Purification of antibodies by zeolite A
Y. C. Huang, Y. C. Yu and T. Y. Lee
Department of Chemical Engineering, National Tsing Hua University,
Hsinchu, Taiwan, Republic of China
Received 20 December 1993;
accepted 24 October 1994.
Available online 28 December 1999.
Abstract
Zeolite A and its modified forms can be used to separate
immunoglobulin G from a mixture of plasma proteins and mouse
ascites fluid. The separation was achieved by adjusting the pH of
buffers according to the isoelectric points of proteins in the mixture.
Zeolite A with potassium cations (K-A) and its calcium phosphate
modified from (CaP-A) performed better than those with sodium,
ammonium cations, and dealuminated zeolite X, respectively.
Antibody fractionation eluted from zeolite A columns showed high
activity and purity, which were verified by sodium dodecyl sulfatepolyacrylamide gel electrophoresis and enzyme-linked
immunosorbent assay. A summary of the physical characteristics of
zeolite A in comparison with conventional materials is presented.
Zeolite is a promising new material as an ion exchanger or column
packing support for large-scale bioseparation.
Author Keywords: Antibodies; purification; zeolite; ion-exchange