SAFE WORK PROCEDURE
LOCATION
Fort Richmond Collegiate
WRITTEN BY:
APPROVED BY:
Solution and Dilution LAB
DATE CREATED
Science Team
Oct. 18, 2012
LAST REVISION
new
PERSONAL PROTECTION EQUIPMENT (PPE)
Safety glasses or face shield must be worn
at all times in work areas.
Long and loose hair must be tied back
Rings and jewelry (long necklaces /
bracelets, etc.) must not be worn.
Close fitting/protective clothing must be
worn. Remove strings hanging from
pullovers/sweaters.
Appropriate footwear must be worn. Shoe
must be fully enclosed. No open toed
shoes.
HAZARDS PRESENT


Chemical spill
Cuts – broken glassware
ADDITIONAL
REQUIREMENTS
APPARATUS / MATERIALS
balance
wash bottle
funnel
stirring rod
10 mL pipet
250 mL beaker
CuSO4∙5H2O
scoopula
100 mL volumetric flask
eye dropper


WHMIS
Safe Chemical handling
training
SAFE WORK PROCEDURE
Part A:
1. Put on safety goggles.
2. Carefully add the required amount of CuSO4∙5H2O using a scoopula, to the beaker on the scale. Be sure not
to touch, inhale, or taste the copper (II) sulfate pentahydrate.
3. Slowly add 40-60 mL of distilled water to the CuSO4∙5H2O in the beaker. Stir the solution slowly with a clean
stirring rod until it has all dissolved. Be careful not to splash while stirring.
4. Using a clean funnel, slowly transfer the solution from the beaker into a clean 100 mL volumetric flask.
Wipe up any spills immediately. Use the wash bottle to rinse any solution from the stirring rod, the beaker,
and then the funnel into the volumetric flask.
5. Use an eye-dropper to carefully bring the bottom of the solution meniscus to the 100.0 mL mark on the
volumetric flask. While holding flask in hand, tightly put on the stopper.
6. Invert several times, being careful not to get solution on skin. Wash hands if anything gets on skin.
7. Make sure to clean up work space, including balance area.
Part B:
1. Carefully pour the 0.200 M CuSO4 from Part A into a clean, dry 250 mL beaker.
2. Use a 10.0 mL pipet to transfer 10.0 mL of the 0.200 M CuSO4 solution into a clean
100 mL volumetric flask. Hold the pipette, NOT the rubber bulb. DO NOT insert the pipette directly into
the volumetric flask.
3. Add distilled water to the 10.0 mL of CuSO4 solution in the volumetric flask until the bottom of the solution
meniscus reaches the 100.0 mL mark.
4. Stopper the volumetric flask and invert several times, being careful not to get solution on skin. Wash
hands if anything gets on skin.
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5. Walking, take the final solution to your teacher.
REGULATORY REQUIREMENTS


WS&H Act W210, Section 4, 5
Mb. Regulations 217/2006,
Part 16, (Machines / Tools & Robots) Sections 16.1-16.18)
Part 35, (WHMIS Application)
Part 36, (Chemical & Biological Substances Application)
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Lab: Preparation of a Standard solution
and Dilution of a Known Solution
Purpose:
A: To accurately prepare a solution of known concentration.
B: To subsequently dilute the solution prepared in Part A.
Prelab Exercise: Calculate the mass of copper (II) sulphate pentahydrate (CuSO4∙5H2O(s))
required to prepare 100.0mL of a 0.200 mol/L solution.
Apparatus:
balance
CuSO4∙5H2O
stirring rod
eye dropper
250 mL beaker
funnel
100 mL volumetric flask
wash bottle (distilled water)
scoopula
10 mL pipet
Procedure:
Part A:
1.
Place a clean, dry 250 mL beaker on the scale, reset the scale to zero, and add the
required amount of CuSO4∙5H2O as calculated in the prelab exercise.
2.
Add 40-60 mL of distilled water to the CuSO4∙5H2O in the beaker. Stir the solution with
a clean stirring rod until it has all dissolved.
3.
Using a clean funnel, transfer the solution from the beaker into a clean 100 mL
volumetric flask. Ust the wash bottle to rinse any solution from the stirring rod, the
beaker, and then the funnel into the volumetric flask.
4.
Use an eye-dropper to carefully bring the bottom of the solution meniscus to the
100.0 mL mark on the volumetric flask. Stopper the volumetric flask and invert several
times.
Part B:
Proceed with the following steps to dilute the CuSO4 solution from Part A.
5.
Pour the 0.200 M CuSO4 from Part A into a clean, dry 250 mL beaker.
6.
Use a 10.0 mL pipet to transfer 10.0 mL of the 0.200 M CuSO4 solution into a clean
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100 mL volumetric flask.
7.
Add distilled water to the 10.0 mL of CuSO4 solution in the volumetric flask until the
bottom of the solution meniscus reaches the 100.0 mL mark.
8.
Stopper the volumetric flask and invert several times.
9.
Take the final solution to your teacher who will check it for colour intensity and/or
conductivity against a set of standard solutions. (Be sure to have labelled your flask).
Observations:
Mass of copper (II) sulphate pentahydrate required: ________________
Calculate the concentration of the solution after the dilution.
Questions:
1.
What property of the copper (II) sulphate solution changes noticeably upon dilution?
2.
The original and diluted solution will react with zinc metal. Predict which solution would
react with zinc at a faster rate? Why?
3.
Which solution would contain a greater number of moles of solute, 8.00 mL of the
concentrated copper (II) sulphate, or 40.0 mL of the diluted solution?
Show
calculations.
4.
Can a 0.2000 mol/L solution be prepared using the equipment employed in this lab?
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