Supplemental Digital Content 1 R2 of regression analyses using PBF estimated from published equations using anthropometric measures to predict PBF from DXA in NHANES 1999-2004 in adult males and females matched by age range and race/ethnicity with the sample in which the equations were developed 1-3 Males Anthropometric(s) used in equation/ Equation evaluated Triceps and subscapular skinfold thickness Durnin and Womersley age-specific (5) 20 - 39 years 40 - 59 years 60 - 68 years 20 - 68 years Durnin and Womersley all-age (5) 20 - 39 years 40 - 59 years 60 - 68 years 20 - 68 years Hassager et al. (11) 20 - 39 years 40 - 59 years 60 - 72 years 20 - 72 years Slaughter et al. (30) 20 - 29 years Height, weight and/or body mass index (BMI) Deurenberg et al., 1989 (2) 60 - 83 years Deurenberg et al.1991 (3) 20 - 39 years 40 - 59 years 60 - 83 years 20 - 83 years Deurenberg et al.1998 4 Gallagher et al. 1996 (9) 5 20 - 39 years 40 - 59 years 60 - 84 years 20 - 84 years Gallagher et al. 2000 full (8) 5 20 - 39 years 40 - 59 years 60 - 84 years 20 - 84 years Gallagher et al. 2000 simplified (8) 5 20 - 39 years 40 - 59 years 60 - 84 years 20 - 84 years 1 2 Females n R2 n R 799 808 338 1,945 0.689 0.587 0.433 0.617 724 693 338 1,755 0.671 0.630 0.460 0.640 799 808 338 1,945 0.750 0.580 0.433 0.661 724 693 338 1,755 0.678 0.634 0.460 0.634 799 808 505 2112 0.745 0.585 0.432 0.684 724 693 481 1898 0.677 0.646 0.429 0.658 591 0.770 493 0.665 945 0.474 878 0.558 799 808 945 2,552 0.567 0.498 0.497 0.568 724 693 878 2,295 0.644 0.629 0.521 0.628 1,152 1,129 1,225 3,506 0.570 0.509 0.504 0.592 1,014 918 1,158 3,090 0.649 0.618 0.538 0.654 1152 1129 1225 3506 0.575 0.511 0.510 0.601 1014 918 1158 3090 0.676 0.672 0.613 0.701 1152 1129 1225 3506 0.563 0.498 0.502 0.591 1014 918 1158 3090 0.671 0.665 0.606 0.694 Gomez-Ambrosi et al. (10) 20 - 39 years 40 - 59 years 60 - 80 years 20 - 80 years Heitmann (12) 35 - 65 years Jackson et al. (16) 20 - 39 years 40 - 65 years 20 - 65 years Noppa et al. (23) 44 - 66 years Pasco et al. (25) 5 20 - 39 years 40 - 59 years 60 - 84 years 20 - 84 years Rush et al. (28) 20 - 27 years Smith et al.(31) 25 - 37 years Visser et al. (33) 5 60 - 84 years Waist circumference (WC) or waist-to-height ratio (WHtR) Kagawa et al. (17) 20 - 39 years 40 - 64 years 20 - 64 years Lean et al. WC (19) 20 - 39 years 40 - 64 years 20 - 64 years Multiple anthropometric variables 6 Chapman et al. (1) 5 76 - 84 years Lean et al. WC-tricpes (19) 20 - 39 years 40 - 64 years 20 - 64 years Ramirez-Zea et al. (27) 20 - 39 years 40 - 56 years 20 - 56 years Svendsen et al. (32) 75 years Wilmore and Behnke body density (35) 20 - 36 years 2 799 808 816 2,423 0.597 0.502 0.494 0.603 724 693 745 2,162 0.685 0.668 0.578 0.698 1,218 0.514 1,114 0.683 1,152 1,440 2,592 0.577 0.519 0.585 1,014 1,237 2,251 0.652 0.648 0.671 - - 811 0.541 799 808 982 2,589 0.600 0.494 0.485 0.582 724 693 930 2,347 0.688 0.648 0.616 0.672 - - 284 0.721 - - 463 0.643 982 0.470 930 0.549 799 996 1795 0.738 0.606 0.682 724 885 1609 0.591 0.543 0.563 799 996 1,795 0.707 0.616 0.680 724 885 1,609 0.554 0.559 0.553 348 0.463 343 0.459 799 996 1,795 0.795 0.666 0.738 724 885 1,609 0.686 0.697 0.692 475 318 793 0.713 0.629 0.692 350 269 619 0.538 0.508 0.551 45 0.567 37 0.570 684 0.757 - - Wilmore and Behnke lean body weight (35) 20 - 36 years 684 1 0.749 - - Values were adjusted for complex sample design and expressed as the average of the five R2s respectively from the five imputation DXA datasets. 2 Only non-Hispanic Whites were included in the analyses. The exceptions were equations of Slaughter et al., Jackson et al., Gallagher et al. 1996, Gallagher et al. 2000 full and simplified for which both non-Hispanic Whites and Blacks were included, and the Ramirez-Zea et al. equation for which only Mexican Americans were included. 3 Abbreviations: DXA for dual-energy X-ray absorptiometry, NHANES for National Health and Nutrition Examination Survey, and PBF for percentage body fat. 4 The Deurenberg 1998 equation was excluded for analysis because age ranges were unknown. 5 Analyses were limited to sample aged ≤ 84 years since NHANES coded the age of those aged ≥ 85 as 85 years. 6 Anthropometrics used in equations were waist circumference and triceps skinfold for Lean et al. WC-triceps equation, BMI and triceps skinfold for Svendsen et al. equation, weight, height and triceps skinfold for Chapman et al. equation, weight and waist circumference for Wilmore and Behnke body density and lean body weight equations, and weight, height and waist circumference for Ramirez-Zea et al. equation. 3