Title: Characterization of Ipomoelin promoter from sweet potato
M.S. Student : Yu-Lin Li
Advisor: Dr. Shih-Tong Jeng
Abstract:
Ipomoelin(IPO), a wounding-inducible defense protein from sweet potato, can be
induced by wounding , MeJA and ethylene, and is good target to study plants defense
system. The IPO promoter was isolated by iPCR and tail PCR from sweet potato
(Ipomoea batatas cv. Tainung 57), and contains 1483 bp of the upstream region from
the translational start codon of IPO. Five different deletions in the IPO promoter
region were created in order to locate its MJ and ethylene-responsive regions. Theβ
-glucuronidase (GUS) reporter gene was fused to these promoter segments in the
sense direction on PBI221 vector, and the transient expression of those constructs
induced by MJ and ethylene in tobacco mesophyll protoplast were studied. Results
indicated that the MJ-respond region is from the -552 to -921 bp and the
ethylene-respond region is between the -1176 and -1438 bp form the start codon of
IPO protein. Also, the regulatory elements of MJ and ethylene in IPO promoter are
different.