1 Dissolved Oxygen Variability in the Sangamon River in the Vicinity of the Decatur, Illinois Wastewater Treatment Plant Discharge Field Team Larry D. Arnold, Jeff Runyon, Bill Cunningham Dr. W.D. Hatfield Laboratories, Sanitary District of Decatur, 501 Dipper Lane, Decatur, IL 62522 Jared Harris Engineering Department, Sanitary District of Decatur, 501 Dipper Lane, Decatur, IL 62522 Laboratory Analysis Support Latishia Greve, Nancy Dudley Introduction The Sanitary District of Decatur is a municipal wastewater treatment plant providing service to the city of Decatur, Illinois and several of the surrounding smaller municipalities. The plant has a design average flow of 41.0 MGD and a design maximum flow of 125.0 MGD. The plant is capable of primary treatment for solids removal, secondary treatment using an activated sludge process to remove dissolved biodegradable organics, and has a seasonal (May through October) disinfection requirement which is achieved using sodium hypochlorite solution to disinfect and a sodium bisulfate solution for chlorine residual removal prior to discharge to the Sangamon River. The district also operates four Combined Sewer Overflow facilities for primary treatment of excess flow during periods of excessive rainfall and land applies it’s anaerobically treated biosolids after lagoon storage each fall. One of the major factors affecting the compliance limits established for the district by the Illinois EPA (IEPA) is the fact that the district treatment plant is located downstream from the Lake Decatur dam. This means that at times there is minimal or no flow in the Sangamon River upstream from the plant and the seven day once in ten year low flow (7Q10) is 0 cubic feet per second. This fact results in the district’s effluent being subject to Illinois water quality standards as opposed to Effluent standards whose concentration limits are substantially higher in most cases. Among pollutants present in the district’s effluent for which current treatment processes are inadequate to meet anticipated water quality standards in Illinois are total nitrogen and total phosphorus. The IEPA has identified that dissolved oxygen (DO) and total nitrogen are potential causes of impairment in Sangamon River segments downstream of the treatment plant that are on the 303 (d) list of impaired waters(1). The IEPA is also currently evaluating establishing standards for the nutrients phosphorus and nitrogen. In the next National Pollutant Discharge Elimination System (NPDES) permit update cycle, the IEPA will be adding a requirement to monitor these parameters on a monthly basis. They have already established DO limits which are currently present in the district’s NPDES permit. One of the issues arising during the IEPA evaluation of these nutrient limits was the effect of nutrients on DO levels in the receiving stream. The presence of excess nutrients can cause algal growth 2 which is reflected in DO “swings”. Over a diel (24-hour) cycle, supersaturated DO levels may occur during the sunny part of the day when photosynthesis, and consequently DO production, would be expected to be highest. Additionally, DO levels may occur in the early morning, which may be lower than water quality standards for protection of aquatic life, due to the uptake of DO at night by aquatic life. For determination of whether these conditions exist, continuous monitoring of the receiving stream for DO and temperature (upon which DO concentration is dependent) is essential. The district decided to be proactive and investigate whether such conditions exist in the Sangamon River within the areas of influence of its effluent, during a summer low river flow period when dissolved oxygen levels are at their lowest during any given calendar year. Materials and Methods 1) YSI 6920 V@ Sonde with optical DO, Chlorophyll, Conductivity, and Temperature sensors with EcoWatch software installed on Hewlett Packard Probook laptop computer. 2) Metal containment cage, heavy-duty cable, and padlocks for securing Sonde in river 3) Hip length waders 4) Appropriate sampling bottles for water quality analyses to be performed concurrent with start and finish of in-river monitoring period for following methods : a) DO by Method 4500-O C., Standard Methods for the Examination of Water and Wastewater, 19th ed. b) Total Suspended Solids by Method 2540 D, Standard Methods for the Examination of Water and Wastewater, 19th ed. c) pH by Method 4500H+B, Standard Methods for the Examination of Water and Wastewater, 19th ed. d) Metals by US EPA Method 200.7 e) Ammonia-Nitrogen by Method 4500-NH3 D, Standard Methods for the Examination of Water and Wastewater, 19th ed. f) Total Phosphorus by Method 4500-P C, Standard Methods for the Examination of Water and Wastewater, 19th ed. g) Conductivity by Method 2510 B, Standard Methods for the Examination of Water and Wastewater, 19th ed. h) Nitrate-Nitrogen by US EPA Method 300.0 i) Total Organic Carbon by Hach Method 10173 j) Temperature by lab grade thermometer Site Sampling Procedure Prior to departure for each selected sampling site, sample interval and duration parameters for the sonde were adjusted and set to begin at a predetermined time; typically 8:30 am, and to take readings at 30 minute intervals for a period of 7 days. Upon arrival at each site, and once the optimum location for the apparatus was determined, the water temperature and manual depth were measured, and the frame stakes put into position. The cage 3 was then mounted to the stakes and adjusted to the appropriate depth. In each case, this was 6 inches from the bed of the stream per IEPA recommendations. During the initial set-up, and in one case, retrieval of the apparatus, a member of the engineering department took GPS measurements for mapping purposes, and assistance in estimating flow rates. Just prior to placing the sonde in the cage, water samples were collected for additional lab analysis. The sonde was then carefully loaded in the cage (with the probes facing upstream), and the cage closed and secured with a long-shackle padlock. Additionally, a heavy-duty cable was looped around a nearby stationary object (usually a tree near the shore), and the other looped end was placed on the same padlock. Water samples were then taken back to the lab to begin analysis. During the 7 day sampling period, a member of the lab staff went out on a daily basis, to check the apparatus for obvious tampering, and to ensure there were no debris hang-ups. At the end of the sampling period the lab staff returned to the site, and began again, by taking water temperature and manual depth measurements. Within 5 minutes of the final sampling, interval additional water samples were again collected for lab analysis. After the final sampling interval, the sonde was removed and taken to shore so the data could be transferred to the lap-top computer. Data was checked to ensure no obvious problems, such as fouling of the probes. Once the data was satisfactorily transferred to the laptop computer, all equipment was gathered and taken back to the lab, where analysis of the samples was begun, equipment cleaned, and the sonde checked for calibration drift. 4 Site One Results: Site One : Sangamon River at Lincoln Homestead State Park Geographical location of sonde placement : Latitude: 39o 48’ 3.13" North Longitude: 89o 6’ 18.3" West Continuous Monitoring Conditions at start of period: Samplers: Bill Cunningham, Jeff Runyon, Jared Harris, Larry Arnold Placement site characteristics: run following upstream riffle at park pull-off, just prior to Sangamon River Bridge on Lincoln State Memorial Parkway 5 Sampling start time: 08:30 on July 28, 2011 with sampling interval of 30 minutes Sampling stop time: 08:30 on August 4, 2011 Stream depth placement: 2 feet 2 inches by manual measurement Sonde depth placement: 1 foot 8 inches by manual measurement Estimated river flow = 70 ft width x 1.45 ft average depth x ca 1 ft/sec flow = ca 100 cfs Weather Conditions at start: Cloudy, Humid, 80o F Sonde Calibration: Calibration was performed by technical staff at Field Environmental Instruments, Pittsburgh, PA and results follow: Instrument 6920 Serial Number 6920R3 Firmware Version 3.10 Calibration Date: 7/13/11 Probe Calibration: Parameter Temperature Sp Conductivity 0 mS/cm Sp Conductivity 1.413 mS/cm Dissolved Oxygen % saturation Chlorophyll 0.0 Chlorophyll 113.0 Result 22.98 0.001 1.413 98.0 0.0 113.0 Pass/Fail Pass Pass Pass Pass Pass Pass Start of run inaccuracy check: Parameter Initial Sonde reading Dissolved Oxygen, mg/L 6.12 Post run inaccuracy check: Concurrent Laboratory Result Relative % Difference 5.85 4.5 Parameter Concurrent Laboratory Result Relative % Difference 5.65 1.4 Dissolved Oxygen, mg/L Final Sonde reading 5.73 Chlorophyll was considered being performed on a semi-quantitative basis. An Instrument Detection Limit of 1.0 µg/L was obtained analyzing tap water over a period of time. This translates to a Method Detection Limit of 4.0 µg/L and a Limit of Quantitation of 10 µg/L. Criteria established by the Illinois EPA for the inaccuracy check was a relative % difference of less than 20% which was met. (3) 6 7 Calibration Drift: Parameter Dissolved Oxygen, mg/L Test Solution concentration 8.87 Sonde reading Relative % Difference 8.83 0.45 Criteria established by the Illinois EPA for the calibration drift check was an absolute difference of less than 0.4 mg/L for DO which was met. (3) Continuous Monitoring Results for Dissolved Oxygen: During this run, water temperature ranged from 27.7 to 32.0 degrees C with an average of 29.9. Chlorophyll ranged from 6.6 to 44 µg/L with an average of 20. Dissolved Oxygen ranged from 5.73 to 11.5 mg/L with an average of 8.22. Conductivity ranged from 2.663 to 2.946 mS/cm with an average of 2.801. 8 Daily Dissolved Oxygen Summary (Each 24 hour period started at 8:30 a.m. and the final reading taken at 8:00 a.m. the next day) Average Minimum Maximum D.O. Swing Period Start Date D.O. (mg/L) D.O. (mg/L) D.O. (mg/L) (mg/L) 7/28/11 8.52 6.12 11.17 5.05 7/29/11 8.36 6.53 10.73 4.20 7/30/11 8.27 6.49 10.44 3.95 7/31/11 8.52 6.30 11.24 4.94 8/01/11 8.45 5.88 11.54 5.66 8/02/11 7.86 5.85 10.65 4.80 8/03/11 7.57 5.73 10.00 4.27 Since the lowest water temperature occurring during this run was ca 28 degrees centigrade and the elevation of the area is around 570 feet above sea level, the solubility of oxygen in fresh water at that temperature would be around 7.7 mg/L (4). Therefore, super saturation was occurring during the photosynthetic periods of the day. Water Quality Results for Site One at sample start up and completion : Parameter Day One (7/28) Day Seven (8/4) Total Suspended Solids (mg/L) 27 26 Total Organic Carbon (mg/L) 6 7 Ammonia-Nitrogen (mg/L) 0.07 0.06 Nitrate-Nitrogen (mg/L) 15.2 15.6 Phosphate-Phosphorus (mg/L), total 9.48 11.6 pH, S.U. 7.85 7.85 Specific Conductance, mS/cm 2.34 2.70 Dissolved Oxygen, mg/L 5.85 5.65 Hardness, mg/L 348 372 Nickel (mg/L), Total 0.0140 0.0159 Zinc (mg/L), Total 0.0168 0.0176 Weather Conditions for Site One Monitoring Date 7/28/11 7/29/11 7/30/11 7/31/11 8/1/11 8/2/11 8/3/11 8/4/11 Maximum Temperature, o F 96 89 87 93 94 96 93 89 Minimum Temperature, o F 77 74 70 71 69 78 68 63 Precipitation, “ 0 T 0 0 0 0 0 0 9 Site Two Results : Site Two: Sangamon River at Rock Springs Center just upstream from Wyckle’s Road Bridge Geographical location of sonde placement: Latitude: 39o 48’ 03.137 " North Longitude: 89o06’ 18.325" West Continuous Monitoring Conditions at start of period: Samplers: Bill Cunningham, Jeff Runyon, Jared Harris, Larry Arnold, Lee Slider Placement site characteristics: run just upstream from Wyckle’s Road Bridge Sampling start time: 08:30 on August 11, 2011 with sampling interval of 30 minutes Sampling stop time: 08:30 on August 18, 2011 10 Stream depth placement: 2 feet 2 1/2 inches by manual measurement Sonde depth placement: 1 foot 8 1/2 inches by manual measurement Estimated river flow = 106 ft width x 2.1 ft average depth x ca 0.25 ft/sec flow = ca 56 cfs Weather Conditions at start: Sunny, Dry, 67o F Sonde Calibration: Calibration was performed by Sanitary District staff and results follow: Instrument 6920 Serial Number 6920R3 Firmware Version 3.10 Calibration Date: 8/10/11 Probe Calibration: Parameter Dissolved Oxygen mg/L Result 8.85 @ 20.0 o C Action Set to Winkler result obtained for air-saturated water Start of run inaccuracy check: Parameter Dissolved Oxygen, mg/L Initial Sonde reading 5.27 Concurrent Laboratory Result Relative % Difference 5.16 2.11 Post run inaccuracy check: Parameter Dissolved Oxygen, mg/L Final Sonde reading 4.47 Concurrent Laboratory Result Relative % Difference 5.00 11.2 Chlorophyll was considered being performed on a semi-quantitative basis. Criteria established by the Illinois EPA for the inaccuracy check was a relative % difference of less than 20% which was met for the parameter evaluated. (3) Calibration Drift: Parameter Dissolved Oxygen, mg/L Test Solution concentration 8.35 Sonde reading Relative % Difference 8.20 1.81 Criteria established by the Illinois EPA for the calibration drift check was an absolute difference of less than 0.4 mg/L for DO which was met. (3) 11 12 Continuous Monitoring Results for Dissolved Oxygen: During this run, water temperature ranged from 25.6 to 30.3 degrees C with an average of 27.9. Chlorophyll ranged from 3.8 to 108 µg/L with an average of 9.2. Dissolved Oxygen ranged from 4.25 to 8.41 mg/L with an average of 6.19. Conductivity ranged from 3.297 to 3.596 mS/cm with an average of 3.442. Daily Dissolved Oxygen Summary (Each 24 hour period started at 8:30 a.m. and the final reading taken at 8:00 a.m. the next day) Average Minimum Maximum D.O. Swing Period Start Date D.O. (mg/L) D.O. (mg/L) D.O. (mg/L) (mg/L) 8/11/11 6.27 5.08 8.00 2.92 8/12/11 6.25 5.03 8.12 3.09 8/13/11 6.03 4.82 7.81 2.99 8/14/11 6.15 4.95 8.10 3.15 8/15/11 6.26 4.98 8.41 3.43 8/16/11 6.23 4.87 8.36 3.49 8/17/11 6.13 4.25 8.21 3.96 13 Since the lowest water temperature occurring during this run was ca 26 degrees centigrade and the elevation of the area is 580 feet above sea level, the solubility of oxygen in fresh water at that temperature would be around 8.0 mg/L (4). Therefore, some slight super saturation appears to have occurred during the photosynthetic periods of the day. Water Quality Results for Site Two at sample start up and completion: Parameter Day One (8/11) Total Suspended Solids (mg/L) 7.0 Total Organic Carbon (mg/L) 13 Ammonia-Nitrogen (mg/L) 0.20 Nitrate-Nitrogen (mg/L) 19.0 Phosphate-Phosphorus (mg/L), total 13.7 pH, S.U. 8.04 Specific Conductance, mS/cm 2.98 Dissolved Oxygen, mg/L 5.16 Hardness, mg/L 367 Nickel (mg/L), Total 0.0200 Zinc (mg/L), Total 0.0277 Day Seven (8/18) 7.5 16 0.17 13.1 15.6 8.00 3.16 5.00 373 0.0208 0.0186 Weather Conditions for Site Two Monitoring Date 8/11/11 8/12/11 8/13/11 8/14/11 8/15/11 8/16/11 8/17/11 8/18/11 Maximum Temperature, o F 83 86 89 83 84 84 87 91 Minimum Temperature, o F 55 56 64 61 56 54 59 66 Precipitation, “ 0 T T 0 0 0 0 0 14 Site Three Results: Site Three: Sangamon River at 7th Ward Combined Sewer Overflow (upstream from treatment plant) Geographical location of sonde placement: Not Determined due to sonde enclosure being pulled form water between 5:30 pm and 6:00 pm, August 27. Since this point was not critical to this evaluation, it was decided to abort this site due to the vandalism potential. Partial results are presented below. Continuous Monitoring Conditions at start of period: Samplers: Bill Cunningham, Jeff Runyon, Larry Arnold Placement site characteristics: pool just upstream from CSO discharge point Sampling start time: 08:30 on August 25, 2011 with sampling interval of 30 minutes Sampling stop time: 09:30 on August 29, 2011 – last valid in water reading at 17:30 on August 27, 2011 Stream depth placement: 2 feet 7 inches by manual measurement Sonde depth placement: 2 foot 1 inches by manual measurement Estimated river flow = ca 0 cfs (no dam discharge occurring and no observable flow) Weather Conditions at start: Sunny, Dry, 60o F Because the engineering technician was not available on start up day, no gps readings were taken. The intent was to do them at completion time. However, this was not done due to the sonde being pulled out of the water prematurely. Instrument 6920 Serial Number 6920R3 Firmware Version 3.10 Calibration Date: 8/23/11 Probe Calibration: Parameter Dissolved Oxygen mg/L Result 8.95 @ 20.0 o C Start of run inaccuracy check: Parameter Initial Sonde reading Dissolved Oxygen, mg/L 3.52 Action Set to Winkler result obtained for air-saturated water Concurrent Laboratory Result Relative % Difference 4.10 15.2 15 Chlorophyll was considered being performed on a semi-quantitative basis. Criteria established by the Illinois EPA for the inaccuracy check was a relative % difference of less than 20% which was met for the parameter evaluated. (3) During this aborted run, water temperature ranged from 23.2 to 26.3 degrees C with an average of 24.7. Chlorophyll ranged from 11 to 28 µg/L with an average of 19. Dissolved Oxygen ranged from 2.60 to 6.45 mg/L with an average of 4.35. Conductivity ranged from 0.643 to 0.679 mS/cm with an average of 0.658. 16 Daily Dissolved Oxygen Summary (Each 24 hour period started at 8:30 a.m. and the final reading taken at 8:00 a.m. the next day) Average Minimum Maximum D.O. Swing Period Start Date D.O. (mg/L) D.O. (mg/L) D.O. (mg/L) (mg/L) 8/25/11 4.46 2.69 6.45 3.76 8/26/11 4.38 2.60 6.32 3.72 8/27/11 4.00 2.69 5.20 2.51 (8/27/11 data represents 9 hours monitoring before device was pulled from river) No super saturation occurred at this site. Water Quality Results for Site Three at sample start up and completion: Parameter Day One (8/25) Total Suspended Solids (mg/L) 26.5 Total Organic Carbon (mg/L) 8 Ammonia-Nitrogen (mg/L) 0.11 Nitrate-Nitrogen (mg/L) 0.242 Phosphate-Phosphorus (mg/L), total 5.90 pH, S.U. 7.91 Specific Conductance, mS/cm 6.41 Dissolved Oxygen, mg/L 4.10 Hardness, mg/L 331 Nickel (mg/L), Total 0.00286 Zinc (mg/L), Total 0.0141 Weather Conditions for Site Three Monitoring Date 8/25/11 8/26/11 8/27/11 Maximum Temperature, o F 87 88 90 Minimum Temperature, o F 63 57 63 Precipitation, “ 0 0 0 17 Site Two (Run 2) Results (corresponds to engineering site 3 on gps diagram) : Site Two, Run 2: Sangamon River at Rock Springs Center just upstream from Wyckle’s Road Bridge Geographical location of sonde placement: Latitude: 39o 49’ 27.652 " North Longitude: 89o01’ 40.208" West Continuous Monitoring Conditions at start of period: Samplers: Bill Cunningham, Jeff Runyon, and Larry Arnold Placement site characteristics: run just upstream from Wyckle’s Road Bridge Sampling start time: 08:30 on September 1, 2011 with sampling interval of 30 minutes Sampling stop time: 08:30 on September 8, 2011 Stream depth placement: 2 feet 8 inches by manual measurement Sonde depth placement: 2 foot 2 inches by manual measurement Estimated river flow = 109 ft width x 2.0 ft average depth x ca 0.25 ft/sec flow = ca 55 cfs Weather Conditions at start: Sunny, Dry, 80 o F Instrument 6920 Serial Number 6920R3 Firmware Version 3.10 Calibration Date: 8/23/11 Probe Calibration: Parameter Dissolved Oxygen mg/L Result 8.93 @ 20.0 o C Action Set to Winkler result obtained for air-saturated water Start of run inaccuracy check: Parameter Dissolved Oxygen, mg/L Initial Sonde reading 4.98 Concurrent Laboratory Result Relative % Difference 4.87 2.23 Post run inaccuracy check: Parameter Dissolved Oxygen, mg/L Final Sonde reading 4.92 Concurrent Laboratory Result Relative % Difference 5.38 8.93 18 Chlorophyll was considered being performed on a semi-quantitative basis. Criteria established by the Illinois EPA for the inaccuracy check was a relative % difference of less than 20% which was met for the parameter evaluated. (3) Calibration Drift: Parameter Dissolved Oxygen, mg/L Test Solution concentration 8.93 Sonde reading Relative % Difference 9.03 1.11 Chlorophyll was considered being performed on a semi-quantitative basis. Criteria established by the Illinois EPA for the inaccuracy check was a relative % difference of less than 20% which was met for the parameter evaluated. (3) During this run, water temperature ranged from 22.9 to 31.7 degrees C with an average of 27.2. Chlorophyll ranged from 4.0 to 171.5 µg/L with an average of 16.2. Dissolved Oxygen ranged from 4.44 to 9.06 mg/L with an average of 5.95. Conductivity ranged from 2.784 to 3.718 mS/cm with an average of 3.282. 19 20 Daily Dissolved Oxygen Summary (Each 24 hour period started at 8:30 a.m. and the final reading taken at 8:00 a.m. the next day) Average Minimum Maximum D.O. Swing Period Start Date D.O. (mg/L) D.O. (mg/L) D.O. (mg/L) (mg/L) 9/01/11 6.39 4.58 9.06 4.48 9/02/11 6.18 4.49 8.84 4.35 9/03/11 6.08 4.65 8.77 4.12 9/04/11 5.28 4.44 6.46 2.02 9/05/11 5.61 4.51 7.42 2.91 9/06/11 5.92 4.60 7.86 3.26 9/07/11 6.19 4.83 8.18 3.35 Since the lowest water temperature occurring during this run was ca 23 degrees centigrade and the elevation of the area is 580 feet above sea level, the solubility of oxygen in fresh water at that temperature would be around 8.4 mg/L (4). Therefore, some slight super saturation appears to have occurred during the photosynthetic periods of the day. Parameter Total Suspended Solids (mg/L) Total Organic Carbon (mg/L) Ammonia-Nitrogen (mg/L) Nitrate-Nitrogen (mg/L) Phosphate-Phosphorus (mg/L), total pH, S.U. Specific Conductance, mS/cm Dissolved Oxygen, mg/L Hardness, mg/L Nickel (mg/L), Total Zinc (mg/L), Total Day One (9/01) 13 17 0.24 17.4 16.9 8.11 3.230 4.87 369 0.0197 0.0197 Day Seven (9/08) 14 23 0.20 20.9 18.5 7.91 3.220 5.38 362 0.0194 0.0256 Weather Conditions for Site Two Run 2 Monitoring Date Maximum Temperature, o F Minimum Temperature, o F 9/01/11 100 76 9/02/11 101 71 9/03/11 98 67 9/04/11 79 62 9/05/11 73 52 9/06/11 73 47 9/07/11 76 50 9/08/11 78 52 Precipitation, “ 0 0 0.41 0 0 0 0 0.11 21 RESULTS DISCUSSION As written by Dr. JoAnn Burkholder who represents the Mississippi River Collaborative: “Some Illinois EPA personnel have suggested that as an evaluation method, Illinois waters could be considered to require protection from nutrient pollution only if they met all three of the following characteristics: #1) wide variation in dissolved oxygen (DO) concentrations (> 6 mg DO/L, referred to as DO “swings.”) over a diel (24-hour) cycle; #2) super saturation during the sunny part of the day, when algal photosynthesis would be expected to be highest; and #3) DO levels in early morning less than the standard to protect beneficial aquatic life (generally 5 mg/L); this condition is referred to as having a pre-dawn DO “sag.””(2). The specific Illinois water quality standard for Dissolved Oxygen as applies to the Sangamon River at the Sanitary District of Decatur discharge point is as follows: “Section 302.206 Dissolved Oxygen General use waters must maintain dissolved oxygen concentrations at or above the values contained in subsections (a), (b) and (c) of this Section. a) General use waters at all locations must maintain sufficient dissolved oxygen concentrations to prevent offensive conditions as required in Section 302.203 of this Part. Quiescent and isolated sectors of General Use waters including but not limited to wetlands, sloughs, backwaters and waters below the thermocline in lakes and reservoirs must be maintained at sufficient dissolved oxygen concentrations to support their natural ecological functions and resident aquatic communities. b) Except in those waters identified in Appendix D of this Part, the dissolved oxygen concentration in the main body of all streams, in the water above the thermocline of thermally stratified lakes and reservoirs, and in the entire water column of unstratified lakes and reservoirs must not be less than the following: 1) 2) During the period of March through July, A) 5.0 mg/L at any time; and B) 6.0 mg/L as a daily mean averaged over 7 days. During the period of August through February, A) 3.5 mg/L at any time; B) 4.0 mg/L as a daily minimum averaged over 7 days; and C) 5.5 mg/L as a daily mean averaged over 30 days. (5)” An evaluation of each site as related to the three criteria mentioned above using the actual Illinois D.O. water quality standard follows: 22 Site One (Lincoln Homestead Park downstream from district discharge): 1) D.O. swings were < 6 mg/L – criteria does not apply 2) Super saturation was occurring – criteria does apply 3) All D.O.’s > 5.73 mg/L and Average 7 day D.O. is 8.22 mg/L - criteria does not apply Site Two Run 1 (Rock Springs Center near Wyckle’s bridge downstream from district discharge): 1) D.O. swings were < 6 mg/L – criteria does not apply 2) Super saturation was occurring – criteria does apply 3) All D.O.’s >= 4.25 mg/L - criteria does not apply Site Two Run 2 (Rock Springs Center near Wyckle’s bridge downstream from district discharge): 1) D.O. swings were < 6 mg/L – criteria does not apply 2) Super saturation was occurring – criteria does apply 3) All D.O.’s >= 4.44 mg/L - criteria does not apply At the upstream site near 7th Ward CSO facility (during the abbreviated run), D.O. readings were obtained below the 3.5 mg/L at any time criteria, but of course, no 7 day determination can be made with the incomplete data set obtained. One of our criteria for this study was to perform the evaluation during a “worst case scenario” of low flow in the Sangamon River. During the study period of July 28, 2011 through September 8, 2011, the river flow upstream from the district discharge ranged from 0.15 to 4.1 cubic feet/second with a mean of 1.8. Plant effluent flows were at summer lows during most of this period and were generally between 20 and 30 million gallons per day. Rainfall during the period was minimal and cloudy days were the exception during the period. (1) NPDES Permit No. IL0028321 for Sanitary District of Decatur Main STP, Effective Date : July 1, 2007, Illinois EPA, Issue date : April 20,2007. (2) Comments on Potential Use of Diel Patterns in Dissolved Oxygen Concentrations to Assess Nutrient Impairment in Illinois Surface waters, Dr. JoAnn Burkholder, April 29, 2011. (3) Standard Operating Procedure for Continuous Monitoring of Water Quality, Illinois EPA, Bureau of Water Document Control Number 128, Version 1.4, May 2010. (4) Making Dissolved Oxygen Measurements, YSI Incorporated. (5) Environmental Regulations for the State of Illinois, Title 35 of the Illinois Administrative Code, Subtitle A: General Provisions, Subtitle C: Water Pollution, Chapter I: Pollution Control Board, Part 302: Water Quality Standards, http://www.ipcb.state.il.us/SLR/IPCBandIEPAEnvironmentalRegulations-Title35.asp