RESUME
name:
Naiming Zhou
current address: Apt. 20-1-501
Liuxiang Road, Gangwan Jiayuan
Zhijingang Campus
Zhejiang University
Hangzhou, 310030, China
E-mail address: znm2000@yahoo.com
Tel:
86-571-88206748 (office)
86-13588743854 (cell)
EDUCATION
1977-1981: Suzhou University
1982-1985: Mast Degree. Zhejiang University
1989-1992: Doctoral Degree. Zhejiang University
PROFESSIONAL EXPERIENCE
Professor (Sep. 2006 - )
College of Life Sciences
Zhejiang University
Project: GPCRs signaling and GPCRs-targeted drug discovery
Senior Research Scientist (July 2002 – September 2006)
Incyte Corporation, Inc., Wilmington, DE
Project: Drug discovery and development of GPCR high-throughput screen assays
and function assays
Research Associate (Sept. 2000 – July 2002)
Medicine Department, Thomas Jefferson University, Philadelphia, PA
Project: Role of G-protein coupled receptors in HIV-1 infection.
Postdoctor (Jan. 1997- Sept. 2000)
Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, PA
Project: Structural and functional characterization of chemokine receptors
Postdoctor (Sep. 1993 – Jan. 1997)
Rega Institute, University of Leuven, Leuven, Belgium
Projects: Role of cytokine in autoimmune diseases
Guest Research Scientist (1991 – 1993)
Shanghai Institute of Biochemistry, Shanghai, China
Project: Molecular biology of Baculovirus and expression of proteins in insect cells
and larva
PUBLICATIONS
1. Guo Li, Xiaoyan Deng, Qi Zhou, Jiansong Luo, and Jeffrey Benovic, Naiming
Zhou. Distinct Kinetic and Spatial Patterns of Gi-Initiated PKC- and EGFRDependent Activation of Extracellular Signal-Regulated Kinases 1 and 2 by Human
Nicotinic Acid Receptor GPR109A. (2011) J. Biol. Chem. 286(36): 31199-31212.
2. Shin N, Solomon K, Zhou N, Wang KH, Garlapati V, Thomas B, Li Y, Covington
M, Baribaud F, Erickson-Viitanen S, Czerniak P, Contel N, Liu P, Burn T, Hollis
G, Yeleswaram S, Vaddi K, Xue CB, Metcalf B, Friedman S, Scherle P, Newton R.
Identification and characterization of INCB9471, an allosteric noncompetitive
small-molecule antagonist of C-C chemokine receptor 5 with potent inhibitory
activity against monocyte migration and HIV-1 infection. J (2011) Pharmacol Exp
Ther. 338(1):228-39.
3. Yaping Zhang, Wen Yang, Linjie Chen, Guo Li, Haishan Huang, Naiming Zhou.
Development of a Novel Assay Based on DnaE intein for Quantitative Analysis of
GPCR Internalization. (2011) Anal Biochem. 417(1):65-72.
4. Haishan Huang, Xiaoyan Deng, Xiaobai He, Wen Yang, Guo Li, Ying Shi,
Liangen Shi, Jimin Gao, and Naiming Zhou. Identification of Distinct C-terminal
Domains of the Bombyx Adipokinetic Hormone Receptor that are Essential for
Receptor Export, Phosphorylation and Internalization. (2011) Cell Signal.
23(9):1455-1465.
5. Haishan Huang, Guo Li, Liangen Shi, and Naiming Zhou. Activation of
Extracellular Signal-Regulated Kinase via Multiple Mechanisms by G-protein
couples Adipokinetic Hormone Receptor of Bombyx mori in Chinese Hamster
Ovary Cells. (2010) Biochemistry-US. 49(51):10862-10872.
6. Linjie Chen, Yaping Zhang, Guo Li, Naming Zhou. Functional Characterization of
a Naturally Occurring Trans-Splicing Intein from Synechococcus elongates in
Mammalian Cell System. Analytical Biochemistry 407:180-187.
7. Xiaopan Chen, Yi Fan, Wen Yang, Jufang Yan, Zheng Wang, Kun Hong, Xin
Chen, Jeffrey L. Benovic, Jiangxin Lu, Naiming Zhou. Structural Determinants in
the Second Intracellular Loop for Coupling of the Human Cannabinoid CB1
Receptor to Gαand Gαi Proteins. (2010) British Journal of Pharmacology
161:1817-1834.
8. Yi Fan, Peng Sun, Yu Wang, Xiaobai He, Xiaoyan Deng, Xiaopan Chen,
Guozheng Zhang, Xin Chen and Naiming Zhou. The G protein-coupled receptors
in the silkworm, Bombyx mori. (2010) Insect Biochemistry and Molecular Biology.
40(8):581-591.
9. Liu, Hong; Zhou, Yu; Jiang, Chunyi; Zhang, Yaping; Liu, Wenfeng; Zhong,
Tingting; Liang, Zhongjie; Sun, Yi; zhao, linxiang; Jiang, Hualiang; Zhou,
Naiming; Liu, Dongxiang; Luo, Cheng. Structural Optimization and Biological
Evaluation of Substituted Bisphenol A Derivatives as β-Amyloid Peptide
Aggregation Inhibitors. (2010) Journal of Medicinal Chemistry. 53(15): 5449-5466
(Co-corresponding author).
10. Guo Li, Ying Shi, Yaping Zhang, Jiansong Luo, Yi Sun, Jeffrey L.Benovic, and
Naiming Zhou.. Internalisation of the Human Nicotinic Acid Receptor GPR109A
is Regulated by Gi, GRK2 and β-Arrestin2. (2010) J. Biol. Chem. 285:2260522618.
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11. Chenggang Zhu, Haishan Huang, Rongsheng Hua, Guo Li, Yanyan Gu, Jiansong
Luo, Cunxin Zhang, Liangen Shi, Jeffrey L.Benovic, and Naiming Zhou.
Molecular and Functional Characterization of Adipokinetic Hormone Receptor and
its Peptide Ligands in Bombyx mori. (2009) FEBS Lett. 583:1463-1468.
12. Zhou N, Fang J, Mukhtar M, Acheampong E, Pomerantz RJ. Inhibition of HIV-1
fusion with small interfering RNAs targeting the chemokine coreceptor CXCR4.
(2004) Gene Ther. 11(23):1703-12.
13. Fang J, Kubota S, Yang B, Zhou N, Zhang H, Godbout R, Pomerantz RJ. A DEAD
box protein facilitates HIV-1 replication as a cellular co-factor of Rev. (2004).
Virology 330(2):471-80.
14. Zhou N, X. Zhang, X. Fan, E. Argyris, J. Fang, E. Acheampong, G. DuBoisand ,
-Terminal Domain of APJ, a CNS-Based Co-Receptor
for HIV-1, is Essential for its Receptor Function and Co-Receptor Activity. 2003,
Virology 317(1):84-94.
15. Fan X., N. Zhou, X. Zhang, M. Mukhtar, Z. Lu, J. Fang, G. DuBois, R. Pomerantz.
Structural and functional study of the apelin-13 peptide, an endogenous ligand of
the HIV-1 coreceptor, APJ. 2003, Biochemistry. 42(34):10163-8.
16. Zhou N, J. Fang, E. Acheampong, and R. Pomerantz. Binding of ALX40-4C to
APJ, the CNS-Based Receptor, Inhibits Utilization as Co-receptor by HIV-1. 2003,
Virology, 312(1):196-203.
17. Zhou N, X. Fan, M. Mukhtar, J. Fang, C.Patel, G. DuBois, and R. Pomerantz, Cellcell fusion and internalization of the CNS-Based, HIV-1 Co-Receptor, APJ. 2003,
Virology, 307(1):22-36.
18. Zhou N., Z. Luo, J. Luo, X. Fan, M. Cayabyab, M. Hiraoka, D. Liu, X. Han,
H.Kaji, J. Sodroski, and Z. Huang, Exploring the stereochemistry of CXCR4peptide recognition and inhibiting HIV-1 entry with D-peptides derived from
chemokine. 2002, J Biol Chem. 277 (20): 17476-85.
19. Zhou N., Luo Z, Luo J, Liu D, Hall JW, Pomerantz RJ, Huang Z, Structural and
functional characterization of human CXCR4 as a chemokine receptor and HIV-1
co-receptor by mutagenesis and molecular modeling studies. 2001, J Biol Chem.
276(46): 42826-33.
20. Luo Z., X. Fan, N. Zhou, M. Hiraoka, J. Luo, H. Kaji, and Z. Huang, Structurefunction study and anti-HIV activity of synthetic peptide analogues derived from
viral chemokine vMIP-II. 2000, Biochemistry. 39(44):13545-50.
21. Zhou, N., Z. Luo, J. Luo, J. Hall and Z. Huang, Synthetic peptides derived from
the N-terminus of the viral chemokine vMIP-II display distinct interactions with
CXCR4 and CCR5. 1999, Biochemistry, 39(13):3782-3787 (2000)
22. Luo J., Z. Luo, N. Zhou, J.W. Hall and Z. Huang, The attachment of C-terminus of
SDF-1 enhances the biological activity of its N-terminal peptide, 1999, Biochem.
Biophys. Res. Commun., 264(1):42-47.
23. Luo Z., N. Zhou, J. Luo, J. W. Hall and Z. Huang, The role of positively charged
residues in CXCR4 recognition probed with synthetic peptides, 1999, Biochem.
Biophys. Res. Commun., 263(3):691-695
24. Zhou, N., Z. Luo, J. Lou, X. Han, J. Hall and Z. Huang, Experimental and
theoretical studies of CCR5 interaction with chemokine. 1999, Eur. J. Immunol.,
30(1): 164-173, (2000).
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25. Zhou, N., L. Paements, G. Froyen, A. Billiau and G. Opdenakker, Cloning and
expression in E. coli of a single-chain immunologlobulin variable fragment (ScFv)
that specifically inhibits the activity of human gelatinase B, 1997, FEBS Letters,
414(3): 562-566.
26. Zhou, N., P. Matthys, C. Polacek, P. Fiten, A. Sato, A. Billiau and G. Froyen, A
competitve RT-PCR method for the quantitative analysis of cytokine mRNAs in
mouse tissues. 1997, Cytokine, 9(3): 212-218.
27. Zhou, N., Y. Zhang, W. Jing, Z. Li and X. Wu, High-level expression of
HEPATITIS B surface antigen in Bm cells and Larvae and pupae of silkworm (
Bombyx mori ) with BmNPV expression vector. 1995, Chinese Journal of
Biotechnology, 11(3):211-216.
PRESENTATION
1. Distinct Kinetic and Spatial Patterns of PKC- and EGFR-Dependent Activation
of Extracellular Signal-Regulated Kinases 1 and 2 by Hydroxy-Carboxylic Acid
Receptor 2. 2009 Gordon Research Conference on Mechanism of Cell Signaling.
Jul. 31-Aug. 5, 2011, Bates College, Lewiston, ME.
2. Molecular Characterization of Internalization of the Human Nicotinic Acid
Receptor HM74a in HEK293. 2009 Gordon Research Conference on
Phosphorylation and G Protein Signaling Networks. June 7-12, 2009
University of New England, Biddeford, ME
3. The N-Terminal Domain of APJ, a CNS-based Co-receptor for HIV-1, Is
Essential for its Novel Receptor Function and Co-receptor Activity. 11th
Retrovirus Conference. Moscone West, San Francisco CA, 2004.
4. Internalization and recycling of APJ, an HIV-1 Co-receptor, in transfected cells
and human neurons, the 5th Annual Meeting of the American Society of Gene
Therapy, Boston, MA, 2002.
5. Experimental and theoretical studies of CXC4 interaction with chemokine and
HIV-1. 9th Retrovirus Conference. Seattle, WA, 2002.
6. Synthetic Peptides Derived from the N-Terminus of the Viral Chemokine vMIPII Display Distinct Interactions with CXCR4. Seventh Annual Conference of
International Cytokine Society. Hilton Head Island, SC, 1999.
7. Monoclonal Antibodies Against Gelatinase B: Possible Diagnostic and
Therapeutic Applications. Annual Symposium of European Biochemistry
Society: Matrix Metalloproteinases in Tissue Remodeling. London, 1997.
8. Increased circulating IFN-gamma levels resulting from functional IFN-gamma
blockade are not due to increased IFN-gamma synthesis in the spleen. Annual
Meeting of the International Society for Interferon & Cytokine Research, San
Diego, CA, 1997.
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