The Feinstein Institute for Medical
Research
Feinstein Facility Policy
POLICY TITLE: Ethidium Bromide
Disposal Policy
POLICY # GR034
DEPARTMENT: Research
Prepared by: Research Administration
Effective Date:
Revisions:
September 2008
August 2008
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PURPOSE
The purpose of this policy is to detail the procedures to be followed when disposing Ethidium
Bromide.
POLICY
Ethidium bromide is commonly used in molecular biology laboratories. While it is not regulated
as hazardous waste, the mutagenic properties of this substance may present a hazard if greater
than trace quantities are poured down the drain untreated or placed in the trash.
SCOPE
This policy applies to all members of The Feinstein Institute for Medical Research (“The
Feinstein”) workforce including but not limited to employees, business associates, volunteers,
students, office staff, and other persons performing work for or at The Feinstein’s 350 and 225
Community Drive locations.
PROCEDURE
ETHIDIUM BROMIDE GELS
Trace amounts of ethidium bromide in gels should not pose a hazard. Gels containing:

Less than 0.1 % ethidium bromide: may be placed in regular trash.

More than or equal to 0.1 %: must be collected and dispose of as hazardous chemical
waste.
ETHIDIUM BROMIDE SOLUTIONS

Aqueous solutions containing less than 10 ug/ml ethidium bromide may be disposed of
down the drain.

Aqueous solutions containing more than 10 ug/ml ethidium bromide should be filtered or
deactivated either by charcoal filtration (Funnel Extractor kit or the Green Bag kit) or
chemical neutralization. Alternatively, ethidium bromide solutions may be collected and
disposed of as hazardous chemical waste.

Ethidium bromide solutions containing heavy metals, organics, cyanides, or sulfides
must be disposed of as hazardous chemical waste.
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OTHER WASTE
Items such as gloves, test tubes, chux, papers, etc., contaminated with trace quantities of
ethidium bromide may be disposed of in regular trash. Items grossly contaminated with
ethidium bromide should be placed in clear bags and handled as hazardous chemical
waste.
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