Inquiry Science 10
Name:
REPLICATION, TRANSCRIPTION, & TRANSLATION PRACTICE
REPLICATION of DNA
1. Use the DNA segments provided to obtain the complementary DNA strands.
ATTCGATGC
TACGGATCG
___________________
___________________
CAGTGACTT
___________________
2. Which nitrogen base would you NEVER use during replication? _____________
3. What enzyme unzips the DNA strand?______________________ What enzyme proofreads the
new DNA ?_____________________________
4. When, during the life of a cell, does DNA replication occur ? ____________________________
TRANSCRIPTION
5. Use the DNA segments (genes!) provided to obtain an mRNA message.
ACTGGATAC
ACGGATCGT
TGACAGCTA
___________________
___________________
____________________
6. Which nitrogen base would you never use during transcription? ________________________
7. Where in the cell does transcription occur? __________________________________________
TRANSLATION:
8. USE the DECODING WHEEL to determine
the amino acids coded for by the mRNA
sequence below.
AUGGCGGAUCAACACUUU
mRNA CODON
AUG
GCG
GAU
CAA
CAC
UUU
AMINO ACID
*start in the middle of the wheel and work outward
9. How many different amino acids are there?___________________________________
10. Which two mRNA codons correspond to the amino acid histidine? ___________________
11. How many different mRNA triplets correspond to Threonine? _______________ What are
these triplets? ____________________________
12. Which amino acid is coded for AUG? ___________________ What is special about this
amino acid? ___________________________________________________
13. Give the amino acid sequence for the following mRNA message:
mRNA: A U G C C A U G G C A U
Amino acid sequence:
________________ - _______________ - __________________ - _________________
14. Look at the mRNA message below:
Number each of the t-RNA/amino acid complexes to show the sequence of this mini-protein.
_____
_____
_____
_____
15. Give the amino acid sequence for this mini-protein:
_______________ - ________________ - _________________- ______________
16. What would the resulting protein be if the first “U” was deleted?
_______________ - ________________ - _________________- ______________
17. Where in the cell does translation occur? ______________________________
18. _____________________, _________________, and _____________________ are
specific examples of proteins.
19. What is the role of hormones, in general? Name 2 hormones.
20. What is the role of enzymes, in general? Name 2 enzymes.
21. Some people think that mutations result in horrible disorders or physical deformities. The
entertainment industry would have you think that mutations result in amazing superpowers.
Please comment on this.
20. Match the parts in the diagram with the
correct name from the list below.
__C___ RIBOSOME
_____ NUCLEUS
_____ MESSENGER RNA
_____ ANTICODON
_____ AMINO ACID
______ CODON
______ TRANSFER RNA
22. What will happen to “D” after it drops off its amino acid?
23. Where do your cells obtain their supply of amino acids with which to build proteins for your
body?
24. What, in your own words, is a GENE?