Supplementary Figure Captions
Supplementary Figure S1
Morphology of the nycteribiid bat flies examined in this study.
Bars show 1 mm.
Supplementary Figure S2
In vivo localization of the endosymbiont in Nycteribia allotopa
visualized by 16S rRNA-targeted fluorescent in situ hybridization. (A) An adult male; (B) an
adult female. Red signals indicated by arrows are the bacteriocytes harboring the
endosymbiont.
Supplementary Figure S3
Phylogenetic relationship of the endosymbionts of the
nycteribiid bat flies on the basis of 16S rRNA gene sequences. A neighbor-joining tree
inferred from a total of 1446 aligned nucleotide sites is shown, whereas maximum parsimony
and maximum likelihood analyses gave substantially the same results. Host insect names and
sample codes (see Supplementary Table S1) are labelled on the tree with sequence accession
numbers in brackets. Bootstrap values no less than 50% are indicated at the nodes in the order
of neighbor-joining/maximum parsimony/maximum likelihood, while asterisks indicate
support values less than 50%.
Supplementary Figure S4
Phylogenetic relationship of the endosymbionts of the
nycteribiid bat flies on the basis of gyrB gene sequences. A neighbor-joining tree inferred
from a total of 638 aligned nucleotide sites is shown, whereas maximum parsimony and
maximum likelihood analyses gave substantially the same results. Host insect names and
1
sample codes, and bootstrap values are shown as in Supplementary Figure S3.
Supplementary Figure S5
Phylogenetic relationship of the endosymbionts of the
nycteribiid bat flies on the basis of groEL gene sequences. A neighbor-joining tree inferred
from a total of 614 aligned nucleotide sites is shown, whereas maximum parsimony and
maximum likelihood analyses gave substantially the same results. Host insect names and
sample codes, and bootstrap values are shown as in Supplementary Figure S3.
Supplementary Figure S6
Phylogenetic relationship of the endosymbionts of the
nycteribiid bat flies on the basis of concatenated nucleotide sequences of 16S rRNA, gyrB and
groEL genes. A neighbor-joining tree inferred from a total of 2698 aligned nucleotide sites is
shown, whereas maximum parsimony and maximum likelihood analyses gave substantially
the same results. Host insect names and sample codes, and bootstrap values are shown as in
Supplementary Figure S3.
Supplementary Figure S7
Phylogenetic relationship of the nycteribiid bat flies on the basis
of mitochondrial cytochrome oxidase I gene sequences. A neighbor-joining tree inferred from
a total of 658 aligned nucleotide sites is shown, whereas maximum parsimony and maximum
likelihood analyses gave substantially the same results. Insect names and sample codes, and
bootstrap values are shown as in Supplementary Figure S3.
Supplementary Figure S8
Phylogenetic relationship of the nycteribiid bat flies on the basis
of mitochondrial 16S rRNA gene sequences. A neighbor-joining tree inferred from a total of
2
1544 aligned nucleotide sites is shown, whereas maximum parsimony and maximum
likelihood analyses gave substantially the same results. Insect names and sample codes, and
bootstrap values are shown as in Supplementary Figure S3.
Supplementary Figure S9
Phylogenetic relationship of the nycteribiid bat flies on the basis
of concatenated nucleotide sequences of mitochondrial cytochrome oxidase I and 16S rRNA
genes. A neighbor-joining tree inferred from a total of 2202 aligned nucleotide sites is shown,
whereas maximum parsimony and maximum likelihood analyses gave substantially the same
results. Insect names and sample codes, and bootstrap values are shown as in Supplementary
Figure S3.
Supplementary Figure S10 Pulsed-field gel electrophoresis of the endosymbiont genomic
DNA prepared from dissected bacteriomes of Penicillidia jenynsii. S, the DNA sample; M,
molecular size markers.
3