BIOC 455: Techniques in Biochemistry and Biotechnology
Course Syllabus: Spring 2011
Laboratory Schedule
An experiment highlighted in bold type indicates a pre-lab quiz is due for that exercise
prior to the lab period.
Date
Experiment
Report
Wed Jan 19
Thu Jan 20
Course introduction
Check-in and lab safety
Mon Jan 24
Tue Jan 25
Dipeptide Sequencing, Day 1
Wed Jan 26
Thu Jan 27
Dipeptide Sequencing, Day 2
Mon Jan 31
Tue Feb 1
Dipeptide Sequencing, Day 3
Photometry
Wed Feb 2
Thu Feb 3
Problem Solving Session: 2:00-4:00 pm
Section A: 321 Greg Hall; Section B: 304 Noyes
Mon Feb 7
Tue Feb 8
-galactosidase,
Wed Feb 9
Thu Feb 10
-galactosidase,
Day 2
Mon Feb 14
Tue Feb 15
-galactosidase,
Day 3
Wed Feb 16
Thu Feb 17
Erythrocyte Membranes, Day 1
Mon Feb 21
Tue Feb 22
Erythrocyte Membranes, Day 2
Wed Feb 23
Thu Feb 24
LAB CANCELLED: Exam 1, 7:00-9:00 PM on Thu Feb 24 in 112 CA
Topics: Dipeptides, Photometry,  -galactosidase
Mon Feb 28
Tue Mar 1
Purification of GOT, Day 1
Wed Mar 2
Thu Mar 3
Purification of GOT, Day 2
Day 1
None
Notebook 1
Notebook 2
None
Notebook 3
Notebook 1
Notebook 2
1
BIOC 455: Techniques in Biochemistry and Biotechnology
Course Syllabus: Spring 2011
Laboratory Schedule
Date
Experiment
Mon Mar 7
Tue Mar 8
Purification of GOT, Day 3
Wed Mar 9
Thu Mar 10
Purification of GOT, Day 4
Mon Mar 14
Tue Mar 15
Purification of GOT, Day 5
GST Affinity Pull-down
Wed Mar 16
Thu Mar 17
SDS-PAGE of GOT & GST Samples
Sat Mar 19 thru
Sun Mar 27
Report
Notebook 2
SPRING BREAK
Mon Mar 28
Tue Mar 29
In vitro Transcription/Translation, Day 1
Notebook 3
Wed Mar 30
Thu Mar 31
In vitro Transcription/Translation, Day 2
Mon Apr 4
Tue Apr 5
Problem Solving Session: 2:00-4:00 pm
Both Sections: 304 Noyes Lab
Wed Apr 6
Thu Apr 7
LAB CANCELLED: Exam 2, 7:00-9:00 PM on Thu Apr 7 in 112 CA
Topics: Membranes, GOT, GST Pull-down
Mon Apr 11
Tue Apr 12
DNA Gel Shift Assay
PCR & Molecular Cloning, Day 1
Wed Apr 13
Thu Apr 14
PCR & Molecular Cloning, Day 2
Mon Apr 18
Tue Apr 19
PCR & Molecular Cloning, Day 3
Tue Apr 19
Wed Apr 20
PCR & Molecular Cloning, Extra Day
Wed Apr 20
Thu Apr 21
PCR & Molecular Cloning, Day 4
None
Notebook 1
Notebook 2
Laboratory Schedule
2
BIOC 455: Techniques in Biochemistry and Biotechnology
Course Syllabus: Spring 2011
Date
Experiment
Report
Mon Apr 25
Tue Apr 26
ELISA, Day 1
Problem Solving Session: 3:00-5:00 pm
Both Sections: 304 Noyes Lab
Wed Apr 27
Thu Apr 28
ELISA, Day 2
Mon May 2
Tue May 3
ELISA, Day 3
Finals Week
Exam 3, Date and Time To Be Announced
Topics: Transcription/Translation; Gel Shift; PCR & Cloning; ELISA
Notebook 3
Course Assignment Schedule
Date
Assignment Due
Wed Jan 19
Thu Jan 20
None
Mon Jan 24
Tue Jan 25
Pre-lab quiz: Dipeptide Sequencing
Due by Noon
Wed Jan 26
Thu Jan 27
None
Mon Jan 31
Tue Feb 1
Pre-lab quiz: Photometry
Due by Noon
5
Wed Feb 2
Thu Feb 3
Quiz 1: Dipeptide Sequencing
Open: 8 am on Feb 2 Due: 8 pm on Feb 3
20
Mon Feb 7
Tue Feb 8
Pre-lab quiz: -galactosidase
Due by Noon
10
Lab Report: Dipeptide Sequencing
Due by 2:00 pm in lab
24
Homework 1: Dipeptide Sequencing; Photometry
Due by 1:00 pm in lecture
25
Quiz 2: Photometry
Open: 8 am on Feb 9
20
Wed Feb 9
Thu Feb 10
Mon Feb 14
Tue Feb 15
Lab Report: Photometry
Due by 2:00 pm in lab
Point Total
5
Due: 8 pm on Feb 10
34
3
BIOC 455: Techniques in Biochemistry and Biotechnology
Course Syllabus: Spring 2011
Date
Assignment Due
Point Total
Wed Feb 16
Thu Feb 17
Pre-lab quiz: Erythrocyte Membranes
Due by Noon
10
Quiz 3: -galactosidase
Open: 8 am on Feb 16
20
Due: 8 pm on Feb 17
Mon Feb 21
Tue Feb 22
Lab Report: -galactosidase
Due by 2:00 pm in lab
Thu Feb 24
Exam 1, 7:00-9:00 PM
Topics: Dipeptides, Photometry, -galactosidase
93
Course Assignment Schedule
Date
Assignment Due
Mon Feb 28
Tue Mar 1
Pre-lab quiz: Purification of GOT
Due by Noon
20
Lab Report: Erythrocyte Membranes
Due by 2:00 pm in lab
45
Pre-lab calculations: Purification of GOT, Day 2
Due by 2:00 pm in lab
20
Wed Mar 2
Thu Mar 3
Point Total
Quiz 4: Erythrocyte Membranes
Open: 8 am on Mar 2 Due: 8 pm on Mar 3
Mon Mar 7
Tue Mar 8
Homework 2: Erythrocyte Membranes; GOT
Due by 1:00 pm in lecture
25
Pre-lab calculations: Purification of GOT, Day 3
Due by 2:00 pm in lab
Wed Mar 9
Thu Mar 10
None
Mon Mar 14
Tue Mar 15
Pre-lab quiz: GST Affinity Pull-down & SDS-PAGE
Due by Noon
5
Wed Mar 16
Thu Mar 17
Pre-lab calculations: Purification of GOT, Day 6
Due by 2:00 pm in lab
20
Quiz 5: Purification of GOT
Open: 8 am on Mar 16 Due: 8 pm on Mar 17
Sat Mar 19 thru
Sun Mar 27
Mon Mar 28
Tue Mar 29
SPRING BREAK
Pre-lab quiz: In vitro Transcription/Translation
Due by Noon
10
4
BIOC 455: Techniques in Biochemistry and Biotechnology
Course Syllabus: Spring 2011
Date
Assignment Due
Wed Mar 30
Thu Mar 31
Lab Report: Purification of GOT
Due by 2:00 pm in lab
120
Lab Report: GST Affinity Pull-down
Due by 2:00 pm in lab
22
Mon Apr 4
Tue Apr 5
Point Total
None
Course Assignment Schedule
Date
Assignment Due
Thu Apr 7
Exam 2, 7:00-9:00 PM
Topics: Membranes, GOT, GST Pull-down
Mon Apr 11
Tue Apr 12
Pre-lab quiz: DNA Gel Shift Analysis
Due by Noon
5
Lab Report: In vitro Transcription/Translation
Due by 2:00 pm in lab
20
Homework 3: Transcription/Translation; Gel Shift
Due by 1:00 pm in lecture
25
Pre-lab quiz: PCR & Molecular Cloning
Due by Noon
15
Mon Apr 18
Tue Apr 19
Lab Report: DNA Gel Shift Analysis
Due by 2:00 pm in lab
27
Wed Apr 20
Thu Apr 21
Quiz 6: DNA Gel Shift Analysis
Open: 8 am on Apr 20 Due: 8 pm on Apr 21
20
Mon Apr 25
Tue Apr 26
Pre-lab quiz: ELISA
Due by Noon
10
Wed Apr 27
Thu Apr 28
Lab Report: PCR & Molecular Cloning
Due by 2:00 pm in lab
65
Mon May 2
Tue May 3
Determination of J1 titer: ELISA, Day 3
Due by 2:00 pm in lab
Wed Apr 13
Thu Apr 14
Fri May 6
Point Total
Homework 4: PCR & Molecular Cloning
Due by 1:00 pm in lecture
25
Quiz 7: ELISA
Open: 8 am on May 2
20
Due: 8 pm on May 3
Lab Report: ELISA
Due by 5:00 pm in Dr. Grabner’s office
50
5
BIOC 455: Techniques in Biochemistry and Biotechnology
Course Syllabus: Spring 2011
Date
Assignment Due
Point Total
Finals Week
Exam 3, Date and Time To Be Announced
Topics: Transcription/Translation; Gel Shift; PCR & Cloning; ELISA
6