Bauman Chapter 12 Answers to Critical Thinking Questions p. 355 In Chapter 4 (see p. 121), we discussed identifying microbes using dichotomous taxonomic keys. Design a key for the genera of protozoa discussed in this section. * Note that the chapter does not provide information on which to base a dichotomy for the three neurotoxic dinoflagellates. Yes Alveoli present? See next page Cilia? No Yes Photosynthetic? Apical band of cilia? Yes No Yes Midline band of cilia? Yes No No Didinium Neurotoxic? Paramecium Insect host? Yes Free-living? Yes No No Gymnodinium Gonyaulax or Pfiesteria * No Toxoplasma Balantidium Yes Vorticella Tick? Yes Babesia No Plasmodium Mitochondria present? Yes Photosynthetic? Yes No Flagella? No Yes No Free-living? 2 nuclei? Euglena Trypanosoma Yes Yes No No Entamoeba Shell? No Polar filament? Yes Yes No Insect host? Yes Insect host? Acanthamoeba Silica? Giardia No No No Nosemia Yes Yes Foraminifera Microsporidium Radiolaria Trychonympha Trichomonas p. 359 Fungi tend to reproduce sexually when nutrients are limited or other conditions are unfavorable, but reproduce asexually when conditions are more ideal. Why is this a successful strategy? Sexual reproduction increases genetic variation, increasing the odds of some progeny being well suited to new or challenging conditions (limited nutrients, etc.). When conditions are favorable and a genotype is successful, creating new genetic combinations is unnecessary and wasteful of energy. (Don’t mess with success.) p. 371 Why are cellular slime molds called “cellular”? Cellular slime molds have a single nucleus in each cell, and do not form a true syncytium or coenocyte. p. 366 Design a key for the genera of fungi discussed in this section. Reproduce sexually? Yes No Dimorphic? Zygospores? Yes Yes No No Histoplasma Rhizopus Ascospores? Trichophyton No Yes Monomorphic yeast? Monomorphic yeast? No Yes No Plant pathogen? Poisonous? Pathogenic? Yes Yes No No Yes Amanita a Claviceps Saccharomyces Yes Coccidioides Tuber No Agaricus Cortinellis Mycorrhizae? No Yes Penicillium Penicillin? No Neurospora p. 367 Since Prototheca is colorless, how do scientists know that it is really a green alga? Scientists know that Prototheca is a green alga based on the presence of genetic sequences common to the green algae. p. 369 Design a key for the genera of algae discussed in this section. * Note that the chapter does not provide information on which to base a dichotomy for the three neurotoxic dinoflagellates. Cell wall? Yes No Chrysolaminarin? Euglena No Yes Cellulose? Navicula No Yes Yes Agar? Laminarin? Yes No Macrocystis No Gelidium Chondrus Histones? Yes Yes Colorless? No Dinoflagellates (see comment above *) No Crosswalls? Prototheca Yes No Helical chloroplast? Yes Spirogyra Codium No Trebouxia p. 375 1. How are cysts of protozoa similar to bacterial endospores? How are they different? Cysts of protozoa are metabolically inactive and resistant to drying and some disinfectants, similar to bacterial endospores, but protozoal cysts are not as resistant as endospores to heat treatment and irradiation, and do not survive as long. Further, protozoan cysts are eukaryotic; whereas, bacterial endospores are prokaryotic. 2. The host of a home improvement show suggests periodically emptying a package of yeast into a drain leading to a septic tank. Explain why this would be beneficial. Saccharymyces (household yeast) is a metabolically versatile organism that can catabolize biomolecules completely in the presence of oxygen, and in the absence of oxygen can convert metabolic intermediates to useful substrates. Yeast can both participate in the breakdown of sewage wastes, and contribute to the establishment of a diverse microbial community that can effectively recycle the materials in the septic tank. 3. Why doesn’t penicillin act against any of the pathogens discussed in this chapter? Penicillin interferes with the synthesis of peptidoglycan cell walls, a structural feature of most bacteria that is not present in eukaryotes. 4. How can one distinguish between a filamentous fungus and a colorless alga? Fungi produce cell walls made of chitin, while algae do not have chitin. The fungi share genetic sequences with animals, while algae are related closely to plants. 5. Why do scientists as a group spend more time and money studying protozoa than they do studying algae? Protozoa are highly diverse in their metabolism, structures, etc., so they are very interesting to study. Some protozoa cause disease, while algae are largely beneficial and not harmful. More money is available to study organisms that cause disease (troublemakers are often more interesting). 6. Why are there more antibacterial drugs than antifungal drugs? Fungi are eukaryotes and have metabolisms similar to that of humans, whereas bacteria have more significant differences in structure and metabolism. As a consequence, it is much easier to find chemicals that are toxic to bacteria but not to humans than it is to find chemicals toxic to fungi but harmless to humans.