Table S8: Significantly over or underrepresented subsystems (level III) in Troll metagenomes compared to both metagenomes from the
Oslofjord
Level III subsystems differing significantly in one or more Troll metagenomes compared to both Oslofjord metagenomes after statistical analysis
in STAMP.
Underrepresented compared to the Oslofjord
Overrepresented compared to the Oslofjord
level I
level III
Troll samples
level I
level III
Amino Acids and
Ketoisovalerate
Tplain and
Amino Acids and
Cyanophycin metabolism Tplain and
Derivatives
oxidoreductase
Tpm1-2
Derivatives
Carbohydrates
N-Acetyl-Galactosamine and
Tplain and
Amino Acids and
Galactosamine Utilization
Tpm1-2
Derivatives
L-ascorbate utilization (and
Tplain and
Amino Acids and
related gene clusters)
Tpm1-2
Derivatives
L-fucose utilization
Tpm1-2
Amino Acids and
Choline and Betaine
Tplain and
Derivatives
Uptake and Betaine
Tpm1-2
Carbohydrates
Carbohydrates
Troll samples
Tpm1-2
Glutamine synthetases
Tplain and
Tpm1-2
Methionine Degradation
Tplain and
Tpm1-2
Biosynthesis
Underrepresented compared to the Oslofjord
Overrepresented compared to the Oslofjord
level I
level III
Troll samples
level I
level III
Troll samples
Clustering-based
1
Tpm1-2
Carbohydrates
Methylcitrate cycle
Tplain
subsystems
1T762T79.3.peg.1T2T62T
Clustering-based
1
Tplain
Cell Wall and Capsule
Lipid A modifications
Tplain and
subsystems
1T762T80.1T.peg.1T561T
Clustering-based
1
subsystems
Cofactors, Vitamins,
CBSS-
CBSS-
Tpm1-2
Tplain and
Clustering-based
1
2T5731T4.1T.peg.752T
Tpm1-2
subsystems
32T0388.3.peg.3759
Flavodoxin
Tplain and
Clustering-based
1
Tpm1-2
subsystems
2T2T1T988.1T.peg.771T Tpm1-2
Tpm3
Clustering-based
GABA and putrescine
Tplain and
subsystems
metabolism from cluters
Tpm1-2
Clustering-based
1
Tpm1-2
subsystems
354.1T.peg.2T91T7
CBSS-
Prosthetic Groups,
CBSS-
CBSS-
Tplain
Tplain and
Pigments
DNA Metabolism
DNA Metabolism
ATP-dependent Nuclease
DNA repair and
recombination eukaryotic
Tplain
CBSS-
Underrepresented compared to the Oslofjord
Overrepresented compared to the Oslofjord
level I
level III
Troll samples
level I
level III
Troll samples
DNA Metabolism
DNA replication, archaeal
Tplain
Clustering-based
1
Tplain and
subsystems
891T87.3.peg.2T957
Tpm1-2
Clustering-based
1
Tplain and
subsystems
31T42T69.3.peg.1T840
Tpm1-2
Clustering-based
1
Tplain
subsystems
3931T2T4.3.peg.2T657
Tplain and
Clustering-based
1
Tpm1-2
subsystems
2T0592T2T.3.peg.1T809
1 and Tpm1-2
Tplain, Tpm1-
Cofactors, Vitamins,
Chlorophyll Degradation
Tpm1-2
1, Tpm1-2,
Prosthetic Groups,
Tpm2 and
Pigments
carnitine metabolism
Tpm1-2
DNA Metabolism
DNA topoisomerases, Type I,
Tplain
ATP-independent
DNA Metabolism
Membrane Transport
Motility and
Late competence
ECF class transporters
Archaeal Flagellum
Chemotaxis
Tplain
CBSS-
CBSS-
CBSS-
CBSS-
Tplain, Tpm1-
Tpm3
Nitrogen Metabolism
Nitrogen fixation
Tpm2
Fatty Acids and
Lipids
Underrepresented compared to the Oslofjord
Overrepresented compared to the Oslofjord
level I
level III
Troll samples
level I
level III
Troll samples
Nitrogen Metabolism
Nitrosative stress
Tpm1-2
Macromolecular
1
Tplain
Synthesis
2T432T77.1T.peg.51T1T
Metabolism of
Biphenyl Degradation
Protein Metabolism
Glu-tRNA(Gln)
Tplain
transamidation
Protein Metabolism
Ribosome LSU eukaryotic
Tplain
Ribosome SSU eukaryotic
Tplain
and archaeal
Protein Metabolism
Translation elongation factors
Tplain
eukaryotic and archaeal
Regulation and Cell
signaling
Coagulation cascade
Metabolism of
Phenylpropanoid
Tplain and
Aromatic Compounds
compound degradation
Tpm1-2
Metabolism of
Benzoate transport and
Tplain
Aromatic Compounds
degradation cluster
Metabolism of
Gentisare degradation
Tplain and
Tpm1-2
Aromatic Compounds
Tpm1-2
Tplain and
Tpm1-2
Aromatic Compounds
and archaeal
Protein Metabolism
CBSS-
Metabolism of
Salicylate and gentisate
Tplain and
Aromatic Compounds
catabolism
Tpm1-2
Underrepresented compared to the Oslofjord
Overrepresented compared to the Oslofjord
level I
level III
Troll samples
level I
level III
Troll samples
Respiration
V-Type ATP synthase
Tplain
Miscellaneous
Biolumenescence
Tplain and
Tpm1-2
Respiration
Respiration
Carbon monoxide induced
Tplain and
hydrogenase
Tpm1-2
Reductive Dechlorination
Tpm1-2
Nitrogen Metabolism
Ammonia assimilation
Tplain
Nitrogen Metabolism
Dissimilatory nitrite
Tpm1-2
reductase
RNA Metabolism
Wyeosine-MimG
Tpm1-2
Nitrogen Metabolism
Nitric oxide synthase
Biosynthesis
RNA Metabolism
RNA Metabolism
RNA polymerase archaeal
RNA polymerase archaeal
initiation factors
Tplain, Tpm12 and Tpm2
Tplain
Tplain
Regulation and Cell
DNA-binding regulatory
Tplain and
signaling
proteins, strays
Tpm1-1
Regulation and Cell
Orphan regulatory
Tplain and
signaling
proteins
Tpm1-2
Underrepresented compared to the Oslofjord
Overrepresented compared to the Oslofjord
level I
level III
Troll samples
level I
level III
Troll samples
RNA Metabolism
RNA polymerase II
Tplain, Tpm1-
Respiration
Terminal cytochrome C
Tplain and
oxidases
Tpm1-2
Biogenesis of
Tplain and
cytochrome c oxidases
Tpm1-2
Protection from Reactive
Tplain and
Oxygen Species
Tpm1-2
Stress Response
Flavohaemoglobin
Tplain
Sulfur Metabolism
Inorganic Sulfur
Tplain
2 and Tpm3
Stress Response
Unclassified
Unclassified
Rubrerythrin
Tpm1-2
Acetone Butanol Ethanol
Tplain and
Synthesis
Tpm1-2
CR clusters Euk 1T
Tplain and
Respiration
Stress Response
Tpm1-2
Virulence
Adhesion of Campylobacter
Tpm1-2
Assimilation
Virulence
Heme, hemin uptake and
utilization systems in
GramPositives
Tpm2
Unclassified
Acyclic terpenes
Tplain and
utilization
Tpm1-2
Underrepresented compared to the Oslofjord
Overrepresented compared to the Oslofjord
level I
level III
Troll samples
level I
level III
Troll samples
Virulence
Pseudaminic Acid
Tplain, Tpm1-
Unclassified
PQQ-dependent
Tplain and
Biosynthesis
2 and Tpm3
quinoprotein
Tpm1-2
dehydrogenases
Virulence
Bacterial Endolysins:
Tpm1-2
Unclassified
YgfZ-Fe-S clustering
autolysins, phage, and phage-
Tplain and
Tpm1-2
like lysins
Virulence
Listeria phi-A1T1T8-like
Tpm1-2
prophages
Virulence
Staphylococcal phi-Mu50B-
Hemin transport system
Tplain and
Tpm1-2
Tpm1-2
like prophages
Virulence
Arsenic resistance
Tplain
Virulence
Streptolysin S Biosynthesis
Tpm1-2
and Transport
Virulence
1
Clustering-based subsystems: subsystems in which there is functional coupling evidence that genes belong together, but their exact function is
not known.