Molecular weight markers for Gel filtration chromatography.Sigma product MW -GF-200.Contains 10mg cytochrome c, 15mg carbonic anhydrase, 50mg bovine serum albumen containing 0.3% dithiothreitol, 25mg alcoh ol dehydrogenase,15mg beta amylase containing 15% sodium
chloride, 4%glucose and 1% dithiothreitol , 50mg blue dextran.
165mg material per kit.
Harmful
R42-May cause sensitation by inhalation. Sigma recommend avoiding contact and inhalation.The full toxicological properties have n ot been fully
investigated.
25 or 50mM Tris buffer containing 0.1 or 1.0mM EDTA
500mls
N.S.H.
FPLC Purified inorganic pyrophosphatase enzyme samples. 0.5mls
NO RISK PHRASES AVAILABLE. Avoid inhalation,skin and eye contact. May be possible biological hazard see assessment 1683/G33.
Hazardous Substances Policy - Assessment
CHEMICAL HAZARD AND RISK ASSESSMENT
School of Biosciences
Name of
supervisor
Dr. T. W. Young
Assessment Number*
1798
Date of Assessment
03-05-2001
Signature
Assessor
C.Sampayo
Signature
Notes
A School COSHH form in Word is available on the School Server.
Available from the Health and Safety Unit.
Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.
Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.
Use a continuation sheet to expand any section of this form in hard copy version.
1
LOCATION OF THE WORK ACTIVITY
2
PERSONS WHO MAY BE AT RISK
List names where possible
Dr. T. W. Young, A. Wadeson, Ph.D. students
3
ACTIVITY ASSESSED
4
MATERIALS INVOLVED
NAME
Molecular weight
markers for Gel filtration
chromatography.Sigma
product MW-GF200.Contains 10mg
cytochrome c, 15mg
carbonic anhydrase,
50mg bovine serum
albumen containing 0.3%
dithiothreitol, 25mg
alcohol
dehydrogenase,15mg
beta amylase containing
15% sodium chloride,
4%glucose and 1%
dithiothreitol , 50mg blue
dextran.
25 or 50mM Tris buffer
containing 0.1 or 1.0mM
EDTA
FPLC Purified inorganic
pyrophosphatase enzyme
samples.
5
Laboratory G6
INTENDED USE**
The use of Sigma molecular weight markers for Gel filtration
chromatography.
AMOUNT
max/stored
Attach copies of data sheet(s)
HAZARD
165mg
material
per kit.
Harmful
500mls
N.S.H.
0.5mls
RISK PHRASES
R42-May cause sensitation
by inhalation. Sigma
recommend avoiding
contact and inhalation.The
full toxicological properties
have not been fully
investigated.
NO RISK PHRASES
AVAILABLE. Avoid
inhalation,skin and eye
contact. May be possible
biological hazard see
assessment 1683/G33.
HAZDAT NO***
BIOSCIENCESNO***
Give brief details and attach protocol/instructions
To be used as markers for molecular
Weight identification for gel filtration chromatography.
6
RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding
mothers especially at risk?
From the stock molecular weight reagents there is a risk of skin and eye contact and risk of inhalation of dust and
vapours which may also cause sensitisation especially by inhalation in the case of beta amylase. There is a risk
of skin and eye contact and inhalation of Aerosols from all the solutions when handling and pipetting. The
possible Biological risk from the FPLC purified inorganic pyrophosphatase enzyme solution is small but contact
Should be avoided .
7
CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
The risks associated with all the compounds and reagents are significant. Control measures should be taken
As detailed in section 8,together with Good Chemical Practice.
8
CONTROL MEASURES
Additional to Good Chemical Practice
In addition to Good Chemical Practice, care should be taken to minimise inhalation of substances and aerosols
especially where they contain potentially sensitising substances. Appropriate face masks should
be used . Gloves and goggles should be worn.
Waste containing molecular weight Markers, this includes such items as pipette tips, and contaminated
equipment and glassware should be rinsed and bagged prior to disposal or transffering to the normal wash up.
Check integrity of gloves and face mask and eye glasses before using.
9
INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
General Laboratory Practice
Only experienced laboratory personnel should be using these compounds and reagents.
10
MONITORING
Performance of control measures,
Personal exposure
Health Surveillance
11
WASTE DISPOSAL PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste Disposal.
Small quantities of solutions containing tris buffer is disposed of to the drain.
Chemicals can be disposed of according to the hazardous substances policy of the University.
12
REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
April 2006. 0r any change in protocol or amounts used.
13
EMERGENCY ACTION
TO CONTROL HAZARDS To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
Only adequately trained and experienced personnel should deal with emergency spills.
Solutions involved are small volumes .A chemical spill kit can be used.
TO PROTECT PERSONNEL Evacuation, protection for personnel involved in clean-up, Special First Aid
Only adequately trained and experienced personnel should deal with emergency spills.
Gloves and eye glasses and appropriate face mask should be worn to prevent contact with spills of the above
Solids and solutions.
TO RENDER SITE OF EMERGENCY SAFE
Wash spill site with soap and water and dry.
CONTACT
Clean-up/decontamination
DR YOUNG/A.WADESON.
PHONE
3523
10.10.00
*
**
***
Prefix T is used for Teaching Assessment Number.
Please include amount of chemicals used and how.
Hazdat No is the UNICOSHH datasheet report number.
Biosciences No is the Biosciences data sheet number.
UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.
BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.