IMPERIAL COLLEGE LONDON JOB DESCRIPTION Job Title: Research Technician (SM207/09) Department: Surgery and Cancer Division: Cancer Job Family / Level: Technical Services, Level 3a Salary Range: £26,720 - £27,430 per annum Responsible to: Professor R. Brown and Dr J. Flanagan Contract: Full time for a fixed-term of two years Location: Hammersmith Hospital campus, East Acton Summary of Post: The post will be held within the Epigenetics section of the Division of Cancer and the holder will be jointly supervised by Dr Flanagan and Professor Brown. The post holder will work on DNA methylation profiling from samples of ovarian cancer patients as part of a Cancer Research UK funded study. The primary objective of the study is to examine association between clinical outcome and methylation of candidate loci predicted to show intra-individual variation in methylation or which are of particular relevance for ovarian cancer. This will be examined by bisulphite pyrosequencing of peripheral blood DNAs from ovarian cancer patients. There is a secondary exploratory objective using Differential Methylation Hybridisation of a customised microarray to identify novel variably methylated loci in ovarian cancer. DNA from ovarian cancer patients will be compared to age-matched unaffected controls using a 60K customised array that will focus on loci implicated in clinical outcome in ovarian cancer patients. Major Activities and Responsibilities: You will be primarily responsible for carrying out a range of DNA methylation analysis including bisulphite pyrosequencing and Differential Methylation Hybridisation under the supervision of Professor Brown and Dr Flanagan. You will be responsible for: taking an active role in project planning, design of methylation assays and technical problem solving coordination of collection and processing of patient and DNA samples analysis of samples by bisulphite pyrosequencing and related methodologies routine maintenance of the Pyrosequencer and quality control reactions preparing samples for differential methylation hybridization coordination of samples and analysis with colleagues and external partners the daily planning, execution and documentation of experimental work; implementing new technologies into the laboratory to increase throughput and scope of research active participation in laboratory meetings preparation of scientific data to be presented at internal and external meetings in relation to the project contribution to the intellectual life of the laboratory and the Division, as appropriate assistance with figures for manuscripts for publication organisation and participation in laboratory rotas for technical support duties in the Epigenetics Section obtaining the necessary safety training required for general laboratory safety/good practice, working with radioactive materials and handling genetically modified organisms if required familiarising yourself with the electronic ICIS ordering system; to keep up to date with technical advances and progress in relevant research areas assisting with COSHH risk assessments; and you may also be expected to offer support to other colleagues by providing advice and/or supervision (eg of undergraduate or M.Sc. projects as required). Job descriptions cannot be exhaustive and so the post holder may be required to undertake other duties, which are broadly in line with the above key responsibilities. The post holder is expected to observe and comply with all College policies and regulations, for example Health and Safety, Data Protection etc. Imperial College is committed to equality of opportunity and to eliminating discrimination. All employees are expected to adhere to the principles set out in our Equal Opportunities in Employment Policy, Promoting Race Equality Policy and Disability Policy and all other relevant guidance/practice frameworks. PERSON SPECIFICATION Qualifications: Essential Degree in Biological Science (preferably Biochemistry/Molecular Biology/ Biophysics/ Cell Biology) or equivalent research. Experience and Knowledge Essential Experience in methods of analysis of DNA methylation or DNA sequence analysis Good working knowledge of current concepts in eukaryotic gene expression mechanisms at an advanced level. Proven laboratory experience. Desirable Experience in pyrosequencing methods of analysis of DNA methylation or DNA sequence analysis. Experience of clinical studies of biomarkers. Skills and Abilities Essential Proactive, capable of initiating ideas / problem solving. Well developed interpersonal skills. Capable of prioritising the work load to meet deadlines. Well organised and able to work under pressure, while maintaining a positive approach to work and relationship with others. Willing to provide training and assistance to others. Proficient in the use of Microsoft Word, Excel and Powerpoint. APPLICATION GUIDANCE Please read the person specification carefully and describe, as part of your application, how much you feel you meet each of the criteria. Thank you for your interest in this post. We look forward to receiving your application.