Sedimentation Sample Preparation Form
Please fill out this form as much as you can and either e-mail it to sfchase@unh.edu or
mail it to:
Center to Advance Molecular Interaction Science
University of New Hampshire
Rudman Hall 379
46 College Road
Durham, New Hampshire 03824
I. Contact Information:
Contact Name:
Address:
City:
State:
Zip code:
E-mail address:
Telephone:
II. Sample Information:
If you have less than 3 samples, leave the corresponding fields blank:
Sample 1
Brief Description of
Sample
Approx. Molecular
Weight
Detection system (Int.,
FDS, or Abs
and wavelength if
Abs)
Buffer components
and concentrations
OD of Sample vs.
Buffer
OD of Buffer vs.
Water
Sample 2
Sample 3
Partial Spec. Volume
(V-bar)
Density of Buffer rel.
to H2O at 20C
Viscosity of Buffer rel.
to H2O at 20C
Run Temperature
Storage conditions
(temperature)
Special Notes
(handling or anything
else we need to know)
Amino Acid Sequence, Sample 1:
Please write a description for the amino acid sequence of sample 1:
Please enter the amino acid sequence of sample 1 (single letter code):
Amino Acid Sequence, Sample 2:
Please enter a description for the amino acid sequence of sample 2:
Please enter the amino acid sequence of sample 2 (single letter code):
Amino Acid Sequence, Sample 3:
Please writer a description for the amino acid sequence of sample 3:
Please write the amino acid sequence of sample 3 (single letter code):
Please bold or check YES to indicate that you have read the Chemical Compatibility
Table :
Yes
No
I have read the Chemical Compatibility Table (below)and understand
that I am liable for any damages caused by failing to identify
damaging chemicals in my buffer system
Chemical Compatibility Table
Anodized
Aluminum
Charcoal/
Epoxy
Acids (aq):
S
S
acetic acid (5%)/ethanoic acid
Acids (aq):
S
S
acetic acid (60%)/ethanoic acid
Acids (aq):
S
S
acetic acid (glacial)/ethanoic acid
Acids (aq):
S
S
boric acid
Acids (aq):
S
U
chromic acid (10%)
Acids (aq):
S
S
citric acid/2-hydroxy-1,2,3propanetricarboxylic acid
Acids (aq):
U
S
hydrochloric acid (10%)
Acids (aq):
U
U
hydrochloric acid (50%)
Acids (aq):
S
S
iodoacetic acid/2-iodoethanoic acid
Acids (aq):
S
M
mercaptoacetic acid/2-mercaptoethanoic
acid
Acids (aq):
S
U
nitric acid (10%)
Acids (aq):
S
U
nitric acid (50%)
Acids (aq):
-
S
oleic acid/cis-9-octadecenoic acid
Acids (aq):
U
S
oxalic acid/ethanedioic acid
Acids (aq):
U
1
perchloric acid (70%)1
Acids (aq):
U
S
phosphoric acid mixture (10%)
Acids (aq):
U
S
phosphoric acid mixture (50%)
Acids (aq):
S
M
picric acid/2,4,6-trinitrophenol
Acids (aq):
-
-
saturated fatty acids (see stearic acid)
Acids (aq):
-
S
stearic acid/octadecanoic acid
Acids (aq):
U
S
sulfosalicylic acid/3-carboxy-4hydroxybenzenesulfonic acid
Acids (aq):
U
U
sulfuric acid (10%)
Acids (aq):
U
U
sulfuric acid (50%)
Acids (aq):
-
-
thioglycolic acid (see mercaptoacetic acid)
Acids (aq):
U
S
trichloroacetic acid/trichloroethanoic acid
Acids (aq):
-
-
unsaturated fatty acids (sea oleic acid)
Chemical
Group:
Chemical
Anodized
Description:
Charcoal/ Epoxy
Description:
Group:
Aluminum
Bases (aq):
U
S
ammonium hydroxide (10%)
Bases (aq):
U
U
ammonium hydroxide (28%)
Bases (aq):
S
U
aniline/benzenamine
Bases (aq):
U
S
potassium hydroxide (5%)
Bases (aq):
U
S
potassium hydroxide (45%)
Bases (aq):
S
U
pyridine (50%)/azabenzene
Bases (aq):
U
S
sodium hydroxide (1%)
Bases (aq):
Chemical
Group:
U
Anodized
Aluminum
S
Charcoal/
Epoxy
Salts (aq):
U
S
aluminum chloride
Salts (aq):
S
S
ammonium acetate/ammonium ethanoate
Salts (aq):
S
S
ammonium carbonate
Salts (aq):
-
S
ammonium phosphate
Salts (aq):
M
S
ammonium sulfate
Salts (aq):
U
S
barium salts
Salts (aq):
U
S
calcium chloride
Salts (aq):
U
S
guanidine hydrochloride/laminomethanamidine hydrochloride
Salts (aq):
S
S
magnesium chloride
Salts (aq):
S
S
nickel salts
Salts (aq):
S
S
potassium bromide
Salts (aq):
U
S
potassium carbonate
Salts (aq):
S
S
potassium chloride
Salts (aq):
S
S
potassium permanganate
Salts (aq):
S
S
silver nitrate
Salts (aq):
S
S
sodium borate
Salts (aq):
U
S
sodium carbonate
Salts (aq):
U
S
sodium chloride
Salts (aq):
S
S
sodium nitrate
Salts (aq):
S
S
sodium sulfate
Salts (aq):
S
S
sodium sulfite
Salts (aq):
U
S
zinc chloride
S
zinc sulfate
Anodized Charcoal/
S
Salts (aq):
Chemical Group:
sodium hydroxide (>l%)
Description:
Description:
Aluminum
Epoxy
Gradient Forming Materials (aq):
-
S
cesium acetate/cesium
ethanoate
Gradient Forming Materials (aq):
S
S
cesium bromide
Gradient Forming Materials (aq):
S
S
cesium chloride
Gradient Forming Materials (aq):
S
S
cesium formate/cesium
methanoate
Gradient Forming Materials (aq):
S
S
cesium iodide
Gradient Forming Materials (aq):
S
S
cesium sulfate
Gradient Forming Materials (aq):
S
S
dextran or dextran sulfate
Gradient Forming Materials (aq):
S
S
Ficoll-Paque
Gradient Forming Materials (aq):
S
S
glycerol/1,2,3-propanetriol
Gradient Forming Materials (aq):
S
S
metrizamide
Gradient Forming Materials (aq):
S
S
rubidium bromide
Gradient Forming Materials (aq):
S
S
rubidium chloride
Gradient Forming Materials (aq):
S
S
sodium bromide
Gradient Forming Materials (aq):
S
S
sodium iodide
Gradient Forming Materials (aq):
S
S
sucrose/beta-Dfructofuranosyl-alpha-Dglucopyranoside
Gradient Forming Materials (aq):
S
S
sucrose, alkaline/beta-Dfructofuranosyl-alpha-Dglucopyranoside
Gradient Forming Materials (aq):
Anodized
Charcoal/
Chemical
Aluminum
Epoxy
Group:
Description:
Solvents:
S
U
acetone/2-propanone3
Solvents:
S
M
acetonitrile/ethanenitrile3
Solvents:
S
U
benzene3
Solvents:
1
U
carbon tetrachloride/tetrachloromethane
Solvents:
1
S
chloroform/trichloromethane
Solvents:
S
U
cresol mixture/methylphenol
Solvents:
S
S
cyclohexane3
Solvents:
S
S
diethyl ether/ethoxyethane3
Solvents:
-
M
diethyl ketone/3-pentanone3
Solvents:
S
M
N,N-dimethyiformamide/N,Ndimethylmethanamide3
Solvents:
S
S
dimethyl sulfoxide/sulfinylbis(methane)
Solvents:
S
S
dioxane/1,4-dioxacyclohexane3
Solvents:
S
S
ethanol (50%)3
Solvents:
S
S
ethanol (95%)3
Solvents:
-
-
ether (see diethyl ether)
Solvents:
S
S
ethyl acetate/ethyl ethanoate3
Solvents:
S
S
ethylene glycol/ 1,2-ethanediol
Solvents:
S
S
hexane3
Solvents:
S
S
isopropyl alcohol/2-propanol3
Solvents:
S
S
kerosene3
Solvents:
S
S
methanol3
Solvents:
1
S
methylene chloride/dichloromethane
Solvents:
S
S
methyl ethyl ketone/2-butanone3
Solvents:
S
M
phenol (5%)
Solvents:
S
U
phenol (50%)
Solvents:
S
U
tetrahydrofuran3
Solvents:
S
S
toluene/methylbenzene3
Solvents:
S
S
water
Solvents:
Chemical
Group:
S
Anodized
Aluminum
S
Charcoal/
Epoxy
xylene mixture/dimethylbenzene3
Detergents:
U
S
Alconox
Detergents:
S
S
deoxycholate, sodium dodecyl sulfate, Triton
X-100
Detergents:
S
S
Haemo-Sol
Detergents:
S
S
Solution 555Tm (20%)
Detergents:
S
S
Zephiran chloride (1%)
Detergents:
-
-
dibutyl phthalate (see n-butyl phthalate)
Detergents:
S
S
diethyl pyrocarbonate/pyrocarbonic acid
diethyl ester
Detergents:
-
U
ethylene oxide vapor4/oxirane3
Detergents:
S
S
formaldehyde/methanal
Detergents:
-
-
formalin (40%) (see formaldehyde)
Detergents:
U
U
hydrogen peroxide (3%)
Detergents:
U
U
hydrogen peroxide (10%)1
Detergents:
S
M
2-mercaptoethanol3
Detergents:
S
S
oils (petroleum)
Description:
Detergents:
-
S
oils (other)
Detergents:
S
S
physiologic media (e.g., culture media, milk,
serum, urine)
Detergents:
S
M
sodium hypochlorite
Detergents:
S
S
Tris buffer (neutral
pH)/tris(hydroxymethyl)aminomethane
Detergents:
Chemical
Group:
S
Anodized
Aluminum
Other:
S
S
urea
Charcoal/
Epoxy
S
Description:
n-butyl phthalate4/dibutyl 1,2benzenedicarboxylate
Legend:
S
satisfactory resistance
M
marginal resistance
U
unsatisfactory resistance
1
explosion hazard due to possible material/chemical reaction under rotor failure
conditions
2
OK below 26º C only
3
Flammability hazard. Not recommended for use in any type of centrifuge because
vapors may be ignited by exposure to electrical contacts. Depending on the
centrifuge type, such exposure could occur either during normal centrifugation or
under failure conditions.
4
nonaqueous
-
Unknown