New England Biolabs, Canada
1815 Ironstone Manor, Unit 6
Pickering, Ontario, Canada
L1W 3W9
Please email this completed from to : info@ca.neb.com
General Questions
1. Customer Name:
2. Catalog Number:
3. Assay Date printed on tube:
4. Lot Number printed on tube:
5. How long has the customer had the product?
6. At what temperature was the product stored?
7. Was the product aliquotted?
 Yes
 No
Sample Information
1. What cell type was used for the sample lysate?
 Cell Line
Name: __________
 Primary Cells
 Whole Tissue
 Other: __________
2. What is the sample species:
 Human
 Mouse
 Rat
 Other: __________
3. Please state the induction/drug treatment used: ____________________
4. Please state the concentration of the drug used: ____________________
5. Please state the time points of induction: _________________________
New England Biolabs is the exclusive Canadian distributor for
Cell Signaling Technologies, Inc and Finnzymes, Oy
Telephone: 1-800-387-1095
info@ca.neb.com
www.neb.ca
New England Biolabs, Canada
1815 Ironstone Manor, Unit 6
Pickering, Ontario, Canada
L1W 3W9
6. Please state the positive control used: ____________________________
7. Please select the lysis buffer used:
 SDS Sample Buffer
 CST Cell Lysis Buffer
 CST Chaps Buffer
 RIPA Buffer
 Other: ___________
8. What specific phosphatase and protease inhibitors, if any, were included in the lysis
buffer?
9. Were the samples sonicated?
 Yes
 No
10. Please select the amount of protein lysate
loaded per well:
 20 micrograms
 Other: ___________
11. Please state the date of lysate preparation: _____________
12. Please select the lysate storage temperature:
 4°C
 -20°C
 -80°C
 Other: ___________
13. Has this antibody previously worked on
these samples?
 Yes
 No
Protocol Information
1. Please state the % acrylamide in the gel: ___________
2. Please select the membrane used:
 Nitrocellulose
 PVDF
 Nylon
 Other: ___________
New England Biolabs is the exclusive Canadian distributor for
Cell Signaling Technologies, Inc and Finnzymes, Oy
Telephone: 1-800-387-1095
info@ca.neb.com
www.neb.ca
New England Biolabs, Canada
1815 Ironstone Manor, Unit 6
Pickering, Ontario, Canada
L1W 3W9
3. What is the pore size of the membrane being used for transfer?
4. What are the membrane transfer conditions? Please indicate the applied current and
duration (i.e. 200 mA for 1 hr), and the transfer buffer used:
5. Have the transfer or running buffers been re-used?
6. Has the blot been stripped to re-probe?
Blocking
1. Please state the components of the blocking buffer:__________________
_____________________________________________________________
2. Please state the blocking time: _____________
Primary Antibody Incubation
1. Please state the primary antibody source: ______________
2. Please state the primary antibody dilution: ______________
3. Please state the components of the primary antibody dilution buffer: ______
_____________________________________________________________
4. Please state the incubation time for the primary antibody: _____________
5. Please state the washing buffer and time after primary antibody incubation: ________
Secondary Antibody Incubation
1. Please state the secondary antibody source and supplier: ______________________
2. Please state the secondary antibody dilution: _____________________
3. Please state the components of the secondary antibody dilution buffer:
_____________________________________________________________________
New England Biolabs is the exclusive Canadian distributor for
Cell Signaling Technologies, Inc and Finnzymes, Oy
Telephone: 1-800-387-1095
info@ca.neb.com
www.neb.ca
New England Biolabs, Canada
1815 Ironstone Manor, Unit 6
Pickering, Ontario, Canada
L1W 3W9
4. Please state the incubation time for the secondary antibody: __________
5. Please state the washing buffer and time after seconday antibody incubation:
_____________________________________________________________________
Detection
1. Please select the detection system used:
 Horseradish Peroxidase (HRP)
 Alkaline Phosphatase (AP)
 Other: ___________________
2. Please state the supplier of the detection system: ____________________________
3. Please state the length of time for film exposure: ____________________________
4. How many times have you observed this result: _____________________________
5. Please describe the appearance of the exposed and developed film:
New England Biolabs is the exclusive Canadian distributor for
Cell Signaling Technologies, Inc and Finnzymes, Oy
Telephone: 1-800-387-1095
info@ca.neb.com
www.neb.ca