CURRICULUM VITAE
Lynette B. Corbeil (nee Keur), DVM, PhD
Education:
1956-1957
1957-1962
Pre-Veterinary Studies
Veterinary Medicine
1962-1963
Biblical Studies
1963-1965
M.Sc. Program in Cytology
and Immunology
PhD Program in Immunology
and Pathogenic Bacteriology
NIH Postdoctoral Fellowship
Immunology
NIH Postdoctoral Fellowship
Infectious Diseases
1971-1974
1974-1975
1975-1978
University of British Columbia
Ontario Veterinary College
University of Toronto
Graduate School of Missions
Columbia Bible College
Ontario Veterinary College
University of Guelph
New York State Veterinary College
Cornell University
New York State Veterinary College
Cornell University
School of Medicine
University of California, San Diego
Experience:
1962-1963
1963-1965
1965-1966
1966-1967
1967-1971
1971-1974
1974-1975
1975-1978
1978-1979
1979-1982
1982-1985
1985-1987
1985-1986
1987-1996
Part-time Veterinary
Practice
Graduate Research Assistant
Assistant Professor of
Immunology
Research Associate
(Immunology)
Assistant Professor of
Biology
Graduate Research Assistant
Postdoctoral Fellow
Postdoctoral Fellow
Associate Professor of
Immunology
Associate Professor of
Pathology and Director of
Laboratory Animal Diagnostic
Laboratory
Associate Professor of
Veterinary Microbiology
Professor of Veterinary
Microbiology
Visiting Professor
Adjunct Professor of
Pathology
Magnolia Animal Hospital
Ontario Veterinary College
Ontario Veterinary College
Mason Research Institute
Southeastern Massachusetts
University
New York State Veterinary College
New York State Veterinary College
School of Medicine
University of California, San Diego
College of Veterinary Medicine
Kansas State University
School of Medicine
University of California, San Diego
Washington State University
Washington State University
University of California, San Diego
University of California, San Diego
1989-2002
Courtesy appointment
1996-Present Professor of Pathology
1999-2002
Interim Director
UC Veterinary Medical CenterSan Diego
2002- 2010 Co-Director, UC VMC-SD
Professor of Population Health
& Reproduction
Assoc. Dean for S. California
Comp. Med.
2010-Present Professor Emeritus
Population Health &
Reproduction
University of California, Davis
Dept. Pop. Health & Reprod.
University of California, San Diego
UC San Diego and UC Davis
UCSD and UC Davis
UC Davis
UC Davis, School of Veterinary Medicine
UC Davis, School of Veterinary Medicine
RESEARCH FOCUS
Since my research training with Dr. A. J. Winter at Cornell, my goal has been to define
host parasite relationships in infections of the reproductive tract. Initially, this involved studies
of Campylobacter fetus for which we defined kinetics and functions of isotypic antibody
responses in bovine cervicovaginal secretions, uterine secretions and serum. Also, bacterial
antigenic variation in the face of a mucosal immune response was elucidated (in collaboration
with Winter and Schurig) for the first time. More recently, my focus has shifted to Histophilus
somni. Since the organism causes infertility by an ascending route, abortion by a hematogenous
route and respiratory infection by a descending route, as well as septicemia, it allows
investigation of several different virulence and defense mechanisms. My research team began by
defining the natural infection and by reproducing the disease in the natural host (cattle). Then,
we studied the specificity and isotypes of protective antibody responses. We defined the roles of
IgG1 and IgG2 in protection as well as exploring the differences between the IgG2 allotypes
(IgG2A1 and A2 or IgG2a and IgG2b). Sometime ago, we showed that IgG2 binds to H. somni
nonspecifically via the Fc moiety. Strains which bound IgG2 were serum resistant, but some
strains from asymptomatic carriers were serum sensitive and lacked IgG2 binding ability. To
best define bacterial antigens and virulence factors, we constructed a genomic library, expressed
H. somni antigens in E. coli and characterized the recombinants. Now, we have focused on
genes which are expressed in serum resistant but not in 4 serum sensitive strains of H. somni. By
Southern blotting, we showed the entire coding sequence of a very large virulence factor
(Immunoglobulin binding Protein A – IbpA) is missing in the serum sensitive strains.
Sequencing the 12.2 kb gene revealed several domains and motifs related to pathogenicity.
Recombinant vaccines using 3 of these domains revealed protective subunits for mice and calves.
Studies of the most protective domain (IbpA DR2), in collaboration with Jack Dixon’s lab,
revealed a “Fic” motif which is cytotoxic. The most sensitive cell type was bovine alveolar
epithelial type 2 cell, the target cell in bovine respiratory disease. The mechanism was shown to
be adenylylation or Rho GTPases. In a parallel study we are investigating the
immunopathogenesis of combined BRSV and H. somni in collaboration with Laurel Gershwin at
UC Davis. We showed that the combined infection enhances IgE responses, clinical scores and
duration of pneumonia. Surprisingly the primary specificity of the IgE response differed from
that of IgG. Now we are investigating differences in epithelial cell gene expression after BRSV,
H. somni or BRSV and H. somni infection. Thus, the research projects begin with the whole
animal, progress to a cellular/molecular approach, and then determine the significance of the
molecular studies in the natural host.
A secondary focus involves bovine trichomoniasis. The project was chosen for several
reasons. Firstly, unlike H. somnus infection, the organism remains in the reproductive tract,
providing a straightforward local host-parasite relationship for study. Secondly, it is caused by a
protozoan with a single stage life cycle, which grows well in broth or soft agar. Thirdly, the
disease is of great economic significance in areas where natural breeding is practiced. For
example, approximately 15% of herds in California were infected in a randomized study.
Fourthly, it is the only naturally occurring animal model for human trichomoniasis. Our team
(including Bob BonDurant at UC Davis) started by determining antibody specificities and
isotypes which may be protective. This was done with bovine immune and convalescent sera as
well as with a panel of monoclonal antibodies (Mabs) prepared in our laboratory. When Mabs
were found which immobilized and agglutinated the organism, mediated complement killing and
prevented adherence to vaginal epithelial cells, we immunoaffinity purified the surface antigen
(TF1.17). Collaborations with BN Singh at SUNY Syacuse revealed that the antigen was a
Lipophosphoglycan/protein conjugate. Immunization/challenge studies with this antigen in
virgin heifers showed significantly faster clearance of T. foetus in vaccinates than in adjuvant
controls (p <0.002). The systemically vaccinated heifers not only demonstrated increases in
serum and vaginal IgG antibodies to T. foetus but also demonstrated an anamnestic vaginal IgA
response on challenge with T. foetus. We vaccinated animals with protocols to enrich for IgG or
IgA antibodies in genital secretions. One group had greatly enriched IgA antibodies to TF 1.17
antigen and a second group, greatly enriched specific IgG antibody levels in vaginal secretions.
Both groups cleared the infection faster than the controls, but were not different from one
another. Thus, genital IgG and IgA antibodies to the same trichomonad antigen were equally
protective. We showed that systemic priming with this antigen followed by either vaginal or
intranasal boosting stimulated genital IgA responses and resulted in early clearance of the
organism. Most recently, we found that IgE antibodies to TF1.17 antigen were associated with
mast cell degranulation, IgG1 antibody influx into genital secretions and clearance of the
parasite. We investigated T. foetus extracellular cysteine proteinase (CP8) as a virulence factor
demonstrating it to cleave bovine IgG1, IgG2, C3 and lactoferrin. The gene was shown to be
expressed by T. foetus isolated but not further cultured from the bovine uterus during infection.
We have also developed mouse models for T. foetus and T. vaginalis infection and for T. foetus
induced reproductive failure. Most recently we demonstrated that CP inhibitors reduce
cytotoxicity of T. foetus for bovine trophoblasts and decrease murine vaginal infection.
Therefore, the focus of T. foetus research is also host parasite relationships in the whole animal
and at the molecular level.
A third area of research addresses the role of bovine IgG2 in defense against pyogenic
infections. With H. somnus infection, we have reported several lines of evidence that suggest
IgG2 is key in defense. Since IgG2 is a better opsonin than IgG1, it should also be important in
defense against trichomonads, which are known to be killed by PMNs. Others have shown that
IgG2 is important in other pyogenic infections also (especially pneumonia and mastitis). For
example, Red Danish Milk cattle deficient in IgG2 have significantly more pyogenic infections
than normal controls. We showed that young calves develop IgG2b antibodies much later than
IgG2a. Then we investigated the functions of the two IgG2 allotypes in: a) resistance to
digestion by trichomonad or staphylococcal proteinases, b) binding to surface immunoglobulin
binding proteins of H. somni, Staphylococcus aureus, and Streptococcus spp., c) initiation of
the complement cascade. These studies showed that IgG2b is approximately twice as effective in
complement activation as IgG2a. A second series of experiments showed that IgG2b bound to H.
somnus IgBPs but IgG2a did not. Lastly, IgG2a was digested more rapidly by T. foetus
extracellular cysteine proteinase than IgGb. The hope is that further studies will enable
correlation of susceptibility of animals to pyogenic infection with their IgG2 allotype (IgG2a/a,
IgG2a/b or IgG2b/b). This would provide a genetic marker for disease susceptibility along with
understanding of the underlying mechanisms of susceptibility/resistance.
PUBLICATION LIST
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
Campbell JJR, Phelps RH, Keur LB: Dependence of oxidation-reduction potential of
milk on its vitamin C content. J Milk Food Technol 22:346- 347, 1959.
Campbell JJR, Keur LB: Role of xanthine oxidase in the reduction of resazurin by raw
milk. J Dairy Sci 44:425-429, 1961.
Corbeil LB: Differentiation of rhabdomyosarcoma and neonatal muscle cells in vitro.
Cancer 20:572-578, 1967.
Corbeil LB: Antigenicity of rhabdomyosarcomas induced by nickel sulfide (Ni3S2).
Cancer 21:184-189, 1968.
Corbeil LB: Observations on multinucleation of rhabdomyosarcoma and neonatal muscle
cells in vitro. Life Sciences 8:651-656, 1969.
Ingram DG, Corbeil LB, Malcomson ME: Antibodies to Escherichia coli in young
calves: K antigens. Am J Vet Res 31:71-79, 1970.
Schurig GD, Hall CE, Burda K, Corbeil LB, Duncan JR, Winter AJ: Persistent genital
tract infection with Vibrio fetus intestinalis associated with serotypic alteration of the
infecting strain. Am J Vet Res 34:1399-1403, 1973.
Schurig GD, Hall CE, Burda K, Corbeil LB, Duncan JR, Winter AJ: Infection patterns in
heifers following cervico-vaginal or intrauterine instillation of Campylobacter (Vibrio)
fetus veneralis. Cornell Vet 64:533-548, 1974.
Davies DH, Corbeil LB, Ward D, Duncan JR: A humoral suppressor of in vitro
lymphocyte transformation in cattle with Johne's disease. Proc Soc Exp Biol Med
145:1372-1377, 1974.
Corbeil LB, Schurig GD, Duncan JR, Corbeil RR, Winter AJ: Immunoglobulin classes
and biological functions of Campylobacter (Vibrio) fetus antibodies in serum and
cervicovaginal mucus. Infect Immunity 10:422-429, 1974.
Corbeil LB, Duncan JR, Schurig GGD, Hall CE, Winter AJ: Bovine venereal vibriosis:
Variations in immunoglobulin class of antibodies in genital secretions and serum. Infect
Immunity 10:1084-1090, 1974.
Corbeil LB, Schurig GGD, Bier PJ, Winter AJ: Bovine venereal vibriosis: Antigenic
variation of the bacterium during infection. Infect Immunity 11:240-244, 1975.
Schurig GGD, Hall CE, Corbeil LB, Duncan JR, Winter AJ: Bovine venereal vibriosis:
Cure of genital infection in females by systemic immunization. Infect Immunity
11:245-251, 1975.
Corbeil LB, Corbeil RR, Winter AJ: Bovine venereal vibriosis: Activities of
inflammatory cells in protective immunity. Am J Vet Res 36:403-406, 1975.
Duncan JR, Corbeil LB, Davies DH, Schultz RD, Whitlock RH: Persistent
papillomatosis associated with immunodeficiency. Cornell Vet 65:205-211, 1975.
McCoy EC, Doyle D, Burda K, Corbeil LB, Winter AJ: Superficial antigens of
Campylobacter (Vibrio) fetus: Characterization of an antiphagocytic component. Infect
17.
18.
19.
20.
21.
22.
23.
24.
25.
26.
27.
28.
29.
30.
31.
32.
33.
34.
35.
Immunity 11:517-525, 1975.
Corbeil LB, Shively JN, Duncan JR, Schurig GGD, Winter AJ: Bovine venereal
vibriosis: Ultrastructure of endometrial lesions. Lab Invest 33:187-192, 1975.
Corbeil LB, Duncan JR, Winter AJ: Bovine venereal vibriosis: Animal model for
gonorrhea. Bull Comp Pathol 7:2, 1975.
George JW, Corbeil LB, Duncan JR, Fabricant J: A rheumatoid-like arthritis in calves.
Cornell Vet 66:110-117, 1976.
Corbeil LB, George JW, Shively JN, Duncan JR, Schultz R: Canine visceral
leishmaniasis with amyloidosis: An immunopathological case study. Clin Immunol
Immunopathol 6:165-173, 1976.
Corbeil LB, Hall CE, Lein D, Corbeil RR, Duncan JR, Winter AJ: Immunoglobulin
classes in genital secretions of mycoplasma infected and normal heifers. Infect Immunity
12:1595-1600, 1976.
Corbeil LB, Hall CE, Bier P, Duncan JR, Winter AJ: Immune response to genital
mycoplasmosis in bulls. Theriogenol 6:39-44, 1976.
Corbeil LB, Wunderlich AC, Braude AI: Technique for transcervical intrauterine
inoculation of the mouse. Lab Ani Sci 28:314-316, 1978.
Corbeil LB, Wunderlich AC, McCutchan JA, Braude AI: A murine model of gonococcal
bacteremia, in Brooks GF et. al. (eds): Immunobiology of Neisseria gonorrhoeae.
Washington, DC, Amer Soc Microbiol, 1978, p 317-318.
Corbeil LB, Winter AJ: Animal model for the study of genital immune mechanisms:
Venereal vibriosis of cattle, in Brooks GF et al (eds): Immunobiology of Neisseria
gonorrhoeae. Washington, DC, Amer Soc Microbiol, 1978, p 293-299.
Braude AI, Corbeil LB, Levine S, Ito J, McCutchan JA: The possible influence of cyclic
menstrual changes on resistance to the gonococcus, in Brooks GF et al (eds):
Immunobiology of Neisseria gonorrhoeae. Washington, DC, Amer Soc Microbiol, 1978,
p 328-337.
Corbeil LB: The role of complement in immunity and immunopathology. Vet Clin N
Am 8:585-611, 1978.
Corbeil LB, Wunderlich AC, Ito JI, McCutchan JA: Plaque assay for measuring serum
bactericidal activity against gonococci. J Clin Micro 8:618-620, 1978.
Ito JI, Corbeil LB, Wunderlich AC, McCutchan JA, Braude AI: Common heat stable
protective antigen in gonococci. Trans Assoc Am Physicians 92:160-168, 1979.
Corbeil LB, Wunderlich AC, Corbeil RR, McCutchan JA, Ito JI, Braude AI.
Disseminated gonococcal infection in mice. Infect Immun 26:984-990, 1979.
Corbeil LB: Criteria for the development of animal models of human diseases of the
reproductive system. Am J Pathol 101:S241-254, 1980.
Streeter PR, Corbeil LB: Gonococcal infection in endotoxin resistant and endotoxin
susceptible mice. Infect Immunity 32:105-110, 1981.
Corbeil LB, Schurig GG, Duncan JR, Wilkie BN, Winter AJ: Immunity in the female
bovine reproductive tract based on the response to Campylobacter fetus, in JE Butler
(ed): The Ruminant Immune System. Plenum Press. Adv Exp Med Biol 137:729-743,
1981.
Marks MI, Ziegler EJ, Douglas H, Corbeil LB, Braude AI: Induction of immunity
against lethal Haemophilus influenzae Type B infection by Escherichia coli core
lipopolysaccharide. J Clin Invest 69:742-749, 1982.
Harriman GR, Podack ER, Braude AI, Corbeil LB, Esser AF, Curd JG: Activation of
complement by serum-resistant Neisseria gonorrhoeae: Assembly of the membrane
36.
37.
38.
39.
40.
41.
42.
43.
44.
45.
46.
47.
48.
49.
50.
51.
52.
53.
54.
55.
attach complex without subsequent cell death. J Exp Med 156:1235-1249, 1982.
Skaletsky E, Corbeil LB, Wunderlich AC, Sell S, Strayer DS: Proliferative responses of
rabbit lymphocytes to Pasteurella multocida decrease with prolonged immunization.
Infect Immunity 38:383-385, 1982.
Marks MI, Ziegler EJ, Douglas H, Corbeil LB: Lethal Haemophilus influenzae type B
infection in mice. Infection 10:261-266, 1982.
Corbeil LB, Strayer DS, Skaletsky E, Wunderlich AC, Sell S: Immunity to pasteurellosis
in compromised rabbits. Am J Vet Res 44:845-850, 1983.
Strayer DS, Skaletsky E, Cabirac GF, Sharp PA, Corbeil LB, Sell S, Leibowitz JL: A
malignant rabbit fibroma virus causes secondary immunosuppression in rabbits. J
Immunol 130:399-404, 1983.
Biggs WH, Wunderlich AC, Corbeil LB, Davis CE, Curd JG: The effects of penicillinase
on the bactericidal activity and the complement activity in normal human serum.
Antimicrobial Agents Chemotherapy 23:738-741, 1983.
Donnelly SG, Corbeil LB: A note on the shedding of Salmonella tucson by racing
horses. Prevent Vet Med 1:383-388, 1983.
Streeter PR, Wunderlich AC, Corbeil RR, Corbeil LB: Natural immunity to murine
gonococcal bacteremia: Roles of complement, leucocytes and sex. Can J Microbiol
29:331-337, 1983.
Ward ACS, Corbeil LB, Mickelsen WD, Sweet VF: A selective medium for
Gram-negative pathogens from bovine respiratory and reproductive tracts. Amer Assoc
Vet Lab Diagn 26:103-112, 1983.
Bustad LK, Corbeil LB: Human-animal bond and one medicine. In: Ryder OA and
Boyd ML (eds): One Medicine. Springer-Verlag. Berlin, 1984, p 345-352.
Corbeil LB, Watt B, Corbeil RR, Brownson RK, Morrill JL: Immunoglobulin
concentrations in serum and nasal secretions of calves at the onset of pneumonia. Am J
Vet Res 45:773-778, 1984.
Corbeil LB, Wunderlich AC, Lyons JM, Braude AI: Specific cross protective
antigonococcal immunity in the murine genital tract. Can J Microbiol 30:482-487, 1984.
Ward ACS, Corbeil LB, Everman JF: Pasteurellosis ... a fatal link in bovine abortions?
Vet. Med. 80:118-122, 1985.
Corbeil LB, Chatterjee A, Foresman L, Westfall A: Ultrastructure of cyclic changes in
the murine uterus, cervix and vagina. Tissue and Cell 17:53-68, 1985.
Corbeil LB, Woodward WD, Ward ACS, Mickelsen WD, Paisley LG: Bacterial
interactions in bovine respiratory and reproductive infections. J Clin Microbiol
21:803-807, 1985.
Corbeil LB, Blau K, Prieur DJ, Ward ACS: Serum susceptibility of Haemophilus somnus
from clinical cases and carriers. J Clin Microbiol 22:192-198, 1985.
Corbeil LB, Gogolewski RP: Bacterial lung injury. Vet Clin N Am 1:367-376, 1985.
Corbeil LB, Widders PR, Gogolewski R, Arthur J, Inzana TJ, Ward ACS: Haemophilus
somnus: bovine reproductive and respiratory disease. Can Vet J 27:90-93, 1986.
Widders PR, Paisley LG, Gogolewski RP, Evermann JF, Smith JW, Corbeil LB:
Experimental abortion and the systemic immune response in cattle to Haemophilus
somnus. Infect Immun 54:555-560, 1986.
Sell S, Strayer D, Skaletsky E, Corbeil L, Cabirac G, Leibowitz J: Leporine acquired
immune deficiency disease. Symp Fund. Cancer Res. 38:97-111, 1986.
Morris DD, Cullor JS, Whitlock RH, Wickstrom M, Corbeil LB: Endotoxemia in
neonatal calves given antiserum to a mutant of Escherichia coli (J5). Am J Vet Res
56.
57.
58.
59.
60.
61.
62.
63.
64.
65.
66.
67.
68.
69.
70.
71.
72.
73.
74.
47:2554-2564, 1986.
Wickstrom ML, Gay CC, Hodgson JL, Widders PR, Schaeffer D, Lee R, and Corbeil LB:
Cross reactive antibody in immunity to colisepticemia in calves. Vet Microbiol
13:259-271, 1987.
Inzana TJ, Corbeil LB: Further characterization of the growth requirements of
Haemophilus somnus: Development of a defined medium. Am J Vet Res 48:366-369,
1987.
Gogolewski RP, Leathers CW, Liggitt HD, Corbeil LB: Experimental Haemophilus
somnus pneumonia in calves and immunoperoxidase localization of bacteria. Vet Pathol
24:250-256, 1987
Blau KA, Ward ACS, Prieur DJ, Corbeil LB: Serum susceptibility of bovine
pasteurellas. Can J Vet Res 51:157-161, 1987.
Woodward WD, Besser TE, Ward ACS, Corbeil LB: In vitro inhibition of mastitis
pathogens by bovine teat skin normal flora. Can J Vet Res 51:27-31, 1987.
Gogolewski RP, Kania SA, Inzana TJ, Widders PR, Liggitt HD, Corbeil LB: Protective
ability and specificity of convalescent serum from calves with Haemophilus somnus
pneumonia. Infect Immun 55:1403-1411, 1987.
Corbeil LB, Arthur JE, Widders PR, Smith JW, Barbet AF: Antigenic specificity of
convalescent serum from cattle with Haemophilus somnus experimental abortion. Infect
Immun 55:1381-1386, 1987.
Fox LK, McDonald JS, Corbeil LB: Milk neutrophil phagocytosis of Staphylococcus
aureus as determined by fluorescent microscopy. J Vet Med 34:549-551, 1987.
Corbeil LB: Strategies to overcome bacterial virulence mechanisms: an overview. In:
J.A. Roth (ed). Virulence Mechanisms of Bacterial Pathogens. pp 301-310 American
Society for Microbiology Publications, 1988.
Al-Lebban ZS, Corbeil LB, Coles EH: Rabbit pasteurellosis: Induced disease and
vaccination. Am J Vet Res 49:312-316, 1988.
Fox AK, McDonald JS, Hillers JK and Corbeil LB: Function of phagocytes obtained
from lacteal secretions of lactating and nonlactating cows. Am J Vet Res 49:678-681,
1988.
Gogolewski RP, Kania SA, Liggitt HD, Corbeil LB: Protective ability of monospecific
sera against 78-kDa and 40-kDa outer membrane antigens of "Haemophilus somnus".
Infect Immun 56:2307-2316, 1988.
Yarnall M, Widders PR, Corbeil LB: Isolation and characterization of Fc receptors from
Haemophilus somnus. Scand J Immunol 28:129-137, 1988.
Woodward WD, Fox LK, Ward ACS, Corbeil LB: Teat skin normal flora and
colonization with mastitis pathogen inhibitors. Vet Microbiol 17:357-365, 1988.
Widders PR, Smith JW, Yarnall M, McGuire TC, Corbeil LB: Non-immune
immunoglobulin binding of Haemophilus somnus. J Med Micro 26:307-311, 1988.
Corbeil LB, Chikami G, Yarnall M, Smith J., Guiney DG: Cloning and expression of
genes encoding for Haemophilus somnus antigens. Infect Immun 56:2736-2742, 1988.
Yarnall M, Gogolewski RP, Corbeil LB: Characterization of two Haemophilus somnus
Fc receptors. J Gen Microbiol 134:1993-1999, 1988.
Corbeil LB, Blau K, Inzana TJ, Nielsen KH, Jacobson RH, Corbeil RR, Winter AJ:
Killing of Brucella abortus by bovine serum. Infect Immun 56:3251-3261, 1988.
Inzana TJ, Iritani B, Gogolewski RP, Kania SA, Corbeil LB: Purification and
characterization of lipooligosaccharides from four strains of "Haemophilus somnus".
Infect Immun 56:2830-2837, 1988.
75.
76.
77.
78.
79.
80.
81.
82.
83.
84.
85.
86.
87.
88.
89.
90.
91.
92.
93.
94.
Yarnall M, Corbeil LB.: Antibody response to Haemophilus somnus Fc receptor. J Clin
Microbiol 27:111-117, 1989.
Widders PR, Dorrance L, Yarnall M, Corbeil LB: Immunoglobulin binding activity
among pathogenic and carrier isolates of Haemophilus somnus. Infect Immun
57:639-642, 1989.
Corbeil LB, Hodgson JL, Jones DW, Corbeil RR, Widders PR, Stephens LR: Adherence
of Tritrichomonas foetus to bovine vaginal epithelial cells. Infect Immun 57:2158-2165,
1989.
Gogolewski RP, Schaefer DC, Wasson SK, Corbeil RR, Corbeil LB: Pulmonary
persistence of Haemophilus somnus in presence of specific antibody. J Clin Microbiol
27:1767-1774, 1989.
Widders PR, Dowling SC, Gogolewski RP, Smith JW, Corbeil LB: Isotypic antibody
responses in cattle infected with Haemophilus somnus. Res Vet Sci 46:212-217, 1989.
Aydintug MK, Inzana TJ, Letonja T, Davis WC, Corbeil LB. Cross-reactivity of
monoclonal antibodies to Escherichia coli J5 with heterologous Gram- negative bacteria
and extracted lipopolysaccharides. J Infect Dis 160:846-857, 1989.
Kania SA, Gogolewski RP, Corbeil LB: Characterization of a 78 kDa outer membrane
protein of Haemophilus somnus. Infect Immun 58:237-244, 1990.
Corbeil LB: Molecular aspects of some virulence factors of Haemophilus somnus. Can J
Vet Res 54:S57-S62, 1990.
Fox LK, Liggitt HD, Yilma T, Corbeil LB: The effect of interferon gamma
intramammary administration on mammary phagocyte function. J Vet Med B 37:28-30,
1990.
Hodgson JL, Jones DW, Widders PR, Corbeil LB: Specificity and function of
monoclonal antibodies to Tritrichomonas foetus. Infect Immun 58:3078-3083, 1990.
Corbeil LB: Immunity to Infectious Diseases. Chapter 7. In: Woolcock JB (ed): World
Animal Science. Vol. 6: Microbiology of Animals and Animal Products. p 115-139
Elsevier, New York 1991.
Talbot JA, Nielsen K, Corbeil LB. Cleavage of proteins of reproductive secretions by
extracellular proteinases of Tritrichomonas foetus. Can J Microbiol 37:384-390, 1991.
Corbeil LB, Kania SA, Gogolewski RP. Characterization of immunodominant surface
antigens of Haemophilus somnus. Infect Immun 59:4295-4301, 1991.
Corbeil LB, Hodgson JL, Widders PR. Immunoglobulin binding by Tritrichomonas
foetus. J Clin Microbiol 29:2710-2714, 1991.
Cole SP, Guiney DG, Corbeil LB. Two linked genes for outer membrane proteins are
absent in four non-disease strains of Haemophilus somnus. Molec Microbiol 6:18951902, 1992.
Inzana TJ, Gogolewski RP, Corbeil LB. Phenotypic phase variation in Haemophilus
somnus lipooligosaccharide during bovine pneumonia and after in vitro passage. Infect
Immun 60:2943-2951, 1992.
BonDurant RH, Corbeil RR, Corbeil LB. Immunization of virgin cows with a surface
antigen of Tritrichomonas foetus. Infect Immun 61:1385-1394, 1993.
Cole SP, Guiney DG, Corbeil LB. Molecular analysis of a gene encoding a serum
resistance associated 76 kDa surface antigen of Haemophilus somnus. J Gen Microbiol
139:2135-2143, 1993.
Ikeda, JS, BonDurant RH, Campero CM, Corbeil LB. Conservation of a protective
surface antigen of Tritrichomonas foetus. J Clin Microbiol 31:3289-3295, 1993.
Corbeil LB. Vaccination strategies against Tritrichomonas foetus. Parasitology Today
95.
96.
97.
98.
99.
100.
101.
102.
103.
104.
105.
106.
107.
108.
109.
110.
111.
112.
10:103-106, 1994.
Lees VW, Yates WDG, Corbeil LB. Ovine Haemophilus somnus: Experimental
infection and antigenic comparison with bovine Haemophilus somnus. Can J Vet Res
58:202-210, 1994.
Ikeda JS, BonDurant RH, Corbeil LB. Bovine vaginal antibody responses to an
immunoaffinity-purified surface antigen of Tritrichomonas foetus. J Clin Microbiol
33:1158-1163, 1995.
Ward ACS, Jaworski MD, Eddow JM, Corbeil LB. A comparative study of bovine and
ovine Haemophilus somnus isolates. Can J Vet Res 59:173-178, 1995.
Corbeil LB. Use of an animal model of trichomoniasis as a basis for understanding this
disease in women. Clin Infect Dis 21 (Suppl 2):S158-161, 1995.
Corbeil LB, Gogolewski RP, Stephens LR, Inzana TJ. Haemophilus somnus: Antigen
analysis and immune responses. In: Haemophilus, Actinobacillus and Pasteurella.
Donachie W (Ed). Plenum Press, New York p 63-73, 1995.
Thomford JW, Talbot JA, Ikeda JS, Corbeil LB. Characterization of extracellular
proteinase of Tritrichomonas foetus. J Parasitol 82:112-117, 1996.
Anderson ML, BonDurant RH, Corbeil RR, Corbeil LB. Immune and inflammatory
responses to reproductive tract infection with Tritrichomonas foetus in immunized and
control heifers. J Parasitol 82:594-600, 1996.
BonDurant RH, Van Hoosear KA, Corbeil LB, Bernoco D. Serological response to in
vitro-shed antigen(s) of Tritrichomonad foetus in cattle. Clin Diagn Lab Immunol 3:432437, 1996.
Corbeil LB, Bastida-Corcuera FD, Beveridge TJ. Haemophilus somnus immunoglobulin
binding proteins and surface fibrils. Infect Immun 65:4250-4257, 1997.
Corbeil LB, Gogolewski RP, Kacskovics I, Nielsen KH, Corbeil RR, Morrill JL,
Greenwood R and Butler JE. Bovine IgG2a antibodies to Haemophilus somnus and
allotype expression. Can J Vet Res 61:207-213, 1997.
Sanders JD, Tagawa Y, Briggs RE, Corbeil LB. Transformation of a virulence associated
gene of Haemophilus somnus into a strain lacking the gene. FEMS Microbiol Letters
154:251-258, 1997.
Mutwiri GK, Corbeil LB. Genital and systemic immune responses in a murine model of
Tritrichomonas foetus infection. J Parasitol 84:321-327, 1998 .
Corbeil LB, Anderson ML, Corbeil RR, Eddow JM, BonDurant RH. Female
reproductive tract immunity in bovine trichomoniasis. Am J Reprod Immunol 39:189198, 1998.
Corbeil LB, BonDurant RH. Bovine trichomoniasis. In: Sexually Transmitted Diseases
and Adverse Outcomes of Pregnancy. (Eds) Hitchock PJ, MacKay T, Wasserheit JN.
ASM Press. Washington DC. p 311-327, 1999.
Bastida-Corcuera F, Butler JE, Yahiro S, Corbeil LB. Differential complement activation
by bovine IgG2 allotypes. Vet Immunol Immunopathol 71:115-123, 1999.
Rhyan JC, Wilson KL, Wagner B, Anderson ML, BonDurant RH, Burgess DE, Mutwiri
GK, Corbeil LB. Demonstration of Tritrichomonas foetus in the external genitalia and of
specific antibodies in smegma of naturally-infected bulls. Vet Pathol 36:406-411, 1999.
Ward ACS, Dyer NW, Corbeil LB. Characterization of putative Haemophilus somnus
isolates from tonsils of American Bison (Bison bison). Can J Vet Res 63:166-169, 1999.
Bastida-Corcuera F, Nielsen KH, Corbeil LB. Binding of bovine IgG2a and IgG2b
allotypes to protein A, protein G, and Haemophilus somnus IgBPs. Vet Immunol
Immunophathol 71:143-149, 1999.
113.
114.
115.
116.
117.
118.
119.
120.
121.
122.
123.
124.
125.
127.
127.
128.
129.
130.
131.
Corbeil LB. Immunization and diagnosis in bovine reproduction tract infections. In:
Schultz RD (ed). Veterinary Vaccines and Diagnostics. Adv Vet Med 41:217-239, 1999.
Tagawa Y, Bastida-Corcuera F, Corbeil LB. Immunological characterization of the
major outer membrane protein of Haemophilus somnus. Veterinary Microbiol 71:245254, 2000.
Bastida-Corcuera F, Butler JE, Heyermann H., Thomford JW, Corbeil LB.
Tritrichomonas foetus extracellular cysteine proteinase cleavage of bovine IgG2
allotypes. J. Parasitol. 86:328-332, 2000.
Corbeil, LB. Bacterial resistance to antibody-dependent defenses. In: Virulence
Mechanisms of Bacterial Pathogens, 3rd Edition, Eds K.A. Brogden et. al., ASM Press,
Washington, D.C., pp119-130, 2000.
Mutwiri GK, Henk WG, Enright FM, Corbeil, LB. Effect of the antimicrobial peptide,
D-hecate, on trichomonads. J. Parasitol. 86:1355-1359, 2000.
Singh BN, BonDurant RH, Campero CM, Corbeil LB. Immunological and biochemical
analysis of glycosylated surface antigens and lipophosphoglycan of Tritrichomonas
foetus. J. Parasitol. 87:770-777, 2001.
Kania SA, Reed SL, Thomford JW, BonDurant RH, Hirata K, Corbeil RR, North MJ,
Corbeil LB. Degradation of bovine complement C3 by trichomonad extracellular
proteinase. Vet. Immunol. Immunopathol. 78:83-96, 2001.
Corbeil LB, Munson L, Campero C, BonDurant RH. Bovine trichomoniasis as a model
for development of vaccines against sexually-transmitted disease. Am. J. Reprod.
Immunol. 45:310-319, 2001.
Sylte MJ, Corbeil LB, Inzana TJ, Czuprynski CJ. Haemophilus somnus induces
apoptosis in bovine endothelial cells in vitro. Infect. Immun. 69:1650-1660, 2001.
Corbeil LB, BonDurant RH. Immunity to Bovine Reproductive Infections. In: J. Roth
(ed). Veterinary Clinics of North America: Food Animal Practice. 17:567-583, 2001.
Grab DJ, Lonsdale-Eccles JD, Oli MW, Corbeil, LB. Lactoferrin-binding proteins of
Tritrichomonas foetus. J. Parasitol. 1064-1070, 2001
Corbeil, LB. Antibodies as effectors. Vet Immunol Immunopathol 87:169-175, 2002.
Sanders JD, Bastida-Corcuera FD, Arnold KF, Wunderlich AC, Corbeil LB. Genetic
manipulation of immunoglobulin binding proteins of Haemophilus somnus. Microbial
Pathog. 34:131-139, 2003.
Corbeil LB, Campero CM, Rhyan JC, BonDurant RH. Vaccines against sexually
transmitted diseases. Reprod Biol Endrocrinol. 1:118-123, 2003.
Hodkins DC, Yuan L, Parreno V, Corbeil LB, Saif LJ. Mucosal Veterinary Vaccines. In
Mucosal Immunology (Eds) Mestecky J, Bienenstock J, Lamm ME, Mayer L, McGee JR,
Strober W. Elsevier Inc. 2004.
Inzana T, Corbeil LB. Haemophilus. In Pathogenesis of Bacterial Pathogens of Animals.
3rd Edition. (Eds) Gyles CL, Thoen CO, Prescott JF. Pg 243 – 257. Blackwell
Publishing Professional, Ames IA. 2004.
Kuckleburg C, Sylte MJ, Inzana TJ, Corbeil LB, Daries B, Czuprynski CJ. Bovine
platelets activated by Haemophilus somnus or its lipooligosaccharide cause endothelial
cell damage in vitro. Microb Pathog. 38:23-32, 2005.
Czuprynski CJ, Leite F, Sylte M, Kunckleburg C, Schultz R, Inzana T, Corbeil L.
Complexities of the Pathogenesis of Mannheimia haemolytica and Haemophilus somnus
infections: challenges and potential opportunities for prevention? Animal Health Res Rev
5:277-282, 2005.
Corbeil LB, Campero CM, Rhyan JC, Anderson ML, Gershwin LJ, Agnew DW, Munson
L, Bondurant RH. Uterine mast cells and IgE antibody responses during clearance of
132.
133.
134.
135.
136.
137.
138.
139.
140.
141.
142.
143.
144.
145.
146.
147.
148.
Tritrichomonas foetus. Vet Pathol. 42:283-290, 2005.
Gershwin LJ, Berghaus LJ, Arnold K, Anderson ML, Corbeil LB. Immune mechanisms
of pathogenetic synergy in concurrent bovine pulmonary infection with Haemophilus
somnus and bovine respiratory syncytial virus. Vet Immunol Immunopathol 107:119130, 2005.
Tagawa Y, Sanders JD, Uchida I, Bastida-Corcuera FD, Kawashima K, Corbeil LB.
Genetic and functional analysis of Haemophilus somnus high molecular weightimmunoglobulin binding proteins. Microbial Pathog. 39:159-170, 2005.
Ward ACS, Weiser GC,. Anderson BC, Cummings PJ, Arnold KF, Corbeil LB.
Haemophilus somnus(Histophilus somni) in Bighorn Sheep. Can J Vet Res. 70:34-42,
2006.
Berghaus LJ, Corbeil LB, Berghaus RJ, Kalina WB, Gershwin LJ. Simultaneous
vaccination for bovine respiratory synctial virus and Haemophilus somnus protects
against H. somnus but not BRSV. Vaccine 24:6018-6027, 2006.
Corbeil LB, Arnold KF, Kimball R, Berghaus L, Gershwin LJ. Specificity of IgG and
IgE antibody responses to Haemophilus somnus infection of calves. Vet Immunol
Immunopathol 113:191-199, 2006.
Behling-Kelly E, Vonderheid H, Kim Kwang Sik, Corbeil LB, Czuprynski CJ. Roles of
cellular activation and sulfated glycans in Haemophilus somnus adherence to bovine
brain microvascular endothelial cells. Infect Immun 74:5311-5218, 2006.
Geertsema RS, Kimball RA, Corbeil LB. Bovine plasma proteins increase virulence of
Haemophilus somnus in mice. Microb Pathog 42:22-28, 2007.
Cobo ER, Corbeil LB, Agnew DW, VanHoosear K, Friend A, Olesen DR, BonDurant
RH. Tetratrichomonas spp. and Pentatrichomonas hominis are not persistently
detectable after intravaginal inoculation of estrous heifers. Vet Parasitol 150:18-26,
2007.
Agnew DW, Munson L, Cobo ER, Olesen D, Corbeil LB, BonDurant RH. Comparative
histopathology and antibody responses of non-Tritrichomonas foetus trichomonad and
Tritrichomonas foetus genital infections in virgin heifers. Vet Parasitol 151:170-180,
2008
Corbeil LB. Histophilus somni host-parasite relationships. An Hlth Res Rev 8:151-160,
2008.
Corbeil LB, Campero CM, Van Hoosear K, BonDurant RH. Detection of trichomonad
species in the reproductive tracts of breeding and virgin bulls. Vet Parasitol 154: 226232, 2008.
Geertsema RS, Herdman DS, Lo K, Kimball RA, Russo R, Dixon J, Worby C, Tagawa
Y, Corbeil LB. Protection of mice against H. somnus septicemia by vaccination with
recombinant immunoglobulin binding protein subunits. Vaccine 26:4506-4512, 2008
Agnew DW, Corbeil LB, Munson L, Byrne BA, R.H. BonDurant RH. A pregnant mouse
model for bovine Tritrichomonas foetus infection. Vet Pathol 45:849-864, 2008
Hoshinoo K, Sasaki K, Tanaka A, Corbeil LB, Tagawa Y. Virulence attributes of
Histophilus somni with a deletion mutation in the ibpA gene. Microb Pathog. 46: 273282, 2009
Worby CA, Mattoo S, Kruger RP, Corbeil LB, Koller A, Mendez JC, Zekarias B, Lazer
C, Dixon JE. The Fic domain: regulation of cell signaling by adenylylation. Molec Cell
34:93-103, 2009
O’Toole D, Allen T, Hunter R, Corbeil LB, Diagnostic Exercise: Acute myocarditis and
histophilosis in feedlot cattle. Vet Pathol 46:1015–1017, 2009
Cobo ER, Corbeil LB, Gershwin LJ, BonDurant RH. Preputial cellular and antibody
149.
150.
151.
152.
153.
154.
responses of bulls vaccinated and/or challenged with Tritrichomonas foetus. Vaccine 28:
361-370, 2010
Zekarias B, Mattoo S, Worby C, Lehmann J, Rosenbusch RF, Corbeil LB. Histophilus
somni IbpA DR2/Fic in virulence and immunoprotection at the natural host alveolar
epithelial barrier. Infect Immun 78:1850-1858, 2010
Zekarias B, O’Toole, Lehmann J, Corbeil LB. Histophilus somni IbpA cytotoxin is
conserved in disease strains and most carrier strains from cattle, sheep and bison. Vet
Microbiol 149: 177-185, 2011.
Geertsema RS, Zekarias B, Scheuch LL, Worby C, Russo R, Gershwin LJ, Herdman DS,
Lo K, Corbeil LB. IbpA DR2 subunit immunization protects calves against Histophilus
somni pneumonia. Vaccine 29: 4805-4812, 2011.
Cobo ER, Eckmann L, Corbeil LB. Murine models of vaginal trichomonad infections.
Am J Trop Med Hyg 85:667–73, 2011.
Cobo ER, Reed SL, Corbeil LB. Effect of vinyl sulfone inhibitors of cysteine proteinases
on Tritirichomonas foetus infection. Internat J Antimicrobial agents 39: 259-262, 2012.
Lo KL, Kimball RA, Lehmann J, Gershwin LJ, Worby C, Corbeil LB. Antibody
responses of calves to Histophilus somni recombinant IbpA subunits. Comp Immunol
Microbiol Infectious Dis. 35:453-459, 2012.