QBC Diagnostics, Inc.
Robert A Levine, M.D. Email: qbc@aol.com
General review of the use of QBC for detection of
Hematoparasites with hypertext linked references
Quantitative Buffy Coat (QBC) analysis of blood for the detection of
hematoparasitic diseases was first invented in 1980 by Levine and Wardlaw.
The first clinical trial utilizing this technology for the diagnosis of a
hematoparasitic disease, malaria, was performed in 1988.
Since that time, without any organized effort, hundreds of articles and
presentations have documented the utility of QBC as a simple, accurate and
highly sensitive method of detecting the hematoparasites that cause, malaria, tick
borne relapsing fever, sleeping sickness, filariasis, babesiosis and visceral
leishmaniasis and most recently congenital, acute Chagas Disease as well as
recrudescences of Chagas disease in patients with AIDS.
The QBC technology is being improved so that vital hematological parameters,
the hematocrit and platelet count, may be obtained at the same time as the
diagnostic test is performed at no additional expense with only seconds of added
time. These parameters are very important in clinical decisions and treatment.
Anemia and or thrombocytopenia are important indicators of severe illness. A
new battery powered, LED illuminated, and much less expensive ParaViewer is
planned.
There is no other technology that can diagnose all of the above hematoparasitic
diseases with one test, at the same time, and have all the results including the
hematology parameters available at the point of care. Patients do not ask if they
have malaria, they ask to be diagnosed properly, promptly and accurately so that
they may be properly treated. Only the QBC can accomplish this.
Diagnosis of hematoparasites using quantitative buffy coat
analysis (QBC)
Levine RA, Wardlaw SC, Patton CL.
Related Articles, Links
Detection of haematoparasites using quantitative buffy coat analysis tubes.
Parasitol Today. 1989 Apr;5(4):132-4. No abstract available.
PMID: 15463196 [PubMed - in process]
Spielman A, Perrone JB, Teklehaimanot A, Balcha F, Wardlaw SC,
Levine RA.
Related Articles, Links
Malaria diagnosis by direct observation of centrifuged samples of blood.
Am J Trop Med Hyg. 1988 Oct;39(4):337-42.
PMID: 3189696 [PubMed - indexed for MEDLINE]
QBC Diagnostics, Inc.
Robert A Levine, M.D. Email: qbc@aol.com
Tick-borne relapsing fever (Borrelia)
1
Lecompte Y, Trape JF.
Related Articles, Links
[West African tick-borne relapsing fever]
Ann Biol Clin (Paris). 2003 Sep-Oct;61(5):541-8. Review. French.
PMID: 14671751 [PubMed - indexed for MEDLINE
2
Chatel G, Gulletta M, Matteelli A, Marangoni A, Signorini L, Oladeji O,
Caligaris S.
Related Articles, Links
Short report: Diagnosis of tick-borne relapsing fever by the quantitative buffy coat
fluorescence method.
Am J Trop Med Hyg. 1999 May;60(5):738-9.
PMID: 10344644 [PubMed - indexed for MEDLINE
3
van Dam AP, van Gool T, Wetsteyn JC, Dankert J.
Related Articles, Links
Tick-borne relapsing fever imported from West Africa: diagnosis by quantitative
buffy coat analysis and in vitro culture of Borrelia crocidurae.
J Clin Microbiol. 1999 Jun;37(6):2027-30.
PMID: 10325370 [PubMed - indexed for MEDLINE]
Cobey FC, Goldbarg SH, Levine RA, Patton CL.
Related Articles, Links
Short report: Detection of borrelia (relapsing fever) in rural Ethiopia by means of the
quantitative buffy coat technique.
Am J Trop Med Hyg. 2001 Aug;65(2):164-5.
PMID: 11508395 [PubMed - indexed for MEDLINE]
Hovette P, Aubron C, Perrier-Gros-Claude JD, Schieman R, N'Dir MC,
Camara P.
Related Articles, Links
[Value of Quantitative Buffy Coat (QBC) in borreliasis-malaria co-infection]
Med Trop (Mars). 2001;61(2):196-7. French. No abstract available.
PMID: 11582881 [PubMed - indexed for MEDLINE]
QBC Diagnostics, Inc.
Robert A Levine, M.D. Email: qbc@aol.com
Malaria
Jain M, Kaur M.
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Comparative study of microscopic detection methods and haematological changes in
malaria.
Indian J Pathol Microbiol. 2005 Oct;48(4):464-7.
PMID: 16366095 [PubMed - indexed for MEDLINE]
Malik S, Khan S, Das A, Samantaray JC.
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Plasmodium lactate dehydrogenase assay to detect malarial parasites.
Natl Med J India. 2004 Sep-Oct;17(5):237-9.
PMID: 15638301 [PubMed - indexed for MEDLINE]
Nandwani S, Mathur M, Rawat S.
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Evaluation of the direct acridine orange staining method and Q.B.C. test for
diagnosis of malaria in Delhi, India.
J Commun Dis. 2003 Dec;35(4):279-82.
PMID: 15909757 [PubMed - indexed for MEDLINE]
Barman D, Mirdha BR, Samantray JC, Kironde F, Kabra SK, Guleria R.
Related Articles, Links
Evaluation of quantitative buffy coat (QBC) assay and polymerase chain reaction
(PCR) for diagnosis of malaria.
J Commun Dis. 2003 Sep;35(3):170-81.
PMID: 15796409 [PubMed - indexed for MEDLINE]
Yavo W, Ackra KN, Menan EI, Barro-Kiki PC, Kassi RR, Adjetey
TA, Bamba A, Kone M.
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[Comparative study of four malaria diagnostic techniques used in Ivory Coast]
Bull Soc Pathol Exot. 2002 Nov;95(4):238-40. French.
PMID: 12596367 [PubMed - indexed for MEDLINE]
Krishna BV, Deshpande AR.
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Comparison between conventional and QBC methods for diagnosis of malaria.
Indian J Pathol Microbiol. 2003 Jul;46(3):517-20.
PMID: 15025329 [PubMed - indexed for MEDLINE
Pinto M JW, Rodrigues SR, Desouza R, Verenkar MP
QBC Diagnostics, Inc.
Robert A Levine, M.D. Email: qbc@aol.com
Department of Microbiology, Goa medical college, Bambolim, Goa - 403
202, India
Click here: Usefulness of quantitative buffy coat blood parasite detection system in
diagnosis of malaria Pinto M JW, Rodrigue
.
Indian Journal of Medical Microbiology,
Year : 2001 | Volume : 19 | Issue : 4 | Page : 219-221
Singh H, Tyagi PK, Sharma SK.
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Malaria diagnosis: quantitative buffy coat versus conventional microscopy.
J Assoc Physicians India. 2001 Sep;49:945-6. No abstract available.
PMID: 11837776 [PubMed - indexed for MEDLINE]
Hovette P, Aubron C, Perrier-Gros-Claude JD, Schieman R, N'Dir MC,
Camara P.
Related Articles, Links
[Value of Quantitative Buffy Coat (QBC) in borreliasis-malaria co-infection]
Med Trop (Mars). 2001;61(2):196-7. French. No abstract available.
PMID: 11582881 [PubMed - indexed for MEDLINE]
Thakor HG.
Related Articles, Links
Laboratory diagnosis of malaria.
J Indian Med Assoc. 2000 Oct;98(10):623-7.
PMID: 11258491 [PubMed - indexed for MEDLINE]
Biswas R, Sengupta G, Mundle M.
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A controlled study on haemograms of malaria patients in Calcutta.
Indian J Malariol. 1999 Mar-Jun;36(1-2):42-8.
PMID: 11304918 [PubMed - indexed for MEDLINE]
Mirdha BR, Samantray JC, Burman D, Mishra B, Ghimire P.
Related Articles, Links
Quantitative buffy coat: a special adjunct for diagnosis of malaria.
J Commun Dis. 1999 Mar;31(1):19-22.
PMID: 10810581 [PubMed - indexed for MEDLINE]
el Serougi AO, Amin AM.
Related Articles, Links
The quantitative buffy coat capillary tubes versus thin and thick blood films in the
diagnosis of malaria in Saudi Arabia.
J Egypt Soc Parasitol. 1998 Apr;28(1):17-22.
PMID: 9617039 [PubMed - indexed for MEDLINE
QBC Diagnostics, Inc.
Robert A Levine, M.D. Email: qbc@aol.com
Damodar SU.
Related Articles, Links
Evaluation of acridine-orange staining of centrifuged parasites in malarial infection.
Indian J Med Sci. 1996 Jul;50(7):228-30.
PMID: 8979539 [PubMed - indexed for MEDLINE]
Wang X, Zhu S, Liu Q, Hu A, Zan Z, Yu Q, Yin Q.
Related Articles, Links
Field evaluation of the QBC technique for rapid diagnosis of vivax malaria.
Bull World Health Organ. 1996;74(6):599-603.
PMID: 9060220 [PubMed - indexed for MEDLINE
Bawden M, Malone J, Slaten D.
Related Articles, Links
QBC malaria diagnosis: easily learned and effectively applied in a temporary
military field laboratory.
Trans R Soc Trop Med Hyg. 1994 May-Jun;88(3):302. No abstract available.
PMID: 7974669 [PubMed - indexed for MEDLINE]
Long GW, Jones TR, Rickman LS, Fries L, Egan J, Wellde B, Hoffman SL.
Related Articles, Links
Acridine orange diagnosis of Plasmodium falciparum: evaluation after experimental
infection.
Am J Trop Med Hyg. 1994 Nov;51(5):613-6.
PMID: 7985754 [PubMed - indexed for MEDLINE]
Visceral Leishmaniasis
Liarte DB, Mendonca IL, Luz FC, Abreu EA, Mello GW, Farias TJ, Ferreira
AF, Millington MA, Costa CH.
Related Articles, Links
QBC for the diagnosis of human and canine american visceral leishmaniasis:
preliminary data.
Rev Soc Bras Med Trop. 2001 Nov-Dec;34(6):577-81.
PMID: 11813066 [PubMed - indexed for MEDLINE
Trypanosomes
Louis FJ, Buscher P, Lejon V.
[Diagnosis of human African trypanosomiasis in 2001]
Med Trop (Mars). 2001;61(4-5):340-6. Review. French.
PMID: 11803824 [PubMed - indexed for MEDLINE
Related Articles, Links
QBC Diagnostics, Inc.
Robert A Levine, M.D. Email: qbc@aol.com
Ancelle T, Paugam A, Bourlioux F, Merad A, Vigier JP.
Related Articles, Links
[Detection of trypanosomes in blood by the Quantitative Buffy Coat (QBC)
technique: experimental evaluation]
Med Trop (Mars). 1997;57(3):245-8. French.
PMID: 9513149 [PubMed - indexed for MEDLINE]
Ancelle T, Paugam A, Bourlioux F, Merad A, Vigier JP.
Related Articles, Links
[Detection of trypanosomes in blood by the Quantitative Buffy Coat (QBC)
technique: experimental evaluation]
Med Trop (Mars). 1997;57(3):245-8. French.
PMID: 9513149 [PubMed - indexed for MEDLINE]
Fischer P, Kilian AH, Bamuhiiga J, Kipp W, Buttner DW.
Related Articles, Links
Prevalence of Mansonella perstans in western Uganda and its detection using the
QBC-fluorescence method.
Appl Parasitol. 1996 Jan;37(1):32-7.
PMID: 8574245 [PubMed - indexed for MEDLINE]
Truc P, Bailey JW, Doua F, Laveissiere C, Godfrey DG.
Related Articles, Links
A comparison of parasitological methods for the diagnosis of gambian
trypanosomiasis in an area of low endemicity in Cote d'Ivoire.
Trans R Soc Trop Med Hyg. 1994 Jul-Aug;88(4):419-21.
PMID: 7570825 [PubMed - indexed for MEDLINE]
Bailey JW, Smith DH.
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The quantitative buffy coat for the diagnosis of trypanosomes.
Trop Doct. 1994 Apr;24(2):54-6.
PMID: 8009614 [PubMed - indexed for MEDLINE]
Bailey JW, Smith DH.
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The use of the acridine orange QBC technique in the diagnosis of African
trypanosomiasis.
Trans R Soc Trop Med Hyg. 1992 Nov-Dec;86(6):630. No abstract available.
PMID: 1287921 [PubMed - indexed for MEDLINE
QBC Diagnostics, Inc.
Robert A Levine, M.D. Email: qbc@aol.com
Leptospira
Kramer KJ, Pang LW, Minette HP, Perrone JB.
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Evaluation of the quantitative buffy coat analysis (QBC) system for the detection of
Leptospira in human blood.
Southeast Asian J Trop Med Public Health. 1994 Dec;25(4):788-9. No abstract available.
PMID: 7667735 [PubMed - indexed for MEDLINE]
Filariasis
Mylonakis ME, Papadopoulos E, Koutinas AF, Paitaki C, Leontides L.
Related Articles, Links
Comparative methodology for the detection and differentiation of circulating
microfilariae of Dirofilaria immitis in the dog.
J Helminthol. 2004 Jun;78(2):137-40.
PMID: 15153285 [PubMed - indexed for MEDLINE]
Freedman DO, Berry RS.
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Rapid diagnosis of Bancroftian filariasis by acridine orange staining of centrifuged
parasites.
Am J Trop Med Hyg. 1992 Dec;47(6):787-93.
PMID: 1281967 [PubMed - indexed for MEDLINE]
Cross JH.
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Recent advances in epidemiological field techniques in filariasis.
Southeast Asian J Trop Med Public Health. 1993;24 Suppl 2:40-4. Review.
PMID: 7973945 [PubMed - indexed for MEDLINE
Bawden M, Slaten D, Malone J.
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QBC: rapid filaria diagnoses from blood--Mansonella ozzardi and Wuchereria
bancrofti.
Trans R Soc Trop Med Hyg. 1994 Jan-Feb;88(1):66. No abstract available.
PMID: 8154007 [PubMed - indexed for MEDLINE
QBC Diagnostics, Inc.
Robert A Levine, M.D. Email: qbc@aol.com
Babesiosis
Bose R, Jorgensen WK, Dalgliesh RJ, Friedhoff KT, de Vos AJ.
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Current state and future trends in the diagnosis of babesiosis.
Vet Parasitol. 1995 Mar;57(1-3):61-74. Review.
PMID: 7597794 [PubMed - indexed for MEDLINE]
Morzaria S, Katende J, Kairo A, Nene V, Musoke A.
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New methods for the diagnosis of Babesia bigemina infection.
Mem Inst Oswaldo Cruz. 1992;87 Suppl 3:201-5. Review.
PMID: 1343691 [PubMed - indexed for MEDLINE
Mattia AR, Waldron MA, Sierra LS.
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Use of the Quantitative Buffy Coat system for detection of parasitemia in patients
with babesiosis.
J Clin Microbiol. 1993 Oct;31(10):2816-8.
PMID: 8253995 [PubMed - indexed for MEDLINE]