Figure S5. Venn diagrams illustrating unique and overlapping genes up- or down- regulated
more than 10-fold by indicated treatments, compared with expression in the respective tissues
of control (no chelator) at pH 5.8. Microarray analysis was performed using RNA extracted
from shoots and roots of seedlings in indicated treatments. Genes in each compartment are
listed in Supplemental Tables S5 to S8. In roots, 16 genes were up-regulated by both DMA and
EDTA, and 24 were down-regulated by both DMA and EDTA. Among the 16 upregulated
genes, NRT2.1 showed an approximately 100-fold increase in response to both DMA and
EDTA (Supplemental Table S7f). In roots, DMA treatment specifically up-regulated 85 genes
and down-regulated 121 genes; EDTA treatment specifically up-regulated 43 genes and
down-regulated 18 genes. In shoots, DMA specifically up-regulated 78 genes and
down-regulated 23 genes; EDTA specifically up-regulated 41 genes and down-regulated 51
genes. In shoots, 1 gene was upregulated by both DMA and EDTA, and 13 genes were
down-regulated by both DMA and EDTA.