiderosis. At present, there are no effects of treatment.
The Mongolian medicine theory is that Pigmentad purpuric dermatoses belongs to "Xuezheng" category,
mainly because of the disorder between the Stagnant blood and Huangshui function. The Mongolian
medicine divides Xuezheng into two types in clinic: blood heat and blood stasis. Given pure Mongolian
treatment, the total effective rate can amount to 88.5%.
A-184
Persist in the Combination of Scientific Culture and Humanities Culture in the
Modernization of Mongolian Medicine
Te Buqin1,2, Bai Changxi1*
1Pharmacy
Institute of Mongolia Medical, Inner Mongolia Medical University, 010110, Hohhot, China; 2Scientific and technical
department, Inner Mongolia Medical University, 010110, Hohhot, China; * Correspondence to: changbai2010@yahoo.com.cn
The scientific culture puts emphasis on seeking the truth and forms the cultural system based on the
scientific knowledge, methods and thoughts. Humanities culture mainly focuses on will, emotion,
consciousness and belief and forms the cultural system based on humanity knowledge, thoughts and
spirit. Traditional Mongolian medicine belongs to the traditional medicine of China, and is one of the
important components of the traditional culture of China. It is built and developed in the soil of the
traditional culture, and has a lot of humanistic and cultural factors. Therefore, under the new historical
conditions, we should insist on the combination of the scientific culture and humanities culture to achieve
modernization of Mongolian medicine.
A-185
The antibacterial and diuresis action of Mongolian medicine “yunga-4”
133
Guqid1
1Mongolia
health science and technology university doctor graduate student, International Mongolian medical hospital of Inner
Mongolia
Objective: To study the yunga-4 "Mongolian antibacterial function and diuresis action. Methods: In vitro
antimicrobial experiment using AGAR dilution Mongolian medicine "yunga-4" minimum bacteriostasis of
concentration (MIC) and the broth dilution minimum bactericidal concentration (MBC) were used. In body,
antibacterial experiment used bacterial systemic infections of animal models was used to observe the
yunga-4 "Mongolian medicine’s effect in mice and the metabolism of Mongolian cage observation of the
yunga-4 "diuretic effect. Results: The yunga-4 "Mongolian medicine of e. coli, fu's dysentery bacillus, the
common proteus, staphylococcus aureus, urinary tract infection clinical common bacteria have in vitro
antimicrobial effect, Mongolian" yunga-4 "could decrease the e. coli, staphylococcus aureus infection
mice mortality. Mongolian medicine "yunga-4, can increase the normal rats 3 h, 6 h, 9 h urine volume.
Conclusion: The yunga-4 Mongolian medicine has antibacterial and diuresis action.
A-186
The sand treatment, Mongolian medicated bath, Mongolian medicine for oral
administration: three combined treatments of rheumatoid arthritis
A la Teng Chu Lu1
1Chinese-Mongolian
hospital, Alashan Zuoqi, Alashan League, Inner Mongolia (750306)
Rheumatoid arthritis is a common clinical disease, especially in the countryside and pastoral areas, it has
high incidence of disease. In recent years, in different reasons, rheumatoid arthritis and skin disease are
increasing. It seriously affects the farmers’ and herdsmen’s health. I have been a doctor in countryside
and pastoral area for many years. During this time, I have explored and studied on how to use the sand
treatment and Mongolian medicated bath to treat different kinds of rheumatoid arthritis and skin disease.
From this experience, I have accumulated a number of clinical experiences and had some certain
achievements.
A-187
The experience of cervical cancer treatment combined with Mongolian medicine
European medicine
Bai. YH 1, Wang. CM 2
1 Mongolian
health sciences university ,Uulanbaatar, 210644, 2 Inner Mongolia zharuud, 029100
Objective: To the observation of the impact on neutrophil and cd4/cd8 which were influenced by the
cervical cancer treatment combined with Mongolian medicine and European medicine. Methods: 60
cases of cervical cancer patients were grouped presently into treatment group and compared
group(n=30cases) randomly, with each group treated 3 weeks of chemotherapy containing oxaliplatin.
The treatment group was treated by Mongolian medicine New ulijureel + oxaliplatin each time during
chemotherapy in conjunction for a total of 21d, compared to peripheral blood neutrophils in patients with
six cycles of chemotherapy and two groups of patients’ cd4/cd8 absolute value and proportion. The
compared group was treated with chemotherapy drug treatment alone. We observed and compared the
134
end of the treatments and the clinical efficacy of the two groups. Result: the neutrophils and cd4/cd8
proportions after the treatment group of using Mongolian medicine new ulijiureel+chemotherapy are
higher than chemotherapy alone group significantly (p<0.05). Conclusion：the treatment combined with
Mongolian medicine and European medicine can improve chemotherapy_related symptoms，reduce
the chemotherapy, bone marrow suppression, regulate the immune status of patients treated with
chemotherapy. Chemotherapy is carried out smoothly. It is worthy of further study.
A-188
A Clinical Study on Changes of TCM Syndrome and Relevant Factors in Patients with
Esophageal Carcinoma before and after Radiotherapy
Wu Langjie1, Du Yeqin1*
1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi
135
830011, Xinjiang Uighur Autonomous Region, China
Objective: Before and after radiotherapy, to investigate the relation of TCM syndrome differentiation,
cytokines and clinical features in patients with esophageal carcinoma. Methods: The classification of
TCM syndromes was carried out in 80 esophageal carcinoma patients before and after radiotherapy. The
80 patients' serum levels of TNF-α, IL-6 and IL-1 were measured by method in ELISA Test and
Electrochemiluminescence immunoassay. Results: (1)Before radiotherapy the syndromes of qi and
phlegm stagnation were 27 cases, 33.75%; phlegm and blood stasis were 34 cases, 42.5%; Yin
deficiency with noxious heat were 15 cases, 18.75%; qi and yang deficiency were 4cases, 5.00%. However, after radiotherapy the syndromes of qi and phlegm stagnation decreased to 5 cases, 6.25%; phlegm
and blood stasis decreased to 16 cases, 20.0%; the syndromes of Yin deficiency with noxious heat
increased to 45 cases 56.25%, qi and yang deficiency increased to 14 cases 17.5%. Before and after
radiotherapy TCM syndrome constitutes a significant difference compared (P<0.05). (2)The levels of
TNF-α were 34.34±25.28pg/ml, which were remarkably higher than the levels of TNF-α after radiotherapy
26.75±21.37pg/ml (P=0.012). The levels of IL-6 were 21.11±13.03 pg/ml, which were also remarkably
higher than the levels of IL-6 after radiotherapy 17.34±13.02pg/ml (P=0.031). After radiotherapy the 2
kinds of levels in the Yin deficiency with noxious heat patients were statistically different, but the others
were not. (3)The syndromes of TCM after radiotherapy were significant correlated with the levels of
TNF-α. Meanwhile, the syndromes of TCM after radiotherapy were also significant correlated with Clinical
features, such as esophageal cancer differentiation, KPS scores, bone marrow suppression indexing,
short term effect. Conclusion: Radiotherapy makes patients with esophageal carcinoma become Yin
deficiency with noxious heat. Yin deficiency with noxious heat type after radiotherapy in patients with
esophageal cancer from the excessive secretion of TNF-α, IL-6 significantly curbed. After radiotherapy,
changes in TCM syndromes were related with cytokines TNF-α and clinical features of esophageal
cancer and degree of differentiation, KPS scores, bone marrow suppression as well as short-term effect
on indexing.
A-189
Analysis of Bacteria Flora in Nasal Cavity on 102 Cases Acute Asthma
Ma Huan1, Li Fengsen1*
1Affilated
Traditional Chinese Medicine Hospital, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous
Region, China; *Correspondence should be addressed to fengsen602@163.com
Objective: This paper is to summarize the difference between acute asthma patients and average person
of bacteria flora in nasal cavityon acute stage so as to find the correlation between distribution of nasal
cavity bacteria flora and morbdity of asthma ,and to summarize the characteristic of nasal cavity bacteria
flora on patients of asthma with different TCM syndrome type to supply the experiment basis. Methods:
To separate and cultivate nasal secretions from 102 cases acute asthma patients and 42 cases average
person and then to test pure colonies with bacilli biochemical assessor. Results: 1.In both of the asthma
group and control group ,the detectable rate for nasal cavity bacteria flora was the highest of
staphylococcus, the main bacteria flora in asthma group was single bacteria flora while the multiple
bacteria flora in control group ,there were significant difference on single ,two and multiple bacteria flora in
both groups(P<0.01);2.The main bacteria flora in asthma patients with different TCM syndrome type was
staphylococcus , the detectable rate had no significant difference on asthma patients with different TCM
136
syndrome type(P>0.05)，
the detectable rate had no significant difference on nasal cavity bacteria flora of
asthma patients with different TCM syndrome type(P>0.05)；
3.The detectable rate of various bacteria flora
had no significant difference on age, disease course, sex and incidence of asthma patients(P>0.05).
Conclusion: 1.The patients of asthma manifested by imbalance of bacteria flora in nasal cavity ,and the
main bacteria flora is staphylococcus which may result in reducing barrier function and infection of
respiratory tract, consequently inducing asthma; or the recrudescence of asthma may destroy the
bacteria flora diversity in nasal cavity thereby cause vicious circle, while the reason still require further
research.2.the bacteria has no difference in patients of asthma with different TCM syndrome type
because of oneness in nasal cavity.
A-190
BiPAP combined with Acupuncture in the treatment of Chronic Obstructive Pulmonary
Disease complicated with Pulmonary Encephalopathy research
Wei Chenggong1, Fu Ziyi1, Liu Xiaohong1, Sun Zhijia1, Dai Yong1, Liu Jianbo1
Objective: To compare Noninvasive Bi Level Positive Airway Pressure Ventilation combined with
Acupuncture on Chronic Obstructive Pulmonary Disease complicated with Pulmonary Encephalopathy
patients blood gas analysis, computer synchronization rate, intubation rate and other indexes, the
evaluation of the treatment. Methods: 60 patients were randomly divided into treatment group and control
group in respiratory department of internal medicine hospital COPD in pulmonary encephalopathy from
2010.6 -- 2012.07. The control group received routine measures combined with BiPAP treatment, the
treatment group treated with Xingnao Kaiqiao acupuncture method. To observe the two cases in the
ventilation before the 1h,4h and 24h, ventilation and discharged in stable condition when the results of
arterial blood gas analysis and treatment course of man-machine synchronization rate, intubation rate
index. Results: Of the 26 cases in the treatment group, 25 cases were successful with 1 patient
required intubation and mechanical ventilation; in the control group of 27 cases 20 cases were
successfully treated with 7 patients required intubation and mechanical ventilation. The rate of the
treatment group was significantly higher than that of control group with machine synchronization, and the
tracheal intubation rate was lower than that of the control group, which was statistically different. The
successful treatment of treatment group and control group patients with blood gas analysis results show
that compared with the control group the treatment of initial 4h, pH, PaCO2 in treatment group was
improved significantly (P<0.05), PaO2 had no obvious difference; in the treatment of 24h and after the end
of the treatment group treated with pH, PaCO2, PaO2 were obviously improved (P<0.05) than those in the
control group, which was statistically different. Conclusion: BiPAP combined with Xingnao Kaiqiao
acupuncture can improve the COPD with pulmonary encephalopathy with man-machine synchronization
rate, reduce the intubation rate, carbon dioxide retention of patients was improved, and there were no
observed adverse reactions. The study is worthy of clinical application.
A-191
Clinical Evaluation and Lung Function of Plastering Therapy of TCM for Chronic
Obstructive Pulmonary Disease in Stable Phase in China: A Systematic Review of RCTs
Li Fengsen1*, Gao Zhen1,2, Jing Jing1, Xu Dan1
1
Affiliated Traditional Chinese Medicine Hospital，Xinjiang Medical University& National Clinical Research Base of Traditional
137
Chinese Medicine, Urumqi, 830000, Xinjiang Uighur Autonomous Region,China; 2Xinjiang medical university, Urumqi, 830011,
Xinjiang Uighur Autonomous Region, China；*Correspondence should be addressed to fengsen602@163.com
Objective: To assess the clinical evaluation and lung function of plastering therapy of TCM for chronic
obstructive pulmonary disease in stable phase. Methods: The Literature published before October of
2011 was searched in CNKI, VIP, and CBM to identify the randomized controlled trials (RCTs) about
plastering therapy of TCM for chronic obstructive pulmonary disease in stable phase in China. The
literature was screened according to the inclusive and exclusive criteria by two reviewers independently,
and the methodology quality was evaluated after abstracting the data, then the RevMan 5.0 software was
used for Meta-analyses. Results: Thirty RCTs involving 496 patients were included, the clinical trial
groups were 1690, and the control groups were 1611. The results of Meta-analyses showed that, for
clinical evaluation, compared with western medicine(WM), the plastering therapy(AT) of traditional
Chinese Medicine(TCM) plus WM was significantly better［
MD=4.00，
95%CI（
2.93，
5.46）
，
P＜
0.00001］
. AT
plus TCM were better than TCM ［
MD=2.05，
95%CI（
0.92，
4.99）
，
P=0.089］
,and AT plus WM plus TCM were
better than WM［
MD=3.19，
95%CI（
1.49，
6.82）
，
P=0.003］
, all the groups included in meta-analysis containing
AT were better than not［
MD=3.63，
95%CI（
2.84，
4.65）
，
P＜
0.00001］
. Conclusions: the existing evidences
show that plastering therapy of TCM can improve clinical efficacy of chronic obstructive pulmonary
disease. However, for the poor quality of included studies, the higher quality of RCTs should be
performed for better results.
A-192
Comparison between the Mongolian and Modern Medical Understandings of the
Regularity of Infectious Disease Course Development
Tu Men Wu Li Ji1, L. Guogua 2
1Mongolian Medicine Department, the First Affiliated Hospital of Inner Mongolia Medical University,
010050, China; 2Pneumology
Department, the First Affiliated Hospital of Inner Mongolia Medical University, 010050, China
As two different medical systems, Mongolian medicine and modern medicine have their own
understandings and treatments of infectious diseases. Meanwhile, because they are treating the same
type of diseases, there are many similarities between them. For instance, Mongolian medicine and
modern medicine almost agree on their understandings of the regularity of infectious disease course
development. However, Mongolian medicine persists in dialectical treatment in curing infectious
diseases, which obviously differs from modern medical emphasis on anti-pathogenic micro-organism
treatment.
A-193
Correlation between prothrombotic state markers and TCM syndrome type in Uygur and
Han patients with premature coronary heart disease
L.Hao 1, Wang Xiaofeng1, Zhai Xueqin1, C.Jihong 1, Z.Pengcheng 1, H.Yi 1, A.Naerhan 1
1
Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous
138
Region, China;* Correspondence should be addressed to wxj6283@sina.com
Objective: To investigate the correlation between prothrombotic state markers and TCM syndrome type
in Uygur and Han patients with premature coronary heart disease (PCAD). Methods: 626 patients were
diagnosed as CHD in Xinjiang Medical University, including PCAD 340 cases (Uygur 154 cases, Han 186
cases) and late-onset CHD 286 cases (Uygur 106 cases, Han 180 cases). Patients with traditional risk
factors were recorded after hospital admission, and were conducted syndrome type differentiation of
traditional Chinese medicine by attending physicians or physicians in Cardiovascular Department. The
morning fasting venous blood 2ml with separation of serum were collected from patients enrolled in the
second day after admission. The methods used were as follows: Fibrinogen (Fg/FIB) using automated
latex enhanced turbidimetric immunoassay method, tissue plasminogen activator(t-PA), plasminogen
activator inhibitor(PAI-1) using ELISA method for the determination of-two, D dimer(DD),
P-selection(P-Sel) using the ELISA double antibody sandwich assay test to check. Results: For PCAD
patients of Uygur and Han Nationality, the levels of PAI-1, Fg and DD had statistically significant
difference compared with those in the late onset group of the same nationality. The PAI-1 level of turbid
sputum group, and the levels of PAI-1, P-Sel and F g, of Qi and yin deficiency group had significant
difference compared with the late onset groups of the same nationality; turbid sputum syndrome of Uygur
patients with PCAD is positively related with P-Sel, t-PA, PAI-1, F g and negatively correlated with DD;
turbid sputum syndrome of Han patients with PCAD is positively related to P-Sel, PAI-1and F g, and
negatively correlated with t-PA; Qi deficiency and blood stasis was positively correlated with DD.
Conclusion: For Uygur and Han nationality patients with PCAD, there is correlation between syndrome
type of Traditional Chinese Medicine and the markers of prethrombotic state, which can serve as a guide
in determining the Traditional Chinese Medicine syndrome type of PCAD.
A-194
Different Syndromes in Patients with Asthma-related Changes in Immune Indexes
Su Jun1, Li Fengsen1*, Ma Huan1
1Affiliated
TCM Hospital ，
Xinjiang Medical University, 830000, Urumqi, Xinjiang Uighur Autonomous Region ，
China；
*Correspondence should be addressed to fengsen602@163.com
Objective: Through the blood of patients with different syndromes of acute asthma attack of CD4+、
CD8+
T-lymphocytes, eosinophil cationic protein (ECP), total serum immunoglobulin E, (T-IgE) and its saliva,
sputum, Determination of secretory immunoglobulin A (SIgA) in nasal secretions to understand and
summarize the different syndromes in patients with asthma-related changes in immune parameters
characteristics for Traditional Chinese Medicine for asthma in acute exacerbation of the syndrome
differentiation to provide an objective basis. Methods: The collection of different syndromes in patients
with asthma, 200 cases were detected by ELISA in the ECP in peripheral blood of patients with asthma,
T-IgE as well as sputum, saliva, nasal secretions, SIgA; the use of flow cytometry to its blood of CD4+ and
CD8+ T-lymphocytes detected; Results: (1) asthma patients in the blood of CD4+ and CD8+
T-lymphocytes, CD4+/ CD8+ expression with the normal group were significantly increased
comparatively, and saliva, sputum, nasal secretions, SIgA content compared with the normal group was
significantly lower, the difference was statistically significant (P <0.05); (2) different syndrome types in
patients with asthma peripheral blood of CD4+, of CD8+ T-lymphocytes of CD4+/ CD8+, T-IgE, the ECP
139
and saliva SIgA content relative to comparison no obvious difference (P0.05). (3) sputum, nasal
secretions of SIgA levels compared between the different syndromes in patients with asthma, the
difference was statistically significant (P <0.05), cold whooping sputum of patients with SIgA content
higher than the wind sputum roaring, virtual heavy breathing, cold pack, hot heavy breathing patients (P
<0.05), while the SIgA content in nasal secretions were lower than the wind sputum roar, cold pack hot
Asthma patients (P<0.05); compared to the other groups there are no significant difference (P>0.05).
Conclusions: (1) Different syndromes in patients with asthma in acute exacerbation of immune
dysfunction exists a difference (2) Changes in different syndromes in patients with asthma in acute
exacerbation of immune parameters, SIgA changes in respiratory secretions on which the difference is
particularly evident. Therefore, the detection of patients with asthma-related immune parameters helps to
provide objective and theoretical basis for the syndrome differentiation of Traditional Chinese Medicine for
asthma.
A-195
Explore the scientific meaning of “Lung Governing Skin and Hair” from SIgA in
secretions of asthma, urticaria patients
Zhang Yanli1, Li Fengsen1*
1
Affiliated Traditional Chinese Medicine Hospital，Xinjiang Medical University& National Clinical Research Base of Traditional
Chinese Medicine, Ulumuqi, 830000, Xinjiang Uighur Autonomous Region, China *Correspondence should be addressed to
fengsen602@163.com
Objective: The common pathological embodiment and rules of mucosal immunity changes in the disease
process of bronchial asthma and chronic urticaria were sought, and the relevancy between pulmonary
diseases and dermatosis was discussed through determination of secretory immunoglobulin-A (SIgA) in
saliva, sputum and nasal secretions, thereby further discussing the scientific connotation of ‘Lung
Governing Skin and Hair ’Theory. Methods: 32 patients with pure bronchial asthma (asthma group), 33
patients with bronchial asthma combined with chronic urticaria (combined group), 31 patients with chronic
urticaria (urticaria group) and 30 normal people (normal group) were collected, ELISA method was
adopted to test SIgA in their sputum, saliva and nasal secretions. Results: 1）
The SIgA contents in saliva
and nasal secretions were higher in Asthma, Urticaria, and Combined groups, compared to Control
group（
P<0.01）
. SIgA in nasal secretion had no significant difference among the three disease
groups（
P>0.05）
. SIgA in saliva secretion was higher in Uriticaria, compared to Combined
Group（
P<0.01）
2) SIgA in sputum secretion was lower in Asthma and Combined groups, compared to
control and Urticaria groups（
P<0.01）
3)SIgA concentration in the saliva and sputum of subjects in the
Control Group had a negative correlation（
r=-0.369, P =0.045）
Conclusion: SIgA have common
embodiment in patients with bronchial asthma and chronic urticaria, namely common expression in
pulmonary diseases and dermatosis, which further embodies the ‘lung governing skin and hair’ theory,
and mainly reflects in pathology，
and the mucosa immune-related media may be the material basis of
contact between the two.
A-196
Intestinal Microflora structure and function analysis in Uighur Traditional Medicine
syndrome of asthma
140
Li Chunyan1, Halmurat Upur2*
1Basic
Medical Institute，
Immunology Department of Xinjiang Medical University, Urumqi 830011, China; 2Traditional Uighur
Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China; *Correspondence
should be addressed to Halmurat@263.net
Objective: Syndrome differentiation is the most important foundation of Traditional Uighur Medicine.
Within the abnormal Hilit formation, the abnormal Savda is the common pathological basis and clinical
syndromes in the most complex diseases. It is profound to further make clear and comprehend the
biologic basis for abnormal Savda syndrome of Traditional Uighur Medicine theory. Methods: In this
study, fecal bacterial diversity in abnormal Savda asthma patients (n=64) and non-abnormal Savda
asthma patients (n=80) were profiled by 454 pyrosequencing of the V3 region of the 16S ribosomal RNA
gene. Results: Principal component analysis showed structural segregation between the two groups.
Shannon diversity analysis showed both the microbial groups and abundance were decreased in the gut
microbiota of abnormal Savda asthma patients (One-way ANOVA test, P＜
0.05). Twenty-three operational
taxonomic units (OTUs) were indentified by redundancy analysis as key variables significantly associated
with the structural differences. 5 OTUs belonging to the genera Bacteroides, two OTUs belonging to the
genera Parabacteroides of the phylum Bacteroidetes, and one OTU belonging to the genera Veillonella of
the phylum Firmicutes were enriched in the gut microbiota of abnormal Savda asthma patients, whereas 4
OTUs belonging to the genera Subdoligranulum, 4 OTUs belonging to the genera Faecalibacterium, and
one OTUs belonging to the genera Roseburia of the phylum Firmicutes were enriched in that of
non-abnormal Savda asthma patients. At the family level, one OTU belonging to the family
Lachnospiraceae were more abundant in the gut microbiota of abnormal Savda asthma patients, and 4
OTUs belonging to the family Ruminococcaceae and Prevotellaceae were less abundant. Conclusion:
Asthma patients with abnormal Savda syndrome had similar changes and characteristic in gut microbiota
structure, which showed obvious significance differences between two groups. The results indicated the
changes of key bacterial groups maybe the important factors associated with the pathological process of
abnormal Savda syndrome, and provided some biologic basis for the abnormal change of Hilit of
Traditional Uighur Medicine theory.
A-197
Study on Metabonomics Common Characteristics of COPD about Traditional Chinese
Medicine Kidney Depletion Phlegm and Blood Stasis Syndrome and Uighur Medicine
Abnormal Savda Syndrome
Xu Weifang1, Halmurat Upur2*, Li Fengsen1
1Affilated
Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，
Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China；2 2Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011,
Xinjiang Uighur Autonomous Region, China, *Correspondence should be addressed to Halmurat@263.net
Objectives: This study from the metabonomics Angle, we regarded COPD, and explored COPD of
metabonomics change by nuclear magnetic resonance (NMR)technology, and utillizing TCM and Uighur
theory as the guide, analysed COPD in different points among the TCM types and Uighur types and the
general population and the structure of the differences between the phenotype metabolism, through
141
looking for and identifying the metabolic biological markers, we would elaborate the metabonomics
aspects of the common characteristic changes and material foundation of TCM kidney empty phlegm and
blood stasis syndrome and Uighur Medical Abnormal Savda syndrome, make COPD of medical
syndrome differentiation and disease more objective and standard so as to guide clinical work for clinical
diagnosis of COPD, and provide the new ideas and methods. Methods: 1) There were total of 152 cases
of patients with COPD, cases source from October 2008 to March 2010 in affiliated hospital of Xinjiang
Medical University, then those had been diagnosed according to western medicine diagnosis
classification, and had been divided into four groups according to TCM and Uighur Medicine on
syndrome differentiation and classification. Meanwhile, a total of 52 healthy volunteers composed the
Normal control group, and they were from the medical center of the Affiliated Hospital of Chinese
Traditional Medicine in Xinjiang Medical University. 2) All patients were classified into 4 groups according
to the TCM and Uighur Medicine on syndrome differentiation and classification; 3) All patients and
normal control group subjects, blood samples were tested through utiulizing the NMR metabonomics;
Participants by phosphate buffer plasma treatment for 1 h NMR testing, the acquisition spectra data
pretreatment, using principal component analysis (PCA), orthogonal partial least squares discriminant
analysis (OPLS-DA) processing map data, data interpretation; then by Pearson correlation coefficient
significant difference and two dimentions technique, metabolism composition difference were detected to
the healthy people and patients of COPD with the kidney empty phlegm and blood stasis syndrome and
Abnormal Savda syndrome, than the 5 groups plasma metabolites map of the differences had been
researched. 4) Data processing were used mainly with Topspin 2.0 software, SIMCA-P+11 software,
SPSS 13. 0 for Window. Results: 1) 1 H NMR spectrum and 2D pattern recognition in analysis showed
that COPD patients with kidney empty phlegm and blood stasis syndrome group, the non-kidney empty
phlegm and blood stasis syndrome group, Abnormal Savda syndrome and the non-Abnormal Savda
group in comparison with the normal control group respectively, their distribution area separated
completely, and COPD kidney empty phlegm and blood stasis syndrome group compared with the
non-kidney phlegm and blood stasis syndrome group, and COPD with Abnormal Savda syndrome
compared with the non-Abnormal Savda group，
their distribution area separated also completely, many
kinds of amino acids in plasma decreased significantly, including leucine, alanine, melons, acid tyrosine,
l-histidine, glycine, methionine, isoleucine valine, etc, and healthy people comparing statistical
significance (P＜
0.05). In addition, glycoprotein, glutamine, inositol, lactic acid, choline, creatine also
decreased significantly, the healthy and comparing statistical significance (P＜
0.05). At the same time,
compared with the normal control group, 1 H NMR spectrum and 2 D pattern recognition in analysis
shows that, COPD patients, COPD kidney empty phlegm and blood stasis syndrome group and the nonkidney phlegm and blood stasis syndrome group, COPD with abnormal black Savda and the group with
non-abnormal black Savda group completely separated in different area, metabolic component of plasma
of two group have obvious difference; Many kinds of amino acids in plasma of Kidney empty phlegm and
blood stasis syndrome and non-kidney empty phlegm and blood stasis syndrome group and the
anomalous black Savda and the nonabnormal black Savda patients with COPD significantly reduced,
metabolic components however, were basic same, many kinds of amino acids significantly reduced, the
difference was statistically significant (P＜
0.05); Including isoleucine, leucine, valine, alanine, glutamine,
tyrosine, phen- ylalanine, the difference was statistically significant (P＜
0.05). In addition, lactic acid,
glycoprotein, unsaturated fat, carnitine and extremely low density lipoprotein also significantly lower than
healthy people, the difference was statistically significant (P＜
0.05). Meanwhile the distribution of COPD
kidney empty phlegm and blood stasis syndrome group and Abnormal Savda area not separates
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completely, the metabolic component mentioned above of the kidney empty phlegm and blood stasis
syndrome group and abnormal black Savda patients were not statistically different (P>0.05) Metabolism
including isoleucine, leucine, valine, alanine, tyrosine, glutamine, pheny- lalanine. and lactic acid,
glycoprotein VLDL and unsaturated fat also significantly reduced, carnitine was relatively higher, the
difference was not statistically significant (P>0.05). extremely low density lipoprotein plasma VLDL,
unsaturated lipid and acetone of COPD kidney empty phlegm and blood stasis syndrome patients and
abnormal black Savda patients have shown lower, but the difference was not statistically significant
(P>0.05). table 4, 12 of the difference of metabolites are expected to be serum specific biomarker of
COPD kidney empty phlegm and blood stasis syndrome patients and Abnormal Savda patients.
Conclusion: 1) Characteristics of COPD were close to kidney deficiency with phlegm and blood stasis
syndrome of Chinese traditional medicine and Abnormal Savda syndrome of Uighur medicine, both of
them were the common and typical type within the process of the development of the complexity disease
with similar clinical biological characterization of serious long illness and older patients, interaction as
well. 2) Metabonomics change and metabolic phenotype of COPD with kidney empty phlegm and blood
stasis syndrome and Abnormal Savda are more outstanding but similar, and also with common
biomarkers, mainly are threonine, leucine, valine, alanine, tyrosine, lactic acid, glycoprotein; VLDL,
unsaturated lipid metabolites, etc; The result of the amino acids, fat and sugar metabolic abnormalities
demostrated material metabolic disorder exist in the patients with complex diseases kidney empty phlegm
and blood stasis syndrome and Abnormal Savda, and also demostrated low body's immune function,
endocrine disorders and energy metabolism disorder; these methods are expected to provide new ideas
and methods for early warning and forecast and clinical diagnosis d finally for big medical system
complexity of the disease early warning, forecasting, treatments with new ideas and methods. 3)
Metabonomics common features of Complex diseases with TCM and Uighur syndrome further reflected
the interoperability three kinds of medical model of Chinese Medicine, Uighur Medicine and Modern
Medicine about COPD; Make COPD of syndrome differentiation would be more objective, standard, and
would be able to guide clinical diagnosis and treatment, the complexity of the syndrome differentiation and
disease in guiding and outcome objective aspects to have the important meaning.
A-198
Observing the clinical efficacy of combined treatment of Traditional Chinese Medicine
and Western Medicine to ventilator-associated pneumonia
Zhang Yanpeng1, Lv Shuqin1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China; *Correspondence should be addressed to lvshuqin@126.com
Objective: To observe the clinical efficacy of Erchen Tang (Decoction of Pinellia and Perocarpirum)
combined Maxingshigan Decoction to ventilator-associated pneumonia (VAP). Methods: 64 patients
who accord with the diagnosis of VAP were randomly divided into two groups (treatment group involving
33 patient & control group involving 31 patients) .The treatment group were given conventional therapy of
Western Medicine and Erchen Tang (Decoction of Pinellia and Perocarpirum) combined Maxingshigan
Decoction which were taken orally 30 minutes after meals two times a day; the control group were given
conventional therapy of Western Medicine. The body temperature, phlegm, bellows sign, X-ray display
and white blood cell count were accumulated before the experiment and 2nd, 4th and 6th after the
143
experiment. Result: The response rate of treatment group was 78.80%, and the rate of control group was
54.8%., which did make a statistical significance (P≤0.05). The treatment group made a statistical
significance on improving body temperature, phlegm and white blood cell count compared with control
group (P≤0.05); while it did not have the statistical significance on bellows sign and X-ray display
compared with control group (P>0.05). Conclusions：
There is a good clinical treatment effect of Erchen
Tang (Decoction of Pinellia and Perocarpirum) combined Maxingshigan Decoction to
ventilator-associated pneumonia (VAP), which can improve clinical symptoms, control infection
effectively, and make patient's condition stable. In a word, it makes a positive effect to VAP.
A-199
Plasma metabonomics study on the asthma diagnosed as Shenxu-tanyu syndrome in
Traditional Chinese Medicine with NMR
Yan Xinghai1, Halmurat Upur2*
1
Gan Su Wu Yi Central Hospital; 2Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China; *Correspondence should be addressed to Halmurat@263.net
Objective: To apply the metabonomics technology about nuclear magnetic resonance (NMR), Retrieval
substance metabolism way, metabolism state, discover biological marker in asthma diagnosed as
Shenxu-tanyu syndrome patients by analysing and judging the interrelated specially component about
plasma with asthma diagnosed as Shenxu-tanyu syndrome patients. Methods: Sodium phosphate buffer
was employed to prepare plasma samples which obtained from 39 cases of Shenxu-tanyu syndrome
patients with asthma and 40 healthy volunteers. A 1H NMR based metabonomic analysis was performed
on these plasma samples. A multi-steps date preprocessing procedure was completed and then
Orthogonal Partial Least-Squares Discriminant Analysis (OPLS-DA) was fulfilled to distinguish the
metabolic phenotypes, the influential biomarkers accounting for the metabolic signature perturbation were
discovered by Pearson’s product moment correlation coefficient and 2D NMR. Results: It was found that
the metabolic phenotypes in Shenxu-tanyu syndrome with asthma group underwent significant variation ,
which resulted in the obvious classification between the health and patients groups in the score scattering
plot of OPLS-DA model; the results show that in plasma of Shenxu-tanyu syndrome patients with asthma,
the expression of isoleucine, leucine, valine, lactate, alanine, glycoprotein, citrate, creatine , creatinine,
1-methy histidine, scyllo-inositol see a significant decrease compared with healthy group (P<0.05),
whereas , the expression of acetone, acetoacetate, carnitine significant elevated (P<0.05). Conclusion:
Compared with asthma diagnosed as Shenxu-tanyu syndrome patients, there were significant differences
in metablolite and metabolism net in healthy, possible by interaction between geneticfacter and
environmental facter. The marker metablolite of asthma diagnosed as Shenxu-tanyu syndrome have
osculate correlation with pathologic physiology change, such as airway inflammation ,airway
hypersensitivity, airway remodeling. Meanwhile, during asthmatic attack period, the process of energy
metabolism have significant changed in the asthma diagnosed as Shenxu-tanyu syndrome patients,
particular expression in increasing metabolism fatty acid and branched-chain amino acid, restraining
tricarboxylic acid cycle, strengthening glyconeogenesis, reducing compound and metabolism creatine,
which predicted that asthma diagnosed as Shenxu-tanyu syndrome patient have continued analosis and
immunologic function disorder in long time. The continuance and protraction stimulation in airway
inflammation are the main reasons to lead to this condition. The study provided the preliminary foundation
144
in recognizing further microcosmic physicala foundation of asthma diagnosed as Shenxu-tanyu syndrome
and evaluating the intervene effect of Traditional Chinese Medicine to asthma diagnosed as
Shenxu-tanyu syndrome.
A-200
Serum Metabonomics Research on Shenxu-tanyu Syndrome Type 2 Diabetes Mellitus
Based on NMR
You Li1, Halmurat Upur2*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China; 2Traditional Uighur Medicine Institute, Xinjiang Medical University, Xinyi Road393, 830011, Urumqi , Xinjiang Uighur
Autonomous Region, China；* Correspondence should be addressed to halmurat@263.net
Objective: To explore the metabolic variation of type 2 diabetes mellitus of shenxu-tanyu syndrome in
Traditional Chinese Medicine by using nuclear magnetic resonance(NMR), analysing the pathogenesis of
discrepant metabolites, finding characteristic biomarkers. Methods: Serum samples from 33 cases of
shenxu-tanyu syndrome type 2 diabetes mellitus patients and 50 cases of healthy persons were detected
with 1H-NMR and 2D H-NMR. The data were compiled, integrated and normalized, then identify the
discrepant metabolites by Orthogonal Partial Least-Squares Discriminant Analysis (OPLS-DA). Results:
It shows that it is separated obviously between the healthy people and patients groups in the score
scattering plot of OPLS-DA model, compared to healthy people, there are various kinds of amino acids
that are lower markedly, include isoleucine, leucine, valine, alanine, glutamate, histidine, tyrosine,
phenylalanine, 1-methylhistidine, difference was statistically significant(P<0.05). In addition, lactate,
citrate, creatine and creatinine are also lower markedly(P<0.05). But the α-glucose, β-glucose and very
low density lipoprotein are increased compared to healthy people(P<0.05). Conclusion: (1) There are 16
kinds of discrepant metabolites which may be the potential biomarkers of type 2 diabetics of shenxu-tanyu
syndrome. (2) The serum metabonomics change mechanisms of type 2 diabetics of shenxu-tanyu
syndrome are closely related with glucose, lipid and amino acid metabolism disorder. (3) Type 2 diabetics
of shenxu-tanyu syndrome’s tricarboxylic acid cycle and glycolysis is inhibited, protein is metabolic
disorder, branched chain amino acid is metabolic boost. (4) The study on the same disease with different
syndrome of type 2 diabetics is in progress.
A-201
Studies on Traditional Chinese Medicines
Brantner Adelheid 1*, Lower-Nedza Agnieszka 1, Shi Yujing 2
1Institute
Austria;
of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Universitaetsplatz 4/I, A-8010 Graz,
2China Academy
of Chinese Medical Sciences, Institute of Chinese Materia Medica, No. 16 Nanxiaojie, Dongzhimen Nei
Ave, Beijing 100700, China; *Corresponding author: Prof Dr Adelheid Brantner Institute of Pharmaceutical Sciences, Department
of Pharmacognosy University of Graz Universitaetsplatz 4/I A-8010 Graz, Austria adelheid.brantner@uni-graz.at; Phone: 0043
316 380 5528; Fax: 0043 316 380 9860
Traditional Chinese medicines (TCMs) are complex mixtures containing up to hundreds different
constituents but only a few compounds are responsible for their pharmacological activities. These large
145
numbers of constituents in the TCMs make the screening and analysis of the bioactive components
extremely difficult. Chromatography is one of the main techniques applied because of its powerful
separation and sensitive detection. WHO introduced the use of chromatographic methods for the
standardization of medicinal plants and herbal medicinal products. Nowadays the high performance liquid
chromatography (HPLC) together with thin layer chromatography (TLC) are the most common analytical
methods to determine either the quality or the quantity of compounds in the phytochemical evaluation.
The aim of this study was to optimize HPLC and TLC methods for the simultaneous quantification of the
main compounds present in the Traditional Chinese formulas Huanglianjiedutang (HLJDT) and Si Miao
with two modifications. Representative chromatographic fingerprinting of batches of the herbal
components of the formulations originated from different Chinese provinces were applied to offer integral
characterization of the single herbs. Validation of the HPLC methods was performed to demonstrate its
reproducibility, precision, stability, repeatability, and recovery rate. Permeability is a major factor for orally
applied drugs. Therefore permeability assay and parallel artificial membrane permeability analysis
(PAMPA) were used to determine passive, transcellular drug permeability. The permeability of well known
TCM formulations (decoctions of Sì Miào Sǎn and its modifications, Yin Qiao San/decoction, Yin Qiao
San/ethanol extract) was evaluated in the experiment. Hexadecane/hexane and lecithin/dodecane were
used to create artificial membranes. Different parameters of the methods were evaluated.
A-202
Study of acupoint application therapy on rat prothrombotic state and establishment of
the corresponding animal model
Zhang Wenhui1, Song Xiaoping1
1Affilated
Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China
Objective: To study the influence of acupoint application therapy on rat prothrombotic state. Methods:
Forty-eight SD rats were randomly divided into a model group, an acupoint application group I, group II,
and an acupoint application group III. Continuous intervention of two weeks for three groups except the
model one, the pre-thrombosis model was induced by Adrenalin Hydrochloride and ice water method for
four groups separately. Molecular markers in arterial blood were determined 18 hours later. Results:
Fibrinogen (Fbg), Von Willebrand factor (vWF), and type-1 plasminogen activator inhibitor (PAI-1)
decreased after point application. The levels of Fbg, vWF, and PAI-1 in the acupoint application group I
and group II were lower than those in the model group (P<0.01). The levels in the acupoint application
group III was lower than those in the model group (P<0.05). And there was no significant difference in the
acupoint application group I, group II, and III (P>0.05). Conclusion: The acupoint application can
significantly affect contents of Fbg, vWF, and PAI-1.
A-203
Study on Cytokine Regulation Mechanism of Downregulating Eosinophils By Tonifying
Shen in Traditional Chinese Medicine to Prevent Asthma Relapse
Zhang Wenbin 1, Mao Bing 1*
1Huaxi
hospital of Sichuan university of combination of TCM and western medicine department, 610041, Chengdu, China
146
Objective: In the way of methods of treatment replaced by prescription, using YGW, a typical Tonigying
kidney prescription, as intervention, to observe its effects on levels of interleukin-5 (IL-5), eotaxin in
peripheral blood (PB) of bronchial asthma mouse model, bronchoalveolar lavage fluid (BALF) and bone
marrow (BM), as well as counts of white blood cell in PB, eosinophil (EOS), nucleated cells and EOS in
BM; investigate the impact of kidney-tonifying therapy on EOS, revealing its mechanism of preventing
asthma recurrence, in order to provide a theory evidence for the implication of asthma prevention by
Tonifying Shen in Traditional Chinese Medicine. Method: Twenty-four SPF BALB/c mice were divided
into four groups (6 in each group), which were physiological saline group (as control, A), asthma model
group (B), prednisone treated group (C) and YGW treated group (D), based on the table of random
number. Mice in group A underwent intraperitoneal injection of physiological saline and nebulization. For
the other three groups, the mice received intraperitoneal injection of Ovalbumin (OVA) 20ug mixed with
abuminum hydroxide 50ug twice to sensitize and 1%OVA by nebulised medication to stimulate. All the
mice got stimulation for 1 week. After secondary sensitization, drugs (YGW capsule/prednisone) or
placebo (physiological saline) were administrated for 2 weeks. After the last time stimulation, all the mice
were killed within 24h. Using ELISA, the levels of interleukin-5 (IL-5), eotaxin in peripheral blood (PB) of
bronchial asthma mouse model, bronchoalveolar lavage fluid (BALF) and bone marrow (BM), were
measured, respectively. Meanwhile, white blood cell in PB, nucleated cells and EOS in BM were
counted. Lung tissues of mice were evaluated pathologically by HE staining. Result: 1. EOS count: The
count in PB was significantly increased (p<0.05) in asthma group and YGW group compared to control;
asthma group got significantly increased EOS count than prednisone and YGW group did (p<0.05);
however there was no significant difference between prednisone group and YGW group (p>0.05). For the
EOS count in BM, it was the same as that in PB.2. White blood cell count and nucleated cell count: For the
white blood cell count in PB, there was no significant influence among each group (p>0.05), the same for
nucleated cell count in BM (p>0.05).3. IL-5 level: The level in BALF was significantly increased in each
experimental group compared to control (p<0.05); asthma group got significantly increased IL-5 level than
prednisone and YGW group did (p<0.05); however there was no significant difference between
prednisone group and YGW group (p>0.05). In PB, the IL-5 level got varying increase in prednisone group
and YGW group compared to control (p<0.05); but varying decrease when compared to asthma group
(p<0.05); there was no significant difference between prednisone group and YGW group. The level in BM
was no significant difference among control, prednisone group and YGW group (P>0.05); however, the
asthma group got highest IL-5 level and it was significantly different when compared to control and
prednisone group (p<0.05). 4. Eotaxin level: In BALF, there was no significant difference among control,
prednisone group and YGW group (p>0.05); Eotaxin level was highest in asthma group and it was
significantly different compared to control (p<0.05); compared to asthma group, prednisone group and
YGW group got varying decrease (p<0.05). In PB, there was significantly increased in asthma group and
YGW group compared to control (p<0.05); asthma group got significant increase than prednisone
(p<0.05); there was no significant difference prednisone group compared to YGW group and control
(p>0.05). In BM, there was no significant difference among control, prednisone group and YGW group
(p>0.05); asthma group got highest level and it was significant different compared to control and
prednisone group (p<0.05).5. Pathological section: For the mice in asthma group, they got hyperemia and
edema in alveolar septum, widening septum, vessel hyeremia in pulmonary interstitium, infiltration of
EOS, monocytes and lymphocytes; however, the mice in YGW group and prednisone group got
narrowing alveolar septum, less vessel hyeremia in interstitium, and less infiltration of EOS, compared to
147
asthma group. Conclusion: 1. YGW could down-regulate the level of IL-5 and Eotaxin in BALF, PB and
BM of mice in asthma model.2. YGW reduced EOS level in PB and BM of mice in asthma model.3. YGW
had alleviation on the pathological changes of lung tissues of mice in asthma mode.4. The mechanism of
asthma prevention by Tonifying Shen in Traditional Chinese Medicine could be through regulation of IL-5
and Eotaxin and down-regulation of EOS level in PB and BM.
A-204
Study on Esophageal Carcinoma Uygur Traditional Medicine Syndromes and the Change
of T-lymphocyte Subsets and NK cells of Patients Before and After Radiotherapy
Zhou Jie 1, Du Yeqin 1*
1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi
830011, Xinjiang Uighur Autonomous Region, China
Objective: To investigate the esophageal carcinoma Uygur Traditional Medicine syndromes and the
change of T cell subsets and NK cell level before and after radiation therapy, and the observation of
radiotherapy after those medicine typing in patients with esophageal cancer with radioactive esophagitis,
bone marrow suppression and near future curative effect. Method: To apply Uygur Medicine abnormal
humoral classification standard before and after radiation treatment in 120 cases of esophageal
carcinoma patients with Uygur Medicine typing, and before and after radiation therapy using flow
cytometry in patients with peripheral blood T cell subsets and NK cell level, simultaneously recorded
during radiotherapy related clinical features of toxic reactions and curative effect in the near future.
Results: (1) before and after radiotherapy, the distribution of esophageal cancer patients can be drawn:
abnormal savda(50%)>abnormal belghem(27.5%)>abnormal kan1(15%)>abnormal sapra(7.5%);after
radiotherapy: abnormal savda(76.67%)>abnormal sapra(12.5%)>abnormal kan(7.5%)>abnormal
belghem(3.33%)；
The proportion of Uygur Traditional Medicine Syndromes has significant
difference(P<0.01); (2) before and after radiotherapy in 120 cases of patients with esophageal carcinoma
T cell subsets and NK cell level changes : CD3+、
CD4+、
CD4+\CD8+and NK cell levels were decreased,
CD8+ before radiotherapy levels are elevated before radiotherapy, and radiation changes before and
after treatment was statistically significant(P<0.05); (3) after radiotherapy, different Uygur Traditional
Medicine syndromes, abnormal savda and abnormal Savda before and after radiotherapy of T cell
subsets and NK cell level changes: abnormal savda patients after radiotherapy
CD3+、
CD4+、
CD4+\CD8+and NK cell levels and before radiotherapy ratio decreased, while CD8+ level
than before radiotherapy L High, and before and after radiotherapy changes were statistically
significant(P<0.05); instead of abnormal Savda in patients before radiotherapy compared with
CD3+、
CD4+、
CD4+\CD8+ 、
CD8+ and the ratio of NK cell levels and before radiotherapy changes little, and
the changes were not statistically significant, (P<0.05); (4) after radiation for abnormal savda of abnormal
Savda in patients with esophageal cancer radiotherapy in toxicity and efficacy were compared, found
between the two differences, and the differences were statistically significant (P<0.05). Conclusion:
Abnormal Savda is the major Uygur Traditional Medicine Syndromes before and after undergoing
radiotherapy; radiotherapy makes abnormal savda humoral form; after radiotherapy abnormal Savda
patients with impaired immune function is more and more serious, and the radioactive esophagitis, bone
marrow suppression and the recent effect correlation.
A-205
148
Study on immune and endocrine disturbance of bronchial asthma by Traditional Chinese
Medicine, Uygur Medicine and Western Medicine
Li Fengsen 1, Halmurat Upur2*
1Department
of Respiratory Department ， Affilated Traditional Chinese Medicine Hospital of Xinjiang Medical
2Traditional
University；
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous
Region, China; *Correspondence should be addressed to halmurat@263.net
Objective: In this study, we studied the relationship between asthma and immunity, endocrinium, and
changing regulation in traditional Chinese medicine, Uygur Medicine and Western medicine. Discovered
the rule of immune and endocrine system disturbance in patients with asthma (especially the severe
asthma), explained pathogenesis of asthma, summarized the regulation between the changes of immune
and endocrine systems in deficiency asthma, abnormal sewda asthma; searched the biological
expression in the classification by Traditional Chinese Medicine and Uygur Medicine and relative indices
of microcosmic syndrome differentiation, discriminate the concordance and reciprocation relationship
among severe asthma, deficiency asthma and abnormal sewda asthma, and provide rationale basis for
the diagnosis, prediction and treatment of asthma. Method: During the period of January 2006 to January
2007, 156 cases of bronchial asthma patients were collected. All cases were analyzed by severity
classification, differentiation of symptoms and signs for classification of syndrome by traditional Chinese
medicine and Uighur medicine. PEF% Pred, FEV1% Pred and FEV1% were detected in asthma patients
by Pulmonary function testing machine (JAEGER PET) made in Germany. IL-1, IL-6, TNF-α, ACTH, CS
and GH were detected with radio immunoassay while CRH with ELISA. Lymphocyte apoptosis,
CD3CD4CD8, CD11b, CD18 and CD62P were determined with flow cytometry. Measurement data were
described with mean and standard deviation, and multiple mean comparisons were dealt with single
factor analysis of variance or group t test. Discriminant function was calculated with Fisher method. The
size of test was α=0.05. Results: The course was long in patients with severe asthma, and compared
with the patients with asthma of other types, the mean age of severe asthma was older. Compared with
other types of asthma classified by Traditional Chinese Medicine classification, the mean age of patients
with deficiency asthma was older. Compared with the other types of classified by Uygur Medicine
classification, the mean age of patients with abnormal sewda asthma was obviously older. There was
significant statistical difference（
P＜
0.05）
. Lymphocyte subgroup and its mediatior of CD4, CD8, CD4/CD8,
CD11b, CD11b /CD18, CD62P and TNF-αheightened gradually in accordance with the exacerbation of
the disease. Those were the highest in severe asthma, while lymphocyte apoptosis decreased along with
the aggravation of the disease. The apoptosis was not obvious in the severe asthma. CD4/CD8, CD11b,
CD11b/CD18 and CD62P were the highest and lymphocyte apoptosis the slowest in the group of
deficiency asthma. CD11b increased and lymphocyte apoptosis decreased obviously in the abnormal
sewda asthma group. CS, ACTH and CRH decreased gradually in accordance with the exacerbation of
the disease, and were the lowest in the severe asthma. CS, ACTH and CRH were the lowest in the
deficiency asthma with differentiation of symptoms and signs for classification of syndrome by Traditional
Chinese Medicine and in the abnormal sewda asthma with differentiation of symptoms and signs for
classification of syndrome by Uygur Medicine. The lymphocyte subgroup and endogenous cortisol were
usually affected by medicine. Among them, CD4/CD8, CD11b, CD11b /CD18 and CD62P heightened
gradually and lymphocyte apoptosis slowed down along with the application of hormone. CS, ACTH and
CRH decreased gradually according to the application of hormone. The research result that, all types had
149
the insidous phlegm syndromes are as follows: stethocatharsis, sticky and greasy tongue coating, such
as white and greasy fur, thick fur, yellowish and greasy tongue fur, smooth pulse, wiry pulse, deep pulse
and so on. Besides the general symptoms of asthma, patients with deficiency asthma followed with renal
deficiency syndrome such as mental exhaustion, cold body and limbs or sultriness, lassitude in loin and
legs, tinnitus and loss of memory, clear abundant urine, increased nycturia and so on, stagnation of
phlegm syndrome such as feeling of stuffiness in the chest, heaviness in head and body, numbness of the
limbs, hydroadipsia, abundant expectoration and difficult to hawk and so on, stasis syndrome such as
chest gas pains or twinge, scaly skin, pale purple tongue or dark purple tongue with ecchymosis and so
on. There was reciprocity of the three types in the severity. It was shown in the severity of asthma, mean
age, symptoms and syndromes, immune and endocrine system disturbance, and so on. Discrimination of
patients with severe asthma, deficiency asthma and abnormal sewda asthma was carried out with Fisher
method. The veracity rate was 84.1% of severe asthma, 74.1% of deficiency asthma and 66.7% of
abnormal sewda asthma. Conclusion: 1) It was found that the immunity and endocrine of patients with
asthma are indiscriminate. In severe asthma, lymphocyte subgroup and the value of its mediators such as
CD4, CD8, CD4/CD8, CD62P,CD11b, CD11b/CD18 and TNF increases obviously. That shows negative
correlation with pulmonary function. CS, ACTH and CRH decrease obviously, but the lymphocyte
apoptosis is not evidently. Therefore, in combination with the previous study, besides clinical
manifestation and pulmonary function, lymphocyte subgroup and its mediators, lymphocyte apoptosis,
endogenous cortisol and change of IgE and ECP should be monitored dynamically when severe asthma
is judged. 2) It was found that the effect of hormone therapy on immunity and endocrine is obvious. Long
use of hormone will result in side-effect that it is disadvantage for the treatment on asthma. The material
basis might be the gradually increase of CD4/CD8, CD11b, CD11b /CD18 and CD62P, slow lymphocyte
apoptosis, and gradual decrease of CS, ACTH and CRH as well. 3) The change of CD11b and
CD11b/CD18 as the relative index of infection was obvious in the onset of asthma (especially the severe
asthma). It indicates that infection closely relate to the onset of asthma. 4) There is syndrome of “insidous
phlegm” in all differentiations of symptoms and signs for classification of syndrome of asthma. No matter
asthma belongs to sthenia syndrome, deficiency syndrome or combination of sthenia syndrome and
deficiency syndrome, “phlegm” exists from the beginning to the end of asthma. “Phlegm” plays important
role in the persistence and aggravation of asthma. “No phlegm could induce asthma”. “Phlegm is the key
to asthma”. Therefore, we suggest that the classification of onset period of asthma, such as cold asthma,
heat asthma, frigiopyretic asthma and anemophlegmatic asthma, changes to be the classification of cold
phlegm, heat phlegm, frigiopyretic phlegm and anemophlegmatic phlegm. 5) In patients with deficiency
asthma, there is syndrome of kidney deficiency and stagnation of phlegm. So we propose that deficiency
asthma is really asthma of phlegm stagnancy because of deficiency of kidney. It needs further research. 6)
We discovered that there is certain reciprocity among severe asthma, deficiency asthma and abnormal
sewda asthma, which is shown by the severe condition, old mean age, overlapping of syndrome and
symptom, indiscriminate immunity and endocrine, and so on. The study explained the relationship among
immune and endocrine system disturbance, severe asthma classified by western medicine, deficiency
asthma classified by traditional Chinese medicine, abnormal sewda asthma classified by Uighur
medicine. The research is undertaking. On basis of that, we suggest that, in the treatment on asthma, we
should not only think highly of the diagnosis of asthma by western medicine and the evaluation on
patients’ condition, but also combine with differentiation of symptoms and signs and differential diagnosis
of disease, and put the entire conception of traditional medicine, the thought of “man, as a microcosm, is
connected with the macrocosm” into the whole process of diagnosis, therapy, prognosis judgment of
150
asthma to improve the diagnosis and treatment on asthma.
A-206
Study on Neuro-Endocrine-Immune disturbance of Type-2 diabetes Millitus by traditional
Uygur Medicine and Western Medicine
Mutallip Obulkasim1, Halmurat Upur 2*
1
First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region ， China;
2Traditional
Uygur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China;
*Correspondence should be addressed to Halmurat@263.net
Objective: To investigate the relationship between neuro-endocrine-immune (NEI) systems and type-2
diabetes millites (T2DM). To explore the changing mechanisms of NEI system in different fundus
changing stages in type-2 diabetes millitesy. To further explore the changing mechanisms of NEI system
in capillary complication in T2DM. To explore the changing mechanisms of NEI system of T2DM in
different abnormal body fluid syndromes, from the point of traditional Uygur Medicine (TUM). At the end,
integrate modern Western Medicine and TUM on the T-2DM, with their constituent ratio, ages, courses
and NEI systems changing characters to find out the communication of the pathogenesis, prevention and
treatment of T-2DM existed in the NEI network between the two different styled medicines. Methods:
Certainly clinical diagnosed 174 patients with T-2DM, were enrolled into the study from September, 2007
to February 2008. Strict and impartial screened all above the T-2DM patients by diagnostic standard and
exclude standard of diabetes with ADA criteria-2005 for the diagnosis of diabetes mellitus. And 33 normal
volunteer people were served as control group after physical exam proved medical fitness. Flow
cytometer technology was utilized to detect the serum level count of CD3+, CD4+and CD8+, and NK cell;
Radio-immune technology was utilized to detect the serum level of cytokines including IL-1β
(Interleukin-1β), IL-6､TNF-α (Tumor necrosis factor-α), and hormones including ACTH
(adrenocorticotrophic hormone) and CORT (cortisol) ;Liquid Chromatography was utilized to detect the
plazma level of DA (dopamine) and NE (norepinephrine), on every patient group and control group.
TOPCON IMAGEnet 2000TM system was utilized to detect the flouressain fundus angiography of
patients with T2DM. “Diagnostic method of traditional Uygur Medicine” was utilized to identify different
abnormal hilit types of patients with T2DM. Results: NEI network related indexes shows that, a significant
decrease of serum CD4+, CD8+ lymphocytes and NK cells count and CD4+/CD8+ ratio increased was
found in T-2DM groups compared to the control group (P＜
0.01); the serum level of IL-1β､IL-6 and TNF-α
increased in T-2DM compared to the control group (P＜
0.01); It was discovered that the serum level of
ACTH and CORT decreased significantly in T-2DM compared to the control group (P＜
0.01); it was
discovered that the plasma level of DA and NE increased in T-2DM group compared to the control group
(P＜
0.01). By diagnostic standard and exclude standard of diabetes with ADA criteria-2005, and detecting
by flourescein fundus angiography, 174 T2DM patients divided into three groups: non-diabetic retinopathy
group (contains 98 patients), non-proliferating retinopathy group (56 patients), and proliferating
retinopathy group (20 patients). Mean age of non-diabetic retinopathy group significantly smaller than
non-proliferating retinopathy group and proliferating retinopathy group (P＜
0.05), mean course of
non-diabetic retinopathy group significantly shorter than non-proliferating retinopathy group and
proliferating retinopathy group (P＜
0.01), but both of the above index have no difference between
non-proliferating retinopathy group and proliferating retinopathy group (P＞
0.05). Comparing between
151
different fundus groups, serum CD4+lymphocyte count in proliferating retinopathy group smaller than
non-diabetic retinopathy group (P＜
0.05); the serum level of ACTH and CORT decreased significantly in
proliferating retinopathy group smaller than non-diabetic retinopathy group (P＜
0.05). By diagnostic
standard of “Diagnostic method of traditional Uygur Medicine”, 174 T2DM patients divided into four
groups: abnormal savda group 79 patients (45.40%), abnormal khan group 39pateints (22.41%),
abnormal belghem group 35 patients (20.12%), abnormal sepra group 21 patients (12.07%). Comparing
between Uygur Medicine different abnormal hilit groups, a significant decrease of serum CD4+cells count
was found in abnormal khan group than abnormal savda group (P＜
0.01), a significant decrease of serum
NK cells count was found in abnormal savda than abnormal khan and abnormal belghem group (P＜
0.01);
the serum level of IL-1β increased in abnormal savda group than other three groups (P＜
0.05). Comparing
between abnormal savda and non-abnormal savda groups, a significant decrease of serum CD4+and NK
cells count was found in abnormal savda group than non-abnormal savda group (P＜
0.01), the serum level
of IL-1β and IL-6 increased in abnormal savda group than non-abnormal savda group (P＜
0.01), the
plasma level of NE increased in abnormal savda group than non-abnormal savda group (P＜
0.05). The
mean age is high, and mean course is long in all of the proliferating retinopathy group and abnormal
savda group type-2 diabetes millets. On diagnosing, 40% of proliferating retinopathy group belongs to
abnormal savda diabetes. Between the proliferating retinopathy group and abnormal savda group type-2
diabetes millets, lymphocytes, cytokines, hormones and mono-peptides have the same changing outputs.
Conclusion: The nerval-endocrino-immuno network is imbalanced in the patients with type-2 diabetes
milletus. The cellular immunity be weakened, humoral immunity strengthent;endocrine function
weakened and the sympathic nerve system activated in type-2 diabetes milletus NEI nerwork. Among the
different fundus group of T2DM, the proliferating retinopathy group has the highest mean age and longest
course, and has the heaviest retinal capillary complication. So, the age and course may be the risk factor
for T2DM in retinal capillary complication. It is may be the characteristic signal of type-2 diabetes milletus,
that cellular immunity weakened and endocrine secretion decreasing, would lead to capillary endothelium
proliferating and accelerate new vasculirezation on retina. Abnormal savda syndrome is the mean
syndrome in type-2 diabetes milletes, according to the constituent ratio between the different abnormal
hilit group in Uighur medicine. Comparing with other Uygur abnormal hilit group type-2 diabetes, abnormal
savda group has the most imbalanced immune system (cellular immune depressed, and cytokines
activated), and its sympathetic nerve activated mostly. Both of proliferating retinopathy T2DM and
abnormal savda T2DM, may be the same heavier stage of T2DM, so mean age high, mean course long
and pathogenetic condition severe almost in the same degree; and their immune system ambalanced,
endocrine function depressed and sympathetic nerve activated in same condition. We conclude that,
when there appears abnormal savda syndrome in type-2 diabetes milletus, and accompany with
depressed cellular immune function and decreasing endocrine secretion, we could decide that there
would be appear diabetic microvascular complication, and decide to “treat before disease”.
A-207
Study on the Phlegm-Stasis Syndrome in Complex Diseases and the Prethrombotic State
S.Abdurhim 1, Halmurat Upur2*
1
First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region ， China;
2Traditional
Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China;
*Correspondence should be addressed to Halmurat@263.net
152
Objective: To study the proportion of phlegm-stasis syndrome in complex diseases and to explore its
relations to Prethrombotic State. Methods: The expression of CD41, CD62P on platelets, the level of
plasma tissue plasminogen activator (t-PA), Plasminogen Activator Inhibitor-1（
PAI-1）
,Activated partial
thromboplastin time (APTT), Fibrinogen ( Fib), Prothrombin time ( PT) and Thrombin time (TT) were
tested by using Flow Cytometer, ELASA method and auto coagulometer. Results: 1) The proportion of
phlegm-stasis syndrome in complex disease was 76.84%, non-phlegm-stasis syndrome was 23.16%; the
proportion of phlegm syndrome in phlegm-stasis syndrome was 24.66%, stasis syndrome was 19.18%,
stagnation of phlegm and stasis syndrome was 56.16%. 2) ①
Compared to control group, there was no
significant difference on the expression of platelet CD41 and TT in phlegm-stasis syndrome
group(phlegm syndrome, stasis syndrome, stagnation of phlegm and stasis syndrome groups )(P>0.05);
however the expression of platelet CD62P was higher (P<0.01) in phlegm-stasis syndrome group(phlegm
syndrome, stasis syndrome, stagnation of phlegm and stasis syndrome groups); the level of plasma t-PA
was lower(P<0.05) in phlegm-stasis syndrome group (phlegm syndrome group P>0.05, stasis syndrome,
stagnation of phlegm and stasis syndrome groups P<0.05); the level of plasma PAI-1 was higher(P<0.05)
in phlegm-stasis syndrome group (phlegm syndrome, stasis syndrome groups P<0.05, stagnation of
phlegm and stasis syndrome group P<0.01); the level of plasma Fib was higher(P<0.01) in
phlegm-stasis syndrome group (phlegm syndrome, stasis syndrome, stagnation of phlegm and stasis
syndrome groups P<0.01); PT, APTT was shortened(P<0.01) in phlegm-stasis syndrome group (phlegm
syndrome, stasis syndrome, stagnation of phlegm and stasis syndrome groups P<0.01). ②
Compared to
non-phlegm-stasis syndrome group, the expression of platelet CD62P was higher(P<0.01)( phlegm
syndrome group P>0.05, stasis syndrome group P<0.05, stagnation of phlegm and stasis syndrome
group P<0.01), and the level of plasma Fib of phlegm-stasis syndrome group were higher(P<0.01)(
phlegm syndrome group P>0.05, stasis syndrome group P<0.05, stagnation of phlegm and stasis
syndrome group P<0.01), although the expression of platelet CD41, the level of plasma t-PA, PAI-1, PT,
TT and APTT were insignificantly different between two syndrome(P>0.05), each the items above had a
certain gradient on phlegm syndrome, stasis syndrome, stagnation of phlegm and stasis syndrome and
non-phlegm-stasis syndrome groups, and the changes were the most obvious in stagnation of phlegm
and stasis syndrome groups. Conclusion: 1) phlegm-stasis syndrome is more common and is one of the
important syndrome types of complex disease; stagnation of phlegm and stasis syndrome are more
prevalent than phlegm and stasis, stagnation of phlegm and stasis are the common result of phlegm and
stasis. 2) There is a different degree of prothrombotic state in phlegm-stasis syndrome of complex
disease; the changes of prethrombotic state including the expression of platelet CD62P, the level of
plasma t-PA, PAI-1, Fib, PT and APTT are more obvious in phlegm-stasis syndrome than that of the
non-phlegm-stasis syndrome.
A-208
Study on relationship between TCM syndrome differentiation and NeuroendocrineImmune-Netwok in Type-2 diabetes
Palidan Wushuer, Halmurat Upur 2*
1 Urumqi
City Traditional Chinese Medicine Hospital, 2Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi
830011, Xinjiang Uighur Autonomous Region, China; *Correspondence should be addressed to Halmurat@263.net
153
Objective: To explore the relationship between TCM syndrome differentiation and
neuroendocrine-immune-netwok in Type 2 Diabetes. Methods: Collect the Uighur and Han patients of
type 2 diabetes from Traditional Chinese Medicine Hospital in Urumqi Xinjiang from November 2007 to
January 2008 of 105 cases, divided them into different groups according to TCM syndrome differentiation
and analysis the relevant indicators. In the normal control group there are 30 cases without lung, liver,
kidney and blood diseases, without autoimmune diseases and thyroid dysfunction. The Serum
auxiliary/suppressor T cell ratio (CD3+ CD4+/CD3+ CD8+) and natural killer (NK) cells, Serum
interleukin-1β (IL-1β), IL-6, tumor necrosis factor(TNF-α), and cortisol (CORT), adrenocorticotropic
hormone (ACTH), plasma dopamine (DA) and Norepinephrine (NE) were tested by using Flow Cytometer,
radioimmunoassay method and liquid chromatography. Results: Among the total clinical cases, the ratio
of tan zhuo zhong zu patients was 43.7%，
the ratio of yin xu re sheng patients was 20.0%，
the ratio of qi yin
liang xu patients was 19.3%, the ratio of xu yu mai luo and yin yang liang xu xue yu shui ting patients was
17.0%. Compared to control group，
the level of CD4、
CD8、
NK、ACTH、
CORT of type 2 diabetes were
decreased (P<0.01); The level of IL-1β、
IL-6 and TNF-αwere increased (P<0.01); the level of NE、
DA were
increased (P<0.05); there were no significant changes in CD4/CD8 between two groups (P>0.05). The
level of DA、
NE were increased (P<0.01) in TCM syndrome differentiation, the gradient changes of DA、
NE
was xu yu mai luo and yin yang liang xu xue yu shui ting＞
tan zhuo zhong zu＞
qi yin liang xu＞
yin xu re
sheng；
the level of CORT was decreased (P＜
0.05), the gradient change of CORT was: qi yin liang xu＞
tan
zhuo zhong zu＞
xu yu mai luo and yin yang liang xu xue yu shui ting＞
yin xu re sheng. Conclusion: 1) There
was a disordered status on Nerve - endocrine - immune network in type 2 diabetes. 2) The level of CORT
decreased might be one of the material basis of xu zheng and zheng qi bu zu, The level of DA、
NE
increased might be related to shi zheng and tan yu jiao zu. 3) We propose to take CORT、
DA、
NE as part of
the micro-indicators on TCM syndrome differentiation.
A-209
Study on the relationship between Qi asthenia phlegm-stasis syndrome of the malignant
tumor and Immunity Cell and Hormone secretion
Wang Qiaolin1, Du Yeqin 1, Halmurat Upur 2*
1Tumor
Center of the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China;2Traditional Uighur Medicine
Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China; *Correspondence should be
addressed to Halmurat@263.net
Objective: To analyse the relationship between Qi asthenia phlegm-stasis syndrome of the malignant
tumor patients and Immunity Cell and Hormone secretion. Method: The flow cytometry technology and
radio-immune technology were utilized to detect patients' serum level of CD3+、
CD4+、
CD8+、
NK
cell、
IL-1β、
IL-6、
ACTH、
CORT、
TNF-α of 41 cases of malignant tumour with Qi asthenia and phlegm-stasis
syndrome and 42 cases of malignant tumour with non-Qi asthenia and phlegm-stasis syndrome. Result:1)
according to Traditional Chinese Medicine dialectics type, the Qi asthenia and phlegm-stasis syndrome
was 49.39% ,the non-Qi asthenia and phlegm-stasis syndrome group was 50.61%. 2) ①
In Qi asthenia and
phlegm-stasis syndrome group and all the non-Qi asthenia and phlegm-stasis syndrome group,
CD3+、
CD4+、
CD4+/CD8+、
NK went down, and CD8+ went up .There is statistical difference compared
with normal group(P＜
0.01 or P＜
0.05), while compared with the Qi asthenia and phlegm-stasis syndrome
154
group with the non-Qi asthenia and phlegm-stasis syndrome group in CD3+、
CD4+, there is no statistical
significance (P＜
0.05). ②
IL-1β and IL-6 in Qi asthenia and phlegm-stasis syndrome group and the non-Qi
asthenia and phlegm-stasis syndrome group decreased significantly compared with normal group (P＜
0.01
or P＜
0.05), but there is no obvious difference in both groups(P＞
0.05). ③
TNF-α decreased in Qi asthenia
and phlegm-stasis syndrome group and the non-Qi asthenia and phlegm-stasis syndrome group.
Compared with normal group, there is no statistical significance (P>0.05). ④
ACTH and CORT went up in Qi
asthenia and phlegm-stasis syndrome group and the non-Qi asthenia and phlegm-stasis syndrome group
compared with normal group (P<0.05) (P<0.01), the two groups had no obvious difference (P＞
0.05).
Conclusion: 1) Qi asthenia and phlegm-stasis syndrome occupied greater proportion in malignant
tumour of TCM dialectical type, so Qi asthenia and phlegm-stasis syndrome was one of the important
syndrome types of malignant tumour. 2) The change of Immunity Cell and Hormone secretion exist in Qi
asthenia and phlegm-stasis syndrome group and the non-Qi asthenia and phlegm-stasis syndrome
group, especially in Qi asthenia and phlegm-stasis syndrome group was more outstanding, in which the
change was primarily in CD3+、
CD4+、
CD8+、
NK、
IL-1β、
ACTH.
A-210
The clinical observation of the fixation in the treatment of acute ankle sprain in
Traditional Uygur Medicine
Jin Hao1, Lv Faming1*
1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi
830011, Xinjiang Uighur Autonomous Region, China;
*Correspondence should be addressed to lvgangxj@163.com
Objective: To explore the traditional Uygur Medicine Efficacy of the treatment of acute ankle sprains, and
evaluate of its clinical advantages and characteristics. Method: According to the Western and TCM
Designate diagnostic criteria, by collection, 94 cases of acute ankle sprain were all from orthopedic clinic
and orthopedic emergency were randomized, in which 37 cases called observation group using
Traditional Uygur external fixation and 57 cases with cast immobilization were called the control group.
Two sets of initial therapy with conventional "RICE" method and oral blood circulation of the san qi pill
were used. External fixation of the observation group and control group changed once a week, one week
course of treatment and continuous observation of the two courses, TCM syndrome score and the
AOFAS score, VAS score, and ankle swelling as the efficacy evaluation. Results: there were no
significant Difference between the two groups in gender, age, age distribution, the tribes, (P>0.05), the
AOFAS score of the two groups were statistically significant compared with the before treatment
(P<0.05), there were no significant difference one week and two weeks after the treatment for the point in
time (P>0.05). TCM syndrome score were compared statistically different (P<0.05). The difference were
statistically significant in the VAS pain product and swollen joints before and after treatment
(P<0.05).Conclusion: By early using the traditional Uygur Fixation for the treatment of acute ankle
sprain, we can clearly achieve the anti-inflammatory swelling, relieve pain in certain geographical areas
and can effectively replace the cast immobilization.
A-211
155
The Clinical Research of Acupuncture Combined with Auricular Acupuncture for Patients
with Acute Exacerbation of COPD to Improve their Quality of Life and Pulmonary
Function
Cao Lin1, Liu Zhiyan1
1
Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，
Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China
Objective: To observe the influence of life quality and the improvement of pulmonary function with
acupuncture combined with auricular acupuncture therapy on patients with acute exacerbation of COPD.
Methods: 60 patients with acute exacerbation of COPD were randomly divided into four groups:
acupuncture combined with auricular acupuncture group, acupuncture group (acupuncture combined with
simulate auricular acupuncture), auricular acupuncture group (simulate acupuncture combined with
auricular acupuncture), control group (simulate acupuncture combined simulate auricular
acupuncture).Four groups received conventional treatment as basis combined with acupuncture,
auricular acupuncture, simulated acupuncture, simulated auricular acupuncture. After two courses (20
days) of treatment, observe FEV1, FEV1/FVC ratio of pulmonary function and CAT, MRC, as well as
traditional Chinese medicine symptom scale quality of life scores of the four groups of patients before and
after treatment. Results: The lung function: There was statistically significant (P<0.05) of patients with
pulmonary function in four groups before and after treatment. acupuncture combined with auricular
acupuncture group’s FEV1、
FEV1/FVC ratio improved compared with before treatment, the improvement
of pulmonary function was better than the other three groups; CAT scales reflect the quality of life score
decreased significantly compared with before treatment, acupuncture combined with auricular
acupuncture group was better than the acupuncture group and auricular acupuncture group, and was
statistically significant compared with control group (P<0.05). Conclusion: Acupuncture combined with
auricular acupuncture for treating acute exacerbation of COPD patients with the pattern of phlegm
obstructed in lung, which is effective in the clinical symptoms, especially the improvement of airflow
limitation as well as difficulty in breathing. To compare the four groups with each other, we suggest that
acupuncture, auricular acupuncture can treat COPD, the combination of their two is better, and patients’
quality of life are also improved.
A-212
The correlation of TCM syndrome with the BODE index and quality of life for patients with
COPD at stable phase
Zhou Wei 1, Mao Bing 1*
1Huaxi
hospital of Sichuan university of combination of TCM and western medicine department, 610041, Chengdu, China
Objective: To study the syndrome of TCM (Traditional Chinese Medicine) and relevant indexes of
conventional Western Medicine for patients with COPD (chronic obstructive pulmonary disease) at stable
phase, and further explore the trend of change of the deficiency syndrome of TCM, and the correlation of
each syndrome with the BODE index (body mass index, airflow obstruction, dyspnea, and exercise
capacity index) and quality of life. It was anticipated that this study could provide evidence-based
information for pattern differentiation, and also promote the combination of TCM and conventional
156
Western medicine in the treatment of COPD at the stable phase. Methods: Based on relevant literature
and diagnostic criterion of TCM, five groups of symptoms of TCM about stable COPD were established,
which are Lung Qi deficiency , Lung and Spleen Qi deficiency , Lung and Kidney Qi deficiency, Lung,
Spleen and Kidney Qi deficiency, and deficiency of both Qi and Yin. 300 cases which are up to the
standard were differentiated to 5 groups by the symptoms. Some basic details about the patients were
recorded, such as course of disease, time of acute exacerbation every year, weight and height. Also
BODE index and COPD assessment test were detected. And then statistic and analyses the differences
of the indexes among groups, and the relationship between the BODE index and the CAT (COPD
assessment test) score. Results: ①
The course of disease in Lung Qi deficiency group was shorter than
those in Lung and Kidney Qi deficiency group, Lung, Spleen and Kidney Qi deficiency group, deficiency of
both Qi and Yin group (P<0.05); the times of acute exacerbation every year in Lung Qi deficiency group
were less than those of the rest four groups (P<0.05).②
The BMI of the patients in Lung Qi deficiency group
was higher than that in Lung and Spleen Qi deficiency group, Lung, Spleen and Kidney Qi deficiency
group, deficiency of both Qi and Yin (P<0.05); the BMI of the patients in Lung and Kidney Qi deficiency
group was higher than that in Lung, Spleen and Kidney Qi deficiency group, deficiency of both Qi and Yin
(P<0.05). ③
The degree of airflow obstruction of patients in Lung Qi deficiency group was mainly
concentrated in Ⅱ
stage, the degree of airflow obstruction of patients in Lung and Spleen Qi deficiency
group was mainly concentrated in Ⅲstage, the degree of airflow obstruction of patients in Lung, Spleen
and Kidney Qi deficiency group, deficiency of both Qi and Yin group were mainly concentrated in Ⅲ
、
Ⅳ
stage.
The order of FEV1% from high to low was Lung Qi deficiency/Lung and Spleen Qi deficiency/Lung and
Kidney Qi deficiency/Lung, Spleen and Kidney Qi deficiency(P<0.05). ④
the patients' degree of dyspnea in
Lung Qi deficiency group was lighter than that in the rest four groups(P<0.05); the patients' degree of
dyspnea in Lung and Spleen Qi deficiency group was lighter than which in Lung, Spleen and Kidney Qi
deficiency group and the deficiency of both Qi and Yin group (P<0.05); the patients' degree of dyspnea in
Lung and Kidney Qi deficiency group was lighter than that in Lung, Spleen and Kidney Qi deficiency
group(P<0.05). ⑤
The 6MWD of patients in Lung Qi deficiency group was longer than that in the other four
groups(P<0.05); the 6MWD of patients in Lung and Spleen Qi deficiency group was longer than which in
Lung, Spleen and Kidney Qi deficiency group and deficiency of both Qi and Yin group(P<0.05); the 6MWD
of patients in Lung and Kidney Qi deficiency group was longer than that in Lung, Spleen and Kidney Qi
deficiency group(P<0.05); ⑥
The patients' BODE index and CAT score in Lung Qi deficiency group is lower
than that in the other four groups(P<0.05); the patients' BODE index and CAT score in the Lung and
Spleen Qi deficiency group is lower than that in Lung, Spleen and Kidney Qi deficiency and deficiency of
both Qi and Yin group(P<0.05); the patients' BODE index and CAT score in Lung and Kidney Qi deficiency
group is lower than that in the lung-qi, spleen-qi and kidney deficiency group (P<0.05).⑦
The patients' CAT
score in Lung and Kidney Qi deficiency group is lower than that in deficiency of both Qi and Yin group; The
CAT score of patients in Lung, Spleen and Kidney Qi deficiency group is lower than that in deficiency of
both Qi and Yin group(P<0.05) ⑧
The BODE index were dramatically relative with CAT score.
Conclusions: These findings suggest that with the progress of COPD, the functions of multiple Zang-Fu
organs could ultimately be affected. The symptom type of TCM for the COPD patients at stable phase
may vary from Lung Qi deficiency to Lung and Spleen Qi deficiency, or to Lung and Kidney Qi deficiency,
and even lung-qi, Lung, Spleen and Kidney Qi deficiency. Additionally, the BODE index and CAT score
become increasingly high. The BMI of patients with Spleen-Qi deficiency is lower than that without
Spleen-Qi deficiency. The lung function, the degree of dyspnea and the 6MWD of patients with Kidney-Qi
deficiency are lower than that without Kidney-Qi deficiency. As for the BODE index and CAT score,
157
patients with Yin deficiency are superior to that without Yin deficiency. The BODE index score were
positively correlated with CAT score.
A-213
The effect of Tanreqing injection on lipopolysaccharide-induced airway inflammation,
mucus hypersecretion in rats and their own dose-response relationship
Zhou Yunqing 1, Mao Bing 1*
1Huaxi
hospital of Sichuan university of combination of TCM and western medicine department, 610041, Chengdu, China
Objective: The aim of this study was to observe the role of lipopolysaccharide (LPS)-induced airway
inflammation and mucus hypersecretion in rats, and to investigate the effect of Tanreqing injection on
airway inflammation, mucus hypersecretion and their own dose-response relationship. Methods:
Forty-eight male specific-pathogen-free Sprague-Dawley rats were randomly divided into four groups,
with 12 rats in each group: control group, LPS group, and Tanreqing groups (at doses of 2.8 and 5.6
ml/kg/24h, respectively). Control rats were given a 120μl saline through intratracheal instillation. An equal
volume of LPS was instilled intratracheally to induce airway inflammation and mucus hypersecretion.
Tanreqing injection at 2.8 and 5.6ml/kg/24h was given with an intraperitoneal injection at 1h prior to LPS
administration and then once every 24h. Rats from the control and LPS groups received 5.6ml/kg/24h
saline instead of Tanreqing injection. Rats were randomly sacrificed by exsanguination at 24h, 48h, and
96h after LPS challenge. The bronchoalveolar lavage fluid (BALF) and the right lung were collected. The
number of total cells and neutrophils in BALF were determined. The levels of inflammatory cytokines
interleukin-1β (IL-1β), CINC-1, and tumor necrosis factor-α (TNF-α) in BALF were measured using
ELISA. The middle lobe of the right lung was stained with hematoxylin-eosin (H&E), and the histological
change was examined. BALF was analyzed for total protein using the BCA method and Muc5ac
quantification using ELISA. Additionally, airway mucus hypersecretion was also evaluated with Alcian
blue/Periodic acid-schiff (AB/PAS) staining, immunohistochemistry, and real time-PCR. Results: In the
control group, there were no significant changes in terms of the number of inflammatory cells and the
levels of inflammatory cytokines in BALF, the histological change, total protein and Muc5ac quantification
in BALF, AB/PAS staining area, and Muc5ac expression. Compared with the control group, LPS induced
significant increase in above markers. The inflammatory markers induced by LPS were peaked on 24h,
decreased on 48h, and further decreased on 96h. The airway mucus hypersecretion induced by LPS
were increased on 24h, further enhanced on 48h, and peaked on 96h. Treatment with Tanreqing injection
significantly reduced the number of the LPS-induced inflammatory cells, the levels of inflammatory
cytokines in BALF, histopathological change, total protein in BALF, AB/PAS staining area, and Muc5ac
expression. Furthermore, high-dose of Tanreqing injection shows increasing benefits over low dose, but
the difference was not statistically significant. Conclusions: The LPS-induced airway inflammation were
peaked on 24h, decreased on 48h, and further markedly decreased on 96h. The LPS-induced airway
mucus hypersecretion were increased on 24h, further enhanced on 48h, and peaked on 96h. The present
results suggest that Tanreqing injection is endowed with anti-inflammatory properties and can effectively
inhibit the airway mucus hypersecretion. In addition, Tanreqing injection inhibits the airway inflammation
and mucus hypersecretion in a dose-dependent trend. However, their own dose-response relationship
and involved molecular mechanism of Tanreqing injection have not been elucidated.
158
A-214
The Modern Medicine and Traditional
neuro-endocrine-immune in malignancies
Uighur
Medicine
research
of
Zhang Li 1, Halmurat Upur 2*
1Tumor
Center of the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China; 2Traditional Uighur Medicine
Department, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China; *Correspondence should
be addressed to Halmurat@263.net
Objective: 1) To investigate the disorder of neuro-endocrine-immune network in patients with cancer and
its clinical significance. 2) To investigate clinical types of malignancy in Traditional Uighur Medicine and
the disorder of neuro-endocrine-immune network. 3) In order to investigate the alteration of some indexes
about neuro-endocrine-immune in different model types, the abnormal savda syndrome transplanted
tumor model was established on the basis of the abnormal savda syndrome model. In order to research
further the relevance between the abnormal savda syndrome tumour and the disorder of
neuro-endocrine-immune, the morphological and ultrastructural alteration of hypothalamus
-pituitary-adrenal axis was observed. Methods: 1)102 patients with histological proven cancer were
enrolled in the study. Radio-immune technology and flow cytometer technology were utilized to detect
blood levelofCD3+,CD4+and CD8+, and cytokines including IL-1β, IL-6,TNF-α,ACTH, CORT and DA as
well as NE; And meanwhile analyses were performed on the basis gender, pathology, metastasis status,
and performance status and treatment pattern. 30 normal people were served as control group. 2)
Radio-immune technology and flow cytometer technology were utilized to detect the serum level of
CD3+、
CD4+ and CD8+, and cytokines including IL-1β、
IL-6、
TNF-α、
ACTH、
CORT、
DA and NE. 3) 120 ICR
mouse were divided randomly into four model groups namely group control, group tumour, group
abnormal savda syndrome model and abnormal savda syndrome tumour model. After the abnormal
savda syndrome model was established, S180 ascites tumour cells were transplanted subcutaneously
into the mouse thigh, then macroscopic tumour mass were formed. The serum level of IL-1β, IL-6, TNF-α,
ACTH and CORT were examined by ELISA. The structural change of hypothalamus, pituitary and
adrenal were observed via using microscope and electronic microscope to calculate the rate of
transplantation and to weigh the tumour mass. Results: 1) A significant decrease of CD3+, CD4+
lymphocytes count, CD4+/CD8+ and a significant increase of CD8+lymphocyteswere found in almost all
the cancer patients compared with the control group(P<0.05 or P<0.01). Cancer groups had lower
IL-1βand TNF-α level, l but falling in lung cancer group and head and neck group were significant
(P<0·05). Various cancer types had lower NK level than in normal group (P<0·05). The serum CORT level
increased only in gastrointestinal cancer patients (P<0.05); The ACTH level had no difference among all
groups. Both serum DA and NE level were increased in almost all cancer groups compared with the
normal group (P<0·05 or P<0·01). 2) Decrease ofCD3+lymphocytes in patients treated by chemotherapy
and irradiation therapy vs non-CT/RT patients were significant (P<0·05). In RT group the higher level
ofCD8+and lowerCD4+/CD8+was significant than non-CT/RT (P<0·05). The level of IL-1β and Il-6 in CT
group was higher than RT group (P<0·05); but the latter had higher TNF-α level (P<0·05). (3)In female
patients, CD3+and CD8+lymphocytes, IL-1βand TNF-α were increased significantly than in male patients
(P<0·05). (4)Our data presented that IL-6 was significantly increased in patients with cancer and
mestastic ones (P<0·05). (5)The poor performance status patients had higher CORT and TNF-α
(P<0·05); The level ofCD4+/CD8+in patients in the late stage was significantly lower than in the early
159
stage patients (P<0.05). 2)①Patients with Abnormal Savda Syndrome cancer occupied 72% in all
patients; non-abnormal Savda Syndrome cancer was 28%. ②A significant decrease of total and CD3+
and CD4+ lymphocytes count was found in abnormal savda syndrome cancer patients compared to both
the control group(P＜
0.01)and non-Abnormal Savda Syndrome cancer(P＜
0.05). A significant increase of,
the abnormal savda syndrome and non-abnormal savda syndrome had higher CD8+ compared with the
control group(P＜
0.05), the former was more obvious. A significant decrease of CD4+/ CD8+ was found out
in both two tumor groups(P＜
0.01),but the abnormal savda syndrome was little obvious. The two tumor
groups had lower level NK (P＜
0.05), but no significant difference between them (P＞
0.05). ③Two cancer
groups had lower IL-1β level, but falling in non-abnormal Savda Syndrome cancer group was significant
(P＜
0.05). Compared to the control group, IL-6 and ACTH was significantly increased in patients with in
abnormal savda syndrome cancer and non-abnormal savda syndrome cancer, but no difference between
them. TNF-α was increased significantly only in Abnormal Savda Syndrome cancer patients (P＜
0.05). The
serum level of CORT in the abnormal savda syndrome cancer present significant increasing than
non-Abnormal Savda syndrome cancer (P＜
0.05). ④A significant increasing of DA in serum in both
abnormal savda syndrome cancer and non-abnormal savda syndrome cancer(P＜
0.01). The serum level of
NE was increased significantly in non-abnormal savda syndrome cancer compared with abnormal savda
syndrome cancer (P＜
0.05). 3) ① In the state of abnormal savda syndrome, the rate of tumor
transplantation was increased significantly than the normal state, respectively 100% and 63.3% (P＜
0.01);
and the weight of tumor mass was higher in former than in later (P＜
0.01). ②Under microscope,
compared with the normal group, cells of the hypothalamus were characterized by the glial cells
hyperplasia, neurocells edema, vacuolus degeneration in all three model groups, but the group abnormal
savda syndrome tumor model presented more sever than other two groups. Pituitary cells hyperplasia
and vascular dilation were similar in three model groups. ③All the three model groups had HPAA
ultrastructural abnormality including cell nucleus malformation, perinuclear cisterna enlarged,
heterochromatin aggravation, cell organelle falling and so on, especially the abnormal savda syndrome
tumor model was the most serious, and the abnormal savda syndrome group was second one, the tumor
model was relatively mold. ④Composed with normal group, both abnormal savda syndrome tumor
model and abnormal savda syndrome group the serum level of ACTH were increased and CORT
deceased, IL-6 also increased (P＜
0.05), but no difference between them(P＞
0.05). TNF-α was raised in
abnormal savda syndrome group than in tumor group (P＜
0.05). There was no different between four
groups to IL-1β. Conclusion: 1) The disturbance of neuro-endocrine-immune network in patients with
cancer is characterized by increase of the serum level of TNF-α, IL-6, CORT, DA and NE and decrease of
total CD3+, CD4+ and CD4+/CD8+ lymphocytes; Moreover it is related to the gender, pathology,
mestastic status and treatment type; not related to the PS. 2) Abnormal Savda syndrome body fluid
facilitated tumour development, a phenomenon that might be related to the neuroendocrine-immune
disorder. HPAA damages in abnormal Savda syndrome tumour model were more severe than the other
experimental groups, suggesting that abnormal Savda syndrome body fluid and the tumour might have
a synergistic effect on HPAA damages. The disturbance of the neuroendocrine-immune axis in
abnormal Savda syndrome tumours was more severe as shown by the morphological data.
A-215
The Observation of Curative on Extracorporeal High-Frequency Hyperthermia Combined
with Yangzhengxiaoji Decoction ’s Treatment of Advanced Non-small-cell Lung Cancer
160
Wu Shanshan 1, Zhang Hongliang 1
1
Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomoes Region, China; *Correspondence should be addressed to zhanghongliang@csco.org.cn
Objective: Using pilot study method of the clinical non randomized controlled, patients with advanced
non-small cell lung cancer(NSCLC) were treated with prospective study of Integrative Chinese and
Western Medicine, whose ages were between 30-85 years old. To observe the combined treatment
efficacy and advantages of quality of life. Methods: 92 advanced NSCLC patients who visited the TCM
hospital of Xinjiang Autonomous Region and the TCM hospital of Urumqi city were divided into two
groups by a randomized controlled pilot study medthod. The test group: extracorporeal high-frequency
hyperthermia combined with Yangzhengxiaoji decoction; the control group: simple Yangzhengxiaoji
decoction, 46 cases in each of group. In the four weeks of treatment, we recorded every patients’
baseline indexes, tumor lesions change, KPS score, symptom score and quality of life score. Results: Of
the 92 patients, 87 patients completed test, 5 patients were out of the groups. The two groups had same
levels in tumour body stable rate and reduced TCM hospital syndrome evaluation(P>0.05). The treatmeat
group was better than the control group on improving patients with KPS score. Analysing the quality of life,
functional area: two groups of patients have same improved degree of role function, emotional function,
social function, general health status of the functions(P>0.05); The treatment group patients were
improved with the physical function, cognitive function, and the improved physical function is better than
that of control group (P<0.05); improvement of the cognitive function compared with the control group had
no significant.(P>0.05); Symptoms: the two groups had the improved patient’s symptoms in the side of
pain, dyspnea and lung specific symptoms ( including shortness of breath, cough, hemoptysis, pain ). The
curantive effects in treatment group was better than that in control group(P<0.05); the two groups had
improved the patients’ fatigue symptom, but none of obvious difference(P>0.05). The two groups
increased the cost of patients, and the treatment group was significant, but none of obvious
differences(P>0.05). Conclusion: (1) External high frequency thermotherapy combined with
Yangzhengxiaoji Decoction method could improved patients’ physical condition, peoples’ quality of life by
the improvement of pain, dyspnea symptoms; (2)This program could be one of the treatment scheme
selections on non-small-cell lung cancer.
A-216
The relationship between Bronchial asthma TCM syndrome differentiation and their
NE、
DA、
5-HT changes
You Lina 1, Halmurat Upur 2*
1
Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous RegionChina; 2Traditional
Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China;
*Correspondence should be addressed to Halmurat@263.net
Objective: To investigate the change between bronchial asthma patients' plasma monoamine
neurotransmitter and their TCM syndrome differentiation. Methods: Randomly collect 84 cases of
bronchial asthma patients from the respiration department in the Affiliated Traditional Chinese Medicine
hospital of Xinjiang Medical University. Divided them into different groups according to TCM syndrome
differentiation. The contents of plasma monoamine neurotransmitter were measured by high
161
perfonmence liquid chromatography (HPLC), such as NE、
DA and 5-HT.46 normal people were served as
control group. Results: In total clinical cases, the ratio of kidney deficiency and stagnation of phlegm was
46%, the non- kidney deficiency and stagnation of phlegm was 54%(contain the ratio of cold phlegm was
21%, the ratio of heat phlegm was 15%, the ratio of frigiopyretic phlegm was 7%, the ratio of
anemophlegmatic phlegm was 11%).The kidney deficiency and stagnation of phlegm mean age is greater
than the non- kidney deficiency and stagnation of phlegm. And the two of group didn't present statistical
significance in gender (p>0.05).The content of NE and DA in bronchial asthma patients was higher than
the normal control group (p<0.05). Inter-compared with kidney deficiency and stagnation of phlegm and
non- kidney deficiency and stagnation of phlegm and control group, the content of NE and DA in three
groups present statistical significance. compared to the normal control group, NE and DA was
significantly decreased in kidney deficiency and stagnation of phlegm(p<0.05),but in non-kidney
deficiency and stagnation of phlegm, the content of NE and DA didn't present statistical
significance(p>0.05).A significant increase of NE and DA was found in non-kidney deficiency and
stagnation compared to kidney deficiency and stagnation(p<0.05),two groups had no statistical
significance in 5-HT(p>0.05). Conclusions: 1) Bronchial asthma patients' plasma monoamine
neurotransmitter are disordered, asthma can cause the increase of neurotransmitter, or nervous reflex
could aggravate symptoms of asthma. 2) “The same disease different syndrome, the different syndrome
different principle”. Kidney deficiency and stagnation of phlegm was the most common type of bronchial
asthma. There is variability in the release of neurotransmitter because of the difference of TCM syndrome
differentiation. The lower content of NE and DA in bronchial asthma patients of kidney deficiency and
stagnation of phlegm, it is maybe excitability of central nervous system to lower, so the release of
monoamine neurotransmitter to be depression. This is maybe a part of biology foundation of “deficiency in
origin” in asthma of kidney deficiency and stagnation of phlegm.
A-217
The relationship between tumor patients’ plasma monoamine neurotransmitter changes
and their Traditional Uighur Medicine syndromes
Chen Qingying1, Du Yeqin 1, Halmurat Upur 2*
1
Affiliated Cancer Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region，China;
2Traditional
Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China;
*Correspondence should be addressed to Halmurat@263.net
Objective: To determine the relation between tumor patients’ plasma monoamine neurotransmitter and
their Traditional Uighur Medicine types. Methods: Grouped the patients according to their Traditional
Uighur Medicine syndromes. The contents of plasma monoamine neurotransmitter were measured by
high perfonmence liquid chromatography (HPLC), such as NE, DA, 5-HT and DOPAC et al. Results:
Compared with venign tumour group, the percentage of Malignancy abnormal savda syndrome was
markedly highly than the other three abnormal Traditional Uighur Medicine syndrome. The contents of
plasma 5-HT have significantly difference between malignancy and venign tumour patients. While the
contents of plasma NE and the levels of NE/DA are significantly different between abnormal savda
syndrome patients and the other Traditional Uighur Medicine syndromes. Conclusion: The above results
suggest that the content change of NE and the level of NE/DA may be indexes to diagnose the
malignancy tumour belonging to abnormal savda syndrome. In a word, the plasma monoamine
162
neurotransmitters changes and the imbalance among them are related to the Traditional Uighur Medicine
syndromes.
A-218
The study between IFN-γ methylation and Chinese medicine syndrome type in subsets of
allergic rhinitis patients between Uighur and Han in Ili of Xinjiang
Zhang Qingquan 1, Gao Li 1*
1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi
830011, Xinjiang Uighur Autonomous Region, China;
*Correspondence should be addressed to Xjmugaoli@sina.com
Objective: To explore the expressions of IFN-γ gene methylation and Chinese medicine syndrome type
between Uygur and Han population in patients with allergic rhinitis in Ili of Xinjiang. Through epidemiology
investigation of allergic rhinitis get distribution rule of Chinese medicine syndrome type. To analysis the
correlation between serum IL-4 , IFN-γ and IgE level and nasal mucosa eosinophilic. Methods: AR
patients and normal group were randomly surveyed from the crowds through allergic rhinitis
epidemiological survey in Ili of Xinjiang. To extract genomic DNA from peripheral venous blood and
modified DNA by bisulfit emodification and adopt methylation specificity polymerase chain reaction(MSP)
for the analysis of methylation state.Through double-antibody Enzyme-linked Immunosorbent Assay
(ELISA), 59 cases Uygur AR patients, 50 patients Han’s AR serum IL-4 and IFN-γ levels were analyzed,
pathology smear was observed and nasal smear mucosa eosinophil was counted at high-power
microscope. Results: The positive rates of IFN-γ gene methylation of Han healthy control
group(16.9%)were lower than in Uygur healthy control group(1.7%)(P<0.05). IFN-γ hemimethylation rate
of Han healthy control group(96.6%) was higher than in Uygur healthy control group(79.7%)(P<0.01). It
not only had no significant differences between Han AR patients(8.5%) and control group(1.7%) but also
between Uygur AR patients(18.6%) and control group (16.9%) in positive rate of IFN-γ gene methylation
(P>0.05). There were no significant differences between Uygur AR patients (18.6%) and Han (8.5%) of
IFN-γ gene methylation (P>0.05). The highest Chinese Medicine syndrome type of deficiency of lung
qi(69.01%),followed by deficiency of spleen qi type(13.22%)，
syndrome of lung heat(9.51%),syndrome of
deficiency of kidney(8.26%). IL-4 and IgE levels and nasal eosinophil counts were positively correlated in
Xinjiang Uygur and Han population in patients with AR (P<0.01), IFN-γ levels and nasal eosinophil count
was negatively correlated (P<0.01); Conclusion: The positive rates of IFN-γ gene methylation of Han
healthy control group were lower than in Uygur healthy control group, but it had no significant differences
between AR patients and control group, it will provide us with many benefits to our future research. In
Chinese medicine syndrome type, deficiency syndromes in lung type higher than the other three types,
Climate and environment have a certain influence on AR. For Uygur and Han people, excess secretion of
serum IL-4, IgE and inhibition secretion of IFN-γ were important factors in allergic rhinitis nasal eosinophil
counts production.
A-219
The study of the improvement of clinical effect about “Reinforcing Qi and Activating
Blood” in Stable Chronic Obstructive Pulmonary Disease (Qi Deficiency and Blood Stasis
Syndrome)
163
Min Jie 1, Mao Bing 1*
1Huaxi
hospital of Sichuan university of combination of TCM and western medicine department, 610041, Chengdu, China
Objective: Under the direction of “Reinforcing Qi and Activating Blood”, we studied the TCM clinical effect
on patients with stable chronic obstructive pulmonary disease (Qi Deficiency and Blood Stasis
Syndrome), who were administered with Yu-Ping-Feng capsules and Fu-Fang-Dan-Shen tablets. We
finally assessed the improvement of TCM clinical symptoms, evaluated the effect of TCM syndrome, and
determined the TCM clinical curative effect through comparing with experimental datas and the
end-points. Method: A randomized controlled trial (RCT) was designed. Fifty outpatients with stable
COPD of Qi Deficiency and Blood Stasis Syndrome were randomly and averagely allocated to control
group and treatment group. Patients in the study group were given both health education and the
treatment of Yu-Ping-Feng capsules and Fu-Fang-Dan-Shen tablets for 3 months, while patients in the
control group were only given health education for 3 months, then all patients were followed-up for 12
months. We calculated the score of TCM symptom before the treatment and at several time points after
the treatment (1.5 months after , 3 months after, 6 months after , 9 months after and 12 months after), we
evaluated the curative effect of TCM syndrome at the five time points and observed the level of the cellular
immunity criterias, including CD4、
CD8、
CD4/CD8 and the haemodynamics criterias, including Nitrogen
Monoxidum(NO) and Endothelin(ET). We also assessed the frequency of the cold, the number of times
admission due to acute exacerbation and the annual days of hospital stay. Results: (1)Baseline levels:
There were no statistical significance in gender, smoking, age, course of disease, symptom scores of
TCM, between the two groups at baseline (all P>0.05).（
2）
TCM symptoms: The improvement value in the
treatment group —the total symptom scores of TCM at 1.5 months and 3 months after the treatment
minus the total symptom scores of TCM symptom before the treatment—was higher than that in the
control group (P<0.05). However the improvement value between two groups —the score of TCM
symptom at 6 months，
9 months and 12 months after the treatment minus the score of TCM symptom
before the treatment—was not significantly different（
P>0.05）
,but the level of the reducing scores in the
study group was higher than that in the control group. The symptom of shortness of breath was improved
obviously at 3 months after the treatment in the treatment group (P<0.05), and the symptom of
spontaneous sweating was improved obviously at 1.5 months, 3 months and 6 months in the treatment
group (P<0.05). The symptoms of lack of strength, fatigued spirit, thoracic oppression and shortage of Qi,
were improved at most time points in the treatment group（
P<0.05）
.（
3）the Curative Effect of TCM
Syndrome: The total effective rates in the treatment group at the five time points were
23.81%、
57.14%、
38.10%、
28.57%、
19.05% respectively, and the total effective rates in the control group at
5 different time points were 5.00%、
10.00%、
10.00%、
15.00%、
10.00% respectively. The total effective rate
in the treatment group at 3 months and 6 months after the treatment was higher than that in the control
group（
P<0.05）
.(4) Experimental Data: The levels of CD4、
CD8 and the ratio of CD4/CD8 were not
significantly different（
P>0.05）
; In the treatment group, the level of CD4 and the ratio of CD4/CD8 after the
treatment was higher than that before the treatment（
P<0.05）
, and the level of CD8 was lower than that
before the treatment（
P<0.05）
. In the control group the levels of CD4、
CD8 and the ratio of CD4/CD8 were
not significantly different（
P>0.05）
. The level of NO between the treatment group and the control group had
no significant difference（
P>0.05）
, while the level of ET in two groups was significantly different (P<0.05).
Specifically, in the treatment group the level of NO after the treatment was higher than that before the
treatment（
P<0.05）
, while the level of ET after the treatment was lower than that before the
treatment（
P<0.05）
（
5）
the End-points: The frequency of the cold, the number of times admission due to
164
acute exacerbations and the annual days of hospital stay in the study group were all lower than those in
the control group（
P<0.05）
.Conclusion: The method of “Reinforcing Qi and Activating Blood” can improve
symptoms and clinical curative effect of TCM in the treatment of stable COPD (Qi Deficiency and Blood
Stasis Syndrome). It also can improve their cellular immunological function and vasomotor function. And it
can shorten the frequency of the cold, the number of times admission due to acute exacerbation and the
annual days of hospital stay as well.
A-220
The Study on Levels of Immunoglobulin and Complement in Henoch-schonlein Purpura
Patient with Different Syndrome
Zhang Cheng1, Bai Yusheng 1
1
Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China
Objective: This paper is to study the correlation between HSP patients with different TCM syndrome type
and Immunoglobulin and Complement 3,4 so as to supply the theory basis for objectify of TCM syndrome
type in HSP patients. Methods: established the perfect symptoms observation charts, and selected the
patients in accord with diagnostic criteria and standards criteria, then collected the TCM manifestation of
patients without treatment whom in acute stage. Divided the selected HSP patients into 4 basic TCM
syndrome type; At the same time, test the serum immunoglobulin andcomplement testing. Results:(1)
112 cases of patients with selected HSP, men 53 cases (47.32%), women 59 patients (57.68%), male:
female = 1:1, maximum age 71 years, minimum for 4 years, mean age 23.71 ±15.80 years old, men and
women the disparity between the sexes was not statistically significant.(2). By comparing HSP type
certificate of the patients with average age, the comparison between syndrome types, wind hot injury type
winding minimum average age, and the average age was taken blood type relative to other group is the
largest, each card type average age difference was statistically significant (P>0.05); 3.The group of 112
cases of patients with HSP before treatment, wind hot injury type winding 32 cases (28.57%); Blood hot
injury of 38 cases of type winding (33.93%); Yin deficiency type fire 24 cases (21.43%); Qi not type taken
18 cases (16.07%). 4.Immune globulin IgA level, blood hot injury type winding group higher than other
groups, and the rest of the three groups compared respectively, P<0.05; IgG test results spirit not taken
blood type higher than other three groups, Yin deficiency group compared with fire, P<0.05; IgM test
results, wind hot injury type winding level higher than the other group, with the rest 3 groups were
compared, P<0.05; C3, C4 results Yin deficiency fire type are all lower than those of other groups,
including the C3 level detection, Yin deficiency fire type group was lower than other groups, the blood hot
injury type winding group level is the highest, Yin deficiency fire with blood type heat injury type winding,
wind hot injury type winding comparison group, P<0.05; C4 level detection, Yin deficiency fire type group
level lower than other group, wind hot injury type winding highest group, Yin fire with the other three
groups of comparison, P<0.05. Conclusion:1.the rise of Immunoglobulin IgA has reference value to
judge HSP patients with blood heat syndrome type; while the same on IgM to wind heat syndrome type
and IgG to yin deficiency fire excess syndrome type; 2.The reduce of Serum C3, C4 level tend to yin
deficiency fire excess syndrome type in chronic stage; 3.There were differences on Immunoglobulin
and complement in HSP patients with various types which provides theory basis and reference.
165
A-221
The study on the imbalance of Regulatory T cells/Th17 cells in the different Traditional
Chinese Medicine syndromes of asthma
Yan Lu 1, Zhou Xianmei 1
1Nanjing
University of Traditional Chinese Medicine is affiliated with the Chinese Medicine Hospital of Jiangsu Province,
210029, Nanjing, China
Objective: Bronchial asthma is divided into different syndromes in Traditional Chinese Medicine (TCM).
The imbalance of Regulatory T (Treg) cells/Th17 cells differentiation plays an important role in asthma.
Treg cells and Th17 cells express interleukin (IL-10) and IL-17A, respectively. The current study is to
evaluate Treg cells/Th17 cells differentiation via detecting the IL-10/IL-17A levels in serum, explore
immunifaction of different syndromes in TCM, provide objective proof for TCM syndromes, and further
benefit the clinical therapy. Methods: 15 patients of hot type asthma, 15 patients of deficiency syndrome
of kidney yang, and 15 healthy volunteers were involved. Blood samples were collected from the subjects,
serum was separated and stored at -80 oC. IL-10 and IL-17A levels in the samples were detected with
enzyme-linked immunosorbent assay (ELISA) method. Results: 1. The levels of IL-10 of both hot type
asthma (64.76±8.18) and kidney yang-deficiency (57.62±12.94) groups were decreased compared with
that of volunteers group (104.22±42.07) (P<0.01). 2. Compared with that of volunteers group
(50.26±9.84), the IL-17A level of hot type asthma group (62.05±13.25) was significantly increased
(P<0.01), while, there was no statistical difference between the hot and kidney yang-deficiency types. 3.
The ratios of IL-10 and IL-17A in hot type asthma (1.08±0.20) and kidney yang-deficiency (1.11±0.37)
groups were decreased compared with that of volunteers group (2.10±0.90) (P<0.01). There was no
statistical difference between the hot type asthma (1.08±0.20) and the kidney yang-deficiency asthma.
Conclusion: 1. The hot type asthmas showed a decrease of IL-10, overexpression of IL-17A, and a
reduced ratio of IL-10 and IL-17A, suggesting an imbalance of Treg cells/Th17 cells sourced from a
simultaneous IL-10 decrease and IL-17A increase. 2. The asthmas with kidney yang-deficiency types
displayed a lower level of IL-10, normal level of IL-17A, and a decreased ratio of IL-10 and IL-17A, also
suggesting an imbalance of Treg cells/Th17 cells, but originated from an IL-10 decrease. 3. The results
revealed the asthmas with hot type and kidney yang-deficiency type have some abnormal immunifaction,
but the IL-10/IL-17A or Treg cells/Th17 cells cannot be an objective proof for TCM syndromes.
A-222
To study allergic rhinitis of TCM and IL-4 gene methylation between Uighur nationality
and Han people comparative in Ghulja of Xinjiang
Liu Futai 1, Gao Li 1*
1Traditional
1
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China;*Correspondence should be addressed to Xjmugaoli@sina.com
166
Objective: Allergic rhinitis syndromes in Ghulja area distribution and allergic rhinitis IL-4 comparison of
gene methylation between Uighur nationality and Han people. Allergic rhinitis in the region get TCM
distribution, whether IL-4 gene methylation have difference between Uighur nationality and Han people.
Methods: Using random cluster sampling method and survey of AR patients with TCM syndrome
differentiation to obtain the prevalence of AR and the constituent ratio of TCM. To sure Uighur nationality
and Han people, which have healthy control and patients, extracted from peripheral blood, extracted DNA
in the blood and DNA were modified sulfite for IL-4 MSP amplification, and analyse of the results.
Results: Investigated 2413 people, including 421 patients AR, the prevalence was 17.45%. Uighur
nationality prevalence was 13.76%, which was less than that in Han people (27.43%), P<0.01.It was AR in
446 patients that in line with the number of TCM 242.The highest TCM of syndrome of deficiency of lung qi
(69.01%), followed by deficiency of spleen qi type(13.22%), syndrome of lung heat(9.51%), syndrome of
deficiency of kidney(8.26%). The IL-4 promoter methylation was 51.61% (16/31) in Han AR, unmethylated
was 16.13%(5/31), methylation and unmethy lated was 32.26% (10/31). The complete methylation was
25.81%(8/31) of healthy Han, unmethylation was 6.45%(2/31), methylation and unmethylated was
67.74% (21/31). The IL-4 promoter methylation was 48.38% (15/31) in Uygu AR, unmethylated was
25.81%(8/31), methylation and unmethylation was25.81% (8/31).The complete methylation was
19.35%(6/31) of healthy Uygu ,unmethylation was 38.71% (12/31), methylation and unmethylated was
41.94% (13/31).The AR methylation compared with the control groups of Han people (P<0.05), The AR
methylation compared with the control groups of Uighur nationality (P>0.05).It was no difference of AR
between Han and Uighur nationality (P>0.05),There have significant differences with healthy control of
methylation complete between Han people and Uighur nationality(P<0.01). Conclusion: The prevalence
of AR in Uygu nationality, which was less than that in Han people, deficiency syndromes in lung type than
the other three types. Climate and environment that have a certain influence on the AR. There have some
influence on the incidence between Uighur nationality and Han people living habits and environment. It is
no difference between the two nations AR positive, but in Han people have a difference in IL-4 gene
methylation. It was a feasible approaches as the study of allergic rhinitis that IL-4 gene methylation. The
methulated and unmethylated of Uighur healthy controls which was less than Han. The IL-4 methylation of
Han people might tend to expression which susceptible to allergic rhinitis.
A-223
To study the relative of Kidney asthenia blood stasis type deafness and mtDNA 12s rRNA
gene mutation in deafness of Uighur ethnic in Xinjiang region of China
Sun Xuefen1, Li Yanhua1*
1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi
830011, Xinjiang Uighur Autonomous Region, China;
*Correspondence should be addressed to liyanhua1953@126.com
Objective: To study the relation of Kidney asthenia blood stasis type deafness and mtDNA 12srRNA
gene mutation, the normal people and sensorineural hearing loss in patients in the Uighur nationality, and
compare the differences between the two groups; To study the relevance between mutation of deafness
with kidney deficiency and blood stasis with mtDNA12S rRNA. Methods: 137 deaf patients and other 93
normal person had been gathered, 137 cases which tinnitus and deafness with differentiation of
symptoms and signs for classification of syndrome were divided into kidney asthenia blood stasis (105
167
cases) and non-blood stasis Kidney and Blood(32 cases) to obtain the blood from the 230 samples
people, the mtDNA12srRNA were examined by polymerase chain reaction(PCR), and statistics analysis
the relation between Kidney asthenia blood stasis type deafness and mtDNA12srRNA. Result: Many
types the mutation had been detected of mtDNA12srRNA and the mutation ratio of A1555G was
4/105(3.8%) higher in patients with hearing loss than the average. The disease-causing mutations
T961cn、A827G、
nt955ccccnt960 were detected. All of them were not detected in the normal person.
Many types polymorphism the mutation had been detected of mtDNA12srRNA: G709A、A750G、A1438G
and they bear out the mtDNA mutation polymorphic. We also found a new case of mutant forms:
nt950cccccnt956, the pot nt950cccccnt956 may be the new disease-causing mutations type. The
differences between Hearing loss group and by mutations in the control group were statistically analyzed,
The results showed that the difference was statistically significant,(P<0.05). There were statistically
significant differences between Kidney asthenia blood stasis type and mtDNA12S rRNA gene mutation ,
There was significant difference (P<0.05), that is Kidney asthenia blood stasis type in Blood Uighur has
the relationship with mtDNA12S rRNA gene mutations. Conclusion: There were mutually relation
between the type of kidney asthenia blood stasis and mtDNA 12srRNA gene mutation in Uighur patients
with hearing impairment in Xinjiang. Kidney asthenia blood stasis type might be the reason of mtDNA
12srRNA gene mutation in the Xinjiang region, and there were certain ethnic and geographical
characteristics. That carry mutations in the gene for deaf individuals with disease susceptibility, so
scientific intervention will help reduce or delay the hearing loss diseases.
A-224
Oncothermia combination with Traditional Chinese Medicine: network approach
Hegyi1 Szasz2 A, G. Andocs3 G. Szasz1,4,5
1Department
of Complementary Medicine, Medical School, University of Pecs, Pecs, Hungary; 2Department of Biotechnics,
Faculty of Engineering, St. Istvan University, Budapest, Hungary, 3Department of Pharmacology and Toxicology, Faculty of
Veterinary Science, St Istvan University, Budapest, Hungary; 4OncoTherm Kft. Paty, Hungary; 5OncoTherm GmbH, Troisdorf,
Germany;
Background: Acupuncture and their connective pathways the meridians are ancient Chinese knowledge
but it is not understood yet in details. Request of the stable homeostasis of the complex organisms is
demanding interdisciplinary approach and new paradigm for the topic. The detecting and reconstructing
the deviation from the normal balance of the homeostasis is the basic principle of TCM. The Chinese
herbs, the physical (mechanical or electromagnetic acupuncture, acupressure) and mixed forms of
heating and diffusion-therapies (moxa therapies) approaches are pointing these problems, and solving it
with ancient methods. Oncothermia method (OTM) uses also the deviations from the normal homeostasis
for selecting the tumor cells and on this basis ignite natural processes to eliminate them from the system,
reestablishing the communication harmony between the cells. This technique is well proven from the
laboratory level to the clinical applications. Our aim is to synergize the TCM with OTM on the common
basis of equilibrium demand; and use the recognition of the deviations from the complex harmony of the
organism or its part for selection to act properly. Method: An extended network approach was presented
in the topic, and we would like to continue our research on this basis. The in silico studies will have their
roots from the network analysis together with the modern fluctuation theory for complex living organisms
(fractal-physiology) was developed in the last decades to study this complexity: like self-organization ,
168
fractal physiology, and the bioscaling. Oncothermia widely using these new scientific results; as well as
the resonance phenomenon is studied and used in the light of a new theory, and special vector-potential
theory helps to complete the method. The problems of the thermal limit in the deep-seated tissues is
theoretically and experimentally solved, so it has no any barrier for the wide investigations in synergy
experiments. TCM involves electro-acupuncture and laser acupuncture, which are similar in their
electromagnetic (conductive) approach to oncothermia effects. The in vivo studies will be performed using
a BalbC/nu/nu mice xenografts; using specialized experimental setup (EHY110, Oncotherm, Germany).
The effects will be studied histomorphologically (HM) and immunhistochemically (IHCH) by various
antibodies with state-of-art high tech digital microscopy system (MiraxWiev, 3D Histech). Conclusion:
Recognition of the distortions in the healthy tissue have some common principles and possibilities in TCM
and OTM. The synergy of the ancient knowledge and the high-tech state-of-art of the medical knowledge
could be established with this research.
A-225
Airway inflammation and hypothalamic-pituitary-adrenal axis function in kidney-yang
deficiency type of Chinese medicine in asthma and chronic obstructive pulmonary
disease
Du Yijie1, Li Bei 1, Cao Yuxue 1, Liu Baojun 1, Wu Jinfeng1, Zhang Hongying 1, Jin Hualiang 1, Dong
Jingcheng 1*
1Lab of Integrative Medicine for Lung,
Inflammation and Cancers, Huashan Hospital, Fudan University, Shanghai 200040, China;
Correspondence to: Jing-cheng Dong, M.D., Ph.D, Professor of Lab of Integrative Medicine for Lung, Inflammation and Cancers,
Huashan Hospital, Fudan University, Shanghai 200040, China (Tel: 86-21-52888301. Fax: 86-21-52888265. Email:
jcdong2004@gmail.com)
Background: Bronchial asthma (BA) and chronic obstructive pulmonary disease (COPD) are both
inflammatory airway diseases with different characteristics, but they are often involved with kidney-yang
deficiency type of Chinese medicine(KYD). Our study aimed to evaluate changes of inflammatory airway
features and the hypothalamic-pituitary-adrenal (HPA) axis function in KYD-BA compared with
KYD-COPD. Methods: Thirty-six adults with KYD-BA, 37 with KYD-COPD, 30 with non-KYD-BA and 26
with non-KYD-COPD were recruited in our study. Forty-one healthy individuals were enrolled as controls.
Sputum was induced and blood samples were collected for measurement of cytokines and other
inflammatory factors. The 24-hour collection of urine was performed and salivary samples for the diurnal
rhythm profiles were obtained for assessment of the HPA axis activity. Results: Serum and sputum IgE,
and sputum IL-2 in the KYD-BA group were higher than in the healthy control group (P<0.05). Serum IL-2
and IL-6, and sputum IL-6 and TNF-α in the KYD-COPD group were higher than in the healthy control
group (P<0.05). Sputum eosinophil cationic protein(ECP) concentrations were significantly higher in
KYD-BA group compared to other groups (P<0.001). Serum IL-6, serum TNF-α, and sputum TNF-α in
KYD-COPD group were higher than in KYD-BA group (P<0.05). The 24-hour urinary
17-hydroxycorticosteroids/17-ketosteroids concentrations were lower in KYD-BA and KYD-COPD groups
than in healthy control group (P<0.05). The peak level and baseline level of the diurnal rhythm were also
significantly lower in KYD-BA and KYD-COPD groups compared to other groups (P<0.001).
Conclusions: In this study of KYD-BA and KYD-COPD patients, we found that the features of
inflammation in KYD-BA and KYD-COPD groups were enhanced. The HPA axis functions in these groups
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are disturbed or impaired more severely, which may be involved in the pathogenesis of KYD.
A-226
Baicalin restores corticosteroid sensitivity in cigarette smoke induced inflammation by
modulating HDAC2 in vivo and in vitro
Li Lulu 1, Wu Jinfeng 1, Liu Baojun 1, Sun Jing 1, Gong Weiyi 1, Lv Yubao1, Zhang Hongying 1, Luo Qingli 1, Wu
Xiao 1, Dong Jingcheng 1*
1Department
of Integrative Medicine, Huashan Hospital, Fudan University, 12 Middle Urumqi Road, Shanghai 200040, China;
*Corresponding author: Tel.: +86 21 52888301, Fax: +86 21 52888265, E-mail address：
jcdong2004@hotmail.com
Chronic obstructive pulmonary disease (COPD) is a common chronic inflammatory disease of the lungs with
little or no response to corticosteroids and a high level of oxidative stress induced by cigarette smoke. In this
study, we investigated the mechanism underlying the restoration of corticosteroid sensitivity in cigarette smoke
induced inflammation by baicalin, a flavonoid compound isolated from the root of Scutellaria baicalensis
Georgi, in vivo and in vitro. In vivo, mice were exposed to smoke of 15 cigarettes for 1 h/day, 6 days/week for 3
months and dosed with baicalin (25, 50 and 100 mg/kg) or dexamethasone (1 mg/kg). In vitro, A549 cells were
incubated with baicalin (10, 50 and 100 μM) or dexamethasone (10-12, 10-10, 10-8 and 10-6 M) followed by
treatments with CSE (2.5, 5%), or TNF-α (10 ng/ml), or trichostatin A (100 ng/ml). Here we reported the
discovery that baicalin significantly protected pulmonary function and attenuated CS-induced inflammatory
response by decreasing inflammatory cells and production of TNF-α, IL-8 and MMP-9, which was poorly
achieved by dexamethasone. Baicalin also showed efficacy on enhancing HDAC2 activity and protein
expression, however, it did not affect HDAC2 mRNA. Further studies revealed that baicalin inhibited HDAC2
phosphorylation, suggesting it may directly affect the protein structure and effect by modification at
post-translational level. Together these results suggest that baicalin plays a critical role in modulating HDAC2,
leading to restoration of corticosteroid sensitivity, finally attenuating CS-induced respiratory inflammation.
A-227
Establishment and comparison of combining disease and syndrome model of asthma
with ‘kidney Yang deficiency’ or ‘abnormal Savda’
Li Bei 1, Luo Qingli 1, Mammat Nurahmat 2, Du Yijie 1, Jin Hualiang 1, Wu Xiao 1, Lv Yubao 1, Sun Jing 1,
Dong Jingcheng 1*
1
Department of Integrative Medicine, Huashan Hospital, Fudan University, Shanghai, 200040 China; 2 Xinjiang Uighur Medical
Training College, Wada, Xinjiang, 848000 China; *Corresponding author: Tel/fax: +86 21 52888301. E-mail address:
jcdong2004@126.com (J. Dong).
Objective: Firstly, our study aims to establish and evaluate rat models of kidney Yang deficiency
syndrome (KYDS) and abnormal Savda syndrome (ASS). General state, body weight, food and water
intake, behaviors, function of hypothalamic-pituitary-target organ axes (HPTOA) and
sympathetic/parasympathetic (S/P) nerve system were observed and compared between the two rat
models. Secondly, the study aims to establish and evaluate rat models of combining disease and
syndrome model of asthma with KYDS or ASS. General state, body weight, food and water intake,
behaviors, function of HPTOA and S/P nerve system, airway hyperresponsiveness (AHR), airway
170
inflammation, and airway remodeling were recorded and compared between the two rat models.
Methods: The‘KYDS’model was induced by high dose of exogenous corticostrone by multi-point
subcutaneous injection; the‘ASS’model was induced by multiple factors including reared in a dry and cold
environment, given dry and cold food, experienced chronic intermittent plantar electric shock, forced
swimming and fixed brake; Then, the above mentioned rat models were respectively sensitized and
challenged by ovalbumin (OVA) and models combining disease and syndrome of asthma
with‘KYDS’or‘ASS’were established. Sixty male SD rats were randomly divided into six groups: normal
control group (NC), asthma control group (AC), kidney Yang deficiency syndrome group (KYDS),
abnormal Savda syndrome group (ASS), kidney Yang deficiency syndrome –asthma group (KYDSA), and
abnormal Savda syndrome-asthma group (ASSA). General state, body weight, food and water intake
were recorded everyday; the open field test (OFT) and sucrose preference test (SPT) were used to
assess changes of behavioral indicators; the weight of thymus, spleen, adrenal, thyroid and testicular
were measured and organ index of those glands were calculated; Enzyme-linked immunosorbent assay
(ELISA) method was used to observe the level of corticostrone (CORT), adrenocorticotropic hormone
(ACTH), 3,5, 3′-triiodothyronine (T3), thyroxine (T4), thyroid-stimulating hormone (TSH), estradiol (E2),
testosterone (T), luteinizing hormone (LH), follicle stimulating hormone (FSH), cyclic adenosine
monophosphate (c AMP) and cyclic guanosine monophosphate (c GMP) in plasma in order to assess the
function of HPTOA and S/P nerve system; Buxco's new FinePointeTM RC system was used to detect
airway resistance (RI) and AHR in response to methacholine (Mch); Bio-Plex suspension array
technology was applied to detect the level of cytokines such like interleukin (IL)-2, IL-5, IL-6, IL-10, IL-13,
granulocyte-macrophage colony stimulating factor (GM-CSF) and interferon-γ (IFN-γ) in bronchoalveolar
lavage fluid (BALF) and serum; Hematoxylin-eosin (H&E) staining was used to evaluate perivascular
eosinophilia, peribronchiolar eosinophilia, epithelial damage and oedema in the lungs of rats; Periodic
Acid-Schiff (PAS) staining was utilized to assess mucus secretion; Masson's staining was applied to
assay the amount of collagen deposition. Results: Compared to the NC group, body weight of the other
five groups were significantly reduced after modeling (P<0.01), and the ASS group was the most obvious
group in body weight loss. Moreover, body weight was slightly restored in the groups of combining
disease and syndrome during the process of allergy exposure (P<0.05), but there were still significant
differences with the NC group (P<0.05). The weight loss of ASSA group was more serious than that of
KYDSA group (P<0.05); Food and water intake were markedly increased in the ASS with or without
allergy exposure groups, but decreased in the KYDS with or without allergy exposure groups compared to
the NC group (P<0.01). Moreover, food and water intake in the AC group were less than the NC group
(P<0.01); The results of OFT demonstrated that the counts of total cross, horizontal activity and vertical
activity were markedly diminished in the KYDS, KYDSA, ASS and ASSA groups in contrast to the NC
group (P<0.01). However, the activities included fecal boli in OFT were significantly increased in the AC
group (P<0.01); The results from SPT showed that preference for sucrose was sharply declined in the
groups of KYDS with or without allergy exposure and ASS with or without allergy exposure (P<0.01), in
addition, the ASS group was the most obvious group; The organ index showed that OVA-exposed
induced thymus and spleen hyperplasia implied immune-activated (P<0.01), and thymus in the rats of
KYDS and ASS groups were atrophy indicated immune-suppressed (P<0.05, P<0.01); the KYDS with or
without allergy exposure group manifested as adrenal and thyroid atrophy (P<0.05), but testicular
hyperplasia (P<0.05). Above three glands were hyperplasia in the ASS with or without allergy exposure
group (P<0.05). The adrenal of AC group was atrophy (P<0.05), but there was no significant difference in
thyroid and testicular; ELISA results indicated that the levels of CORT, T4, TSH, LH, FSH notably
171
increased in plasma in the groups of KYDS with or without allergy exposure group (P<0.01), but the level
of ACTH significantly decreased (P<0.05) compared to the NC group, which demonstrated those groups
had the dysfunction of HPTOA. In contrast to the NC group, the levels of hormones such as CORT,
ACTH, T4, TSH, LH, FSH were sharply increased in the ASS with or without allergy exposure (P<0.01),
which indicated activating HPTOA. Although the level of CORT dropped down and ACTH dropped up in
the AC group, there was no significant difference with the NC group. The level of c AMP and the ratio
between c AMP and c GMP remarkably reduced in the other five groups compared with the NC group
(P<0.01), which indicated that there was the dysfunction of S/P nerve system in those groups; The results
of invasive pulmonary function test illustrated that OVA-challenged rats developed AHR compared to
those without allergy exposure, typically reflected by high RI in response to increasing concentrations of
Mch (P<0.05，
P<0.01). RI was higher in the KYDSA group than that in the AC group (P<0.05), but was
lower than that in the ASSA group at the Mch concentrations of 12.5mg/ml and 25mg/ml. Moreover, the
change of RI from baseline was significantly increased in the ASSA group compared to the AC group at
every concentrations of Mch (P<0.05); Bio-Plex method showed that OVA-exposed sharply increased the
levels of Th2 inflammatory cytokines like IL-6, IL-13 in BALF and serum (P<0.01), but Th1 cytokines like
IL-2, IL-10 was markedly decreased (P<0.01) compared to those without allergy exposure, which
illustrated that OVA-exposed induced the imbalance of helper T cells 1(Th1)/Th2. Though linear
correlation analysis, changes of some cytokines in serum were consistent with those in BALF; Lung
histopathological studies showed that inflammatory cells especially eosinophils and lymphocytes
infiltration in the peribronchial and perivascular areas (H&E staining), mucus overproduction and goblet
cell hyperplasia (PAS staining) and emerge of collagen deposition (Masson staining) were observed in
the OVA-exposed groups (P<0.01). Moreover, the degree of inflammatory cells infiltration in the
peribronchial and perivascular areas (P<0.05) and emerge of collagen deposition (P<0.05) were more
serious in the KYDSA group compared with the AC group. The scores of inflammatory cells infiltration
(P<0.05) and collagen deposition were higher in the ASSA group compared to the AC group.
Conclusion: On the one hand, usage of the high dose of corticosterone or multiple factors could
successfully establish the KYDS or ASS rat model, and the two models had similar changes in biological
characterization, abnormal behaviors, dysfunction of HPTOA and S/P nerve system, but varied in
different degree. On the other hand, based on above rat models and then combined with ovalbumin
exposure could successfully set up models of combining disease and syndrome of asthma with KYDS or
ASS, moreover, those models had either the characteristic of pathology of asthma such as AHR, airway
inflammation, airway remodeling, which were more serious than allergy exposure alone, or the syndrome
performance of Kidney-Yang deficiency in traditional Chinese medicine (TCM) and Abnormal Savda in
traditional Uighur medicine (TUM), which indicated the feasibility and reliability of those models.
A-228
Icariin attenuates LPS-induced acute inflammatory responses: Involvement of PI3K/Akt
and NF-κB signaling pathway
Xu Changqing 1, Liu Baojun 1, Wu Jinfeng 1, Xu Yanchun 2, Duan Xiaohong1, Cao Yuxue 1, Dong
Jingcheng 1*
1
Lab of Lung, Inflammation and Cancers, Huashan Hospital, Fudan University, Shanghai, 200040, China;
172
2
Department of
Physiology and Pharmacology, West Virginia University, WV, 26506, USA; *Corresponding author: Tel.: +86 21 52888301, Fax:
+86 21 52888265, E-mail address：
jcdong2004@hotmail.com
This study aimed to investigate the mechanism underlying the attenuation of LPS-induced lung
inflammation by icariin in vivo and in vitro. The anti-inflammatory effects of icariin on LPS-induced acute
inflammatory and the molecular mechanism were investigated. Pretreatment with icarrin (20 mg/kg) could
attenuate acute lung inflammation by inhibiting mRNA expressions of tumor necrosis factor alpha
(TNF-α), interleukin-6 (IL-6), metalloproteinase cycloxygenase-2 (COX-2), and inducible nitric oxide
synthase (iNOS) in the lung of LPS-treated mice. In addition, icariin suppressed the secretion of TNF-α,
prostaglandin E2(PGE2) and nitric oxide (NO) as well as NF-κB p65 activation. Furthermore, decreased
myeloperoxidase(MPO) activity was observed in the lung tissue and LPS-induced cytotoxicity in the RAW
264.7 macrophages cells was also markedly attenuated by icariin. Western blotting analysis and confocal
microscopy showed that icariin pretreatment reduced the nucleus transportation and constant level of
NF-κB p65 in the RAW 264.7 macrophage cells. However, the protective effects of icariin were reversed
by a PI3K/Akt inhibitor(wortmannin). Our in vitro and in vivo results suggested that activation of the
PI3K/Akt pathway and the inhibition of NF-κB were involved in the protective effects of icariin on
LPS-induced acute inflammatory responses.
A-229
Icariin inhibits corticosterone-induced apoptosis in hypothalamic neurons via the
PI3-K/Akt signaling pathway
Zhang Hongying 1, Liu Baojun 1, Wu Jinfeng 1, Xu Changqing 1, Tao Jiang 2, Duan Xiaohong 1, Cao Yuxue
1, Dong Jingcheng 1*
1Laboratory
of Lung, Inflammation and Cancers, Huashan Hospital, Fudan University, 200040, Shanghai, China; 2Department of
General Dentistry, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of
Stomatology, 200011, Shanghai, China; *To whom correspondence should be addressed (Full postal address :Laboratory of
Lung, Inflammation and Cancers, Huashan Hospital, Fudan University, Shanghai, China 200040.Telephone +86-21-52888301;
Fax +86-21-52888265; E-mail: jcdong2004@hotmail.com)
Excessive corticosterone is known to induce neuronal damage in many regions of the brain，
particularly
the hippocampus, which is the mainly area implicated in depression. However, little research has been
done on alterations to hypothalamic neurons in depression and the cellular and molecular basis for the
changes. In the present study, we aim to determine whether corticosterone causes apoptosis in primary
cultured hypothalamic neurons and to investigate the protective effects of icariin, an active natural
ingredient from the Chinese plant, Epimedium sagittatum maxim. Our study shows that exposure of
hypothalamic neurons to corticosterone causes a significant loss in viability, a significant decrease in
mitochondrial membrane potential (MMP), an increase in caspase-3 activity ,an elevation in intracellular
reactive oxygen species (ROS) elevation and decreased superoxide dismutase (SOD) activity . However,
pretreatment of the cells with icariin prior to corticosterone exposure was founded noticeably suppress
these corticosterone -induced events. noticeably. Furthermore, icariin can prevent corticosterone-induced
cell death via activation of PI3/Akt pathway. In conclusion, icariin can prevent corticosterone-induced
hypothalamic cell apoptosis via activation of the PI3/Akt pathway.
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A-230
Pharmacokinetics of forsythoside after intravenous administration in beagle dogs
Shi Rong 1, Xuan Zhenyu 2, Ma Yueming 1, L.Yingying 1, L.Hongguo 3, S.Tianjiang 3
1Laboratory
of Pharmacokinetics, Shanghai University of Traditional Chinese Medicine, Shanghai, China; 2Shanghai Youseen
New Medicine Exploitation Co, Ltd, China Shanghai, China; 3Yangtze River Pharmacy Group, Taizhou, China
A high-performance liquid chromatography with ultraviolet detection method was established and
validated for quantification of forsythoside concentrations in dog plasma. Following a single-step protein
precipitation with perchloric acid, the forsythoside and internal standard were separated on a
reversed-phase C18 column with water-glacial acetic acid-methanol as mobile phase at a flow rate of 1
mL/min with ultraviolet detection at 326 and 278 nm for forsythoside and IS, respectively. The calibration
curve for forsythoside was linear over a range of 0.052-13.33 ug/ml, with correlation coefficient of 0.999.
The within- and between-batch precisions of analysis were <8 % and accuracy was 95-107%. After
intravenous administration of forsythoside at the doses of 5, 10, and 20 mg/kg, the Cmax values for
forsythoside were estimated to be of 12.33, 22.90 and 54.45 ug/mL, respectively. The AUC increased
with the increasing of doses, and the mean AUC0-t values were 5.69, 11.80, and 18.66 mg·h/L,
respectively. Forsythoside was eliminated quickly and the mean T1/2 values at doses of 5, 10, and 20
mg/kg were 1.36, 1.49, and 0.71h, respectively. The pharmacokinetics of forsythoside in beagle dogs
complied with linear kinetic course in the dose range.
A-231
Research on the correlation between expression of foxp3 protein and airway
inflammation and the effects of Icariin in a mouse model of asthma
Jin Hualiang 1, Li Bei1, Luo Qingli 1
1Lab
of Integrative Medicine for Lung，
Inflammation and Cancer，
Huashan Hospital，
Fudan University，
Shanghai 200040, China
Objective: To investigate the role of FOXP3 protein expression within the CD4+CD25+Foxp3+Treg cells
in the pathogenesis of asthm in a mouse model, and explore the effects of icarrin on the airway
inflammation and expression of FOXP3. Methods: 32 Balb/c mice were randomly divided into 4 groups,
i.e., the normal control group, the model group, the dexamethasone group, the icariin group, with 8 mice in
each group. Asthma model was established by ovalbumin sensitization and aerosol challenge in all
groups except the control group. The mice of each treatment group were administrated with
dexamethasone and icariin respectively by gavage, while mice in the normal control group were
administrated with physiological saline. Within 24 houres after the last ovalbumin challenge, airway
reactivity was measured by the invasive measurement system. Changs in airway histopathology were
observed. Polychromatic flow cytometry was applied to delineate the CD4+CD25+Foxp3+Treg cells in
spleen. The level of FOXP3 protein expression in lung tissue was assessed by Westem blot. IL-10 in
BALF and serum were measured by ELISA. Results: Compared with the control group, the airway
hyperresponsiveness and airway inflammation was significantly aggravated in model group (P < 0.05).
The percentage of Treg cells was similar between the control and model groups (P < 0.05). The average
expression of FOXP3 protein within Treg cells in spleen and lung tissue was significantly decreased (P <
0.05). In BALF and serum of the asthma group, the level of IL-10 was signifivantly lower (P < 0.05).
174
Dexamethasone treatment significantly attenuated airway hyperresponsiveness and inflammation, and
increased the average expression of FOXP3 protein within Treg cells in spleen and total FOXP3 protein in
lung tissues, and the IL-10 levels in BALF and serum were increased ; Icariin also significantly attenuated
airway hyperresponsiveness and inflammation, and increased the expression of total FOXP3 protein in
lung tissues and IL-10 levels in serum, and had a tendency to increase the average FOXP3 expression
within Treg cells in spleen and IL-10 level in BALF. Conclusion: In a mouse model of asthma, the
expression of FOXP3 protein is decreased within their CD4+CD25+Foxp3+Treg. Our findings also
suggests that icariin may increase the FOXP3 expression in lung tissue and the level of IL-10, which may
contribute to its anti-inflammatory effects.
A-232
Some Chinese Medicine Components Attenuating Oxidative Stress and Airway
Remodeling in COPD Rat via Keap1-Nrf2-ARE Signaling Pathway
Lv Yubao1, Li Lulu 1, Sun Jing 1, Gong Weiyi 1, Jin Hualiang 1, Luo Qingli 1, Du Yijie 1, Li Bei 1, Wang Genfa
1, Dong Jingcheng 1*
1Department
of Integrative Medicine, Huashan Hospital, Fudan University, 12 Middle Urumqi Road, Shanghai 200040, China
*Corresponding author: Tel.: +86 21 52888301, Fax: +86 21 52888265, E-mail address：
jcdong2004@hotmail.com
Objective: Investigating how Icariin, Astragaloside ,Catalpol, Baicalin and their compounds to reduce
oxidative stress and airway remodeling in COPD rat. Methods: (1) SD rats were grouped and modeled as
same as part 1. (2).To observe the level of Nrf2 expression in the lung with immunohistoc-hemistry. (3)
Taking the right lung tissue to extract mRNA and observing the expression of Keap1, Nrf2, CAT, GCLC,
HO-1, SOD, NQO1 mRNA with Real-time-PCR. (4) Evaluation of Nrf2, GCLC, CAT, HO-1, NQO1 and
Keap1 protein.exp-ression in lung tissue by Western blotting. Results: (1) Compared with the control
group, the immunohistochemical positive expression of Nrf2 in model group was significantly higher (P
<0.05), while Nrf2 expression in the Dexamethasone group was not statistically significant compared with
the model group (P> 0.05). Compared with the model group, the levels of Nrf2 expresssion in the NAC
group, the Icariin group, the Astragaloside group, the Catalpol group, the Baicalin group and the
compound groups rised with the doses increased. the difference was significant (P<0.05). (2)
Real-Time-PCR showed that the expression of Nrf2 mRNA in the model group was significantly higher
than that of the normal control group (P<0.05); the expression levels of CAT, GCLC, HO-1, SOD, and
NQO1 mRNA tended to increase, but there is no significant difference (P>0.05); Keap1 mRNA expression
tended to decrease, but it was not statistically significant (P>0.05). Compared with the model group, the
expression of Keap1, Nrf2, CAT, GCLC, HO-1, SOD and NQO1 mRNA in the Dexamethasone group was
not statistically significant (P>0.05). The expression levels of Nrf2,CAT,GCLC, HO-1,SOD, NQO1 mRNA
in the NAC group , the Icariin group, the Astragaloside group, the Catalpol group, the Baicalin group and
the compound groups were enhanced with the dose of drugs increased, and at the highest dose, the
differences were significant (P<0.05). However, the keap1 mRNA expression went down with the
increasing of the dose of drugs, at the highest dose, the expression level was significantly lower than that
of the model group, the difference was significant (P<0.05). (3) Nrf2 protein expression was significantly
higher in the model group than the normal control group(P<0.5), the expression levels of CAT, GCLC,
HO-1 and NQO1 protein tended to increase, but there was no significant difference (P>0.05), Keap1
protein expression decreased slighly, but it was not statistically significant (P>0.05). The tendence of
175
Keap1, Nrf2, CAT, GCLC, HO-1 and NQO1 protein expression in the Dexamethasone group was
consistent with the model group, it was not statistically significant (P>0.05). Compared with the model
group, the expression levels of Nrf2, CAT, GCLC, HO-1, NQO1 protein in the NAC group, the Icariin
group, the Astragaloside group, the Catalpol group, the Baicalin group and the compound groups were
increased with the dose of drug raised, the difference was statistically significant (P<0.05); Keap1 protein
expression decreased with the dose of drugs increased, at the highest dose, the difference was
statistically significant (P<0.05). Conclusion: 1) NAC, Icariin, Astragaloside, Catalpol, Baicalin and their
compound formulas could promote Nrf2 protein expression and nuclear translocation in the lung of COPD
rat induced by cigarette smoke; 2) NAC, Icariin, Astragaloside, Catalpol, Baicalin and their compound
formulas increased Nrf2 protein and Nrf2 mRNA expression, strengthening with the increasing of the
dose; 3) NAC, Icariin, Astragaloside, Catalpol, Baicalin and their compound formulas could down-regulate
Keap1 protein and mRNA expression in the lung of COPD rat induced by cigarette smoke. 4) NAC, Icariin,
Astragaloside, Catalpol, Baicalin and their compound formulas could activate Nrf2 downstream pathway,
and increase antioxidant enzymes and detoxifying enzymes in‖phase expression in protein and mRNA
level, strengthening with the dose rised; 5) Icariin, Astragaloside, catalpol, baicalin and their compound
formulas could alleviate oxidative stress damage and airway remodeling in COPD rats induced by
cigarette smoke, which might be associated with the activation of Keap1- Nrf2- ARE signaling pathway.
A-233
The Effects of Icariin, Baicalin on Keap1-Nrf2-ARE Signaling Pathway and Beas-2B Cells
Induced by Cigarette Smoke Extract (CSE)
Lv Yubao 1, Li Lulu 1, Sun Jing 1, Gong Weiyi 1, Jin Hualiang 1, Luo Qingli 1, Du Yijie 1, Li Bei 1, Wang Genfa
1, Dong Jingcheng 1*
1Department
of Integrative Medicine, Huashan Hospital, Fudan University, 12 Middle Urumqi Road, Shanghai 200040, China
*Corresponding author: Tel.: +86 21 52888301, Fax: +86 21 52888265, E-mail address：
jcdong2004@hotmail.com
Objective: This study was to investigate the effect of Icariin & Baicalin on oxidative stress and
Keap1-Nrf2-ARE signaling pathway in human bronchial epithelial cells (Beas-2B) induced by CSE, and
explore their active mechanism. Methods: (1) Cultured bronchial epithelial cells were divided into eight
groups: Group A: normal control group; Group B: model group stimulated by tobacco extract (CSE);
Group C: pretreating low-dose (20mmol/L) Icariin for 24h then stimulating CSE for 4h; Group D:
pretreating middle-dose (40mmol/L) Icariin for 24h then stimulating CSE for 4h; Group E: pretreating
high-dose (80mmol/L) Icariin for 24h then stimulating CSE for 4h; Group F: pretreating low-dose
(20mol/L) Baicalin for 24h then stimulating CSE for 4h; Group G: pretreating middle-dose (40mol/L)
Baicalin for 24h then stimulating CSE for 4h; Group H: pretreating high-dose (80mol/L) Baicalin for 24h
then stimulating CSE for 4h. (2) Cell morphology was observed under an inverted microscope. (3) Cell
viability was assayed by CCK-8. (4) The Concentrations of ROS, IL-8, IL-10, GSH, CAT, SOD and HO-1 in
the supernatant of Beas-2B cell were measured by biochemical methods. (5) The expression levels of
Nrf2、
Keap1 mRNA in Beas-2B were detected by Real -time PCR. (6) The expression levels of Nrf2,
Keap1, NQO1, GCS, HO-1 protein were detected by Western blotting. Results: (1) 10% of CSE induced
oxidative stress injury and death of Beas-2B cell,cell viability considerably decreased compared with the
normal group, the concentrations of ROS, IL-8 in the supernatant were considerably increased (P<0.05),
the concentrations of IL-10, GSH, CAT, SOD, HO-1 levels went down significantly (P<0.05). (2) Compared
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with the model group, Icariin & Baicalin significantly reduced CSE-induced Beas-2b cell death and
inhibited the reduction of cell viability. However, after pretreating Icariin & Baicalin, the concentrations of
ROS & IL-8 in the supernatant were on the decrease with the dose increased, while the concentrations of
IL-10, GSH, CAT, SOD, and HO-1 were elevated with the dose rising, when the concentrations of Icariin &
Baicalin were greater than 40mol/L. they all showed a significant statistical difference at medium or high
dose(P<0.05). (3) Compared with the normal group, the expression of Nrf2 mRNA increased in the
Beas-2B cell induced by 10% CSE, while Keap1 mRNA expression decreased, that is a statistical
significance (P<0.05). Compared with the model group, Icariin & Baicalin significantly increased the
expression of Nrf2 mRNA in the Beas-2B cell induced by CSE and reduced Keap1 mRNA expression in a
concentration-dependent manner. (4) Compared with normal group, the expression of Nrf2 mRNA
increased in the Beas-2B cell induced by 10% CSE, while Keap1 mRNA expression decreased, that is a
statistical significance (P<0.05).Compared with the model group, Icariin & Baicalin obviously increased
the expression of Nrf2 mRNA in the Beas-2B cell induced by CSE and reduced Keap1 mRNA expression
in a concentration-dependent manner. (5) Compared with the normal group, the expression of Nrf2, GCS,
NQO1, HO-1 proteins increased in the Beas-2B cell induced by 10% CSE, while Keap1 protein
expression substantially decreased (P<0.05). Compared with the model group, Icariin & Baicalin
considerably increased the expression of Nrf2, GCS, NQO1, HO-1 protein in the Beas-2B cell induced by
CSE and reduced Keap1 protein expression in a concentration-dependent manner. Conclusion: (1) 10%
CSE could induceBeas-2B cells oxidative stress damage and death. (2) Icariin & Baicalin can clearly
inhibit CSE-induced Beas-2B cell death and improve the cell viability. (3) Icariin & Baicalin inhibited the
generation of endogenous oxide, with increasing of GSH, HO-1, SOD and IL-10. (4) Icariin & Baicalin
activated Nrf2 downstream pathway and increased the antioxidant enzymes and‖phase detoxifying
enzymes in protein and mRNA level in a concentration-dependent manner. (5) Icariin & Baicalin reduced
oxidative stress injury in the CSE induced Beas-2B cell, which might associate with activation of the
Keap1-Nrf2-ARE signaling pathway.
A-234
The Effects of some Chinese Medicine Components on Oxidative Stress and Airway
Remodeling of COPD Rats
Lv Yubao 1, Li Lulu 1, Sun Jing 1, Gong Weiyi 1, Jin Hualiang 1, Luo Qingli 1, Du Yijie 1, Li Bei 1, Wang Genfa
1, Dong Jingcheng 1*
1Department
of Integrative Medicine, Huashan Hospital, Fudan University, 12 Middle Urumqi Road, Shanghai 200040, China
*Corresponding author: Tel.: +86 21 52888301, Fax: +86 21 52888265, E-mail address：
jcdong2004@hotmail.com
Objective: By studying the effects of Icariin, Astragaloside, Catalpol, Baicalin and their compounds for SD
rats with chronic obstructive pulmonary disease, and the changes of lung histopathology, pulmonary
function and cytokines, we will evaluate the action of these Chinese medicine components & their
compounds for COPD rats’ oxidative stress & airway remodeling, explore the efficacy mechanism, and
expect to provide a theoretical basis of the clinical application about Chinese medicines for COPD.
Methods: (1) 220 male SD rats were randomly divided into 22 groups (n = 10). Group A: normal control
group; Group B: COPD model group; Group C: COPD model + NAC group (800mg/(kg)); Group D: COPD
model + Dexamethasone group(0.1mg/(kg.d)); Group E: COPD model + low-dosage-Icariin
group(10mg/(kg.d)); Group F: COPD model+ middle-doseage-Icariin group(20mg/(kg.d)); Group G:
177
COPD model +high- doseage- Icariin group(40mg/(kg.d)); Group H: COPD model + low-dosage
-Astragaloside group(4mg/(kg.d)); Group I: COPD model+middle-doseage -Astragaloside group
(8mg/(kg.d)); Group J: COPD model +high-doseage -Astragaloside group(16mg/(kg.d)); Group K: COPD
model + low-dosage -Catalpol group(10mg/(kg.d)); Group L: COPD model+middle-dosage-Catalpol
group(20mg/(kg.d)); Group M: COPD model+high-dosage-Catalpol group(40mg/(kg.d)); Group N: COPD
model+low-doseage-Baicalin group(20mg/the (kg.d)); Group O: COPD model+ middle-dosage-Baicalin
group(40mg/(kg.d)); Group P: COPD model+high-doseage-Baicalin group(80mg/(kg.d)); Group Q:
COPDmodel+low-dosage-Astragaloside+low-dosage-Icariin+low- dosage-Catalpol; Group R: COPD
model+middle-dosage-Astragaloside+middle-dosage-Icariin +middle-dosage-Catalpol; Group S: COPD
models+high-dosage-Astragaloside+high-dosage-Icariin+ high-dosage-Catalpol; Group T: COPD
model+low-dosage-Astragaloside+low-dosage-Icariin+low-dosage-Catalpol+low-dosage-Baicalin;
Group U:
COPD model+middle-dosage-Astragaloside+middle-dosage-Icariin+middle-dosageCatalpol+middle-dosage-B aicalin; Group V: COPD models+high-dosage-Astragaloside+high-dosageIcariin+high-dosage-Catalpol+high-dosage-Baicalin. (2) COPD animal model was established by
cigarette smoke exposure. (3) The rat lung function was checked by the Buxco spirometer. (4) The
concentrations of IL-8, IL-10, TNF-α, TGF-β1 and ET-1 in BALF were measured by enzyme-linked
immunosorbent assay. (5) The concentrations of IL-8, IL-10, TNF-α, TGF-β1, ET-1, MMP-1, MMP-9 and
TIMP-1 in serum were measured by enzyme-linked immunosorbent assay. (6) Using biochemical
methods to detect the concentrations of GSH, SOD, CAT, HO-1, and MDA in the lung tissue
homogenates; (7) Tissue was fixed by formalin from right lung. Sagittal sections were stained with
hematoxylin-eosin, and observed for the bronchus and lung histopathological changes. (8) The altering of
inflammatory cell infiltration under bronchial mucosa, the number of fibroblasts, the thickness of bronchial
smooth muscle in 200m, the thickness of smooth muscle in small pulmonary arteries in 200m, the
mean pulmonary artery wall area, and the average alveolar area were observed with pathological image
analysis instrument. Results: (1) COPD model was made successfully in SD rat. Lung function test
showed that FEV0.1/FVC (%), PEF, and MMEF were significantly lower in the group with COPD than the
normal control group (P <0.05); FRC of the COPD group was significantly higher than that of the normal
control group (P <0.05). In the Dexamethasone group, there was no significant improvement in lung
function and no statistical significance (P>0.05), compared with the model group.Lung function in the NAC
group, the Icariin group, the Astragaloside group, the Catalpol group, the Baicalin group and the
compounds , was significantly improved compared with the model group, and it enhanced with the
increasing of the dose(P <0.05). (2) HE staining pathological section of lung tissue in model group
showed alveolar septa destruction, enlargement of alveolai, infiltration of alveolar macrophages and
neutrophils, and increasing of goblet cell. Lung tissue inflammation grade, the number of fibroblasts,
thickness of bronchial smooth muscle, thickness of pulmonary artery smooth muscle, mean pulmonary
artery wall area, and the average alveolar area in model group were significantly higher than the normal
group (P<0.01). Compared with the model group, the pathological ex-tent of the damage in the
Dexamethasone group was not improved markedly (P>0.05). However, the pathological changes
lessened gradually after interventio-n of NAC, Icariin, Astragaloside, Catalpol, Baicalin and compounds,
as well as the proliferation & the degree of inflammation, and they became near to nor-mal while the dose
rised gradually. (3) Compared with the normal group, the levels of serum IL-8, TNF-α, TGF-β1, ET-1,
MMP-1, MMP-9, TIMP-1 in the COPD model group were significantly increased (P<0.05 or P<0.01), but
suppression inflammatory cytokine IL-10 reduced obviously (P <0.05). Compared with the model group,
the levels of serum IL-8, TNF-α, MMP-9, TGF-β1 in Dexamethasone group were not significantly different
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(P>0.05), but the levels of serum MMP-1, TIMP-1, and ET-1 were clearly lower than that of the model
group (P <0.05), and IL-10 rised significantly(P <0.05). Compared with the model group, the serum levels
of IL-8, TNF-α, TGF-β1, ET-1, MMP-1, MMP-9, TIMP-1 in the NAC group, the Icariin group, the
Astragaloside group, the Catalpol group, the Baicalin group and the compound groups gradually reduced
with the dose incresed, serum IL-10 rised with the dose increased. At the highest doses, they all were
statistically significant(P<0.05 or P<0.01). (4) Compared with the normal group, the levels of IL-8, TNF-α,
TGF-β1 and ET-1 in BALF in the model group were significantly increased, while the IL-10 decreased
obviously(P <0.05 or P <0.01). Compared with the model group, the levels of IL-8 and ET-1 in BALF
significantly decreased in the Dexamethasone group (P <0.05), but TNF-α and TGF-β1 did not go down
significantly (P> 0.05), and IL-10 rised significantly(P <0.05). Compared with the model group, the levels
of IL-8, TNF-α, TGF-β1 and ET-1 of BALF in the NACgroup, the Icariin group, the Astragaloside group, the
Catalpol group, the Baicalin group and the compound groups gradually reduced with the dose incresed,
but IL-10 went up with the dose added. At the highest doses, they all were statistically significant (P<0.05
or P<0.01). (5) Compared with the normal group, the MDA level of lung homogenate in the model group
was significantly increased (P <0.05), and the level of SOD, CAT, HO-1 significantly reduced (P <0.05),
while the level of GSH decreased slightly (P> 0.05). Compared with the model group, the MDA level of
lung homogenate in the dexamethasone group significantly decreased (P<0.05), however, the levels of
CAT, GSH, SOD and HO-1 in lung homogenate were not significantly improved (P> 0.05). Compared with
the model group, the MDA level of lung homogenates in the NAC group, the Icariin group, the
Astragaloside group, the Catalpol group, the Baicalin group and compound groups were significantly
decreased, while the levels of SOD, CAT and GSH, HO-1 rised significantly. At the highest dose, they
were statistically significant (P<0.05). Conclusion: 1) After six months of passive smoking, we
successfully copied the rat COPD airway remodeling model. 2) COPD model group rats manifested
severe oxidative/antioxidant imbalance. 3) Icariin, Astragaloside, Catalpol, Baicalin and their compounds
characterized with anti-inflammatory and antioxidant capacity, and could improve lung function and
airway remodeling in COPD rat. When given medium or high dose of Icariin, Astragaloside, Catalpol,
Baicalin for COPD rat, their efficacys were equal to or better than the NAC. 4) NAC had a strong
anti-inflammatory and antioxidant role, and could effectively improve the airway remodeling.
Dexamethasone’ efficacy in COPD was not obvious, and long-term use of hormones would accompany
with a lot of side effects.
A-235
The research on the relationship between compatibility of Buzhongyiqi Decoction and
immune regulation
Luo Qingli 1, Dong Jingcheng 1, Guo Xin 2*, Yang Yang2
1Affiliated Huashan Hospital, Fudan University, 200040, Shanghai, China;2 Shanghai University of Traditional Chinese
Medicine , 201203, Shanghai, China: *Correspondence should be addressed to E-mail: guoxin0112@yahoo.com.cn
179
Objective: To analyze the different compatibility value and the regulation of immune effect of Buzhongyiqi
Decoction. Method: CTX was injected into rats’ abdomen to make rat model of poor immunity. We chose
spleen index, thymus index, splenic lymphocyte proliferation test and Quantitative Hemolysis
Spectrophotometry to detect the medical effects among Buzhongyiqi Decoction, each herbal medicine in
this decoction and immune regulation. Result: Both Decoction and each herbal medicine in the decoction
have the functions of strengthening the non-specificity, cell and humor immunity of poorly immuned rats in
different levels（
P<0.05，
P<0.01）
. Conclusion: Different compatibility value and doses could have
different effects on immune pesticide effect.
A-236
A comparison of the serum and sputum inflammatory mediator profiles in asthma and
COPD
Cao Yuxue #, Gong Weiyi #, Zhang Hongying, Liu Baojun, Li Bei, Wu Xiao, Duan Xiaohong *, Dong
Jingcheng *
Institute of Integrated Traditional Chinese and Western Medicine, Department of Integrated Traditional Chinese and Western
180
Medicine, Huashan Hospital, Fudan University. 12 Middle Wulumuqi Road, Shanghai, P. R. China. #Contributed equally,
*Correspondence: Professor Xiaohong Duan (E-mail: njmu@sohu.com, Fax: 86-21-52888265) and Professor Jingcheng Dong
(E-mail: jcdong2004@126.com, Fax: 86-21-52888265).
Objective: Cytokines play a vital role in orchestrating the inflammation in asthma and COPD. This study
aimed to investigate the serum and sputum cytokine pattern in asthma and COPD patients. Methods:
Clinical characteristics, blood and sputum in asthma, COPD subjects and healthy controls were collected.
Blood and sputum processing was performed for serum and sputum supernatant respectively. Cytokines
were measured by enzyme-linked immunosorbent assay (ELISA) and inflammatory antibody array.
Results: Assess by ELISA, only a few cytokines expression increased in COPD group compared to
healthy subjects, while measured by antibody array more mediators showed differentiation. In the
analysis of sub-phenotypes, cytokines differential expression were more significant between eosinophilic
and non-eosinophilic airway inflammation than between asthma and COPD, showed both in the results
measured by ELISA and antibody array. However, little difference was found in subgroups between
smoking and non-smoking subjects. Conclusions: Both conventional differentiation, asthma and COPD,
and the novel taxonomy, eosinophilic and non-essinophilic airway inflammation may reveal the differential
expression of inflammatory mediators, making clinicians understand the cytokines network of airway
inflammation better.
A-237
A new type health food supplement with analgesic and Anti-inflammatory Activities,
ard-p401, but without adverse reactions
Michio Katagi1，
Akio Yasukawa2，
Chiaki Kudoh3，
Yasuhiro Komatsu4，
Eiichi Hujihira5
1Kawagoe Animal Medical Center, Saitama, Japan; 2Nishiogi Animal
Hospital, Tokyo, Japan; 3Kudo Chiaki Hospital, Japan; 4Sun
R and D Institute for Natural Medicines Co.Inc.Ginza, Japan; 5College of Hokkaido Pharmaceutical Science, Hokkaido, Japan
In Japan, around 25% of the population is over 60 year old people. In aged people, osteoarthritis and
dislocation of the backbone cartilage induce defect of the locomotive activity and reduction in Quality of
Life (QOL). The non steroidal anti inflammatory drugs (NSAIDs) has been taking for a therapeutic
treatment of the diseases. NSAIDs, however, induce the well known adverse reactions such as peptic
gastric ulcer. Therefore, we need safer medicines that are allowed to use for long-term therapy without
any adverse reactions in digestive organs. is uncertain even their approved safety. Then, development of
a new type of a health food supplement that consists of foods and produce anti-inflammatory and
analgesic activities would be requested. The authors have already developed a new health food
supplement, SRD- P001 (Sun R&D Institute for Natural Medicines Inc. Ltd. in Tokyo) which contains
ginger, western white willow, cinnamon, bitter orange, glucosamine and so on. These plants (crude drugs)
show anti inflammatory, analgesic effects, and anti oxidative activity. In this time, we would like to present
the efficacy of SRD-P401 (a modified SRD-P001) that is a newly developed health food supplement with
anti CoxⅠand CoxⅡ
, sedative and analgesic, anti-inflammatory and, anti-oxidant action, on dogs and/or
cats with joint diseases. In order to know the clinical efficacy (pain control, anti inflammatory activity) of
SRD-P401, it was assessed by giving to patient dogs and cats with intervertebral spondylopathy,
osteoarthritis, postoperative pain control. SRD-401 exhibited anti pain and anti inflammatory activities
181
with no side effects. These results indicated that it could be a useful health food supplement in the
veterinary medicine.
A-238
A study of 406 cases with fever in the year between March 2009 and March 2010
Li Zheng 1, Zhang Yanxin 1, Li Fengsen1*
1Affiliated
Traditional Chinese Medicine Hospital Xinjiang Medical University, 830000, Urumqi, Xinjiang Uighur Autonomous
Region China; *Correspondence should be addressed to fengsen602@163.com
Objective: To investigate the etiology, diagnostic methods and procedure for management with fever
diseases of the respiratory system. Methods: All 406 cases of respiratory disease with fever admitted to
the respiratory department of Chinese Traditional Medical Hospital of Xinjiang between March 2009 and
March 2010.Medical records were reviewed retrospectively. Results: There were 225 male patients and
181 female patients with an average age of 45. 34 years old. 385 cases had infectious disease with 183
cases of pneumonia; 139 cases of acute upper respiratory tract infection; 27 cases of tuberculous
pleuritis; 28 cases of pulmonary tuberculosis; 21 cases of acute treacheobronchitis; 5 cases of lung
abscess; 31 cases of acute exacerbations of chronic obstructive pulmonary disease; 21cases of
bronchiectasis; 21 of the 406 cases had non-infectious disease with 9 case of lung cancer; 11 cases of
interstitial pneumonia; 1 cases of extrinsic allergic alveolitis. The fever pathogenesis of tuberculosis is
longer then other infectious diseases. the level of fever of tuberculosis is lower then other infectious
diseases. there are no difference between infectious disease and non-infectious disease. In these 406
cases, 128 cases (31. 52%) can give final diagnosis by normal clinical data, 221 cases (54. 31%) need CT
examination to concluded; there are 9 1cases need pathology examination to identify; other 48 cases (11.
82%) have to diagnose by the result of test cure. Conclusions: Infectious diseases were the major
causes of respiratory disease with fever. The common reasons are pneumonia; acute upper respiratory
tract infection; tuberculosis; acute exacerbations of chronic obstructive pulmonary disease. Xinjiang has a
high incidence rate of Tuberculosis, it should be reconstructed in diagnosis. Common diseases can be
diagnosed by the clinical data, CT examination is very important in the diagnosis of the respiratory
disease with fever, pathology examination is accurate, and the test of cure is an obbligato method for the
diagnosis of Tuberculosis.
A-239
Anti-inflammatory and endocrine-regulated effects of icariin on cigarette smoking
induced chronic obstructive pulmonary disease in rats
Zeng Lixuan #, Gong Weiyi #, Wu Jinfeng, Zhang Hongying, Liu Baojun, Cao Yuxue, Du Yijie, Duan
182
Xiaohong *, Dong Jingcheng *
Institute of Integrated Traditional Chinese and Western Medicine, Department of Integrated Traditional Chinese and Western
Medicine, Huashan Hospital, Fudan University. 12 Middle Wulumuqi Road, Shanghai, P. R. China. #Contributed equally,
*Co-correspondence: Professor Xiaohong Duan (E-mail: xhduan2012@126.com; Fax: 86-21-52888265), Professor Jingcheng
Dong (E-mail: jcdong2004@126.com; Fax: 86-21-52888265).
Background: Chronic obstructive pulmonary disease (COPD) is a major health problem worldwide, and
herbal medicines have a long history of experience and proven successful treatment for COPD. However,
little information is available on the effect of icariin on cigarette smoke (CS) induced COPD. Objectives:
To investigate the effects of icariin administration on CS-exposed COPD rats. Methods: COPD rat model
was established by CS exposure. Thirty-six rats were randomly assigned to six groups: control, CS,
dexamethasone (DXM), and icariin (20 mg/kg, 40 mg/kg, 80 mg/kg). The lung pathology was observed
and leukocytes in bronchoalveolar lavage fluid (BALF) were counted by optical microscope. Pulmonary
function was measured by using an animal plethysmograph. The levels of cytokines and hormornes were
measured both by enzyme linked immunosorbent assay (ELISA) and reverse transcription polymerase
chain reaction (RT-PCR). The expression of NF-κB p65 protein were detected by immunohistochemistry.
Results: Abnormalities of pulmonary function and pathology were observed in CS-induced COPD rats,
and CS exposure significantly increased expression of pro-inflammatory mediators in serum and BALF,
down-regulated levels of corticotropin releasing hormone (CRH) and corticosterone (CORT) in tissue and
enhanced NF-κB p65 expression in the lungs. Icariin administration significantly protected pulmonary
function and attenuated the CS-induced inflammatory response by reducing inflammatory cells and
production of IL-8, IL-6 and TNF-α. In addition, icariin showed endocrine-regulated effect by increasing
CRH and CORT expression. These results were not found in rats treated with DEX. Conclusions: Icariin
has anti-inflammatory and endocrine-regulated effects in CS-induced COPD rat model and its
effectiveness enhances with the increase of dosage.
A-240
Anti-inflammatory effects of icariin on cigarette smoke induced inflammatory responses
in mice
Li Lulu1, Wu Jinfeng 1, Liu Baojun 1, Sun Jing 1, Gong Weiyi 1, Lv Yubao1, Zhang Hongying 1, Luo Qingli 1,
Du Wenjing 1, Xu Hailin 1, Dong Jingcheng *
1Department
of Integrative Medicine, Huashan Hospital, Fudan University, 12 Middle Urumqi Road, Shanghai 200040, China;
*Corresponding author: Tel.: +86 21 52888301; Fax: +86 21 52888265; E-mail address：
jcdong2004@hotmail.com
The present study was conducted to investigate the effects of icariin, a major constituent of flavonoids
isolated from the herb Epimedium, on cigarette smoke (CS) induced inflammatory responses in mice.
BALB/c mice were exposed to smoke of 15 cigarettes for 1 h/day, 6 days/week for 3 months and dosed
with icariin (25, 50 and 100 mg/kg) or dexamethasone (1 mg/kg). We found that icariin significantly
protected pulmonary function and attenuated CS-induced inflammatory response by decreasing
inflammatory cells and production of TNF-α, IL-8 and MMP-9. Further studies revealed that icariin
administration markedly restore CS-reduced GR protein expression, which might subsequently contribute
to the attenuation of CS-induced respiratory inflammatory response. Together these results suggest that
icariin has anti-inflammatory effects in cigarette smoke induced inflammatory models in mice, possibly
183
achieved by modulating GR protein expression.
A-241
Application of the Delphi method of development of chronic obstructive pulmonary
disease "fast"、
"slow" criterion
Li Fengsen 1,2*, Gao Zhen 2, Jing Jing2, Xu Dan2
1Department
2Xinjiang State
of Respiratory ，
Affilated Traditional Chinese Medicine Hospital of Xinjiang Medical University；
Research Center of Traditional Chinese Medicine, Urumqi, 830000, Xinjiang Uighur Autonomous Region ， China ；
*Correspondence should be addressed to fengsen602@163.com
Objective: Initial application of the Delphi method in patients with chronic obstructive pulmonary disease
progression fast, slow to judge basis. Method: Determine the development of chronic obstructive
pulmonary disease to determine the speed of evaluation, the main indicators and secondary
sub-indicators, the development of expert advice table, using the Delphi method, 50 experts carried out a
questionnaire consultation, mainly related to clinical respiratory specialists and related medical
professionals, from 12 states Xinjiang, urban areas, with good representation of the subject. Results:
Develop a course and grade as the main indicators of lung function, a positive coefficient of 90% of the
experts, the development of rapid indicators to determine dyspnea index score ≥3 than the maximum
76%, slow development of indicators to determine the level of lung function ≤Ⅱ
score than the maximum 69
%, concentration of expertise, coordination is good, desirable prediction, two questionnaire survey 24
indicators selected 11 indicators. Conclusion: Delphi method to develop COPD in the initial development
of "fast" "slow" to judge based on a certain role, duration, lung function levels, the annual number of
hospitalizations, CAT score could be used as disease progression in patients with COPD, "fast"、
"slow"
basis.
A-242
Baicalin is anti-inflammatory in cigarette smoke-induced inflammatory models in vivo
and in vitro: A possible role for HDAC2 activity
Li Lulu1, Wu Jinfeng 1, Liu Baojun1, Sun Jing 1, Gong Weiyi 1, Lv Yubao 1, Zhang Hongying 1, Luo Qingli 1,
Wu Xiao 1, Dong Jingcheng 1*
Department of Integrative Medicine, Huashan Hospital, Fudan University, 12 Middle Urumqi Road, Shanghai 200040, China
*Corresponding author: Fax: +86 21 52888265. E-mail address: jcdong2004@hotmail.com (J. Dong).
Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory disease characterized by
airway obstruction and progressive lung inflammation, which is insensitive to corticosteroids therapies. In
this study, we investigated the mechanism underlying the attenuation of cigarette smoke (CS)-induced
respiratory inflammation by baicalin, a flavonoid compound isolated from the root of Scutellaria
baicalensis Georgi, in vivo and in vitro. In vivo, mice were exposed to smoke of 15 cigarettes for 1 h/day,
6 days/week for 3 months and dosed with baicalin (25, 50 and 100 mg/kg) or dexamethasone (1 mg/kg).
In vitro, A549 cells were incubated with baicalin (10, 50 and 100 μM) or dexamethasone (10−12, 10−10,
10−8 and 10−6 M) followed by treatments with cigarette smoke extract (CSE, 2.5 and 5%), or TNF-α (10
ng/ml), or trichostatin A (TSA, 100 ng/ml). We found that baicalin significantly protected pulmonary
184
function and attenuated CS-induced inflammatory response by decreasing inflammatory cells and
production of TNF-α, IL-8 and MMP-9. This result was not found in the group treated with
dexamethasone. Baicalin also showed efficacy in enhancing histone deacetylase (HDAC)2 activity and
protein expression, however, it did not affect HDAC2 mRNA. Further studies revealed that baicalin
inhibited HDAC2 phosphorylation, suggesting that it may directly affect the protein structure and effect by
modification at post-translational level. Together these results suggest that baicalin has anti-inflammatory
effects in cigarette smoke induced inflammatory models in mice and A549 cells, possibly achieved by
modulating HDAC2.
A-243
Calcineurin/NFAT signaling pathway mediates ET-1-induced proliferation by regulating
phosphodiesterase-5 (PDE5) in pulmonary artery smooth muscle cells
Lu Jiamei1, Xie Xinming1, Han Dong1, Li Shaojun1, Li Manxiang1*
1Respiratory
Diseases Research Center, The Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an
710004, China; Corresponding author: LI Man-xiang, E-mail: manxiangli@hotmail.com
Objective: To examine whether Calcineurin/NFAT signaling pathway leads to ET-1-induced proliferation
of pulmonary artery smooth muscle cells (PASMCs) by regulating phosphodiesterase-5 (PDE5) and
whether the selective calcineurin inhibitor Cyclosporine A(CsA) or PDE5 inhibitor Sildenafil suppresses
ET-1-induced PASMCs proliferation. Methods: Endothelin-1(ET-1) was applied to stimulate PASMCs
proliferation with or without prior treatment of cells with the selective calcineurin inhibitor Cyclosporine
A(CsA) or PDE5 inhibitor Sildenafil. Calcineurin activity was measured using a calcineurin activity assay
kit. PDE5 expression was examined using immunoblotting. cGMP level was detected using a cGMP direct
immunoassay Kit. PASMCs proliferation was determined using a [3H]thymidine incorporation assay.
Results: ET-1 caused a 2.05-fold increase in the calcineurin activity, a 1.80-fold increase in PDE5
expression, a 3.20-fold increase in the DNA synthesis rate，
and reduced the cGMP level by 67%. While
CsA reversed ET-1-induced above effects. Inhibition of PDE5 by Sildenafil abolished ET-1-induced
reduction of cGMP level. Either CsA or sildenafil suppressed ET-1 stimulated PASMCs proliferation.
Conclusion: Activation of calcineurin/NFAT signaling pathway mediates ET-1-induced PASMCs
proliferation by stimulating PDE5 expression which further degrades cGMP. The selective calcineurin
inhibitor CsA or PDE5 inhibitor sildenafil suppresses ET-1-stimulated PASMCs proliferation.
A-244
Catgut implantation at acupoint on the effects of infertility of PCOS rats
Tian Yali 1, Yin Gaizhen 1
1 Affilated Traditional Chinese Medicine Hospital of Xinjiang Medical University，Urumqi, 830000, Xinjiang Uighur Autonomous
Region，China
Objective: observation points to thread burying polycystic ovary syndrome (PCOS) rats reproductive
endocrine hormone and ovarian the morphology of the influence, to investigate the PCOS model of the
possible role of rats mechanism, to provide the data for clinical research. Methods: 24 days of age female
SD rats subcutaneous injection androgen joint human gonadotropins (HCG) made mode for the legal
system in the rat model PCOS, the same period control injection saline. PCOS rats randomly divided into
185
model group, ClomiPhene group, embedding + Clomiphene group, embedding samples, among them,
the control group and the model group do not make any processing, other each byte is in 50 days of age
therapy since 20 days. After treatment, blood collection, using the united method for determination of
serum serum level of each index. Experimental animals in the afternoon with the male rat 6 according to
2:1 ratio and cage, in day 18 of death, observing the blastocyst implantation conditions, and gather the left
side ovarian, fixed in more than 4% of formaldehyde together, used as HE dyeing under light microscopy
tothe ovary influence. Results: The build, build module had significantly T higher than the control
group(P<0.05), with a statistical significance. After treatment, the model group, compared to the control
group, buried, buried quad line + CC group, CC group weight, serum T, LH, Leptin were significantly
reduced(P<0.05), all have significant difference; Each group FSH level difference was not significant
(P>0.05), no statistical significance. Conclusion: Meridians buried by adjusting the gonads axis line may,
in ovarian local coordination function, reduce the exceptional increasing LH and T level, at the same time,
reduce leptin levels, and promote the development and normal follicle ovulation.
A-245
Clinical value of serum procalcitonin and C-reactive protein in diagnosis of bacterial
infections
Wei Yanrong1, Yang Huiqing1*
1
Affilated Traditional Chinese Medicine Hospital of Xinjiang Medical University, Urumqi, 830000, Xinjiang Uighur Autonomous
Region，China
Objective: To evaluate clinical value of serum procalcitonin and C-reactive protein in diagnosis of
bacterial infections. Methods: 48 cases of patients with bacterial infections as the infection group, and
45 cases of non-patients bacterial infection patients as control group, the two groups of serum PCT and
CRP results were determined and had a comparative analysis. Results: 1. The infection group serum
PCT level was significantly higher than that of the control group (P<0.05): the positive rate of PCT was
93.8% in the infection group, which was higher than that of the control group(8.9%)(P<0.05);2. Serum
CRP level in the infection group was significantly than that of the control group(P<0.05).The positive rate
of CRP was 35.4% in the infection group, control group(20.0%),but no significant difference between the
two groups(P>0.05);3 The sensitivity, specificity, positive predictive value, negative predictive value and
diagnostic rate in the PCT diagnostic for serious bacterial infections sensitive diagnostic is higher than
that of the CRP, monitoring serum PCT levels can be used as a specific indicator of serious bacterial
infection, which has guiding significance for clinical diagnosis and treatment.
A-246
Comparison of standard sample preparation methods with real time fluorescence
quantitative PCR of Bacteroides thetaiotaomicron in intestinal from db / db type 2
diabetic mellitus mice
186
Yi Jinyang 1, Wang Ye 1, Zhu Ming 2, Han Xue 1, Yang Zhenzhen 1, Ji Fengcai 3, Zhen Shutao 4, Mao
Xinmin 5, Wang Lifeng 6, Ma Xiaoli 7, Li Linlin 1*
1Department
of Pharmacology, Xinjiang Medical University, Urumqi 830011, China; 2Department of Immunology, Xinjiang
Medical University, Urumqi 830011, China; 3Animal experiment centor, Xinjiang Medical University, Urumqi 830011, China;
4First
Affiliated Hospital, Xinjiang Medical University, Urumqi 830011, China; 5College of Traditional Chinese Medical, Xinjiang
Medical University, Urumqi 830011, China; 6Department of Physiology, Xinjiang Medical University, Urumqi 830011, China;
7Department
of Analysis and Testing Center ,Xinjiang Medical University, Urumqi 830011, China; *Correspondence should be
addressed to yz-lll@vip.sina.com
Objective: This study is to compare the enteric bacteroid content by using real-time fluorescent
quantitative PCR with two method of standard preparation in the db/db mice. Methods: To collect the
intestinal fecal and extract the microbial DNA, and use the V6 District of 16SrRNA Bacteroides
thetaiotaomicron primers to do the RT-qPCR and assay the content of intestinal Bacteroides
thetaiotaomicron. We selected the ATCC29148 Bacteroides thetaiotaomicron as a standard strain.
Results: The number of Bacteroides thetaiotaomicron in type 2 diabetic mellitus mice db / db group and
control db/m group by RT-qPCR, respectively, were 5.23×106±2.30×106, 1.52×107±3.23×106;
5.23×106±2.25×106, 1.45×107±3.39×106. Compared with the control group of db/m, the number of
Bacteroides thetaiotaomicron of db /db mice were increased（
P<0.05）
. The same results were got from the
standard bacteria method and normal group high positive method. Conclusion: Standard bacteria
method and normal group high positive method for quantitative detection of Bacteroides thetaiotaomicron
have no significant difference in mice , so the two kinds of detection methods can be used to detect the
level of intestinal flora.
A-247
COPD xide measurement and influencing factors
Wang Nana 1, Li Fengsen1*, Bai Wenmei 1, Zhai Yujiang 1
1
Affilated Traditional Chinese Medicine Hospital of Xinjiang Medical University Urumqi, 830000, Xinjiang Uighur Autonomous
Region，China；*Correspondence should be addressed to fengsen602@163.com
Objective: Study of patients with COPD, asthma, normal human exhaled nitric oxide ( fractional exhaled
nitric oxide, FeNO ) determination of mean and its influencing factors. Methods: Selection of patients with
COPD, asthma patients, 30 normal subjects were used in Sweden, Niels ( NIOX ) " exhaled nitric oxide
measurement system for determination of the FeNO value ". Results: In patients with chronic obstructive
pulmonary disease FeNO mean was 24.7 ± 14.5ppb, FeNO of asthmatic patients mean was 63 ±
50.7ppb, FeNO of normal mean was 18.7 ± 6.2ppb; three groups of subjects in weight was not
significantly different ( P > 0.05), age, height, body mass index, FEV1, FEV1/FVC were significant
difference ( P <0.05 ). Conclusion: Asthma, COPD patients and FeNO values were higher than those of
normal subjects, and no significant difference in body weight, three groups of subjects in the age, height,
body mass index, FEV1, FEV1/FVC all have differences, but with the FeNO value determination of no
linear correlation between the consideration for sample volume due to less, further study.
A-248
Correlation analysis on acute exacerbating COPD patients FENO and pulmonary function
187
Zhai Yujiang 1, Li Fengsen 2*, Xu Qian 1, M.Huan 2, Su Jun 2
1Xinjiang
Medical University Post-graduate College; 2Affilated Traditional Chinese Medicine Hospital of Xinjiang Medical
University Urumqi, 830000, Xinjiang Uighur Autonomous Region ， China ； *Correspondence should be addressed to
fengsen602@163.com
Objective: To make analysis on the acute exacerbating COPD patients FENO and the related factors of
pulmonary function. Method: Select the hospitalized 70 AECOPD patients between 48 and 89 years old
as the research object to collect the general information (name、
gender、
age、
nationality、
height、
weight and body mass index), complete the questionnaire of medical history、
allergic history、
smoking
history and asthma history, make tests with Sweden NIOX nitric oxide tester, then record the detected
pulmonary fractions including FEV1% and FEV1/FVC%. Results: ⑴
The average FENO fraction of 70
AECOPD patients is 26.4  16.64ppb, the minimum fraction is 7.1ppb, and the maximum fraction is
99.6ppb；
⑵
The FENO fraction in different age groups has the statistical difference(P＜
0.05). As people grow
older, the FENO fraction is gradually reduced；
⑶
The FENO fraction has no statistical difference among
different weight index groups(P＞
0.05). As the weight index becomes bigger, the FENO fraction is
gradually reduced；
⑷
The FENO fraction has the statistical difference among the pulmonary patients in
different degree(P＜
0.05). As the patient's pulmonary function becomes declined, the FENO fraction is
gradually reduced; ⑸
The FENO fraction is negatively related with age(r=-0.111,P=0.047),
BMI(r=-0.098,P=0.042) and FEV1/FVC%(r=-0.002,P=0.044). Conclusions: FENO detection plays an
important role for the judgment of airway inflammation for COPD patients.
A-249
CPPE (Compound of Pomegranate Peel Extraction) Reduce Adipogenesis Through
Inhibit Mitotic Clonal Expansion
2
Wang Lifeng 1, Tao Yicun 1, Zhang Minfang 1, Jiao Yi 1, Luo Xin 1, Li Linlin , Mao Xinmin1*, Ren Jun 1
1Pharmacology
Department, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; 2Traditional Chinese Medicine
Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;
*Correspondence should be addressed to
mxm3277@sina.com
Obesity closely related to diabetes, cardiac diseases, inflammation and their associated disorders.
Current treatment of obesity includes various drugs which cause untoward side effects and inflict
economic burden. Medicinal plant products have been highlighted as an alternative management of
obesity to some extent. Xinjiang is colonized by different ethnic groups, and plant herb, such as:
pomegranate. Pomegranate extract bear a lot function: anti-inflammation, anti-proliferation,etc. In this
study, data show CPPE had no cyto-toxicity and reduced adipogenesis in 3T3-L1 cell line. CPPE is
required at the onset of differentiation for maximal inhibition. It reduced the cell number, especially within
2 days after classic adipogenic induction .Cell cycle analysis shows that cells differentiated with CPPE are
severely restrained from entering the S phase of the cell cycle. And also CPPE attenuate C/EBPαDNA
binding activity then inhibit PPAR-γand aP2 expression to influence fat accumulation in final stage. In one
word, the adipogenic reduction of CPPE might be through inhibition of early Mitotic clonal
expansion(MCE) and fat accumulation in final stage .
188
A-250
Different Type Certificate in Patients with Asthma who Body Fluids, and Peripheral Blood
of the Change of the Relevant Immune Index
Su Jun 1, Li FengSen 1,2,3*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;2Affilated Traditional
3Xinjiang State Research Center of Traditional Chinese Medicine,
Chinese Medicine Hospital of Xinjiang Medical University；
Urumqi, 830000, Xinjiang Uighur Autonomous Region ， China ； *Correspondence should be addressed to
fengsen602@163.com
Objective : According to the different type certificate in patients with asthma who saliva, sputum, nasal
secretions secretory in immunoglobulin A (SIgA) and blood CD4+, CD8+ T lymphocytes, eosinophilic
acidic cationic protein (ECP), serum total IgE (T-IgE) discussed were the attack of asthma mechanism, In
order to further understand the development of childhood asthma mechanism, and also summarizes
different card type asthma the changing characteristics of the relevant immune index, for Chinese
medicine to asthma on syndrome differentiation and classification provides an objective basis for
prevention and cure of bronchial asthma and provide theoretical basis. Methods: Collect different card
type 200 patients with asthma (asthma group) and normal 30 cases (normal group), using the method of
ELISA sputum, saliva and nasal secretions SIgA of the week outside the venous and ECP, T-IgE testing;
The flow cytometric the blood of CD4+, CD8+ T lymphocytes were detected. Results: (1) asthma in the
blood of CD4+, CD8+T lymphocytes, CD4+/CD8+ expression compared with normal group are increased,
a statistically significant (P<0.05); Different type certificate in patients with asthma who in peripheral blood
CD4+, CD8+, CD4+/CD8+ compared to no difference obvious (P>0.05). (2) asthma group of saliva,
sputum, nasal secretion of SIgA content are the normal group reduced significantly, and was statistically
significant (P<0.05); Different type certificate in patients with asthma who SIgA content found in saliva
compared to no significant difference was statistically significant (P>0.05); Different card type between
patients with asthma sputum SIgA content in comparison was statistically significant (P<0.05), cold
wheezing patients in the sputum SIgA content is higher than the wind wheezing, virtual wheezing sputum,
cold bag hot wheezing patients (P<0.05), and the rest between each group there were no significant
difference (P>0.05);Every syndrome type between patients with asthma in rhinal secretion SIgA content is
statistically significant compared (P< 0.05), cold wheezing patients in rhinal secretion SIgA content are
lower than the wind, cold hot sputum wheezing bag wheezing patients (P<0.05); The rest between each
group compared to no difference obvious (P>0.05). (3) every syndrome patients with type serum T-IgE,
ECP content is not statistically significant (P>0.05). Conclusion: (1)The bronchus asthma patients with
acute episode exist in immune function disorder; (2)Asthma patients in acute episode in the peripheral
blood immune index CD4+, CD8+T lymphocytes, T-IgE and phlegm, saliva, nasal secretion of SIgA
happens disorder, including the upper respiratory tract secretion of immune index SIgA change
particularly obvious;(3) According to Chinese medicine diagnosis and classification, cold wheezing type of
immune index in patients with asthma who most obvious change. So in asthma in the prevention and
control of the immune function of patients with asthma correct balance disorders especially important, to
the upper respiratory tract secretion of immune index test related will help understand the serious attack
of asthma in traditional Chinese medicine and in traditional Chinese medicine for asthma syndrome type
provide objective theoretical basis points.
189
A-251
Discrimination of Type 2 diabetic patients from healthy controls by GC metabonomics
method based on their serum fatty acid profiles
Ma Xiaoli1, Tian Lan 1, Li Linlin 2, Meng Lei 1, Wang Ye1, Mao Xinmin3*
1Analytical&
testing center, college of Pharmacy, Xinjiang Medical University, Urumqi, 830000, Xinjiang Uighur Autonomous
Region ，China;2Pharmacology Department, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China; 3Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region
, China;* Correspondence should be addressed to mxm3277@sina.com
Objective: To investigate the serum free fatty acid variation in diabetic patients and healthy volunteers in
order to explore the application of metabolic profiling analysis in research of diabetes. Methods: By
adding n-heptadecanoic acid as internal standard, the human serum were methylesterifiled by
H2SO4/CH3OH, extracted by n-hexane, FID detector, programmed. Results: 7 kinds of fatty acids can
be separated completely, with a good linear from 2~500ug/ml(r>0.999),the average recovery rate of three
level was between 76.6%~121.9%.the method can be used to discriminated 31 diabetes patients and 30
healthy volunteer. Conlusions: The results suggested that serum free fatty acid profiles determined by
capillary gas chromatography combined with pattern recognition analysis of the data might provide an
effective approach to the discrimination of Type 2 diabetic patients from healthy controls. And the
applications of pattern recognition methods have improved the sensitivity and specificity greatly. In this
study, metabonomics method was employed to the diagnosis of Type 2 diabetes mellitus (DM2) based on
serum lipid metabolites.
A-252
Distribution and Prevalence of Human Papillomavirus (HPV) in Uygur Women in China: A
Population-Based Genotype Study
L.Hua 1, G.Abdixkur, N.Amat 2, H.Yanchun, Z.Suqin, Z.Mutallip, Z.Feng, G.Kurban, T.Ablimit, F.Mijit,
G.Nayim, G.Abliz *
1The
fifth Department of gynecology of the affiliated
tumor hospital of Xinjiang Medical University, Xinjiang Urumqi
830011,China; 2Department of Traditional Uygur Institute, Xinjiang Medical University, Xinjiang 830011, China; *Correspondence
should be addressed to Gzlnr420@yahoo.com.cn
Objective: Cervical cancer represents a major health problem in China as well as in other developing
countries. Infection with human papillomavirus (HPV) is the main cause of cervical cancer and its
precursor lesion, cervical intraepithelial neoplasia (CIN). HLAII DQB1 polymorphisms have been shown
to associate with cervical cancer risk, but results varied among different populations. We aimed to
investigate the relationships between the HLA-DQB1 allele polymorphisms and the Uygur women’s
susceptibility to cervical carcinomas. Methods: Cervical swabs were obtained from 190 cervical cancer
patients and 190 healthy controls; HLA-DQB1 genotyping was performed using a PCR-SSP protocol.
Results: The frequency of DQB1*03 was significantly lower among cervical cancer patients overall as
2
compared to controlsOR<1 (  ＝
6.079 P=0.014 OR=0.683), and the frequency of other HLA-DQB1
alleles have no statistical differences between the cervical cancer group and the control group.
Conclusion: This is the first report of HLA-DQB1 alleles associated with cervical carcinoma in Uygur
190
women. HLA-DQB1*03 allele may be the protective gene of the Uygur women with cervical cancer in
Kashgar and Hotan Region. The role of HLA alleles in the carcinogenesis of cervical carcinomas may play
a important role in the gene interference research of cervical cancer.
A-253
Effects of baicalin on airway remodeling in asthmatic mice
Sun Jing 1,2, Li Lulu 1,2, Wu Jinfeng 1,2, Liu Baojun 1,2, Gong Weiyi 1,2, Lv Yubao 1,2, Luo Qingli 1,2, Duan
Xiaohong 1,2, Dong Jingcheng 1,2*
1 Department
of Integrative Medicine, Huashan Hospital, Fudan University, Shanghai, China; 2 Institute of Integrated Traditional
Chinese and Western Medicine, Huashan Hospital, Fudan University, Shanghai, China; *Correspondence: Prof. Dr. Jingcheng
Dong, Department of Integrative Medicine, Huashan Hospital, Fudan University, Middle Urumqi Road 12#, Shanghai, China.
Email: jcdong2004@126.com Phone: 52888301 Fax: 52888301
Airway remodelling is an important characteristic of asthma, linking inflammation with airway
hyperresponsiveness (AHR). Baicalin, a major active compoment, is isolated from Radix Scutellariae.
Many studies show that baicalin has anti-inflammatory, anti-bacterial and anti-allergic effects. Here we
investigate the influence of baicalin on asthmatic airway remodeling and the mechanism underlining the
anti-remodeling effect in vivo. Asthmatic airway remodeling mice model was established by
ovalbumin(OVA) exposure. Seventy female BALB/c mice were randomly assigned to seven experimental
groups: blank, OVA, hexadecadrol, control and baicalin (25 mg/kg, 50 mg/kg, 100mg/kg) group.
Pulmonary function was measured using an whole body plethysmography in conscious and unrestrained
mice. The lung pathology was observed and measured. The production of cytokines in bronchoalveolar
lavage fluid (BALF) and serum was measured using enzyme-labeled immunosorbent assay(ELISA) kits
and the expression levels of transforming growth factor-β1(TGF-β1) and vascular endothelial growth
factor(VEGF) were detected by immunohistochemistry. The protein expression levels of
TGF-β1、
VEGF、
extracellular signal–regulated kinase(ERK) and p21ras were measured using western
bloting. The results show OVA exposure significantly increased the expression of interleukin-13 (IL-13) in
BALF and serum, and TGF-β1、
VEGF、
ERK and p21ras expression in the lungs. Baicalin attenuated the
effects of OVA significantly. It can be concluded that baicalin has significant anti-remodeling effect on
OVA induced asthmatic airway remodeling mice model by decreasing expression of TGF-β1、
IL-13、
VEGF
and inhibiting the activation of the ERK pathway.
A-254
Expression of aquaporin 1 increases proliferation and metastasis potential of lung cancer
Wei Xiaobai 1, Dong Jingcheng 1,2*
1Department of chinese integrative medicine, Huashan Hospital, Fudan University, Shanghai,
of Fudan University, Shanghai 200040, China;
3
China, 2 Shanghai Medical College
Department of Integrated TCM and Western Medicine, Huashan Hospital
Affiliated Fudan University, Shanghai, China *Correspondence: Prof. Jingcheng Dong, Lab of Integrative Medicine for Lung,
Inflammation and Cancers, Huashan Hospital, Fudan University, Shanghai 200040, China. Tel: 86-21-52888301; fax:
86-21-52888265. E-mail address: jcdong2004@126. com
Objective: To explore roles of AQP1 in lung cancer oncogenesis and development, the effects of AQP1
191
on lung cancer cell proliferation and migration and metastasis were investigated on the LLC and LTEP-A2
cell lines in vitro. Methods: Both LLC and LTEP-A2 Lung cancer cells with different AQP1 expression
were used to study cell proliferation and migration potential. cell proliferation and migration potential.
Immunofluorescent assay was used to detect AQP1 expression levels in Both LLC and LTEP-A2 cells.
Low level expression of AQP1 by different dose AQP1-siRNA, AQP1 levels were quantified by real-time
quantitative PCR and western blot analysis. Immunofluorescence, MMP-2、
MMP-9、
TGF-β、
EGFR and
further in vitro study including tumor cell migration and invasion, wound healing assays etc were
performed. Results: Tumor cell migration and invasion test, wound healing assay showed significantly
decreased migration, invasion and wound healing in AQP1 lower-expression cells vs
AQP1-neg-transfected group (control tumor cells). Moreover, our results showed that MMPs decresed
after AQP1-siRNA in two both cell lines. the higher doses of AQP1-siRNA,the lower level of
MMP-2/MMP-9 expression.However,AQP1 knockdown did not have a significant effect on the TGF-β and
EGFR. Conclusions: AQP1-facilitated lung cancer cell proliferation and migration, possibly through
decresing the expression of MMP-2、
MMP-9 in part.
A-255
Icariin attenuates airway inflammation and eosinophil infiltration by suppressing eotaxin
and CCR3+ production in acute and chronic mouse models of allergic asthma
Cai Cui 1, Du Wenjing 2,3, Li Lulu 2,3, Sun Jing 2,3, Lv Yubao 2,3, Du Yijie 2,3, Dong Jingcheng 2,3*
1
Hangzhou Red Cross Hospital, Hangzhou 310003, China; 2 Shanghai Medical College of Fudan University, Shanghai 200040,
China; 3 Department of Integrated TCM and Western Medicine, Huashan Hospital Affiliated Fudan University, Shanghai, China
*Correspondence: Prof. Jingcheng Dong, Lab of Integrative Medicine for Lung, Inflammation and Cancers, Huashan Hospital,
Fudan University, Shanghai 200040, China. Tel: 86-21-52888301; fax: 86-21-52888265. E-mail address: jcdong2004@126. com
Bronchial asthma is characterized by airway inflammation, airway hyperresponsiveness (AHR), and
leukocyte infiltration. Icariin is a major flavonoid isolated from the Epimedium koreanum Nakai. In the
present study, we investigated whether icariin attenuates airway inflammation and eosinophil infiltration
by suppressing eosinophil chemokine and chemokine receptor production in acute and chronic mouse
models of allergic asthma. Intraperitoneal treatment with icariin (50mg/kg) could decrease the serum
OVA-IgE level, lung inflammatory scores and the expression of inflammatory cytokines (GM-CSF, IFN-γ,
IL-5, IL-6 and TNF-α) in asthmatic mice. Icariin also reduced OVA-induced airway hyperresponsiveness
and eosinophil infiltration. The expressions of chemokines and chemokine receptors, especially of eotaxin
and CCR3, was also down-regulated in asthmatic mice after icariin treatment. These data suggested that
icariin could attenuate airway inflammation and eosinophil infiltration through the down-regulation of
eosinophilic chemokines and chemokine receptor expression, in particular eotaxin and CCR3 axis were
likely to be involved.
A-256
Icariin attenuates glucocorticoid insensitivity mediated by repeated psychosocial stress
on an ovalbumin-induced murine model of asthma
Li Bei 1, Duan Xiaohong 1, Wu Jinfeng, Liu Baojun, Du Yijie, Luo Qingli, Jin Hualiang, Gong Weiyi, Dong
192
Jingcheng *
1Dr.
Bei Li and Dr. Xiaohong Duan contributed equally to the present study. * Corresponding author. Tel/fax: +86 21 52888301.
E-mail address: jcdong2004@126.com (J. Dong). Department of Integrative Medicine, Huashan Hospital, Fudan University,
Middle, Urumqi Road 12#, Shanghai, 200040, China
Evidence shows that psychosocial stress exacerbates asthma, but there is little intervention to alleviate
negative effects of psychosocial stress on asthma. Using a murine model combined psychosocial stress
with allergic exposure, we investigated the role of icariin in attenuating the negative effects of stress on
ovalbumin-induced airway inflammation. The results indicated that icariin administered remarkable
increased activity in the center of the open field, reversed airway hyperresponsivenesss, reduced
inflammatory cytokines infiltration to lung and whole body and also in part recovered glucocorticoid
responsiveness. Furthermore, our data also showed that icariin significantly inhibited psychosocial
stress-induced increases of corticosterone and markedly increased GR mRNA and protein expression in
the lungs of mice exposed to both stress and allergen. Collectively, we speculate that inducing GR
modulation might be the potential mechanisms of icariin to facilitate corticosteroid responsiveness of
cytokine production and reduce the negative effects of psychosocial stress on ovalbumin-induced airway
inflammation.
A-257
Icariside II Induces Apoptosis of Melanoma Cells in vivo through the Downregulation of
Survival Pathways
Wu Jinfeng 1,2△, Xu Jinhua 2△, E.Eksioglu 3, C.Xianghong 3, Z.Junmin 3, N.Fortenbery 3, W.Sheng 3*, Jing
Chengdong1*
1 Department
of Integrative Medicine, Huashan Hospital, Fudan University, 12 Middle Urumqi Road, 200040, Shanghai, China; 2
Department of Dermatology, Huashan Hospital, Fudan University, 12 Middle Urumqi Road, 200040, Shanghai, China;
3
Department of Immunology, H.Lee.Moffitt Cancer Center & Research Institute, MRC4-east Room 3072C, 12902 Magnolia Dr,
Tampa, FL, USA, 33612; △These authors contributed equally to this work, should be considered as co-first author.
* Corresponding authors e-mail addresses: jcdong2004@hotmail.com, sheng.wei@moffitt.org
Objective: To evaluate the antitumor effects of icariside II (IS), isolated from Herba Epimedii, on in vitro
and in vivo models of melanoma and determine its mechanism of apoptosis. Methods: In vitro: Mouse
(B16) and human (A375, SK-MEL-5) melanoma cell lines were treated with IS at different concentrations
(0-100μM). Cell viability and proliferation was detected by WST-1 assay and with the xCELLigence
system, respectively. Apoptosis was measured by the annexin-V/PI flow cytometric assay. Western blot
was used to measure cleavaged caspase 3, survivin, P-STAT3, P-ERK and P-AKT. In vivo: B16 and A375
cells were injected subcutaneously into C57BL/6J and BALB/c-nu mice, respectively. After one week, IS
solution at (50mg/kg, 100mg/kg) was administered by intraperitoneal injection (i.p) three times for a week.
Tumor size was measured with an electronic digital caliper. Results: IS inhibited the proliferation of
melanoma cells in a dose- and time-dependent manner. Treatment of A375 cells with IS resulted in an
increased number of apoptotic cells mirrored by increases in cleavaged caspase-3 and a decrease in
survivin expression. IS significantly inhibited the activation of the JAK-STAT3 and MAPK pathways but
promoted an unsustained activation peak of the PI3K-AKT pathway. IS showed potent antitumor activity
against melanoma in vivo. Conclusion: IS dramatically inhibited the proliferation of melanoma cells in
193
vivo and in vitro through the regulation of apoptosis. These effects demonstrate the ability of IS to
effectively overcome the survival signals of tumor cells which support further preclinical evaluation of IS in
cancer as a new potential chemotherapeutic agent.
A-258
Idiopathic acute eosinophilic pneumonia, one case of clinical features of analysis
Zhou Linfu 1*, Zong Xiaofu 2
1First
Affiliated Hospital of Nanjing Medical University, Department of Respiratory Medicine, Nanjing, 210029, China; 2People's
Liberation Army 81 Hospital Department of Respiratory Medicine, Nanjing, 210029, China; *Corresponding authors: Zhou LinFu,
Email: lfzhou@njmu.edu.cn
Objective: To investigate the clinical characteristics of patients with idiopathic acute eosinophilic
pneumonia (IAEP). Methods: We analyzed one case who were treated IAEP patients with clinical
manifestations, imaging and pathological features, and review reports about the IAEP the literature.
Results: The patient was a 70-year-old female, cough, chest tightness, and intermittent fever, acute
course of the disease, severe hypoxemia. The chest CT showed migratory pulmonary infiltrates with a
small amount of pleural effusion. Diagnostic thoracentesis showed pleural fluid eosinophils accounted for
28%. Bronchoalveolar lavage fluid eosinophils accounted for 59%. CT-guided percutaneous needle
biopsy shows eosinophil lung infiltration. Conclusion: IAEP is a rare disease, misdiagnosed as severe
pneumonia, acute lung injury or acute respiratory distress syndrome, glucocorticoid sensitive cured
without recurrence, the prognosis is good.
A-259
Impact of psychosocial stress on airway inflammation and its mechanism in a murine
model of allergic asthma
Li Bei 1, Duan Xiaohong 1, Wu Jinfeng 2, Liu Baojun 2, Luo Qingli 2, Jin Hualiang 3, Du Yijie 3, Zhang
Hongying 3, Cao Yuxue 3, Dong Jingcheng *
Department of Integrative Medicine, Huashan Hospital, Fudan University, Middle, Urumqi Road 12#, Shanghai, 200040, China
(Bei Li, Xiaohong Duan, Jinfeng Wu, Baojun Liu, Qingli Luo, Hualiang Jin, Yijie Du, Hongying Zhang, Yuxue Cao, Jingcheng
Dong) * Corresponding author. Tel./fax: +86 21 52888301; E-mail address: jcdong2004@hotmail.com (J. Dong).
Objective: Physicians and scientists have already recognized the fact that psychosocial stress evokes
asthma exacerbation, however, the mechanism of how stress gets inside the body is little known. This
study was to observe the impact of psychosocial stress on airway inflammation and its mechanism in the
ovalbumin-induced asthmatic mice combined with social disruption stress. Methods: Thirty-six male
BALB/c mice were randomly divided into control group (no manipulation), asthma group (ovalbumin
(OVA)-induced), asthma plus social disruption stress group, and social disruption stress group. The open
field video tracking system from Med Associates was used to assess animal behaviors like the time mice
spent in the center of the open field test, ambulatory and vertical activity, and fecal boli counts. The
invasive pulmonary resistance (RL) and cdyn test system from Buxco was used to detect the pulmonary
function. Enzyme-linked immunosorbent assay (ELISA) method was used to observe OVA-Ig E, T-helper
type 2 cytokines (IL-4, IL-5, IL-13) and corticostrone in mice serum; T-helper type 2 cytokines (IL-4, IL-5,
194
IL-13, IL-6, TNF-α)in BAL fluid; IL-6 and TNF-αlevels in supernatant of splenocyte cultured in vitro.
Hematoxylin-eosin (H&E) staining was used to perivascular eosinophilia, peribronchiolar eosinophilia,
epithelial damage and oedema in lung histology. Cell count kit-8 assay (CCK-8) was used to detect cell
viability and evaluate the inhibitory effect of corticosterone to splenocyte proliferation induced by LPS.
Real time-PCR was used to observe GR mRNA expression in mice pulmonary tissue. Western blotting
was used to observe GR protein expression in mice pulmonary tissue. Results: The open field test
showed that combined exposure to allergen and repeated stress significantly shortened the time the mice
spent in the center of the open field (P<0.01), increased ambulatory activity (P<0.01) and the count of
fecal boli (P<0.01), but deceased vertical activity (P<0.01). Results from invasive pulmonary function
demonstrated that airway hyperresponsiveness was enhanced by psychosocial stress compared with
allergy exposure alone. The ELISA results showed that cytokines from serum and BAL fluid was
significantly increased (P<0.05). Moreover, the lung histology showed that infiltrated inflammatory cells
were significantly increased in both allergy exposure and stress experience mice compared with allergy
exposure alone (P<0.05). Interestingly, serum corticosterone was remarkably raised by psychosocial
stress (P<0.05). In addition, the inhibitory effect of corticosterone on TNF-γand IL-6 in LPS-stimulated
splenocyte cultures in vitro was diminished in the allergy exposure plus stress mice compared to the
allergy exposure mice. The CCK-8 test revealed that the inhibition effect of corticostrone on splenocyte
proliferation induced by LPS was significantly impaired in the stress mice with or without allergy exposure,
while no significant effect was observed in the control and allergy exposure groups. Furthermore,
expressions of GR mRNA and GR protein were significantly reduced in the lung tissues of allergic
exposure mice combined with social disruption stress (P<0.05). Conclusion: Social disruption stress
could promote anxiety behaviors; it could activate the hypothalamic–pituitary–adrenal (HPA), but
meanwhile enhance airway hyperresponsiveness and inflammation; and it could also impair
glucocorticoid sensitivity and its function in a murine model of asthma. The down-regulation of
glucocorticoid receptor induced by social disruption stress was in part associated with glucocorticoid
insensitivity, which leaded to the asthma exacerbation or the prolonged inflammation respons
A-260
Intestinal bacteria support HIV infection？
Shu Zhanjun 1, Li Fengsen 1, Dilinuer Tuerhong 1, Yang Chengxin 2, Wang Xiuling 1, Halmurat Upur 3*
1National
Chinese medicine clinical research base in Xinjiang National Chinese medicine clinical research base in Xinjiang,
Urumqi, Xinjiang Autonomous Region 830000, China; 2Xinjiang Uighur Autonomous Region Infectious Disease Hospital, Urumqi,
Xinjiang Autonomous Region 830000, China; 3Xinjiang Medical University, Urumqi, Xinjiang Autonomous Region 830054, China;
* Correspondence to: Halmurat@263.net
Since the 1950s, researches have gradually realized that the body's bacteria help fight infection by
crowding out potential pathogens. In the past decades, scientists have even begun to see our microbiota
as thick-and-thin allies. However, the influence of gut bacteria on human immunodeficiency virus (HIV) is
largely unraveled. Our review likely sheds light on the previously indistinct role of commensal microbiota
in retroviral pathogenesis. The delicate yet critical balance between this enormous bacterial population
and GIT is gradually destroyed alone with HIV incursion. The leakage into the systemic circulation of
bacterial and by-products, such as lipopolysaccharide (LPS) directly stimulate the innate immune system
through Toll-like receptors. As a result, TLR4 activation provokes production of IL-10, which mediates
195
immunological tolerance. So a tangible deduction is that intestinal microbes may have involved in
triggerring for replication and transmission of HIV, just like other retroviruses.
A-261
Metabonomic classification and detection of small molecule biomarkers of malignant
pleural effusions
Zhou Xianmei1, He Cuicui2, Liu Yumei2, Zhao Yang 1, Zhao Dan 2, Du Yun 2, Zheng Weiyi 3, Li Jianxin 2
1Department
of Respiratory Medicine, Affiliated Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing University of
Traditional Chinese Medicine, Nanjing 210029, China; e-mail: zhouxianmeijs@yahoo.com.cn; Tel & fax: +86 25-86617141;
2State
Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University,
Nanjing 210093, China; E-mail address: lijxnju@nju.edu.cn; Tel & fax: +86 25-83686419; 3Innov Pharma, Suite 1004, 10
Xinghou Road, Nanjing High-Tech Zone, Nanjing, 210061, China
Up to date, the most of research has been focused on the big molecules in pleural effusions and diagnosis
of malignant ones still remains challenging. In the present study, targeting at the small molecules as
potential biomarkers to predict the malignancy of the effusions, the metabolic profiles of 81 clinical pleural
effusions (41 malignant effusions with lung cancer and 40 benign ones) were investigated through a
NMR-based metabonomic approach. In 1H NMR analysis, total 10 small molecules in the effusions were
simultaneously determined. Significantly higher mean values of valine, lactate and alanine and markedly
lower levels of acetoacetate, trimethylamine-N-oxide (TMAO), α- and β-glucose were observed in
malignant pleural effusions compared with those in benign ones. DFA modeling of NMR spectra
subjected to a validation allowed the malignant effusions to be discriminated from benign ones with high
accuracy of 95.83% and 96.97% in the training and validation groups, respectively. Currently, the
conventionally clinical analyses on chemical constituents in effusions could not provide a reliable
prediction of malignancy of the effusions, the present results revealed that the small molecules might
serve as useful biomarkers for diagnosis of the effusions and the present NMR based metabonomic
approach provided a highly valuable potential to rapidly and sensitively predict the malignancy of the
pleural effusions.
A-262
Mitochondrial gene mutations in Uygur patients with type 2 diabetes mellitus in Xinjiang
S.Abuduryim 1, Nurmuhammat Amat 2*, Halmurat Upur 2*
1 First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China; 2Traditional Uygur Medicine Institute, Xinjiang
Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should be addressed to nur818@homail.com or
Halmurat@263.net
Objective: To study the correlation between the 7 SNPs (single nucleotide polymorphisms)
mitochondrial DNA tRNALeu (UUR) gene and ND1 gene in Uygur patients with type 2 diabetes in
Xinjiang. Method: A case-control study composed of 223 type 2 diabetes patients and 292 sex-matched
and ethnically matched control participants were enrolled in the study. Polymerase chain
reaction-restriction fragment length polymorphism (PCR-RFLP) was used for analyzing the distribution
of polymorphism at the -597C/G and -572G/A promoter of the IL-6 gene. Result: The frequency of the
196
3316 (g→a) mutation and 3394 (t→a) mutations in the diabetes group is 3.13% (7/223) and 0.9%
(2/223), respectively, and the frequency the of both the 3316 (g→a) mutation and 3394 (t→a)
mutations is 0.48% (1/223) in the healthy controls and there were no significant difference between two
groups. The other SNPs were not in both groups. Conclusions: There were no relationship between
Mitochondrial DNA 3243 (t→c), 3593 (t→c), 325l (a→g), 3264 (a→g), 3290 (t→c), 3316 (g→a), 3394
(t→c) locus mutation with Uygur patients with type 2 diabetes mellitus in Xinjiang.
A-263
More attention should be paid to mast cells/basophils than to IgE in allergy: proposed
procedure for diagnosing allergic disorders
He Shaoheng 1
1Clinical
Research Centre, the First Affiliated Hospital of Nanjing Medical University, 300 Guang-zhou Road, Nanjing, 210029,
People’s Republic of China, Tel: 0086 25 83672085; Fax: 0086 25 83672085, Email: shoahenghe@hotmail.com
For the last several decades, the routine procedure for diagnosis of immediate-type hypersensitivity
(allergy) is initially based on clinical history and physical examination; and then confirmed by skin prick
test with allergen extracts and allergen-specific IgE (sIgE ) measurement (Hamilton, 2010). This
complies with the current definition of allergic diseases：
a group of diseases largely driven via
IgE-dependent mechanisms (Barnes, 2009). However, substantial disagreement between clinical
manifestation (including skin prick tests) and sIgE measurement has been noted. For example, the
predictive value of sIgE for food allergy, drug allergy and allergic asthma is relatively poor (Hamilton,
2010), which demonstrates that we are overvalued the importance of sIgE in diagnosing allergic diseases.
According to current theory of allergy, allergens, IgE and mast cells/basophils represent the causative
factors, the transmitter (or messenger) and the effector cells of allergy, respectively. The main problem
here is that we use the transmitter IgE to define a group of diseases, which is certainly not sufficient
enough to reflect the nature of the diseases. The most important event in allergic diseases is activation
of mast cells/basophils rather than production of IgE, as they are the ones initiating the pathological
damage and causing clinical problems of allergic patients. Thus, theoretically anything which can activate
substantial number of mast cells/basophils in the body can induce allergic rections. Therefore, allergic
diseases should be defined as a group of mast cell or basophil activation provoked non-infection
diseases, including IgE mediated, IgG mediated and non-IgE or non-IgG mediated (non-immunological)
subgroups. Based upon the proposed definition above, the ideal laboratory examination procedure for
allergy should be as follows: Skin prick and skin patch tests (highly suspicious of
allergy)allergen-induced basophil activation test (confirmation of allergy) sIgE and sIgG
measurement (confirmation of subtype of allergy)final diagnosis: IgE, IgG or non-immunological
allergy.
A-264
Moving and Breathing training on short-term effect of the stable COPD patients
Li Fengsen 1*, Liao Chunyan 1, Du Lijuan 1
197
1Affiliated
TCM Hospital Xinjiang Medical University, 830000, Urumqi, China & National Clinical Research Base of Traditional
Chinese Medicine, Urumqi, 830000, Xinjiang, China; *Correspondence should be addressed to fengsen602@163.com
Objective: To observe the movement and breathing training on short-term effect of the out-patient with
stable chronic obstructive pulmonary disease (COPD) patients. Methods: 57 cases out-patient in stable
COPD patients got conventional treatment at the same time, given the movement and breathing training,
and observed parameters (FVC%, FEV1%, mMRC, the CAT, the BODE, symptom score) to evaluate the
efficacy of treatment. Results: after 6 weeks of treatment, lung function in patients with FVC%
unchanged, after 12 weeks, FVC% improved; the treatment of 6 weeks, 12 weeks in patients with
symptom score, FEV 1%, the quality of life scores (CAT) compared with the significant improvement in the
rehabilitation movement. Conclusion: Exercise and respiratory training after six weeks out-patient in
stable COPD patients with symptoms, lung function, quality of life, BODE began to improve, improved
significantly after 12 weeks of treatment.
A-265
Neuroprotective effects of icariin on corticosterone-induced apoptosis in primary
cultured rat hippocampal neurons
Liu Baojun 1, Zhang Hongying 1, Xu Changqing 1, Yang Guang 2, Tao Jiang 3, Huang Jianhua 1, Wu
Jinfeng 1, Duan Xiaohong 1, Cao Yucue 1, Dong Jingcheng 1*
1Laboratory
of Lung, Inflammation and Cancers, Huashan Hospital, Fudan University, 200040, Shanghai, China; 2Brain
Research Centre, University of British Columbia, Vancouver, V6T 2B5, British Columbia, Canada; 3Department of General
Dentistry, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology,
200011, Shanghai, China. *Corresponding author. Fax: +86 21 52888265. E-mail address: jcdong2004@hotmail.com (J. Dong).
Neurons are damaged following prolonged exposure to high concentrations of corticosterone, particularly
during chronic inflammatory and immune diseases. One of the main mechanisms underlying neuronal
injury is apoptosis. In the present study the neuroprotective effects of icariin, an active natural ingredient
from the Chinese plant Epimedium sagittatum maxim against corticosterone-induced apoptosis were
examined in primary cultured rat hippocampal neuronal cells. Pre-treatment of neuronal cells with icariin
suppressed corticosterone-induced cytotoxicity in a dose-dependent manner. Terminal deoxynucleotidyl
transferase-mediated deoxyuridine triphosphate (dUTP) nickendlabeling assay (TUNEL) labeling
demonstrated that icariin significantly reduced TUNELpositive cell numbers induced by exposure of
cultured neurons to corticosterone. Moreover, icariin markedly inhibited corticosterone-induced
mitochondrial dysfunction, including improved mitochondrial membrane potential and inhibition of
caspase-3 activation. Using western blot analysis, corticosterone activated p38MAPK, extracellular
regulated kinase 1/2 (ERK1/2) ,and c-jun N-terminal protein kinase 1(JNK1) ,while icariin blocked p38
MAPK, but not JNK1 or ERK1/2. Pharmacological approaches showed that the activation of p38MAPK
plays a critical role in corticosterone-induced mitochondrial dysfunction and apoptosis. Taken together,
the present results suggest that the protective effects of icariin on apoptosis in hippocampal neuronal cells
are potentially mediated through blockade of p38 MAPK phosphorylation.
A-266
Pathogenesis of idiopathic pulmonary fibrosis:from initial apoptosis of epithelial cells to
198
lung remodeling?
Jin Hualiang 1, Dong Jingcheng *
1Lab
of Integrative Medicine for Lung, Inflammation and Cancers, Huashan Hospital, Fudan University, Shanghai 200040, China
(Jin HL and Dong JC); *Correspondence to: Prof. DONG Jing-cheng, Lab of Integrative Medicine for Lung, Inflammation and
Cancers, Huashan Hospital, Fudan University, Shanghai 200040, China (Tel: 86-21-52888301. Fax: 86-21-52888265. Email:
jcdong2004@126.com)
Idiopathic pulmonary fibrosis (IPF) is a progressive and often fatal form of interstitial lung disease. Despite
extensive efforts in research during recent years, the mechanisms of the disease remain poorly
understood. Evidence of an inflammatory mechanism, both supportive and contrary, is briefly reviewed in
this paper. However, growing evidence has indicated that the apoptosis of alveolar epithelial cells (AECs)
may be the early driving force of progression, with subsequent disrupted integrity of the alveolar-capillary
basement membrane leading to an abnormal wound healing pathway. Thus, this paper will focus on
outlining a process of pathogenesis of IPF from initial apoptosis of AECs to end lung remodeling.
A-267
Preliminary Analysis of the miRNA Expression between Fresh and Old Peripheral
Anticoagulant Blood
Zhu Manli 1, Jiao Yi 2, Wang Ye 1, Mao Xinmin 3, Li Linlin 1*
1Department of Pharmacology,
Xinjiang Medical University, Urumqi, 830011, China; 2Department of Biochemistry and Molecular
3College of Traditional Chinese Medicinal, Xinjiang Medical University, Urumqi, 830011, China;
Biology, Basic Medical Institute；
*Correspondence should be addressed to yz-lll@vip.sina.com
To compare the expression of miRNA（
microRNA）
between fresh and old peripheral anticoagulant blood.
To choose 5 pieces of fresh blood samples and 6 pieces of old blood samples respectively，
extract total
RNA from the whole blood and measure its purity and concentration，
and analyze the microRNA
expression using real-time quantitative reverse transcription polymerase chain reaction(real-time
RT-PCR). Micro-spectrophotometer test，
A260 /280 of total RNA is 1.8~2.0. The comparative number of
copies of miRNA RNAU6-2 has no statistical differences between fresh and old peripheral anticoagulant
blood. miRNA RNAU6-expressed in fresh and old peripheral anticoagulant blood samples have no
difference.
A-268
Preliminary Analysis of Transcription Level of FOXOs Genes in Patients’ Feet and Ankles
Who Suffer Xinjiang Uygur Congenital Clubfoot
Dong Haojie 1, Lv Faming1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should
be addressed to lvgangxj@163.com
Objective: This thesis discusses Xinjiang Uygur congenital clubfoot FOXO1, FOXO3, FOXO4 genes in
ankle muscle tissue of foot and ankle conditions in order to understand their roles in development of
199
malformations of vessels thus provide a theoretical basis for the cause of Xinjiang Uighur congenital
clubfoot. Methods: To Collect 30 patients with Uygur congenital clubfoot and 30 cases healthy children.
And to collect foot and ankle muscle tissue 200mg by surgery or puncture method and venous blood 5ml.
Then to extract tissue RNA and reverse transcription kit for DNA inversion are used. By means Of
RT-PRC amplification, to research on FOXO1, FOXO3, FOXO4 changes in the level of gene transcription.
Results: Compared with the normal group, in patients’ foot and ankle muscle tissue who suffer with
congenital clubfoot, FOXO1 gene transcription levels were significantly increased (P<0.05). It has
statistical significance. FOXO3 gene transcription levels were significantly increased (P<0.05) .It has
statistical significance. FOXO4 has no significant difference in the level of gene transcription (P>0.05).
There is no statistical significance. Conclusion: The expression of transcription level of FOXO1, FOXO3
gene may be associated with the Xinjiang Uygur incidence of congenital clubfoot and FOXO1, FOXO3
gene may play an important role in the development of malformations of vessels, resulting in occurrence
of congenital clubfoot.
A-269
Prethrombotic State is a common manifestation of chronic diseases: A clinical study
T.Aji 1, Shajidan Abudoureyimu2*, Nurmuhammat Amat3, Halmurat Upur3*, R.Aizezi 3, J.Wei 3, A.Umar 4,5,
Nicholas Moore3,5
1Cardiovacular
Diseases Department,Bortala People Hospital,2First Affilated Hospital of Xinjiang Medical University, Urumqi
830054, Xinjiang Uighur Autonomous Region, PR China;3Traditional Uighur Medicine Department, Xinjiang Medical University,
Urumqi 830054, Xinjiang Uighur Autonomous Region, PR China; 4Department of Pharmacology - Xinjiang Medical University,
830054-Urumqi, Xinjiang, People’s Republic of China; 5Department of Pharmacology - Universite de Bordeaux, F-33076
Bordeaux Cedex, France; *Correspondence should be addressed to halmurat@263.net or nur818@hotmai.com
Objective: Chronic diseases including cancer, diabetes and cardiovascular diseases are thought to be
caused by the complex interplay between multiple genes and environmental factors. Studies have
indicated that a prethrombotic state, defined by the imbalance between coagulation and fibrinolysis
mechanisms, is associated with an increased tendency for individuals to develop chronic diseases. This
study aims to investigate whether a prethrombotic state is a common characteristic of chronic diseases in
humans. Methods: This study included 285 adult patients with confirmed cancer, diabetes, hypertension
and coronary heart disease. We analyzed CD62p expression in platelets, as well as plasma
concentrations of tissue plasminogen activator (t-PA), plasminogen activator inhibitor 1(PAI-1),
endothelin (ET-1), and fibrinogen (FIB). We also determined the activated partial thromboplastin time
(APTT), prothrombin time (PT) and thrombin time (TT). Results: The results of the aforementioned
indexes were compared with those obtained from a group of 30 healthy subjects who served as controls.
The results indicated that the expression of CD62P, plasma PAI-1 and FIB levels of patients were
significantly higher as compared with those obtained from healthy subjects. In cancer patients, plasma
levels of FIB and ET-1 were markedly higher compared with healthy subjects, while plasma t-PA and
APTT were markedly decreased. There were no significant differences in serum ET-1 levels and
expression of CD41 between the healthy subjects and the patient with chronic diseases. In addition, PT
was significantly shortened in patients with cancer, diabetes and hypertension compared to the healthy
subjects. There were no significant changes in APTT, PT and TT among the various disease groups.
These data indicate that there is increased platelet activation and thrombin activity but lower fibrinolytic
200
function in patients with chronic diseases, suggesting an involvement of a prethrombotic state in chronic
diseases. Conclusion: We conclude that a prethrombotic state may be an important component in the
pathogenesis of the chronic diseases.
A-270
Puerarin effect and mechanism of inhibition of airway inflammation and airway
remodeling in chronic asthma model
Zhou Linfu 1*, Wang Wenlu 1, Li Hongyan 1, Miao Weiwei 1, Wang Fengfeng 1, He Shaoheng 1, Yin
Kaisheng 1
1First
Affiliated Hospital of Nanjing Medical University, Department of Respiratory Medicine, Nanjing, 210029, China;
*Corresponding authors: Zhou LinFu, Email: lfzhou@njmu.edu.cn
Objective: To observe the puerarin on mice with chronic asthma airway inflammation and airway
remodeling, and to explore its possible mechanism. Methods: BALB/c mice 24 were randomly divided
into normal control group, asthma group, puerarin group (50 mg/kg) in the budesonide group. Ovalbumin
(OVA) sensitized and challenged 12 weeks of modeling. The last challenge, after 24 h, the determination
of airway responsiveness, HE staining and PAS staining of airway inflammation, Masson staining and
hydroxyproline determination and immunohistochemical observation of lung tissue, ELISA detection of
serum IgE and BALF cytokines level flow cytometry of spleen dendritic cells (DC) and CD4+ T cell
proliferation level, Western blot of lung tissue, thymic stromal lymphopoietin hormone (TSLP) protein
expression. Results: the asthma group airway resistance (RL) than in normal group, puerarin group and
budesonide budesonide group than asthma group significantly with lower (all P<0.05), puerarin group and
budesonide budesonide group intervention effect similar (P>0.05); asthma of BALF inflammatory cells in
the total number and eosinophilic cell ratio was significantly higher than the normal group and puerarin
group and the budesonide group than in the asthma group was significantly reduced by both (P< 0.05);
asthma group BALF cytokines (IL-4 IL -13 of TGF- β1 and VEGF) and serum total IgE, OVA- sIgE in a
more normal group was significantly higher puerarin group and the budesonide group compared with
asthmatic mice significantly reduced; Airways of the asthmatic group PAS-positive epithelial cells /
bronchus , bronchial Masson staining area / basement membrane perimeter , lung tissue hydroxyproline
content , bronchial α-SMA - positive staining area / bronchial basement membrane perimeter, lung tissue
PCNA levels than normal significantly increase the puerarin group and the budesonide group than in the
asthma group significantly reduced; asthmatic mice is higher than the normal group (P<0.05), the
treatment group compared with the asthmatic group was lower (P<0.05); asthma group splenic DC and
CD4+ T cell proliferation level than the normal group was significantly higher puerarin group and the
budesonide group than in the asthma group were significantly decreased (P<0.05); TSLP expression of
asthmatic lung tissue was significantly higher than the normal group and puerarin group and the
budesonide group than in the asthma group were significantly (P<0.05). Conclusion: Puerarin can inhibit
the chronic asthma model, airway inflammation, airway hyper responsiveness and airway remodeling;
this inhibition may be related to inhibition of TSLP- DC -mediated Th2 immune.
A-271
Regulation of STAT signaling in mouse bone marrow derived dendritic cells by
respiratory syncytial virus
201
Jie Zhijun 1,2, Darrell Dinwiddie 2, Albert Senft 2, S.H kevin 2*
1 Department
2
of Respiratory Medicine, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai, 200240, China;
Infectious Disease Program, Lovelace Respiratory Research Institute, Albuquerque, NM 87108, USA.; * Corresponding author
at: Infectious Disease Program, Lovelace Respiratory Research Institute, 2425 Ridgecrest Drive, SE, Albuquerque, NM 87108,
Tel: 1-505-348-9488; Fax: 1-505-348-8567.
Email: kharrod@lrri.org; Email addresses:
jiezjlxh@gmail.com (Z. Jie),
ddinwiddie@lrri.org(D. Dinwiddie), asenft@lrri.org (A. Senft), kharrod@lrri.org (K. Harrod)
Objective: Dendritic cells (DCs) act as a portal for virus invasion as well as potent antigen-presenting
cells (APCs) involved in the antiviral host response. Interferons (IFNs) are produced in response to
bacterial and viral infection and activate innate immune to efficiently counteract and remove pathogenic
invaders. Respiratory syncytial virus (RSV) could inhibit IFN-mediated signaling pathway in epithelial
cells; however, the effects of RSV on IFN signaling in the dendritic cells (DCs) are still unknown.
Methods: Mouse bone marrow derived DCs (BMDCs) were mock or infected with RSV at different
multiplicity of infection (MOI) for 24h, and then treatment with different cytokines such as interferon-β
(IFN-β), IFN-γ or interleukin-10 (IL-10). The RNA expressions of RSV nonstructural protein-1 (NS-1) and
NS-2 were detected by RT-PCR. The expressions of Janus family kinase-signal transducer and activator
of transcription (JAK/STAT) signaling proteins were assessed by immunoblotting assays. The nuclear
localization of specific signaling proteins was determined by immunoflourescence assay. Results:
Increasing amounts of NS-1 or NS-2 mRNA expression in BMDCs were observed with infected RSV at
increasing MOI, suggesting BMDCs were permissive for viral gene expression. Further examination of the
IFN-β signaling cascade showed RSV infection increased the total cellular levels of STAT1 and STAT2 in
BMDCs, but impaired the IFN-β-dependent phosphorylation and nuclear localization of STAT1 and
STAT2. The inhibitory effects of RSV on STAT1 and STAT2 phosphorylation and translocation were
abolished by ultraviolet inactivation. In contrast, RSV did not inhibit the IFN-γ-stimulated STAT1
phosphorylation and nuclear localization. IL-10-stimulated STAT3 phosphorylation was also unaffected by
RSV. Conclusions: Despite RSV inhibit STAT protein levels through degradation mechanisms in
epithelial cells, these findings demonstrate that RSV also can specifically inhibit the type I interferon
response in BMDCs through regulation of STAT1 and STAT2 phosphorylation and nuclear translocation.
A-272
Research on expression regulation of relevant cytokines of Th17 in COPD Mouse lung
tissue
，
Wang Jing 1，
Li Fengsen1 2*，
B.Aishan 1
1
2Xinjiang State Research Center of Traditional
Affilated Traditional Chinese Medicine Hospital of Xinjiang Medical University；
Chinese Medicine, Urumqi, 830000, Xinjiang Uighur Autonomous Region，China
Objective: Research changes of the chronic obstructive pulmonary disease (COPD) mouse Th17 cell.
And preliminary study the relationship between Th17 cells with COPD pathogenesis. Methods: The
mouse model of COPD was established through the application of smoke continued for 6 months, to
collect lung tissue and peripheral blood, measured by ELISA in serum IL-6, IL-17 and IL-10 changes in the
level, using Real-Time PCR to detect lung tissue STAT-3 of IL-17 and RORγt mRNA changes in levels.
Results: The COPD model group serum IL-17 and IL-6 content was significantly higher than the normal
control group (P <0.05), while the expression of IL-10 model group for the control group was significantly
202
higher (P <0.05) ; with COPD mouse lung tissue STAT-3, IL-17 and RORγt mRNA level was obviously
higher than the control group(P <0.05). Conclusion: Th17 cell may play an important role in the
pathogenesis of COPD. And it may induce the development of pulmonary inflammation.
A-273
Seeking best menu of walking exercise for a healthy volunteers through the regulation of
peripheral white blood cells in number
Nobuo Yamaguchi1, Delixat Yimit 2, Nurmuhammat Amat 2, Parida Wuxur 2, Xue Jie2
1Kanazawa
Medical University, Kanazawa, Japan; 2 Traditional Uighur Medicine Department of Xinjiang Medical University, 393
Medical University Road, 830011, PR China
Efficacy and impact of walking exercise has been widely recognized. However, the majority of walkers did
not have a scientific background to know the best exercise menu for the one’ QOL; quality of life. The
purpose of this study was to demonstrate the best menu of walking that regulates the peripheral white
blood cells in number and function as a marker of QOL expression. Healthy male and female subjects
who gave informed consent (mean: 41, n=10) were used. Each subject received two types of exercise
treatments at intervals of one month or more. The volunteer direct to walk within one hour for 4 km,
indicating 4 mets of exercise. On the other hand, the same volunteer asked to clear within the 30 min for
the same course; 8km/he, 8mets. The blood samples were collected before and 24 hrs after the exercise,
analyzing by conventional procedures and measured the total leukocytes number, ratio of granulocytes
and lymphocytes. The adrenalin contents were also measured. Relative changes in total cell counts,
ratios of lymphocytes and granulocytes different in the two groups. The regression lines also represented
different tendencies. These results showed that 4 mets walking showed the strong regulative effect
-0.6893 but 8met walking -0.3618 of lymphocyte, far behind the 4met therapy in CAM as an individualized
therapy.
A-274
Significance and Enlightment of the Discovery on the Blood, Circulation Driven by
Respiration and of the Study on the Mechanism of the Day-Sweat and the Night-Sweat
Li Zhigang1
1
Yu Lin Middile Agricultural School, 71900, Yulin Shaanxi, China
The significance of the discovery on the blood’s circulation driven by respiration is that by the discovery
people can know how the respiration effects on the function of the heart and the blood vessel, furthermore
can guide their clinical practices better. The significance of the study is that the cause of the mechanism
of the day-sweat and the night-sweat is still unknown, people can explore the mechanism of the
day-sweat and the night-sweat, thus to know how the day-sweat and the night-sweat effect on human
body and what remedy is the most effective for them. The enlightment of the discovery and the study is by
them people can know that the scientifical experiment is not the only method for finding out something.
A-275
The apigenin raised CD4 + CD25 + Treg cells and inhibit the study of the role of airway
203
inflammation in acute asthma model
Zhou Linfu 1*, Li Hongyan 1, Wang Wenlu 1, Wang Fengfeng1, Miao Weiwei 1, He Shaoheng1, Yin
kaisheng 1
1First
Affiliated Hospital of Nanjing Medical University, Department of Respiratory Medicine, Nanjing, 210029, China;
*Corresponding authors: Zhou LinFu, Email: lfzhou@njmu.edu.cn
Objective: To observe the celery factors for acute asthma mouse model of airway inflammation, airway
hyper responsiveness and CD4+CD25+ regulatory T cells (Treg) affect. Methods: 30 SPF grade female
BALB/c mice were randomly divided into control group, asthma group, removal of CD25 apigenin group,
apigenin group (20mg/kg) and the budesonide group. Ovalbumin (OVA) sensitization and excitation
modeling. The last challenge after 24 h, the determination of airway responsiveness , HE staining and
PAS staining of airway inflammation and mucus secretion , ELISA assay of serum IgE and BALF
cytokines by flow cytometry spleen Treg level. Results: the asthma group airway resistance (RL) than in
normal group , celery hormone group and the budesonide budesonide group RL than the asthma group
significantly with lower , removal of CD25 celery hormone group than the celery prime group significantly
with the increase (both P<0.05) ; asthma of BALF inflammatory cells in the total number and eosinophilic
cell ratio was significantly higher than the normal group , apigenin group and the budesonide group than
asthma group was significantly lower , remove a significant increase in CD25 of apigenin group compared
with the apigenin group (all P <0.05). asthma group in BALF of Th2 (IL-4 , IL-13), serum total IgE and
OVA-sIgE were significantly higher than the normal group , the apigenin group , the budesonide group
than in the asthma group were significantly lower , removal of CD25 apigenin group compared with the
celery factors were significantly higher (P<0.05); Asthma group in BALF of Th1 (IFN-γ) cytokines than the
normal group was significantly lower , celery hormone group , budesonide budesonide group compared
with asthma group increased significantly , removal of CD25 celery prime group celery prime group
significantly with lower (all P<0.05) ; asthma group spleen Treg levels than in normal group was
significantly reduced apigenin group, spleen Treg levels in the budesonide group compared with the
asthma group were significantly increased, removal of CD25 apigenin group than in the apigenin group
decreased significantly (P<0.05). Conclusion: Apigenin can inhibit acute experimental asthma, airway
inflammation and airway hyperresponsiveness, this inhibition may increase Treg levels and Th2 immune
response into immunosuppression.
A-276
The influence of different electroacupuncture strength between inflammatory cells factor
and cerebral edema in cerebral ischemia reperfusion rats
Duan Lijun 1, Li Heping 1
1
The First Affilated Hospital, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region，China
Objective: To study the effects of inflammatory cytokines and brain edema in rats of acute cerebral
ischemia and reperfusion with different electroacupuncture stimulation intensity. Neurological deficit
score (NSS) and the infarct area were observed in each group. The levels of inflammatory cytokines,
TNF-α, IL-1β, IL-10 and the content of tissue water in brain in the peripheral blood serum were analysed.
Explore the therapeutic effect of acupuncture strength of the best EA on rats of cerebral ischemia and
reperfusion. Methods: 8 week-old SD rats were randomly divided into 3 groups: the normal group, 10; the
204
sham operation group, 10; model group, 30. Established the middle cerebral artery occlusion (MCAO)
and reperfusion model in the model group, then divided its into three groups: the model control group, the
weak EA group, the strong electro-acupuncture group. Did the acupuncture 6 hours after the
ischemia-reperfusion (I/R). Did the NSS, collected blood of abdominal aortic and the brains after 24 hours.
The brain of 2 rats in every intervention group were frozened section, and the infarct area was observed
with TTC staining. Meanwhile, the brain water content were detect with wet and dry method. detected the
levels of TNF-alpha, IL-1β, IL-10 in the Serum, and the statistical analysis was done for every index.
Results: 1. varying degrees nerve function deficit and the right eye Horner's certificate were dicovered in
the model group, and the normal group and sham group does not appear above signs, and varying
degrees of cerebral infarction were discovered after the TTC staining; 2. Compared with the normal
group, the levels of inflammatory cytokines, TNF-α, IL-1β, IL-10 and the content of tissue water in brain
were no statistically significant differences (P＞
0.05) in the sham operation group, and its increase
significantly (P＜
0.01, P＜
0.05) in the model control group; 3. Compared with the model group, the levels of
TNF-alpha, IL-1β decreased significantly (P＜
0.05, P＜
0.01), the levels of IL-10 and the content of tissue
water in brain were no statistically significant differences (P＞
0.05) in the weak EA group. But, in the strong
EA group, the levels of TNF-alpha, IL-1β and the content of tissue water in brain decreased significantly
(P＜
0.01, P＜
0.05), while, the levels of IL-10 increased significantly (P＜
0.01); 4. Compared with the weak EA
group, the levels of TNF-alpha, IL-1β decreased significantly (P＜
0.05), the levels of IL-10 and the content
of tissue water in brain were no statistically significant differences (P＞
0.05). Conclusion: EA can protect
the rats in the acute cerebral ischemia reperfusion by reducing TNF-alpha, IL-1β and brain tissue water
content and increasing IL-10 levels of the serum. The efficacy are difference among the different intensity
EA, strong stimulation is superior to the weak stimulation.
A-277
The Research of Asthma patients with exhaled nitric oxide and blood eosinophil levels
，
Yang Weijiang 1, Wang Nana 1, Li Fengsen 1 2*
1
2Xinjiang State Research Center of Traditional
Affilated Traditional Chinese Medicine Hospital of Xinjiang Medical University；
Chinese Medicine, Urumqi, 830000, Xinjiang Uighur Autonomous Region，China
Objective: to investigate patients with asthma between the exhaled gas of nitric oxide and blood
eosinophil percentage level. Methods: 160 cases of patients with acute asthma detect exhaled gas nitric
oxide and eosinophil percentage of cell content were collected to study the correlation. Results: patients
with acute asthma between the exhaled gas of nitric oxide and blood eosinophil percentage were
positively correlated. Conclusion: patients with asthma detection of exhaled nitric oxide and blood
eosinophil percentage can improve the diagnostic accuracy.
A-278
The Study of IL-4、
IFN-γ Gene Methylation of Allergic Rhinitis in Uygur, Han people
Lou Zhongzhang 1, Gao Li 1*
205
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should
be addressed to Xjmugaoli@sina.com
Objective: To investigate the difference of IL-4, IFN-γ gene promoter methylation of allergic rhinitis
patients and normal control in Uygur, Han people. Methods: Methylation-specific PCR (MSP) detected
IL-4, IFN-γ gene methylation of 50 Uygur cases and 50 Han cases with allergic rhinitis, and 50 normal
Uighurs and 50 normal Han cases. Results: The comparison of IL-4 gene methylation rate between the
Han nationality AR patients with normal control group is statistically significant (P<0.05). The comparison
of IL-4 gene methylation rate between the Han and Uygur in normal control group is statistically significant
(P<0.05). The comparison of IL-4 gene methylation rate between the Han and Uygur AR patients is not
statistically significant (P>0.05). The comparison of IL-4 gene methylation rate between Uygur AR
patients and normal control group is not statistically significant (P>0.05). The comparison of IFN-γ gene
methylation rate between the Han and Uygur AR patients, between patients with AR and normal controls
have no statistical significance (P>0.05). But the comparison of IFN-γ gene methylation rate between the
Han nationality AR patients with normal control group is statistically significant (P<0.05). Conclusion:
Artemisia and pigweed are two important aeroallergens of Uygur and Han patients with allergic rhinitis.
There is no significant difference in expression of IL-4 gene methylation between the Han and Uygur AR
patients. The IL-4 gene methylation rate of the Han patient with AR is higher than the normal Han person,
which indicates that IL-4 gene methylation may play an important role in the pathogenesis of allergic
rhinitis. The IL-4 gene hemimethylated of the Han nationality group is higher than that of the Uygur
nationality group. The IL-4 gene methylation in the Han nationality group may tend to be the positive
expression, the Han population susceptible to allergic rhinitis. There is no significant difference in
expression of IFN-γ gene methylation between the Han and Uygur AR patients. The role of IFN-γ gene
low-methylation in allergic rhinitis needs further research.
A-279
The Study on the relationship between Cruor-fibrinolysis system and the HuiZhuoTanZu
Syndrome of Coronary Heart Disease
Xie Xiaoliu1, An Dongqing1*
1Traditional
Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to andongqing3@gmail.com
Objective: The aim of this study is to investigate the plasma level of Fibrinopeptide A (FPA) and
Thrombin Activatable Fibrinolysis Inhibitor (TAFI) so as to explore the relationship between CHD Hui
ZhuoTanzhu Syndrome and endothelium dysfunction, provide the microcosmic objective basis for
working out the diagnose standard for the coronary heart disease Hui ZhuoTanZu Syndrome, in order to
make its diagnose tend being externalization. Methods: A total of 170 samples were obtained from the
Medical University, including 88 cases of CHD huizhuotanzu syndrome and 82 cases of CHD
non-huizhuotanzu syndrome, 80 cases of normal controls, then observe and analyses the general data
and the plasma level of FPA､TAFI among three groups. Results:1) Comparison of plasma
FPA:Compared with normal controls, the lever of FPA of CHD HuiZhuoTanzhu Syndrome group and nonHuiZhuoTanzhu Syndrome group are significantly higher, the difference is statistically significant (P<0.01)
;between CHD HuiZhuoTanzhu Syndrome group and the non- HuiZhuoTanzhu Syndrome group, there is
a significant difference in the lever of FPA (P<0.05). 2) Plasma TAFI level in comparison:the lever of TAFI
206
in CHD HuiZhuoTanzhu Syndrome group and non-HuiZhuoTanzhu Syndrome group are higher than
healthy control group, the difference is significant (P<0.01), and the lever of TAFI in CHD HuiZhuoTanzhu
Syndrome group is higher than non-HuiZhuoTanzhu Syndrome group (P<0. 05). Conclusion: The
Pathological basis of CHD HuiZhuoTanZu syndrome is related with the blood high pour-point state, there
are more anomalies in Blood coagulation-fibrinolysis system function between the CHD HuiZhuoTanZu
syndrome and non-HuiZhuoTanZu syndrome. The increasing contents of FPA､TAFI can help us to
differentiate the CHD HuiZhuoTanZu syndrome and non-HuiZhuoTanZu syndrome.
A-280
Unique performance like pleural effusion fatal huge mediastinal liposarcoma a case
Zhou Linfu 1*, Li Meimei1, Zhang Shijiang2, Shao Yongfeng 2, Huang Mao 1, He Shaoheng 1, Yin
Kaisheng 1
1First
Affiliated Hospital of Nanjing Medical University, Department of Respiratory Medicine, Nanjing, 210029, China; 2First
Affiliated Hospital of Nanjing Medical Universit, Cardiothoracic Surgery, Nanjing, 210029, China; *Corresponding authors: Zhou
LinFu, Email: lfzhou@njmu.edu.cn
A female patient, aged 38, farmer, was admitted to hospital because of progressive dyspnea February.
Occasional dry cough, fever, night sweats, chest pain, haemoptysis, paroxysmal nocturnal dyspnea.
Chest radiography and chest CT were outside the hospital reports right pleural effusion (CT value of Hu).
The thoracentesis anchor outside the hospital is very low, only see 110 mL reddish pleural effusion,
pleural effusion biochemicals show exudate. A history of schizophrenia, a history of ectopic pregnancy
surgery. Physical examination: T: 36.5 ℃
, P: 86 times / min, and R: 18 times / min, and BP: 110 /70 mmHg
in Clear consciousness, spirit wilt, orthopnea, and lips cyanosis, tracheal left superficial lymph nodes not
palpable swelling. Full right side of the thorax, fremitus enhanced percussion real tone, the right lung
breath sounds disappear. The left lung breath sounds were significantly reduced no smell and wet and dry
rales. Heart, abdomen, limbs, and nervous system examination was normal. Supplementary examination:
admission check ESR 34 mm / h; C -reactive protein 14.1 mg / L ; blood gas analysis (FiO2 33%): at rest
, of pH 7.43 , PCO2 of 41 mmHg , PO2 70 mmHg in the BE 2.6 mmol / L, SaO2, 94 %; blood, brain
natriuretic peptide , liver and kidney function , tumor markers , tuberculosis , rheumatism , autoimmune
indicators are normal. Abdominal B ultrasound was normal. Enhanced chest CT scan showed a right
pleural liquid density seen a homogeneous mass (CT value of about 25 to 67 Hu), were seen in lipid
density (CT value -94 Hu), the right lung is not, mediastinum to the left. Chest MRI showed right pleural
space-occupying completely, clear boundary, signals uneven strips separated the right side of a small
amount of pleural effusion. Urgent consultation of the hospital, and immediately transferred to the
Department of Cardiothoracic Surgery undergoing open resection of the tumor. Surgery Chengshun Li,
take out a whole gigantic mass, brittle, size 35 cm x 26 cm in x 12 cm, weighing 4.5 kg. After HE staining
and immunohistochemistry show well-differentiated and mucinous mediastinal liposarcoma. After two
weeks in patients discharged, follow-up so far no recurrence was found. Phosphatidylinositol kinase
catalytic subunit (PIK3CA) gene mutations and survival in patients with a decline. Surgical specimens of
gene sequencing and TA cloning. No. 10 of the PIK3CA gene in patients with exon 4 mutations, including
the 1615, 1627 and 1634 base point mutation, and 1658 bp frameshift mutation. Kinase spectroscopy
confirmed that the NF - κB and Src mucinous liposarcoma is the more active of the two signaling
pathways. Combined with gene sequencing and the kinase spectral data, and will undoubtedly promote
207
effective clinical conversion. Clinical intrathoracic liposarcoma is rare, but we have to think about the
possibility of a mediastinal liposarcoma in the differential diagnosis of pleural effusion.
A-281
Winter Disease consumer Clinical evaluation of asthma paste the treatment of chronic
obstructive pulmonary disease
Wu Ruohan1, Bian Yongjun1, Li Guangxi1, Li Hui1, Wang Lei1, Liu Zhiguo1, Liu Shigang1, Li Junling1, Liu
Li1, li Guoqin1
1Guang’anmen
Hospital affiliated to China Academy of Chinese Medical Sciences, 100053, Beijing, China
Objective: To verify the clinical efficacy of Xiaochuangao Acupoint Paste (XAP) to treat chronic
obstructive pulmonary disease (COPD) in the stable phase. Method: A multi center, randomized,
double-blind, placebo control study was adopted.142 patients were randomly divided into two groups.
Each year the treatment group were treated with XAP while the other group were treated with placebo
three times. The the lung function，
SGRQ life quality score，
BODE index，
yearly average times of common
cold and acute attack，
times of emergency clinics and hospitalization, and so on were observed. Result:
Except the total part of SGRQ score, other three parts are lower than the same time in control parts
(p<0.05). The same outcome happened in BODE index, the yearly average times of common cold and
acute attack (p<0.05). Conclusion: The XAP can significant alleviate the clinic symptoms, improve the
quality of life and improve the prognosis of COPD.
A-282
Xinjiang Uygur congenital clubfoot genepolymorphism analysis
Dong Haojie 1, Lv Faming 1*
1
Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，
Huanghe Road, Urumqi 830011, Xinjiang
Uygur Autonomous Region, China; *Correspondence should be addressed to lvgangxj@163.com
Objective: Of Xinjiang Uygur congenital clubfoot FoxO1, FoxO3, FoxO4 genes in ankle muscle tissue of
foot and ankle conditions，
to understand its role in the development of vascular malformations for Xinjiang
Uygur congenital clubfoot and the cause of the disease and provide a theoretical basis. Methods: We
collected Uygur congenital clubfoot in 30 patients, healthy children (including Uighur and Han)30 cases,
and collected muscle tissue 200mg and blood 5mlby the foot and ankle surgery or puncture method,
extraction of tissue RNA, reverse transcription kit for DNA inversion, amplified by RT-PCR of FoxO1,
FoxO3, FoxO4 changes in the level of gene transcription study. Results: Compared with the normal
group，
patients with congenital clubfoot foot and ankle muscle tissue FoxO1 gene transcription levels were
significantly increased (p<0.05), there existed significant difference，
FoxO3 gene transcription levels were
significantly increased (p<0.05), there existed significant difference，
FoxO4 had no significant difference
in the level of gene transcription (p>0.05) and was no statistically significant. Conclusion: FoxO1, FoxO3
gene upregulation may be related to the Xinjiang Uygur incidence of congenital clubfoot associated，
FoxO1, FoxO3 gene in the foot and ankle vascular malformations may play an important role in the
development, resulting in a congenital clubfoot occurrence.
208
A-283
Original article accuracy of 18F-FDG PET/CT for lymph node staging in non-small-cell
lung cancers
Liu Baojun1, Dong Jingcheng1*, Xu Changqing1, Z.Chuantao 1, L.JingJing 1, G.Yihui 1, Z.Jun 1, Wu
Jinfeng1, Duan Xiaohong1, Cao Yuxue1
1Laboratory
of Lung, Inflammation and Cancers, Huashan Hospital, Fudan University, 200040, Shanghai, China; *To whom
correspondence should be addressed (Full postal address :Laboratory of Lung, Inflammation and Cancers, Huashan Hospital,
Fudan
University,
Shanghai,
China
200040.Telephone
+86-21-52888301;
Fax
+86-21-52888265;
E-mail:
jcdong2004@hotmail.com)
Background: This retrospective study evaluated the diagnostic accuracy of 2-(F18)-fluoro-2-deoxy-Dglucose-positron emission tomography (18F-FDG-PET)/computed tomography (PET/CT) in the
preoperative diagnosis of metastatic mediastinal and hilar lymph node in patients with non-small-cell lung
cancer (NSCLC). Methods: A total of 39 patients received preoperative 18F-FDG PET/CT and the
postoperative biopsy. We compared preoperative PET/CT scan results with corresponding intraoperative
histopathalogic findings in 39 NSCLC patients. The sensitivity, specificity, accuracy, positive and negative
predictive value of 18F-FDG PET/CT were assessed. Results: Histopathologic examination confirmed
metastasis in 57 out of the 208 excised lymph nodes; 23 of the 57 nodes were mediastinal and hilar lymph
nodes. The sensitivity, specificity, accuracy, positive predictive value and negative predictive value of
PET/CT in the preoperative diagnosis of mediastinal lymph node metastasis in NSCLC patients were
65%, 96.8%, 92%, 78.5% and 90%, respectively. Conclusions: PET/CT scan showed good accuracy in
the preoperative diagnosis of mediastinal and hilar lymph node metastasis in the patients with NSCLC.
We recommend that PET/CT scanning be used as a first-line evaluation tool for tumor diagnosis, therapy
evaluation and follow-up.
A-284
A new trial for relief-oriented design of molecule from natural ginseng other than hot
water extraction
209
Nobuo YAMAGUCHI1, Tsugia MURAYAMA2, Masafumi NAKATO3, Yasuhiro KOMATSU4
1Kanazawa
4INMPRC,
Medical University, Kanazawa, Japan; 2Hokuriku University, Kanazawa, Japan; 3INMPRC, Kanazawa, Japan;
Tokyo, Japan
Almost all the Traditional Chinese Medicines (TCM) from plant origin are prepared by hot-water
extraction. This type of extraction system had been succeeded more than twenty centennials. However
hot water extraction remained almost all the material left as remnant. So it is necessary to recruit a whole
materials as effective compound at this modern century．
In such a circumstances, we tried to depredate
the plant material in order to prepare more neat element form ginseng. We tried to degradate the natural
material into piece by mechanically, enzyme mediated and microbial degradation. In order to screening a
effective molecule, we set up fundamental and clinical screening system. The first toxic test was
nominated by Helsinki declaration for mammals, indicating genetic toxicity for microorganism and mice.
The new effects from the materials coming out the enhanced Immmunomodulatory effect, Decrease
blood pressure, Anti-diabetic effect, Enhancing sexual potency, Relieve fatigue, Reduce cholesterol
levels, formation of the transformed ginsenosides, (Rh2, Rh3 etc.) , easy absorption from the
gastro-intestinal tract, production of new metabolites of ginsenosides.
A-285
Antibacterial Activities of Extracts and Constituents in the Rhizomes of Diploid and
Tetraploid Gingers (Zingiber officinale Roscoe)
A.Patar 1, N.Tanaka 2, H.Maeda 1, K.Ishimaru 2, K.Nishikawa 1, 3, N.Amat *
1Faculty
2 Faculty of Agriculture,
of Health and Living Sciences Education, Naruto University of Education,Tokushima, Japan；
3 Traditional Uighur Medicine Department, Xinjiang Medical University, 393 Medical University
Saga University, Saga, Japan；
Road, Urumqi 830011, Xinjiang, PR China；
*Corresponding author. Prof Nurmuhammat Amat. Traditional Uighur Medicine
Department, Xinjiang Medical University, 393 Medical University Road, Urumqi 830011, Xinjiang, PR China Tel.: +86 991
4366167; fax: +86 991 4366014 E-mail address: nur818@hotmail.com
The pungent phenolics were also isolated from the rhizome of tetraploid ginger (Zingiber officinale
Roscoe), and their structures were established as [6]-gingerol and [6]-dehydroparadol by chemical and
spectroscopic evidence. And extracts and constituents in the rhizomes of diploid and tetraploid gingers
were screened for their antibacterial activity against 6 Gram-negative bacteria (Escherichia coli,
Pseudomonas aeruginosa, Salmonella typhimurium, Salmonella enteritidis, Vibrio parahaemolyticus,
Yersinia enterocolitica) and 3 Gram-positive bacteria (Staphylococcus aureus, Listeria monocytogenes,
Bacillus cereus). The minimum inhibitory concentrations (MICs) of extracts and constituents in the
rhizomes were determined by the microdilution method ranged from 0.49 to 16,000g/ml, and 0.03 to
1,000g/ml, respectively. Extracts and constituents in the rhizomes exhibited a fairly high antibacterial
activity against the spectrum of all Gram-positive bacteria tested.
A-286
Biodiversity: A link between traditional medicine and modern medicine
Anake KIJJOA1
210
1Instituto de Ciências Biomédicas de
Abel Salazar and Centro Interdisciplinar de Investigação Marinha e Ambiental
(CIIMAR),Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal
Thai traditional medicines have always been popular as traditional household medications and a long
tradition of preparing and using herbs both as food and as medicine is still being practiced in the Thai
society. Recently, Thailand has adopted a policy to support Thai Traditional Medicine as an effective and
cheaper way to promote good health of the population. Even though a small number of plant species are
used as medicinal plants in herbal preparations, many have been popularly claimed to have properties in
curing variable diseases. With a vast area of tropical forest and coast lines, Thailand has inexhaustible
biodiversity waiting to be exploited. For these reasons, our group has been investigating chemical
constituents and their biological activities of the species popularly claimed as having medicinal properties
and the related species. Bearing in mind that the tropical soils are also rich of microbial species, we have
also investigated the bioactive compounds produced by soil fungi which exhibited synergistic or
antagonistic action with plant pathogens. Although there is no record of medicinal uses of marine
organisms, the seas of Thailand are considered potential reservoirs of biodiversity and genetic resources.
Consequently, we have also investigated the compounds produced by the marine sponges and the
sponge-associated fungi which led to isolation and identification of several new compounds with
interesting skeletons and biological activities. The bioactive compounds isolated from either plant with
traditional use or unclaimed species are potential for further developments of new therapeutic arsenal to
combat rare and newly emerging diseases.
A-287
Characteristics of traditional acupuncture therapies in Japan, Korea and China
Kenji Kawakita1, Yong-Suk Kim2, L.XiaoPin 3, Matsuo Arai4, Naomi Takazawa5, Nobuo YAMAGUCHI6
1Meiji
University of Integrative Medicine, Kyoto Japan; 2Kyung-Hee University, Seoul, Korea; 3Liaoning University of Traditional
Chinese Medicine, Liaoning, China; 4INMPRC, Kanazawa, Japan; 5Takazawa Acupuncture Clinic, Tokyo, Japan; 6Kanazawa
Medical University, Kanazawa, Japan
Efficacy and safety of acupuncture therapies has been widely recognized. The majority of acupuncture
practitioners have learned acupuncture in China (C). On the other hand, different acupuncture procedures
have also developed in Korea (K) and Japan (J). The purpose of this study was to demonstrate the
characteristics of acupuncture procedures performed in three countries. Healthy male subjects who gave
informed consent (mean: 21, n=10) were used. Each subject received three treatments at intervals of 2
months or more. The practitioners were asked to make diagnosis and selected best points as
individualized therapy for improving health conditions. The blood samples were collected and analyzed by
conventional procedures and measured the total leukocytes number, ratio of granulocytes and
lymphocytes. The adrenalin contents were also measured. Total number of points used in C and K were
similar but they were obviously different. In J, shallow acupuncture needling along the channels was done
and no name of points was recorded. Relative changes in total cell counts, ratios of lymphocytes and
granulocytes differed among the J, C and K. The regression lines also represented different tendencies.
These results were not conclusive but we found variation of acupuncture treatments in J, K and C. They
have potentials to modulate the immune systems and stress levels in different way. This JCK projects was
the first attempt and it offered important information to establish the role of acupuncture therapy in CAM
211
as an individualized therapy.
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Current Trends in Medical Education
Muhammad Aslam1
1Shifa
College of Medicine, Shifa Tameer-e-Millat University, Sector H – 8/4, Islamabad
A future medical expert [doctor] needs to acquire appropriate scientific foundation [knowledge] for which
one has to develop critical thinking and communication; needs to learn clinical skills in order to be
professionally competent; needs to develop professional attitude and values for which one has to improve
behaviour and collaborations and, needs to be a custodian of population health as a social responsibility
for which one has to learn managerial skills and health advocacy. In order to achieve, it is need of the hour
to transform teaching to learning, memorizing to understanding and critical reasoning, and examinations
to evaluations. We need not to follow criteria but to set standards. The curriculum is required to be
student‐centered, patientoriented, integrated, community based, elective driven and systemic. The
tomorrow’s curriculum is required to take the pillars of Bloom’s taxonomy into an account [knowledge,
comprehension, application, analysis, synthesis and evaluative judgment]. We need to hybridize the
component of research in all facets. The content overload and redundancy is required to be shredded and
concepts and context [relevance] needs to be merged. Multimodal learning strategies like large group
interactive sessions, small group discussion, self‐directed learning, computer‐assisted learning,
problem‐based learning, casebased learning, patient‐based learning, performance‐based learning and
projects/ assignments are to be introduced and encouraged. The evaluation methodology must be
specific, measurable, achievable, realistic and timebound. Efforts be made to transform summative
assessment to formative assessment. The formative assessment reforms the process of learning,
whereas, summative assessment reframes the outcome. In conclusion, we need to keep abreast with the
current trends in curriculum development, learning strategies and accreditation system keeping an
account of our regional and cultural delicacies.
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Education Management in Faculty of Medicine in Kabul Medical University, Kabul,
Afghanistan
Samad Omar1
1
Dean, Faculty of Medicine Kabul Medical University
Faculty of Medicine founded in 1932 and is the first higher education institute in the country, throughout
years of progress it is promoted to Kabul Medical University which includes 5faculties. The education
management is according to law and regulation of Ministry of Higher Education. Selection of students is
through an entrance exam. Study in Faculty of Medicine takes 7 years; MD degree is given to the
graduates. Each university year is 2 semester and each semester is 16 weeks. Lectures, practical work in
laboratory, bedside teaching, PBL, CBL are methods which are used for teaching. Evaluation of students
is carried out during the semester and at the end of each semester.
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A-290
Effectiveness of Integrated Medical Curriculum in Pakistan: A Public Medical College
Experience
Muhammad Saeed1*, Muhammad Iqbal Khan2, Zeiyad Afzal Keyani3, Sarmad Lateef Awan4, Syed Sajid
Hussain Shah5, Mohsin Shakeel6
1Prof.
of Anatomy & Associate Dean; AJK Medical College, Muzaffarabad, Pakistan; 2Prof. of surgery & Dean; AJK Medical
College, Muzaffarabad, Pakistan; 3Assistant Dean Medical Education；AJKMC, Muzaffarabad, Pakistan; 4Assistant Prof. of
Anatomy, AJKMC, Muzaffarabad, Pakistan; 5Prof. of Pathology, AJKMC, Muzaffarabad, Pakistan; 6Assistant Prof. of Urology,
AJKMC, Muzaffarabad, Pakistan. *Correspondence: Dr. Muhammad Saeed Shafi, Prof. of Anatomy & Associate Dean AJK
Medical College, Muzaffarabad, Pakistan; E-mail: msaeedshafi@hotmail.com; Fax # +92-5822-920523; Ph. +92-5822-920448;
Cell: +92-333-4603013.
Background: Integration of medical curricula is still a growing concept in Pakistan. Rapidly changing
healthcare system (Healthcare teams) and patient centered care has compelled Medical Regulatory Bodies for
a paradigm shift in medical curricula1-2. Keeping abreast with the global trends and watching changed local
needs, Azad Jammu & Kashmir Medical College (AJKMC) was the 1st public sector medical college in
Pakistan to adopt an outcome based integrated undergraduate curriculum since its inception in 20113. To
contextualize the training of future physicians and make Basic Health Sciences (BHS) instruction more
relevant to the clinical practice; we introduced integrated, system based Modular spiral curriculum at
trans-disciplinary level. Study Design: An action research involving mix-method approach with a dominant
qualitative focus. Action research is a participatory research which involves the researchers and participants in
an iterative loop of analysis and planning an intervention; evaluation of its impact; and implementation of the
solution found in next cycle of research4. Summary of work: Five modules of pre-clinical phase (Spiral-1) of
AJKMC Curriculum have been delivered to the class of 2016 till Aug. 2012. The first spiral of undergraduate
medical curriculum deals with foundation of medicine (normal structure & function) along with introduction to
professionalism, contemporary biomedical ethics, history of medicine, culture, diversity, sanctity of human
body/tissue, information & communication technology, learning styles & approaches, small group dynamics,
theories of learning, assessments, reflective writing, and legacy of Muslim physicians. The learning outcomes,
curriculum map, teaching and learning strategies and blue print for assessment was provided to faculty and
students in Study Guide of each module. All the modules delivered so far were evaluated for their effectiveness
and impact on students learning at the end of each module by a semi-structured questionnaire on a 5-point
likert scale, having open ended comments column also5. Summary of results: Triangulation of quantitative &
qualitative data revealed positive perceptions of students about integrated curriculum. The response rate of
voluntary participants was 92%. Ninety six % participants graded all sessions as good/excellent/outstanding;
whereas 1% poor and 3% fair. Content analysis of qualitative data revealed following recurrent themes: Need
for integration highly valued; Delivery of curriculum can be further improved; Faculty is supportive; Small group
sessions encourage individual participation and active learning; Self directed learning prepares for life-long
learning; Logistics should be improved. Conclusions: Integrated curriculum provided holistic approach to
human problems and enhanced students’ motivation for patient centered and self-directed life-long learning6.
Take-home messages: Integrated curriculum can be implemented in a resource constrained environment;
however, it demands a strong institutional leadership role.
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213
Folk medicine in Turkey
K. Hüsnü Can BASER1, Ekrem SEZIK2
1
Formerly Anadolu University, Faculty of Pharmacy, Department of Pharmacognosy, 26470 Eskisehir, Turkey;
2
Gazi
University, Faculty of Pharmacy, Department of Pharmacognosy, Ankara, Turkey
Turkey is a country having land in two continents: Asia and Europe, rich in botanical and cultural diversity.
Flora of Turkey is rich and diverse with over 12.000 flowering plant taxa recorded enjoying an endemism
record of over 30 per cent. It is a land which hosted numerous civilizations and has witnessed various
successes in medicine, agriculture, history, etc. Cuneiform tablets dating back to Assyrians, Sumerians
and Hittites are witness to the use of many medicinal plants which are still used in the same way.
Hippocrates, Galenus, Theophrastus and Dioscorides contributed to the creation of a traditional form of
medicine in Anatolia 2000 years ago. Their followers like Avicenna, Rhazes, Biruni, Ibn Baitar, etc.
perfected medical and medicinal practices before the rise of the Renaissance. Their teachings influenced
medical practices in Turkey through the Seljukian and Ottoman era. Apart from the discovery of medicinal
plants of Anatolia by the natives, many nations settling in Anatolia brought along their culture and
practices as well as the knowledge of medicinal plants to enrich the already wealthy culture of the land.
The paper intends to to shed light to folk medicinal practices at work in today’s Turkey with examples
such as the Ocak (read Odjäck) system which is thought to be evolved as a result of islamic influences
on Central Asian shamanic rituals.
A-292
Influence of motor activity on health condition of students in Avicenna Tajik State
Medical University
Sh.Sagdieva 1
1Avicenna
Tajik State Medical University, Rudakiave, 139, Dushanbe, Tajikistan
Objective: To study the influence of impellent sport activities on students’ health condition. Methods:
The health level estimation of students has been studied. The health level estimation was studied using
Rufye index in 2 groups of students: 1) students visiting sport sections and living active physical life. 2)
students with no active physical activity. Results: The comparative analysis of the results has shown that
none of the groups had achieved excellent results. The good result in group of the boy students visiting
sports sections was 9.1% and in control group 0%. The satisfactory result in investigated group was
63.6% against 72.2% in control. The unsatisfactory result in group of sportsmen was 27.3% comparing to
72.7% in group of the students ignoring physical activities. Girls groups couldn’t achieve perfect and good
results at all. Satisfactory results had been registered in 36.4%, and the unsatisfactory result were 63.6%
in group of girls without regular physical activities. The group of the city girls who have regularly physical
activities, included only 4 persons aged 18–20 years, and among them there were no unsatisfactory
results. Conclusion: The preliminary results of our research confirm scientists opinion on a tendency to
deterioration of students’ health condition. The majority of students, who ignore physical activities, have
got proved unsatisfactory health condition.
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214
Innovative Strategies in Medical Education and Challenges faced in Establishing Gulf
Medical University in the Middle East – A Case Study
Vinod Abraham1
1Director
- External and International Affairs, Gulf Medical University, Ajman, U.A.E.
The Gulf Medical University (Formerly Gulf Medical College) and G.M.C. Hospital & Research Centre,
Ajman, established in 1998 has been involved in undergraduate education in Medicine, Physiotherapy,
Dentistry, Pharmacy and Premium Healthcare delivery since the last decade. The Gulf Medical University
is in its 14th year of providing quality Medical Education and is recognized as an institution of higher
education by the Ministry of Higher Education & Scientific Research, U.A.E. The University receives
students from 50 countries around the world. The GMU also is proud of its Innovative Strategies in
Medical Education; Organ System Based Curriculum developed and implemented by GMU in U.A.E..
Curriculum to feature simulation based clinical training. Unique Summer Training Programs and the
worldwide network of institutions to which the students can do their Summer Training and Internship. The
University is also a trendsetter in hosting the Regions first Dedicated Health Communications Divisions
publishing a Health Magazine in English & Arabic and the Regions First Conference Secretariat which has
done over 400 Major Conferences, Seminars, Workshops and other CME events. The Gulf Medical
University has innovated an ambitious plan of introducing Short Term Courses and Certification programs
targeted at the UAE’s working medical and allied health professionals. This presentation aims to highlight
a case study on the Innovative Strategies in Medical Education and Challenges faced in Establishing Gulf
Medical University in the Middle East.
A-294
Knowledge, Attitude and practice of medical ethics of faculty of a medical university, in
Karachi, Pakistan
Zulfiqar Ali Shaikh1, Masood Hameed Khan2, Dur Muhammad Shaikh3, Fahmeeda Khatoon4, Baqar Ali5,
Umer Bashir6, Hassan Shabir7
1MBBS,
MSPH, MBE; Associate Professor, Community Medicine, 2FCPS, FRCP, PhD; Professor, Medicine, 3MBBS, MPhil;
Assistant Professor, Pathology, 4MBBS, MPhil; Lecturer, Bio-chemistry,
5-7Medical
Students, Final Year, Institute: Dow
University of Health Sciences, Karachi, Pakistan. Correspondence: Dr Zulfiqar Ali Shaikh, Community Medicine Department,
Dow International Medical College / Dow University of Health Sciences, At Ojha Campus, SUPARCO Road, Karachi, Pakistan.
Email: drzulfiqarshaikh@gmail.com, Contact # +92-333-2356234
Background: The ethics in medical practice is as old as the profession itself. In many countries bioethics
is part of medical curriculum. Due to increased public health awareness, complaints against unethical
behavior of medical students and professionals are proliferating. Though bioethics has gained priority in
formal medical education, but is not much recognized everywhere. To design an ethical curriculum, the
first step is to assess the basic knowledge and attitude of medical teachers regarding bioethics, and
second to well equip them to impart same skills to the students to workout ethical dilemmas. This study
attempted to explore the knowledge about medical ethics in a Medical University of Karachi, Pakistan.
Objectives: To assess knowledge, attitude and practice of medical teachers regarding medical ethics,
and to sensitize the academicians for training of the teachers and making bioethics an integral part of the
215
curricula. Results: All of the study participants conducted research in their field of specialty; to get
promotion (71%), get funding (9%), discover new ideas/theories (4%), for personal interest (5%), and
multiple reasons (11%). Among them, 16% had received training of research / bioethics, and only 2% got
refresher training. Eighty two percent knew the existence of ethical review committee in their institution;
55% got prior approval from ERC, 32% declared conflict of interest, 17% taught/discussed medical ethics
with students; 21% took informed consent, 35% had idea of vulnerable population, 43% did not want to
give full information to the study participants, and 70% recognized difference between medical research
and care. According to 77%, the authors’ list included only those who had contributed, but 17% also
included supervisors/higher seniors and 6% colleagues. Conclusion: Health care providers frequently
encounter ethical issues. The medical teachers were not fully equipped with appropriate knowledge of
bioethics. It should be included in curriculum and teachers be trained for the same.
A-295
QOL assessment and proposed scale for evaluation of traditional medicines
Nobuo Yamaguchi1,3 , N.Amat 2, Y.Delitiat 2
1Medican
Research Institute of Xinjiang Medical University (830011); 2Uighur Medicine Department of Medical University Road
No. 393, Urumqi City , Xinjiang Uighur Autonomous Region , China. Visiting Professor, Xinjiang Medical University, Urumqi,
China; 3Ishikawa Natural Medicinal Products Research Center, Kanazawa, Ishikawa, 920-0167, Japan
According to the reports from Ministry of Health in each country, the average life spans were expanded
over the past century. This trend is expected to be extended further in the future and at the same time
continuous efforts should be made. That indicates that the quantitative objective, as one of the
objectives people have aimed at since ancient times to attain the perpetual youth & longevity, are almost
achieved in this way. However, as the next objective, it is necessary for us to be concerned about
improving the quality of life.
A-296
Search for bioactive compounds to inhibit plant pathogenic organisms
Narong Singbuaraudom1
1Department
of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand
Objective: One hundred Thai medicinal plants were evaluated for their in vitro anti-plant pathogenic
activity. Methods: Ethanol crude extracts were obtained and tested for anti mycelia growth by dilution
methods. The crude extracts that exhibited effective concentration lower than 10,000 ppm were selected
for isolation of the active compounds by column chromatography. Active fractions were screened by
diffusion method. The structures of the isolated compounds were characterized by NMR techniques.
Results: The results of this investigation are as follows; (1) the crude extract from Alium sativum was
highly effective in inhibiting plant pathogens, presumably due to allicin. Since allicin is unstable it
represents a low potential for development as commercialized product (2) berberine, the alkaloid isolated
from Coscinium fenestratum (Gaertre) Coleb exhibited specific ability to inhibit the grape scab fungus,
Sphaceloma ampelinum, and banana wilt, Fusarium oxysporum ssp. cubense. This extract is now in the
process of developed for commercialization in the name Natural-A .(3) chavicol, isolated from Piper betle
216
L., was found to exhibit a broad spectrum to inhibit plant pathogenic organisms. It is one of the
commercialized products which was name Natural-B. The other members of Piperaceae, namely Piper
niqrum and P.chaba, also contained bioactive compounds. Conclusion: The result of fractionation
revealed that these active compounds are different from those found in other members of this family
A-297
Secondary metabolites from a Türkish marine sponges
B.Konuklugil, 1 P.Proksch, 2 B.Gözcelioğlu 3
1University
of Ankara, Faculty of Pharmacy,06100- Tandoğan, Ankara, Turkey; 2Heinrich-Heine-Universität, Institute of
Pharmaceutical Biology, Universitätsstr. 1, 40225 Düsseldorf, Germany; 3Tübitak, Ankara, Turkey
The sponges represent rich source of secondary metabolites having a great diversity of structures and
bioactivities. They are even considered to be more prolific producers of new marine natural products.
Until now, more than 5000 different compounds have been isolated from about 500 species of sponges
and every year hundreds of new compounds are being discovered. Most bioactive compounds from
sponges can be classified as anti-inflammatory,anti-tumour, immuno- or neuro-suppressive, antiviral,
antimalarial, antibiotic and antifouling (1-4). Turkish coatline totals almost 8400km. The sponges found in
Türkish water, have not been intensively studied yet. Chemical investigation of methanolic extract of the
sponges led to isolation of furanosesterterpene, named fasciculatin, heptaprenylhyroguinone, avarol,
avarone, oroidin, 4,5-dibromopyrrol-2-carboxylic acid, and 25-hydroxy-24-methylcholesterol, agelasine
C, agelasine D and cylindradines A. The compounds were purified by “coloumn “ chromatograpy and their
structures was characterized using NMR spectroscopy. Some of these compounds were tested for
antioxidant, antimicrobial; anticholinesterase; and anti-trypanosomal activities. Futher studies are in
progress.
A-298
Study of Prevalence of Asthma in male workers of Leather Tanneries in Lahore, Pakistan
Ali, Sajid, Shehzad
Objective: This study aimed to estimate the prevalence and to identify some risk factors of adult asthma
in male workers in Lahore, Pakistan. Methods: A cross sectional study was conducted from August 2010
to March 2011 on leather tannery workers of Lahore, Pakistan. Data were collected from 641 workers
engaged in 95 different tanneries in industrial area selected as sample of convenience. Face to face
interviews were performed using a structured pre-tested questionnaire by trained data collectors.
Results: Prevalence of adult asthma was 10.8% (69/641) in this study population. The prevalence of
perceived work-related asthma was 5.3% (34/641). Multivariable logistic regression model showed that
after taking into account the age effect, the leather tannery worker were more likely to be asthmatic, if they
were illiterate (adjusted OR = 2.13, 95% CI: 1.17–3.88), of Pathan ethnicity (adjusted OR = 2.69; 95% CI:
1.35–5.36), ever-smoked (adjusted OR = 2.22, 95% CI: 1.16–4.26), reportedly never used gloves during
different tanning tasks (OR = 3.28; 95% CI : 1.72–6.26). Also, the final model showed a significant
interaction between perceived allergy and duration of work. Those who perceived to have allergy were
more likely to have asthma if their duration of work was 8 years (adjusted OR = 2.26; 95% CI: 1.19 – 4.29)
217
and this relationship was even stronger if duration was 13 years (adjusted OR = 3.67; 95% CI: 1.98–6.79).
Conclusion: Prevalence of asthma in leather tannery workers appears to be high and is associated with
educational status, ethnicity, smoking, glove use, perceived to have allergy and duration of work.
A-299
Some questions of the development of Uzbek public medicine and practical aspects of it
on the public health of the population
M.Khamraev
The academy of national medicine of Uzbekistan
The modern medicine has huge luggage of worked out procedures, doctrines, technologies. But the
advertised medicines can not cure us from all deceases at once. In this connection necessity for
formation the concept of maintenance of health has matured, in which both reforms of measures on
prophylaxis and avoidance of diseases, and management on incorporation in life of experience of the
Uzbek people would be reflected. The important moment in work should be a complex of improving
measures, which also includes daily work on informing the population about the able-bodied mode of life,
about methods of right self treatment, about the economic significance of health.We will discuss the the
purposes, problems and aspects of the National Medicine Program of Uzbikistan.
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YNSA as a new acupuncture treatment for pain killing
Hegyi Gabriella1
1
Head of CAM Department of PECS Medical University, Hungary, Director of Yamamoto Rehabilitation Institute, Budapest, HU
(affiliated to PECS Med. Uni.)
Introduction: Patients treated with YNSA often experience long-term relief from symptoms. Objective:
To evaluate the effectiveness of YNSA for the relief of pain of the locomotor system. Design, Setting, and
Patients: Prospective series of 2100 patients with severe movement-associated pain who were treated
with a single application of YNSA via basic points and Y points selected according to the palpation results
of a neck diagnosis. Depending on the duration of topometry, the needles were left in position for 3-9
minutes, or was used an unique permanent technique ( absorbeant monofilament with a special designed
needle to inset it) An objective measurement was used to record patients' movements by an external
computer-controlled ultrasonic emitter with an accuracy in the millimeter range. The participants
described their subjective sensation of pain before and after treatment via a visual analog scale (VAS) for
pain. All patients were interviewed a few weeks later to document the success of the treatment by
independent MDs.Main Outcome Measures: The speed of YNSA efficacy and its enduring effects, the
complaints for which it is particularly effective, and whether YNSA is suitable for emergency application.
Results: All together, 91,2% of the patients regarded a single, brief application of YNSA as effective.
The average VAS score before YNSA was 63/100; afterward the average was 19/100. A total of 51% of
the patients stated that they experienced complete relief of symptoms subjectively and by using the VAS.
A total of 43.3% of the patients (n=45) experienced some relief after YNSA. Seven patients did not
subjectively experience any effect. A total of 52.5% of the patients experienced both objectively
218
measurable (topometry) and subjectively stated relief or complete elimination of symptoms (on VAS),
lasting from 1 hour to 382 days. Overall movement after YNSA remained constant in topometric
measurements for 35.1%. Overall movement for 6,6% measured topometrically was worse after YNSA
than before. Conclusions: YNSA can be an effective treatment especially for pain of the locomotor
system originated from different reasons and can provide subjective improvement for most patients. Due
to its ease of application and its reliability, YNSA is particularly well suited for use in emergency treatment
since a good long-term effect can be achieved by leaving the needles in position for just 3-9 minutes or
more time in form of a permanent application for patients.
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Prevalence of gout in Pakistan
Sajid
This study was conducted to assess the prevalence of gouty arthritis in 100 patients (both male and
female) between the ages of 35 and 70 years. The patients that were randomly enrolled in this study The
mean age of the 66 male patients was 53.59 with a standard deviation of 11.34 and the mean age of the
34 female patients was 58.59 with a standard deviation of 8.19. Consequently, this indicated high
prevalence of gout in male than in female. It can be seen that between 35 and 40 years of age, the total
number of patients was 11; between 40 and 45, it was 12; between 45 and 50, it was 13; between 50 and
55, it was 13; between 55 and 60, it was 15; between 60 and 65, it was 19; and between 65 and 70 years
of age, it was 17. However, this clearly indicated that prevalence of gout was high in patients that are
above 50 years of age.
A-302
Traditional Uighur Medicine theory of "KuWat" (energy) classification and its function
T.Tursun 1，R.Kawuz 1
1Department
of Uyghur Medicine, Xinjiang Medical University, Urumqi, 830011, China; *Correspondence should be addressed
to turhun@263.net
Theory of "Kuwat",is one of the basic theory of Uighur Medicine, Is a description of human power
definition, types and functions. Dimensional medicine in force, refers to maintain and advance the human
mental and physical Major Factor. According to the " base " of the emergence and existence of position
and action,its divided into two energetically，
The former is ( Kuwat ) and the latter ( Kuwat ). KuWait (
former energy )，
The former is ( Kuwait ) the creation and existence of the position and role, divided into
vitality, spiritual force and natural force three categories. First, Kuwait is energy of life, vitality, refers to
promote heart beating force. It is produced in the heart, derived from the heart, for the normal function of
the heart service, and through the heart of the activities and complete, small blood circulation, for the
maintenance of normal human survival play an important role in. Although people's joy, anger, grief, joy
and emotion control ability is the effort to complete, but these mental phenomena in actual performance in
the heart. Second，
Kuwait is energy of spirit，
mental stress, refers to the generating and directing all
intelligence and force. It is produced in the brain, is impels all spiritual center.
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A-303
Experience of «RAWAN» application at sick children with bronchial asthma in
Turkmenistan
Dmytriy Sebrebryakov1
1Turkministan
State Medical University, Ashkabad, Turkministan
Bronchial asthma (BA) belongs to number of widespread allergic diseases at child age. During the last
years all over the word. including Turkmenistan, there is marked a tendency to increase in prevalence of
bronchial asthma at children and to its more serious course. Bronchial asthma is a disease which having
begun at children, often proceeds at mature age, becoming a cause of physical inability. In this
connection, the problem of prevention, diagnostics and treatment of this disease acquires a great value.
The purpose of this research was application of «RAWAN», made in Peoples Republic of China by
pharmaceutical firm "Ha-Bo", Urumchi SUAR, possessing property to strengthen «Wei-ci», inmmune
system, to eliminate weakness, constant hyperhidrosis, propensity to catarrhal diseases ,chill, tickling
and discomfort in a throat. There were surveyed 45 children at the age from 5 to 14 years. At 40％
of
patients there diagnosed BA of moderate severity, at 56％
-light course. At the majoriy of children with BA it
was combined with food medicinal allergy, pollinosis, atopic dermatitis, allergic rhintis. Alongside with a
basic treatment, there was appointed «RAWAN», three times a day of 3-5gr.dissolved in warm boiled
water. Course consisted of 25 days of treatment. In the research there were incluled sickly and long ailing
children with BA at which it was marked more than 4-6 episodes of ARVI in a year, accompanied by BA
aggravation. There was carried out monitoring of following indicators: serum interferon and α-interferon,
and also clinical dispalys of the disease. At the picture there is presented a level of interferon at children
receiving «RAWAN» there is noted decrease of serum interferon, initially raised, upon termination of
therapy(p<0.01) and increase of production of α-interferon(p<0.001) to the termination of a complete of
therapy. Clinical signs and sysptoms at children with bronchial asthma, receving«RAWAN». After the
termination of therapy with inclusion of «RAWAN». frequency of revealing of the specified symptoms
authentically decreased(p<0.01-0.001) in comparison with the revealed symptoms of initiation of
treatment, It is necessary to notice that at application of «RAWAN». At one of children receiving a
medicine, it is noted negative collateral and allergic displays. Thus, inclusion in complex therapy of
patients with BA of medicine«RAWAN»,has allowed to gain convincing clinical effect, The received
results on medicine application in treatment of patients with BA, are represented rather perspective.
Medicine dose not cause collateral and allergic reactions, changes in gastroenteric tract of observable
children at medicine application. Against medicine application production increase of production of
α-interferon initially lowered (p<0.001),and decreased in the raised level of serum interferon.
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Clinical effecacy observation Uighur Medicine Preparetion Denar on the patients with
viral hepatitis with Cholestatic syndrome
220
Dmytriy Sebrebryakov1
1Turkministan
State Medical University, Ashkabad, Turkministan
The treatment of cholestatic forms of viral hepatitis is the one of the complicated question in hepatology,
the purpose of this reasearch is application of the Uighur Medicine Preparetion Denar decoction treatment
the hepatitis with Cholestatic syndrome, Denar decoction can reliefe the fever of the liver, remove toxin,
remove sputum, functionging as a choleretic , laxative and uriginative agent. There was 39 patients with
chronic viral hapatitis with cholestatic sysndrome was under observation. The 15 of them were with
chronic viral hapatitis HBV+HDV, 18 with the chronic viral hapatitis HBV+HCV, and 6 with the chronic viral
hapatitis HBV.The diagnosis was verified by determination of infection marker of viral hepatitis.Along with
the basis treatment the “Denar ” was prescribed three times a day by 3-5g deluated with warm water. The
course of therapy consisted of 21 days. The control group conisited of 20 patients with the cholestatic
form of chronic viral hepatitis during the basic therapy. The effeciency of therary was evaluated by
laboratory data ,there was noted amelioration during the usage of “Denar”. Many of the patients faster
then in control. Lowering of the skin itch intensity, normalize sleeping. During the treatment,
Ictertitiousness of the coverlet sypntom of 30 of patients had decreased or then disappeared, and fast
reverse dynamics of biochemical tests, for the 28 patients the biochemical markers was improved, also
correlated with immune parameter wa normalized. 9 patients at the end of treatment had the marker of the
viral hepatitis decreased or show negative (superficial antigin of viral hepaptitis HBV, antibodies IgM to
the heart shaped antigen of viral hepaptitis HBV in the immune-enzyme analysis) and there was no
datected nucleic acids of viruses in polymerase chain reaction. We also found that can not find any side
effection and allergic affection. Thus, the inclusion to the complex therapy preparation “Denar” allowed to
get convincing clinical effect. The obtained results in application of this medicine in treatment of viral
hepaptitis with cholestatic syndrome syndrome present itself very prespective.
A305
Epidemiological analysis of vitiligo according to traditional Uyghur medical theory and
western medicine
K.Tuohuti 1, R.Shawuer 1*
1Hotan
prefecture traditional Uyghur medical hospital, Xinjiang autonomous region PRC，hetian City，848000, correspondent to
kuraxht@yahoo.com.cn
Objective：To analyze Epidemiology of vitiligo according to traditional Uighur medical theory (one of the
four big traditional medical systems next to TCM, Tibet and Mongol in china. an organic combination of
traditional medical system in Islamic east ,china with Far East) and western medicine. Method: with 80
clinical cases we used Uyghur medical theory about albino pathology and epidemiological parameter (age,
sex, family history, work place- Job and regional difference ) of western medicine. Result: 1. according to
pathology analysis albino with help of humour theory of traditional Uighur medical system it seems people
with phlegmatic structure especially with tasteless phlegm (temsiz belgam) and salty phlegm (tuzluq
belgam). 2. People with eat habit cold nature foods example (yoghurt) has more clinical cases 3. During the
school age girls have high risk. Vitiligo cases seem more frequent in girls than boys. 4. Relationship with job.
Intellectuals have more cases than works and farmers. Teachers and pupils are high risk groups.4: regional
difference: following is regional frequency comparison among four places. Hotan < Aksu < kashgar < ili.
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Conclusion: systematic epidemiological finding is important to prevent and cure vitiligo. Our research would
be some contribution to cure vitiligo in parts of undeveloped regions in china and other world with herbal
medicine.
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