Problem Supplement: Gene Regulation
BIOL 212 Spring 2007
1. Predict whether β-galactosidase will be produced by the following E. coli strains
under the condition given. The diploid genotypes represent F' lac strains.
Genotype
Lactose present
Lactose absent
a. i+p+o+z+
b. i-p+o+z+
c. i+p+ocz+
d. i-p+ocz+
e. i-p+oczf. i+p+o+zg. F' i+p+ocz-/i+p+o+z+
h. F' i+p+o+z-/i+p+ocz+
i. F' i+p+o+z-/i-p+o+z+
j. F' i+p+o+z+/i-p+o+zk. F' i+p+ocz-/i-p+o+z+
l. F' i+p+o+z-/i-p+ocz+
m. F' i-p+o+z-/i-p+ocz+
n. F' i-p+ocz-/i-p+o+z+
2. Would the following genotypes for the E. coli lac region be non-inducible, inducible,
or constituitive for β-galactosidase production?
a. i-p+o+zb. i+p+oczc. F' i-p+o+z-/i+p+o+z+
d. F' i+p+ocz-/i+p+o+z+
3. Match the correct term below to the description. A particular term may be used more
than once.
Positive control
Negative control
Inducible
Non-inducible
Constituitive
Repressor
Inducer
RNA polymerase
Operator
Promoter
a. Phenotype with respect to synthesis of β-galactosidase for partial diploid
F' i+o+z+/i-ocz-
___________________
b. Phenotype of F' i+o+z-/i+ocz+
_____________________
c. Phenotype of F' i+o+z-/i-ocz-
_____________________
d. Regulatory molecule that binds to promoter so transcription occurs.
________________
e. Small molecule that binds to repressor. ___________________
f. Regulatory molecule that binds to operator region in DNA. __________________
g. Control of gene regulation by a mechanism that prevents expression of genes until
their products are needed.
______________________________