Backbone Dynamics of the Heparin Binding Domain of VEGF165
Studied by Nuclear Magnetic Resonance Spectroscopy
Sung-Ah Lee1, Seung-Pil Yang2, Chi-Bom Chae2 and Yangmee Kim1*
1
Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, IBST
Konkuk University, Seoul, 143-701, Korea
2
Institute of Biomedical Science and Technology, Konkuk University
Seoul, 143-701, Korea
Alzheimer’s disease (AD) is accompanied by the progressive deposition of βamyloid (Aβ) in both senile plaques and cerebral blood vessels, loss of central neurons,
and vessel damage. Cerebral hypoperfusion is one of the major clinical features in AD
and likely plays a critical role in its pathogenesis. In addition to its major roles in
angiogenesis, vascular endothelial growth factor (VEGF) has neurotrophic and
neuroprotective effects. Vascular endothelial growth factor (VEGF) interacts with Aβ
and is accumulated in the senile plaques of AD patients’ brains. It is known that Aβ
binds to heparin-binding domain (HBD) of VEGF165. Thus it seems that Aβ recognizes
unique structural features of HBD of VEGF165. Spin relaxation rates of 15N nuclei
provide sensitive probes of the dynamic behavior of proteins in solution. In order to
study of dynamical properties of HBD, R1, R2, and heteronuclear NOE experiments
have been performed and backbone dynamics of HBD in sodium acetate (pH 5.5) at
300K and 290K by NMR was investigated by model-free analysis. Model free analysis
showed that the residues near the disulfide bonds in HBD which are expected to be the
ligand binding sites show the greatest flexibility (lowest S2 values). Flexibility of HBD
shown in this study is essential for its function to interact with Aβ or its ligands.
Probe Designs for In Situ NMR Studies of Direct Methanol Fuel Cell
Oc Hee Han*, Kee Sung Han, Younkee Paik, Seung-Soo Kim, Seen Ae Chae
Daegu Center, Korea Basic Science Institute
Daegu, 702-701, Republic of Korea
For remote site locations, automobiles, and mobile electrical devices such as
mobile phones and laptops, fuel cells are an ideal primary energy conversion device in
several aspects: 1) chemical energy stored in hydrogen and several hydrocarbon fuels is
significantly higher than that found in battery materials; 2) fuel cells are
environmentally friendly; 3) fuel cells have much higher efficiency to use the chemical
energy than thermal process. In terms of fuel storage and handling, direct methanol fuel
cells (DMFCs) have advantages over hydrogen fuel cells although catalysts with higher
efficiency are required.
In this presentation, our probe designs for the in situ NMR studies of DMFCs
will be introduced and some of our recent results obtained with the probe as well as ex
situ NMR data of DMFCs will be discussed.
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