农学院 2009 年 04 月共发表 SCI 论文 12 篇
1.
【篇名】Kinetic Analysis of Solid-State Reactions: A General Empirical Kinetic Model
【作者】Cai, J. M.,Liu, R. H.
【期刊】Ind. Eng. Chem. Res.,2009, 48(6)
【摘要】In this research note, we presented a general empirical kinetic model that
can fit any ideal kinetic model and even deviations produced by
heterogeneities in particle shapes. The algebraic expression of the general
empirical kinetic model is f(alpha) = alpha(m) (1 - q alpha)(n). The kinetic
model can be used for performing the kinetic analysis of experimental data
without previous assumptions about the form of the reaction kinetic model.
The empirical kinetic model has been evaluated from the theoretical and
experimental data.
2.
【篇 名】Effects of Temperature, pH, Agitation and Particles Stuffing Rate on
Fermentation of Sorghum Stalk Juice to Ethanol
【作者】Shen, F.,Liu, R.,Wang, T.
【期刊】Energy Sources Part A-Recovery Util. Environ. Eff.,2009, 31(8)
【摘要】The main factors, including temperature, agitation rate, pH, and particles
stuffing rate, were chosen in ethanol fermentation from stalk juice of sweet
sorghum using immobilized S. cerevisiae in shaking flasks in order to
determine suitable conditions. The results showed that a temperature of 34C,
agitation rate of 150-200 r min-1, pH of 4.5, and particles stuffing rate
of 25% should be selected as the suitable condition. The results of
verification experiments at the selected condition demonstrated that the
ethanol yield, the CO2 weight loss rate, and fermentation time were 95.15%,
0.508 g h-1, and 6 h in the shaking flasks, respectively. Meanwhile, the
ethanol yield and the fermentation time were 96.72% and 10 h in the 5 L
bioreactor. As a result, it could be concluded that the determined condition
was suitable and reasonable for ethanol fermentation from by immobilized S.
cerevisiae.
3.
【篇名】Molecular Cloning and Characterisation of Alpha Subunit of H+-ATPase in
Lactobacillus casei Zhang
【作者】Chen, X.,Yang, M.,Sun, Z. H.,Liu, W. J.,Sun, T. S.,Meng, H.,Zhang, H. P.
【期刊】Czech. J. Food Sci.,2009, 27(1)
【摘要】The acid tolerance is an important property of Lactic acid bacteria as
potential probiotics. H+-ATPase is considered a key gene in several bacteria
with the ability of acid tolerance. We cloned and sequenced the full length
cDNA of alpha subunit of H+-ATPase gene in Lactobacillus casei Zhang, which
had been isolated from traditional home-made koumiss in Inner Mongolia of
China. The results showed that the respective cDNA sequence is composed of
1530 nucleotides and codes a putative protein including 509 amino acids. In
addition, we also reconstructed the phylogenic trees for H+-ATPase gene based
on amino acids sequences of diverse strains of Lactic acid bacteria.
4.
【篇名】Improved phytoremediation of oilseed rape (Brassica napus) by Trichoderma
mutant constructed by restriction enzyme-mediated integration (REMI) in
cadmium polluted soil
【作者】Wang, B.,Liu, L. X.,Gao, Y. D.,Chen, J.
【期刊】Chemosphere,2009, 74(10)
【摘要】In this study, oilseed rape (Brassica napus) was exploited in remediation
of Cd-contaminated soil in combination of Trichoderma koningii. To improve
its phytoextracting efficiency, restriction enzyme-mediated integration was
used to construct Trichoderma mutants with higher Cd resistance. Of 200
mutants, 10 mutants were shown with higher Cd tolerance and enhanced ability
of removing Cd from growth medium. In pot experiment, mutant P6 significantly
alleviated the negative impacts of Cd on oilseed rape growth, and improved
the Cd uptake ability of oilseed rape shoot in Cd contaminated soil (p < 0.05).
Based on the dry weight, the amounts of Cd in shoots of mutant P6 treated
oilseed rape were increased by 23% and 38% per pot compared with wild type
Trichoderma treatment; 53% and 107% against non-inoculated treatment,
respectively, at 20 and 50 mg Cd kg(-1) soil. The results suggested the
Trichoderma mutant-oilseed rape symbiosis system could be used in
remediation of soil contaminated with heavy metal Cd. (C) 2008 Elsevier Ltd.
All rights reserved.
5.
【篇名】The effects of drying following heat shock exposure of the desert moss
Syntrichia caninervis
【作者】Xu, S. J.,Liu, C. J.,Jiang, P. A.,Cai, W. M.,Wang, Y.
【期刊】Sci. Total Environ.,2009, 407(7)
【摘要】Desert mosses are components of biological soil crusts (BSCs) and their
ecological functions make assessment and protection of these mosses a
high-ranking management priority in desert regions. Drying is thought to be
useful for desert mosses surviving heat shock. In this study, we investigated
the role of drying by monitoring the responses of physiological characters
and asexual reproduction in the typical desert moss Syntrichia caninervis.
Heat significantly decreased chlorophyll content and weakened rapid recovery
of photochemical activity, and increased carotenoid content and membrane
permeability. Lethal temperatures significantly destroyed shoot
regeneration potential. In comparison with heat alone, drying significantly
increased protonema emergence time and depressed protonema emergence area.
Drying combined with heat accelerated water loss, followed by a decrease of
photosynthetic activity. Drying had different influences on membrane
permeability at different temperatures. When moss leaves were subjected to
a combined stress of drying and heat shock, photosynthesis was maintained
mainly due to the effects of drying on physiological activity although the
cellular morphological integrity was affected. Drying caused opposing
effects on moss physiological and reproductive characteristics. On the one
hand, drying caused a positive synergistic effect with heat shock when the
temperature was below 40 degrees C. On the other hand, drying showed
antagonism with heat shock when the moss was subjected to temperatures higher
than 40 degrees C. These findings may help in understanding the survival
mechanism of dessert mosses under heat shock stress which will be helpful
for the artificial reconstruction of BSCs. (C) 2008 Elsevier B.V. All rights
reserved.
6.
【篇名】Gibberellins and N-(2-chloro-4-pyridyl)-N '-phenylurea improve retention
force and reduce water core in pre-mature fruit of Japanese pear cv. Housui
【作者】Zhang, C. X.,Tanabe, K.,Lee, U.,Kang, S.,Tokunaga, T.
【期刊】Plant Growth Regul.,2009, 58(1)
【摘要】A study of the effect of a gibberellin A(3) + A(4) mixture (GAs) on pre-harvest
fruit drop of Japanese pear cv. Housui is reported. The GAs was applied alone
or in combination with N-(2-Chloro-4-pyridyl)-N'-phenylurea (CPPU) in a
lanolin paste to the abscission zone at the spur-end of the pedicel. The
results showed that the GAs and CPPU combination treatment increased the
pedicel-spur retention force relative to that of the untreated controls.
Histological studies showed an accelerated rate of cambium division and the
development of secondary xylem in the abscission zone near the spur-end of
pedicels treated with the GAs plus CPPU paste, which delayed the formation
of the abscission zone. Fruit quality (sugar, acid, firmness, color) was not
adversely affected by the application of GAs plus CPPU, although the
application of GAs alone promoted ripening. In contrast, the addition of CPPU
to the two GAs delayed fruit ripening, which was measured as ethylene efflux.
All treatments were without adverse effects on return bloom, measured as bud
size. The CPPU plus GAs treatment also suppressed the incidence of water core,
whereas the application of the GAs alone accelerated water core in this water
core susceptible pear variety.
7.
【篇名】Genomic Cloning and Characterization of a FIP-gsi Gene Encoding a Fungal
Immunomodulatory Protein from Ganoderma sinense Zhao et al.
(Aphyllophoromycetideae)
【作者】Zhou, X. W.,Xie, M. Q.,Hong, F.,Li, Q. Z.,Lin, J.
【期刊】Int J Med Mushrooms,2009, 11(1)
【摘要】A genomic DNA sequence encoding fungal immunomodulatory protein (FIP), the
FIP-gsi gene, was isolated from Ganoderma sinense (Ganodermataceae,
Basidiomycetes) using genomic walker technology. Analysis of 1072-bp
segments revealed that the gene contained a 501-bp 5'-flanking region, a
333-bp open read frame (ORF), and a 238-bp 3'-flanking region. There is one
putative TATA box and one possible CAAT box lieing in the 5'-flanking region.
The ORF encodes a 12.4-kDa precursor polypeptide. One intron was found in
the 5'-flanking region. The deduced amino acid sequence of FIP-gsi shares
high similarity with other FlPs, for example, G. lucidum (FIP-glu), G.
japonicum (FIP-gja), G. tsugae (FIP-gts), Flammulina velutipes (FIP-fve),
and Volvariella volvacea (FIP-vvo), which were found to be 99%, 86%, 86%,
61%, and 56% identical, respectively. The cloning of the genomic DNA sequence
is an important foundation for further study of its structure, expression,
and regulatory mechanisms.
8.
【篇名】Expression of thymosin alpha I concatemer in transgenic tomato (Solanum
lycopersicum) fruits
【作者】Chen, Y. H.,Wang, A. X.,Zhao, L. X.,Shen, G. A.,Cui, L. J.,Tang, K. X.
【期刊】Biotechnol. Appl. Biochem.,2009, 52(【摘要】T alpha I (thymosin alpha I),
an immune booster, plays an important role in the maturation, differentiation
and function of T-cells. It can also activate the production of cytokines
in dendritic cells. Tal is one of two thymosin proteins that have potential
future clinical applications. In order to express T alpha I protein in plants,
we designed and synthesized the T alpha I gene according to the plant codon
usage bias and created a novel 4 x T alpha I concatemer (four copies of the
T alpha I gene arranged end-to-end in tandem, designated 4 x T alpha I).
Subsequently, a plant binary expression vector, PG-pRD 12-4 x T alpha I, was
constructed
and
introduced
into
tomato
via
Agrobacterium
tumefaciens-mediated transformation. Through selection, 54 regenerated
tomato plants resistant to kanamycin were obtained, and four transgenic
tomato plants were further confirmed by PCR and Southern blotting. RT-PCR
(reverse transcription-PCR) analysis showed that the 4 x T alpha I gene was
transcribed specifically in tomato [Solanum lycopersicum (formerly
Lycopersicon esculentum)] fruits. ELISA analysis showed that the content of
the 4 x Tot I protein reached a maximum of 6.098 mu g/g fresh weight in mature
tomato fruit. Western-blot analysis further confirmed the expression of 4
x T alpha I protein in transgenic tomato fruits. The MTT
[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide] assay
showed the 4 x T alpha I protein derived from transgenic tomatoes exhibited
bioactivity that can stimulate the proliferation of mice splenic lymphocytes
in vitro, and the specific activity of T alpha I protein from the artificial
system was higher than that from the synthetic Escherichia coli system. This
study is the first to report successful expression of bioactive T alpha I
in plants, and also it will provide the basis for furl ther development of
the plant system to produce T alpha I.
9.
【 篇 名 】 Cordyceps fungi: natural products, pharmacological functions and
developmental products
【作者】Zhou, X. W.,Gong, Z. H.,Su, Y.,Lin, J.,Tang, K. X.
【期刊】J. Pharm. Pharmacol.,2009, 61(3)
【摘要】Objectives Parasitic Cordyceps fungi, such as Cordyceps sinensis, is it
parasitic complex of fungus and caterpillar, which has been used for
medicinal purposes for centuries particularly in China, Japan and other Asian
countries. This article gives a general idea of the latest developments in
C sinensis research, with regard to the active chemical components, the
pharmacological effects and the research and development of products in
recent years. Key findings The common names for preparations include
DongChongXiaCao, in Chinese, winter worm summer grass in English. It has many
bioactive components, such as 3'-deoxyadenosine, cordycepic acid and
Cordyceps polysaccharides. It is commonly used to replenish the kidney and
soothe the lung, and for the treatment, of Fatigue. It also can be used to
treat conditions such as night sweating, hyposexuality, hyperglycaemia,
hyperlipidaemia, asthenia after severe illness, respiratory disease, renal
dysfunction, renal failure, arrhythmias and other heart disease and liver
disease. Because of its rarity and outstanding curative effects, several
mycelia strains have been isolated from natural Cordyceps and manufactured
by fermentation technology, and are commonly sold as health food products.
In addition, some substitutes such as C. militaris and adulterants also have
been used; therefore, quality control of C. sinensis and its products is very
important to ensure their safety and efficacy. Summary Recent research
advances ill the Study of Cordyceps, including Cordyceps mushrooms, chemical
components, pharmacological functions and developmental products, has been
reviewed and discussed. Developing trends in the field have also been
appraised.
10.
【篇名】Bayesian mapping QTL for fruit and growth phenological traits in Lablab
purpureus (L.) Sweet
【作者】Yuan, J.,Yang, R. Q.,Wu, T. L.
【期刊】Afr J Biotechnol,2009, 8(2)
【摘要】In order to map the QTLs for the agronomic traits in Lablab purpureus (L.)
Sweet, we designed an F-2 population derived from lablab accession
('Meidou2012' and 'Nanhui23') for construction of genetic linkage map and
repeatedly measured three fruit traits (pod length, pod diameter and pod
fresh thickness) and three growth phenological traits (flowering time,
podding time and harvest maturity period) in F-2 population and F-3 lines
from two planting years. Bayesian model selection was used to analyze main
effect QTL, epistatic QTL and QTL by environment interactions (QEs) as well.
41 main effect QTLs (19 for fruit traits and 22 for growth phenological traits)
were identified on 11 linkage groups with small phenotypic variances. Thirty
nine pairs epistatic QTLs (19 for fruit traits and 20 for growth phenological
traits) were also found which accounted for large proportions of phenotypic
variations. And 11 QEs (8 for fruit traits and 3 for growth phenological
traits) were detected only in F-3 lines. Six QTLs and one QEs were found to
be pleiotropy. Pod length, pod diameter, pod fresh thickness, flowering time,
podding time and harvest maturity period each had stable QTLs, which could
be useful for breeding purposes and scientific reasoning.
11.
【篇名】Mesoscopic Simulation Study on the Aggregation Behavior between Lauryl
Alcohol Polyoxyethylene and Sodium Lauryl Sulfate
【作者】Zhang, S. X.,You, H.,Zhao, B.,Wang, Z. W.
【期刊】Acta Chim. Sin.,2009, 67(6)
【 摘 要 】 MesoDyn density functional simulation method was used to study the
interactions between lauryl alcohol polyoxyethylene (C12E10) and sodium
Lauryl sulfate (SDS). The micro dynamic process of aggregate formation and
the aggregate morphology were reported. The effect of shear to original phase
was studied by simulating with varied shear rate. Intuitively demonstrate
the gathering way for 20% C12E10/20% SDS system by two-dimensional slices
analysis. On this basis, using benzene and octanol as oil, the oil elimination
mechanism of 20% C12E10/20% SDS for these two representations were compared.
Simulation results showed that a strong synergetic effect was between
non-ionic surfactant C12E10 and anion surfactant SDS, as the accumulation
behavior occurred when the respective concentration was very low. Different
phases could be obtained at various shear rates for the same concentration,
which to a certain extent explained the difference between the experiment
and simulation. And it was found that the oil solubilization for the definite
system was different because of the oil different structures. In a word,
obtained results helped to enhance understanding of the synergistic
interaction and can provided certain theory instructions for the duplicated
system effective application.
12.
【 篇 名 】 Chemical constituents of the petroleum ether extract of Holotrichia
diomphalia larvae
【作者】Dong, Q. F.,Zhang, S. F.,Wang, Y. L.,Zhang, H. M.,Zhang, L.
【期刊】Chem. Nat. Compd.,2009, 45(1)