Bio 93 Quiz 5: Master Copy
Transcription & Translation (Green)
**Choose 1-2 Questions from Kim Green’s Section **
1) The genetic code is essentially the same for all organisms. From this, one can logically
assume which of the following?
A) A gene from an organism can theoretically be expressed by any other organism.
B) All organisms have experienced convergent evolution.
C) DNA was the first genetic material.
D) The same codons in different organisms translate into the different amino acids.
E) Different organisms have different numbers of different types of amino acids.
2) Which of the following nucleotide triplets best represents a codon?
A) a triplet separated spatially from other triplets
B) a triplet that has no corresponding amino acid
C) a triplet at the opposite end of tRNA from the attachment site of the amino acid
D) a triplet in the same reading frame as an upstream AUG
E) a sequence in tRNA at the 3' end
3) Transcription in eukaryotes requires which of the following in addition to RNA
polymerase?
A) the protein product of the promoter
B) start and stop codons
C) ribosomes and tRNA
D) several transcription factors (TFs)
E) aminoacyl synthetase
4) The TATA sequence is found only several nucleotides away from the start site of
transcription. This most probably relates to which of the following?
A) the number of hydrogen bonds between A and T in DNA
B) the triplet nature of the codon
C) the ability of this sequence to bind to the start site
D) the supercoiling of the DNA near the start site
E) the 3-D shape of a DNA molecule
5) Accuracy in the translation of mRNA into the primary structure of a polypeptide
depends on specificity in the
A) binding of ribosomes to mRNA.
B) shape of the A and P sites of ribosomes.
C) bonding of the anticodon to the codon.
D) attachment of amino acids to tRNAs.
E) bonding of the anticodon to the codon and the attachment of amino acids to tRNAs.
6) Which of the following is the first event to take place in translation in eukaryotes?
A) elongation of the polypeptide
B) base pairing of activated methionine-tRNA to AUG of the messenger RNA
C) binding of the larger ribosomal subunit to smaller ribosomal subunits
D) covalent bonding between the first two amino acids
E) the small subunit of the ribosome recognizes and attaches to the 5' cap of mRNA
ATP/Enzymes & Cellular Respiration (Wood)
**Choose 4-5 Questions from Marcelo Wood’s Section**
7) If you couple an exergonic reaction with an endergonic reaction such that the
negative and why?
It would be negative because that would indicate that the overall coupling of the reactions
would allow the endergonic reaction to proceed.
8) Describe what you know about a phosphorylated intermediate with regard to energy
level and stability.
High energy, low stability
9) Pick one of the following:
Where does oxidative phosphorylation occur? Name two regions (not just mitochondria).
intermembrane space, mitochondrial matrix.
Where do the light reactions of photosynthesis occur?
Thylakoid, thylakoid membrane and space is ok….
Where do the calvin cycle reactions occur?
Stroma
Where does glycolysis occur?
Cytosol
10) Pick one of the following:
Describe what a reducing agent does.
(see lecture notes for answer)
Describe what an oxidizing agent does.
(see lecture notes for answer)
11) Why does NADH represent ‘reducing power’?
NADH contains electrons at high energy levels, which are then transferred to other
molecules. The molecules that receive those electrons are reduced.
12) Pick one of the following:
The proton concentration in the intermembrane space is high or low in order to drive
chemiosmosis during oxidative phosphorylation?
High
The proton concentration in the matrix is high or low in order to drive chemiosmosis
during oxidative phosphorylation?
Low
**You should have about 6 questions for Quiz 5. You can add more if you like.**