Wolves, coyotes, and hybrids, oh my! And I'm not talking about werewolves. Hybridization is the interbreeding of two different species making fertile offspring. It can occur naturally in many plant, insect, fish, bird, and other organisms. It is thought to be an important aspect of evolution. However, hybridization can also be a big cause of endangerment to many species. (Fredrickson & Hedrick, 2005) If extensive, hybridization can threaten the survival of any animal. (Hailer & Leonard, 2009) It can lead to the extinction of one or both of the parental species when there are no genetically pure individuals. (Fredrickson & Hedrick, 2005) Hybridization with coyotes continues to threaten the recovery of endangered red wolves in the north. (Adams et al., 2006) Some say it might be the greatest biological threat to them. (Fredrickson & Hedrick, 2005) The question is: is there a way to locate and remove coyotes and hybrids so that the endangered wolves can multiply and prosper? Global warming isn't the only environmental threat that humans caused over the years. The spread of farming has thought to facilitate coyote expansion. As humans started industrializing and building new cities, coyotes and wolves started migrating. As they migrated, their paths intersected causing the coyotes and wolves to mingle with each other. As the different species hybridized, naturally the hybrids took characteristics from both parents. For example coyotes originating in the Great Plains, moved eastward over the past 90 years into a mix of forests and fields well suited for the deer and small prey these animals thrive on. Northeastern coyotes are not just larger but they also have wolflike features. These coyotes have wider mouths, with bigger skulls where chewing muscles attach, giving them a stronger bite for killing bigger prey. They eat more deer and they are comfortable in forests, much like wolves. This has allowed the hybrid coyotes to move more rapidly and capture deer, where as normal coyotes would eat smaller animals (Kays et al., 2009). Hybridization is making these hybrid coyotes stronger, quicker and larger but has sent the wolves straight to the endangered animals list. If reintroduced populations of wolves are to be self sufficient, it’s population size must increase. If the population remains this small, the individual wolves will be unable to find a wolf to mate with and may have to find a mate in a different species or may stop breeding altogether (Hailer & Leonard, 2009). For species already threatened by hybridization, increasing population numbers will not work if there is nothing preventing hybridization in the future. In these cases, preventing or stopping contact between the hybridizing species is the only course of action likely to prevent their loss. Sterilization can also be an effective, but short-term way to lessen the likelihood of red wolf and coyote interbreeding (Fredrickson & Hedrick, 2005). The key to preventing hybridization in populations of endangered species is the detection and removal of hybrids. This can be challenging for species that are wide-ranging and difficult to capture. Noninvasive genetic sampling, by collecting faecal DNA (also known as poop), is a promising alternative for locating hybrids within a population. Non-invasive techniques are beneficial because individuals do not need to be captured and sampling can occur over a large area. When this is combined with GIS technology (geographic information system) exact locations of hybrids can be found, permitting an easier approach to hybrid removal. This has been used to detect hybrids of endangered red wolves and coyotes (Adams et al, 2007). Hybridization occurs all over the world in all organisms. In some cases, like those of plants and in this case of the coyotes, hybridization can be a great factor of evolution. In other cases, like those similar to the red wolves, hybridization can lead to endangerment or even extinction. Efforts are being made to find ways for determining when hybridization is becoming a problem and how to stop it from becoming extensive to a point where it can become dangerous to one or both of the parental species. The collection of faecies is a way to obtain DNA without having to capture any animals. It also allows for a large range of sampling. This in addition to GIS technology will make identifying hybrids more efficient. New ways of preventing any more hybridization is still being researched and will continue to be researched until it is no longer a problem.