INW Form A
Academic Year 2010
International Workshop on
Protein Expression and Purification Strategies
(Unit/Department: Research Division)
1. Certification in Protein Expression and Purification Techniques.
2. Language of Instruction: …English…
3. Duration of Workshop: 5 days (from 13 Sep 2010 to 17 Sep 2010)
4. Number of Attendant: 40
5. Program Description:
Overview of recombinant protein technology, describing advantages/drawbacks of
different recombinant protein expression systems. Strategies for recombinant protein
purification will be exposed with also a paid attention on the non-protein
contamination issues.
This workshop will be designed to provide a solid
understanding of specific topics through classroom presentation and laboratory work.
6. Course Coordinator:
Dr. Alain Jacquet, PhD
Division of Allergy and Clinical Immunology,
Recombinant vaccine unit
Department of Medicine, Faculty of Medicine,
Chulalongkorn University
Email: Alain.J@chula.ac.th
Dr. Kanitha Patarakul, MD, PhD
Department of Microbiology, Faculty of Medicine,
Chulalongkorn University
Email: k_patarakul@yahoo.com
7. Supervisors:
Dr. Alain Jacquet, PhD.
8. Program Objectives:
Improvements in protein expression technology and the development of efficient
large-scale purification processes to generate safe and reproducible materials at
reasonable cost.
INW Form A
Academic Year 2010
9. Program Contents:
13 Sep 2010
8.30-9.30
9.30-10.00
10.00-11.00
11.00-12.00
12.00-13.00
13.00-14.00
14.00-16.00
14 Sep 2010
8.30-9.30
9.30-10.00
10.00-11.00
11.00-12.00
12.00-13.00
13.00-16.00
15 Sep 2010
8.30-9.30
9.30-10.00
10.00-11.00
11.00-12.00
12.00-13.00
13.00-16.00
16 Sep 2010
8.30-9.30
9.30-10.00
10.00-11.00
11.00-12.00
12.00-13.00
13.00-16.00
17 Sep 2010
8.30-9.30
9.30-10.00
10.00-11.00
11.00-12.00
12.00-13.00
13.00-14.00
14.00-16.00
Lecture : Expression systems in bacteria: gram negative and gram
positive system (Dr.Kanitha Patarakul)
Coffee Break
Lecture: Expression systems in yeast (Dr.Surapol Piboonpocanun)
Lecture: Expression systems in mammalian cells (Dr.Alain Jacquet)
Lunch Break
Lab Briefing (focused on bacteria cell culture)
Lab: Culture growth and IPTG Induction of recombinant E.coli
expression
Lecture: Tagged/fusion protein (Dr.Alain Jacquet)
Coffee Break
Lecture: Purification strategies (Dr.Alain Jacquet)
Lab Briefing (focused on protein purification instrumentation)
Lunch Break
Lab: Cell lysis and protein purification using IMAC
Lecture: Immunological detection of recombinant protein expression
:ELISA, Western, Immunoprecipitation, Immunofluroescence and flow
cytometry (Dr.Nattiya Hirankarn/Dr.Pokrath Hansasuta)
Coffee Break
Lab Briefing
Lab: SDS-PAGE
Lunch Break
Lab: Western blot
Lecture: Expression systems in insect cells: direct transfection and
baculovirus (Dr.Alain Jacquet)
Coffee Break
Lecture: Characteristics of contaminants: host-cell proteins, endotoxin,
mycoplasma (Dr.Alain Jacquet)
Lab Briefing (focused on animal cell culture facilities)
Lunch Break
Lab: Mammalian cell transfection and other manipulating cells
techniques e.g, cell trypsination, cell counting, tranfer into other flasks)
Lecture: Recombinant antibodies or protein production by viral vectors
Coffee Break (Dr.Tanapat Palaga)
Lab Briefing
Lab: Detect GFP expression by flow cytometry
Lunch Break
Lab: Detect GFP expression by immunoflorescent microscope
Course Summary and Evaluation
10. Enrollment Fee: 5,000 Baht
11. Accommodation: Attendants can choose to stay at the Faculty Dormitory. (Dormitory Fee:
500 Baht / Night)
INW Form A
Academic Year 2010
12. Travel Grant for International Attendants: 10 Grants
Travel Grant including:
 Travel Fee Support: Maximum 10,000 Baht
 Accommodation Support: Faculty Dormitory (Waive Dormitory Fee)
Deadline for candidates who want to apply for Travel Grant: 15 July 2010
13. Application Requirements:
a. A copy of the applicant’s Curriculum Vitae.
b. A valid passport from the home country and eligibility to obtain a visa to travel to
Thailand if selected
14. Application Process:
Please submit the application and supporting documents in PDF files via Email:
smedsuk@md.chula.ac.th
15. Period of Application: Until Maximum
16. Further Information Contact:
International Program Coordinator
Graduate Division, Faculty of Medicine
Chulalongkorn University, RAMA 4 Road,
Pathumwan, Bangkok, 10330
Phone: 66-2-256-4475
www.md.chula.ac.th
Email: smedsuk@md.chula.ac.th