Structural basis for recognition of an unnatural base pair by T7 RNA polymerase
Yasushi Hikida1,2, Shun-ichi Sekine1,2, Michiko Kimoto1, Ichiro Hirao1, and Shigeyuki
Yokoyama1,2
(1RIKEN, 2The University of Tokyo)
Unnatural base pairs are being developed worldwide to utilize them as the “third” base pair in
replication, transcription, translation, and so on, together with the two Watson-Crick base pairs1.
T7 RNA polymerase is frequently used to transcribe unnatural bases in DNA to RNA. In the
present study, we determined the crystal structure of a complex of T7 RNA polymerase with a
DNA/RNA scaffold containing unnatural bases.……
References
1. M. Kimoto and I. Hirao, “Development of Unnatural Base Pairs”, J. Unnatural Base Pairs, 1,
1–10 (2014).
2. …