Safe Work MS#...
Nanodrop
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Safe Work Method Statement
Nanodrop
Safe Work MS NUMBER
RISK ASSESSMENT
NUMBER
RISK ASSESSMENT
Safe Work MS#
RA#
Medium
DATE CREATED
Employees Involved in Safe Work
MS Creation
REVIEW DATE
30-9-2014
Carlie Delaine
30-9-2019
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Personal Protective Equipment Required
Laboratory coat or gown
Protective gloves
Eye protection
Users must have received authorised trained and the training documented
You must have read and understood this SWMS, the associated Risk
Assessment and the user notes for this equipment
Hazards:
 See below
Before work Commences
All nanodrop spectrophotometer users must be trained by their supervisor or
other appointed person. This training must be documented
Safe Work MS#...
Basic Task Step(s)
Switch on spectrophotometer
and allow to initialize.
Nanodrop
Hazards
Electrical hazard
Chemical hazard
Under standard methods tab,
select ‘Nucleic acid’ or
protein
Biological hazard
Add 2ul Nuclease free water
(NFW) to pedestal to initialize
and click ‘OK’.
After initializing machine
select ‘Manual Sample ID
entry’ and enter sample ID.
Check the correct nucleic
acid selected under sample
type: either ‘DNA-50’ for DNA
samples or ‘RNA-40’ for RNA
samples.
Add 2ul of NFW to each
pedestal and click on the
button ‘Blank’.
Wipe pedestal clean with
kimwipe.
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Safe Procedures
Wear gloves, eye protection
and covered shoes at all
times.
Keep samples in the ice all
the time.
Aliquot around 10ul (typically
2ul for one detection) from
each sample to avoid
contamination of the
samples.
Prepare one eppendorf of
Nuclease free water (NFW)
for the purpose of cleaning
the Nanodrop before and
after use
Bring a range of 0.5-10ul (or
0.5-2.0ul) pipettes and a rack
of pipette tips
Clean nanodrop with NFW
properly before and after use
Pipette 2 ul of sample (in
duplicate) to pedestal and
click on the button ‘Measure’
Once completed
measurements click on the
button ‘show report’ and
right click on this table to
export to excel.
Supervisor approval
Name__________________________ Date__________________________
Signature_______________________