BIOGRAPHICAL SKETCH
Provide the following information for the Senior/key personnel and other significant contributors in the order listed on Form Page 2.
Follow this format for each person. DO NOT EXCEED FOUR PAGES.
NAME
POSITION TITLE
Margarida Maria Rodrigues Barroso
Assistant Professor
eRA COMMONS USER NAME: barrosm
Barrosm
EDUCATION/TRAINING
INSTITUTION AND LOCATION
DEGREE
MM/YY
FIELD OF STUDY
Faculdade de Ciencias, University of Lisbon,
Portugal
B.S./M.S.
1986
Biology
Faculdade de Ciencias, University of Lisbon,
Portugal
Ph.D.
1991
Genetics
Postdoc
1995
Cell Biology
Princeton University, Dept. of Molecular Biology
Positions and Honors
Employment / experience
9/03- Present
Assistant Professor, Center for Cardiovascular Sciences, Albany Medical Center (AMC),
Albany, NY
9/95 -8/03
Assistant Professor, Dep. of Biology, University of Virginia (UVA), Charlottesville, VA
7/94-8/95
Research Staff Member, Dept. of Molecular Biology, Princeton University, Princeton, NJ
1/91 -6/94
Postdoctoral Research Associate, Dept. of Molecular Biology, Princeton University, NJ
Professional services
Honors: Secretary-Treasurer, Chair of the Finance Committee, Histochemical Society Officer (2011-2014);
Councilor, Histochemical Society Officer (2008-2011).
Study sections: NIH Enabling Bioanalytical and Imaging Technologies Study Section (EBIT) (Jan 2011); NSF
Cellular Organization Grant Review Panel (Oct 2001 & 2009-2010); Confocal Microscopy and High-End
Instrumentation grants S10 Review Panel (NIH ZRG1-CB F) (2006); Light Microscopy Shared Instrumentation
NIH Study Section Meeting (ZRG1 CBD) (2005); Study Section Meeting for the NIH Director's Roadmap
Initiatives for Biomedical Science in the 21st Century "Technology Centers for Networks and Pathways" (2004
& 2005)
Reviewer: Ad-hoc grant reviewer for University Grants Committee (Hong-Kong), ETH Zurich Research
Commission (Switzerland) and Cancer Research (UK) (2007-present); Ad-hoc grant reviewer for NSF and
Wellcome Trust (UK) (2000-present); Reviewer for Biochemical Journal, Biochimica et Biophysica Acta,
Biotechniques, Biophysical Journal, Brain Research, Cell Biology and Biophysics, FEBS Letters, Histology &
Histopathology, Journal of Biomedical Optics, Journal of Controlled Release, Journal of Histochemistry &
Cytochemistry, Journal of Microscopy, Journal of Physical Chemistry, Journal of the Royal Soc. Interface,
Molecular Biology of the Cell, Molecular Endocrinoloy, Nature Methods, Optics Express and Spectroscopy
Letters (2001-present)
Imaging-related teaching: Teaching Faculty in the FRET Microscopy Workshop at the Keck Center for Cellular
Imaging (KCCI), UVA, Charlottesville, VA (2002-2011); Teaching faculty in the Histochemical Society’s
“Immunohistochemistry & Microscopy” Practical Course at the Marine Biological Laboratory, Woods Hole, MA
(2010-2011); Teaching faculty in the FEBS Advanced Practical and Lecture Course on ‘Microspectroscopy:
‘Probing Protein Dynamics and Interactions in Living Cells’ at the Wageningen Microspectroscopy Centre and
the Nijmegen Microscopic Imaging Centre, The Netherlands (2010); Teaching Faculty in the EMBO course on
“Light Microscopy in Living Cells” at the Institute Gulbenkian of Science, Oeiras, Portugal (2005 & 2009);
Participant in the "Principles and Applications of Immunocytochemistry" course, The Histochemical Society,
Experimental Biology Meeting (2008); Teaching faculty in the Biomedical Interdisciplinary Research Workshop,
AMC Summer Cross-Training Undergraduate Research Program for Math and Engineering Undergraduate
Majors, Albany (2005-2009); Workshop coordinator, Workshop on FRET Microscopy, KCCI, UVA (2003).
Consulting: Consultant for patent litigation issues in imaging technology and for the development of FRETbased assays in NIH grants, 2008-present
Other: Chair (Lecture session), FRET at 65: A Celebration of Forster International Symposium (March 2011);
Table Leader for the Career Discussion and Mentoring Roundtables sponsored by the ASCB Women in Cell
Biology Committee, 50th ASCB Annual Meeting, 2010; Chair (Poster session), Multiphoton Microscopy in the
Biomedical Sciences VI, SPIE BiOS International Symposium on Biomedical Optics, San Jose, CA (2006).
Patents pending
1. U.S. Patent Application Serial No 12/026,413 entitled "FRET-Based Assay For Screening Modulators Of
Receptor Cycling", 02/05/2008.
2. U.S. Patent Application Serial No. 11/769,036 entitled "Methods And Apparatuses For Analyzing Digital
Images To Automatically Select Regions Of Interest Thereof", 06/27/2007.
Professional affiliations
American Society of Cell Biology (ASCB); Biophysical Society; Histochemical Society; Sigma Xi The Scientific
Research Society
Selected Peer-reviewed Publications
1. Barroso, M. Quantum Dots in Cell Biology. J. Histochem. Cytochem., 59: 237-251; PMID: 21378278, 2011
2. Barroso, M., Sun, Y., Wallrabe, H. and Periasamy, A. Nanometer-scale measurements using FRET and
FLIM microscopy In Luminescence: the Instrumental Key to the Future of Nanotechnology, Ed. A. Gilmore,
Pan Stanford, 2011
3. Bisaillon, J.M., Motiani, R.M., Gonzalez-Cobos, J.C., Potier, M., Halligan, K.E., Alzawahra, W.F., Barroso,
M., Singer, H., Jourd’heuil, D. and Trebak, M. Essential Role for STIM1/Orai1-Mediated Calcium Influx in
PDGF2 Induced Smooth Muscle Migration. AJP- Cell Physiol., 298: C993-1005; PMID: 20107038, 2010
4. Mingle, L.A., Bonamy, G.M., Barroso, M., Liao, G. and Liu, G. LPA-Induced Mutually Exclusive Subcellular
Localization of Active RhoA and Arp2 mRNA revealed by sequential FRET and FISH. Histochem. Cell
Biol., 132:47-58; PMCID: PMC2753266, 2009
5. Periasamy, A., Wallrabe, H., Chen, Y. and Barroso, M. Quantitation of protein-protein interactions:
Confocal FRET microscopy. Methods in Cell Biology, 89:569-98; PMID: 19118691, 2008
6. McGrath, N. and Barroso, M. Quantum dots as FRET donors in cells. J. Biomed. Opt., 13: 031210; PMID:
18601534, 2008
7. Wallrabe, H., Bonamy, G., Periasamy, A. and Barroso, M. Receptor complexes co-transported via
polarized endocytic pathways form clusters with distinct organizations. Mol. Biol. Cell, 18: 2226-2243;
PMCID: PMC1877110, 2007
8. Wallrabe, H., Chen, Y., Periasamy, P. and Barroso, M. Issues in confocal microscopy for quantitative
FRET analysis. Microsc. Res. Tech., 69:196-206; PMID: 16538626, 2006
9. Andrade, J., Pearce, S.T., Zhao, H. and Barroso, M. Interactions between p22, glyceraldehyde-3phosphate-dehydrogenase and microtubules. Biochem. J., 384(Pt 2):327-336; PMCID: PMC1134116, 2004
10. Andrade, J., Zhao, H., Titus, B., Pearce, S.T., and Barroso, M. The EF-hand Ca2+-binding protein p22
plays a role in microtubule and ER organization and dynamics with distinct Ca2+-binding requirements. Mol.
Biol. Cell, 15: 481-496; PMCID: PMC329218, 2004
11. Wallrabe, H., Stanley, M., Periasamy, A. and Barroso, M. One- and two-photon fluorescence resonance
energy transfer microscopy to establish a clustered distribution of receptor-ligand complexes in endocytic
membranes. J. Biomed. Opt., 8: 339-346; PMID: 12880337, 2003
12. Wallrabe, H., Elangovan, M., Burchard, A., Periasamy, A and Barroso, M. Confocal fluorescence
resonance energy transfer (FRET) microscopy to measure clustering of receptor-ligand complexes in
endocytic membranes. Biophysical J., 85: 559-571; PMCID: PMC1303111, 2003
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13. Elangovan, M., Wallrabe, H., Day, R.N., Barroso, M. and Periasamy, A. Characterization of one- and twophoton fluorescence resonance energy transfer microscopy. Methods, 29: 58-73; PMID: 12543072, 2003
14. Timm, S., Titus, B., Bernd, K. and Barroso, M. The EF-hand Ca2+-binding protein p22 associates with
microtubules in an N-myristoylation-dependent manner. Mol. Biol. Cell 10: 3473-3488; PMCID:
PMC25618, 1999
15. Barroso, M., Bernd, K. K., DeWitt, N. D., Chang, A., Mills, K. and E. S. Sztul. A novel Ca2+-binding protein,
p22, is required for constitutive membrane traffic. J. Biol. Chem. 271: 10183-10187; PMID: 8626580, 1996
16. Barroso, M., Nelson, D. and E. S. Sztul. Transcytosis-associated protein (TAP/p115) is a general fusion
factor required for binding of vesicles to acceptor membrane. Proc. Natl. Acad. Sci. USA. 92: 527-531;
PMCID: PMC42774, 1995
17. Barroso, M. and Sztul, E. S. Basolateral to apical transcytosis in polarized cells is indirect and involves
BFA and trimeric G protein sensitive passage through the apical endosome. J. Cell Biol. 124: 83-100;
PMCID: PMC2119901, 1994
In vivo Animal imaging: Poster presentations, invited seminars and publications
18. Venugopal, V., Chen, J., Barroso, M., and Intes, X. NIR FRET optical tomography, in preparation to
Biophysical Journal, 2012
19. Venugopal, V., Barroso, M. and Intes, X. Whole-Body Small Animal Quantitative FRET Tomography.
Poster presentation at 2011 World Molecular Imaging Congress, San Diego, 09/2011
20. Barroso, M., Venugopal, V., Chen, J., and Intes, X. Receptor-mediated uptake of NIR transferrin using
fluorescence tomographic tomographic FRET 3D whole body imaging. Invited presentation at the 37th
Annual Northeast Bioengineering Conference (NEBEC), 04/2011
21. Venugopal, V., Chen, J., Barroso, M., and Intes, X. Detection and Quantitation of NIR FRET in vivo Using
Time-Resolved Optical Tomography. Poster presentation at the International Symposium, FRET at 65: A
Celebration of Professor Theodor Förster, W.M. Keck Center for Cellular Imaging, UVA, 03/2011
22. Venugopal, V., Chen, J., Barroso, M., and Intes, X. In vivo reconstruction of NIR FRET using full-field time
resolved optical tomography, Proc. SPIE: 7892, 2011. Invited presentation at the 2011 Photonics West,
Multimodal Biomedical Imaging VI, Session 4: Pre-clinical, 02/2011
23. FRET imaging of Transferrin transport: Poster presentations, invited seminars and publications
24. Anderson, K., Mason, A., Steere, A. and Barroso, M. “Probing Conformational Changes in Transferrin
During Endocytic Acidification”. Poster presentation at Histochemistry 2011, New Trends in Microscopy
and Immunohistochemistry, The Marine Biological Laboratory, Woods Hole, MA, 04/2011
25. “Receptor FRETing in live cells”. Invited presentation at the 10th Annual Workshop on FRET Microscopy W.
M. Keck Center for Cellular Imaging (KCCI) University of Virginia, Charlottesville, VA, 03/2011
26. “Quantum dots as FRET donors in cells”. Invited presentation at the 10th Annual Workshop on FRET
Microscopy W. M. Keck Center for Cellular Imaging (KCCI) University of Virginia, Charlottesville, VA,
03/2011
27. Anderson, K., Mason, A.B., Steere, A.N. and Barroso, M. Probing Conformational Changes in Transferrin
During Endocytic Acidification. Poster presentation at 50th American Society of Cell Biology Meeting,
Philadelphia, PA, 12/2010
28. “Probing pH-Dependent Conformational Changes in Transferrin using Automated FRET”. Invited
presentation at the Microspectroscopy: Probing Protein Dynamics and Interactions in Living Cells,
Microspectroscopy Centre of Wageningen's University (MSCWU) and the Nijmegen Centre for Molecular
Life Sciences (NCMLS), Netherlands, 09/2010
29. “Probing Conformational Changes in Transferrin during Endocytic Acidification”. Invited presentation at the
37th Northeast Regional Meeting of the American Chemical Society, Potsdam, NY, 05/2010
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